Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08721.t1 | XP_003606407.1 | 93.6 | 218 | 14 | 0 | 1 | 218 | 1 | 218 | 6.50E-118 | 433.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08721.t1 | Q9SR36 | 45.6 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 3.6e-48 | 193.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08721.t1 | G7JPE9 | 93.6 | 218 | 14 | 0 | 1 | 218 | 1 | 218 | 4.7e-118 | 433.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene08721 | -0.801553 | 8.53E-49 | -1.69E-46 |
| MS.gene049281 | MS.gene08721 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene08721 | 0.815159 | 1.07E-51 | -1.69E-46 |
| MS.gene050082 | MS.gene08721 | 0.834272 | 3.30E-56 | -1.69E-46 |
| MS.gene050323 | MS.gene08721 | -0.802046 | 6.75E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene08721 | 0.828589 | 8.27E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene08721 | 0.806576 | 7.69E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene08721 | -0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene08721 | -0.808009 | 3.82E-50 | -1.69E-46 |
| MS.gene05079 | MS.gene08721 | 0.810208 | 1.29E-50 | -1.69E-46 |
| MS.gene050809 | MS.gene08721 | 0.828542 | 8.48E-55 | -1.69E-46 |
| MS.gene050832 | MS.gene08721 | 0.800702 | 1.27E-48 | -1.69E-46 |
| MS.gene050833 | MS.gene08721 | 0.809446 | 1.89E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene08721 | 0.810694 | 1.01E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene08721 | 0.816967 | 4.21E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene08721 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene08721 | 0.851493 | 8.56E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene08721 | 0.827699 | 1.35E-54 | -1.69E-46 |
| MS.gene051366 | MS.gene08721 | 0.830386 | 3.02E-55 | -1.69E-46 |
| MS.gene051422 | MS.gene08721 | 0.857181 | 1.94E-62 | -1.69E-46 |
| MS.gene051651 | MS.gene08721 | 0.832818 | 7.61E-56 | -1.69E-46 |
| MS.gene051749 | MS.gene08721 | 0.805512 | 1.29E-49 | -1.69E-46 |
| MS.gene052129 | MS.gene08721 | 0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene08721 | 0.808854 | 2.52E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene08721 | 0.837038 | 6.58E-57 | -1.69E-46 |
| MS.gene054056 | MS.gene08721 | 0.80868 | 2.75E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene08721 | 0.825286 | 5.09E-54 | -1.69E-46 |
| MS.gene055154 | MS.gene08721 | 0.809927 | 1.49E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene08721 | -0.813643 | 2.31E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene08721 | 0.853168 | 2.86E-61 | -1.69E-46 |
| MS.gene056224 | MS.gene08721 | 0.810217 | 1.29E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene08721 | -0.819752 | 9.85E-53 | -1.69E-46 |
| MS.gene056590 | MS.gene08721 | 0.816253 | 6.08E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene08721 | 0.815754 | 7.86E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene08721 | 0.812103 | 5.02E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene08721 | 0.879203 | 1.51E-69 | -1.69E-46 |
| MS.gene056857 | MS.gene08721 | 0.847835 | 8.99E-60 | -1.69E-46 |
| MS.gene057548 | MS.gene08721 | 0.821515 | 3.88E-53 | -1.69E-46 |
| MS.gene057549 | MS.gene08721 | 0.855868 | 4.73E-62 | -1.69E-46 |
| MS.gene058067 | MS.gene08721 | 0.83494 | 2.24E-56 | -1.69E-46 |
| MS.gene058068 | MS.gene08721 | 0.8296 | 4.70E-55 | -1.69E-46 |
| MS.gene058093 | MS.gene08721 | 0.815755 | 7.86E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene08721 | 0.850007 | 2.24E-60 | -1.69E-46 |
| MS.gene058117 | MS.gene08721 | 0.800847 | 1.19E-48 | -1.69E-46 |
| MS.gene058268 | MS.gene08721 | 0.871391 | 7.08E-67 | -1.69E-46 |
| MS.gene058269 | MS.gene08721 | 0.845674 | 3.50E-59 | -1.69E-46 |
| MS.gene058334 | MS.gene08721 | 0.816202 | 6.25E-52 | -1.69E-46 |
| MS.gene058382 | MS.gene08721 | 0.830896 | 2.27E-55 | -1.69E-46 |
| MS.gene058603 | MS.gene08721 | 0.817186 | 3.76E-52 | -1.69E-46 |
| MS.gene05926 | MS.gene08721 | 0.806457 | 8.15E-50 | -1.69E-46 |
| MS.gene059315 | MS.gene08721 | 0.813911 | 2.01E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene08721 | -0.804655 | 1.95E-49 | -1.69E-46 |
| MS.gene059685 | MS.gene08721 | 0.803979 | 2.69E-49 | -1.69E-46 |
| MS.gene059699 | MS.gene08721 | 0.831833 | 1.33E-55 | -1.69E-46 |
| MS.gene059703 | MS.gene08721 | 0.832622 | 8.51E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene08721 | -0.816304 | 5.93E-52 | -1.69E-46 |
| MS.gene059953 | MS.gene08721 | 0.804935 | 1.70E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene08721 | 0.810079 | 1.38E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene08721 | -0.816596 | 5.10E-52 | -1.69E-46 |
| MS.gene060823 | MS.gene08721 | 0.826187 | 3.11E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene08721 | -0.801654 | 8.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08721.t1 | MTR_4g059730 | 93.578 | 218 | 14 | 0 | 1 | 218 | 1 | 218 | 1.35e-152 | 422 |
| MS.gene08721.t1 | MTR_1g090060 | 68.372 | 215 | 68 | 0 | 4 | 218 | 3 | 217 | 4.90e-111 | 317 |
| MS.gene08721.t1 | MTR_1g090100 | 66.822 | 214 | 71 | 0 | 5 | 218 | 3 | 216 | 1.45e-105 | 303 |
| MS.gene08721.t1 | MTR_1g090070 | 63.426 | 216 | 78 | 1 | 4 | 218 | 9 | 224 | 2.08e-102 | 296 |
| MS.gene08721.t1 | MTR_1g090090 | 65.581 | 215 | 74 | 0 | 4 | 218 | 3 | 217 | 3.14e-102 | 295 |
| MS.gene08721.t1 | MTR_1g115195 | 49.302 | 215 | 105 | 3 | 1 | 213 | 1 | 213 | 6.71e-67 | 205 |
| MS.gene08721.t1 | MTR_1g090150 | 48.341 | 211 | 108 | 1 | 3 | 213 | 2 | 211 | 1.48e-66 | 204 |
| MS.gene08721.t1 | MTR_4g019790 | 45.283 | 212 | 112 | 2 | 4 | 214 | 7 | 215 | 8.29e-62 | 192 |
| MS.gene08721.t1 | MTR_4g019780 | 46.083 | 217 | 113 | 3 | 3 | 218 | 7 | 220 | 7.06e-61 | 190 |
| MS.gene08721.t1 | MTR_7g065660 | 46.479 | 213 | 110 | 2 | 3 | 214 | 6 | 215 | 3.71e-59 | 186 |
| MS.gene08721.t1 | MTR_7g065740 | 43.925 | 214 | 115 | 2 | 3 | 214 | 6 | 216 | 1.61e-58 | 184 |
| MS.gene08721.t1 | MTR_7g065230 | 45.370 | 216 | 109 | 3 | 3 | 217 | 6 | 213 | 3.23e-58 | 183 |
| MS.gene08721.t1 | MTR_7g065720 | 44.393 | 214 | 114 | 2 | 3 | 214 | 6 | 216 | 3.81e-57 | 181 |
| MS.gene08721.t1 | MTR_5g040430 | 44.091 | 220 | 117 | 4 | 2 | 216 | 5 | 223 | 1.12e-56 | 179 |
| MS.gene08721.t1 | MTR_3g099757 | 44.601 | 213 | 114 | 3 | 3 | 214 | 4 | 213 | 3.87e-56 | 178 |
| MS.gene08721.t1 | MTR_7g065590 | 42.523 | 214 | 117 | 3 | 3 | 214 | 6 | 215 | 7.57e-56 | 177 |
| MS.gene08721.t1 | MTR_8g087425 | 44.651 | 215 | 113 | 2 | 2 | 214 | 3 | 213 | 7.49e-55 | 175 |
| MS.gene08721.t1 | MTR_7g065750 | 42.056 | 214 | 119 | 2 | 3 | 214 | 6 | 216 | 2.14e-54 | 174 |
| MS.gene08721.t1 | MTR_8g087410 | 46.396 | 222 | 110 | 5 | 1 | 218 | 2 | 218 | 3.60e-53 | 171 |
| MS.gene08721.t1 | MTR_2g070120 | 42.254 | 213 | 118 | 3 | 1 | 212 | 1 | 209 | 2.94e-52 | 168 |
| MS.gene08721.t1 | MTR_7g065270 | 42.056 | 214 | 118 | 4 | 3 | 214 | 6 | 215 | 8.59e-52 | 167 |
| MS.gene08721.t1 | MTR_2g070150 | 45.455 | 209 | 107 | 4 | 1 | 207 | 1 | 204 | 1.16e-51 | 166 |
| MS.gene08721.t1 | MTR_7g065600 | 39.437 | 213 | 125 | 2 | 3 | 214 | 6 | 215 | 2.74e-51 | 166 |
| MS.gene08721.t1 | MTR_8g061950 | 43.578 | 218 | 115 | 6 | 5 | 215 | 6 | 222 | 1.66e-50 | 164 |
| MS.gene08721.t1 | MTR_7g065290 | 40.376 | 213 | 123 | 2 | 3 | 214 | 6 | 215 | 3.25e-50 | 163 |
| MS.gene08721.t1 | MTR_6g080440 | 43.434 | 198 | 106 | 3 | 18 | 213 | 17 | 210 | 4.81e-50 | 162 |
| MS.gene08721.t1 | MTR_7g065630 | 40.376 | 213 | 119 | 3 | 3 | 214 | 24 | 229 | 5.01e-50 | 163 |
| MS.gene08721.t1 | MTR_7g065640 | 41.784 | 213 | 102 | 3 | 3 | 214 | 6 | 197 | 5.62e-50 | 162 |
| MS.gene08721.t1 | MTR_7g065710 | 41.395 | 215 | 116 | 4 | 1 | 213 | 1 | 207 | 6.45e-50 | 162 |
| MS.gene08721.t1 | MTR_7g065265 | 40.845 | 213 | 122 | 2 | 3 | 214 | 6 | 215 | 3.23e-49 | 160 |
| MS.gene08721.t1 | MTR_2g070200 | 42.793 | 222 | 118 | 6 | 1 | 218 | 1 | 217 | 3.52e-49 | 160 |
| MS.gene08721.t1 | MTR_7g065700 | 42.056 | 214 | 116 | 3 | 1 | 213 | 4 | 210 | 7.88e-49 | 159 |
| MS.gene08721.t1 | MTR_2g070130 | 40.670 | 209 | 119 | 3 | 1 | 208 | 1 | 205 | 6.32e-48 | 157 |
| MS.gene08721.t1 | MTR_2g070180 | 42.381 | 210 | 114 | 4 | 1 | 208 | 1 | 205 | 8.62e-48 | 156 |
| MS.gene08721.t1 | MTR_7g065260 | 43.256 | 215 | 115 | 5 | 3 | 214 | 13 | 223 | 2.89e-47 | 155 |
| MS.gene08721.t1 | MTR_2g070210 | 41.204 | 216 | 118 | 5 | 1 | 212 | 1 | 211 | 4.13e-47 | 155 |
| MS.gene08721.t1 | MTR_2g070140 | 43.137 | 204 | 109 | 4 | 17 | 218 | 1 | 199 | 4.66e-46 | 151 |
| MS.gene08721.t1 | MTR_7g065680 | 39.906 | 213 | 120 | 3 | 3 | 214 | 6 | 211 | 1.28e-44 | 148 |
| MS.gene08721.t1 | MTR_8g056940 | 39.906 | 213 | 123 | 3 | 1 | 212 | 1 | 209 | 2.03e-44 | 148 |
| MS.gene08721.t1 | MTR_3g467420 | 40.476 | 210 | 120 | 3 | 10 | 218 | 15 | 220 | 1.31e-43 | 146 |
| MS.gene08721.t1 | MTR_5g037380 | 38.222 | 225 | 130 | 4 | 3 | 218 | 4 | 228 | 3.72e-43 | 145 |
| MS.gene08721.t1 | MTR_4g124130 | 40.000 | 220 | 126 | 4 | 1 | 218 | 1 | 216 | 1.52e-42 | 143 |
| MS.gene08721.t1 | MTR_3g467430 | 39.631 | 217 | 126 | 3 | 3 | 218 | 8 | 220 | 1.74e-42 | 143 |
| MS.gene08721.t1 | MTR_2g070060 | 38.426 | 216 | 128 | 3 | 1 | 215 | 1 | 212 | 2.98e-42 | 142 |
| MS.gene08721.t1 | MTR_2g070070 | 40.376 | 213 | 122 | 3 | 1 | 212 | 1 | 209 | 7.23e-42 | 141 |
| MS.gene08721.t1 | MTR_2g070110 | 41.232 | 211 | 114 | 5 | 1 | 208 | 1 | 204 | 2.78e-40 | 137 |
| MS.gene08721.t1 | MTR_5g076900 | 36.792 | 212 | 129 | 3 | 2 | 212 | 4 | 211 | 3.80e-38 | 132 |
| MS.gene08721.t1 | MTR_0186s0030 | 46.324 | 136 | 72 | 1 | 1 | 135 | 1 | 136 | 1.62e-35 | 122 |
| MS.gene08721.t1 | MTR_2g072120 | 60.714 | 56 | 21 | 1 | 48 | 102 | 2 | 57 | 2.92e-19 | 79.0 |
| MS.gene08721.t1 | MTR_5g037390 | 42.857 | 91 | 42 | 2 | 28 | 115 | 11 | 94 | 3.53e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08721.t1 | AT3G09270 | 45.581 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 6.93e-59 | 185 |
| MS.gene08721.t1 | AT2G29450 | 43.578 | 218 | 119 | 3 | 3 | 218 | 5 | 220 | 3.79e-56 | 178 |
| MS.gene08721.t1 | AT2G29460 | 43.318 | 217 | 120 | 2 | 3 | 218 | 6 | 220 | 6.52e-56 | 177 |
| MS.gene08721.t1 | AT2G29490 | 43.981 | 216 | 118 | 2 | 3 | 217 | 6 | 219 | 1.68e-55 | 176 |
| MS.gene08721.t1 | AT1G10370 | 43.694 | 222 | 120 | 3 | 2 | 218 | 3 | 224 | 2.63e-55 | 176 |
| MS.gene08721.t1 | AT2G29470 | 42.661 | 218 | 121 | 3 | 3 | 218 | 6 | 221 | 1.12e-54 | 174 |
| MS.gene08721.t1 | AT2G29420 | 40.092 | 217 | 128 | 1 | 2 | 218 | 7 | 221 | 3.02e-54 | 173 |
| MS.gene08721.t1 | AT2G29480 | 43.779 | 217 | 119 | 2 | 3 | 218 | 6 | 220 | 7.91e-54 | 172 |
| MS.gene08721.t1 | AT1G17180 | 41.096 | 219 | 124 | 3 | 1 | 218 | 1 | 215 | 4.34e-49 | 160 |
| MS.gene08721.t1 | AT2G29440 | 40.092 | 217 | 127 | 2 | 3 | 218 | 5 | 219 | 1.61e-48 | 159 |
| MS.gene08721.t1 | AT1G78340 | 41.981 | 212 | 118 | 3 | 1 | 211 | 1 | 208 | 3.18e-48 | 157 |
| MS.gene08721.t1 | AT1G10360 | 41.176 | 221 | 125 | 3 | 3 | 218 | 4 | 224 | 4.26e-48 | 157 |
| MS.gene08721.t1 | AT1G27130 | 40.553 | 217 | 123 | 4 | 3 | 213 | 5 | 221 | 7.57e-48 | 157 |
| MS.gene08721.t1 | AT1G74590 | 39.269 | 219 | 130 | 2 | 3 | 218 | 6 | 224 | 4.06e-47 | 155 |
| MS.gene08721.t1 | AT1G78320 | 40.566 | 212 | 117 | 6 | 1 | 209 | 1 | 206 | 6.57e-46 | 152 |
| MS.gene08721.t1 | AT1G78370 | 39.623 | 212 | 123 | 3 | 1 | 211 | 1 | 208 | 1.26e-45 | 151 |
| MS.gene08721.t1 | AT5G62480 | 36.726 | 226 | 128 | 5 | 3 | 217 | 7 | 228 | 1.61e-45 | 151 |
| MS.gene08721.t1 | AT1G69930 | 37.674 | 215 | 130 | 3 | 3 | 213 | 11 | 225 | 6.26e-45 | 150 |
| MS.gene08721.t1 | AT1G59670 | 39.631 | 217 | 125 | 4 | 3 | 213 | 5 | 221 | 9.59e-45 | 149 |
| MS.gene08721.t1 | AT1G27140 | 39.910 | 223 | 127 | 4 | 3 | 218 | 5 | 227 | 1.17e-44 | 149 |
| MS.gene08721.t1 | AT1G78380 | 39.726 | 219 | 127 | 3 | 1 | 218 | 1 | 215 | 1.03e-43 | 146 |
| MS.gene08721.t1 | AT1G17170 | 39.906 | 213 | 122 | 4 | 1 | 212 | 1 | 208 | 3.94e-43 | 144 |
| MS.gene08721.t1 | AT1G59700 | 37.327 | 217 | 130 | 4 | 3 | 213 | 5 | 221 | 4.06e-42 | 142 |
| MS.gene08721.t1 | AT1G17190 | 35.945 | 217 | 134 | 3 | 3 | 218 | 4 | 216 | 2.66e-41 | 140 |
| MS.gene08721.t1 | AT1G78360 | 37.850 | 214 | 127 | 4 | 1 | 212 | 1 | 210 | 6.56e-39 | 134 |
| MS.gene08721.t1 | AT1G69920 | 35.814 | 215 | 132 | 4 | 5 | 213 | 35 | 249 | 2.09e-38 | 134 |
| MS.gene08721.t1 | AT1G53680 | 38.119 | 202 | 118 | 4 | 10 | 208 | 13 | 210 | 6.29e-38 | 131 |
| MS.gene08721.t1 | AT3G43800 | 38.182 | 220 | 127 | 5 | 3 | 217 | 4 | 219 | 2.42e-37 | 130 |
| MS.gene08721.t1 | AT5G62480 | 30.222 | 225 | 117 | 5 | 3 | 217 | 7 | 201 | 2.35e-29 | 109 |
| MS.gene08721.t1 | AT5G62480 | 30.222 | 225 | 118 | 5 | 3 | 217 | 7 | 202 | 2.99e-29 | 108 |
| MS.gene08721.t1 | AT1G78320 | 32.701 | 211 | 108 | 6 | 1 | 209 | 1 | 179 | 2.53e-27 | 103 |
| MS.gene08721.t1 | AT2G02390 | 26.852 | 216 | 137 | 6 | 3 | 206 | 7 | 213 | 3.59e-11 | 60.8 |
Find 38 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCCCAAGCTCGGTTC+TGG | 0.280479 | 4.2:-43883249 | MS.gene08721:CDS |
| AAGTGAAGCTGCATGGATTT+TGG | 0.284087 | 4.2:-43883507 | MS.gene08721:CDS |
| AGGAGACACCGTTAACATTC+TGG | 0.299490 | 4.2:-43882785 | MS.gene08721:CDS |
| AATACTACCCTCAAAGTAAA+AGG | 0.302524 | 4.2:+43883478 | None:intergenic |
| CCGAGCTTGGGCTCTCTCAT+AGG | 0.329353 | 4.2:+43883255 | None:intergenic |
| AGAATACCATAGCGAAAATC+TGG | 0.331408 | 4.2:-43882864 | MS.gene08721:CDS |
| GTATTGACCCTTAAGCTAAA+AGG | 0.363440 | 4.2:-43883460 | MS.gene08721:CDS |
| TTGAATATATTGATGAGATA+TGG | 0.397859 | 4.2:-43883306 | MS.gene08721:CDS |
| AACATTCTGGACATAGCTCT+TGG | 0.422694 | 4.2:-43882772 | MS.gene08721:CDS |
| AAAACTCCGGTGTTGGTTCA+TGG | 0.434187 | 4.2:-43883361 | MS.gene08721:CDS |
| AGCTATGTCCAGAATGTTAA+CGG | 0.440075 | 4.2:+43882777 | None:intergenic |
| GCTATCAGTTTCTCATGATC+AGG | 0.440168 | 4.2:+43882619 | None:intergenic |
| GCTCTCTCATAGGGATCAAC+AGG | 0.444906 | 4.2:+43883265 | None:intergenic |
| AATTCCCTCACTTGCATGCA+TGG | 0.474769 | 4.2:-43882684 | MS.gene08721:CDS |
| TTCAAGAATGTACCTGTCAT+AGG | 0.475029 | 4.2:-43882652 | MS.gene08721:CDS |
| TCTTCCTCTATGTTCTCATA+TGG | 0.483131 | 4.2:+43883433 | None:intergenic |
| GAGAGCCCAAGCTCGGTTCT+GGG | 0.483641 | 4.2:-43883248 | MS.gene08721:CDS |
| CGAGCTTGGGCTCTCTCATA+GGG | 0.521012 | 4.2:+43883256 | None:intergenic |
| TCAAACCATGCATGCAAGTG+AGG | 0.535813 | 4.2:+43882679 | None:intergenic |
| GAGGGAATTTCTCAGCTGCT+AGG | 0.567585 | 4.2:+43882698 | None:intergenic |
| CAAACCATGCATGCAAGTGA+GGG | 0.568010 | 4.2:+43882680 | None:intergenic |
| TAGGGATCAACAGGAAGCAA+AGG | 0.573576 | 4.2:+43883274 | None:intergenic |
| GCACAAGAAAACTCCGGTGT+TGG | 0.576241 | 4.2:-43883368 | MS.gene08721:CDS |
| AGGGATCAACAGGAAGCAAA+GGG | 0.577972 | 4.2:+43883275 | None:intergenic |
| CTAACGAGTGCACCAACTGC+AGG | 0.579048 | 4.2:+43882910 | None:intergenic |
| GAATACCATAGCGAAAATCT+GGG | 0.582158 | 4.2:-43882863 | MS.gene08721:CDS |
| GGATTGTATTGAAGAAGTTG+AGG | 0.589460 | 4.2:+43883394 | None:intergenic |
| GCAGATCAAGTGAAGCTGCA+TGG | 0.593947 | 4.2:-43883514 | MS.gene08721:CDS |
| GGTGCACTCGTTAGTAGCGG+TGG | 0.611355 | 4.2:-43882901 | MS.gene08721:CDS |
| CCTATGAGAGAGCCCAAGCT+CGG | 0.617875 | 4.2:-43883255 | MS.gene08721:CDS |
| TTTCCACCATGAACCAACAC+CGG | 0.639081 | 4.2:+43883355 | None:intergenic |
| ATGATCATAGACTCACATAG+AGG | 0.640551 | 4.2:+43883331 | None:intergenic |
| GTAGCGGTGGCGATGAAGAG+CGG | 0.649339 | 4.2:-43882888 | MS.gene08721:CDS |
| TCCAGTGCACAAGAAAACTC+CGG | 0.653047 | 4.2:-43883374 | MS.gene08721:CDS |
| ACTCCGGTGTTGGTTCATGG+TGG | 0.663999 | 4.2:-43883358 | MS.gene08721:CDS |
| GTTGGTGCACTCGTTAGTAG+CGG | 0.664719 | 4.2:-43882904 | MS.gene08721:CDS |
| ACAGGAAGCAAAGGGTACTG+TGG | 0.666663 | 4.2:+43883283 | None:intergenic |
| TATACCATATGAGAACATAG+AGG | 0.675165 | 4.2:-43883437 | MS.gene08721:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAATCAACATATTATTT+AGG | - | chr4.2:43883013-43883032 | MS.gene08721:intron | 10.0% |
| !! | TTATCATATTGAATAAATTT+AGG | + | chr4.2:43883134-43883153 | None:intergenic | 10.0% |
| !! | AAAAATAATATTGTTGGATA+TGG | + | chr4.2:43883089-43883108 | None:intergenic | 15.0% |
| !!! | AAATTACTATTACTTTTTCA+TGG | + | chr4.2:43882950-43882969 | None:intergenic | 15.0% |
| !! | TTGAATATATTGATGAGATA+TGG | - | chr4.2:43882802-43882821 | MS.gene08721:CDS | 20.0% |
| AATACTACCCTCAAAGTAAA+AGG | + | chr4.2:43882633-43882652 | None:intergenic | 30.0% | |
| GCTAAGAAAATTGCAAATGA+AGG | + | chr4.2:43883052-43883071 | None:intergenic | 30.0% | |
| TATACCATATGAGAACATAG+AGG | - | chr4.2:43882671-43882690 | MS.gene08721:CDS | 30.0% | |
| ! | GAAACTGATAGCTTTTATGA+AGG | - | chr4.2:43883500-43883519 | MS.gene08721:CDS | 30.0% |
| ! | TTTTCATGGTATGCAAATGA+AGG | + | chr4.2:43882936-43882955 | None:intergenic | 30.0% |
| ! | TTTTTATAGATTCCTGCAGT+TGG | - | chr4.2:43883186-43883205 | MS.gene08721:intron | 30.0% |
| !!! | ATGGTATACCTTTTAGCTTA+AGG | + | chr4.2:43882659-43882678 | None:intergenic | 30.0% |
| !!! | TGGTATACCTTTTAGCTTAA+GGG | + | chr4.2:43882658-43882677 | None:intergenic | 30.0% |
| AGAATACCATAGCGAAAATC+TGG | - | chr4.2:43883244-43883263 | MS.gene08721:CDS | 35.0% | |
| AGCTATGTCCAGAATGTTAA+CGG | + | chr4.2:43883334-43883353 | None:intergenic | 35.0% | |
| ATGATCATAGACTCACATAG+AGG | + | chr4.2:43882780-43882799 | None:intergenic | 35.0% | |
| GAATACCATAGCGAAAATCT+GGG | - | chr4.2:43883245-43883264 | MS.gene08721:CDS | 35.0% | |
| GGATATGGTATCAACTATCA+AGG | + | chr4.2:43883074-43883093 | None:intergenic | 35.0% | |
| GGATTGTATTGAAGAAGTTG+AGG | + | chr4.2:43882717-43882736 | None:intergenic | 35.0% | |
| TCTTCCTCTATGTTCTCATA+TGG | + | chr4.2:43882678-43882697 | None:intergenic | 35.0% | |
| TTCAAGAATGTACCTGTCAT+AGG | - | chr4.2:43883456-43883475 | MS.gene08721:CDS | 35.0% | |
| ! | GACTACGAGAAGAAACTTTT+TGG | - | chr4.2:43883300-43883319 | MS.gene08721:CDS | 35.0% |
| ! | GTACAGTCCTTTTACTTTGA+GGG | - | chr4.2:43882623-43882642 | MS.gene08721:CDS | 35.0% |
| ! | GTATTGACCCTTAAGCTAAA+AGG | - | chr4.2:43882648-43882667 | MS.gene08721:CDS | 35.0% |
| ! | TACGAGAAGAAACTTTTTGG+AGG | - | chr4.2:43883303-43883322 | MS.gene08721:CDS | 35.0% |
| AACATTCTGGACATAGCTCT+TGG | - | chr4.2:43883336-43883355 | MS.gene08721:CDS | 40.0% | |
| GCTATCAGTTTCTCATGATC+AGG | + | chr4.2:43883492-43883511 | None:intergenic | 40.0% | |
| ! | AAGTGAAGCTGCATGGATTT+TGG | - | chr4.2:43882601-43882620 | MS.gene08721:CDS | 40.0% |
| ! | ATGTTCCCAGATTTTCGCTA+TGG | + | chr4.2:43883253-43883272 | None:intergenic | 40.0% |
| ! | GGAAGATTTTCACCTATGAC+AGG | + | chr4.2:43883471-43883490 | None:intergenic | 40.0% |
| ! | GGTACAGTCCTTTTACTTTG+AGG | - | chr4.2:43882622-43882641 | MS.gene08721:CDS | 40.0% |
| AATTCCCTCACTTGCATGCA+TGG | - | chr4.2:43883424-43883443 | MS.gene08721:CDS | 45.0% | |
| AGGAGACACCGTTAACATTC+TGG | - | chr4.2:43883323-43883342 | MS.gene08721:CDS | 45.0% | |
| AGGGATCAACAGGAAGCAAA+GGG | + | chr4.2:43882836-43882855 | None:intergenic | 45.0% | |
| CAAACCATGCATGCAAGTGA+GGG | + | chr4.2:43883431-43883450 | None:intergenic | 45.0% | |
| TAGGGATCAACAGGAAGCAA+AGG | + | chr4.2:43882837-43882856 | None:intergenic | 45.0% | |
| TCAAACCATGCATGCAAGTG+AGG | + | chr4.2:43883432-43883451 | None:intergenic | 45.0% | |
| TCCAGTGCACAAGAAAACTC+CGG | - | chr4.2:43882734-43882753 | MS.gene08721:CDS | 45.0% | |
| TTTCCACCATGAACCAACAC+CGG | + | chr4.2:43882756-43882775 | None:intergenic | 45.0% | |
| ! | ATTTTACCCAGAACCGAGCT+TGG | + | chr4.2:43882869-43882888 | None:intergenic | 45.0% |
| ! | TTTTACCCAGAACCGAGCTT+GGG | + | chr4.2:43882868-43882887 | None:intergenic | 45.0% |
| !! | AAAACTCCGGTGTTGGTTCA+TGG | - | chr4.2:43882747-43882766 | MS.gene08721:CDS | 45.0% |
| !! | AAATAAAAAAATAATATTGT+TGG | + | chr4.2:43883095-43883114 | None:intergenic | 5.0% |
| !! | AGTATTATAAATAATTTATT+GGG | - | chr4.2:43883159-43883178 | MS.gene08721:intron | 5.0% |
| !! | TAGTATTATAAATAATTTAT+TGG | - | chr4.2:43883158-43883177 | MS.gene08721:intron | 5.0% |
| GAGGGAATTTCTCAGCTGCT+AGG | + | chr4.2:43883413-43883432 | None:intergenic | 50.0% | |
| GCACAAGAAAACTCCGGTGT+TGG | - | chr4.2:43882740-43882759 | MS.gene08721:CDS | 50.0% | |
| GCAGATCAAGTGAAGCTGCA+TGG | - | chr4.2:43882594-43882613 | MS.gene08721:CDS | 50.0% | |
| GCTCTCTCATAGGGATCAAC+AGG | + | chr4.2:43882846-43882865 | None:intergenic | 50.0% | |
| GTTGGTGCACTCGTTAGTAG+CGG | - | chr4.2:43883204-43883223 | MS.gene08721:intron | 50.0% | |
| ! | ACCGGAGTTTTCTTGTGCAC+TGG | + | chr4.2:43882738-43882757 | None:intergenic | 50.0% |
| !! | ACAGGAAGCAAAGGGTACTG+TGG | + | chr4.2:43882828-43882847 | None:intergenic | 50.0% |
| CCTATGAGAGAGCCCAAGCT+CGG | - | chr4.2:43882853-43882872 | MS.gene08721:CDS | 55.0% | |
| CGAGCTTGGGCTCTCTCATA+GGG | + | chr4.2:43882855-43882874 | None:intergenic | 55.0% | |
| CTAACGAGTGCACCAACTGC+AGG | + | chr4.2:43883201-43883220 | None:intergenic | 55.0% | |
| !! | ACTCCGGTGTTGGTTCATGG+TGG | - | chr4.2:43882750-43882769 | MS.gene08721:CDS | 55.0% |
| AGAGAGCCCAAGCTCGGTTC+TGG | - | chr4.2:43882859-43882878 | MS.gene08721:CDS | 60.0% | |
| CCGAGCTTGGGCTCTCTCAT+AGG | + | chr4.2:43882856-43882875 | None:intergenic | 60.0% | |
| GAGAGCCCAAGCTCGGTTCT+GGG | - | chr4.2:43882860-43882879 | MS.gene08721:CDS | 60.0% | |
| GGTGCACTCGTTAGTAGCGG+TGG | - | chr4.2:43883207-43883226 | MS.gene08721:intron | 60.0% | |
| GTAGCGGTGGCGATGAAGAG+CGG | - | chr4.2:43883220-43883239 | MS.gene08721:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 43882591 | 43883539 | 43882591 | ID=MS.gene08721 |
| chr4.2 | mRNA | 43882591 | 43883539 | 43882591 | ID=MS.gene08721.t1;Parent=MS.gene08721 |
| chr4.2 | exon | 43883228 | 43883539 | 43883228 | ID=MS.gene08721.t1.exon1;Parent=MS.gene08721.t1 |
| chr4.2 | CDS | 43883228 | 43883539 | 43883228 | ID=cds.MS.gene08721.t1;Parent=MS.gene08721.t1 |
| chr4.2 | exon | 43882591 | 43882935 | 43882591 | ID=MS.gene08721.t1.exon2;Parent=MS.gene08721.t1 |
| chr4.2 | CDS | 43882591 | 43882935 | 43882591 | ID=cds.MS.gene08721.t1;Parent=MS.gene08721.t1 |
| Gene Sequence |
| Protein sequence |
