AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062352


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062352.t1 MTR_5g091690 94.118 153 2 3 1 152 1 147 2.05e-97 277
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062352.t1 AT2G42870 53.704 54 25 0 99 152 65 118 4.30e-17 72.8
MS.gene062352.t1 AT3G58850 59.649 57 23 0 96 152 62 118 4.34e-17 72.8

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGAAGGTAGTGATGAT+AGG 0.324152 5.1:+73327042 MS.gene062352:CDS
GTACCTAATGGTGAGTCTTT+TGG 0.329931 5.1:+73327104 MS.gene062352:CDS
TACTGGTTTCTTTGAAAAGT+TGG 0.339174 5.1:+73327202 MS.gene062352:CDS
AAAGCTTTGAAAGCTCTTAC+TGG 0.352948 5.1:+73327185 MS.gene062352:CDS
CACCTTCTGTGCACCGTCTT+CGG 0.374930 5.1:-73326917 None:intergenic
TGAAGAAGGTAGTGATGATA+GGG 0.429741 5.1:+73327043 MS.gene062352:CDS
GAACACTACTGCTACTACTC+AGG 0.431911 5.1:+73326860 MS.gene062352:CDS
ACACCAAAAGACTCACCATT+AGG 0.468322 5.1:-73327107 None:intergenic
TGCTGTTGAGGAGAAAGAAA+AGG 0.502912 5.1:+73327007 MS.gene062352:CDS
GAGAAGGACAAGACTAAACT+TGG 0.504810 5.1:+73327224 MS.gene062352:CDS
AAGATGTTGATAAAAGAAGT+TGG 0.520911 5.1:+73326957 MS.gene062352:CDS
AGCCGAAGACGGTGCACAGA+AGG 0.521620 5.1:+73326915 MS.gene062352:CDS
TTTCTTTGAAAAGTTGGAGA+AGG 0.524494 5.1:+73327208 MS.gene062352:CDS
AGAAGGTGGTGGTGGTAGCA+AGG 0.571947 5.1:+73326932 MS.gene062352:CDS
CGGTGCACAGAAGGTGGTGG+TGG 0.573721 5.1:+73326924 MS.gene062352:CDS
CGAAGACGGTGCACAGAAGG+TGG 0.575194 5.1:+73326918 MS.gene062352:CDS
GAGTGATGATTGTGCTGTTG+AGG 0.584490 5.1:+73326995 MS.gene062352:CDS
ATCCATGGAAGAAGAAAACA+TGG 0.585509 5.1:+73326791 None:intergenic
AAGGACAAGACTAAACTTGG+AGG 0.589871 5.1:+73327227 MS.gene062352:CDS
GAAAGATTCATGCATTGCAG+AGG 0.606840 5.1:+73327078 MS.gene062352:CDS
AGACGGTGCACAGAAGGTGG+TGG 0.609872 5.1:+73326921 MS.gene062352:CDS
AACACTACTGCTACTACTCA+GGG 0.614572 5.1:+73326861 MS.gene062352:CDS
TTGCAGAGGATTGTACCTAA+TGG 0.622571 5.1:+73327092 MS.gene062352:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATGTTGATAAAAGAAGT+TGG + chr5.1:73326957-73326976 MS.gene062352:CDS 25.0%
!!! ATGTTTTTGTTGAGAAATGT+TGG - chr5.1:73326840-73326859 None:intergenic 25.0%
GAAGAAAAAGAAGATGAAGA+AGG + chr5.1:73327029-73327048 MS.gene062352:CDS 30.0%
! AAAAGTCTCTGCATGTTTTA+CGG + chr5.1:73326889-73326908 MS.gene062352:CDS 30.0%
! TTTCTTTGAAAAGTTGGAGA+AGG + chr5.1:73327208-73327227 MS.gene062352:CDS 30.0%
!! TACTGGTTTCTTTGAAAAGT+TGG + chr5.1:73327202-73327221 MS.gene062352:CDS 30.0%
ATGAAGAAGGTAGTGATGAT+AGG + chr5.1:73327042-73327061 MS.gene062352:CDS 35.0%
TGAAGAAGGTAGTGATGATA+GGG + chr5.1:73327043-73327062 MS.gene062352:CDS 35.0%
! AAGCTTTTTGATGAAACTGC+TGG + chr5.1:73327134-73327153 MS.gene062352:CDS 35.0%
!! AAAGCTTTGAAAGCTCTTAC+TGG + chr5.1:73327185-73327204 MS.gene062352:CDS 35.0%
AACACTACTGCTACTACTCA+GGG + chr5.1:73326861-73326880 MS.gene062352:CDS 40.0%
AAGGACAAGACTAAACTTGG+AGG + chr5.1:73327227-73327246 MS.gene062352:CDS 40.0%
ACACCAAAAGACTCACCATT+AGG - chr5.1:73327110-73327129 None:intergenic 40.0%
GAAAGATTCATGCATTGCAG+AGG + chr5.1:73327078-73327097 MS.gene062352:CDS 40.0%
GAGAAGGACAAGACTAAACT+TGG + chr5.1:73327224-73327243 MS.gene062352:CDS 40.0%
TGCTGTTGAGGAGAAAGAAA+AGG + chr5.1:73327007-73327026 MS.gene062352:CDS 40.0%
TTGCAGAGGATTGTACCTAA+TGG + chr5.1:73327092-73327111 MS.gene062352:CDS 40.0%
! GTACCTAATGGTGAGTCTTT+TGG + chr5.1:73327104-73327123 MS.gene062352:CDS 40.0%
! TAATGGTGAGTCTTTTGGTG+TGG + chr5.1:73327109-73327128 MS.gene062352:CDS 40.0%
GAACACTACTGCTACTACTC+AGG + chr5.1:73326860-73326879 MS.gene062352:CDS 45.0%
! GAGTGATGATTGTGCTGTTG+AGG + chr5.1:73326995-73327014 MS.gene062352:CDS 45.0%
! TTTTACGGCGAAGCCGAAGA+CGG + chr5.1:73326904-73326923 MS.gene062352:CDS 50.0%
CACCTTCTGTGCACCGTCTT+CGG - chr5.1:73326920-73326939 None:intergenic 55.0%
!! AGAAGGTGGTGGTGGTAGCA+AGG + chr5.1:73326932-73326951 MS.gene062352:CDS 55.0%
AGACGGTGCACAGAAGGTGG+TGG + chr5.1:73326921-73326940 MS.gene062352:CDS 60.0%
AGCCGAAGACGGTGCACAGA+AGG + chr5.1:73326915-73326934 MS.gene062352:CDS 60.0%
CGAAGACGGTGCACAGAAGG+TGG + chr5.1:73326918-73326937 MS.gene062352:CDS 60.0%
! CGGTGCACAGAAGGTGGTGG+TGG + chr5.1:73326924-73326943 MS.gene062352:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 73326795 73327253 73326795 ID=MS.gene062352
chr5.1 mRNA 73326795 73327253 73326795 ID=MS.gene062352.t1;Parent=MS.gene062352
chr5.1 exon 73326795 73327253 73326795 ID=MS.gene062352.t1.exon1;Parent=MS.gene062352.t1
chr5.1 CDS 73326795 73327253 73326795 ID=cds.MS.gene062352.t1;Parent=MS.gene062352.t1
Gene Sequence

>MS.gene062352

ATGGAAGAAGAAAACATGGAAGAAGAGAGAAACAGAATCATACCAACATTTCTCAACAAAAACATGAACACTACTGCTACTACTCAGGGTGTTCAAAAGTCTCTGCATGTTTTACGGCGAAGCCGAAGACGGTGCACAGAAGGTGGTGGTGGTAGCAAGGAAAAGATGTTGATAAAAGAAGTTGGTATGAATGATGTGAAGAGTGATGATTGTGCTGTTGAGGAGAAAGAAAAGGAAGAAAAAGAAGATGAAGAAGGTAGTGATGATAGGGAAGAAGTAGAGAGAAAGATTCATGCATTGCAGAGGATTGTACCTAATGGTGAGTCTTTTGGTGTGGACAAGCTTTTTGATGAAACTGCTGGCTATATTGTTGCTTTACAGTATCAAGTCAAAGCTTTGAAAGCTCTTACTGGTTTCTTTGAAAAGTTGGAGAAGGACAAGACTAAACTTGGAGGTTGA

Protein sequence

>MS.gene062352.t1

MEEENMEEERNRIIPTFLNKNMNTTATTQGVQKSLHVLRRSRRRCTEGGGGSKEKMLIKEVGMNDVKSDDCAVEEKEKEEKEDEEGSDDREEVERKIHALQRIVPNGESFGVDKLFDETAGYIVALQYQVKALKALTGFFEKLEKDKTKLGG