AlfalfaGEDB | MS.gene21053

M. sativa cultivar XinJiangDaYe / MS.gene21053

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21053.t1	AES61490.1	88.9	108	8	3	1	106	1	106	4.80E-42	180.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21053.t1	G7IAA9	88.9	108	8	3	1	106	1	106	3.5e-42	180.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene21053	0.833939	4.00E-56	-1.69E-46
MS.gene049346	MS.gene21053	0.803084	4.13E-49	-1.69E-46
MS.gene049351	MS.gene21053	0.803948	2.73E-49	-1.69E-46
MS.gene049758	MS.gene21053	0.834424	3.02E-56	-1.69E-46
MS.gene050128	MS.gene21053	0.831609	1.51E-55	-1.69E-46
MS.gene050301	MS.gene21053	0.864735	9.82E-65	-1.69E-46
MS.gene050397	MS.gene21053	0.80025	1.57E-48	-1.69E-46
MS.gene050516	MS.gene21053	0.800212	1.60E-48	-1.69E-46
MS.gene050558	MS.gene21053	0.815208	1.04E-51	-1.69E-46
MS.gene050650	MS.gene21053	0.803951	2.73E-49	-1.69E-46
MS.gene05101	MS.gene21053	0.805237	1.47E-49	-1.69E-46
MS.gene051487	MS.gene21053	0.819432	1.16E-52	-1.69E-46
MS.gene051860	MS.gene21053	0.852055	5.93E-61	-1.69E-46
MS.gene052011	MS.gene21053	-0.804381	2.22E-49	-1.69E-46
MS.gene052078	MS.gene21053	0.806735	7.12E-50	-1.69E-46
MS.gene052178	MS.gene21053	0.843686	1.20E-58	-1.69E-46
MS.gene052207	MS.gene21053	0.822193	2.70E-53	-1.69E-46
MS.gene052253	MS.gene21053	0.825407	4.77E-54	-1.69E-46
MS.gene052917	MS.gene21053	-0.806646	7.43E-50	-1.69E-46
MS.gene053074	MS.gene21053	0.826538	2.57E-54	-1.69E-46
MS.gene053204	MS.gene21053	0.813832	2.10E-51	-1.69E-46
MS.gene053836	MS.gene21053	0.846615	1.94E-59	-1.69E-46
MS.gene054010	MS.gene21053	0.895861	6.41E-76	-1.69E-46
MS.gene054011	MS.gene21053	0.902796	6.72E-79	-1.69E-46
MS.gene054012	MS.gene21053	0.896747	2.74E-76	-1.69E-46
MS.gene054013	MS.gene21053	0.89676	2.71E-76	-1.69E-46
MS.gene054183	MS.gene21053	0.813691	2.25E-51	-1.69E-46
MS.gene054184	MS.gene21053	0.801609	8.30E-49	-1.69E-46
MS.gene054185	MS.gene21053	0.810678	1.02E-50	-1.69E-46
MS.gene054214	MS.gene21053	0.831759	1.39E-55	-1.69E-46
MS.gene054473	MS.gene21053	0.833435	5.34E-56	-1.69E-46
MS.gene054618	MS.gene21053	0.829507	4.95E-55	-1.69E-46
MS.gene055277	MS.gene21053	0.820118	8.12E-53	-1.69E-46
MS.gene055303	MS.gene21053	0.808538	2.95E-50	-1.69E-46
MS.gene055390	MS.gene21053	0.820954	5.22E-53	-1.69E-46
MS.gene055422	MS.gene21053	0.846834	1.69E-59	-1.69E-46
MS.gene055428	MS.gene21053	0.834865	2.34E-56	-1.69E-46
MS.gene055604	MS.gene21053	0.86658	2.57E-65	-1.69E-46
MS.gene056191	MS.gene21053	0.84496	5.47E-59	-1.69E-46
MS.gene056902	MS.gene21053	0.856553	2.98E-62	-1.69E-46
MS.gene056931	MS.gene21053	0.805475	1.31E-49	-1.69E-46
MS.gene056933	MS.gene21053	0.801001	1.11E-48	-1.69E-46
MS.gene05702	MS.gene21053	0.805699	1.18E-49	-1.69E-46
MS.gene057121	MS.gene21053	0.833212	6.07E-56	-1.69E-46
MS.gene057245	MS.gene21053	0.824512	7.75E-54	-1.69E-46
MS.gene057469	MS.gene21053	0.833584	4.90E-56	-1.69E-46
MS.gene057752	MS.gene21053	0.801993	6.93E-49	-1.69E-46
MS.gene057779	MS.gene21053	0.802428	5.64E-49	-1.69E-46
MS.gene058002	MS.gene21053	0.832238	1.06E-55	-1.69E-46
MS.gene05811	MS.gene21053	0.860206	2.43E-63	-1.69E-46
MS.gene058168	MS.gene21053	0.80099	1.11E-48	-1.69E-46
MS.gene058484	MS.gene21053	0.816348	5.79E-52	-1.69E-46
MS.gene058660	MS.gene21053	0.805909	1.06E-49	-1.69E-46
MS.gene059006	MS.gene21053	0.800613	1.33E-48	-1.69E-46
MS.gene05907	MS.gene21053	0.845079	5.08E-59	-1.69E-46
MS.gene059228	MS.gene21053	0.815974	7.02E-52	-1.69E-46
MS.gene059294	MS.gene21053	0.857913	1.18E-62	-1.69E-46
MS.gene059420	MS.gene21053	0.800991	1.11E-48	-1.69E-46
MS.gene059518	MS.gene21053	0.848203	7.12E-60	-1.69E-46
MS.gene05984	MS.gene21053	0.840921	6.50E-58	-1.69E-46
MS.gene059974	MS.gene21053	0.811824	5.77E-51	-1.69E-46
MS.gene060005	MS.gene21053	0.813276	2.78E-51	-1.69E-46
MS.gene06037	MS.gene21053	0.850882	1.27E-60	-1.69E-46
MS.gene060559	MS.gene21053	0.80663	7.49E-50	-1.69E-46
MS.gene061049	MS.gene21053	0.845821	3.20E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21053.t1	MTR_1g084780	86.111	108	11	3	1	106	1	106	2.19e-49	152

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTCCCATGTTGAAAGTTTC+AGG	0.145284	1.4:+68590738	None:intergenic
ACTATGGTCCTGATGGTTTA+AGG	0.152607	1.4:-68590665	MS.gene21053:CDS
CCTGGGACTCTTGGTGGAAT+TGG	0.298107	1.4:-68590591	MS.gene21053:CDS
CCTACTATCCCTGGGACTCT+TGG	0.340847	1.4:-68590600	MS.gene21053:CDS
TGCATTATGTTGGTTGTCCT+TGG	0.369781	1.4:-68590816	MS.gene21053:CDS
GGTGGAATTGGAAATGGAAT+TGG	0.405856	1.4:-68590579	MS.gene21053:CDS
AGCAAAGTTACCAGGGCTGC+TGG	0.427625	1.4:+68590698	None:intergenic
ACTCTTGGTGGAATTGGAAA+TGG	0.433410	1.4:-68590585	MS.gene21053:CDS
TTCAACATGGGAGGAGGGAA+TGG	0.438827	1.4:-68590729	MS.gene21053:CDS
CCAAGAGTCCCAGGGATAGT+AGG	0.443589	1.4:+68590600	None:intergenic
ACTATCCCTGGGACTCTTGG+TGG	0.449895	1.4:-68590597	MS.gene21053:CDS
GGTGATGTTGTGCATTATGT+TGG	0.453491	1.4:-68590826	MS.gene21053:CDS
ACACCTGCAGCAAAGTTACC+AGG	0.471040	1.4:+68590690	None:intergenic
AGCCCTGGTAACTTTGCTGC+AGG	0.474026	1.4:-68590693	MS.gene21053:CDS
CCAATTCCACCAAGAGTCCC+AGG	0.481288	1.4:+68590591	None:intergenic
TTTGCTGCAGGTGTAAACTA+TGG	0.486819	1.4:-68590681	MS.gene21053:CDS
TCAGTTCATGTGGAAGGAGC+TGG	0.487807	1.4:-68590792	MS.gene21053:CDS
TTTGTTGTATTCTATGGATG+TGG	0.489015	1.4:+68590540	None:intergenic
TTCCCTGGTGGCTGTACCAG+TGG	0.504867	1.4:-68590633	MS.gene21053:CDS
ATTGGAAATGGAATTGGAGC+TGG	0.505279	1.4:-68590573	MS.gene21053:CDS
TCAACATGGGAGGAGGGAAT+GGG	0.507559	1.4:-68590728	MS.gene21053:CDS
TTGCCACTGGTACAGCCACC+AGG	0.516455	1.4:+68590630	None:intergenic
CTTGGTTCAGTTCATGTGGA+AGG	0.517140	1.4:-68590798	MS.gene21053:CDS
GAAACTTTCAACATGGGAGG+AGG	0.522497	1.4:-68590735	MS.gene21053:CDS
GTGCAAAACCTTAAACCATC+AGG	0.527769	1.4:+68590657	None:intergenic
GTTGTATTCTATGGATGTGG+TGG	0.547296	1.4:+68590543	None:intergenic
GGGATACTTCCCAGCAGCCC+TGG	0.547402	1.4:-68590708	MS.gene21053:CDS
GCAAAGTTACCAGGGCTGCT+GGG	0.564631	1.4:+68590699	None:intergenic
AGCTGGAAGGTTGCTGAAGG+AGG	0.566335	1.4:-68590775	MS.gene21053:CDS
ACTGGTACAGCCACCAGGGA+AGG	0.569138	1.4:+68590635	None:intergenic
TGTCCTTGGTTCAGTTCATG+TGG	0.588978	1.4:-68590802	MS.gene21053:CDS
GGTGAGAGTTGCAAAGGTGT+TGG	0.597999	1.4:-68590847	MS.gene21053:CDS
ACATCCTGAAACTTTCAACA+TGG	0.599933	1.4:-68590742	MS.gene21053:CDS
AAACTTTCAACATGGGAGGA+GGG	0.601619	1.4:-68590734	MS.gene21053:CDS
AGTAGGAAAAGTATTGCCAC+TGG	0.619129	1.4:+68590617	None:intergenic
GGTGTAAACTATGGTCCTGA+TGG	0.623829	1.4:-68590672	MS.gene21053:CDS
CATCCTGAAACTTTCAACAT+GGG	0.624107	1.4:-68590741	MS.gene21053:CDS
AGGAGCTGGAAGGTTGCTGA+AGG	0.626981	1.4:-68590778	MS.gene21053:CDS
CAATTCCACCAAGAGTCCCA+GGG	0.630518	1.4:+68590592	None:intergenic
CACCTGCAGCAAAGTTACCA+GGG	0.639196	1.4:+68590691	None:intergenic
CCTGAAACTTTCAACATGGG+AGG	0.659348	1.4:-68590738	MS.gene21053:CDS
CTTCCACATGAACTGAACCA+AGG	0.677096	1.4:+68590799	None:intergenic
TGCCACTGGTACAGCCACCA+GGG	0.681445	1.4:+68590631	None:intergenic
TTCATGTGGAAGGAGCTGGA+AGG	0.707170	1.4:-68590788	MS.gene21053:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACATCCTGAAACTTTCAACA+TGG	-	chr1.4:68590658-68590677	MS.gene21053:CDS	35.0%
	CATCCTGAAACTTTCAACAT+GGG	-	chr1.4:68590659-68590678	MS.gene21053:CDS	35.0%
!	AATACTTTTCCTACTATCCC+TGG	-	chr1.4:68590791-68590810	MS.gene21053:CDS	35.0%
!	ATACTTTTCCTACTATCCCT+GGG	-	chr1.4:68590792-68590811	MS.gene21053:CDS	35.0%
	AAACTTTCAACATGGGAGGA+GGG	-	chr1.4:68590666-68590685	MS.gene21053:CDS	40.0%
	ACTATGGTCCTGATGGTTTA+AGG	-	chr1.4:68590735-68590754	MS.gene21053:CDS	40.0%
	ACTCTTGGTGGAATTGGAAA+TGG	-	chr1.4:68590815-68590834	MS.gene21053:CDS	40.0%
	ATTGGAAATGGAATTGGAGC+TGG	-	chr1.4:68590827-68590846	MS.gene21053:CDS	40.0%
	GGTGATGTTGTGCATTATGT+TGG	-	chr1.4:68590574-68590593	MS.gene21053:CDS	40.0%
	GGTGGAATTGGAAATGGAAT+TGG	-	chr1.4:68590821-68590840	MS.gene21053:CDS	40.0%
	GTGCAAAACCTTAAACCATC+AGG	+	chr1.4:68590746-68590765	None:intergenic	40.0%
	TTTGCTGCAGGTGTAAACTA+TGG	-	chr1.4:68590719-68590738	MS.gene21053:CDS	40.0%
!	TGCATTATGTTGGTTGTCCT+TGG	-	chr1.4:68590584-68590603	MS.gene21053:CDS	40.0%
!!	AGTAGGAAAAGTATTGCCAC+TGG	+	chr1.4:68590786-68590805	None:intergenic	40.0%
	CCTCCCATGTTGAAAGTTTC+AGG	+	chr1.4:68590665-68590684	None:intergenic	45.0%
	CCTGAAACTTTCAACATGGG+AGG	-	chr1.4:68590662-68590681	MS.gene21053:CDS	45.0%
	CTTCCACATGAACTGAACCA+AGG	+	chr1.4:68590604-68590623	None:intergenic	45.0%
	CTTGGTTCAGTTCATGTGGA+AGG	-	chr1.4:68590602-68590621	MS.gene21053:CDS	45.0%
	GAAACTTTCAACATGGGAGG+AGG	-	chr1.4:68590665-68590684	MS.gene21053:CDS	45.0%
	GGTGTAAACTATGGTCCTGA+TGG	-	chr1.4:68590728-68590747	MS.gene21053:CDS	45.0%
	TGTCCTTGGTTCAGTTCATG+TGG	-	chr1.4:68590598-68590617	MS.gene21053:CDS	45.0%
!	TTAAGGTTTTGCACCTTCCC+TGG	-	chr1.4:68590752-68590771	MS.gene21053:CDS	45.0%
	ACACCTGCAGCAAAGTTACC+AGG	+	chr1.4:68590713-68590732	None:intergenic	50.0%
	CAATTCCACCAAGAGTCCCA+GGG	+	chr1.4:68590811-68590830	None:intergenic	50.0%
	CACCTGCAGCAAAGTTACCA+GGG	+	chr1.4:68590712-68590731	None:intergenic	50.0%
	TCAACATGGGAGGAGGGAAT+GGG	-	chr1.4:68590672-68590691	MS.gene21053:CDS	50.0%
	TCAGTTCATGTGGAAGGAGC+TGG	-	chr1.4:68590608-68590627	MS.gene21053:CDS	50.0%
	TTCAACATGGGAGGAGGGAA+TGG	-	chr1.4:68590671-68590690	MS.gene21053:CDS	50.0%
	TTCATGTGGAAGGAGCTGGA+AGG	-	chr1.4:68590612-68590631	MS.gene21053:CDS	50.0%
!	GGTGAGAGTTGCAAAGGTGT+TGG	-	chr1.4:68590553-68590572	MS.gene21053:CDS	50.0%
	ACTATCCCTGGGACTCTTGG+TGG	-	chr1.4:68590803-68590822	MS.gene21053:CDS	55.0%
	AGCAAAGTTACCAGGGCTGC+TGG	+	chr1.4:68590705-68590724	None:intergenic	55.0%
	AGCCCTGGTAACTTTGCTGC+AGG	-	chr1.4:68590707-68590726	MS.gene21053:CDS	55.0%
	AGCTGGAAGGTTGCTGAAGG+AGG	-	chr1.4:68590625-68590644	MS.gene21053:CDS	55.0%
	AGGAGCTGGAAGGTTGCTGA+AGG	-	chr1.4:68590622-68590641	MS.gene21053:CDS	55.0%
	CCAAGAGTCCCAGGGATAGT+AGG	+	chr1.4:68590803-68590822	None:intergenic	55.0%
	CCAATTCCACCAAGAGTCCC+AGG	+	chr1.4:68590812-68590831	None:intergenic	55.0%
	CCTACTATCCCTGGGACTCT+TGG	-	chr1.4:68590800-68590819	MS.gene21053:CDS	55.0%
	CCTGGGACTCTTGGTGGAAT+TGG	-	chr1.4:68590809-68590828	MS.gene21053:CDS	55.0%
	GCAAAGTTACCAGGGCTGCT+GGG	+	chr1.4:68590704-68590723	None:intergenic	55.0%
!	AGGTTTTGCACCTTCCCTGG+TGG	-	chr1.4:68590755-68590774	MS.gene21053:CDS	55.0%
	ACTGGTACAGCCACCAGGGA+AGG	+	chr1.4:68590768-68590787	None:intergenic	60.0%
	TGCCACTGGTACAGCCACCA+GGG	+	chr1.4:68590772-68590791	None:intergenic	60.0%
	TTCCCTGGTGGCTGTACCAG+TGG	-	chr1.4:68590767-68590786	MS.gene21053:CDS	60.0%
	TTGCCACTGGTACAGCCACC+AGG	+	chr1.4:68590773-68590792	None:intergenic	60.0%
	GGGATACTTCCCAGCAGCCC+TGG	-	chr1.4:68590692-68590711	MS.gene21053:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	68590551	68590871	68590551	ID=MS.gene21053
chr1.4	mRNA	68590551	68590871	68590551	ID=MS.gene21053.t1;Parent=MS.gene21053
chr1.4	exon	68590551	68590871	68590551	ID=MS.gene21053.t1.exon1;Parent=MS.gene21053.t1
chr1.4	CDS	68590551	68590871	68590551	ID=cds.MS.gene21053.t1;Parent=MS.gene21053.t1

Gene Sequence	>MS.gene21053 ATGGTGAGAGTTGCAAAGGTGTTGGTGATGTTGTGCATTATGTTGGTTGTCCTTGGTTCAGTTCATGTGGAAGGAGCTGGAAGGTTGCTGAAGGAGGAGAGTGTGAAACATCCTGAAACTTTCAACATGGGAGGAGGGAATGGGATACTTCCCAGCAGCCCTGGTAACTTTGCTGCAGGTGTAAACTATGGTCCTGATGGTTTAAGGTTTTGCACCTTCCCTGGTGGCTGTACCAGTGGCAATACTTTTCCTACTATCCCTGGGACTCTTGGTGGAATTGGAAATGGAATTGGAGCTGGATTCTCACCACCACATCCATAG
Protein sequence	>MS.gene21053.t1 MVRVAKVLVMLCIMLVVLGSVHVEGAGRLLKEESVKHPETFNMGGGNGILPSSPGNFAAGVNYGPDGLRFCTFPGGCTSGNTFPTIPGTLGGIGNGIGAGFSPPHP