Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41086.t1 | XP_013467905.1 | 99.3 | 149 | 1 | 0 | 5 | 153 | 720 | 868 | 1.50E-76 | 295.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41086.t1 | P93422 | 60.0 | 150 | 56 | 3 | 5 | 153 | 642 | 788 | 9.2e-43 | 174.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41086.t1 | A0A072VJI1 | 99.3 | 149 | 1 | 0 | 5 | 153 | 720 | 868 | 1.1e-76 | 295.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049140 | MS.gene41086 | 0.805285 | 1.44E-49 | -1.69E-46 |
| MS.gene049580 | MS.gene41086 | 0.860139 | 2.54E-63 | -1.69E-46 |
| MS.gene049825 | MS.gene41086 | 0.830339 | 3.10E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene41086 | 0.80408 | 2.56E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene41086 | 0.835739 | 1.41E-56 | -1.69E-46 |
| MS.gene050752 | MS.gene41086 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene41086 | 0.835637 | 1.49E-56 | -1.69E-46 |
| MS.gene051003 | MS.gene41086 | 0.827608 | 1.42E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene41086 | 0.804325 | 2.28E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene41086 | 0.810708 | 1.01E-50 | -1.69E-46 |
| MS.gene051365 | MS.gene41086 | 0.809415 | 1.91E-50 | -1.69E-46 |
| MS.gene051700 | MS.gene41086 | 0.814803 | 1.28E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene41086 | 0.830538 | 2.78E-55 | -1.69E-46 |
| MS.gene051748 | MS.gene41086 | 0.826912 | 2.09E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene41086 | 0.83642 | 9.46E-57 | -1.69E-46 |
| MS.gene051902 | MS.gene41086 | 0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene051903 | MS.gene41086 | 0.83568 | 1.46E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene41086 | 0.818495 | 1.90E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene41086 | 0.854495 | 1.19E-61 | -1.69E-46 |
| MS.gene053045 | MS.gene41086 | 0.801085 | 1.06E-48 | -1.69E-46 |
| MS.gene053058 | MS.gene41086 | 0.830345 | 3.09E-55 | -1.69E-46 |
| MS.gene053298 | MS.gene41086 | 0.815645 | 8.31E-52 | -1.69E-46 |
| MS.gene053677 | MS.gene41086 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene053786 | MS.gene41086 | 0.810904 | 9.14E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene41086 | 0.81628 | 6.00E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene41086 | 0.804038 | 2.62E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene41086 | 0.806843 | 6.76E-50 | -1.69E-46 |
| MS.gene055555 | MS.gene41086 | 0.855749 | 5.12E-62 | -1.69E-46 |
| MS.gene055808 | MS.gene41086 | 0.822901 | 1.85E-53 | -1.69E-46 |
| MS.gene056043 | MS.gene41086 | -0.803609 | 3.21E-49 | -1.69E-46 |
| MS.gene056075 | MS.gene41086 | 0.809541 | 1.80E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene41086 | 0.818375 | 2.03E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene41086 | 0.831741 | 1.41E-55 | -1.69E-46 |
| MS.gene056605 | MS.gene41086 | 0.825127 | 5.55E-54 | -1.69E-46 |
| MS.gene056631 | MS.gene41086 | 0.837841 | 4.10E-57 | -1.69E-46 |
| MS.gene05689 | MS.gene41086 | 0.812051 | 5.15E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene41086 | 0.831246 | 1.86E-55 | -1.69E-46 |
| MS.gene057094 | MS.gene41086 | 0.822146 | 2.77E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene41086 | 0.810586 | 1.07E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene41086 | 0.819539 | 1.10E-52 | -1.69E-46 |
| MS.gene057705 | MS.gene41086 | 0.846231 | 2.47E-59 | -1.69E-46 |
| MS.gene05781 | MS.gene41086 | 0.832858 | 7.43E-56 | -1.69E-46 |
| MS.gene057999 | MS.gene41086 | 0.810708 | 1.01E-50 | -1.69E-46 |
| MS.gene058074 | MS.gene41086 | 0.804529 | 2.07E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene41086 | 0.806044 | 9.96E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene41086 | 0.825196 | 5.35E-54 | -1.69E-46 |
| MS.gene05954 | MS.gene41086 | 0.811802 | 5.83E-51 | -1.69E-46 |
| MS.gene059935 | MS.gene41086 | 0.802838 | 4.64E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene41086 | 0.843226 | 1.60E-58 | -1.69E-46 |
| MS.gene06011 | MS.gene41086 | 0.860801 | 1.60E-63 | -1.69E-46 |
| MS.gene060365 | MS.gene41086 | 0.806503 | 7.97E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene41086 | 0.807473 | 4.97E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene41086 | 0.864881 | 8.84E-65 | -1.69E-46 |
| MS.gene060830 | MS.gene41086 | 0.836663 | 8.20E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41086.t1 | MTR_1g057860 | 99.329 | 149 | 1 | 0 | 5 | 153 | 720 | 868 | 7.86e-101 | 308 |
| MS.gene41086.t1 | MTR_5g091840 | 53.659 | 123 | 35 | 3 | 53 | 153 | 1 | 123 | 1.03e-28 | 102 |
| MS.gene41086.t1 | MTR_1g057880 | 97.917 | 48 | 1 | 0 | 5 | 52 | 74 | 121 | 1.54e-26 | 97.4 |
| MS.gene41086.t1 | MTR_1g022010 | 61.194 | 67 | 18 | 2 | 5 | 65 | 165 | 229 | 2.44e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41086.t1 | AT3G02760 | 59.333 | 150 | 58 | 2 | 5 | 151 | 734 | 883 | 5.92e-51 | 174 |
| MS.gene41086.t1 | AT3G02760 | 59.333 | 150 | 58 | 2 | 5 | 151 | 734 | 883 | 5.92e-51 | 174 |
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAGAAAAAAAGAAAAT+AGG | + | 10676:42376-42395 | None:intergenic | 15.0% |
| !!! | AAAATAACTTGTGTTAAAAT+GGG | + | 10676:42194-42213 | None:intergenic | 15.0% |
| !! | AAAAACAGCAGATTATAAAA+AGG | + | 10676:42512-42531 | None:intergenic | 20.0% |
| !! | AAATATTCCTTGATTATTCT+GGG | - | 10676:42159-42178 | MS.gene41086:intron | 20.0% |
| !!! | AGTTGTTTCCTTATTAAATT+GGG | - | 10676:42440-42459 | MS.gene41086:intron | 20.0% |
| !!! | ATATTTTAGTGATGGATTAA+TGG | - | 10676:41931-41950 | MS.gene41086:CDS | 20.0% |
| !!! | GAAAATAACTTGTGTTAAAA+TGG | + | 10676:42195-42214 | None:intergenic | 20.0% |
| !!! | TAGTTGTTTCCTTATTAAAT+TGG | - | 10676:42439-42458 | MS.gene41086:intron | 20.0% |
| !!! | TTTTTTTCTTCTTTTTGTTG+TGG | - | 10676:42382-42401 | MS.gene41086:intron | 20.0% |
| ! | GAAATATTCCTTGATTATTC+TGG | - | 10676:42158-42177 | MS.gene41086:intron | 25.0% |
| ! | TGACACAATTATGTAATGTT+TGG | + | 10676:42222-42241 | None:intergenic | 25.0% |
| !! | AAAATTTTGTTGAGGAACTT+CGG | - | 10676:42802-42821 | MS.gene41086:intron | 25.0% |
| !!! | TTCTGCTTATATTTTAGTGA+TGG | - | 10676:41923-41942 | MS.gene41086:CDS | 25.0% |
| GTCTAGGCAATAATATAACA+AGG | + | 10676:42471-42490 | None:intergenic | 30.0% | |
| TAAATGAAACTGACAGAGAA+GGG | + | 10676:41884-41903 | None:intergenic | 30.0% | |
| TTCAATACCCAGAATAATCA+AGG | + | 10676:42169-42188 | None:intergenic | 30.0% | |
| ! | CCTCAACAAAATTTTCTCTA+GGG | + | 10676:42797-42816 | None:intergenic | 30.0% |
| ! | TCCTCAACAAAATTTTCTCT+AGG | + | 10676:42798-42817 | None:intergenic | 30.0% |
| ! | TGGATTAATGGTTTTTACAC+AGG | - | 10676:41943-41962 | MS.gene41086:CDS | 30.0% |
| ! | TTAATGGTTTTTACACAGGT+TGG | - | 10676:41947-41966 | MS.gene41086:CDS | 30.0% |
| !! | TTCTTTTTGTTGTGGTGTTT+TGG | - | 10676:42390-42409 | MS.gene41086:intron | 30.0% |
| !!! | AATCTGCTGTTTTTGTTAGA+TGG | - | 10676:42518-42537 | MS.gene41086:intron | 30.0% |
| AAATCCTTGATTCTTGAGAG+TGG | - | 10676:41822-41841 | MS.gene41086:CDS | 35.0% | |
| AACTGTTAGTTCATCTGTCT+AGG | + | 10676:42487-42506 | None:intergenic | 35.0% | |
| AAGTCCTAGTGAGCATATTA+GGG | - | 10676:42559-42578 | MS.gene41086:CDS | 35.0% | |
| AGGACGTTCCCAATTTAATA+AGG | + | 10676:42451-42470 | None:intergenic | 35.0% | |
| CAAAAGAATCAAGAATCCCT+TGG | - | 10676:42685-42704 | MS.gene41086:intron | 35.0% | |
| GTAAATGAAACTGACAGAGA+AGG | + | 10676:41885-41904 | None:intergenic | 35.0% | |
| TATCAAGCATTATGATCACC+TGG | + | 10676:42105-42124 | None:intergenic | 35.0% | |
| TATCTATCTGATGGAGGATA+TGG | + | 10676:42261-42280 | None:intergenic | 35.0% | |
| TGGAGTTAAAGCAGAATTCT+TGG | - | 10676:42629-42648 | MS.gene41086:CDS | 35.0% | |
| ! | CCCTAGAGAAAATTTTGTTG+AGG | - | 10676:42794-42813 | MS.gene41086:intron | 35.0% |
| ! | TTTGCATAGTCAAAGTGCTT+TGG | + | 10676:42669-42688 | None:intergenic | 35.0% |
| ATCCCTTGGATGATACTTGT+TGG | - | 10676:42699-42718 | MS.gene41086:intron | 40.0% | |
| ATGACTTGATTCATCACCAC+GGG | - | 10676:42310-42329 | MS.gene41086:intron | 40.0% | |
| CACCAACAAGTATCATCCAA+GGG | + | 10676:42704-42723 | None:intergenic | 40.0% | |
| CAGACAGTATAAGTAACAGC+AGG | + | 10676:42286-42305 | None:intergenic | 40.0% | |
| CATTCCCTAATATGCTCACT+AGG | + | 10676:42566-42585 | None:intergenic | 40.0% | |
| CTTCTGTTGTTGCTCCATTA+TGG | + | 10676:42074-42093 | None:intergenic | 40.0% | |
| GAAGTCCTAGTGAGCATATT+AGG | - | 10676:42558-42577 | MS.gene41086:CDS | 40.0% | |
| GATAACCTGATAGGGATGTT+TGG | - | 10676:41995-42014 | MS.gene41086:CDS | 40.0% | |
| GATGACTTGATTCATCACCA+CGG | - | 10676:42309-42328 | MS.gene41086:intron | 40.0% | |
| GCAGGAAATAAAAGAAGGCA+CGG | - | 10676:42725-42744 | MS.gene41086:intron | 40.0% | |
| GGGATGTATCTAATCACTAG+GGG | - | 10676:42331-42350 | MS.gene41086:intron | 40.0% | |
| GGGGATGTATCTAATCACTA+GGG | - | 10676:42330-42349 | MS.gene41086:intron | 40.0% | |
| GGTGAGCAGGAAATAAAAGA+AGG | - | 10676:42720-42739 | MS.gene41086:intron | 40.0% | |
| GTCGATACGATAACCTGATA+GGG | - | 10676:41987-42006 | MS.gene41086:CDS | 40.0% | |
| TCACCAACAAGTATCATCCA+AGG | + | 10676:42705-42724 | None:intergenic | 40.0% | |
| TCTGATGGAGGATATGGTTT+AGG | + | 10676:42255-42274 | None:intergenic | 40.0% | |
| TTGTTGAGGAACTTCGGAAA+CGG | - | 10676:42808-42827 | MS.gene41086:intron | 40.0% | |
| ! | CATAATGGAGCAACAACAGA+AGG | - | 10676:42072-42091 | MS.gene41086:CDS | 40.0% |
| ! | CTGCTGTTACTTATACTGTC+TGG | - | 10676:42284-42303 | MS.gene41086:intron | 40.0% |
| ! | TTACATCATTACCTGACTCG+AGG | + | 10676:42764-42783 | None:intergenic | 40.0% |
| !! | CGAGAGAGTATTTGCCATAA+TGG | - | 10676:42057-42076 | MS.gene41086:CDS | 40.0% |
| ACAACAGAAGGATCAGAACC+AGG | - | 10676:42084-42103 | MS.gene41086:CDS | 45.0% | |
| ACTAGGACTTCTGTCTTGGT+TGG | + | 10676:42549-42568 | None:intergenic | 45.0% | |
| AGTGATTAGATACATCCCCG+TGG | + | 10676:42329-42348 | None:intergenic | 45.0% | |
| CCAACCACTCTCAAGAATCA+AGG | + | 10676:41829-41848 | None:intergenic | 45.0% | |
| CCTTGATTCTTGAGAGTGGT+TGG | - | 10676:41826-41845 | MS.gene41086:CDS | 45.0% | |
| CGGGGATGTATCTAATCACT+AGG | - | 10676:42329-42348 | MS.gene41086:intron | 45.0% | |
| GGGAATGATGTAACACTAGC+TGG | - | 10676:42579-42598 | MS.gene41086:CDS | 45.0% | |
| GGTCGATACGATAACCTGAT+AGG | - | 10676:41986-42005 | MS.gene41086:CDS | 45.0% | |
| TCGAACCAAACATCCCTATC+AGG | + | 10676:42003-42022 | None:intergenic | 45.0% | |
| TGACTTGATTCATCACCACG+GGG | - | 10676:42311-42330 | MS.gene41086:intron | 45.0% | |
| ! | GATGATACTTGTTGGTGAGC+AGG | - | 10676:42707-42726 | MS.gene41086:intron | 45.0% |
| !! | CAGCAGGAGTATCTATCTGA+TGG | + | 10676:42270-42289 | None:intergenic | 45.0% |
| !! | CAGGAGTATCTATCTGATGG+AGG | + | 10676:42267-42286 | None:intergenic | 45.0% |
| !! | TCTTGAGAGTGGTTGGCTTA+TGG | - | 10676:41833-41852 | MS.gene41086:CDS | 45.0% |
| AGAGCTAGCTGGTGAACTGT+GGG | - | 10676:42602-42621 | MS.gene41086:CDS | 50.0% | |
| CAACTGAAAAGCCTCGAGTC+AGG | - | 10676:42750-42769 | MS.gene41086:intron | 50.0% | |
| GCTCACTAGGACTTCTGTCT+TGG | + | 10676:42553-42572 | None:intergenic | 50.0% | |
| TCGAAGCAAGTTCCAGCAGT+TGG | - | 10676:42019-42038 | MS.gene41086:CDS | 50.0% | |
| ! | CCAGCAGTTGGTGTAAGTCT+GGG | - | 10676:42031-42050 | MS.gene41086:CDS | 50.0% |
| ! | GTTGGTTCAATTGCTGCTGG+TGG | - | 10676:41965-41984 | MS.gene41086:CDS | 50.0% |
| ! | TCCAGCAGTTGGTGTAAGTC+TGG | - | 10676:42030-42049 | MS.gene41086:CDS | 50.0% |
| !! | CAGGTTGGTTCAATTGCTGC+TGG | - | 10676:41962-41981 | MS.gene41086:CDS | 50.0% |
| ACACTAGCTGGAGAGCTAGC+TGG | - | 10676:42591-42610 | MS.gene41086:CDS | 55.0% | |
| CCCAGACTTACACCAACTGC+TGG | + | 10676:42034-42053 | None:intergenic | 55.0% | |
| GAGAGCTAGCTGGTGAACTG+TGG | - | 10676:42601-42620 | MS.gene41086:CDS | 55.0% | |
| GCTGGTGAACTGTGGGATGC+TGG | - | 10676:42609-42628 | MS.gene41086:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10676 | gene | 41799 | 42842 | 41799 | ID=MS.gene41086 |
| 10676 | mRNA | 41799 | 42842 | 41799 | ID=MS.gene41086.t1;Parent=MS.gene41086 |
| 10676 | exon | 42828 | 42842 | 42828 | ID=MS.gene41086.t1.exon1;Parent=MS.gene41086.t1 |
| 10676 | CDS | 42828 | 42842 | 42828 | ID=cds.MS.gene41086.t1;Parent=MS.gene41086.t1 |
| 10676 | exon | 42536 | 42676 | 42536 | ID=MS.gene41086.t1.exon2;Parent=MS.gene41086.t1 |
| 10676 | CDS | 42536 | 42676 | 42536 | ID=cds.MS.gene41086.t1;Parent=MS.gene41086.t1 |
| 10676 | exon | 41799 | 42104 | 41799 | ID=MS.gene41086.t1.exon3;Parent=MS.gene41086.t1 |
| 10676 | CDS | 41799 | 42104 | 41799 | ID=cds.MS.gene41086.t1;Parent=MS.gene41086.t1 |
| Gene Sequence |
| Protein sequence |
