Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34687.t1 | XP_003628363.1 | 97.9 | 336 | 7 | 0 | 1 | 336 | 1 | 336 | 1.00E-179 | 639.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34687.t1 | Q6AY79 | 31.5 | 343 | 206 | 13 | 3 | 328 | 25 | 355 | 4.7e-24 | 113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34687.t1 | G7LA57 | 97.9 | 336 | 7 | 0 | 1 | 336 | 1 | 336 | 7.4e-180 | 639.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene34687 | 0.823615 | 1.26E-53 | -1.69E-46 |
| MS.gene049255 | MS.gene34687 | 0.814307 | 1.65E-51 | -1.69E-46 |
| MS.gene049382 | MS.gene34687 | 0.817721 | 2.85E-52 | -1.69E-46 |
| MS.gene049424 | MS.gene34687 | 0.806503 | 7.97E-50 | -1.69E-46 |
| MS.gene049756 | MS.gene34687 | 0.802501 | 5.45E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene34687 | 0.803576 | 3.26E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene34687 | 0.800392 | 1.47E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene34687 | 0.839336 | 1.68E-57 | -1.69E-46 |
| MS.gene050303 | MS.gene34687 | 0.824734 | 6.88E-54 | -1.69E-46 |
| MS.gene050605 | MS.gene34687 | 0.800609 | 1.33E-48 | -1.69E-46 |
| MS.gene050623 | MS.gene34687 | -0.800867 | 1.18E-48 | -1.69E-46 |
| MS.gene050721 | MS.gene34687 | 0.800823 | 1.20E-48 | -1.69E-46 |
| MS.gene050724 | MS.gene34687 | 0.802212 | 6.24E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene34687 | 0.80947 | 1.86E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene34687 | 0.818082 | 2.36E-52 | -1.69E-46 |
| MS.gene050987 | MS.gene34687 | 0.809584 | 1.76E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene34687 | 0.826753 | 2.28E-54 | -1.69E-46 |
| MS.gene051245 | MS.gene34687 | 0.80247 | 5.53E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene34687 | 0.819044 | 1.43E-52 | -1.69E-46 |
| MS.gene05134 | MS.gene34687 | 0.836279 | 1.03E-56 | -1.69E-46 |
| MS.gene051535 | MS.gene34687 | 0.806282 | 8.87E-50 | -1.69E-46 |
| MS.gene051757 | MS.gene34687 | 0.805203 | 1.50E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene34687 | 0.811181 | 7.96E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene34687 | 0.842 | 3.38E-58 | -1.69E-46 |
| MS.gene052302 | MS.gene34687 | 0.827642 | 1.40E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene34687 | 0.835433 | 1.68E-56 | -1.69E-46 |
| MS.gene052426 | MS.gene34687 | 0.824309 | 8.65E-54 | -1.69E-46 |
| MS.gene052856 | MS.gene34687 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene34687 | 0.800916 | 1.15E-48 | -1.69E-46 |
| MS.gene053216 | MS.gene34687 | 0.811522 | 6.71E-51 | -1.69E-46 |
| MS.gene053381 | MS.gene34687 | 0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene054657 | MS.gene34687 | 0.806604 | 7.59E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene34687 | 0.816142 | 6.44E-52 | -1.69E-46 |
| MS.gene055162 | MS.gene34687 | 0.807463 | 4.99E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene34687 | 0.810546 | 1.09E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene34687 | 0.808941 | 2.42E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene34687 | 0.805697 | 1.18E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene34687 | 0.823422 | 1.40E-53 | -1.69E-46 |
| MS.gene056760 | MS.gene34687 | 0.831941 | 1.25E-55 | -1.69E-46 |
| MS.gene057140 | MS.gene34687 | 0.836209 | 1.07E-56 | -1.69E-46 |
| MS.gene057234 | MS.gene34687 | 0.813118 | 3.01E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene34687 | 0.826931 | 2.07E-54 | -1.69E-46 |
| MS.gene05795 | MS.gene34687 | 0.81816 | 2.27E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene34687 | 0.800998 | 1.11E-48 | -1.69E-46 |
| MS.gene058238 | MS.gene34687 | 0.823219 | 1.56E-53 | -1.69E-46 |
| MS.gene058399 | MS.gene34687 | 0.811083 | 8.36E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene34687 | 0.813652 | 2.30E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene34687 | 0.831517 | 1.60E-55 | -1.69E-46 |
| MS.gene059249 | MS.gene34687 | 0.818249 | 2.16E-52 | -1.69E-46 |
| MS.gene059317 | MS.gene34687 | 0.849927 | 2.36E-60 | -1.69E-46 |
| MS.gene05979 | MS.gene34687 | 0.800547 | 1.37E-48 | -1.69E-46 |
| MS.gene059830 | MS.gene34687 | 0.81359 | 2.37E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene34687 | 0.819496 | 1.13E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene34687 | 0.805483 | 1.31E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene34687 | 0.801229 | 9.94E-49 | -1.69E-46 |
| MS.gene060586 | MS.gene34687 | 0.841149 | 5.66E-58 | -1.69E-46 |
| MS.gene060683 | MS.gene34687 | 0.821194 | 4.60E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene34687 | 0.811866 | 5.65E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene34687 | 0.811394 | 7.16E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene34687 | 0.808827 | 2.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34687.t1 | MTR_8g055880 | 97.917 | 336 | 7 | 0 | 1 | 336 | 1 | 336 | 0.0 | 672 |
| MS.gene34687.t1 | MTR_8g055880 | 97.647 | 255 | 6 | 0 | 82 | 336 | 1 | 255 | 0.0 | 518 |
| MS.gene34687.t1 | MTR_8g055880 | 97.490 | 239 | 6 | 0 | 1 | 239 | 1 | 239 | 1.08e-170 | 476 |
| MS.gene34687.t1 | MTR_8g055880 | 97.899 | 238 | 5 | 0 | 1 | 238 | 1 | 238 | 2.81e-170 | 474 |
| MS.gene34687.t1 | MTR_8g055880 | 91.102 | 236 | 20 | 1 | 101 | 336 | 3 | 237 | 6.52e-155 | 435 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34687.t1 | AT1G14300 | 72.321 | 336 | 93 | 0 | 1 | 336 | 1 | 336 | 4.71e-178 | 498 |
| MS.gene34687.t1 | AT1G14300 | 65.241 | 374 | 92 | 1 | 1 | 336 | 1 | 374 | 1.09e-170 | 481 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATACTTGAGTGCGAATTTC+TGG | 0.188990 | 8.1:-45994549 | None:intergenic |
| TAACCGACTTGTACTATTCT+AGG | 0.244285 | 8.1:-45997276 | None:intergenic |
| CGAATTTCTGGATCATTAAC+AGG | 0.275821 | 8.1:-45994537 | None:intergenic |
| TCGTTTGCTTGTTATGCTTT+TGG | 0.276364 | 8.1:+45991810 | MS.gene34687:CDS |
| TCATACGCTCCCATCACTTT+TGG | 0.288944 | 8.1:-45997315 | None:intergenic |
| CTTTGTGATGTTAACAAGTA+TGG | 0.309114 | 8.1:-45992878 | None:intergenic |
| CATATACTTGATCTTATTGC+AGG | 0.326191 | 8.1:+45994578 | MS.gene34687:CDS |
| TGGTACTGATTCCTTGCTTC+AGG | 0.336668 | 8.1:+45991855 | MS.gene34687:CDS |
| TCAGCTACAGAATTTGCTTT+TGG | 0.342043 | 8.1:+45993691 | MS.gene34687:CDS |
| GACATAGTTCGCGGATTAAC+CGG | 0.348456 | 8.1:+45990706 | MS.gene34687:CDS |
| CTGCAATAAGATCAAGTATA+TGG | 0.360937 | 8.1:-45994577 | None:intergenic |
| CTGGCCTGCATTACTTCTTC+CGG | 0.363720 | 8.1:+45993727 | MS.gene34687:CDS |
| TGTTTCAAGAGACCCCGTTT+TGG | 0.375889 | 8.1:-45992931 | None:intergenic |
| GGCTTTGTCCCGTCTCTTAA+CGG | 0.395262 | 8.1:+45990780 | MS.gene34687:CDS |
| AGGCAGGTCGAAGAGCCTTT+TGG | 0.400322 | 8.1:+45997241 | MS.gene34687:CDS |
| CAAATTCTGTAGCTGATTCT+CGG | 0.403605 | 8.1:-45993684 | None:intergenic |
| TGATATTGCTATCAGGTCTC+TGG | 0.439026 | 8.1:+45993635 | MS.gene34687:intron |
| AGAGTGTATAACCTTGTTGC+CGG | 0.449596 | 8.1:-45993753 | None:intergenic |
| CACAAAGCAGCGGGCAGGGA+GGG | 0.451872 | 8.1:+45992894 | MS.gene34687:CDS |
| AACAGCTCATTGAGAAAGAA+AGG | 0.453988 | 8.1:+45992976 | MS.gene34687:CDS |
| ATCCAGTTGGTTAACGGCAG+CGG | 0.457409 | 8.1:+45997471 | MS.gene34687:intron |
| AACATCACAAAGCAGCGGGC+AGG | 0.468910 | 8.1:+45992889 | MS.gene34687:CDS |
| GTTGGTTAACGGCAGCGGTA+TGG | 0.481170 | 8.1:+45997476 | MS.gene34687:intron |
| ATCCTCAGCTCATTGCAAAC+AGG | 0.484449 | 8.1:+45998000 | MS.gene34687:CDS |
| AGAAATTCGCACTCAAGTAT+TGG | 0.484616 | 8.1:+45994551 | MS.gene34687:CDS |
| GCAAACAGGAATTCAAATGC+TGG | 0.487342 | 8.1:+45998014 | MS.gene34687:CDS |
| TTCGAATCTTGCGGAGGAGA+TGG | 0.490479 | 8.1:+45991726 | MS.gene34687:CDS |
| AGGCCCATTTACAGACCAAA+AGG | 0.505026 | 8.1:-45997256 | None:intergenic |
| CAAACAGGAATTCAAATGCT+GGG | 0.508789 | 8.1:+45998015 | MS.gene34687:CDS |
| ATAACCTTGTTGCCGGCTAC+CGG | 0.509222 | 8.1:-45993746 | None:intergenic |
| GTATGAGCAACTAGGATCCT+TGG | 0.516650 | 8.1:+45997332 | MS.gene34687:CDS |
| ATGTATGCCTTCCTCGGAGC+CGG | 0.517078 | 8.1:-45990725 | None:intergenic |
| ACAGCTCATTGAGAAAGAAA+GGG | 0.517237 | 8.1:+45992977 | MS.gene34687:CDS |
| AGGACGTACCGTCCTTCTCA+TGG | 0.518908 | 8.1:-45992442 | None:intergenic |
| CTTGGGCGCCGTTAAGAGAC+GGG | 0.520454 | 8.1:-45990788 | None:intergenic |
| GCATTACTTCTTCCGGTAGC+CGG | 0.527530 | 8.1:+45993734 | MS.gene34687:CDS |
| CCGTCTCTTAACGGCGCCCA+AGG | 0.529377 | 8.1:+45990789 | MS.gene34687:CDS |
| CGAGGAAGATCCAAAAGTGA+TGG | 0.532198 | 8.1:+45997305 | MS.gene34687:CDS |
| TACTCTGTCGTCTCAGTGGA+TGG | 0.536104 | 8.1:-45997504 | None:intergenic |
| CTTATCTTCTCCATCACCAC+AGG | 0.538535 | 8.1:+45990563 | MS.gene34687:CDS |
| CCTTGGGCGCCGTTAAGAGA+CGG | 0.553235 | 8.1:-45990789 | None:intergenic |
| TGATTGATTACCTGTGGTGA+TGG | 0.557068 | 8.1:-45990573 | None:intergenic |
| GGATTAACCGGCTCCGAGGA+AGG | 0.559140 | 8.1:+45990718 | MS.gene34687:CDS |
| AATCTCACTCAACTTGATGC+TGG | 0.559771 | 8.1:+45991835 | MS.gene34687:CDS |
| TAAAGAATGTATGCCTTCCT+CGG | 0.559797 | 8.1:-45990731 | None:intergenic |
| GCTACCGGAAGAAGTAATGC+AGG | 0.562221 | 8.1:-45993731 | None:intergenic |
| TTCGCAAAATTCGAATCTTG+CGG | 0.563174 | 8.1:+45991717 | MS.gene34687:CDS |
| ATTACATCGAAGTGTGGTGT+AGG | 0.566871 | 8.1:+45998054 | MS.gene34687:CDS |
| GTTGTTTCAGGAGGTTTCTG+AGG | 0.571869 | 8.1:+45991669 | MS.gene34687:intron |
| GGACGTACCGTCCTTCTCAT+GGG | 0.580643 | 8.1:-45992441 | None:intergenic |
| TTGTAGAACCACCCATGAGA+AGG | 0.584482 | 8.1:+45992430 | MS.gene34687:CDS |
| GTAGTAAGAAAGAATGGCGA+CGG | 0.584809 | 8.1:+45990518 | None:intergenic |
| GGCAGAATGAGAGTGTTTGA+TGG | 0.587800 | 8.1:+45998078 | MS.gene34687:CDS |
| GAGGAAGATCCAAAAGTGAT+GGG | 0.590608 | 8.1:+45997306 | MS.gene34687:CDS |
| ACATCACAAAGCAGCGGGCA+GGG | 0.590651 | 8.1:+45992890 | MS.gene34687:CDS |
| TGTTAACATCACAAAGCAGC+GGG | 0.593385 | 8.1:+45992885 | MS.gene34687:CDS |
| TATATGTTATGAAGCTTGTG+AGG | 0.597267 | 8.1:+45992402 | MS.gene34687:CDS |
| GCTGCCGTTGACATAGTTCG+CGG | 0.598021 | 8.1:+45990697 | MS.gene34687:CDS |
| AGTGCAGTCCCAAGTTCAAG+TGG | 0.605991 | 8.1:-45994498 | None:intergenic |
| ATGGGAGCGTATGAGCAACT+AGG | 0.606147 | 8.1:+45997324 | MS.gene34687:CDS |
| ATAAAAGCACACCTGAAGCA+AGG | 0.606782 | 8.1:-45991866 | None:intergenic |
| TTCCTGTTTGCAATGAGCTG+AGG | 0.615034 | 8.1:-45998002 | None:intergenic |
| TCACAAAGCAGCGGGCAGGG+AGG | 0.618149 | 8.1:+45992893 | MS.gene34687:CDS |
| TACGCAGATCTACAGTGAGG+AGG | 0.618557 | 8.1:+45994461 | MS.gene34687:intron |
| GGTTAACGGCAGCGGTATGG+AGG | 0.622742 | 8.1:+45997479 | MS.gene34687:CDS |
| TTGTCAGCTTGATGATGAGA+AGG | 0.623330 | 8.1:+45992370 | MS.gene34687:intron |
| AAACGAGTGACGCAACACTC+AGG | 0.629047 | 8.1:-45991793 | None:intergenic |
| TCTTCCGGTAGCCGGCAACA+AGG | 0.629059 | 8.1:+45993742 | MS.gene34687:CDS |
| CAGCTCATTGAGAAAGAAAG+GGG | 0.629207 | 8.1:+45992978 | MS.gene34687:CDS |
| ACATCGAAGTGTGGTGTAGG+CGG | 0.630686 | 8.1:+45998057 | MS.gene34687:CDS |
| TAACTACTCTGTCGTCTCAG+TGG | 0.638294 | 8.1:-45997508 | None:intergenic |
| GCAAAATTCGAATCTTGCGG+AGG | 0.645849 | 8.1:+45991720 | MS.gene34687:CDS |
| GAGACTTGTTGAAACAACAA+TGG | 0.650027 | 8.1:+45991756 | MS.gene34687:CDS |
| AAACAGGAATTCAAATGCTG+GGG | 0.656858 | 8.1:+45998016 | MS.gene34687:CDS |
| CGTACCGTCCTTCTCATGGG+TGG | 0.672425 | 8.1:-45992438 | None:intergenic |
| GGGCCTAGAATAGTACAAGT+CGG | 0.672654 | 8.1:+45997273 | MS.gene34687:CDS |
| CTTGATGATGAGAAGGTGCG+TGG | 0.673018 | 8.1:+45992377 | MS.gene34687:CDS |
| TAATCCGCGAACTATGTCAA+CGG | 0.679051 | 8.1:-45990701 | None:intergenic |
| CCGCTGATTACATCGAAGTG+TGG | 0.681488 | 8.1:+45998048 | MS.gene34687:CDS |
| TTGTTAACATCACAAAGCAG+CGG | 0.687062 | 8.1:+45992884 | MS.gene34687:CDS |
| TCGCGGATTAACCGGCTCCG+AGG | 0.690869 | 8.1:+45990714 | MS.gene34687:CDS |
| TTTACTGTTACAGATCACGA+AGG | 0.695135 | 8.1:+45990675 | MS.gene34687:intron |
| AGAACCACCCATGAGAAGGA+CGG | 0.701263 | 8.1:+45992434 | MS.gene34687:CDS |
| ACAAGTCGGTTATGAAGACG+AGG | 0.712076 | 8.1:+45997287 | MS.gene34687:CDS |
| CCACACTTCGATGTAATCAG+CGG | 0.726622 | 8.1:-45998048 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 45990531 | 45998097 | 45990531 | ID=MS.gene34687 |
| chr8.1 | mRNA | 45990531 | 45998097 | 45990531 | ID=MS.gene34687.t1;Parent=MS.gene34687 |
| chr8.1 | exon | 45990531 | 45990584 | 45990531 | ID=MS.gene34687.t1.exon1;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45990531 | 45990584 | 45990531 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45990688 | 45990810 | 45990688 | ID=MS.gene34687.t1.exon2;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45990688 | 45990810 | 45990688 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45991679 | 45991876 | 45991679 | ID=MS.gene34687.t1.exon3;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45991679 | 45991876 | 45991679 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45992377 | 45992455 | 45992377 | ID=MS.gene34687.t1.exon4;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45992377 | 45992455 | 45992377 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45992854 | 45992999 | 45992854 | ID=MS.gene34687.t1.exon5;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45992854 | 45992999 | 45992854 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45993650 | 45993763 | 45993650 | ID=MS.gene34687.t1.exon6;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45993650 | 45993763 | 45993650 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45994468 | 45994599 | 45994468 | ID=MS.gene34687.t1.exon7;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45994468 | 45994599 | 45994468 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45997240 | 45997353 | 45997240 | ID=MS.gene34687.t1.exon8;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45997240 | 45997353 | 45997240 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45997477 | 45997523 | 45997477 | ID=MS.gene34687.t1.exon9;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45997477 | 45997523 | 45997477 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| chr8.1 | exon | 45997986 | 45998097 | 45997986 | ID=MS.gene34687.t1.exon10;Parent=MS.gene34687.t1 |
| chr8.1 | CDS | 45997986 | 45998097 | 45997986 | ID=cds.MS.gene34687.t1;Parent=MS.gene34687.t1 |
| Gene Sequence |
| Protein sequence |
