Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35615.t1 | XP_013460856.1 | 95.1 | 326 | 15 | 1 | 1 | 326 | 1 | 325 | 9.00E-172 | 612.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35615.t1 | Q55CU8 | 25.6 | 246 | 175 | 4 | 20 | 257 | 43 | 288 | 8.6e-22 | 105.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35615.t1 | G8A376 | 95.1 | 326 | 15 | 1 | 1 | 326 | 1 | 325 | 6.5e-172 | 612.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene35615 | 0.846963 | 1.56E-59 | -1.69E-46 |
| MS.gene049369 | MS.gene35615 | 0.847472 | 1.13E-59 | -1.69E-46 |
| MS.gene049396 | MS.gene35615 | 0.84754 | 1.08E-59 | -1.69E-46 |
| MS.gene050520 | MS.gene35615 | 0.802933 | 4.43E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene35615 | 0.84451 | 7.23E-59 | -1.69E-46 |
| MS.gene050670 | MS.gene35615 | 0.838944 | 2.13E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene35615 | 0.820322 | 7.29E-53 | -1.69E-46 |
| MS.gene051360 | MS.gene35615 | 0.830527 | 2.79E-55 | -1.69E-46 |
| MS.gene051387 | MS.gene35615 | 0.811633 | 6.35E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene35615 | 0.801022 | 1.10E-48 | -1.69E-46 |
| MS.gene051874 | MS.gene35615 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene35615 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene35615 | 0.804033 | 2.62E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene35615 | 0.814814 | 1.27E-51 | -1.69E-46 |
| MS.gene052508 | MS.gene35615 | 0.81841 | 1.99E-52 | -1.69E-46 |
| MS.gene052510 | MS.gene35615 | 0.815386 | 9.50E-52 | -1.69E-46 |
| MS.gene052999 | MS.gene35615 | 0.818745 | 1.67E-52 | -1.69E-46 |
| MS.gene053169 | MS.gene35615 | 0.808552 | 2.93E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene35615 | 0.816718 | 4.79E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene35615 | 0.86608 | 3.70E-65 | -1.69E-46 |
| MS.gene053574 | MS.gene35615 | 0.80617 | 9.37E-50 | -1.69E-46 |
| MS.gene053813 | MS.gene35615 | 0.820765 | 5.77E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene35615 | 0.810621 | 1.05E-50 | -1.69E-46 |
| MS.gene05492 | MS.gene35615 | 0.812142 | 4.92E-51 | -1.69E-46 |
| MS.gene05505 | MS.gene35615 | 0.800267 | 1.56E-48 | -1.69E-46 |
| MS.gene055116 | MS.gene35615 | 0.815437 | 9.25E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene35615 | 0.806187 | 9.29E-50 | -1.69E-46 |
| MS.gene055666 | MS.gene35615 | 0.809714 | 1.65E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene35615 | 0.815872 | 7.40E-52 | -1.69E-46 |
| MS.gene055909 | MS.gene35615 | 0.822252 | 2.62E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene35615 | 0.821945 | 3.08E-53 | -1.69E-46 |
| MS.gene05592 | MS.gene35615 | 0.826984 | 2.01E-54 | -1.69E-46 |
| MS.gene056407 | MS.gene35615 | 0.815065 | 1.12E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene35615 | 0.805289 | 1.43E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene35615 | 0.891917 | 2.56E-74 | -1.69E-46 |
| MS.gene056448 | MS.gene35615 | 0.807674 | 4.50E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene35615 | 0.865147 | 7.29E-65 | -1.69E-46 |
| MS.gene05666 | MS.gene35615 | 0.802638 | 5.10E-49 | -1.69E-46 |
| MS.gene056833 | MS.gene35615 | 0.801507 | 8.72E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene35615 | 0.812318 | 4.50E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene35615 | 0.811627 | 6.37E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene35615 | 0.829269 | 5.66E-55 | -1.69E-46 |
| MS.gene05735 | MS.gene35615 | 0.800393 | 1.47E-48 | -1.69E-46 |
| MS.gene057448 | MS.gene35615 | 0.822074 | 2.88E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene35615 | 0.831455 | 1.65E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene35615 | 0.821127 | 4.76E-53 | -1.69E-46 |
| MS.gene057537 | MS.gene35615 | 0.818663 | 1.74E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene35615 | 0.856274 | 3.59E-62 | -1.69E-46 |
| MS.gene057856 | MS.gene35615 | 0.800182 | 1.62E-48 | -1.69E-46 |
| MS.gene057926 | MS.gene35615 | 0.813276 | 2.78E-51 | -1.69E-46 |
| MS.gene058075 | MS.gene35615 | 0.805026 | 1.63E-49 | -1.69E-46 |
| MS.gene05842 | MS.gene35615 | 0.824195 | 9.21E-54 | -1.69E-46 |
| MS.gene058459 | MS.gene35615 | 0.845786 | 3.27E-59 | -1.69E-46 |
| MS.gene058700 | MS.gene35615 | 0.823433 | 1.39E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene35615 | 0.807304 | 5.40E-50 | -1.69E-46 |
| MS.gene059366 | MS.gene35615 | 0.828344 | 9.47E-55 | -1.69E-46 |
| MS.gene059368 | MS.gene35615 | 0.812435 | 4.25E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene35615 | 0.820665 | 6.08E-53 | -1.69E-46 |
| MS.gene059517 | MS.gene35615 | 0.805 | 1.65E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene35615 | 0.816529 | 5.28E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene35615 | 0.81774 | 2.82E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene35615 | 0.849699 | 2.73E-60 | -1.69E-46 |
| MS.gene059807 | MS.gene35615 | 0.86619 | 3.42E-65 | -1.69E-46 |
| MS.gene059933 | MS.gene35615 | 0.810876 | 9.27E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene35615 | 0.821819 | 3.30E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene35615 | 0.839403 | 1.62E-57 | -1.69E-46 |
| MS.gene060804 | MS.gene35615 | 0.813669 | 2.28E-51 | -1.69E-46 |
| MS.gene060818 | MS.gene35615 | 0.815492 | 8.99E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene35615 | 0.815026 | 1.14E-51 | -1.69E-46 |
| MS.gene060837 | MS.gene35615 | 0.892647 | 1.31E-74 | -1.69E-46 |
| MS.gene061051 | MS.gene35615 | -0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene35615 | 0.841846 | 3.71E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35615.t1 | MTR_3g074050 | 95.092 | 326 | 15 | 1 | 1 | 326 | 1 | 325 | 0.0 | 645 |
| MS.gene35615.t1 | MTR_3g074050 | 94.785 | 326 | 15 | 2 | 1 | 326 | 1 | 324 | 0.0 | 639 |
| MS.gene35615.t1 | MTR_3g074050 | 93.798 | 258 | 15 | 1 | 69 | 326 | 1 | 257 | 0.0 | 509 |
| MS.gene35615.t1 | MTR_5g062310 | 65.472 | 307 | 103 | 1 | 1 | 307 | 1 | 304 | 1.65e-149 | 423 |
| MS.gene35615.t1 | MTR_5g062310 | 64.854 | 239 | 84 | 0 | 69 | 307 | 1 | 239 | 6.86e-116 | 336 |
| MS.gene35615.t1 | MTR_7g087500 | 43.793 | 290 | 156 | 2 | 14 | 303 | 12 | 294 | 3.59e-91 | 275 |
| MS.gene35615.t1 | MTR_8g077700 | 48.400 | 250 | 128 | 1 | 22 | 270 | 16 | 265 | 1.26e-84 | 257 |
| MS.gene35615.t1 | MTR_8g077700 | 48.400 | 250 | 128 | 1 | 22 | 270 | 44 | 293 | 9.78e-84 | 256 |
| MS.gene35615.t1 | MTR_1g022185 | 44.715 | 246 | 135 | 1 | 26 | 270 | 20 | 265 | 5.88e-78 | 240 |
| MS.gene35615.t1 | MTR_4g105450 | 28.402 | 169 | 118 | 2 | 52 | 218 | 72 | 239 | 1.65e-23 | 97.8 |
| MS.gene35615.t1 | MTR_8g096180 | 29.825 | 228 | 153 | 7 | 20 | 244 | 19 | 242 | 3.34e-23 | 96.7 |
| MS.gene35615.t1 | MTR_4g105460 | 29.299 | 157 | 109 | 1 | 55 | 209 | 77 | 233 | 4.53e-22 | 94.0 |
| MS.gene35615.t1 | MTR_8g096170 | 29.075 | 227 | 154 | 7 | 21 | 244 | 23 | 245 | 2.73e-20 | 89.0 |
| MS.gene35615.t1 | MTR_8g096180 | 28.571 | 238 | 153 | 8 | 20 | 244 | 19 | 252 | 6.12e-20 | 88.2 |
| MS.gene35615.t1 | MTR_3g098530 | 26.549 | 226 | 159 | 7 | 22 | 244 | 41 | 262 | 1.19e-19 | 87.4 |
| MS.gene35615.t1 | MTR_1g104540 | 28.358 | 201 | 137 | 3 | 21 | 215 | 8 | 207 | 1.57e-18 | 85.9 |
| MS.gene35615.t1 | MTR_4g105460 | 29.104 | 134 | 89 | 3 | 55 | 185 | 77 | 207 | 1.42e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35615.t1 | AT4G08690 | 54.098 | 244 | 112 | 0 | 22 | 265 | 16 | 259 | 3.92e-104 | 307 |
| MS.gene35615.t1 | AT4G08690 | 54.098 | 244 | 112 | 0 | 22 | 265 | 16 | 259 | 3.92e-104 | 307 |
| MS.gene35615.t1 | AT4G08690 | 54.098 | 244 | 112 | 0 | 22 | 265 | 16 | 259 | 3.92e-104 | 307 |
| MS.gene35615.t1 | AT1G22180 | 50.654 | 306 | 136 | 5 | 12 | 311 | 12 | 308 | 2.43e-103 | 306 |
| MS.gene35615.t1 | AT1G22180 | 50.654 | 306 | 136 | 5 | 12 | 311 | 12 | 308 | 2.43e-103 | 306 |
| MS.gene35615.t1 | AT1G22180 | 51.004 | 249 | 110 | 4 | 69 | 311 | 1 | 243 | 9.31e-85 | 256 |
| MS.gene35615.t1 | AT1G22180 | 51.004 | 249 | 110 | 4 | 69 | 311 | 1 | 243 | 9.31e-85 | 256 |
| MS.gene35615.t1 | AT1G75170 | 43.411 | 258 | 145 | 1 | 25 | 281 | 20 | 277 | 4.52e-80 | 246 |
| MS.gene35615.t1 | AT1G75170 | 43.411 | 258 | 145 | 1 | 25 | 281 | 20 | 277 | 4.52e-80 | 246 |
| MS.gene35615.t1 | AT4G36640 | 41.573 | 267 | 155 | 1 | 24 | 289 | 16 | 282 | 4.31e-76 | 235 |
| MS.gene35615.t1 | AT4G36640 | 41.573 | 267 | 155 | 1 | 24 | 289 | 16 | 282 | 4.31e-76 | 235 |
| MS.gene35615.t1 | AT4G36640 | 40.991 | 222 | 130 | 1 | 69 | 289 | 1 | 222 | 1.20e-59 | 191 |
| MS.gene35615.t1 | AT1G75170 | 39.896 | 193 | 115 | 1 | 90 | 281 | 2 | 194 | 8.49e-49 | 162 |
| MS.gene35615.t1 | AT5G63060 | 31.410 | 156 | 105 | 1 | 55 | 208 | 78 | 233 | 3.09e-22 | 94.4 |
| MS.gene35615.t1 | AT5G63060 | 31.447 | 159 | 107 | 1 | 52 | 208 | 40 | 198 | 4.19e-22 | 93.2 |
| MS.gene35615.t1 | AT1G01630 | 27.347 | 245 | 171 | 7 | 3 | 244 | 5 | 245 | 7.60e-19 | 84.7 |
| MS.gene35615.t1 | AT1G05370 | 25.472 | 212 | 151 | 3 | 6 | 215 | 3 | 209 | 8.99e-15 | 75.1 |
| MS.gene35615.t1 | AT3G22410 | 27.189 | 217 | 142 | 6 | 24 | 225 | 6 | 221 | 1.78e-13 | 71.2 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 4.67e-11 | 63.9 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 4.67e-11 | 63.9 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 4.67e-11 | 63.9 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 4.67e-11 | 63.9 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 5.17e-11 | 63.9 |
| MS.gene35615.t1 | AT2G21540 | 28.638 | 213 | 127 | 9 | 54 | 245 | 98 | 306 | 5.17e-11 | 63.9 |
Find 65 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTCCTGTCTCCATTTC+AGG | 0.161025 | 3.3:+63868261 | None:intergenic |
| GGTATTTAAAATCACAAAAT+TGG | 0.173573 | 3.3:-63868319 | MS.gene35615:CDS |
| CCATAATCTTCTTTGTGTTT+TGG | 0.230567 | 3.3:+63867365 | None:intergenic |
| CCATCCTTGGCACAATAATT+TGG | 0.234391 | 3.3:+63868003 | None:intergenic |
| TATGGAGGGTTTGTTTGATA+TGG | 0.249989 | 3.3:-63867347 | MS.gene35615:CDS |
| ATAACAAGATGCACTCCTTT+TGG | 0.282527 | 3.3:-63867243 | MS.gene35615:CDS |
| CTTTATCTTATTATAATTCT+CGG | 0.306203 | 3.3:+63867405 | None:intergenic |
| CTTACCCAGGTAATCTCTTC+AGG | 0.306944 | 3.3:+63868236 | None:intergenic |
| CCAAAACACAAAGAAGATTA+TGG | 0.309315 | 3.3:-63867365 | MS.gene35615:CDS |
| GAGATATATTGATCAGGTTT+AGG | 0.320441 | 3.3:+63867106 | None:intergenic |
| CTCACCAGGAGCAGGTGATC+TGG | 0.403831 | 3.3:-63867760 | MS.gene35615:CDS |
| TTTGTTCTCATTATAAGTAC+TGG | 0.408192 | 3.3:+63867976 | None:intergenic |
| GTTCTGATGCATCCATTTCA+AGG | 0.412879 | 3.3:-63868340 | MS.gene35615:CDS |
| ATCTGGCTGGTTGATTTCCA+TGG | 0.440350 | 3.3:-63867743 | MS.gene35615:CDS |
| GATCAACTTGAACCTGCATT+TGG | 0.447521 | 3.3:-63867325 | MS.gene35615:CDS |
| AAGTACTGGTCTTCCATCCT+TGG | 0.473632 | 3.3:+63867990 | None:intergenic |
| GTTGCTAATGAAGCAGAGAC+AGG | 0.477872 | 3.3:-63868039 | MS.gene35615:CDS |
| TATTTCGTTACCGAGAAGAA+TGG | 0.481590 | 3.3:+63867602 | None:intergenic |
| ATTGAGGTGAGAAAGTTGAT+AGG | 0.483915 | 3.3:-63868392 | MS.gene35615:CDS |
| TTTAAACCTTCCACCTCACC+AGG | 0.488686 | 3.3:-63867774 | MS.gene35615:CDS |
| GTAACTGAGATATATTGATC+AGG | 0.500083 | 3.3:+63867100 | None:intergenic |
| CCAGGAGCAGGTGATCTGGC+TGG | 0.500834 | 3.3:-63867756 | MS.gene35615:CDS |
| CCAAATTATTGTGCCAAGGA+TGG | 0.506258 | 3.3:-63868003 | MS.gene35615:CDS |
| TCATTATTCCCACCAAATGC+AGG | 0.506468 | 3.3:+63867313 | None:intergenic |
| CCTGCTCCTGGTGAGGTGGA+AGG | 0.508219 | 3.3:+63867768 | None:intergenic |
| CAACTTGAACCTGCATTTGG+TGG | 0.510649 | 3.3:-63867322 | MS.gene35615:CDS |
| AGAGTTTGCATGTGTCCAAA+AGG | 0.518082 | 3.3:+63867228 | None:intergenic |
| ATCACCTGCTCCTGGTGAGG+TGG | 0.523818 | 3.3:+63867764 | None:intergenic |
| TTATCCACAACGCCTCGGAT+TGG | 0.529118 | 3.3:-63867657 | MS.gene35615:CDS |
| CTGAAGAAAACTGCCTCTGA+TGG | 0.531528 | 3.3:-63868570 | MS.gene35615:CDS |
| TTCCTCCTTCAGATTTGACC+AGG | 0.531700 | 3.3:-63867183 | MS.gene35615:CDS |
| CACTTGACAAACTGAAACCA+TGG | 0.532083 | 3.3:+63867726 | None:intergenic |
| CCAGCCAGATCACCTGCTCC+TGG | 0.532714 | 3.3:+63867756 | None:intergenic |
| ATAAACCTGAAGAGATTACC+TGG | 0.535260 | 3.3:-63868241 | MS.gene35615:CDS |
| GAAGAATGGTTGGAAAATCC+CGG | 0.535429 | 3.3:+63867616 | None:intergenic |
| GATAGGAACATTATCAGATA+AGG | 0.538137 | 3.3:-63868375 | MS.gene35615:CDS |
| CAGATCACCTGCTCCTGGTG+AGG | 0.548938 | 3.3:+63867761 | None:intergenic |
| ATATGCTAAGAGAATGAGAG+AGG | 0.549993 | 3.3:-63867269 | MS.gene35615:CDS |
| AACTTGAACCTGCATTTGGT+GGG | 0.550189 | 3.3:-63867321 | MS.gene35615:CDS |
| TATAATGAGAACAAATCGCC+AGG | 0.555150 | 3.3:-63867968 | MS.gene35615:intron |
| CCTTCCACCTCACCAGGAGC+AGG | 0.557345 | 3.3:-63867768 | MS.gene35615:CDS |
| AATATATCTCAGTTACTCGC+GGG | 0.560385 | 3.3:-63867093 | MS.gene35615:CDS |
| ATCAGAATCTGCTACTAACC+TGG | 0.564891 | 3.3:+63867165 | None:intergenic |
| GCAATCATGTATGATGCACC+CGG | 0.576986 | 3.3:-63867635 | MS.gene35615:CDS |
| ATTGCCAATCCGAGGCGTTG+TGG | 0.577592 | 3.3:+63867653 | None:intergenic |
| AAATATTATCCACAACGCCT+CGG | 0.583955 | 3.3:-63867662 | MS.gene35615:CDS |
| TGGTGCATTCCAGATCATTG+AGG | 0.589779 | 3.3:-63868408 | MS.gene35615:intron |
| TCGTTACCGAGAAGAATGGT+TGG | 0.590066 | 3.3:+63867606 | None:intergenic |
| AACACAAAGAAGATTATGGA+GGG | 0.591841 | 3.3:-63867361 | MS.gene35615:CDS |
| ACATTGTGTGAGACTGATGA+TGG | 0.595579 | 3.3:+63867205 | None:intergenic |
| AAGAATGGTTGGAAAATCCC+GGG | 0.597907 | 3.3:+63867617 | None:intergenic |
| ACTAACCTGGTCAAATCTGA+AGG | 0.603314 | 3.3:+63867178 | None:intergenic |
| TGAATTGTTGCAGATGGTGA+AGG | 0.607257 | 3.3:-63867440 | MS.gene35615:intron |
| TAGACCAAATTATTGTGCCA+AGG | 0.612792 | 3.3:-63868007 | MS.gene35615:CDS |
| AAACACAAAGAAGATTATGG+AGG | 0.618726 | 3.3:-63867362 | MS.gene35615:CDS |
| TCGCGGGGACACAAAGGTCA+TGG | 0.619216 | 3.3:-63867077 | MS.gene35615:CDS |
| GTCACAAGAACAGCAAGCAA+AGG | 0.621333 | 3.3:-63868520 | MS.gene35615:intron |
| CAATATATCTCAGTTACTCG+CGG | 0.625172 | 3.3:-63867094 | MS.gene35615:CDS |
| AGTTACTCGCGGGGACACAA+AGG | 0.631514 | 3.3:-63867083 | MS.gene35615:CDS |
| AACCTGGTCAAATCTGAAGG+AGG | 0.655670 | 3.3:+63867181 | None:intergenic |
| TTGCTAATGAAGCAGAGACA+GGG | 0.659274 | 3.3:-63868038 | MS.gene35615:CDS |
| CAATCATGTATGATGCACCC+GGG | 0.666450 | 3.3:-63867634 | MS.gene35615:CDS |
| CATACATGATTGCCAATCCG+AGG | 0.671750 | 3.3:+63867645 | None:intergenic |
| TAAACCTGAAGAGATTACCT+GGG | 0.681766 | 3.3:-63868240 | MS.gene35615:intron |
| ATATATCTCAGTTACTCGCG+GGG | 0.688008 | 3.3:-63867092 | MS.gene35615:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAATATCAACATTTTA+CGG | + | chr3.3:63868132-63868151 | None:intergenic | 10.0% |
| !!! | GATAATATTTTTGTAATATA+TGG | + | chr3.3:63867982-63868001 | None:intergenic | 10.0% |
| !! | CTTTATCTTATTATAATTCT+CGG | + | chr3.3:63868252-63868271 | None:intergenic | 15.0% |
| !! | TAGCTGATAATAGTTTAATT+TGG | - | chr3.3:63867226-63867245 | MS.gene35615:CDS | 20.0% |
| !!! | GGTATTTAAAATCACAAAAT+TGG | - | chr3.3:63867335-63867354 | MS.gene35615:CDS | 20.0% |
| !!! | TGATTTTAAATACCTTGAAA+TGG | + | chr3.3:63867329-63867348 | None:intergenic | 20.0% |
| !!! | TTAACAAGTATGTTTTTTCT+TGG | - | chr3.3:63867581-63867600 | MS.gene35615:intron | 20.0% |
| ! | AACAAAGCCTAAAATTGTTA+TGG | - | chr3.3:63867487-63867506 | MS.gene35615:intron | 25.0% |
| ! | AGAACATTGATCAAACTTAT+CGG | + | chr3.3:63868169-63868188 | None:intergenic | 25.0% |
| ! | ATGTCCAATAATTGAAATCT+TGG | - | chr3.3:63867158-63867177 | MS.gene35615:CDS | 25.0% |
| ! | TTTGTTCTCATTATAAGTAC+TGG | + | chr3.3:63867681-63867700 | None:intergenic | 25.0% |
| !!! | AACAACAATATTTTGAGTGA+AGG | - | chr3.3:63867197-63867216 | MS.gene35615:CDS | 25.0% |
| !!! | AGTATGTTTTTTCTTGGTTA+AGG | - | chr3.3:63867587-63867606 | MS.gene35615:intron | 25.0% |
| !!! | TTTTTTTTTACAGCAGAAGT+TGG | - | chr3.3:63868104-63868123 | MS.gene35615:intron | 25.0% |
| AAACACAAAGAAGATTATGG+AGG | - | chr3.3:63868292-63868311 | MS.gene35615:CDS | 30.0% | |
| AAAGAATGAATTGTTGCAGA+TGG | - | chr3.3:63868208-63868227 | MS.gene35615:intron | 30.0% | |
| AACACAAAGAAGATTATGGA+GGG | - | chr3.3:63868293-63868312 | MS.gene35615:CDS | 30.0% | |
| CCAAAACACAAAGAAGATTA+TGG | - | chr3.3:63868289-63868308 | MS.gene35615:CDS | 30.0% | |
| GAGATATATTGATCAGGTTT+AGG | + | chr3.3:63868551-63868570 | None:intergenic | 30.0% | |
| GATAGGAACATTATCAGATA+AGG | - | chr3.3:63867279-63867298 | MS.gene35615:CDS | 30.0% | |
| GTAACTGAGATATATTGATC+AGG | + | chr3.3:63868557-63868576 | None:intergenic | 30.0% | |
| TCAGATTGAATTGAAAGATG+AGG | - | chr3.3:63867527-63867546 | MS.gene35615:intron | 30.0% | |
| TGCTCCAAGATTTCAATTAT+TGG | + | chr3.3:63867165-63867184 | None:intergenic | 30.0% | |
| TGTTGAAAAAAAGCCTGAAA+TGG | - | chr3.3:63867380-63867399 | MS.gene35615:CDS | 30.0% | |
| ! | AACACAGCCATAACAATTTT+AGG | + | chr3.3:63867497-63867516 | None:intergenic | 30.0% |
| ! | CCATAATCTTCTTTGTGTTT+TGG | + | chr3.3:63868292-63868311 | None:intergenic | 30.0% |
| !! | AATATTTTGAGTGAAGGATG+AGG | - | chr3.3:63867203-63867222 | MS.gene35615:CDS | 30.0% |
| !! | TAAGCATTTTGTTTACTGCA+TGG | - | chr3.3:63867847-63867866 | MS.gene35615:intron | 30.0% |
| AAATATTATCCACAACGCCT+CGG | - | chr3.3:63867992-63868011 | MS.gene35615:CDS | 35.0% | |
| AATATATCTCAGTTACTCGC+GGG | - | chr3.3:63868561-63868580 | MS.gene35615:CDS | 35.0% | |
| ACTTCAGTACGAGTGAAAAA+TGG | - | chr3.3:63867748-63867767 | MS.gene35615:CDS | 35.0% | |
| ATAAACCTGAAGAGATTACC+TGG | - | chr3.3:63867413-63867432 | MS.gene35615:CDS | 35.0% | |
| ATATGCTAAGAGAATGAGAG+AGG | - | chr3.3:63868385-63868404 | MS.gene35615:CDS | 35.0% | |
| CAATATATCTCAGTTACTCG+CGG | - | chr3.3:63868560-63868579 | MS.gene35615:CDS | 35.0% | |
| TAAACCTGAAGAGATTACCT+GGG | - | chr3.3:63867414-63867433 | MS.gene35615:CDS | 35.0% | |
| TAGACCAAATTATTGTGCCA+AGG | - | chr3.3:63867647-63867666 | MS.gene35615:CDS | 35.0% | |
| TATAATGAGAACAAATCGCC+AGG | - | chr3.3:63867686-63867705 | MS.gene35615:CDS | 35.0% | |
| TATATTCCTGTCTCCATTTC+AGG | + | chr3.3:63867396-63867415 | None:intergenic | 35.0% | |
| TATTTCGTTACCGAGAAGAA+TGG | + | chr3.3:63868055-63868074 | None:intergenic | 35.0% | |
| ! | ACACTGCTAACATTTTCTCA+TGG | + | chr3.3:63867110-63867129 | None:intergenic | 35.0% |
| ! | AGTGATTTTCAGAAGAAACC+TGG | + | chr3.3:63867707-63867726 | None:intergenic | 35.0% |
| ! | ATAACAAGATGCACTCCTTT+TGG | - | chr3.3:63868411-63868430 | MS.gene35615:intron | 35.0% |
| ! | ATCAACTATTTTCTCGTCGT+TGG | + | chr3.3:63868519-63868538 | None:intergenic | 35.0% |
| ! | ATTGAGGTGAGAAAGTTGAT+AGG | - | chr3.3:63867262-63867281 | MS.gene35615:CDS | 35.0% |
| ! | GATTTTCCAACCATTCTTCT+CGG | - | chr3.3:63868042-63868061 | MS.gene35615:CDS | 35.0% |
| ! | TATGGAGGGTTTGTTTGATA+TGG | - | chr3.3:63868307-63868326 | MS.gene35615:CDS | 35.0% |
| AAAAAGCCTGAAATGGAGAC+AGG | - | chr3.3:63867387-63867406 | MS.gene35615:CDS | 40.0% | |
| AACTTGAACCTGCATTTGGT+GGG | - | chr3.3:63868333-63868352 | MS.gene35615:CDS | 40.0% | |
| AAGAATGGTTGGAAAATCCC+GGG | + | chr3.3:63868040-63868059 | None:intergenic | 40.0% | |
| ACATTGTGTGAGACTGATGA+TGG | + | chr3.3:63868452-63868471 | None:intergenic | 40.0% | |
| ACTAACCTGGTCAAATCTGA+AGG | + | chr3.3:63868479-63868498 | None:intergenic | 40.0% | |
| ACTTTCTCACCTCAATGATC+TGG | + | chr3.3:63867258-63867277 | None:intergenic | 40.0% | |
| AGAGTTTGCATGTGTCCAAA+AGG | + | chr3.3:63868429-63868448 | None:intergenic | 40.0% | |
| ATATATCTCAGTTACTCGCG+GGG | - | chr3.3:63868562-63868581 | MS.gene35615:CDS | 40.0% | |
| ATCAGAATCTGCTACTAACC+TGG | + | chr3.3:63868492-63868511 | None:intergenic | 40.0% | |
| CACTTGACAAACTGAAACCA+TGG | + | chr3.3:63867931-63867950 | None:intergenic | 40.0% | |
| CCAAATTATTGTGCCAAGGA+TGG | - | chr3.3:63867651-63867670 | MS.gene35615:CDS | 40.0% | |
| CCATCCTTGGCACAATAATT+TGG | + | chr3.3:63867654-63867673 | None:intergenic | 40.0% | |
| GAAGAATGGTTGGAAAATCC+CGG | + | chr3.3:63868041-63868060 | None:intergenic | 40.0% | |
| GATCAACTTGAACCTGCATT+TGG | - | chr3.3:63868329-63868348 | MS.gene35615:CDS | 40.0% | |
| GTTCTGATGCATCCATTTCA+AGG | - | chr3.3:63867314-63867333 | MS.gene35615:CDS | 40.0% | |
| TCATTATTCCCACCAAATGC+AGG | + | chr3.3:63868344-63868363 | None:intergenic | 40.0% | |
| TGAATTGTTGCAGATGGTGA+AGG | - | chr3.3:63868214-63868233 | MS.gene35615:intron | 40.0% | |
| TTGCTAATGAAGCAGAGACA+GGG | - | chr3.3:63867616-63867635 | MS.gene35615:CDS | 40.0% | |
| AACCTGGTCAAATCTGAAGG+AGG | + | chr3.3:63868476-63868495 | None:intergenic | 45.0% | |
| AAGCAAGCAGTAACTTACCC+AGG | + | chr3.3:63867434-63867453 | None:intergenic | 45.0% | |
| CAACTTGAACCTGCATTTGG+TGG | - | chr3.3:63868332-63868351 | MS.gene35615:CDS | 45.0% | |
| CAATCATGTATGATGCACCC+GGG | - | chr3.3:63868020-63868039 | MS.gene35615:CDS | 45.0% | |
| CATACATGATTGCCAATCCG+AGG | + | chr3.3:63868012-63868031 | None:intergenic | 45.0% | |
| CTGAAGAAAACTGCCTCTGA+TGG | - | chr3.3:63867084-63867103 | MS.gene35615:CDS | 45.0% | |
| CTTACCCAGGTAATCTCTTC+AGG | + | chr3.3:63867421-63867440 | None:intergenic | 45.0% | |
| GCAATCATGTATGATGCACC+CGG | - | chr3.3:63868019-63868038 | MS.gene35615:CDS | 45.0% | |
| GTCACAAGAACAGCAAGCAA+AGG | - | chr3.3:63867134-63867153 | MS.gene35615:CDS | 45.0% | |
| GTTGCTAATGAAGCAGAGAC+AGG | - | chr3.3:63867615-63867634 | MS.gene35615:CDS | 45.0% | |
| TCGTTACCGAGAAGAATGGT+TGG | + | chr3.3:63868051-63868070 | None:intergenic | 45.0% | |
| TGGTGCATTCCAGATCATTG+AGG | - | chr3.3:63867246-63867265 | MS.gene35615:CDS | 45.0% | |
| TTCCTCCTTCAGATTTGACC+AGG | - | chr3.3:63868471-63868490 | MS.gene35615:intron | 45.0% | |
| TTTAAACCTTCCACCTCACC+AGG | - | chr3.3:63867880-63867899 | MS.gene35615:intron | 45.0% | |
| ! | ATCTGGCTGGTTGATTTCCA+TGG | - | chr3.3:63867911-63867930 | MS.gene35615:intron | 45.0% |
| ! | CATTTTCTCATGGCCATCAG+AGG | + | chr3.3:63867100-63867119 | None:intergenic | 45.0% |
| !! | AAGTACTGGTCTTCCATCCT+TGG | + | chr3.3:63867667-63867686 | None:intergenic | 45.0% |
| TTATCCACAACGCCTCGGAT+TGG | - | chr3.3:63867997-63868016 | MS.gene35615:CDS | 50.0% | |
| AGTTACTCGCGGGGACACAA+AGG | - | chr3.3:63868571-63868590 | MS.gene35615:CDS | 55.0% | |
| !! | ATTGCCAATCCGAGGCGTTG+TGG | + | chr3.3:63868004-63868023 | None:intergenic | 55.0% |
| ATCACCTGCTCCTGGTGAGG+TGG | + | chr3.3:63867893-63867912 | None:intergenic | 60.0% | |
| CAGATCACCTGCTCCTGGTG+AGG | + | chr3.3:63867896-63867915 | None:intergenic | 60.0% | |
| CTCACCAGGAGCAGGTGATC+TGG | - | chr3.3:63867894-63867913 | MS.gene35615:intron | 60.0% | |
| TCGCGGGGACACAAAGGTCA+TGG | - | chr3.3:63868577-63868596 | MS.gene35615:CDS | 60.0% | |
| CCAGCCAGATCACCTGCTCC+TGG | + | chr3.3:63867901-63867920 | None:intergenic | 65.0% | |
| CCAGGAGCAGGTGATCTGGC+TGG | - | chr3.3:63867898-63867917 | MS.gene35615:intron | 65.0% | |
| CCTGCTCCTGGTGAGGTGGA+AGG | + | chr3.3:63867889-63867908 | None:intergenic | 65.0% | |
| CCTTCCACCTCACCAGGAGC+AGG | - | chr3.3:63867886-63867905 | MS.gene35615:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 63867072 | 63868604 | 63867072 | ID=MS.gene35615 |
| chr3.3 | mRNA | 63867072 | 63868604 | 63867072 | ID=MS.gene35615.t1;Parent=MS.gene35615 |
| chr3.3 | exon | 63868521 | 63868604 | 63868521 | ID=MS.gene35615.t1.exon1;Parent=MS.gene35615.t1 |
| chr3.3 | CDS | 63868521 | 63868604 | 63868521 | ID=cds.MS.gene35615.t1;Parent=MS.gene35615.t1 |
| chr3.3 | exon | 63868241 | 63868417 | 63868241 | ID=MS.gene35615.t1.exon2;Parent=MS.gene35615.t1 |
| chr3.3 | CDS | 63868241 | 63868417 | 63868241 | ID=cds.MS.gene35615.t1;Parent=MS.gene35615.t1 |
| chr3.3 | exon | 63867969 | 63868067 | 63867969 | ID=MS.gene35615.t1.exon3;Parent=MS.gene35615.t1 |
| chr3.3 | CDS | 63867969 | 63868067 | 63867969 | ID=cds.MS.gene35615.t1;Parent=MS.gene35615.t1 |
| chr3.3 | exon | 63867613 | 63867855 | 63867613 | ID=MS.gene35615.t1.exon4;Parent=MS.gene35615.t1 |
| chr3.3 | CDS | 63867613 | 63867855 | 63867613 | ID=cds.MS.gene35615.t1;Parent=MS.gene35615.t1 |
| chr3.3 | exon | 63867072 | 63867449 | 63867072 | ID=MS.gene35615.t1.exon5;Parent=MS.gene35615.t1 |
| chr3.3 | CDS | 63867072 | 63867449 | 63867072 | ID=cds.MS.gene35615.t1;Parent=MS.gene35615.t1 |
| Gene Sequence |
| Protein sequence |
