AlfalfaGEDB | MS.gene26832

M. sativa cultivar XinJiangDaYe / MS.gene26832

KEGG: -
GO: GO:0003674 GO:0004857 GO:0030234 GO:0098772
PFAM: PF04043.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26832.t1	XP_003609816.1	98.1	106	2	0	1	106	77	182	5.30E-49	203.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26832.t1	F4HWQ8	44.6	101	56	0	1	101	61	161	1.3e-16	87.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26832.t1	B7FMH8	98.1	106	2	0	1	106	77	182	3.8e-49	203.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene26832	0.813534	2.44E-51	-1.69E-46
MS.gene049711	MS.gene26832	0.807616	4.63E-50	-1.69E-46
MS.gene049807	MS.gene26832	0.812104	5.01E-51	-1.69E-46
MS.gene049808	MS.gene26832	0.810786	9.70E-51	-1.69E-46
MS.gene049983	MS.gene26832	0.801805	7.57E-49	-1.69E-46
MS.gene050237	MS.gene26832	0.804725	1.88E-49	-1.69E-46
MS.gene050559	MS.gene26832	0.815712	8.03E-52	-1.69E-46
MS.gene050561	MS.gene26832	0.803943	2.74E-49	-1.69E-46
MS.gene051279	MS.gene26832	0.807645	4.57E-50	-1.69E-46
MS.gene052072	MS.gene26832	0.809745	1.63E-50	-1.69E-46
MS.gene052239	MS.gene26832	-0.818079	2.36E-52	-1.69E-46
MS.gene052463	MS.gene26832	0.803027	4.24E-49	-1.69E-46
MS.gene052516	MS.gene26832	0.834224	3.39E-56	-1.69E-46
MS.gene052519	MS.gene26832	0.821067	4.92E-53	-1.69E-46
MS.gene052793	MS.gene26832	0.803881	2.82E-49	-1.69E-46
MS.gene053006	MS.gene26832	0.824396	8.26E-54	-1.69E-46
MS.gene053226	MS.gene26832	0.801897	7.25E-49	-1.69E-46
MS.gene053272	MS.gene26832	-0.820918	5.32E-53	-1.69E-46
MS.gene05364	MS.gene26832	0.808559	2.92E-50	-1.69E-46
MS.gene053678	MS.gene26832	0.834342	3.17E-56	-1.69E-46
MS.gene054651	MS.gene26832	0.826101	3.26E-54	-1.69E-46
MS.gene055141	MS.gene26832	0.81362	2.33E-51	-1.69E-46
MS.gene05548	MS.gene26832	0.835207	1.92E-56	-1.69E-46
MS.gene055555	MS.gene26832	0.847012	1.51E-59	-1.69E-46
MS.gene055818	MS.gene26832	0.806159	9.42E-50	-1.69E-46
MS.gene05686	MS.gene26832	0.817246	3.64E-52	-1.69E-46
MS.gene05690	MS.gene26832	0.821604	3.70E-53	-1.69E-46
MS.gene057102	MS.gene26832	0.835115	2.03E-56	-1.69E-46
MS.gene057208	MS.gene26832	0.844649	6.63E-59	-1.69E-46
MS.gene057287	MS.gene26832	0.817706	2.87E-52	-1.69E-46
MS.gene057392	MS.gene26832	0.811507	6.76E-51	-1.69E-46
MS.gene057694	MS.gene26832	0.832178	1.10E-55	-1.69E-46
MS.gene057705	MS.gene26832	0.827026	1.96E-54	-1.69E-46
MS.gene058008	MS.gene26832	0.850867	1.29E-60	-1.69E-46
MS.gene058009	MS.gene26832	0.836566	8.68E-57	-1.69E-46
MS.gene058088	MS.gene26832	0.813722	2.22E-51	-1.69E-46
MS.gene058625	MS.gene26832	0.817738	2.82E-52	-1.69E-46
MS.gene058635	MS.gene26832	0.806013	1.01E-49	-1.69E-46
MS.gene058781	MS.gene26832	0.811462	6.92E-51	-1.69E-46
MS.gene058783	MS.gene26832	0.844744	6.25E-59	-1.69E-46
MS.gene05985	MS.gene26832	-0.800163	1.64E-48	-1.69E-46
MS.gene060244	MS.gene26832	0.812671	3.77E-51	-1.69E-46
MS.gene060322	MS.gene26832	0.803008	4.28E-49	-1.69E-46
MS.gene060365	MS.gene26832	0.815287	9.99E-52	-1.69E-46
MS.gene060521	MS.gene26832	0.808672	2.76E-50	-1.69E-46
MS.gene060935	MS.gene26832	0.807665	4.52E-50	-1.69E-46
MS.gene060937	MS.gene26832	0.801929	7.14E-49	-1.69E-46
MS.gene061100	MS.gene26832	0.807852	4.13E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26832.t1	MTR_4g122800	98.113	106	2	0	1	106	77	182	1.62e-73	216
MS.gene26832.t1	MTR_4g122750	55.660	106	47	0	1	106	68	173	1.87e-40	132
MS.gene26832.t1	MTR_4g122890	51.402	107	51	1	1	106	69	175	2.15e-32	111
MS.gene26832.t1	MTR_4g122760	50.000	110	49	2	1	106	69	176	9.90e-32	110
MS.gene26832.t1	MTR_4g007280	50.926	108	51	2	1	106	1	108	2.24e-28	99.8
MS.gene26832.t1	MTR_4g122780	34.426	122	64	4	1	106	37	158	5.09e-12	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26832.t1	AT1G47960	46.226	106	57	0	1	106	61	166	4.20e-28	100
MS.gene26832.t1	AT1G47960	44.554	101	56	0	1	101	61	161	1.04e-24	92.8
MS.gene26832.t1	AT3G17140	36.842	95	58	2	13	106	19	112	4.39e-15	65.9

Find 18 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTCAAGCTATTGAAGCTT+TGG	0.326895	8.1:+8086114	MS.gene26832:CDS
CACCATCTTCTGCAAACTTA+GGG	0.376121	8.1:-8086147	None:intergenic
GCACCATCTTCTGCAAACTT+AGG	0.382996	8.1:-8086148	None:intergenic
TTCTCAACCTTTGTAGCTGG+TGG	0.401916	8.1:-8086027	None:intergenic
GCTATTGAAGCTTTGGAGAA+AGG	0.407146	8.1:+8086121	MS.gene26832:CDS
CATTTCTCAACCTTTGTAGC+TGG	0.408972	8.1:-8086030	None:intergenic
CTATTGAAGCTTTGGAGAAA+GGG	0.422212	8.1:+8086122	MS.gene26832:CDS
ATTGTGAGAGTGAGTTCAAT+GGG	0.471484	8.1:+8086197	MS.gene26832:CDS
CAGCTACAAAGGTTGAGAAA+TGG	0.491946	8.1:+8086031	MS.gene26832:CDS
GACCCTAAGTTTGCAGAAGA+TGG	0.509313	8.1:+8086145	MS.gene26832:CDS
TATTGTGAGAGTGAGTTCAA+TGG	0.517229	8.1:+8086196	MS.gene26832:CDS
AGGAAAGCTTTGAGTTCTTG+TGG	0.532829	8.1:+8086058	MS.gene26832:CDS
TCCAAAGCTTCAATAGCTTG+AGG	0.539762	8.1:-8086115	None:intergenic
AAAGGTTGAGAAATGGAGCT+AGG	0.556772	8.1:+8086038	MS.gene26832:CDS
TGCAAATGATGCTGCTAATG+AGG	0.560849	8.1:+8086168	MS.gene26832:CDS
ATAGAATCCACCAGCTACAA+AGG	0.567634	8.1:+8086020	MS.gene26832:CDS
TATTGAAGCTTTGGAGAAAG+GGG	0.591796	8.1:+8086123	MS.gene26832:CDS
ATGAAAGCAAAAGCAAACGA+TGG	0.653893	8.1:+8085992	MS.gene26832:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGAAAGCAAAAGCAAACGA+TGG	+	chr8.1:8085992-8086011	MS.gene26832:CDS	35.0%
	ATTGTGAGAGTGAGTTCAAT+GGG	+	chr8.1:8086197-8086216	MS.gene26832:CDS	35.0%
	TATTGTGAGAGTGAGTTCAA+TGG	+	chr8.1:8086196-8086215	MS.gene26832:CDS	35.0%
!!	CTATTGAAGCTTTGGAGAAA+GGG	+	chr8.1:8086122-8086141	MS.gene26832:CDS	35.0%
!!	TATTGAAGCTTTGGAGAAAG+GGG	+	chr8.1:8086123-8086142	MS.gene26832:CDS	35.0%
	AAAGGTTGAGAAATGGAGCT+AGG	+	chr8.1:8086038-8086057	MS.gene26832:CDS	40.0%
	ACCTCAAGCTATTGAAGCTT+TGG	+	chr8.1:8086114-8086133	MS.gene26832:CDS	40.0%
	ATAGAATCCACCAGCTACAA+AGG	+	chr8.1:8086020-8086039	MS.gene26832:CDS	40.0%
	CACCATCTTCTGCAAACTTA+GGG	-	chr8.1:8086150-8086169	None:intergenic	40.0%
	CAGCTACAAAGGTTGAGAAA+TGG	+	chr8.1:8086031-8086050	MS.gene26832:CDS	40.0%
	CATTTCTCAACCTTTGTAGC+TGG	-	chr8.1:8086033-8086052	None:intergenic	40.0%
	TCCAAAGCTTCAATAGCTTG+AGG	-	chr8.1:8086118-8086137	None:intergenic	40.0%
	TGCAAATGATGCTGCTAATG+AGG	+	chr8.1:8086168-8086187	MS.gene26832:CDS	40.0%
!	GCATTGTTCTGTTTTGTGAG+TGG	-	chr8.1:8086229-8086248	None:intergenic	40.0%
!!	AGGAAAGCTTTGAGTTCTTG+TGG	+	chr8.1:8086058-8086077	MS.gene26832:CDS	40.0%
!!	GCTATTGAAGCTTTGGAGAA+AGG	+	chr8.1:8086121-8086140	MS.gene26832:CDS	40.0%
	GACCCTAAGTTTGCAGAAGA+TGG	+	chr8.1:8086145-8086164	MS.gene26832:CDS	45.0%
	GCACCATCTTCTGCAAACTT+AGG	-	chr8.1:8086151-8086170	None:intergenic	45.0%
	TTCTCAACCTTTGTAGCTGG+TGG	-	chr8.1:8086030-8086049	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	8085980	8086300	8085980	ID=MS.gene26832
chr8.1	mRNA	8085980	8086300	8085980	ID=MS.gene26832.t1;Parent=MS.gene26832
chr8.1	exon	8085980	8086300	8085980	ID=MS.gene26832.t1.exon1;Parent=MS.gene26832.t1
chr8.1	CDS	8085980	8086300	8085980	ID=cds.MS.gene26832.t1;Parent=MS.gene26832.t1

Gene Sequence	>MS.gene26832 ATGGTGAAAGTGATGAAAGCAAAAGCAAACGATGGATTAAATAGAATCCACCAGCTACAAAGGTTGAGAAATGGAGCTAGGAAAGCTTTGAGTTCTTGTGGTGACAAATACAGAGCAATTTTGATAGCTGATATACCTCAAGCTATTGAAGCTTTGGAGAAAGGGGACCCTAAGTTTGCAGAAGATGGTGCAAATGATGCTGCTAATGAGGCTACTTATTGTGAGAGTGAGTTCAATGGGAAATCTCCACTCACAAAACAGAACAATGCTATGCATGATGTTTCAGCTGTTACATCATCTATTGTTAGACAATTACTCTAG
Protein sequence	>MS.gene26832.t1 MVKVMKAKANDGLNRIHQLQRLRNGARKALSSCGDKYRAILIADIPQAIEALEKGDPKFAEDGANDAANEATYCESEFNGKSPLTKQNNAMHDVSAVTSSIVRQLL