AlfalfaGEDB | MS.gene27318

M. sativa cultivar XinJiangDaYe / MS.gene27318

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27318.t1	XP_024638160.1	63.9	119	31	3	1	117	1	109	1.10E-18	102.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27318.t1	I3SX70	63.9	119	31	3	1	117	1	109	7.8e-19	102.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene27318	0.802148	6.44E-49	-1.69E-46
MS.gene049283	MS.gene27318	0.842414	2.62E-58	-1.69E-46
MS.gene049983	MS.gene27318	0.826461	2.68E-54	-1.69E-46
MS.gene04998	MS.gene27318	0.8314	1.71E-55	-1.69E-46
MS.gene051676	MS.gene27318	0.823592	1.28E-53	-1.69E-46
MS.gene051975	MS.gene27318	0.822793	1.96E-53	-1.69E-46
MS.gene052434	MS.gene27318	0.818131	2.30E-52	-1.69E-46
MS.gene052493	MS.gene27318	0.805383	1.37E-49	-1.69E-46
MS.gene052533	MS.gene27318	0.806603	7.59E-50	-1.69E-46
MS.gene052685	MS.gene27318	0.807511	4.88E-50	-1.69E-46
MS.gene052818	MS.gene27318	-0.813068	3.09E-51	-1.69E-46
MS.gene053298	MS.gene27318	0.992005	9.19E-191	-1.69E-46
MS.gene053340	MS.gene27318	0.827726	1.33E-54	-1.69E-46
MS.gene053786	MS.gene27318	0.805955	1.04E-49	-1.69E-46
MS.gene053963	MS.gene27318	0.880064	7.47E-70	-1.69E-46
MS.gene054222	MS.gene27318	0.852239	5.26E-61	-1.69E-46
MS.gene054479	MS.gene27318	0.816169	6.35E-52	-1.69E-46
MS.gene05469	MS.gene27318	0.814757	1.31E-51	-1.69E-46
MS.gene054892	MS.gene27318	0.832167	1.10E-55	-1.69E-46
MS.gene054893	MS.gene27318	0.832822	7.59E-56	-1.69E-46
MS.gene05517	MS.gene27318	0.800088	1.70E-48	-1.69E-46
MS.gene055513	MS.gene27318	0.808139	3.59E-50	-1.69E-46
MS.gene055603	MS.gene27318	0.808625	2.82E-50	-1.69E-46
MS.gene05636	MS.gene27318	0.803243	3.83E-49	-1.69E-46
MS.gene056403	MS.gene27318	0.82432	8.60E-54	-1.69E-46
MS.gene056605	MS.gene27318	0.813426	2.57E-51	-1.69E-46
MS.gene056776	MS.gene27318	0.806332	8.66E-50	-1.69E-46
MS.gene056779	MS.gene27318	0.806324	8.69E-50	-1.69E-46
MS.gene056786	MS.gene27318	0.803776	2.97E-49	-1.69E-46
MS.gene056986	MS.gene27318	0.844754	6.21E-59	-1.69E-46
MS.gene057102	MS.gene27318	0.825481	4.58E-54	-1.69E-46
MS.gene057155	MS.gene27318	0.856488	3.11E-62	-1.69E-46
MS.gene057622	MS.gene27318	0.815743	7.91E-52	-1.69E-46
MS.gene057999	MS.gene27318	0.838665	2.51E-57	-1.69E-46
MS.gene058159	MS.gene27318	0.815845	7.50E-52	-1.69E-46
MS.gene058279	MS.gene27318	0.801075	1.07E-48	-1.69E-46
MS.gene058429	MS.gene27318	0.804736	1.87E-49	-1.69E-46
MS.gene058592	MS.gene27318	0.804204	2.42E-49	-1.69E-46
MS.gene058897	MS.gene27318	0.802573	5.26E-49	-1.69E-46
MS.gene05952	MS.gene27318	0.811868	5.64E-51	-1.69E-46
MS.gene05985	MS.gene27318	-0.821585	3.73E-53	-1.69E-46
MS.gene060012	MS.gene27318	0.815507	8.92E-52	-1.69E-46
MS.gene06011	MS.gene27318	0.822452	2.35E-53	-1.69E-46
MS.gene060259	MS.gene27318	0.832901	7.26E-56	-1.69E-46
MS.gene060412	MS.gene27318	0.832838	7.52E-56	-1.69E-46
MS.gene060851	MS.gene27318	0.801704	7.94E-49	-1.69E-46
MS.gene060852	MS.gene27318	0.800977	1.12E-48	-1.69E-46
MS.gene060932	MS.gene27318	0.808142	3.58E-50	-1.69E-46
MS.gene060937	MS.gene27318	0.809734	1.63E-50	-1.69E-46
MS.gene061047	MS.gene27318	0.80522	1.48E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 13 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATTGAGTACTACTCTTTA+TGG	0.120660	1.2:+2436077	None:intergenic
TATTGAGTACTACTCTTTAT+GGG	0.330343	1.2:+2436078	None:intergenic
CAAGACACAGACACACCTTT+TGG	0.331421	1.2:+2436132	None:intergenic
GAGATTCAACTTGTTCCAAA+AGG	0.436800	1.2:-2436147	MS.gene27318:CDS
TCGTAAGATTGTTGTGAAGA+TGG	0.478593	1.2:-2436295	MS.gene27318:CDS
CTTGTAGAAGCAGCAGAGCT+AGG	0.495428	1.2:-2436113	MS.gene27318:CDS
GGTTCTATGAAGAAAGAAGA+AGG	0.541223	1.2:-2436228	MS.gene27318:CDS
ATCAGAAGTAGAAGCATGCA+TGG	0.580042	1.2:-2436193	MS.gene27318:CDS
TCACAACAACAAGAAGAAGA+AGG	0.587622	1.2:-2436249	MS.gene27318:CDS
ATGCATGGTTAGAAAAGAAG+AGG	0.601267	1.2:-2436178	MS.gene27318:CDS
TGCATGGTTAGAAAAGAAGA+GGG	0.637344	1.2:-2436177	MS.gene27318:CDS
ATCTTCACAACAATCTTACG+AGG	0.649549	1.2:+2436297	None:intergenic
ATGGTTAGAAAAGAAGAGGG+AGG	0.656282	1.2:-2436174	MS.gene27318:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAAAAACAAAGGAAATTT+GGG	-	chr1.2:2436091-2436110	MS.gene27318:CDS	20.0%
!	CACAAAAAACAAAGGAAATT+TGG	-	chr1.2:2436090-2436109	MS.gene27318:CDS	25.0%
!	TAAGAAACCACAAAAAACAA+AGG	-	chr1.2:2436082-2436101	MS.gene27318:CDS	25.0%
!	TATTGAGTACTACTCTTTAT+GGG	+	chr1.2:2436410-2436429	None:intergenic	25.0%
!	TTATTGAGTACTACTCTTTA+TGG	+	chr1.2:2436411-2436430	None:intergenic	25.0%
!!!	CAAATTTCCTTTGTTTTTTG+TGG	+	chr1.2:2436092-2436111	None:intergenic	25.0%
	ATCTTCACAACAATCTTACG+AGG	+	chr1.2:2436191-2436210	None:intergenic	35.0%
	ATGCATGGTTAGAAAAGAAG+AGG	-	chr1.2:2436307-2436326	MS.gene27318:CDS	35.0%
	GGTTCTATGAAGAAAGAAGA+AGG	-	chr1.2:2436257-2436276	MS.gene27318:CDS	35.0%
	TCACAACAACAAGAAGAAGA+AGG	-	chr1.2:2436236-2436255	MS.gene27318:CDS	35.0%
	TCGTAAGATTGTTGTGAAGA+TGG	-	chr1.2:2436190-2436209	MS.gene27318:CDS	35.0%
	TGCATGGTTAGAAAAGAAGA+GGG	-	chr1.2:2436308-2436327	MS.gene27318:CDS	35.0%
!	GAGATTCAACTTGTTCCAAA+AGG	-	chr1.2:2436338-2436357	MS.gene27318:CDS	35.0%
	ATGGTTAGAAAAGAAGAGGG+AGG	-	chr1.2:2436311-2436330	MS.gene27318:CDS	40.0%
!	ATCAGAAGTAGAAGCATGCA+TGG	-	chr1.2:2436292-2436311	MS.gene27318:CDS	40.0%
	CCTCAACCTACCAGAAAAAG+AGG	-	chr1.2:2436161-2436180	MS.gene27318:CDS	45.0%
!	ACGAGGTCTACCTCTTTTTC+TGG	+	chr1.2:2436174-2436193	None:intergenic	45.0%
!	CAAGACACAGACACACCTTT+TGG	+	chr1.2:2436356-2436375	None:intergenic	45.0%
!!	CCTCTTTTTCTGGTAGGTTG+AGG	+	chr1.2:2436164-2436183	None:intergenic	45.0%
!!	GGTCTACCTCTTTTTCTGGT+AGG	+	chr1.2:2436170-2436189	None:intergenic	45.0%
	CTTGTAGAAGCAGCAGAGCT+AGG	-	chr1.2:2436372-2436391	MS.gene27318:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	2436077	2436430	2436077	ID=MS.gene27318
chr1.2	mRNA	2436077	2436430	2436077	ID=MS.gene27318.t1;Parent=MS.gene27318
chr1.2	exon	2436077	2436430	2436077	ID=MS.gene27318.t1.exon1;Parent=MS.gene27318.t1
chr1.2	CDS	2436077	2436430	2436077	ID=cds.MS.gene27318.t1;Parent=MS.gene27318.t1

Gene Sequence	>MS.gene27318 ATGGGTAAGAAACCACAAAAAACAAAGGAAATTTGGGCAGCAATAGCTGAAGCATCATCAACAATAGATGAAAATCAAGAACAACCTCAACCTACCAGAAAAAGAGGTAGACCTCGTAAGATTGTTGTGAAGATGGAAAGTTCAGAAGAAAAAGCAGCTTCACAACAACAAGAAGAAGAAGGTTCTATGAAGAAAGAAGAAGGAACAAAAACATCATCAGAAGTAGAAGCATGCATGGTTAGAAAAGAAGAGGGAGGTGAAGAGATTCAACTTGTTCCAAAAGGTGTGTCTGTGTCTTGTAGAAGCAGCAGAGCTAGGCGTAAAAGCAAACCCATAAAGAGTAGTACTCAATAA
Protein sequence	>MS.gene27318.t1 MGKKPQKTKEIWAAIAEASSTIDENQEQPQPTRKRGRPRKIVVKMESSEEKAASQQQEEEGSMKKEEGTKTSSEVEACMVRKEEGGEEIQLVPKGVSVSCRSSRARRKSKPIKSSTQ