Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31862.t1 | XP_013466443.1 | 91.4 | 222 | 17 | 1 | 1 | 220 | 1 | 222 | 4.90E-103 | 384 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31862.t1 | A0A072VEP6 | 91.4 | 222 | 17 | 1 | 1 | 220 | 1 | 222 | 3.5e-103 | 384.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene31862 | 0.808989 | 2.36E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene31862 | 0.82428 | 8.79E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene31862 | 0.80644 | 8.22E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene31862 | -0.83458 | 2.76E-56 | -1.69E-46 |
| MS.gene049499 | MS.gene31862 | 0.834547 | 2.81E-56 | -1.69E-46 |
| MS.gene049786 | MS.gene31862 | 0.80789 | 4.05E-50 | -1.69E-46 |
| MS.gene049801 | MS.gene31862 | 0.8053 | 1.43E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene31862 | 0.834848 | 2.36E-56 | -1.69E-46 |
| MS.gene050317 | MS.gene31862 | 0.800488 | 1.41E-48 | -1.69E-46 |
| MS.gene050646 | MS.gene31862 | 0.82149 | 3.93E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene31862 | 0.839003 | 2.05E-57 | -1.69E-46 |
| MS.gene050737 | MS.gene31862 | 0.801809 | 7.56E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene31862 | 0.835798 | 1.36E-56 | -1.69E-46 |
| MS.gene051003 | MS.gene31862 | 0.813047 | 3.12E-51 | -1.69E-46 |
| MS.gene051452 | MS.gene31862 | 0.809628 | 1.72E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene31862 | 0.812489 | 4.13E-51 | -1.69E-46 |
| MS.gene051856 | MS.gene31862 | 0.835123 | 2.02E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene31862 | 0.811002 | 8.71E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene31862 | 0.800751 | 1.24E-48 | -1.69E-46 |
| MS.gene052434 | MS.gene31862 | 0.81547 | 9.10E-52 | -1.69E-46 |
| MS.gene052518 | MS.gene31862 | 0.811641 | 6.32E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene31862 | 0.80402 | 2.64E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene31862 | 0.80884 | 2.54E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene31862 | 0.813707 | 2.23E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene31862 | 0.827928 | 1.19E-54 | -1.69E-46 |
| MS.gene05517 | MS.gene31862 | 0.814615 | 1.41E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene31862 | 0.803019 | 4.26E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene31862 | 0.802275 | 6.06E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene31862 | 0.801165 | 1.02E-48 | -1.69E-46 |
| MS.gene056045 | MS.gene31862 | 0.810022 | 1.42E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene31862 | 0.850304 | 1.85E-60 | -1.69E-46 |
| MS.gene056787 | MS.gene31862 | 0.827617 | 1.42E-54 | -1.69E-46 |
| MS.gene056831 | MS.gene31862 | 0.808543 | 2.94E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene31862 | 0.80939 | 1.94E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene31862 | 0.83463 | 2.68E-56 | -1.69E-46 |
| MS.gene057890 | MS.gene31862 | -0.817181 | 3.77E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene31862 | 0.803439 | 3.48E-49 | -1.69E-46 |
| MS.gene058708 | MS.gene31862 | -0.813908 | 2.02E-51 | -1.69E-46 |
| MS.gene058836 | MS.gene31862 | 0.826735 | 2.30E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene31862 | 0.815955 | 7.09E-52 | -1.69E-46 |
| MS.gene059360 | MS.gene31862 | 0.822478 | 2.32E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene31862 | 0.83359 | 4.89E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene31862 | 0.805247 | 1.46E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene31862 | 0.802599 | 5.20E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene31862 | 0.811672 | 6.23E-51 | -1.69E-46 |
| MS.gene060777 | MS.gene31862 | 0.80467 | 1.93E-49 | -1.69E-46 |
| MS.gene060851 | MS.gene31862 | 0.80412 | 2.52E-49 | -1.69E-46 |
| MS.gene060852 | MS.gene31862 | 0.805525 | 1.28E-49 | -1.69E-46 |
| MS.gene060870 | MS.gene31862 | 0.818905 | 1.54E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene31862 | 0.815304 | 9.90E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene31862 | 0.807616 | 4.63E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene31862 | 0.829248 | 5.72E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene31862 | 0.833579 | 4.92E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene31862 | 0.8011 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31862.t1 | MTR_1g029550 | 96.396 | 222 | 6 | 1 | 1 | 220 | 1 | 222 | 9.35e-159 | 440 |
| MS.gene31862.t1 | MTR_3g088815 | 39.524 | 210 | 96 | 7 | 1 | 196 | 1 | 193 | 5.93e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31862.t1 | AT1G76980 | 39.899 | 198 | 96 | 8 | 22 | 201 | 6 | 198 | 9.29e-29 | 109 |
| MS.gene31862.t1 | AT1G76980 | 39.899 | 198 | 96 | 8 | 22 | 201 | 6 | 198 | 1.87e-28 | 108 |
| MS.gene31862.t1 | AT1G21390 | 41.765 | 170 | 79 | 5 | 44 | 196 | 26 | 192 | 6.33e-27 | 104 |
Find 44 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATGTGTTCTCCACCTTTA+TGG | 0.164256 | 1.4:+14588273 | MS.gene31862:CDS |
| TTAGATGATCCCAAAGAAAT+TGG | 0.202956 | 1.4:-14588587 | None:intergenic |
| TGATAAGAAGAAAGAGTTAT+TGG | 0.226827 | 1.4:+14588616 | MS.gene31862:CDS |
| AAGAGGTCAAAGAGAGTTAA+TGG | 0.266398 | 1.4:+14588361 | MS.gene31862:CDS |
| ATGGCTTGTGTCTTTGATTC+AGG | 0.274722 | 1.4:-14588335 | None:intergenic |
| GTAAGTTCATAGCTTGATTC+TGG | 0.277190 | 1.4:-14588401 | None:intergenic |
| TTACTCTTTGTTGTCCATAA+AGG | 0.348229 | 1.4:-14588287 | None:intergenic |
| TGTATTCTATCATCAGAAAC+TGG | 0.389778 | 1.4:-14588182 | None:intergenic |
| CTCTCTTTGACCTCTTTCAA+TGG | 0.402619 | 1.4:-14588354 | None:intergenic |
| TTCTGATGGATCTGTTAGTA+AGG | 0.426899 | 1.4:+14588688 | MS.gene31862:CDS |
| GGATTGGTGGAAGAAGAAAC+TGG | 0.442020 | 1.4:+14588709 | MS.gene31862:CDS |
| TCAAAGAGAGTTAATGGAAA+TGG | 0.447636 | 1.4:+14588367 | MS.gene31862:CDS |
| CAACATGTTGAAGATGAATC+TGG | 0.475461 | 1.4:+14588251 | MS.gene31862:CDS |
| AAGAAATTGTGGGTTGGTAA+TGG | 0.479830 | 1.4:-14588157 | None:intergenic |
| CTCTTTGTTGTCCATAAAGG+TGG | 0.488907 | 1.4:-14588284 | None:intergenic |
| CCAAGAGCATCTGTTTCTGA+TGG | 0.489074 | 1.4:+14588674 | MS.gene31862:CDS |
| AGAAACTGGAAAAGAAATTG+TGG | 0.502575 | 1.4:-14588168 | None:intergenic |
| GATCTGTTAGTAAGGATTGG+TGG | 0.520890 | 1.4:+14588696 | MS.gene31862:CDS |
| TAAAAGTAGTAGTAATTCAA+AGG | 0.523482 | 1.4:+14588646 | MS.gene31862:CDS |
| AAAGTGAAACATAACTAGCC+GGG | 0.523707 | 1.4:-14589113 | None:intergenic |
| GAAACTGGAAAAGAAATTGT+GGG | 0.525639 | 1.4:-14588167 | None:intergenic |
| AAGACACAAGCCATTGAAAG+AGG | 0.538043 | 1.4:+14588344 | MS.gene31862:CDS |
| AAAATAACAAGGTAAAAGAG+AGG | 0.540508 | 1.4:+14588459 | MS.gene31862:CDS |
| CCATCAGAAACAGATGCTCT+TGG | 0.545084 | 1.4:-14588674 | None:intergenic |
| CAAAGTGAAACATAACTAGC+CGG | 0.548125 | 1.4:-14589114 | None:intergenic |
| GGTAGTAGTGGTTCAGAATG+TGG | 0.553707 | 1.4:+14588731 | MS.gene31862:CDS |
| AATTAGGGCTAGTAGAAATG+TGG | 0.563530 | 1.4:+14588517 | MS.gene31862:CDS |
| CTGGAAAAGAAATTGTGGGT+TGG | 0.579799 | 1.4:-14588163 | None:intergenic |
| AGAAGAATATTGACAGTGGA+GGG | 0.588979 | 1.4:+14588543 | MS.gene31862:CDS |
| ATGGATCTGTTAGTAAGGAT+TGG | 0.591287 | 1.4:+14588693 | MS.gene31862:CDS |
| GGTTGGTAATGGTATCTTCG+TGG | 0.594866 | 1.4:-14588146 | None:intergenic |
| AAGTGAAACATAACTAGCCG+GGG | 0.603226 | 1.4:-14589112 | None:intergenic |
| ATTGACTCGTATTCTAGCGA+AGG | 0.610173 | 1.4:+14589085 | MS.gene31862:CDS |
| AAGAAGAATATTGACAGTGG+AGG | 0.610321 | 1.4:+14588542 | MS.gene31862:CDS |
| AAGAAGAAACTGGGTAGTAG+TGG | 0.612108 | 1.4:+14588719 | MS.gene31862:CDS |
| GATAAGAAGAATATTGACAG+TGG | 0.625867 | 1.4:+14588539 | MS.gene31862:CDS |
| GATTGGTGGAAGAAGAAACT+GGG | 0.633283 | 1.4:+14588710 | MS.gene31862:CDS |
| TACTTTGAAAGATCTTGTTG+AGG | 0.634719 | 1.4:+14588421 | MS.gene31862:CDS |
| AGTGGTGTATCTAGCATGAG+TGG | 0.636022 | 1.4:+14588773 | MS.gene31862:CDS |
| ATTCTAGCGAAGGAGACCCC+CGG | 0.676484 | 1.4:+14589095 | MS.gene31862:CDS |
| AGTAGTGGTTCAGAATGTGG+AGG | 0.678513 | 1.4:+14588734 | MS.gene31862:CDS |
| TCATGTTGAAGAAAATAACA+AGG | 0.690119 | 1.4:+14588448 | MS.gene31862:CDS |
| GGTGATCAAAGTGATCATAG+TGG | 0.721338 | 1.4:+14588755 | MS.gene31862:CDS |
| AGTGAAACATAACTAGCCGG+GGG | 0.725184 | 1.4:-14589111 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGAAAGGAAAAAAAAAAA+AGG | - | chr1.4:14589031-14589050 | None:intergenic | 15.0% |
| !! | TAAAAGTAGTAGTAATTCAA+AGG | + | chr1.4:14588646-14588665 | MS.gene31862:CDS | 20.0% |
| !!! | AATGATTTTTCCAATTTCTT+TGG | + | chr1.4:14588577-14588596 | MS.gene31862:CDS | 20.0% |
| !!! | ATGATTTTTCCAATTTCTTT+GGG | + | chr1.4:14588578-14588597 | MS.gene31862:CDS | 20.0% |
| !!! | CTATTGTAAGTTTTAGATTT+TGG | + | chr1.4:14588974-14588993 | MS.gene31862:intron | 20.0% |
| !!! | TTTTTTTCCTTTCAAACTAT+GGG | + | chr1.4:14589035-14589054 | MS.gene31862:intron | 20.0% |
| !!! | TTTTTTTTCCTTTCAAACTA+TGG | + | chr1.4:14589034-14589053 | MS.gene31862:intron | 20.0% |
| ! | AAAATAACAAGGTAAAAGAG+AGG | + | chr1.4:14588459-14588478 | MS.gene31862:CDS | 25.0% |
| ! | TCATGTTGAAGAAAATAACA+AGG | + | chr1.4:14588448-14588467 | MS.gene31862:CDS | 25.0% |
| ! | TGATAAGAAGAAAGAGTTAT+TGG | + | chr1.4:14588616-14588635 | MS.gene31862:CDS | 25.0% |
| AAAAACACACGAAAAAGAGA+AGG | + | chr1.4:14588494-14588513 | MS.gene31862:CDS | 30.0% | |
| AACAAATCCCATAGTTTGAA+AGG | - | chr1.4:14589045-14589064 | None:intergenic | 30.0% | |
| ACGAAAAAGAGAAGGAATTA+GGG | + | chr1.4:14588502-14588521 | MS.gene31862:CDS | 30.0% | |
| AGAAACTGGAAAAGAAATTG+TGG | - | chr1.4:14588171-14588190 | None:intergenic | 30.0% | |
| GAAACTGGAAAAGAAATTGT+GGG | - | chr1.4:14588170-14588189 | None:intergenic | 30.0% | |
| GATAAGAAGAATATTGACAG+TGG | + | chr1.4:14588539-14588558 | MS.gene31862:CDS | 30.0% | |
| TCAAAGAGAGTTAATGGAAA+TGG | + | chr1.4:14588367-14588386 | MS.gene31862:CDS | 30.0% | |
| TGTATTCTATCATCAGAAAC+TGG | - | chr1.4:14588185-14588204 | None:intergenic | 30.0% | |
| TTAGATGATCCCAAAGAAAT+TGG | - | chr1.4:14588590-14588609 | None:intergenic | 30.0% | |
| ! | TACTTTGAAAGATCTTGTTG+AGG | + | chr1.4:14588421-14588440 | MS.gene31862:CDS | 30.0% |
| ! | TTACTCTTTGTTGTCCATAA+AGG | - | chr1.4:14588290-14588309 | None:intergenic | 30.0% |
| !!! | GTAAGTTTTAGATTTTGGCA+TGG | + | chr1.4:14588979-14588998 | MS.gene31862:intron | 30.0% |
| AAAAAAGGATTGCTCCTTGT+AGG | - | chr1.4:14589016-14589035 | None:intergenic | 35.0% | |
| AAGAAGAATATTGACAGTGG+AGG | + | chr1.4:14588542-14588561 | MS.gene31862:CDS | 35.0% | |
| AAGAGGTCAAAGAGAGTTAA+TGG | + | chr1.4:14588361-14588380 | MS.gene31862:CDS | 35.0% | |
| AGAAGAATATTGACAGTGGA+GGG | + | chr1.4:14588543-14588562 | MS.gene31862:CDS | 35.0% | |
| AGTGGTAGCATCAAAAGATA+TGG | + | chr1.4:14588791-14588810 | MS.gene31862:intron | 35.0% | |
| ATGGATCTGTTAGTAAGGAT+TGG | + | chr1.4:14588693-14588712 | MS.gene31862:CDS | 35.0% | |
| CAACATGTTGAAGATGAATC+TGG | + | chr1.4:14588251-14588270 | MS.gene31862:CDS | 35.0% | |
| CACGAAAAAGAGAAGGAATT+AGG | + | chr1.4:14588501-14588520 | MS.gene31862:CDS | 35.0% | |
| GTAAGTTCATAGCTTGATTC+TGG | - | chr1.4:14588404-14588423 | None:intergenic | 35.0% | |
| TGGTAATTAACAAGCCTACA+AGG | + | chr1.4:14588999-14589018 | MS.gene31862:intron | 35.0% | |
| ! | AATTAGGGCTAGTAGAAATG+TGG | + | chr1.4:14588517-14588536 | MS.gene31862:CDS | 35.0% |
| ! | ACTATGGGATTTGTTTCGTT+AGG | + | chr1.4:14589050-14589069 | MS.gene31862:intron | 35.0% |
| ! | TTCTGATGGATCTGTTAGTA+AGG | + | chr1.4:14588688-14588707 | MS.gene31862:CDS | 35.0% |
| !! | AAGAAATTGTGGGTTGGTAA+TGG | - | chr1.4:14588160-14588179 | None:intergenic | 35.0% |
| AAGAAGAAACTGGGTAGTAG+TGG | + | chr1.4:14588719-14588738 | MS.gene31862:CDS | 40.0% | |
| AAGACACAAGCCATTGAAAG+AGG | + | chr1.4:14588344-14588363 | MS.gene31862:CDS | 40.0% | |
| CTCTCTTTGACCTCTTTCAA+TGG | - | chr1.4:14588357-14588376 | None:intergenic | 40.0% | |
| CTGGAAAAGAAATTGTGGGT+TGG | - | chr1.4:14588166-14588185 | None:intergenic | 40.0% | |
| GATCTGTTAGTAAGGATTGG+TGG | + | chr1.4:14588696-14588715 | MS.gene31862:CDS | 40.0% | |
| GATTGGTGGAAGAAGAAACT+GGG | + | chr1.4:14588710-14588729 | MS.gene31862:CDS | 40.0% | |
| GGTGATCAAAGTGATCATAG+TGG | + | chr1.4:14588755-14588774 | MS.gene31862:CDS | 40.0% | |
| GTATGTGTTCTCCACCTTTA+TGG | + | chr1.4:14588273-14588292 | MS.gene31862:CDS | 40.0% | |
| ! | ATGGCTTGTGTCTTTGATTC+AGG | - | chr1.4:14588338-14588357 | None:intergenic | 40.0% |
| ! | CTCTTTGTTGTCCATAAAGG+TGG | - | chr1.4:14588287-14588306 | None:intergenic | 40.0% |
| !! | ATTGACTCGTATTCTAGCGA+AGG | + | chr1.4:14589085-14589104 | MS.gene31862:CDS | 40.0% |
| AGTAGTGGTTCAGAATGTGG+AGG | + | chr1.4:14588734-14588753 | MS.gene31862:CDS | 45.0% | |
| AGTGGTGTATCTAGCATGAG+TGG | + | chr1.4:14588773-14588792 | MS.gene31862:CDS | 45.0% | |
| CCATCAGAAACAGATGCTCT+TGG | - | chr1.4:14588677-14588696 | None:intergenic | 45.0% | |
| GGTAGTAGTGGTTCAGAATG+TGG | + | chr1.4:14588731-14588750 | MS.gene31862:CDS | 45.0% | |
| ! | GGATTGGTGGAAGAAGAAAC+TGG | + | chr1.4:14588709-14588728 | MS.gene31862:CDS | 45.0% |
| !! | CCAAGAGCATCTGTTTCTGA+TGG | + | chr1.4:14588674-14588693 | MS.gene31862:CDS | 45.0% |
| !! | GGTTGGTAATGGTATCTTCG+TGG | - | chr1.4:14588149-14588168 | None:intergenic | 45.0% |
| !! | AAAAATTACTTATAATTATA+TGG | - | chr1.4:14588875-14588894 | None:intergenic | 5.0% |
| ACTCCTGCTGCTACAACTAC+TGG | - | chr1.4:14588828-14588847 | None:intergenic | 50.0% | |
| GAACCAGTAGTTGTAGCAGC+AGG | + | chr1.4:14588822-14588841 | MS.gene31862:intron | 50.0% | |
| GTAGCAGCAGGAGTAATAGC+AGG | + | chr1.4:14588834-14588853 | MS.gene31862:intron | 50.0% | |
| ATTCTAGCGAAGGAGACCCC+CGG | + | chr1.4:14589095-14589114 | MS.gene31862:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 14588137 | 14589121 | 14588137 | ID=MS.gene31862 |
| chr1.4 | mRNA | 14588137 | 14589121 | 14588137 | ID=MS.gene31862.t1;Parent=MS.gene31862 |
| chr1.4 | exon | 14588137 | 14588794 | 14588137 | ID=MS.gene31862.t1.exon1;Parent=MS.gene31862.t1 |
| chr1.4 | CDS | 14588137 | 14588794 | 14588137 | ID=cds.MS.gene31862.t1;Parent=MS.gene31862.t1 |
| chr1.4 | exon | 14589072 | 14589121 | 14589072 | ID=MS.gene31862.t1.exon2;Parent=MS.gene31862.t1 |
| chr1.4 | CDS | 14589072 | 14589121 | 14589072 | ID=cds.MS.gene31862.t1;Parent=MS.gene31862.t1 |
| Gene Sequence |
| Protein sequence |
