AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068296


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068296.t1 MTR_4g013385 97.382 191 5 0 1 191 1 191 6.43e-140 388
MS.gene068296.t1 MTR_0433s0040 97.382 191 5 0 1 191 1 191 6.43e-140 388
MS.gene068296.t1 MTR_4g013355 68.947 190 48 2 3 191 1 180 3.50e-92 267
MS.gene068296.t1 MTR_4g013770 66.477 176 57 1 17 190 16 191 1.74e-82 243
MS.gene068296.t1 MTR_4g013345 60.989 182 66 3 14 190 15 196 1.83e-75 225
MS.gene068296.t1 MTR_4g013350 60.656 183 66 3 14 190 15 197 2.88e-74 222
MS.gene068296.t1 MTR_4g013320 60.870 184 64 4 15 190 16 199 1.47e-72 218
MS.gene068296.t1 MTR_7g070390 59.043 188 74 1 6 190 5 192 7.69e-72 216
MS.gene068296.t1 MTR_4g013330 59.239 184 67 4 15 190 16 199 3.11e-71 214
MS.gene068296.t1 MTR_4g013335 56.354 181 73 3 15 190 17 196 1.35e-69 210
MS.gene068296.t1 MTR_4g013325 64.138 145 51 1 47 190 55 199 3.11e-66 202
MS.gene068296.t1 MTR_4g013310 55.435 184 72 5 15 190 16 197 3.26e-65 199
MS.gene068296.t1 MTR_1g054525 46.486 185 94 3 9 190 10 192 9.93e-57 177
MS.gene068296.t1 MTR_7g093850 41.489 188 107 3 6 190 7 194 6.95e-47 152
MS.gene068296.t1 MTR_7g093790 41.989 181 99 4 13 190 14 191 1.82e-46 151
MS.gene068296.t1 MTR_4g073950 45.098 153 82 1 40 190 40 192 2.58e-46 151
MS.gene068296.t1 MTR_7g093820 39.894 188 110 3 6 190 7 194 7.84e-46 150
MS.gene068296.t1 MTR_7g093830 40.571 175 101 3 19 190 19 193 3.71e-44 145
MS.gene068296.t1 MTR_1g056370 43.709 151 84 1 40 189 35 185 3.87e-42 140
MS.gene068296.t1 MTR_7g093870 41.358 162 77 3 41 190 38 193 2.18e-39 134
MS.gene068296.t1 MTR_5g096120 37.234 188 107 5 9 190 7 189 5.59e-38 129
MS.gene068296.t1 MTR_4g078885 36.313 179 112 2 14 191 9 186 5.85e-38 129
MS.gene068296.t1 MTR_3g034030 36.667 180 112 2 13 191 8 186 6.59e-38 129
MS.gene068296.t1 MTR_3g105640 41.558 154 83 3 42 190 37 188 1.18e-37 129
MS.gene068296.t1 MTR_3g105630 37.097 186 108 5 11 190 7 189 1.76e-36 125
MS.gene068296.t1 MTR_4g122130 34.211 152 99 1 40 190 29 180 4.16e-25 96.3
MS.gene068296.t1 MTR_4g122110 34.615 156 98 2 36 190 28 180 1.96e-24 94.7
MS.gene068296.t1 MTR_4g049550 31.618 136 92 1 42 176 29 164 6.91e-16 72.4
MS.gene068296.t1 MTR_4g049570 30.496 141 92 2 46 185 33 168 2.72e-15 70.5
MS.gene068296.t1 MTR_1g046490 38.462 91 55 1 77 167 121 210 6.99e-14 67.8
MS.gene068296.t1 MTR_1g046500 39.560 91 54 1 77 167 135 224 2.87e-13 66.6
MS.gene068296.t1 MTR_1g046800 40.000 90 53 1 78 167 136 224 2.92e-13 66.6
MS.gene068296.t1 MTR_1g115510 36.975 119 68 4 78 189 235 353 3.59e-12 64.3
MS.gene068296.t1 MTR_8g099135 32.353 136 68 5 50 169 37 164 1.84e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068296.t1 AT1G55210 51.872 187 85 3 7 190 3 187 1.05e-63 195
MS.gene068296.t1 AT1G55210 51.872 187 85 3 7 190 3 187 1.05e-63 195
MS.gene068296.t1 AT1G58170 48.421 190 91 3 3 190 1 185 1.96e-63 194
MS.gene068296.t1 AT3G13650 53.801 171 76 2 21 190 18 186 3.99e-61 188
MS.gene068296.t1 AT5G49040 47.727 176 89 2 18 190 16 191 1.32e-55 174
MS.gene068296.t1 AT1G65870 45.695 151 81 1 40 189 39 189 2.55e-46 151
MS.gene068296.t1 AT5G42500 47.945 146 71 3 46 189 43 185 1.50e-44 146
MS.gene068296.t1 AT3G13660 59.829 117 47 0 74 190 9 125 2.32e-44 144
MS.gene068296.t1 AT5G42510 46.000 150 78 2 41 189 35 182 5.23e-44 145
MS.gene068296.t1 AT5G49030 45.614 171 88 3 18 185 16 184 6.07e-43 153
MS.gene068296.t1 AT3G13662 47.445 137 70 1 41 177 38 172 1.44e-41 138
MS.gene068296.t1 AT1G22900 44.667 150 80 2 41 189 46 193 5.18e-40 135
MS.gene068296.t1 AT2G21100 40.940 149 87 1 42 189 39 187 6.85e-39 132
MS.gene068296.t1 AT2G21100 40.940 149 87 1 42 189 39 187 6.85e-39 132
MS.gene068296.t1 AT3G58090 47.706 109 57 0 81 189 163 271 4.34e-34 122
MS.gene068296.t1 AT2G21110 37.748 151 89 1 45 190 36 186 3.42e-32 114
MS.gene068296.t1 AT4G38700 36.842 190 107 6 8 190 7 190 5.37e-31 112
MS.gene068296.t1 AT4G23690 32.847 137 86 4 37 169 32 166 1.30e-15 71.6
MS.gene068296.t1 AT4G11210 28.994 169 108 6 5 169 2 162 1.25e-13 66.2
MS.gene068296.t1 AT4G11190 30.370 135 88 3 39 169 30 162 2.03e-13 65.9
MS.gene068296.t1 AT4G11180 33.094 139 86 5 36 169 28 164 5.32e-12 62.0

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTTCTTCTGAACAATTT+AGG 0.169887 4.4:-86659272 None:intergenic
ACCTCAATTGAGCTCAAAAT+TGG 0.260472 4.4:+86659439 MS.gene068296:CDS
TGGAAGCACAATTACTATCT+TGG 0.342636 4.4:+86659556 MS.gene068296:CDS
TTATTTGAAGGGAAATATAA+TGG 0.360119 4.4:+86659536 MS.gene068296:CDS
TTAGCCACTTTAGATTCTAT+TGG 0.363260 4.4:+86659300 MS.gene068296:CDS
AAGCTCAGTTTGTGCAGTAT+AGG 0.391171 4.4:-86659486 None:intergenic
ATCTATTGAGCCCACGAAAC+CGG 0.426314 4.4:-86659249 None:intergenic
GGAAGCACAATTACTATCTT+GGG 0.437728 4.4:+86659557 MS.gene068296:CDS
GGGTGTAACAAGAGAGGAGA+AGG 0.466269 4.4:-86659199 None:intergenic
ATGCAAAATTCATTATCATA+AGG 0.474182 4.4:-86659514 None:intergenic
AAGAAATGATGCTTATGAAA+AGG 0.476165 4.4:+86659580 MS.gene068296:CDS
AAATTGGTTGGAAGGGCTCA+AGG 0.505137 4.4:+86659455 MS.gene068296:CDS
ATGCCTGTGATTGGTGGAAG+TGG 0.512180 4.4:+86659611 MS.gene068296:CDS
ATAGACAACGCTTTGACCTC+AGG 0.512795 4.4:+86659416 MS.gene068296:CDS
TGAGCTCAAAATTGGTTGGA+AGG 0.519356 4.4:+86659447 MS.gene068296:CDS
CAATTGAGCTCAAAATTGGT+TGG 0.529085 4.4:+86659443 MS.gene068296:CDS
GTTGTGTTAAACTTCGGAAG+AGG 0.543099 4.4:-86659371 None:intergenic
CATGCCAATAGAATCTAAAG+TGG 0.545838 4.4:-86659304 None:intergenic
ACAAGATGAGACCGGTTTCG+TGG 0.547240 4.4:+86659238 MS.gene068296:CDS
GTTAGAGAAATGCCTGTGAT+TGG 0.553503 4.4:+86659602 MS.gene068296:CDS
GTGTTAAACTTCGGAAGAGG+TGG 0.556530 4.4:-86659368 None:intergenic
AAGTTTAACACAACTAACTT+CGG 0.561635 4.4:+86659380 MS.gene068296:CDS
AAGTTAGTTGTGTTAAACTT+CGG 0.577434 4.4:-86659377 None:intergenic
GAGCTCAAAATTGGTTGGAA+GGG 0.580534 4.4:+86659448 MS.gene068296:CDS
CAAGATGAGACCGGTTTCGT+GGG 0.584549 4.4:+86659239 MS.gene068296:CDS
AGTCCACTTCCACCAATCAC+AGG 0.592137 4.4:-86659614 None:intergenic
TGAGCTCAATTGAGGTCCTG+AGG 0.592935 4.4:-86659432 None:intergenic
AGAGAAATGCCTGTGATTGG+TGG 0.594056 4.4:+86659605 MS.gene068296:CDS
AATTGGTTGGAAGGGCTCAA+GGG 0.595873 4.4:+86659456 MS.gene068296:CDS
CTTGTGCTGTGACGATTGTG+AGG 0.604169 4.4:-86659220 None:intergenic
AGTTGTAGCATCTCCTGTAG+TGG 0.622414 4.4:-86659687 None:intergenic
GTCACAGCACAAGATGAGAC+CGG 0.639527 4.4:+86659230 MS.gene068296:CDS
TTGTGAGGGTGTAACAAGAG+AGG 0.652546 4.4:-86659205 None:intergenic
TATTGGCATGACGTAGTCAG+CGG 0.668999 4.4:+86659317 MS.gene068296:CDS
AGGTGGAACAATTTCTATCG+CGG 0.671876 4.4:-86659351 None:intergenic
TAACACAACTAACTTCGGTG+AGG 0.681754 4.4:+86659385 MS.gene068296:CDS
TGGAACAATTTCTATCGCGG+TGG 0.688468 4.4:-86659348 None:intergenic
TTGTGCTGTGACGATTGTGA+GGG 0.694834 4.4:-86659219 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCAAAATTCATTATCATA+AGG - chr4.4:86659517-86659536 None:intergenic 20.0%
!! TTATTTGAAGGGAAATATAA+TGG + chr4.4:86659536-86659555 MS.gene068296:CDS 20.0%
!!! ATGAATTTTGCATTATTTGA+AGG + chr4.4:86659524-86659543 MS.gene068296:CDS 20.0%
!!! TGAATTTTGCATTATTTGAA+GGG + chr4.4:86659525-86659544 MS.gene068296:CDS 20.0%
! AAGAAATGATGCTTATGAAA+AGG + chr4.4:86659580-86659599 MS.gene068296:CDS 25.0%
! AAGTTAGTTGTGTTAAACTT+CGG - chr4.4:86659380-86659399 None:intergenic 25.0%
! AAGTTTAACACAACTAACTT+CGG + chr4.4:86659380-86659399 MS.gene068296:CDS 25.0%
TGTCTTCTTCTGAACAATTT+AGG - chr4.4:86659275-86659294 None:intergenic 30.0%
! TTAGCCACTTTAGATTCTAT+TGG + chr4.4:86659300-86659319 MS.gene068296:CDS 30.0%
!! TACTTTTTGAATACCACTAC+AGG + chr4.4:86659674-86659693 MS.gene068296:CDS 30.0%
!!! GGACTTTTTAGATTTGCTAA+GGG + chr4.4:86659632-86659651 MS.gene068296:CDS 30.0%
!!! TGGACTTTTTAGATTTGCTA+AGG + chr4.4:86659631-86659650 MS.gene068296:CDS 30.0%
ACCTCAATTGAGCTCAAAAT+TGG + chr4.4:86659439-86659458 MS.gene068296:CDS 35.0%
CAATTGAGCTCAAAATTGGT+TGG + chr4.4:86659443-86659462 MS.gene068296:CDS 35.0%
CATGCCAATAGAATCTAAAG+TGG - chr4.4:86659307-86659326 None:intergenic 35.0%
! ACCAATTTTGAGCTCAATTG+AGG - chr4.4:86659443-86659462 None:intergenic 35.0%
! GGAAGCACAATTACTATCTT+GGG + chr4.4:86659557-86659576 MS.gene068296:CDS 35.0%
! TGGAAGCACAATTACTATCT+TGG + chr4.4:86659556-86659575 MS.gene068296:CDS 35.0%
AAGCTCAGTTTGTGCAGTAT+AGG - chr4.4:86659489-86659508 None:intergenic 40.0%
AGGTGGAACAATTTCTATCG+CGG - chr4.4:86659354-86659373 None:intergenic 40.0%
GAGCTCAAAATTGGTTGGAA+GGG + chr4.4:86659448-86659467 MS.gene068296:CDS 40.0%
GTTAGAGAAATGCCTGTGAT+TGG + chr4.4:86659602-86659621 MS.gene068296:CDS 40.0%
GTTGTGTTAAACTTCGGAAG+AGG - chr4.4:86659374-86659393 None:intergenic 40.0%
TAACACAACTAACTTCGGTG+AGG + chr4.4:86659385-86659404 MS.gene068296:CDS 40.0%
TGAGCTCAAAATTGGTTGGA+AGG + chr4.4:86659447-86659466 MS.gene068296:CDS 40.0%
AAATTGGTTGGAAGGGCTCA+AGG + chr4.4:86659455-86659474 MS.gene068296:CDS 45.0%
AATTGGTTGGAAGGGCTCAA+GGG + chr4.4:86659456-86659475 MS.gene068296:CDS 45.0%
AGAGAAATGCCTGTGATTGG+TGG + chr4.4:86659605-86659624 MS.gene068296:CDS 45.0%
AGTTGTAGCATCTCCTGTAG+TGG - chr4.4:86659690-86659709 None:intergenic 45.0%
ATAGACAACGCTTTGACCTC+AGG + chr4.4:86659416-86659435 MS.gene068296:CDS 45.0%
ATCTATTGAGCCCACGAAAC+CGG - chr4.4:86659252-86659271 None:intergenic 45.0%
GTGTTAAACTTCGGAAGAGG+TGG - chr4.4:86659371-86659390 None:intergenic 45.0%
TATTGGCATGACGTAGTCAG+CGG + chr4.4:86659317-86659336 MS.gene068296:CDS 45.0%
TGGAACAATTTCTATCGCGG+TGG - chr4.4:86659351-86659370 None:intergenic 45.0%
TTGTGCTGTGACGATTGTGA+GGG - chr4.4:86659222-86659241 None:intergenic 45.0%
! TTGTGAGGGTGTAACAAGAG+AGG - chr4.4:86659208-86659227 None:intergenic 45.0%
AGTCCACTTCCACCAATCAC+AGG - chr4.4:86659617-86659636 None:intergenic 50.0%
ATGCCTGTGATTGGTGGAAG+TGG + chr4.4:86659611-86659630 MS.gene068296:CDS 50.0%
CTTGTGCTGTGACGATTGTG+AGG - chr4.4:86659223-86659242 None:intergenic 50.0%
GGGTGTAACAAGAGAGGAGA+AGG - chr4.4:86659202-86659221 None:intergenic 50.0%
GTCACAGCACAAGATGAGAC+CGG + chr4.4:86659230-86659249 MS.gene068296:CDS 50.0%
TGAGCTCAATTGAGGTCCTG+AGG - chr4.4:86659435-86659454 None:intergenic 50.0%
! ACAAGATGAGACCGGTTTCG+TGG + chr4.4:86659238-86659257 MS.gene068296:CDS 50.0%
! CAAGATGAGACCGGTTTCGT+GGG + chr4.4:86659239-86659258 MS.gene068296:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 86659167 86659742 86659167 ID=MS.gene068296
chr4.4 mRNA 86659167 86659742 86659167 ID=MS.gene068296.t1;Parent=MS.gene068296
chr4.4 exon 86659167 86659742 86659167 ID=MS.gene068296.t1.exon1;Parent=MS.gene068296.t1
chr4.4 CDS 86659167 86659742 86659167 ID=cds.MS.gene068296.t1;Parent=MS.gene068296.t1
Gene Sequence

>MS.gene068296

ATGGCTATAGGTGCTTTCAAAATCTTCTTCTTCCTTCTCCTCTCTTGTTACACCCTCACAATCGTCACAGCACAAGATGAGACCGGTTTCGTGGGCTCAATAGATCCTAAATTGTTCAGAAGAAGACAAAATGTTAGCCACTTTAGATTCTATTGGCATGACGTAGTCAGCGGAGACAACGCCACCGCGATAGAAATTGTTCCACCTCTTCCGAAGTTTAACACAACTAACTTCGGTGAGGTTAAAGTCATAGACAACGCTTTGACCTCAGGACCTCAATTGAGCTCAAAATTGGTTGGAAGGGCTCAAGGGATATATGCCTATACTGCACAAACTGAGCTTAATTTCCTTATGATAATGAATTTTGCATTATTTGAAGGGAAATATAATGGAAGCACAATTACTATCTTGGGAAGAAATGATGCTTATGAAAAGGTTAGAGAAATGCCTGTGATTGGTGGAAGTGGACTTTTTAGATTTGCTAAGGGATATGCTGAACTTACTACTTACTTTTTGAATACCACTACAGGAGATGCTACAACTGAGTACAATATTTATGTTTCTCATTATGTTTAA

Protein sequence

>MS.gene068296.t1

MAIGAFKIFFFLLLSCYTLTIVTAQDETGFVGSIDPKLFRRRQNVSHFRFYWHDVVSGDNATAIEIVPPLPKFNTTNFGEVKVIDNALTSGPQLSSKLVGRAQGIYAYTAQTELNFLMIMNFALFEGKYNGSTITILGRNDAYEKVREMPVIGGSGLFRFAKGYAELTTYFLNTTTGDATTEYNIYVSHYV