Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28488.t1 | RHN45016.1 | 88.1 | 734 | 66 | 2 | 1 | 718 | 1 | 729 | 2.10E-181 | 646 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28488.t1 | Q9Y222 | 25.8 | 256 | 173 | 7 | 439 | 689 | 156 | 399 | 4.8e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28488.t1 | A0A396GV57 | 88.1 | 734 | 66 | 2 | 1 | 718 | 1 | 729 | 1.5e-181 | 646.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28488.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene28488 | 0.866647 | 2.45E-65 | -1.69E-46 |
| MS.gene049159 | MS.gene28488 | 0.857262 | 1.84E-62 | -1.69E-46 |
| MS.gene049368 | MS.gene28488 | 0.844934 | 5.56E-59 | -1.69E-46 |
| MS.gene049501 | MS.gene28488 | 0.813475 | 2.51E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene28488 | 0.801253 | 9.83E-49 | -1.69E-46 |
| MS.gene050384 | MS.gene28488 | 0.820118 | 8.12E-53 | -1.69E-46 |
| MS.gene050386 | MS.gene28488 | 0.821579 | 3.75E-53 | -1.69E-46 |
| MS.gene050551 | MS.gene28488 | 0.822491 | 2.30E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene28488 | 0.808971 | 2.38E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene28488 | 0.869731 | 2.49E-66 | -1.69E-46 |
| MS.gene050737 | MS.gene28488 | 0.827004 | 1.99E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene28488 | 0.823916 | 1.07E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene28488 | 0.81023 | 1.28E-50 | -1.69E-46 |
| MS.gene050941 | MS.gene28488 | 0.854329 | 1.32E-61 | -1.69E-46 |
| MS.gene051003 | MS.gene28488 | 0.818467 | 1.93E-52 | -1.69E-46 |
| MS.gene051102 | MS.gene28488 | 0.807719 | 4.41E-50 | -1.69E-46 |
| MS.gene051180 | MS.gene28488 | 0.800855 | 1.18E-48 | -1.69E-46 |
| MS.gene051328 | MS.gene28488 | 0.847456 | 1.14E-59 | -1.69E-46 |
| MS.gene051363 | MS.gene28488 | 0.800356 | 1.50E-48 | -1.69E-46 |
| MS.gene051365 | MS.gene28488 | 0.804932 | 1.70E-49 | -1.69E-46 |
| MS.gene051452 | MS.gene28488 | 0.802675 | 5.01E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene28488 | 0.801848 | 7.42E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene28488 | 0.814473 | 1.51E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene28488 | 0.804217 | 2.40E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene28488 | 0.821008 | 5.07E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene28488 | 0.809422 | 1.91E-50 | -1.69E-46 |
| MS.gene052397 | MS.gene28488 | 0.803919 | 2.77E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene28488 | 0.816582 | 5.13E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene28488 | 0.852435 | 4.63E-61 | -1.69E-46 |
| MS.gene052518 | MS.gene28488 | 0.829248 | 5.72E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene28488 | 0.804136 | 2.50E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene28488 | 0.873579 | 1.32E-67 | -1.69E-46 |
| MS.gene052896 | MS.gene28488 | 0.871744 | 5.41E-67 | -1.69E-46 |
| MS.gene053008 | MS.gene28488 | 0.873401 | 1.51E-67 | -1.69E-46 |
| MS.gene053381 | MS.gene28488 | 0.839583 | 1.45E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene28488 | 0.818199 | 2.22E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene28488 | 0.85615 | 3.91E-62 | -1.69E-46 |
| MS.gene054823 | MS.gene28488 | 0.820357 | 7.16E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene28488 | 0.826163 | 3.15E-54 | -1.69E-46 |
| MS.gene055310 | MS.gene28488 | 0.820901 | 5.37E-53 | -1.69E-46 |
| MS.gene055311 | MS.gene28488 | 0.817202 | 3.73E-52 | -1.69E-46 |
| MS.gene055313 | MS.gene28488 | 0.823597 | 1.27E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene28488 | 0.808212 | 3.46E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene28488 | 0.823995 | 1.03E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene28488 | 0.824492 | 7.84E-54 | -1.69E-46 |
| MS.gene055512 | MS.gene28488 | 0.807127 | 5.88E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene28488 | 0.853534 | 2.24E-61 | -1.69E-46 |
| MS.gene055818 | MS.gene28488 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene28488 | 0.809382 | 1.95E-50 | -1.69E-46 |
| MS.gene055979 | MS.gene28488 | 0.84303 | 1.80E-58 | -1.69E-46 |
| MS.gene056181 | MS.gene28488 | 0.830565 | 2.73E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene28488 | 0.817283 | 3.57E-52 | -1.69E-46 |
| MS.gene056831 | MS.gene28488 | 0.836778 | 7.66E-57 | -1.69E-46 |
| MS.gene057034 | MS.gene28488 | 0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene28488 | 0.800557 | 1.36E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene28488 | 0.800147 | 1.65E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene28488 | 0.839412 | 1.61E-57 | -1.69E-46 |
| MS.gene058010 | MS.gene28488 | 0.801067 | 1.07E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene28488 | 0.807016 | 6.21E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene28488 | 0.81017 | 1.32E-50 | -1.69E-46 |
| MS.gene058646 | MS.gene28488 | 0.838326 | 3.07E-57 | -1.69E-46 |
| MS.gene058654 | MS.gene28488 | 0.806215 | 9.17E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene28488 | 0.81401 | 1.92E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene28488 | 0.800765 | 1.24E-48 | -1.69E-46 |
| MS.gene059283 | MS.gene28488 | 0.838184 | 3.34E-57 | -1.69E-46 |
| MS.gene059842 | MS.gene28488 | 0.866721 | 2.32E-65 | -1.69E-46 |
| MS.gene06010 | MS.gene28488 | 0.814674 | 1.37E-51 | -1.69E-46 |
| MS.gene060296 | MS.gene28488 | 0.800877 | 1.17E-48 | -1.69E-46 |
| MS.gene060637 | MS.gene28488 | 0.814911 | 1.21E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene28488 | 0.821915 | 3.13E-53 | -1.69E-46 |
| MS.gene060870 | MS.gene28488 | 0.804806 | 1.81E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene28488 | 0.822277 | 2.58E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene28488 | 0.810046 | 1.40E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene28488 | 0.802464 | 5.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28488.t1 | MTR_8g077990 | 87.891 | 735 | 30 | 5 | 1 | 718 | 1 | 693 | 0.0 | 1117 |
| MS.gene28488.t1 | MTR_1g076600 | 95.050 | 101 | 5 | 0 | 618 | 718 | 55 | 155 | 1.88e-66 | 216 |
| MS.gene28488.t1 | MTR_7g029435 | 93.478 | 92 | 6 | 0 | 627 | 718 | 1 | 92 | 4.58e-57 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28488.t1 | AT5G41020 | 55.124 | 283 | 126 | 1 | 433 | 715 | 302 | 583 | 1.69e-112 | 352 |
Find 125 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATAAATTCAAGCTCTTC+AGG | 0.254261 | 8.4:-28538191 | None:intergenic |
| ATCATCATCTTCACATTCAT+TGG | 0.267885 | 8.4:-28537460 | None:intergenic |
| AACAAATGATCCACCATATT+GGG | 0.286872 | 8.4:+28538675 | MS.gene28488:CDS |
| ACATTGCTTTCGGACATATA+TGG | 0.292466 | 8.4:-28536718 | None:intergenic |
| TTGATCTTCTCATCTTCTTC+GGG | 0.322099 | 8.4:-28537972 | None:intergenic |
| TTTGATCTTCTCATCTTCTT+CGG | 0.324153 | 8.4:-28537973 | None:intergenic |
| GAACAAATGATCCACCATAT+TGG | 0.329038 | 8.4:+28538674 | MS.gene28488:CDS |
| CTATATAGATTCTCATGGTT+TGG | 0.339430 | 8.4:+28538010 | MS.gene28488:CDS |
| TATATAGATTCTCATGGTTT+GGG | 0.352407 | 8.4:+28538011 | MS.gene28488:CDS |
| TCTCTATCTTTCAACAAGTT+GGG | 0.365789 | 8.4:-28538755 | None:intergenic |
| CTCTCTATCTTTCAACAAGT+TGG | 0.365952 | 8.4:-28538756 | None:intergenic |
| CAGATCAGTTACATTGCTTT+CGG | 0.367004 | 8.4:-28536728 | None:intergenic |
| TTATGTGTGCTTTCTTCACT+TGG | 0.367181 | 8.4:-28537225 | None:intergenic |
| TAGCCCTCTCAAGCAGATCT+AGG | 0.372193 | 8.4:-28538376 | None:intergenic |
| GATAGACGCTTTATATGCTT+TGG | 0.375237 | 8.4:+28538574 | MS.gene28488:CDS |
| ACTCCTAACTCTGCACATAA+TGG | 0.384709 | 8.4:+28537861 | MS.gene28488:CDS |
| GTTCCATTATGTGCAGAGTT+AGG | 0.391649 | 8.4:-28537864 | None:intergenic |
| CTGATCTGCCTTTGATCAAA+AGG | 0.395638 | 8.4:+28536743 | MS.gene28488:CDS |
| TGGCGGCATGCTCCCCAATA+TGG | 0.396320 | 8.4:-28538688 | None:intergenic |
| GGGAGACGAAGGTCTTGATA+TGG | 0.410993 | 8.4:+28538031 | MS.gene28488:CDS |
| TTCCAACAGCCGCTAACCTC+AGG | 0.416232 | 8.4:-28538074 | None:intergenic |
| AGAAGCAATGAAGGTGATAA+TGG | 0.417384 | 8.4:+28536979 | MS.gene28488:CDS |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.4:-28537708 | None:intergenic |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.4:-28537840 | None:intergenic |
| GAGAGCAGTAGCTGACGCTT+TGG | 0.427556 | 8.4:+28538244 | MS.gene28488:CDS |
| TGCTCATACTTTGTTAGAAA+AGG | 0.429545 | 8.4:+28538148 | MS.gene28488:CDS |
| GTTGCGAGATAATATTTGTT+GGG | 0.434220 | 8.4:+28538430 | MS.gene28488:CDS |
| TCATCATCTTCACATTCATT+GGG | 0.442787 | 8.4:-28537459 | None:intergenic |
| AGAGCAGTAGCTGACGCTTT+GGG | 0.446113 | 8.4:+28538245 | MS.gene28488:CDS |
| GCTGCATGGAGGAGGTAGAC+TGG | 0.446516 | 8.4:+28538603 | MS.gene28488:CDS |
| GTCACTGATGTTAATGATCA+AGG | 0.447087 | 8.4:+28537768 | MS.gene28488:CDS |
| CTAAACGCAAACAAGTAACT+TGG | 0.447239 | 8.4:+28537889 | MS.gene28488:CDS |
| TATTCTTCTTCGTCTTGCCT+TGG | 0.449662 | 8.4:-28537536 | None:intergenic |
| CGCCAAGTCATTCATTGAGC+AGG | 0.451714 | 8.4:+28538706 | MS.gene28488:CDS |
| CATTGGTTGCTAGTGGTGAT+TGG | 0.452021 | 8.4:+28538528 | MS.gene28488:CDS |
| AATATTTGTTGGGAGGCAAT+TGG | 0.463198 | 8.4:+28538440 | MS.gene28488:CDS |
| GTCACTGAAGTTAATGATCA+AGG | 0.467385 | 8.4:+28537636 | MS.gene28488:CDS |
| CGTCGATACACACTCTCATA+AGG | 0.471062 | 8.4:-28538125 | None:intergenic |
| ACACTCTCATAAGGCCTCTC+AGG | 0.477449 | 8.4:-28538116 | None:intergenic |
| AAGAAGCACAAACTAATTGA+AGG | 0.478439 | 8.4:+28537306 | MS.gene28488:CDS |
| ACTAGCTCCATGTTGCTCAT+AGG | 0.479373 | 8.4:-28538219 | None:intergenic |
| TGCTTTGGATGCTTGCTGCA+TGG | 0.479442 | 8.4:+28538589 | MS.gene28488:CDS |
| AAAAGGAGGGAAGGTGTAAG+TGG | 0.481580 | 8.4:+28538165 | MS.gene28488:CDS |
| TAGAAGCAATGGAGGTGAAG+AGG | 0.481860 | 8.4:+28537356 | MS.gene28488:CDS |
| ACGGCTTATTGCGTGGGAAA+AGG | 0.484774 | 8.4:+28537943 | MS.gene28488:CDS |
| TGTTGCGAGATAATATTTGT+TGG | 0.489321 | 8.4:+28538429 | MS.gene28488:CDS |
| GGATGCTTGCTGCATGGAGG+AGG | 0.492182 | 8.4:+28538595 | MS.gene28488:CDS |
| ACCCTGAGGTTAGCGGCTGT+TGG | 0.493985 | 8.4:+28538072 | MS.gene28488:CDS |
| AAGTGAAGAAAGCACACATA+AGG | 0.495960 | 8.4:+28537227 | MS.gene28488:CDS |
| AAGTGAAGAAAGTAAACATA+AGG | 0.498172 | 8.4:+28537419 | MS.gene28488:CDS |
| TGAAGGTGATAATGGCGAGC+AGG | 0.499793 | 8.4:+28536987 | MS.gene28488:CDS |
| GAGTGACGAAAGCAAACATA+AGG | 0.503590 | 8.4:+28537038 | MS.gene28488:CDS |
| CTCTATCTTTCAACAAGTTG+GGG | 0.504361 | 8.4:-28538754 | None:intergenic |
| AGAAACTAATTGAGGAAGGT+GGG | 0.510841 | 8.4:+28537505 | MS.gene28488:CDS |
| TGTAATGACTTTAGAAGCAA+TGG | 0.511180 | 8.4:+28537345 | MS.gene28488:CDS |
| AAAAGCAATAAAAGTGAAGA+TGG | 0.511576 | 8.4:+28536787 | MS.gene28488:CDS |
| CTGCTCAATGAATGACTTGG+CGG | 0.516982 | 8.4:-28538705 | None:intergenic |
| AAGAAACTAATTGAGGAAGG+TGG | 0.519932 | 8.4:+28537504 | MS.gene28488:CDS |
| AATCTACTTGAGCATAGGTC+TGG | 0.523977 | 8.4:+28538629 | MS.gene28488:CDS |
| CTGCATGGAGGAGGTAGACT+GGG | 0.526890 | 8.4:+28538604 | MS.gene28488:CDS |
| TCAGCATCACCTTCATTGCT+AGG | 0.528214 | 8.4:-28537732 | None:intergenic |
| TCATACTTTGTTAGAAAAGG+AGG | 0.529507 | 8.4:+28538151 | MS.gene28488:CDS |
| AATGAAGAAGAAACTAATTG+AGG | 0.530342 | 8.4:+28537497 | MS.gene28488:CDS |
| TTTGACTATATAGATTCTCA+TGG | 0.532297 | 8.4:+28538005 | MS.gene28488:CDS |
| AAGAAGCGGAAGCTAATTGA+AGG | 0.534553 | 8.4:+28536928 | MS.gene28488:CDS |
| CATATATGGATCGCCTCCAT+TGG | 0.534788 | 8.4:-28536704 | None:intergenic |
| GGAGGCAATTGGTCACAAGT+TGG | 0.536501 | 8.4:+28538451 | MS.gene28488:CDS |
| AAAAGTGAAGATGGCGAGCA+AGG | 0.538269 | 8.4:+28536796 | MS.gene28488:CDS |
| ATAGGTCTGGTGATGTGTGC+AGG | 0.541652 | 8.4:+28538642 | MS.gene28488:CDS |
| ATGAGCAACATGGAGCTAGT+TGG | 0.543870 | 8.4:+28538222 | MS.gene28488:CDS |
| AATCACAGGTTTGTTGTCAA+AGG | 0.550299 | 8.4:-28538780 | None:intergenic |
| TTTGGGTAAAAGTCGAGCTC+AGG | 0.550583 | 8.4:+28538262 | MS.gene28488:CDS |
| AGTAACTTGGGCTGATGAAG+TGG | 0.552578 | 8.4:+28537902 | MS.gene28488:CDS |
| AAGAAGAAACTAATTGAGGA+AGG | 0.552685 | 8.4:+28537501 | MS.gene28488:CDS |
| AATGAAACTCCTAGCAATGA+AGG | 0.554019 | 8.4:+28537723 | MS.gene28488:CDS |
| AAGAGAACTGAAGGCGAAGA+GGG | 0.556027 | 8.4:+28536676 | MS.gene28488:CDS |
| GACGGCTTTAGAAGCAATGA+AGG | 0.559488 | 8.4:+28536970 | MS.gene28488:CDS |
| TGTTGTGACGGCTTATTGCG+TGG | 0.561335 | 8.4:+28537936 | MS.gene28488:CDS |
| TGAGCAATTAACATCAACAT+TGG | 0.568371 | 8.4:+28538511 | MS.gene28488:CDS |
| TAAACCTAGATCTGCTTGAG+AGG | 0.570723 | 8.4:+28538372 | MS.gene28488:CDS |
| AGAAGCAATGGAGGTGAAGA+GGG | 0.572295 | 8.4:+28537357 | MS.gene28488:CDS |
| TAGAAACCTCAACAAATCAC+AGG | 0.579568 | 8.4:-28538794 | None:intergenic |
| GAAGGTGATAATGGCGAGCA+GGG | 0.580967 | 8.4:+28536988 | MS.gene28488:CDS |
| CATACTTTGTTAGAAAAGGA+GGG | 0.581182 | 8.4:+28538152 | MS.gene28488:CDS |
| ACATCAACATTGGTTGCTAG+TGG | 0.583463 | 8.4:+28538521 | MS.gene28488:CDS |
| AACAAACCTGTGATTTGTTG+AGG | 0.586181 | 8.4:+28538788 | MS.gene28488:CDS |
| GCGAGATAATATTTGTTGGG+AGG | 0.587211 | 8.4:+28538433 | MS.gene28488:CDS |
| CATATAAAGCGTCTATCAAG+CGG | 0.589919 | 8.4:-28538568 | None:intergenic |
| GGCGAAGAGGGTGAAACCAA+TGG | 0.590103 | 8.4:+28536688 | MS.gene28488:CDS |
| GAGGAGAAGTTATGTTGTGA+CGG | 0.591020 | 8.4:+28537924 | MS.gene28488:CDS |
| AAATTACACCCTGAGGTTAG+CGG | 0.591381 | 8.4:+28538065 | MS.gene28488:CDS |
| CTTTGTTAGAAAAGGAGGGA+AGG | 0.592917 | 8.4:+28538156 | MS.gene28488:CDS |
| CGGCATGCTCCCCAATATGG+TGG | 0.593209 | 8.4:-28538685 | None:intergenic |
| AGAAGCAATGATGATGAAGA+TGG | 0.594055 | 8.4:+28537165 | MS.gene28488:CDS |
| AAACCTAGATCTGCTTGAGA+GGG | 0.602034 | 8.4:+28538373 | MS.gene28488:CDS |
| TCCAACAGCCGCTAACCTCA+GGG | 0.602654 | 8.4:-28538073 | None:intergenic |
| TAAACGCAAACAAGTAACTT+GGG | 0.603412 | 8.4:+28537890 | MS.gene28488:CDS |
| CAACTTGTTGAAAGATAGAG+AGG | 0.613256 | 8.4:+28538757 | MS.gene28488:CDS |
| AACTTGGGCTGATGAAGTGG+AGG | 0.617904 | 8.4:+28537905 | MS.gene28488:CDS |
| GGGACAATCTACTTGAGCAT+AGG | 0.618965 | 8.4:+28538624 | MS.gene28488:CDS |
| CAAGAGAACTGAAGGCGAAG+AGG | 0.619279 | 8.4:+28536675 | MS.gene28488:CDS |
| GAAGGAAACACACTTGAGGA+AGG | 0.622295 | 8.4:+28536946 | MS.gene28488:CDS |
| ACAAATGATCCACCATATTG+GGG | 0.622830 | 8.4:+28538676 | MS.gene28488:CDS |
| TCTCATGGTTTGGGAGACGA+AGG | 0.623440 | 8.4:+28538020 | MS.gene28488:CDS |
| AACCTGCTCAATGAATGACT+TGG | 0.625939 | 8.4:-28538708 | None:intergenic |
| GTGATGTGTGCAGGAAACGA+TGG | 0.634053 | 8.4:+28538651 | MS.gene28488:CDS |
| GAAGGTGGGAATCGTGACCA+AGG | 0.636683 | 8.4:+28537519 | MS.gene28488:CDS |
| CTGCCTTTGATCAAAAGGAA+TGG | 0.637174 | 8.4:+28536748 | MS.gene28488:CDS |
| GAAAAGACCTATGAGCAACA+TGG | 0.638568 | 8.4:+28538212 | MS.gene28488:CDS |
| GTTGTGACGGCTTATTGCGT+GGG | 0.642851 | 8.4:+28537937 | MS.gene28488:CDS |
| TTTGGATGCTTGCTGCATGG+AGG | 0.643384 | 8.4:+28538592 | MS.gene28488:CDS |
| TGATGTGTGCAGGAAACGAT+GGG | 0.644218 | 8.4:+28538652 | MS.gene28488:CDS |
| AATGAATGTGAAGATGATGA+TGG | 0.645642 | 8.4:+28537462 | MS.gene28488:CDS |
| AATTGAAGGAAACACACTGA+AGG | 0.653637 | 8.4:+28537128 | MS.gene28488:CDS |
| AATGACTTTAGAAGCAATGG+AGG | 0.658645 | 8.4:+28537348 | MS.gene28488:CDS |
| AACACACTTGAGGAAGGTGA+CGG | 0.661228 | 8.4:+28536952 | MS.gene28488:CDS |
| AAATGTCAAATTACACCCTG+AGG | 0.666420 | 8.4:+28538058 | MS.gene28488:CDS |
| AAAAGGACATTGGTCCCAAG+AGG | 0.666914 | 8.4:+28538325 | MS.gene28488:CDS |
| AATTGAAGGAAACACACTTG+AGG | 0.669341 | 8.4:+28536942 | MS.gene28488:CDS |
| TGTGAAGATGATGATGGCCA+AGG | 0.683186 | 8.4:+28537468 | MS.gene28488:CDS |
| GAGCTCAGGTAAAAGATGCG+TGG | 0.687675 | 8.4:+28538276 | MS.gene28488:CDS |
| GAAGAGGGTGAAACCAATGG+AGG | 0.700449 | 8.4:+28536691 | MS.gene28488:CDS |
| GAATGTGAAGCTGATGACCA+AGG | 0.711673 | 8.4:+28537084 | MS.gene28488:CDS |
| AATGAATGTGAAGATGACCA+AGG | 0.719006 | 8.4:+28537270 | MS.gene28488:CDS |
| GAATGTGAAGATGATGACCA+AGG | 0.731540 | 8.4:+28536892 | MS.gene28488:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATACCAAAGCAAAAAA+AGG | + | chr8.4:28538308-28538327 | MS.gene28488:CDS | 20.0% |
| !!! | AATGATTTTAATAGCAAAGA+AGG | + | chr8.4:28537591-28537610 | MS.gene28488:CDS | 20.0% |
| ! | AAAAGCAATAAAAGTGAAGA+TGG | + | chr8.4:28536787-28536806 | MS.gene28488:CDS | 25.0% |
| ! | AAGTGAAGAAAGTAAACATA+AGG | + | chr8.4:28537419-28537438 | MS.gene28488:CDS | 25.0% |
| ! | AATGAAGAAGAAACTAATTG+AGG | + | chr8.4:28537497-28537516 | MS.gene28488:CDS | 25.0% |
| ! | ATGAAAAAGAAGCTAATTGA+AGG | + | chr8.4:28537114-28537133 | MS.gene28488:CDS | 25.0% |
| ! | CCTTATAAAAAATCACAACA+TGG | + | chr8.4:28538404-28538423 | MS.gene28488:CDS | 25.0% |
| ! | TATATAGATTCTCATGGTTT+GGG | + | chr8.4:28538011-28538030 | MS.gene28488:CDS | 25.0% |
| ! | TTTGACTATATAGATTCTCA+TGG | + | chr8.4:28538005-28538024 | MS.gene28488:CDS | 25.0% |
| !!! | ATAAATTTTGATACTCCTCT+TGG | - | chr8.4:28538343-28538362 | None:intergenic | 25.0% |
| !!! | CCATGTTGTGATTTTTTATA+AGG | - | chr8.4:28538407-28538426 | None:intergenic | 25.0% |
| !!! | TAAATTTTGATACTCCTCTT+GGG | - | chr8.4:28538342-28538361 | None:intergenic | 25.0% |
| !!! | TTACTTTTCTTTTTCTGACA+CGG | - | chr8.4:28537564-28537583 | None:intergenic | 25.0% |
| AACAAATGATCCACCATATT+GGG | + | chr8.4:28538675-28538694 | MS.gene28488:CDS | 30.0% | |
| AAGAAAAACAAGAGAACTGA+AGG | + | chr8.4:28536667-28536686 | MS.gene28488:CDS | 30.0% | |
| AAGAAGAAACTAATTGAGGA+AGG | + | chr8.4:28537501-28537520 | MS.gene28488:CDS | 30.0% | |
| AATGAATGTGAAGATGATGA+TGG | + | chr8.4:28536889-28536908 | MS.gene28488:CDS | 30.0% | |
| ATCATCATCTTCACATTCAT+TGG | - | chr8.4:28537463-28537482 | None:intergenic | 30.0% | |
| CATACTTTGTTAGAAAAGGA+GGG | + | chr8.4:28538152-28538171 | MS.gene28488:CDS | 30.0% | |
| CTATATAGATTCTCATGGTT+TGG | + | chr8.4:28538010-28538029 | MS.gene28488:CDS | 30.0% | |
| GAAAGAAGATGAAAAAGAAG+CGG | + | chr8.4:28536914-28536933 | MS.gene28488:CDS | 30.0% | |
| GTGTCAGAAAAAGAAAAGTA+AGG | + | chr8.4:28537563-28537582 | MS.gene28488:CDS | 30.0% | |
| GTTGCGAGATAATATTTGTT+GGG | + | chr8.4:28538430-28538449 | MS.gene28488:CDS | 30.0% | |
| TAAACGCAAACAAGTAACTT+GGG | + | chr8.4:28537890-28537909 | MS.gene28488:CDS | 30.0% | |
| TCAATAAATTCAAGCTCTTC+AGG | - | chr8.4:28538194-28538213 | None:intergenic | 30.0% | |
| TCATACTTTGTTAGAAAAGG+AGG | + | chr8.4:28538151-28538170 | MS.gene28488:CDS | 30.0% | |
| TCATCATCTTCACATTCATT+GGG | - | chr8.4:28536889-28536908 | None:intergenic | 30.0% | |
| TCTCTATCTTTCAACAAGTT+GGG | - | chr8.4:28538758-28538777 | None:intergenic | 30.0% | |
| TGAGCAATTAACATCAACAT+TGG | + | chr8.4:28538511-28538530 | MS.gene28488:CDS | 30.0% | |
| TGCTCATACTTTGTTAGAAA+AGG | + | chr8.4:28538148-28538167 | MS.gene28488:CDS | 30.0% | |
| TGTTGCGAGATAATATTTGT+TGG | + | chr8.4:28538429-28538448 | MS.gene28488:CDS | 30.0% | |
| TTTGATCTTCTCATCTTCTT+CGG | - | chr8.4:28537976-28537995 | None:intergenic | 30.0% | |
| ! | AAGAAGCACAAACTAATTGA+AGG | + | chr8.4:28537306-28537325 | MS.gene28488:CDS | 30.0% |
| ! | TCTTCTTCATTTTCTTTCCT+TGG | - | chr8.4:28537488-28537507 | None:intergenic | 30.0% |
| ! | TGTAATGACTTTAGAAGCAA+TGG | + | chr8.4:28537345-28537364 | MS.gene28488:CDS | 30.0% |
| !! | TCTTTTTCATCTTCTTTCCT+TGG | - | chr8.4:28536912-28536931 | None:intergenic | 30.0% |
| !! | TCTTTTTCGTCTTTTTTCCT+TGG | - | chr8.4:28537290-28537309 | None:intergenic | 30.0% |
| AAATGTCAAATTACACCCTG+AGG | + | chr8.4:28538058-28538077 | MS.gene28488:CDS | 35.0% | |
| AAGAAACTAATTGAGGAAGG+TGG | + | chr8.4:28537504-28537523 | MS.gene28488:CDS | 35.0% | |
| AATGAAACTCCTAGCAATGA+AGG | + | chr8.4:28537723-28537742 | MS.gene28488:CDS | 35.0% | |
| AATGAATGTGAAGATGACCA+AGG | + | chr8.4:28537270-28537289 | MS.gene28488:CDS | 35.0% | |
| AATTGAAGGAAACACACTGA+AGG | + | chr8.4:28537128-28537147 | MS.gene28488:CDS | 35.0% | |
| AATTGAAGGAAACACACTTG+AGG | + | chr8.4:28536942-28536961 | MS.gene28488:CDS | 35.0% | |
| ACAAATGATCCACCATATTG+GGG | + | chr8.4:28538676-28538695 | MS.gene28488:CDS | 35.0% | |
| ACATTGCTTTCGGACATATA+TGG | - | chr8.4:28536721-28536740 | None:intergenic | 35.0% | |
| AGAAACTAATTGAGGAAGGT+GGG | + | chr8.4:28537505-28537524 | MS.gene28488:CDS | 35.0% | |
| AGAAGCAATGAAGGTGATAA+TGG | + | chr8.4:28536979-28536998 | MS.gene28488:CDS | 35.0% | |
| CAGATCAGTTACATTGCTTT+CGG | - | chr8.4:28536731-28536750 | None:intergenic | 35.0% | |
| CCAAAGCAAAAAAAGGACAT+TGG | + | chr8.4:28538315-28538334 | MS.gene28488:CDS | 35.0% | |
| CTAAACGCAAACAAGTAACT+TGG | + | chr8.4:28537889-28537908 | MS.gene28488:CDS | 35.0% | |
| CTCTATCTTTCAACAAGTTG+GGG | - | chr8.4:28538757-28538776 | None:intergenic | 35.0% | |
| CTCTCTATCTTTCAACAAGT+TGG | - | chr8.4:28538759-28538778 | None:intergenic | 35.0% | |
| GAACAAATGATCCACCATAT+TGG | + | chr8.4:28538674-28538693 | MS.gene28488:CDS | 35.0% | |
| GATAGACGCTTTATATGCTT+TGG | + | chr8.4:28538574-28538593 | MS.gene28488:CDS | 35.0% | |
| GTCACTGAAGTTAATGATCA+AGG | + | chr8.4:28537636-28537655 | MS.gene28488:CDS | 35.0% | |
| GTCACTGATGTTAATGATCA+AGG | + | chr8.4:28537768-28537787 | MS.gene28488:CDS | 35.0% | |
| GTTTCATTATGTGCACAGTT+AGG | - | chr8.4:28537711-28537730 | None:intergenic | 35.0% | |
| TTATGTGTGCTTTCTTCACT+TGG | - | chr8.4:28537228-28537247 | None:intergenic | 35.0% | |
| TTGATCTTCTCATCTTCTTC+GGG | - | chr8.4:28537975-28537994 | None:intergenic | 35.0% | |
| ! | AAGTGAAGAAAGCACACATA+AGG | + | chr8.4:28537227-28537246 | MS.gene28488:CDS | 35.0% |
| ! | AATATTTGTTGGGAGGCAAT+TGG | + | chr8.4:28538440-28538459 | MS.gene28488:CDS | 35.0% |
| ! | AATGACTTTAGAAGCAATGG+AGG | + | chr8.4:28537348-28537367 | MS.gene28488:CDS | 35.0% |
| ! | AGAAGCAATGATGATGAAGA+TGG | + | chr8.4:28537165-28537184 | MS.gene28488:CDS | 35.0% |
| ! | CATATAAAGCGTCTATCAAG+CGG | - | chr8.4:28538571-28538590 | None:intergenic | 35.0% |
| ! | TCACCATTCCTTTTGATCAA+AGG | - | chr8.4:28536754-28536773 | None:intergenic | 35.0% |
| !! | AATCACAGGTTTGTTGTCAA+AGG | - | chr8.4:28538783-28538802 | None:intergenic | 35.0% |
| !! | CAACTTGTTGAAAGATAGAG+AGG | + | chr8.4:28538757-28538776 | MS.gene28488:CDS | 35.0% |
| !!! | CCAATGTCCTTTTTTTGCTT+TGG | - | chr8.4:28538318-28538337 | None:intergenic | 35.0% |
| !!! | GTTCTCTTGTTTTTCTTGCT+CGG | - | chr8.4:28536664-28536683 | None:intergenic | 35.0% |
| AAACCTAGATCTGCTTGAGA+GGG | + | chr8.4:28538373-28538392 | MS.gene28488:CDS | 40.0% | |
| AAATTACACCCTGAGGTTAG+CGG | + | chr8.4:28538065-28538084 | MS.gene28488:CDS | 40.0% | |
| AACCTGCTCAATGAATGACT+TGG | - | chr8.4:28538711-28538730 | None:intergenic | 40.0% | |
| AAGAAGCGGAAGCTAATTGA+AGG | + | chr8.4:28536928-28536947 | MS.gene28488:CDS | 40.0% | |
| AATCTACTTGAGCATAGGTC+TGG | + | chr8.4:28538629-28538648 | MS.gene28488:CDS | 40.0% | |
| ACTCCTAACTCTGCACATAA+TGG | + | chr8.4:28537861-28537880 | MS.gene28488:CDS | 40.0% | |
| CTGATCTGCCTTTGATCAAA+AGG | + | chr8.4:28536743-28536762 | MS.gene28488:CDS | 40.0% | |
| CTGCCTTTGATCAAAAGGAA+TGG | + | chr8.4:28536748-28536767 | MS.gene28488:CDS | 40.0% | |
| CTTTGTTAGAAAAGGAGGGA+AGG | + | chr8.4:28538156-28538175 | MS.gene28488:CDS | 40.0% | |
| GAAAAGACCTATGAGCAACA+TGG | + | chr8.4:28538212-28538231 | MS.gene28488:CDS | 40.0% | |
| GAATGTGAAGATGATGACCA+AGG | + | chr8.4:28536892-28536911 | MS.gene28488:CDS | 40.0% | |
| GAGGAGAAGTTATGTTGTGA+CGG | + | chr8.4:28537924-28537943 | MS.gene28488:CDS | 40.0% | |
| GAGTGACGAAAGCAAACATA+AGG | + | chr8.4:28537038-28537057 | MS.gene28488:CDS | 40.0% | |
| GTTCCATTATGTGCAGAGTT+AGG | - | chr8.4:28537867-28537886 | None:intergenic | 40.0% | |
| TAAACCTAGATCTGCTTGAG+AGG | + | chr8.4:28538372-28538391 | MS.gene28488:CDS | 40.0% | |
| TATTCTTCTTCGTCTTGCCT+TGG | - | chr8.4:28537539-28537558 | None:intergenic | 40.0% | |
| ! | ACATCAACATTGGTTGCTAG+TGG | + | chr8.4:28538521-28538540 | MS.gene28488:CDS | 40.0% |
| ! | GCGAGATAATATTTGTTGGG+AGG | + | chr8.4:28538433-28538452 | MS.gene28488:CDS | 40.0% |
| ! | TTGCAAAAAGCATACCTGAG+AGG | + | chr8.4:28538102-28538121 | MS.gene28488:CDS | 40.0% |
| AAAAGGACATTGGTCCCAAG+AGG | + | chr8.4:28538325-28538344 | MS.gene28488:CDS | 45.0% | |
| AAAAGTGAAGATGGCGAGCA+AGG | + | chr8.4:28536796-28536815 | MS.gene28488:CDS | 45.0% | |
| AACACACTTGAGGAAGGTGA+CGG | + | chr8.4:28536952-28536971 | MS.gene28488:CDS | 45.0% | |
| AAGAGAACTGAAGGCGAAGA+GGG | + | chr8.4:28536676-28536695 | MS.gene28488:CDS | 45.0% | |
| ACTAGCTCCATGTTGCTCAT+AGG | - | chr8.4:28538222-28538241 | None:intergenic | 45.0% | |
| AGAAGCAATGGAGGTGAAGA+GGG | + | chr8.4:28537357-28537376 | MS.gene28488:CDS | 45.0% | |
| AGTAACTTGGGCTGATGAAG+TGG | + | chr8.4:28537902-28537921 | MS.gene28488:CDS | 45.0% | |
| ATGAGCAACATGGAGCTAGT+TGG | + | chr8.4:28538222-28538241 | MS.gene28488:CDS | 45.0% | |
| CATATATGGATCGCCTCCAT+TGG | - | chr8.4:28536707-28536726 | None:intergenic | 45.0% | |
| CGTCGATACACACTCTCATA+AGG | - | chr8.4:28538128-28538147 | None:intergenic | 45.0% | |
| GAAGGAAACACACTTGAGGA+AGG | + | chr8.4:28536946-28536965 | MS.gene28488:CDS | 45.0% | |
| GAATGTGAAGCTGATGACCA+AGG | + | chr8.4:28537084-28537103 | MS.gene28488:CDS | 45.0% | |
| GGGACAATCTACTTGAGCAT+AGG | + | chr8.4:28538624-28538643 | MS.gene28488:CDS | 45.0% | |
| TAGAAGCAATGGAGGTGAAG+AGG | + | chr8.4:28537356-28537375 | MS.gene28488:CDS | 45.0% | |
| TCAGCATCACCTTCATTGCT+AGG | - | chr8.4:28537735-28537754 | None:intergenic | 45.0% | |
| TGATGTGTGCAGGAAACGAT+GGG | + | chr8.4:28538652-28538671 | MS.gene28488:CDS | 45.0% | |
| TTTGGGTAAAAGTCGAGCTC+AGG | + | chr8.4:28538262-28538281 | MS.gene28488:CDS | 45.0% | |
| ! | AAAAGGAGGGAAGGTGTAAG+TGG | + | chr8.4:28538165-28538184 | MS.gene28488:CDS | 45.0% |
| ! | CATTGGTTGCTAGTGGTGAT+TGG | + | chr8.4:28538528-28538547 | MS.gene28488:CDS | 45.0% |
| ! | CTGCTCAATGAATGACTTGG+CGG | - | chr8.4:28538708-28538727 | None:intergenic | 45.0% |
| ! | GTGCATTTTGCTGCAAGAAG+TGG | + | chr8.4:28538486-28538505 | MS.gene28488:CDS | 45.0% |
| !! | GACGGCTTTAGAAGCAATGA+AGG | + | chr8.4:28536970-28536989 | MS.gene28488:CDS | 45.0% |
| !! | TGTGAAGATGATGATGGCCA+AGG | + | chr8.4:28537468-28537487 | MS.gene28488:CDS | 45.0% |
| AACTTGGGCTGATGAAGTGG+AGG | + | chr8.4:28537905-28537924 | MS.gene28488:CDS | 50.0% | |
| ACACTCTCATAAGGCCTCTC+AGG | - | chr8.4:28538119-28538138 | None:intergenic | 50.0% | |
| ACGGCTTATTGCGTGGGAAA+AGG | + | chr8.4:28537943-28537962 | MS.gene28488:CDS | 50.0% | |
| AGAGCAGTAGCTGACGCTTT+GGG | + | chr8.4:28538245-28538264 | MS.gene28488:CDS | 50.0% | |
| CAAGAGAACTGAAGGCGAAG+AGG | + | chr8.4:28536675-28536694 | MS.gene28488:CDS | 50.0% | |
| CGCCAAGTCATTCATTGAGC+AGG | + | chr8.4:28538706-28538725 | MS.gene28488:CDS | 50.0% | |
| GAAGAGGGTGAAACCAATGG+AGG | + | chr8.4:28536691-28536710 | MS.gene28488:CDS | 50.0% | |
| GAGCTCAGGTAAAAGATGCG+TGG | + | chr8.4:28538276-28538295 | MS.gene28488:CDS | 50.0% | |
| GGAGGCAATTGGTCACAAGT+TGG | + | chr8.4:28538451-28538470 | MS.gene28488:CDS | 50.0% | |
| GGGAGACGAAGGTCTTGATA+TGG | + | chr8.4:28538031-28538050 | MS.gene28488:CDS | 50.0% | |
| GTGATGTGTGCAGGAAACGA+TGG | + | chr8.4:28538651-28538670 | MS.gene28488:CDS | 50.0% | |
| GTTGTGACGGCTTATTGCGT+GGG | + | chr8.4:28537937-28537956 | MS.gene28488:CDS | 50.0% | |
| TAGCCCTCTCAAGCAGATCT+AGG | - | chr8.4:28538379-28538398 | None:intergenic | 50.0% | |
| TGTTGTGACGGCTTATTGCG+TGG | + | chr8.4:28537936-28537955 | MS.gene28488:CDS | 50.0% | |
| ! | ATAGGTCTGGTGATGTGTGC+AGG | + | chr8.4:28538642-28538661 | MS.gene28488:CDS | 50.0% |
| ! | TCTCATGGTTTGGGAGACGA+AGG | + | chr8.4:28538020-28538039 | MS.gene28488:CDS | 50.0% |
| ! | TGCTTTGGATGCTTGCTGCA+TGG | + | chr8.4:28538589-28538608 | MS.gene28488:CDS | 50.0% |
| ! | TTTGGATGCTTGCTGCATGG+AGG | + | chr8.4:28538592-28538611 | MS.gene28488:CDS | 50.0% |
| !! | GAAGGTGATAATGGCGAGCA+GGG | + | chr8.4:28536988-28537007 | MS.gene28488:CDS | 50.0% |
| !! | TGAAGGTGATAATGGCGAGC+AGG | + | chr8.4:28536987-28537006 | MS.gene28488:CDS | 50.0% |
| CTGCATGGAGGAGGTAGACT+GGG | + | chr8.4:28538604-28538623 | MS.gene28488:CDS | 55.0% | |
| GAAGGTGGGAATCGTGACCA+AGG | + | chr8.4:28537519-28537538 | MS.gene28488:CDS | 55.0% | |
| GAGAGCAGTAGCTGACGCTT+TGG | + | chr8.4:28538244-28538263 | MS.gene28488:CDS | 55.0% | |
| GGCGAAGAGGGTGAAACCAA+TGG | + | chr8.4:28536688-28536707 | MS.gene28488:CDS | 55.0% | |
| TCCAACAGCCGCTAACCTCA+GGG | - | chr8.4:28538076-28538095 | None:intergenic | 55.0% | |
| TTCCAACAGCCGCTAACCTC+AGG | - | chr8.4:28538077-28538096 | None:intergenic | 55.0% | |
| ACCCTGAGGTTAGCGGCTGT+TGG | + | chr8.4:28538072-28538091 | MS.gene28488:CDS | 60.0% | |
| CGGCATGCTCCCCAATATGG+TGG | - | chr8.4:28538688-28538707 | None:intergenic | 60.0% | |
| GCTGCATGGAGGAGGTAGAC+TGG | + | chr8.4:28538603-28538622 | MS.gene28488:CDS | 60.0% | |
| TGGCGGCATGCTCCCCAATA+TGG | - | chr8.4:28538691-28538710 | None:intergenic | 60.0% | |
| ! | GGATGCTTGCTGCATGGAGG+AGG | + | chr8.4:28538595-28538614 | MS.gene28488:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 28536652 | 28538808 | 28536652 | ID=MS.gene28488 |
| chr8.4 | mRNA | 28536652 | 28538808 | 28536652 | ID=MS.gene28488.t1;Parent=MS.gene28488 |
| chr8.4 | exon | 28536652 | 28538808 | 28536652 | ID=MS.gene28488.t1.exon1;Parent=MS.gene28488.t1 |
| chr8.4 | CDS | 28536652 | 28538808 | 28536652 | ID=cds.MS.gene28488.t1;Parent=MS.gene28488.t1 |
| Gene Sequence |
| Protein sequence |
