AlfalfaGEDB | MS.gene063674

M. sativa cultivar XinJiangDaYe / MS.gene063674

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063674.t1	XP_003612257.2	94.2	154	8	1	1	154	1	153	3.30E-68	267.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063674.t1	G7JYR8	94.2	154	8	1	1	154	1	153	2.4e-68	267.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene063674	-0.808819	2.57E-50	-1.69E-46
MS.gene049281	MS.gene063674	0.840719	7.34E-58	-1.69E-46
MS.gene049320	MS.gene063674	-0.806304	8.78E-50	-1.69E-46
MS.gene050082	MS.gene063674	0.80514	1.54E-49	-1.69E-46
MS.gene050170	MS.gene063674	-0.826494	2.63E-54	-1.69E-46
MS.gene050323	MS.gene063674	-0.805134	1.55E-49	-1.69E-46
MS.gene050496	MS.gene063674	-0.869747	2.45E-66	-1.69E-46
MS.gene050504	MS.gene063674	-0.813829	2.10E-51	-1.69E-46
MS.gene050521	MS.gene063674	0.80808	3.69E-50	-1.69E-46
MS.gene050612	MS.gene063674	-0.825711	4.04E-54	-1.69E-46
MS.gene050675	MS.gene063674	-0.801333	9.46E-49	-1.69E-46
MS.gene050699	MS.gene063674	-0.80414	2.49E-49	-1.69E-46
MS.gene050868	MS.gene063674	-0.807606	4.66E-50	-1.69E-46
MS.gene050871	MS.gene063674	-0.802262	6.10E-49	-1.69E-46
MS.gene051044	MS.gene063674	0.841003	6.18E-58	-1.69E-46
MS.gene051127	MS.gene063674	0.823529	1.32E-53	-1.69E-46
MS.gene051138	MS.gene063674	0.80073	1.26E-48	-1.69E-46
MS.gene051364	MS.gene063674	0.824683	7.07E-54	-1.69E-46
MS.gene051366	MS.gene063674	0.826978	2.02E-54	-1.69E-46
MS.gene051367	MS.gene063674	0.80555	1.26E-49	-1.69E-46
MS.gene051422	MS.gene063674	0.803719	3.05E-49	-1.69E-46
MS.gene051651	MS.gene063674	0.823437	1.39E-53	-1.69E-46
MS.gene052566	MS.gene063674	0.804141	2.49E-49	-1.69E-46
MS.gene053420	MS.gene063674	0.823217	1.56E-53	-1.69E-46
MS.gene054056	MS.gene063674	0.818068	2.38E-52	-1.69E-46
MS.gene05470	MS.gene063674	-0.813085	3.06E-51	-1.69E-46
MS.gene054954	MS.gene063674	-0.826192	3.10E-54	-1.69E-46
MS.gene054959	MS.gene063674	0.823669	1.22E-53	-1.69E-46
MS.gene055251	MS.gene063674	0.823353	1.45E-53	-1.69E-46
MS.gene055362	MS.gene063674	-0.811229	7.77E-51	-1.69E-46
MS.gene055751	MS.gene063674	-0.813897	2.03E-51	-1.69E-46
MS.gene055776	MS.gene063674	-0.823107	1.65E-53	-1.69E-46
MS.gene05589	MS.gene063674	-0.851088	1.11E-60	-1.69E-46
MS.gene055942	MS.gene063674	0.881619	2.07E-70	-1.69E-46
MS.gene056198	MS.gene063674	0.807811	4.21E-50	-1.69E-46
MS.gene056504	MS.gene063674	-0.851227	1.02E-60	-1.69E-46
MS.gene056761	MS.gene063674	0.827457	1.55E-54	-1.69E-46
MS.gene058015	MS.gene063674	0.812222	4.72E-51	-1.69E-46
MS.gene058068	MS.gene063674	0.804444	2.15E-49	-1.69E-46
MS.gene058268	MS.gene063674	0.833666	4.68E-56	-1.69E-46
MS.gene058269	MS.gene063674	0.811078	8.38E-51	-1.69E-46
MS.gene058334	MS.gene063674	0.806244	9.04E-50	-1.69E-46
MS.gene058419	MS.gene063674	-0.801014	1.10E-48	-1.69E-46
MS.gene058628	MS.gene063674	0.814603	1.42E-51	-1.69E-46
MS.gene058629	MS.gene063674	0.805361	1.39E-49	-1.69E-46
MS.gene058630	MS.gene063674	0.807772	4.29E-50	-1.69E-46
MS.gene059022	MS.gene063674	-0.838176	3.36E-57	-1.69E-46
MS.gene059677	MS.gene063674	-0.809326	2.00E-50	-1.69E-46
MS.gene059865	MS.gene063674	-0.838988	2.07E-57	-1.69E-46
MS.gene060076	MS.gene063674	0.819418	1.17E-52	-1.69E-46
MS.gene060595	MS.gene063674	0.80658	7.68E-50	-1.69E-46
MS.gene060703	MS.gene063674	-0.826137	3.20E-54	-1.69E-46
MS.gene060778	MS.gene063674	-0.818345	2.06E-52	-1.69E-46
MS.gene06077	MS.gene063674	-0.818986	1.47E-52	-1.69E-46
MS.gene060982	MS.gene063674	-0.837187	6.03E-57	-1.69E-46
MS.gene061044	MS.gene063674	0.834208	3.42E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063674.t1	MTR_5g023030	95.455	154	6	1	1	154	1	153	2.34e-104	295
MS.gene063674.t1	MTR_5g023030	95.455	154	6	1	1	154	1	153	5.19e-104	295
MS.gene063674.t1	MTR_8g074760	54.658	161	60	7	1	154	1	155	5.87e-36	122

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTCTGCTTTGGATTTCTA+AGG	0.172324	5.3:+13410936	MS.gene063674:CDS
TTAGGAGAAGTGGGTTCTTT+TGG	0.237235	5.3:-13410817	None:intergenic
TGCTTTCTTCTAGCTTCCTT+AGG	0.309418	5.3:-13410772	None:intergenic
TCACCCATGCACGAGACTTT+AGG	0.317786	5.3:-13410835	None:intergenic
AAATTGTTACATTCACCTTT+CGG	0.403917	5.3:-13411091	None:intergenic
AAGGACAAGAAGTTACATAA+AGG	0.412042	5.3:+13410886	MS.gene063674:CDS
GCTTTCTTCTAGCTTCCTTA+GGG	0.417399	5.3:-13410771	None:intergenic
AATGAAACCACATGTGTTGT+AGG	0.443488	5.3:-13410745	None:intergenic
AGGACAAGAAGTTACATAAA+GGG	0.488699	5.3:+13410887	MS.gene063674:CDS
TCTAAGGGAAGTTATGAAGC+AGG	0.489058	5.3:+13410952	MS.gene063674:CDS
TTCTCCTAAAGTCTCGTGCA+TGG	0.496688	5.3:+13410831	MS.gene063674:CDS
ATCGACAAGGACTAAGAGTT+GGG	0.514111	5.3:-13410720	None:intergenic
AAGAAATTTACCAGTGGGAG+AGG	0.523630	5.3:+13411039	MS.gene063674:CDS
GCACGAGACTTTAGGAGAAG+TGG	0.524552	5.3:-13410827	None:intergenic
TGTGGTTTCATTATTCCCTA+AGG	0.534785	5.3:+13410756	MS.gene063674:CDS
CAACCACCAGTGTCTTTAGC+GGG	0.552346	5.3:+13410688	MS.gene063674:CDS
AAGCTAGAAGAAAGCACAGA+AGG	0.555212	5.3:+13410779	MS.gene063674:CDS
TCATAGAATCCAACGTTGGA+GGG	0.555841	5.3:-13411017	None:intergenic
AGCTAGAAGAAAGCACAGAA+GGG	0.558361	5.3:+13410780	MS.gene063674:CDS
TTCATAGAATCCAACGTTGG+AGG	0.559594	5.3:-13411018	None:intergenic
GATCGACAAGGACTAAGAGT+TGG	0.560227	5.3:-13410721	None:intergenic
AGTGAATGTCCCTCCAACGT+TGG	0.563858	5.3:+13411008	MS.gene063674:CDS
CACGAGACTTTAGGAGAAGT+GGG	0.567559	5.3:-13410826	None:intergenic
TCTCCTAAAGTCTCGTGCAT+GGG	0.572514	5.3:+13410832	MS.gene063674:CDS
GTTCTGCTTTGGATTTCTAA+GGG	0.577860	5.3:+13410937	MS.gene063674:CDS
CTAAGGGAAGTTATGAAGCA+GGG	0.583061	5.3:+13410953	MS.gene063674:CDS
TGATATCCCGCTAAAGACAC+TGG	0.590824	5.3:-13410694	None:intergenic
ACAACCACCAGTGTCTTTAG+CGG	0.593632	5.3:+13410687	MS.gene063674:CDS
GAGACAAGGTGGGAAAGAGA+AGG	0.596540	5.3:+13410975	MS.gene063674:CDS
ACACAATGATCCTCTCCCAC+TGG	0.608376	5.3:-13411049	None:intergenic
AGTTATGAAGCAGGGAGACA+AGG	0.608744	5.3:+13410961	MS.gene063674:CDS
CTATGAAGAAATTTACCAGT+GGG	0.611053	5.3:+13411034	MS.gene063674:CDS
TTCTTCATAGAATCCAACGT+TGG	0.612106	5.3:-13411021	None:intergenic
TATGAAGCAGGGAGACAAGG+TGG	0.615974	5.3:+13410964	MS.gene063674:CDS
TTGATGCAACACTATCCGAA+AGG	0.634236	5.3:+13411076	MS.gene063674:CDS
GCTAGAAGAAAGCACAGAAG+GGG	0.641612	5.3:+13410781	MS.gene063674:CDS
ATGAAGCAGGGAGACAAGGT+GGG	0.641635	5.3:+13410965	MS.gene063674:CDS
TCGATCACCTACAACACATG+TGG	0.650680	5.3:+13410738	MS.gene063674:CDS
TCTATGAAGAAATTTACCAG+TGG	0.673132	5.3:+13411033	MS.gene063674:CDS
TATCCCGCTAAAGACACTGG+TGG	0.724982	5.3:-13410691	None:intergenic
TGTGTTGTAGGTGATCGACA+AGG	0.755971	5.3:-13410733	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGGACAAGAAGTTACATAA+AGG	+	chr5.3:13410886-13410905	MS.gene063674:CDS	30.0%
	AGGACAAGAAGTTACATAAA+GGG	+	chr5.3:13410887-13410906	MS.gene063674:CDS	30.0%
	CAAGAAGAAGAAGAAAAAGA+AGG	+	chr5.3:13410867-13410886	MS.gene063674:CDS	30.0%
	CTATGAAGAAATTTACCAGT+GGG	+	chr5.3:13411034-13411053	MS.gene063674:CDS	30.0%
	TCTATGAAGAAATTTACCAG+TGG	+	chr5.3:13411033-13411052	MS.gene063674:CDS	30.0%
	AATGAAACCACATGTGTTGT+AGG	-	chr5.3:13410748-13410767	None:intergenic	35.0%
	CAAACAAAAACGTTCTGCTT+TGG	+	chr5.3:13410926-13410945	MS.gene063674:CDS	35.0%
	TGTGGTTTCATTATTCCCTA+AGG	+	chr5.3:13410756-13410775	MS.gene063674:CDS	35.0%
	TTCTTCATAGAATCCAACGT+TGG	-	chr5.3:13411024-13411043	None:intergenic	35.0%
!	CAAAGCAGAACGTTTTTGTT+TGG	-	chr5.3:13410928-13410947	None:intergenic	35.0%
!	GTTCTGCTTTGGATTTCTAA+GGG	+	chr5.3:13410937-13410956	MS.gene063674:CDS	35.0%
	AAGAAATTTACCAGTGGGAG+AGG	+	chr5.3:13411039-13411058	MS.gene063674:CDS	40.0%
	ATCGACAAGGACTAAGAGTT+GGG	-	chr5.3:13410723-13410742	None:intergenic	40.0%
	CTAAGGGAAGTTATGAAGCA+GGG	+	chr5.3:13410953-13410972	MS.gene063674:CDS	40.0%
	TCATAGAATCCAACGTTGGA+GGG	-	chr5.3:13411020-13411039	None:intergenic	40.0%
	TCTAAGGGAAGTTATGAAGC+AGG	+	chr5.3:13410952-13410971	MS.gene063674:CDS	40.0%
	TTCATAGAATCCAACGTTGG+AGG	-	chr5.3:13411021-13411040	None:intergenic	40.0%
	TTGATGCAACACTATCCGAA+AGG	+	chr5.3:13411076-13411095	MS.gene063674:CDS	40.0%
!	AAGCTAGAAGAAAGCACAGA+AGG	+	chr5.3:13410779-13410798	MS.gene063674:CDS	40.0%
!	AGCTAGAAGAAAGCACAGAA+GGG	+	chr5.3:13410780-13410799	MS.gene063674:CDS	40.0%
!	CGTTCTGCTTTGGATTTCTA+AGG	+	chr5.3:13410936-13410955	MS.gene063674:CDS	40.0%
!!	GCTTTCTTCTAGCTTCCTTA+GGG	-	chr5.3:13410774-13410793	None:intergenic	40.0%
!!	TGCTTTCTTCTAGCTTCCTT+AGG	-	chr5.3:13410775-13410794	None:intergenic	40.0%
!!	TTAGGAGAAGTGGGTTCTTT+TGG	-	chr5.3:13410820-13410839	None:intergenic	40.0%
	ACAACCACCAGTGTCTTTAG+CGG	+	chr5.3:13410687-13410706	MS.gene063674:CDS	45.0%
	AGTTATGAAGCAGGGAGACA+AGG	+	chr5.3:13410961-13410980	MS.gene063674:CDS	45.0%
	GATCGACAAGGACTAAGAGT+TGG	-	chr5.3:13410724-13410743	None:intergenic	45.0%
	TCGATCACCTACAACACATG+TGG	+	chr5.3:13410738-13410757	MS.gene063674:CDS	45.0%
	TCTCCTAAAGTCTCGTGCAT+GGG	+	chr5.3:13410832-13410851	MS.gene063674:CDS	45.0%
	TGATATCCCGCTAAAGACAC+TGG	-	chr5.3:13410697-13410716	None:intergenic	45.0%
	TGTGTTGTAGGTGATCGACA+AGG	-	chr5.3:13410736-13410755	None:intergenic	45.0%
	TTCTCCTAAAGTCTCGTGCA+TGG	+	chr5.3:13410831-13410850	MS.gene063674:CDS	45.0%
!	CACGAGACTTTAGGAGAAGT+GGG	-	chr5.3:13410829-13410848	None:intergenic	45.0%
!	GCTAGAAGAAAGCACAGAAG+GGG	+	chr5.3:13410781-13410800	MS.gene063674:CDS	45.0%
	ACACAATGATCCTCTCCCAC+TGG	-	chr5.3:13411052-13411071	None:intergenic	50.0%
	AGTGAATGTCCCTCCAACGT+TGG	+	chr5.3:13411008-13411027	MS.gene063674:CDS	50.0%
	ATGAAGCAGGGAGACAAGGT+GGG	+	chr5.3:13410965-13410984	MS.gene063674:CDS	50.0%
	CAACCACCAGTGTCTTTAGC+GGG	+	chr5.3:13410688-13410707	MS.gene063674:CDS	50.0%
	GAGACAAGGTGGGAAAGAGA+AGG	+	chr5.3:13410975-13410994	MS.gene063674:CDS	50.0%
	TATCCCGCTAAAGACACTGG+TGG	-	chr5.3:13410694-13410713	None:intergenic	50.0%
	TATGAAGCAGGGAGACAAGG+TGG	+	chr5.3:13410964-13410983	MS.gene063674:CDS	50.0%
	TCACCCATGCACGAGACTTT+AGG	-	chr5.3:13410838-13410857	None:intergenic	50.0%
!	GCACGAGACTTTAGGAGAAG+TGG	-	chr5.3:13410830-13410849	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	13410637	13411101	13410637	ID=MS.gene063674
chr5.3	mRNA	13410637	13411101	13410637	ID=MS.gene063674.t1;Parent=MS.gene063674
chr5.3	exon	13410637	13411101	13410637	ID=MS.gene063674.t1.exon1;Parent=MS.gene063674.t1
chr5.3	CDS	13410637	13411101	13410637	ID=cds.MS.gene063674.t1;Parent=MS.gene063674.t1

Gene Sequence

>MS.gene063674

ATGGAGAAACTGTTTAAAATCAAAACAACGTTCTTGAAGTTTCTATCTAAACAACCACCAGTGTCTTTAGCGGGATATCAGAACCCAACTCTTAGTCCTTGTCGATCACCTACAACACATGTGGTTTCATTATTCCCTAAGGAAGCTAGAAGAAAGCACAGAAGGGGTATTAGTTTTAGTCCAAAAGAACCCACTTCTCCTAAAGTCTCGTGCATGGGTGAAATTAAGAGCAAGAAGAAGAAGAAAAAGAAGGACAAGAAGTTACATAAAGGGATTCAAGATGTTGTTCCAAACAAAAACGTTCTGCTTTGGATTTCTAAGGGAAGTTATGAAGCAGGGAGACAAGGTGGGAAAGAGAAGGAATCAGCTATAGTGAATGTCCCTCCAACGTTGGATTCTATGAAGAAATTTACCAGTGGGAGAGGATCATTGTGTGATTTTGATGCAACACTATCCGAAAGGTGA

Protein sequence

>MS.gene063674.t1

MEKLFKIKTTFLKFLSKQPPVSLAGYQNPTLSPCRSPTTHVVSLFPKEARRKHRRGISFSPKEPTSPKVSCMGEIKSKKKKKKKDKKLHKGIQDVVPNKNVLLWISKGSYEAGRQGGKEKESAIVNVPPTLDSMKKFTSGRGSLCDFDATLSER