AlfalfaGEDB | MS.gene074462

M. sativa cultivar XinJiangDaYe / MS.gene074462

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074462.t1	XP_003627372.1	86.2	349	42	3	1	348	1	344	2.70E-143	518.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074462.t1	Q9LJG8	41.5	405	113	13	1	308	1	378	6.0e-43	176.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074462.t1	G7L8C7	86.2	349	42	3	1	348	1	344	1.9e-143	518.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene074462.t1	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene074462	0.801949	7.07E-49	-1.69E-46
MS.gene049159	MS.gene074462	0.810388	1.18E-50	-1.69E-46
MS.gene049368	MS.gene074462	0.805866	1.09E-49	-1.69E-46
MS.gene049983	MS.gene074462	0.805225	1.48E-49	-1.69E-46
MS.gene050084	MS.gene074462	0.820973	5.17E-53	-1.69E-46
MS.gene050085	MS.gene074462	0.836246	1.05E-56	-1.69E-46
MS.gene050317	MS.gene074462	0.824047	9.97E-54	-1.69E-46
MS.gene050361	MS.gene074462	0.811029	8.59E-51	-1.69E-46
MS.gene050384	MS.gene074462	0.802747	4.85E-49	-1.69E-46
MS.gene050386	MS.gene074462	0.823863	1.10E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074462.t1	MTR_8g022290	94.556	349	13	3	1	348	1	344	0.0	582
MS.gene074462.t1	MTR_2g016140	59.804	102	37	2	66	166	19	117	3.94e-36	134
MS.gene074462.t1	MTR_2g016140	50.649	77	35	1	267	343	236	309	5.42e-18	84.3
MS.gene074462.t1	MTR_2g016140	59.804	102	37	2	66	166	19	117	5.66e-36	134
MS.gene074462.t1	MTR_2g016140	48.837	86	40	2	267	352	236	317	8.87e-18	84.0
MS.gene074462.t1	MTR_4g079960	46.541	159	51	5	21	174	5	134	5.39e-35	130
MS.gene074462.t1	MTR_4g079960	54.688	64	29	0	280	343	234	297	5.46e-17	81.3
MS.gene074462.t1	MTR_4g079960	46.541	159	51	5	21	174	5	134	7.35e-35	130
MS.gene074462.t1	MTR_4g079960	54.688	64	29	0	280	343	234	297	7.62e-17	80.9
MS.gene074462.t1	MTR_4g079960	46.541	159	51	5	21	174	5	134	8.77e-35	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074462.t1	AT3G14180	46.602	412	122	13	34	357	24	425	5.16e-96	293
MS.gene074462.t1	AT1G54060	39.012	405	181	12	1	361	1	383	1.45e-57	192
MS.gene074462.t1	AT3G11100	41.343	283	105	7	64	342	16	241	3.78e-47	161
MS.gene074462.t1	AT5G05550	38.832	291	115	8	65	353	19	248	6.20e-46	158
MS.gene074462.t1	AT5G05550	39.161	286	111	8	65	348	19	243	1.01e-44	154
MS.gene074462.t1	AT5G05550	39.493	276	105	7	65	338	19	234	1.37e-44	154
MS.gene074462.t1	AT3G58630	47.333	150	63	5	66	203	22	167	1.33e-32	124
MS.gene074462.t1	AT3G58630	47.333	150	63	5	66	203	22	167	1.38e-32	124
MS.gene074462.t1	AT3G10030	37.168	113	65	2	70	182	161	267	1.69e-18	87.4
MS.gene074462.t1	AT3G10030	37.168	113	65	2	70	182	161	267	2.00e-18	87.0

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Find 105 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCGGAGAAAGCTAAGAT+CGG	0.619321	8.2:+73412463	MS.gene074462:CDS
AAATTCGAAGGGGAGAGGTT+GGG	0.373866	8.2:+73412870	MS.gene074462:CDS
AAGCAACGGTGGAGGGAGGG+AGG	0.596019	8.2:+73412243	MS.gene074462:CDS
AAGCGACAGTTTACCACCAC+CGG	0.595248	8.2:+73412813	MS.gene074462:CDS
AATACGACACGTGGAAGCAA+CGG	0.563694	8.2:+73412229	MS.gene074462:CDS
AATGCATAGCTGTTCGCCGC+CGG	0.453869	8.2:-73412190	None:intergenic
AATTCAAATTCGAAGGGGAG+AGG	0.518146	8.2:+73412865	MS.gene074462:CDS
AATTCGAAGGGGAGAGGTTG+GGG	0.424508	8.2:+73412871	MS.gene074462:CDS
AATTTGAATTCAAATTCGAA+GGG	0.573920	8.2:+73412859	MS.gene074462:CDS
ACACAGGCAATTGTGAAATT+TGG	0.247863	8.2:+73412913	MS.gene074462:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGATATTCAGTGTAAGAAT+CGG	+	chr8.2:73412416-73412435	MS.gene074462:CDS	30.0%
AAATCGGAGAAAGCTAAGAT+CGG	+	chr8.2:73412463-73412482	MS.gene074462:CDS	35.0%
ACACAGGCAATTGTGAAATT+TGG	+	chr8.2:73412913-73412932	MS.gene074462:CDS	35.0%
ATACCAACAGGAATTCCTAA+CGG	-	chr8.2:73412634-73412653	None:intergenic	35.0%
CACTGTCACTGTTATTGTTA+TGG	-	chr8.2:73413170-73413189	None:intergenic	35.0%
GAGATTTCACAGTTGAAACT+TGG	+	chr8.2:73413060-73413079	MS.gene074462:CDS	35.0%
GATCGCTTAGATCAGTTAAT+TGG	+	chr8.2:73412535-73412554	MS.gene074462:CDS	35.0%
GTGTAAGAATCGGATTGATA+CGG	+	chr8.2:73412426-73412445	MS.gene074462:CDS	35.0%
AATTCAAATTCGAAGGGGAG+AGG	+	chr8.2:73412865-73412884	MS.gene074462:CDS	40.0%
CTGTGACAAATGACTGTTTC+CGG	-	chr8.2:73412602-73412621	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	73412064	73413194	73412064	ID=MS.gene074462
chr8.2	mRNA	73412064	73413194	73412064	ID=MS.gene074462.t1;Parent=MS.gene074462
chr8.2	exon	73412064	73413194	73412064	ID=MS.gene074462.t1.exon1;Parent=MS.gene074462.t1
chr8.2	CDS	73412064	73413194	73412064	ID=cds.MS.gene074462.t1;Parent=MS.gene074462.t1

Gene Sequence

>MS.gene074462

ATGGATGACGACGACGAGATCCACTCGGCTAACTCGATCCACTCGAATCCATCTCCGGCGAGTGGTTCTCCATCTCCGGTGTCGTCTCCGGGAGCGATTGGTCGGATTTCGGTGACGGTTGCGGCACCGGCGGCGAACAGCTATGCATTAGCGCTTCCGATTCAGAATACGACACGTGGAAGCAACGGTGGAGGGAGGGAGGATTGTTGGAGCGAAGCAGCGACGGCGGTGTTGATTGAAGCGTGGGGAGAGAGGTATTTGGAATTGAGCAGAGGGAATTTGAAGCAGAAGCATTGGAAAGAGGTTGCGGAGATTGTTAATGGAAGAGGTGATTATTTGAAAGCACCGAAAACTGATATTCAGTGTAAGAATCGGATTGATACGGTTAAGAAGAAGTATAAATCGGAGAAAGCTAAGATCGGTGCTGGAAGTAGCTCCGGCGGTGTTACAACCAGCACTTGGCGGTTTTATGATCGCTTAGATCAGTTAATTGGTCCAGCTGCCAAGGTTAGTGGTGTTTCCGGTACCAGTCATACCGGAAACAGTCATTTGTCACAGCAGAAAGTTCCGTTAGGAATTCCTGTTGGTATTCGCGCCTCTGGAGCTAGAGGCGCGAATCAGGTTAATTCGCAGAAGAAGGATATTCATCAAGCTCAGTTGAATTATCAGAAGATTCAGCTCAGACCACGAGTTTCTGAGCTGAATTCTTCTGATAATTCATCTGAGAAAGAAGCGTTGTCACCGGTGTCAAGCGACAGTTTACCACCACCGGAGAGGAAGAGAGCGAAAGTGATGAATTTGAATTCAAATTCGAAGGGGAGAGGTTGGGGAAGTGCGGTGAGGGAATTGACACAGGCAATTGTGAAATTTGGTGAAGCTTATGAACAAGCGGAGACATCGAAGTTGCAGCAGGTGGTGGAGATGGAGAAGCAGAGGATGAAGTTTGCTAAGGATTTGGAGTTGCAGAGAATGCAGTTTTTCATGAAAACTCAGGTAGAGATTTCACAGTTGAAACTTGGAAGAAAAAGTGTTAATGTTAATGGTAACACAAGTAATCATCAAAACAACAACAATGTAACCAGCAACAACAACAACAATAGCAACCATAACAATAACAGTGACAGTGAGTGA

Protein sequence

>MS.gene074462.t1

MDDDDEIHSANSIHSNPSPASGSPSPVSSPGAIGRISVTVAAPAANSYALALPIQNTTRGSNGGGREDCWSEAATAVLIEAWGERYLELSRGNLKQKHWKEVAEIVNGRGDYLKAPKTDIQCKNRIDTVKKKYKSEKAKIGAGSSSGGVTTSTWRFYDRLDQLIGPAAKVSGVSGTSHTGNSHLSQQKVPLGIPVGIRASGARGANQVNSQKKDIHQAQLNYQKIQLRPRVSELNSSDNSSEKEALSPVSSDSLPPPERKRAKVMNLNSNSKGRGWGSAVRELTQAIVKFGEAYEQAETSKLQQVVEMEKQRMKFAKDLELQRMQFFMKTQVEISQLKLGRKSVNVNGNTSNHQNNNNVTSNNNNNSNHNNNSDSE