AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061618


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061618.t1 MTR_1g044580 98.667 150 2 0 3 152 141 290 2.26e-102 308
MS.gene061618.t1 MTR_1g073320 70.000 160 38 1 3 152 139 298 4.63e-71 217
MS.gene061618.t1 MTR_7g098410 47.651 149 76 2 5 152 135 282 1.39e-41 140
MS.gene061618.t1 MTR_1g080350 37.333 150 92 2 6 154 147 295 1.39e-27 104
MS.gene061618.t1 MTR_1g044560 43.689 103 58 0 4 106 24 126 2.62e-22 86.3
MS.gene061618.t1 MTR_8g104990 37.241 145 87 3 9 151 138 280 1.28e-19 83.2
MS.gene061618.t1 MTR_3g065370 38.384 99 56 2 53 149 177 272 5.27e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061618.t1 AT1G04520 64.072 167 45 3 3 154 141 307 3.45e-66 204
MS.gene061618.t1 AT2G33330 62.346 162 51 2 3 154 143 304 1.29e-63 197
MS.gene061618.t1 AT3G04370 41.611 149 84 2 6 153 172 318 1.56e-34 123
MS.gene061618.t1 AT3G04370 41.611 149 84 2 6 153 171 317 1.89e-34 122
MS.gene061618.t1 AT5G43980 39.752 161 87 3 3 154 141 300 2.84e-33 119
MS.gene061618.t1 AT3G60720 33.557 149 84 5 6 150 143 280 3.73e-16 73.9
MS.gene061618.t1 AT3G60720 33.793 145 81 5 6 146 143 276 1.51e-15 72.0
MS.gene061618.t1 AT2G01660 38.298 94 53 2 53 143 185 276 5.01e-12 62.4
MS.gene061618.t1 AT2G01660 38.298 94 53 2 53 143 185 276 5.29e-12 62.4

Find 28 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTATCAGCGATGGAAAA+TGG 0.195405 1.2:-27582038 MS.gene061618:CDS
GAATGTGGCAACTCTATTTC+TGG 0.253750 1.2:-27581885 MS.gene061618:CDS
CATAAGTGTTTCATTAGTTA+TGG 0.313486 1.2:-27581849 MS.gene061618:CDS
TATCTTCCACAGATTATTGA+TGG 0.332558 1.2:-27581048 None:intergenic
GCACAATGTGAAGGTGATAT+TGG 0.371110 1.2:-27581960 MS.gene061618:CDS
CTTTATCAGCGATGGAAAAT+GGG 0.376842 1.2:-27582037 MS.gene061618:CDS
AATGTTGCTGGAAGTGGTTT+TGG 0.382953 1.2:-27582077 MS.gene061618:CDS
TGTCTAGATGGAACTCCATT+AGG 0.401134 1.2:+27581819 None:intergenic
AATGGGGTTGTTAGTGGTCA+TGG 0.418001 1.2:-27582020 MS.gene061618:CDS
AGTTATGGCTATTATCCTAA+TGG 0.427351 1.2:-27581834 MS.gene061618:CDS
GCAATGACAGGGCAAGATCC+AGG 0.447894 1.2:-27581251 MS.gene061618:intron
ATAATACTAGGAGGAATTGC+TGG 0.462780 1.2:-27581215 MS.gene061618:CDS
TGTGGGATAACAAATGTTGC+TGG 0.470043 1.2:-27582089 MS.gene061618:CDS
GAATATGAAGAATGTCTAGA+TGG 0.470370 1.2:+27581807 None:intergenic
ATGGAAAATGGGGTTGTTAG+TGG 0.485449 1.2:-27582026 MS.gene061618:CDS
GTTCTTACCTGAAGAAAATG+AGG 0.485674 1.2:+27581785 None:intergenic
ATAACAAATGTTGCTGGAAG+TGG 0.510378 1.2:-27582083 MS.gene061618:CDS
AAAACAGCTGCTATAATACT+AGG 0.532912 1.2:-27581227 MS.gene061618:CDS
CAGATGCTCTATAAGACTTG+TGG 0.533238 1.2:-27582107 MS.gene061618:CDS
AGATGCTCTATAAGACTTGT+GGG 0.545982 1.2:-27582106 MS.gene061618:CDS
GGACGCTGCTTTATCAGCGA+TGG 0.565779 1.2:-27582045 MS.gene061618:CDS
ACTAGGAGGAATTGCTGGAG+TGG 0.599765 1.2:-27581210 MS.gene061618:CDS
GATATTGGTGACTCTGATTG+TGG 0.607730 1.2:-27581945 MS.gene061618:CDS
CAATGACAGGGCAAGATCCA+GGG 0.608246 1.2:-27581250 MS.gene061618:intron
CAGAGAGCTCAAGTTGAATG+TGG 0.627327 1.2:-27581900 MS.gene061618:CDS
ACAGCTGCTATAATACTAGG+AGG 0.630493 1.2:-27581224 MS.gene061618:CDS
TTTATCAGCGATGGAAAATG+GGG 0.640047 1.2:-27582036 MS.gene061618:CDS
AATTTGACAAAGAAACACAA+TGG 0.727029 1.2:-27581155 MS.gene061618:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATATCAATTTAGTTCA+TGG - chr1.2:27581580-27581599 MS.gene061618:intron 15.0%
!!! TTTTTATACAACAAACTATA+TGG - chr1.2:27581173-27581192 MS.gene061618:CDS 15.0%
!! AAAATAAGTGTCAATTTGTA+TGG - chr1.2:27581458-27581477 MS.gene061618:intron 20.0%
!!! ATTTTGAAATATTGATGATG+TGG - chr1.2:27581798-27581817 MS.gene061618:CDS 20.0%
!!! TTATATTTTTGCTATTTGCT+AGG - chr1.2:27581992-27582011 MS.gene061618:CDS 20.0%
! AAATAAGTAAGTGGATGTAA+TGG - chr1.2:27581632-27581651 MS.gene061618:intron 25.0%
! AATTTGACAAAGAAACACAA+TGG - chr1.2:27582015-27582034 MS.gene061618:CDS 25.0%
! CATAAGTGTTTCATTAGTTA+TGG - chr1.2:27581321-27581340 MS.gene061618:intron 25.0%
! CTTAACCATTGGAAAATTTA+GGG + chr1.2:27581853-27581872 None:intergenic 25.0%
! TAACCAACATTTGTCATAAA+GGG + chr1.2:27581516-27581535 None:intergenic 25.0%
! TGATAATAATTTCAAACCGA+GGG - chr1.2:27581740-27581759 MS.gene061618:intron 25.0%
! TTAACCAACATTTGTCATAA+AGG + chr1.2:27581517-27581536 None:intergenic 25.0%
!! AAGATGAATTTGAAACTAAG+TGG - chr1.2:27581698-27581717 MS.gene061618:intron 25.0%
!! ATGTAATGGTTGATATTTGT+AGG - chr1.2:27581646-27581665 MS.gene061618:intron 25.0%
!! CTATATGGCATTTTATGTTT+TGG - chr1.2:27581188-27581207 MS.gene061618:CDS 25.0%
!! TTGATAATAATTTCAAACCG+AGG - chr1.2:27581739-27581758 MS.gene061618:intron 25.0%
!!! CTTATTTTTGGTGATCAATT+TGG + chr1.2:27581619-27581638 None:intergenic 25.0%
!!! TACATCCACTTACTTATTTT+TGG + chr1.2:27581631-27581650 None:intergenic 25.0%
AAAACAGCTGCTATAATACT+AGG - chr1.2:27581943-27581962 MS.gene061618:CDS 30.0%
AGTTATGGCTATTATCCTAA+TGG - chr1.2:27581336-27581355 MS.gene061618:intron 30.0%
CCTTAACCATTGGAAAATTT+AGG + chr1.2:27581854-27581873 None:intergenic 30.0%
CTAATGTTAAAACCTACAGA+TGG - chr1.2:27581407-27581426 MS.gene061618:intron 30.0%
GAATATGAAGAATGTCTAGA+TGG + chr1.2:27581366-27581385 None:intergenic 30.0%
GATCACCAAAAATAAGTAAG+TGG - chr1.2:27581623-27581642 MS.gene061618:intron 30.0%
! CCTAAATTTTCCAATGGTTA+AGG - chr1.2:27581851-27581870 MS.gene061618:CDS 30.0%
! CTTTTCCAAACACCAAATTT+TGG + chr1.2:27581435-27581454 None:intergenic 30.0%
! TCATATTCCTCATTTTCTTC+AGG - chr1.2:27581378-27581397 MS.gene061618:intron 30.0%
! TTTTCCAATGGTTAAGGAAA+AGG - chr1.2:27581857-27581876 MS.gene061618:CDS 30.0%
!! ATTGATTTGATGCAATGACA+GGG - chr1.2:27581908-27581927 MS.gene061618:CDS 30.0%
!! CAAAATTTGGTGTTTGGAAA+AGG - chr1.2:27581433-27581452 MS.gene061618:intron 30.0%
!! TATTGATTTGATGCAATGAC+AGG - chr1.2:27581907-27581926 MS.gene061618:CDS 30.0%
AACATTTGTCATAAAGGGCA+AGG + chr1.2:27581511-27581530 None:intergenic 35.0%
AGATGCTCTATAAGACTTGT+GGG - chr1.2:27581064-27581083 MS.gene061618:intron 35.0%
ATAACAAATGTTGCTGGAAG+TGG - chr1.2:27581087-27581106 MS.gene061618:intron 35.0%
ATAATACTAGGAGGAATTGC+TGG - chr1.2:27581955-27581974 MS.gene061618:CDS 35.0%
CTACAGATGGAACCAAAATT+TGG - chr1.2:27581420-27581439 MS.gene061618:intron 35.0%
CTTTATCAGCGATGGAAAAT+GGG - chr1.2:27581133-27581152 MS.gene061618:intron 35.0%
GTTCTTACCTGAAGAAAATG+AGG + chr1.2:27581388-27581407 None:intergenic 35.0%
TTGCCCTTTATGACAAATGT+TGG - chr1.2:27581510-27581529 MS.gene061618:intron 35.0%
TTTATCAGCGATGGAAAATG+GGG - chr1.2:27581134-27581153 MS.gene061618:intron 35.0%
! AGTACCTTTTCCTTAACCAT+TGG + chr1.2:27581864-27581883 None:intergenic 35.0%
! TTTTAATCGAGTTTGTCCCT+CGG + chr1.2:27581759-27581778 None:intergenic 35.0%
!! TGGAACCAAAATTTGGTGTT+TGG - chr1.2:27581427-27581446 MS.gene061618:intron 35.0%
!!! CAAATTTTGGTTCCATCTGT+AGG + chr1.2:27581422-27581441 None:intergenic 35.0%
!!! TATGTTTTGGCACAATGTGA+AGG - chr1.2:27581201-27581220 MS.gene061618:CDS 35.0%
ACAGCTGCTATAATACTAGG+AGG - chr1.2:27581946-27581965 MS.gene061618:CDS 40.0%
ATGGAAAATGGGGTTGTTAG+TGG - chr1.2:27581144-27581163 MS.gene061618:intron 40.0%
CAGATGCTCTATAAGACTTG+TGG - chr1.2:27581063-27581082 MS.gene061618:intron 40.0%
GAATGTGGCAACTCTATTTC+TGG - chr1.2:27581285-27581304 MS.gene061618:intron 40.0%
GCACAATGTGAAGGTGATAT+TGG - chr1.2:27581210-27581229 MS.gene061618:CDS 40.0%
GCTTTATCAGCGATGGAAAA+TGG - chr1.2:27581132-27581151 MS.gene061618:intron 40.0%
TGTCTAGATGGAACTCCATT+AGG + chr1.2:27581354-27581373 None:intergenic 40.0%
TGTGGGATAACAAATGTTGC+TGG - chr1.2:27581081-27581100 MS.gene061618:intron 40.0%
! AATGTTGCTGGAAGTGGTTT+TGG - chr1.2:27581093-27581112 MS.gene061618:intron 40.0%
! CTGCTCCCTAAATTTTCCAA+TGG - chr1.2:27581845-27581864 MS.gene061618:CDS 40.0%
! GATATTGGTGACTCTGATTG+TGG - chr1.2:27581225-27581244 MS.gene061618:CDS 40.0%
!! ATTATAGCAGCTGTTTTCCC+TGG + chr1.2:27581940-27581959 None:intergenic 40.0%
AATGGGGTTGTTAGTGGTCA+TGG - chr1.2:27581150-27581169 MS.gene061618:intron 45.0%
!! CAGAGAGCTCAAGTTGAATG+TGG - chr1.2:27581270-27581289 MS.gene061618:intron 45.0%
!!! AAGTGGTTTTGGCGAGAGAA+GGG - chr1.2:27581104-27581123 MS.gene061618:intron 45.0%
ACTAGGAGGAATTGCTGGAG+TGG - chr1.2:27581960-27581979 MS.gene061618:CDS 50.0%
CAATGACAGGGCAAGATCCA+GGG - chr1.2:27581920-27581939 MS.gene061618:CDS 50.0%
!!! GAAGTGGTTTTGGCGAGAGA+AGG - chr1.2:27581103-27581122 MS.gene061618:intron 50.0%
GCAATGACAGGGCAAGATCC+AGG - chr1.2:27581919-27581938 MS.gene061618:CDS 55.0%
! GGACGCTGCTTTATCAGCGA+TGG - chr1.2:27581125-27581144 MS.gene061618:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 27581051 27582141 27581051 ID=MS.gene061618
chr1.2 mRNA 27581051 27582141 27581051 ID=MS.gene061618.t1;Parent=MS.gene061618
chr1.2 exon 27581793 27582141 27581793 ID=MS.gene061618.t1.exon1;Parent=MS.gene061618.t1
chr1.2 CDS 27581793 27582141 27581793 ID=cds.MS.gene061618.t1;Parent=MS.gene061618.t1
chr1.2 exon 27581156 27581263 27581156 ID=MS.gene061618.t1.exon2;Parent=MS.gene061618.t1
chr1.2 CDS 27581156 27581263 27581156 ID=cds.MS.gene061618.t1;Parent=MS.gene061618.t1
chr1.2 exon 27581051 27581058 27581051 ID=MS.gene061618.t1.exon3;Parent=MS.gene061618.t1
chr1.2 CDS 27581051 27581058 27581051 ID=cds.MS.gene061618.t1;Parent=MS.gene061618.t1
Gene Sequence

>MS.gene061618

ATGTTAGGTATGCAGATGCTCTATAAGACTTGTGGGATAACAAATGTTGCTGGAAGTGGTTTTGGCGAGAGAAGGGACGCTGCTTTATCAGCGATGGAAAATGGGGTTGTTAGTGGTCATGGTTTTTATACAACAAACTATATGGCATTTTATGTTTTGGCACAATGTGAAGGTGATATTGGTGACTCTGATTGTGGACAGTGTGTAAAAACTGCTGTTCAGAGAGCTCAAGTTGAATGTGGCAACTCTATTTCTGGTGAAATCTTTTTGCATAAGTGTTTCATTAGTTATGGCTATTATCCTAATGGAGTTCCATCTAGACATTCTTCATATTCCTCATTTTCTTCAGGTAAGAACTAATGTTAAAACCTACAGATGGAACCAAAATTTGGTGTTTGGAAAAGGTGAAAATAAGTGTCAATTTGTATGGTAAATTTTGACTAATCTGTCATACTGTCCTTGCCCTTTATGACAAATGTTGGTTAATTTCACAATGAGTAAATATATGAACTAGTCGATAAAATTGTTGTAAAATATCAATTTAGTTCATGGCATTATAGATAGTCCAAATTGATCACCAAAAATAAGTAAGTGGATGTAATGGTTGATATTTGTAGGACTAGAATGACATTAAGTTTGTAATCTCAAAGATGAATTTGAAACTAAGTGGATGTTGTCTGCGATGCATTTGATAATAATTTCAAACCGAGGGACAAACTCGATTAAAATTTTGTAATTCTAGACATCATTTTGAAATATTGATGATGTGGAATACTAGATTGATTAAAATAATTCTGCTCCCTAAATTTTCCAATGGTTAAGGAAAAGGTACTAGTCTTCTATCTAAGTATGATCATATTGATTTGATGCAATGACAGGGCAAGATCCAGGGAAAACAGCTGCTATAATACTAGGAGGAATTGCTGGAGTGGCTTTTCTAGTTATATTTTTGCTATTTGCTAGGAATTTGACAAAGAAACACAATGGTATGTATGAGTTATTTATGAAGTCTTATGTTTATATTTTAGTCTCATGTTTTGATTCTTATTTACTCATTTTGCAATATCATTTTATCTTCCACAGATTATTGA

Protein sequence

>MS.gene061618.t1

MLGMQMLYKTCGITNVAGSGFGERRDAALSAMENGVVSGHGFYTTNYMAFYVLAQCEGDIGDSDCGQCVKTAVQRAQVECGNSISGEIFLHKCFISYGYYPNGVPSRHSSYSSFSSGQDPGKTAAIILGGIAGVAFLVIFLLFARNLTKKHNDY