AlfalfaGEDB | MS.gene068297

M. sativa cultivar XinJiangDaYe / MS.gene068297

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068297.t1	XP_003604505.2	97.4	192	5	0	1	192	76	267	1.40E-100	375.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068297.t1	Q9C523	54.0	187	73	4	10	191	7	185	3.1e-48	193.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068297.t1	G7JKJ6	97.4	192	5	0	1	192	1	192	1.0e-100	375.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049147	MS.gene068297	0.80261	5.17E-49	-1.69E-46
MS.gene049267	MS.gene068297	0.817339	3.47E-52	-1.69E-46
MS.gene049611	MS.gene068297	0.81672	4.78E-52	-1.69E-46
MS.gene049634	MS.gene068297	0.805525	1.28E-49	-1.69E-46
MS.gene050380	MS.gene068297	0.864524	1.14E-64	-1.69E-46
MS.gene050381	MS.gene068297	0.850534	1.60E-60	-1.69E-46
MS.gene050809	MS.gene068297	0.848776	4.94E-60	-1.69E-46
MS.gene05104	MS.gene068297	0.838478	2.81E-57	-1.69E-46
MS.gene051127	MS.gene068297	0.812148	4.90E-51	-1.69E-46
MS.gene051632	MS.gene068297	0.819595	1.07E-52	-1.69E-46
MS.gene051863	MS.gene068297	0.811361	7.27E-51	-1.69E-46
MS.gene052805	MS.gene068297	0.805516	1.29E-49	-1.69E-46
MS.gene053420	MS.gene068297	0.81431	1.64E-51	-1.69E-46
MS.gene053488	MS.gene068297	0.815856	7.46E-52	-1.69E-46
MS.gene053561	MS.gene068297	0.807822	4.19E-50	-1.69E-46
MS.gene054290	MS.gene068297	0.832023	1.20E-55	-1.69E-46
MS.gene054742	MS.gene068297	0.80876	2.64E-50	-1.69E-46
MS.gene055124	MS.gene068297	0.883195	5.51E-71	-1.69E-46
MS.gene055154	MS.gene068297	0.817949	2.53E-52	-1.69E-46
MS.gene055202	MS.gene068297	0.801195	1.01E-48	-1.69E-46
MS.gene055959	MS.gene068297	0.89909	2.79E-77	-1.69E-46
MS.gene056313	MS.gene068297	0.840451	8.63E-58	-1.69E-46
MS.gene056330	MS.gene068297	0.80256	5.29E-49	-1.69E-46
MS.gene056422	MS.gene068297	-0.808303	3.31E-50	-1.69E-46
MS.gene056920	MS.gene068297	0.818064	2.38E-52	-1.69E-46
MS.gene056923	MS.gene068297	0.802053	6.73E-49	-1.69E-46
MS.gene056924	MS.gene068297	0.80062	1.32E-48	-1.69E-46
MS.gene057544	MS.gene068297	0.822984	1.77E-53	-1.69E-46
MS.gene058093	MS.gene068297	0.905588	3.66E-80	-1.69E-46
MS.gene058099	MS.gene068297	0.866656	2.43E-65	-1.69E-46
MS.gene058108	MS.gene068297	0.850213	1.96E-60	-1.69E-46
MS.gene058109	MS.gene068297	0.839927	1.18E-57	-1.69E-46
MS.gene058171	MS.gene068297	0.837468	5.11E-57	-1.69E-46
MS.gene058173	MS.gene068297	0.834331	3.19E-56	-1.69E-46
MS.gene058175	MS.gene068297	0.840157	1.03E-57	-1.69E-46
MS.gene058176	MS.gene068297	0.897212	1.75E-76	-1.69E-46
MS.gene058179	MS.gene068297	0.916846	1.09E-85	-1.69E-46
MS.gene058180	MS.gene068297	0.887205	1.75E-72	-1.69E-46
MS.gene058840	MS.gene068297	0.854988	8.53E-62	-1.69E-46
MS.gene059037	MS.gene068297	0.916684	1.32E-85	-1.69E-46
MS.gene059038	MS.gene068297	0.882787	7.77E-71	-1.69E-46
MS.gene059044	MS.gene068297	0.858343	8.79E-63	-1.69E-46
MS.gene059045	MS.gene068297	0.843895	1.06E-58	-1.69E-46
MS.gene059223	MS.gene068297	0.819376	1.20E-52	-1.69E-46
MS.gene059367	MS.gene068297	-0.803946	2.73E-49	-1.69E-46
MS.gene059682	MS.gene068297	0.826121	3.23E-54	-1.69E-46
MS.gene059944	MS.gene068297	0.811326	7.40E-51	-1.69E-46
MS.gene060205	MS.gene068297	0.843574	1.29E-58	-1.69E-46
MS.gene060578	MS.gene068297	0.837899	3.96E-57	-1.69E-46
MS.gene06074	MS.gene068297	0.847146	1.39E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068297.t1	MTR_4g013770	97.396	192	5	0	1	192	1	192	3.06e-137	381
MS.gene068297.t1	MTR_4g013385	68.817	186	56	1	6	191	7	190	2.28e-89	260
MS.gene068297.t1	MTR_0433s0040	68.817	186	56	1	6	191	7	190	2.28e-89	260
MS.gene068297.t1	MTR_4g013355	66.667	186	51	2	6	191	5	179	6.16e-84	246
MS.gene068297.t1	MTR_4g013350	61.413	184	65	3	13	191	15	197	1.07e-70	213
MS.gene068297.t1	MTR_4g013345	60.109	183	68	3	13	191	15	196	2.80e-68	207
MS.gene068297.t1	MTR_4g013330	59.218	179	69	3	17	191	21	199	2.95e-68	207
MS.gene068297.t1	MTR_4g013335	61.765	170	60	2	24	191	30	196	6.78e-68	206
MS.gene068297.t1	MTR_7g070390	57.447	188	75	2	7	191	7	192	1.09e-66	202
MS.gene068297.t1	MTR_4g013320	56.497	177	74	2	17	191	24	199	5.23e-65	199
MS.gene068297.t1	MTR_4g013325	63.699	146	52	1	46	191	55	199	3.89e-64	196
MS.gene068297.t1	MTR_4g013310	57.143	168	66	4	24	188	30	194	4.65e-62	191
MS.gene068297.t1	MTR_1g054525	47.668	193	91	5	6	191	3	192	4.86e-54	171
MS.gene068297.t1	MTR_7g093790	42.077	183	99	5	11	191	14	191	3.14e-47	153
MS.gene068297.t1	MTR_7g093850	40.909	176	101	3	18	191	20	194	1.70e-44	146
MS.gene068297.t1	MTR_7g093820	39.773	176	103	3	18	191	20	194	1.05e-43	144
MS.gene068297.t1	MTR_4g073950	42.105	152	88	0	40	191	41	192	6.99e-43	142
MS.gene068297.t1	MTR_7g093830	38.068	176	106	3	18	191	19	193	1.46e-42	141
MS.gene068297.t1	MTR_1g056370	45.395	152	80	3	40	190	36	185	2.89e-42	140
MS.gene068297.t1	MTR_7g093870	42.857	168	91	3	29	191	26	193	2.87e-40	136
MS.gene068297.t1	MTR_3g105640	40.373	161	93	2	32	191	30	188	1.10e-34	121
MS.gene068297.t1	MTR_5g096120	39.267	191	102	5	7	191	7	189	3.75e-33	117
MS.gene068297.t1	MTR_3g105630	36.269	193	112	4	4	191	3	189	3.79e-33	117
MS.gene068297.t1	MTR_4g078885	36.735	147	90	1	45	191	42	185	7.63e-30	108
MS.gene068297.t1	MTR_3g034030	34.254	181	115	2	12	191	8	185	9.47e-30	108
MS.gene068297.t1	MTR_4g049550	34.483	145	88	2	45	186	33	173	1.46e-22	89.7
MS.gene068297.t1	MTR_4g122130	37.500	144	87	2	49	191	39	180	3.36e-22	89.0
MS.gene068297.t1	MTR_4g049570	33.793	145	89	2	45	186	33	173	1.83e-21	87.0
MS.gene068297.t1	MTR_4g122110	32.515	163	106	2	29	191	22	180	5.66e-21	85.5
MS.gene068297.t1	MTR_8g099135	32.298	161	83	5	30	174	18	168	1.24e-13	66.2
MS.gene068297.t1	MTR_1g046490	38.298	94	57	1	78	171	121	213	7.20e-13	65.1
MS.gene068297.t1	MTR_1g046800	39.362	94	56	1	78	171	135	227	2.44e-12	63.9
MS.gene068297.t1	MTR_1g046500	39.362	94	56	1	78	171	135	227	2.64e-12	63.9
MS.gene068297.t1	MTR_8g099115	33.333	135	78	4	49	174	41	172	1.75e-11	60.8
MS.gene068297.t1	MTR_8g073770	26.056	142	103	1	46	187	42	181	8.95e-11	58.9
MS.gene068297.t1	MTR_8g073850	26.056	142	103	1	46	187	42	181	9.81e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068297.t1	AT1G58170	53.476	187	74	4	10	191	7	185	5.24e-65	198
MS.gene068297.t1	AT1G55210	51.323	189	85	5	6	191	3	187	3.90e-57	178
MS.gene068297.t1	AT1G55210	51.323	189	85	5	6	191	3	187	3.90e-57	178
MS.gene068297.t1	AT5G49040	50.847	177	84	2	17	191	16	191	8.76e-56	175
MS.gene068297.t1	AT3G13650	51.445	173	79	4	20	191	18	186	7.76e-53	167
MS.gene068297.t1	AT3G13662	49.425	174	78	5	7	178	7	172	5.10e-47	152
MS.gene068297.t1	AT1G65870	47.260	146	76	1	45	190	45	189	2.85e-45	148
MS.gene068297.t1	AT5G49030	50.602	166	79	2	17	180	16	180	3.37e-44	157
MS.gene068297.t1	AT3G13660	57.143	119	51	0	73	191	7	125	2.54e-42	139
MS.gene068297.t1	AT5G42500	41.818	165	93	2	26	190	24	185	1.74e-40	136
MS.gene068297.t1	AT1G22900	46.575	146	75	2	45	190	51	193	4.24e-39	132
MS.gene068297.t1	AT5G42510	41.875	160	90	2	31	190	26	182	4.89e-39	132
MS.gene068297.t1	AT2G21100	41.447	152	88	1	39	190	37	187	6.37e-38	129
MS.gene068297.t1	AT2G21100	41.447	152	88	1	39	190	37	187	6.37e-38	129
MS.gene068297.t1	AT3G58090	46.364	110	59	0	81	190	162	271	2.10e-32	117
MS.gene068297.t1	AT4G38700	34.694	196	117	5	1	191	1	190	1.93e-28	105
MS.gene068297.t1	AT2G21110	32.432	185	116	4	11	191	7	186	5.56e-28	103
MS.gene068297.t1	AT4G23690	36.029	136	77	5	36	166	32	162	2.19e-15	71.2
MS.gene068297.t1	AT4G11210	31.579	171	103	6	12	178	7	167	4.08e-14	67.8
MS.gene068297.t1	AT4G11190	30.935	139	85	4	44	178	36	167	1.39e-12	63.5

Find 48 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTAAAAGAGTTGTTAAATT+TGG	0.090030	4.4:-86645995	None:intergenic
AAGTTTGGTGTCAAGGTTAA+AGG	0.229203	4.4:-86646048	None:intergenic
AAGTAGCTGCTAGCCACTTT+AGG	0.300872	4.4:+86645908	MS.gene068297:CDS
CTAAAAGAGTTGTTAAATTT+GGG	0.308915	4.4:-86645994	None:intergenic
TTGGGAAGTGGTTGAATGAT+TGG	0.327500	4.4:-86645976	None:intergenic
TTGTTTGAAGGAAAGTATAA+TGG	0.337558	4.4:+86646159	MS.gene068297:CDS
GGGTGTAGAAAGAGAGTAGA+AGG	0.394648	4.4:-86645816	None:intergenic
GGGAGTACCATTACTATCTC+TGG	0.407901	4.4:+86646180	MS.gene068297:CDS
ATGAACTTTGCCTTGTTTGA+AGG	0.417944	4.4:+86646147	MS.gene068297:CDS
TGTTTGAAGGAAAGTATAAT+GGG	0.433452	4.4:+86646160	MS.gene068297:CDS
CAGCATATCCCTTAGCAAAT+CGG	0.436333	4.4:-86646263	None:intergenic
ATTTATTGAGCTTACAAAAC+CGG	0.438981	4.4:-86645866	None:intergenic
AGTTTGGTGTCAAGGTTAAA+GGG	0.445162	4.4:-86646047	None:intergenic
TACCAGTGATCGGTGGTAGC+GGG	0.446390	4.4:+86646235	MS.gene068297:CDS
CTAGCCACTTTAGGTTCTAC+TGG	0.448964	4.4:+86645917	MS.gene068297:CDS
AACTTGACCTCAAAATTGTT+AGG	0.452723	4.4:+86646066	MS.gene068297:CDS
TACTTTCTTTGTAAGCAACT+TGG	0.452780	4.4:-86645890	None:intergenic
AAGGAACGTCGCAAACGATA+AGG	0.481824	4.4:+86646203	MS.gene068297:CDS
ACTTTCTTTGTAAGCAACTT+GGG	0.483229	4.4:-86645889	None:intergenic
TGACCTCAAAATTGTTAGGT+AGG	0.497868	4.4:+86646070	MS.gene068297:CDS
TACACCCTCACAATTGTCAC+TGG	0.498118	4.4:+86645832	MS.gene068297:CDS
AAATTGTTAGGTAGGGCTCA+AGG	0.500966	4.4:+86646078	MS.gene068297:CDS
TGAGGTCAAGTTTGGTGTCA+AGG	0.506209	4.4:-86646055	None:intergenic
GAGTTGTTAAATTTGGGAAG+TGG	0.508093	4.4:-86645988	None:intergenic
GTTCTACTGGCAAGACATAG+TGG	0.508556	4.4:+86645930	MS.gene068297:CDS
ACCCCGCTACCACCGATCAC+TGG	0.532338	4.4:-86646237	None:intergenic
TTACCAGTGATCGGTGGTAG+CGG	0.542224	4.4:+86646234	MS.gene068297:CDS
AATTGTTAGGTAGGGCTCAA+GGG	0.543701	4.4:+86646079	MS.gene068297:CDS
TGGTTGAATGATTGGAATGG+AGG	0.546721	4.4:-86645968	None:intergenic
GTATTCAACCACGGTATCCC+CGG	0.549581	4.4:-86646316	None:intergenic
CTTGCCAGTAGAACCTAAAG+TGG	0.555578	4.4:-86645921	None:intergenic
GACCTCAAAATTGTTAGGTA+GGG	0.562175	4.4:+86646071	MS.gene068297:CDS
ACCAGTGATCGGTGGTAGCG+GGG	0.577667	4.4:+86646236	MS.gene068297:CDS
GTCACTGGACAAGATGAGAC+CGG	0.584277	4.4:+86645847	MS.gene068297:CDS
AAGTGGTTGAATGATTGGAA+TGG	0.586361	4.4:-86645971	None:intergenic
ATTATACTTTCCTTCAAACA+AGG	0.587601	4.4:-86646157	None:intergenic
AACCATAAGAAAATCAAGCT+CGG	0.592742	4.4:-86646124	None:intergenic
GTTAGAGAGTTACCAGTGAT+CGG	0.602234	4.4:+86646225	MS.gene068297:CDS
TTCTACTGGCAAGACATAGT+GGG	0.624056	4.4:+86645931	MS.gene068297:CDS
TTTCATAACCGGGGATACCG+TGG	0.641517	4.4:+86646308	MS.gene068297:CDS
GTACCATTACTATCTCTGGA+AGG	0.647934	4.4:+86646184	MS.gene068297:CDS
ATAAACATTGTATTCAACCA+CGG	0.650255	4.4:-86646325	None:intergenic
GTTCCTTCCAGAGATAGTAA+TGG	0.662479	4.4:-86646187	None:intergenic
AAGCTCGGTTTGCGATGTAG+CGG	0.665030	4.4:-86646109	None:intergenic
TACTGGCAAGACATAGTGGG+AGG	0.669570	4.4:+86645934	MS.gene068297:CDS
CTTGTCCAGTGACAATTGTG+AGG	0.674513	4.4:-86645837	None:intergenic
TTGTCCAGTGACAATTGTGA+GGG	0.677874	4.4:-86645836	None:intergenic
AGAGAGTTACCAGTGATCGG+TGG	0.703961	4.4:+86646228	MS.gene068297:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAAAAGAGTTGTTAAATT+TGG	-	chr4.4:86645998-86646017	None:intergenic	20.0%
!!	CTAAAAGAGTTGTTAAATTT+GGG	-	chr4.4:86645997-86646016	None:intergenic	20.0%
!!!	AAGGAAGAAAAAAAGTTTTT+GGG	-	chr4.4:86645800-86645819	None:intergenic	20.0%
!	ATAAACATTGTATTCAACCA+CGG	-	chr4.4:86646328-86646347	None:intergenic	25.0%
!	ATTATACTTTCCTTCAAACA+AGG	-	chr4.4:86646160-86646179	None:intergenic	25.0%
!	ATTTATTGAGCTTACAAAAC+CGG	-	chr4.4:86645869-86645888	None:intergenic	25.0%
!!	GAAGGAAGAAAAAAAGTTTT+TGG	-	chr4.4:86645801-86645820	None:intergenic	25.0%
!!	TGTTTGAAGGAAAGTATAAT+GGG	+	chr4.4:86646160-86646179	MS.gene068297:CDS	25.0%
!!	TTGTTTGAAGGAAAGTATAA+TGG	+	chr4.4:86646159-86646178	MS.gene068297:CDS	25.0%
!!!	ATCTCTTTTGATTTCATAAC+CGG	+	chr4.4:86646297-86646316	MS.gene068297:CDS	25.0%
	AACCATAAGAAAATCAAGCT+CGG	-	chr4.4:86646127-86646146	None:intergenic	30.0%
	AACTTGACCTCAAAATTGTT+AGG	+	chr4.4:86646066-86646085	MS.gene068297:CDS	30.0%
	ACTTTCTTTGTAAGCAACTT+GGG	-	chr4.4:86645892-86645911	None:intergenic	30.0%
	TACTTTCTTTGTAAGCAACT+TGG	-	chr4.4:86645893-86645912	None:intergenic	30.0%
!	AACAACTCTTTTAGTGCTTT+TGG	+	chr4.4:86646003-86646022	MS.gene068297:CDS	30.0%
!!!	AACAATTTTGAGGTCAAGTT+TGG	-	chr4.4:86646066-86646085	None:intergenic	30.0%
!!!	TCTCTTTTGATTTCATAACC+GGG	+	chr4.4:86646298-86646317	MS.gene068297:CDS	30.0%
	AAGTGGTTGAATGATTGGAA+TGG	-	chr4.4:86645974-86645993	None:intergenic	35.0%
	GACCTCAAAATTGTTAGGTA+GGG	+	chr4.4:86646071-86646090	MS.gene068297:CDS	35.0%
	GAGTTGTTAAATTTGGGAAG+TGG	-	chr4.4:86645991-86646010	None:intergenic	35.0%
	TGACCTCAAAATTGTTAGGT+AGG	+	chr4.4:86646070-86646089	MS.gene068297:CDS	35.0%
!	AACCGAGCTTGATTTTCTTA+TGG	+	chr4.4:86646122-86646141	MS.gene068297:CDS	35.0%
!!	AAGTTTGGTGTCAAGGTTAA+AGG	-	chr4.4:86646051-86646070	None:intergenic	35.0%
!!	AGTTTGGTGTCAAGGTTAAA+GGG	-	chr4.4:86646050-86646069	None:intergenic	35.0%
!!	ATGAACTTTGCCTTGTTTGA+AGG	+	chr4.4:86646147-86646166	MS.gene068297:CDS	35.0%
!!	CTCTTTTAGTGCTTTTGGTT+TGG	+	chr4.4:86646008-86646027	MS.gene068297:CDS	35.0%
!!!	CTCTTTTGATTTCATAACCG+GGG	+	chr4.4:86646299-86646318	MS.gene068297:CDS	35.0%
	AAATTGTTAGGTAGGGCTCA+AGG	+	chr4.4:86646078-86646097	MS.gene068297:CDS	40.0%
	AATTGTTAGGTAGGGCTCAA+GGG	+	chr4.4:86646079-86646098	MS.gene068297:CDS	40.0%
	CAGCATATCCCTTAGCAAAT+CGG	-	chr4.4:86646266-86646285	None:intergenic	40.0%
	GTACCATTACTATCTCTGGA+AGG	+	chr4.4:86646184-86646203	MS.gene068297:CDS	40.0%
	GTTAGAGAGTTACCAGTGAT+CGG	+	chr4.4:86646225-86646244	MS.gene068297:CDS	40.0%
	GTTCCTTCCAGAGATAGTAA+TGG	-	chr4.4:86646190-86646209	None:intergenic	40.0%
	TGGTTGAATGATTGGAATGG+AGG	-	chr4.4:86645971-86645990	None:intergenic	40.0%
	TTCTACTGGCAAGACATAGT+GGG	+	chr4.4:86645931-86645950	MS.gene068297:CDS	40.0%
	TTGGGAAGTGGTTGAATGAT+TGG	-	chr4.4:86645979-86645998	None:intergenic	40.0%
	TTGTCCAGTGACAATTGTGA+GGG	-	chr4.4:86645839-86645858	None:intergenic	40.0%
!	AGCCCTACCTAACAATTTTG+AGG	-	chr4.4:86646076-86646095	None:intergenic	40.0%
!!!	TTAGTGCTTTTGGTTTGGTG+AGG	+	chr4.4:86646013-86646032	MS.gene068297:CDS	40.0%
	AAGGAACGTCGCAAACGATA+AGG	+	chr4.4:86646203-86646222	MS.gene068297:CDS	45.0%
	CTTGCCAGTAGAACCTAAAG+TGG	-	chr4.4:86645924-86645943	None:intergenic	45.0%
	CTTGTCCAGTGACAATTGTG+AGG	-	chr4.4:86645840-86645859	None:intergenic	45.0%
	GGGTGTAGAAAGAGAGTAGA+AGG	-	chr4.4:86645819-86645838	None:intergenic	45.0%
	GTTCTACTGGCAAGACATAG+TGG	+	chr4.4:86645930-86645949	MS.gene068297:CDS	45.0%
	TACACCCTCACAATTGTCAC+TGG	+	chr4.4:86645832-86645851	MS.gene068297:CDS	45.0%
!	AAGTAGCTGCTAGCCACTTT+AGG	+	chr4.4:86645908-86645927	MS.gene068297:CDS	45.0%
!	CTAGCCACTTTAGGTTCTAC+TGG	+	chr4.4:86645917-86645936	MS.gene068297:CDS	45.0%
!!	GGGAGTACCATTACTATCTC+TGG	+	chr4.4:86646180-86646199	MS.gene068297:CDS	45.0%
!!	TGAGGTCAAGTTTGGTGTCA+AGG	-	chr4.4:86646058-86646077	None:intergenic	45.0%
!!!	GGGGTTTTCCGATTTGCTAA+GGG	+	chr4.4:86646255-86646274	MS.gene068297:CDS	45.0%
	AGAGAGTTACCAGTGATCGG+TGG	+	chr4.4:86646228-86646247	MS.gene068297:CDS	50.0%
	GTATTCAACCACGGTATCCC+CGG	-	chr4.4:86646319-86646338	None:intergenic	50.0%
	GTCACTGGACAAGATGAGAC+CGG	+	chr4.4:86645847-86645866	MS.gene068297:CDS	50.0%
	TTACCAGTGATCGGTGGTAG+CGG	+	chr4.4:86646234-86646253	MS.gene068297:CDS	50.0%
	TTTCATAACCGGGGATACCG+TGG	+	chr4.4:86646308-86646327	MS.gene068297:CDS	50.0%
!	AAGCTCGGTTTGCGATGTAG+CGG	-	chr4.4:86646112-86646131	None:intergenic	50.0%
!	TACTGGCAAGACATAGTGGG+AGG	+	chr4.4:86645934-86645953	MS.gene068297:CDS	50.0%
!!!	CGGGGTTTTCCGATTTGCTA+AGG	+	chr4.4:86646254-86646273	MS.gene068297:CDS	50.0%
	TACCAGTGATCGGTGGTAGC+GGG	+	chr4.4:86646235-86646254	MS.gene068297:CDS	55.0%
!	ACCAGTGATCGGTGGTAGCG+GGG	+	chr4.4:86646236-86646255	MS.gene068297:CDS	60.0%
	ACCCCGCTACCACCGATCAC+TGG	-	chr4.4:86646240-86646259	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	86645787	86646365	86645787	ID=MS.gene068297
chr4.4	mRNA	86645787	86646365	86645787	ID=MS.gene068297.t1;Parent=MS.gene068297
chr4.4	exon	86645787	86646365	86645787	ID=MS.gene068297.t1.exon1;Parent=MS.gene068297.t1
chr4.4	CDS	86645787	86646365	86645787	ID=cds.MS.gene068297.t1;Parent=MS.gene068297.t1

Gene Sequence

>MS.gene068297

ATGAATACTTCCCAAAAACTTTTTTTCTTCCTTCTACTCTCTTTCTACACCCTCACAATTGTCACTGGACAAGATGAGACCGGTTTTGTAAGCTCAATAAATCCCAAGTTGCTTACAAAGAAAGTAGCTGCTAGCCACTTTAGGTTCTACTGGCAAGACATAGTGGGAGGAGCAAACGCAACCTCCATTCCAATCATTCAACCACTTCCCAAATTTAACAACTCTTTTAGTGCTTTTGGTTTGGTGAGGATAATCGACAACCCTTTAACCTTGACACCAAACTTGACCTCAAAATTGTTAGGTAGGGCTCAAGGGTTTTATGCCGCTACATCGCAAACCGAGCTTGATTTTCTTATGGTTATGAACTTTGCCTTGTTTGAAGGAAAGTATAATGGGAGTACCATTACTATCTCTGGAAGGAACGTCGCAAACGATAAGGTTAGAGAGTTACCAGTGATCGGTGGTAGCGGGGTTTTCCGATTTGCTAAGGGATATGCTGAAGCTAGAACAATCTCTTTTGATTTCATAACCGGGGATACCGTGGTTGAATACAATGTTTATGTTTCTCATCATAAATGA

Protein sequence

>MS.gene068297.t1

MNTSQKLFFFLLLSFYTLTIVTGQDETGFVSSINPKLLTKKVAASHFRFYWQDIVGGANATSIPIIQPLPKFNNSFSAFGLVRIIDNPLTLTPNLTSKLLGRAQGFYAATSQTELDFLMVMNFALFEGKYNGSTITISGRNVANDKVRELPVIGGSGVFRFAKGYAEARTISFDFITGDTVVEYNVYVSHHK