Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45553.t1 | XP_003597439.1 | 79.9 | 448 | 86 | 3 | 1 | 447 | 1 | 445 | 8.20E-176 | 626.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45553.t1 | G7IUC5 | 79.9 | 448 | 86 | 3 | 1 | 447 | 1 | 445 | 5.9e-176 | 626.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene45553.t1 | TF | Trihelix |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene45553 | 0.838551 | 2.69E-57 | -1.69E-46 |
| MS.gene049254 | MS.gene45553 | 0.816805 | 4.58E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene45553 | 0.818954 | 1.50E-52 | -1.69E-46 |
| MS.gene049452 | MS.gene45553 | 0.840847 | 6.80E-58 | -1.69E-46 |
| MS.gene049983 | MS.gene45553 | 0.818702 | 1.71E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene45553 | 0.825316 | 5.01E-54 | -1.69E-46 |
| MS.gene050355 | MS.gene45553 | 0.801706 | 7.93E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene45553 | 0.805932 | 1.05E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene45553 | 0.803027 | 4.24E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene45553 | 0.826516 | 2.60E-54 | -1.69E-46 |
| MS.gene050962 | MS.gene45553 | 0.817326 | 3.49E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene45553 | 0.822632 | 2.14E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene45553 | 0.82852 | 8.59E-55 | -1.69E-46 |
| MS.gene051328 | MS.gene45553 | 0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene45553 | 0.818813 | 1.61E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene45553 | 0.806041 | 9.97E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene45553 | 0.817604 | 3.03E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene45553 | 0.837053 | 6.52E-57 | -1.69E-46 |
| MS.gene052582 | MS.gene45553 | 0.825908 | 3.63E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene45553 | 0.815858 | 7.45E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene45553 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene45553 | 0.808031 | 3.78E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene45553 | 0.847254 | 1.30E-59 | -1.69E-46 |
| MS.gene053008 | MS.gene45553 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene45553 | 0.828364 | 9.37E-55 | -1.69E-46 |
| MS.gene053931 | MS.gene45553 | 0.830616 | 2.66E-55 | -1.69E-46 |
| MS.gene05414 | MS.gene45553 | 0.816004 | 6.91E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene45553 | 0.827826 | 1.26E-54 | -1.69E-46 |
| MS.gene05423 | MS.gene45553 | 0.802453 | 5.57E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene45553 | 0.825769 | 3.91E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene45553 | 0.864261 | 1.38E-64 | -1.69E-46 |
| MS.gene054775 | MS.gene45553 | 0.814484 | 1.51E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene45553 | 0.831117 | 2.00E-55 | -1.69E-46 |
| MS.gene055222 | MS.gene45553 | 0.826657 | 2.40E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene45553 | 0.801797 | 7.60E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene45553 | 0.814425 | 1.55E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene45553 | 0.808907 | 2.46E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene45553 | 0.819561 | 1.09E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene45553 | 0.824213 | 9.11E-54 | -1.69E-46 |
| MS.gene056162 | MS.gene45553 | 0.804971 | 1.67E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene45553 | 0.829585 | 4.74E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene45553 | 0.806261 | 8.96E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene45553 | 0.818173 | 2.25E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene45553 | 0.828601 | 8.21E-55 | -1.69E-46 |
| MS.gene05690 | MS.gene45553 | 0.827021 | 1.97E-54 | -1.69E-46 |
| MS.gene057034 | MS.gene45553 | 0.801373 | 9.28E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene45553 | 0.827441 | 1.56E-54 | -1.69E-46 |
| MS.gene057444 | MS.gene45553 | 0.809734 | 1.63E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene45553 | 0.823594 | 1.27E-53 | -1.69E-46 |
| MS.gene058008 | MS.gene45553 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene45553 | 0.831039 | 2.09E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene45553 | 0.818803 | 1.62E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene45553 | 0.812954 | 3.27E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene45553 | 0.815438 | 9.24E-52 | -1.69E-46 |
| MS.gene058781 | MS.gene45553 | 0.810319 | 1.22E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene45553 | 0.835912 | 1.27E-56 | -1.69E-46 |
| MS.gene058979 | MS.gene45553 | 0.835135 | 2.00E-56 | -1.69E-46 |
| MS.gene05954 | MS.gene45553 | 0.807521 | 4.85E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene45553 | 0.820186 | 7.83E-53 | -1.69E-46 |
| MS.gene05979 | MS.gene45553 | 0.802051 | 6.74E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene45553 | 0.851198 | 1.04E-60 | -1.69E-46 |
| MS.gene060011 | MS.gene45553 | 0.824478 | 7.90E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene45553 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene45553 | 0.827411 | 1.59E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene45553 | 0.832259 | 1.05E-55 | -1.69E-46 |
| MS.gene060802 | MS.gene45553 | 0.812149 | 4.90E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene45553 | 0.808317 | 3.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45553.t1 | MTR_2g098080 | 95.982 | 448 | 14 | 3 | 1 | 447 | 1 | 445 | 0.0 | 882 |
| MS.gene45553.t1 | MTR_1g098920 | 47.500 | 360 | 162 | 8 | 84 | 418 | 92 | 449 | 2.46e-95 | 294 |
| MS.gene45553.t1 | MTR_6g035315 | 46.450 | 338 | 154 | 4 | 102 | 413 | 105 | 441 | 3.08e-82 | 263 |
| MS.gene45553.t1 | MTR_7g103390 | 47.083 | 240 | 113 | 5 | 4 | 242 | 3 | 229 | 1.20e-60 | 204 |
| MS.gene45553.t1 | MTR_1g061640 | 60.248 | 161 | 58 | 3 | 83 | 242 | 94 | 249 | 1.34e-55 | 192 |
| MS.gene45553.t1 | MTR_1g061640 | 41.441 | 111 | 61 | 1 | 309 | 415 | 371 | 481 | 3.29e-23 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45553.t1 | AT1G21200 | 39.787 | 470 | 204 | 12 | 1 | 418 | 1 | 443 | 6.41e-89 | 278 |
| MS.gene45553.t1 | AT1G21200 | 39.787 | 470 | 204 | 12 | 1 | 418 | 1 | 443 | 6.41e-89 | 278 |
| MS.gene45553.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 83 | 418 | 60 | 385 | 1.10e-71 | 231 |
| MS.gene45553.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 83 | 418 | 60 | 385 | 1.10e-71 | 231 |
| MS.gene45553.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 83 | 418 | 60 | 385 | 1.10e-71 | 231 |
| MS.gene45553.t1 | AT3G10040 | 37.634 | 372 | 188 | 6 | 85 | 429 | 74 | 428 | 4.21e-66 | 218 |
Find 115 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAATAATAATAATCAAAT+TGG | 0.228579 | 2.1:+6192556 | MS.gene45553:CDS |
| TTCTCCCACGGTGACTTTGC+AGG | 0.254875 | 2.1:-6193420 | None:intergenic |
| TCAATTATGAATGGTTCTTC+TGG | 0.281471 | 2.1:+6192403 | None:intergenic |
| GGTTCTTCTGGTTTGGGTGG+TGG | 0.300179 | 2.1:+6192415 | MS.gene45553:CDS |
| TATGAATGGTTCTTCTGGTT+TGG | 0.302937 | 2.1:+6192408 | None:intergenic |
| CAAGTCAAGATCATGACTAT+TGG | 0.311830 | 2.1:-6193148 | None:intergenic |
| ATGAATGGTTCTTCTGGTTT+GGG | 0.316755 | 2.1:+6192409 | MS.gene45553:CDS |
| GTTCTTCTGGTTTGGGTGGT+GGG | 0.328898 | 2.1:+6192416 | MS.gene45553:CDS |
| ACCAATGGAGGTAGGGTCTA+AGG | 0.337698 | 2.1:-6193679 | None:intergenic |
| GTGAGGAGGATGGCCACTTT+TGG | 0.369260 | 2.1:+6193340 | MS.gene45553:CDS |
| GGAGAAAAGAGAGTGGATTA+AGG | 0.381772 | 2.1:+6193437 | MS.gene45553:CDS |
| TATGAATATGATAACTGGTT+TGG | 0.383314 | 2.1:+6192528 | MS.gene45553:CDS |
| GGATAGGGAGCTGGAGAAGC+TGG | 0.386271 | 2.1:+6193560 | MS.gene45553:CDS |
| CAACATATGAATATGATAAC+TGG | 0.410282 | 2.1:+6192523 | MS.gene45553:CDS |
| TCCTGCATTGATGGATTCAA+TGG | 0.411710 | 2.1:+6193023 | MS.gene45553:CDS |
| GAGGATGATGAGTTGGATAA+TGG | 0.423042 | 2.1:+6193252 | MS.gene45553:CDS |
| AAGTCAAGATCATGACTATT+GGG | 0.423237 | 2.1:-6193147 | None:intergenic |
| GAAGTTCAAAAGCCCTGGCT+TGG | 0.432989 | 2.1:-6193497 | None:intergenic |
| GAGCATGGTTACGGAGAAGA+TGG | 0.435266 | 2.1:+6192670 | MS.gene45553:CDS |
| GATGGTGTTAAGAAGAAATC+CGG | 0.439201 | 2.1:+6192832 | MS.gene45553:CDS |
| TCTCCCACGGTGACTTTGCA+GGG | 0.439902 | 2.1:-6193419 | None:intergenic |
| ATCCGGGGTGTCACAGAAGA+AGG | 0.448557 | 2.1:+6192849 | MS.gene45553:CDS |
| TGGTAGGGGAACTTGTTGTC+AGG | 0.449451 | 2.1:+6192990 | MS.gene45553:CDS |
| GAAGACGATGAGCATGGTTA+CGG | 0.454149 | 2.1:+6192661 | MS.gene45553:CDS |
| GTGAGTTGTGTTGGTGATGA+TGG | 0.455421 | 2.1:+6192793 | MS.gene45553:CDS |
| GAAGAAACTTGAGAGTGAAA+TGG | 0.458951 | 2.1:+6193380 | MS.gene45553:CDS |
| GGTTGAGAATCCTGCATTGA+TGG | 0.463782 | 2.1:+6193014 | MS.gene45553:CDS |
| GATACCGCAGCAAGAAGGAT+AGG | 0.473004 | 2.1:+6193544 | MS.gene45553:CDS |
| TGACTTGCATAGTTATTCGT+TGG | 0.479428 | 2.1:+6193164 | MS.gene45553:CDS |
| TTCAGAAACAGCGGTTTAAG+TGG | 0.489001 | 2.1:+6193517 | MS.gene45553:CDS |
| ACCATTGAATCCATCAATGC+AGG | 0.492133 | 2.1:-6193024 | None:intergenic |
| AATATTAATGCACGTGGTGA+TGG | 0.497668 | 2.1:+6193279 | MS.gene45553:CDS |
| TGTTGTTGATGCTTATGAAA+TGG | 0.499582 | 2.1:-6192481 | None:intergenic |
| GGTGATGGAGGGAGAATGGA+AGG | 0.501886 | 2.1:+6193294 | MS.gene45553:CDS |
| TCTAAGATAATGATAAGTAA+AGG | 0.502250 | 2.1:+6192889 | MS.gene45553:CDS |
| AATTGTGTTTCAATTATGAA+TGG | 0.507262 | 2.1:+6192394 | None:intergenic |
| AATGGTTAACTTGTCTGCTA+AGG | 0.514836 | 2.1:+6193041 | MS.gene45553:CDS |
| TTGTGGCCTCTTGATACCAA+TGG | 0.514883 | 2.1:-6193694 | None:intergenic |
| AATGGTTCTTCTGGTTTGGG+TGG | 0.516998 | 2.1:+6192412 | MS.gene45553:CDS |
| TCCTTAGACCCTACCTCCAT+TGG | 0.518261 | 2.1:+6193678 | MS.gene45553:CDS |
| GGTTGAATGAGATGCTTGGT+AGG | 0.519981 | 2.1:+6192974 | MS.gene45553:CDS |
| CATAGTTATTCGTTGGAACA+CGG | 0.524019 | 2.1:+6193171 | MS.gene45553:CDS |
| AAAATGTTGACTTCCAAGCC+AGG | 0.526284 | 2.1:+6193484 | MS.gene45553:CDS |
| CACTAGACTGCGGCCAAAAG+TGG | 0.527780 | 2.1:-6193353 | None:intergenic |
| TTTCTGAAGTTCAAAAGCCC+TGG | 0.531742 | 2.1:-6193502 | None:intergenic |
| GGTGATGATGGAACAATTAG+TGG | 0.532799 | 2.1:+6192805 | MS.gene45553:CDS |
| TGCAATTTGAAAATGAGCAC+AGG | 0.532921 | 2.1:+6193601 | MS.gene45553:CDS |
| CAGCAAGAAGGATAGGGAGC+TGG | 0.539921 | 2.1:+6193551 | MS.gene45553:CDS |
| TGGGTATCCTTTGACCATTG+TGG | 0.541818 | 2.1:-6193128 | None:intergenic |
| ATTAATGCACGTGGTGATGG+AGG | 0.542789 | 2.1:+6193282 | MS.gene45553:CDS |
| TAACTTGTCTGCTAAGGCTA+AGG | 0.548373 | 2.1:+6193047 | MS.gene45553:CDS |
| TGAAGATAACAATGAAAGTG+AGG | 0.549731 | 2.1:+6193233 | MS.gene45553:CDS |
| ATAGCAGTGGTGAGTTGTGT+TGG | 0.551265 | 2.1:+6192784 | MS.gene45553:CDS |
| TAGGGGAACTTGTTGTCAGG+TGG | 0.556404 | 2.1:+6192993 | MS.gene45553:CDS |
| TGAAAGTGAGGATGATGAGT+TGG | 0.557152 | 2.1:+6193245 | MS.gene45553:CDS |
| AACTTGAGAGTGAAATGGCA+AGG | 0.557312 | 2.1:+6193385 | MS.gene45553:CDS |
| GTTGAATGAGATGCTTGGTA+GGG | 0.559093 | 2.1:+6192975 | MS.gene45553:CDS |
| TATCAAGAGGCCACAAGGGA+GGG | 0.560647 | 2.1:+6193701 | MS.gene45553:CDS |
| GCTTATGAAATGGAGATTCA+AGG | 0.561619 | 2.1:-6192471 | None:intergenic |
| ATACCGCAGCAAGAAGGATA+GGG | 0.564239 | 2.1:+6193545 | MS.gene45553:CDS |
| AAAGCTGAAGCAGAGAGAGC+AGG | 0.565355 | 2.1:+6193629 | MS.gene45553:CDS |
| TTGATATGTTCCCTCCCTTG+TGG | 0.565958 | 2.1:-6193711 | None:intergenic |
| GTCCACTTCATTCTCTGCCA+AGG | 0.566514 | 2.1:-6192739 | None:intergenic |
| ACTTGAATAAGAGATATAAG+AGG | 0.568508 | 2.1:+6192953 | MS.gene45553:CDS |
| TGTCACAGAAGAAGGGTAAG+TGG | 0.569426 | 2.1:+6192857 | MS.gene45553:CDS |
| TCTTGATACCAATGGAGGTA+GGG | 0.573392 | 2.1:-6193686 | None:intergenic |
| GGAGCTGGAGAAGCTGGCGA+TGG | 0.574129 | 2.1:+6193566 | MS.gene45553:CDS |
| AAGAGGTTGAATGAGATGCT+TGG | 0.576189 | 2.1:+6192970 | MS.gene45553:CDS |
| AGCAGGAGGCAGAGTTCAGT+AGG | 0.578594 | 2.1:+6193646 | MS.gene45553:CDS |
| CAACAGACTCAGTTAGCTCA+TGG | 0.578956 | 2.1:+6192499 | MS.gene45553:CDS |
| ATGGTGTTAAGAAGAAATCC+GGG | 0.579854 | 2.1:+6192833 | MS.gene45553:CDS |
| CTCTTGATACCAATGGAGGT+AGG | 0.581041 | 2.1:-6193687 | None:intergenic |
| TTGAATGAGATGCTTGGTAG+GGG | 0.587060 | 2.1:+6192976 | MS.gene45553:CDS |
| TAGAAATAAATTAAGTGAGG+AGG | 0.587853 | 2.1:+6193326 | MS.gene45553:CDS |
| ACGTGGTGATGGAGGGAGAA+TGG | 0.588300 | 2.1:+6193290 | MS.gene45553:CDS |
| ACCCTTCTTCTGTGACACCC+CGG | 0.588573 | 2.1:-6192851 | None:intergenic |
| TGATAGAAATAAATTAAGTG+AGG | 0.588915 | 2.1:+6193323 | MS.gene45553:CDS |
| GTTAAGATACCGCAGCAAGA+AGG | 0.589509 | 2.1:+6193539 | MS.gene45553:CDS |
| TTAATGCACGTGGTGATGGA+GGG | 0.592885 | 2.1:+6193283 | MS.gene45553:CDS |
| AAATGTTGACTTCCAAGCCA+GGG | 0.593930 | 2.1:+6193485 | MS.gene45553:CDS |
| ATAGTTATTCGTTGGAACAC+GGG | 0.600371 | 2.1:+6193172 | MS.gene45553:CDS |
| TTATTCGTTGGAACACGGGA+AGG | 0.601197 | 2.1:+6193176 | MS.gene45553:CDS |
| GTGCATACCACAATGGTCAA+AGG | 0.603169 | 2.1:+6193121 | MS.gene45553:CDS |
| TGATGGAACAATTAGTGGTG+TGG | 0.604354 | 2.1:+6192810 | MS.gene45553:CDS |
| ACTTGAGAGTGAAATGGCAA+GGG | 0.606060 | 2.1:+6193386 | MS.gene45553:CDS |
| AGTGGACGGATAATGTGGTT+GGG | 0.610749 | 2.1:+6192755 | MS.gene45553:CDS |
| AAGTGGACGGATAATGTGGT+TGG | 0.613391 | 2.1:+6192754 | MS.gene45553:CDS |
| GTATCAAGAGGCCACAAGGG+AGG | 0.615865 | 2.1:+6193700 | MS.gene45553:CDS |
| AACTTATCCTCACACTGCTG+CGG | 0.618536 | 2.1:-6192925 | None:intergenic |
| CTACCTCCATTGGTATCAAG+AGG | 0.620308 | 2.1:+6193688 | MS.gene45553:CDS |
| AGCATGGTTACGGAGAAGAT+GGG | 0.623013 | 2.1:+6192671 | MS.gene45553:CDS |
| GGAACAATTAGTGGTGTGGA+TGG | 0.625621 | 2.1:+6192814 | MS.gene45553:CDS |
| AATAAATTAAGTGAGGAGGA+TGG | 0.626359 | 2.1:+6193330 | MS.gene45553:CDS |
| AATGAAGTGGACGGATAATG+TGG | 0.631463 | 2.1:+6192750 | MS.gene45553:CDS |
| AGGGAGGGAACATATCAACT+TGG | 0.631664 | 2.1:+6193716 | MS.gene45553:CDS |
| CTCCTTGGCAGAGAATGAAG+TGG | 0.631774 | 2.1:+6192737 | MS.gene45553:CDS |
| TGTGTCGCCGCAGCAGTGTG+AGG | 0.632288 | 2.1:+6192918 | MS.gene45553:CDS |
| GCTCCCTATCCTTCTTGCTG+CGG | 0.632768 | 2.1:-6193548 | None:intergenic |
| GGTTGGGCTTCTTATAGCAG+TGG | 0.632989 | 2.1:+6192771 | MS.gene45553:CDS |
| ATTGGTATCAAGAGGCCACA+AGG | 0.633070 | 2.1:+6193696 | MS.gene45553:CDS |
| TTCTTCATCCCACTAGACTG+CGG | 0.642211 | 2.1:-6193363 | None:intergenic |
| GCTGAAGCAGAGAGAGCAGG+AGG | 0.642379 | 2.1:+6193632 | MS.gene45553:CDS |
| ATGAGCGAAGACGATGAGCA+TGG | 0.644222 | 2.1:+6192655 | MS.gene45553:CDS |
| TTGGCAGAGAATGAAGTGGA+CGG | 0.645694 | 2.1:+6192741 | MS.gene45553:CDS |
| CACCGTGGGAGAAAAGAGAG+TGG | 0.647688 | 2.1:+6193430 | MS.gene45553:CDS |
| TGGCCTCTTGATACCAATGG+AGG | 0.649145 | 2.1:-6193691 | None:intergenic |
| GTGATGGAGGGAGAATGGAA+GGG | 0.653559 | 2.1:+6193295 | MS.gene45553:CDS |
| TCCGGGGTGTCACAGAAGAA+GGG | 0.653871 | 2.1:+6192850 | MS.gene45553:CDS |
| AGACCCTGCAAAGTCACCGT+GGG | 0.657508 | 2.1:+6193416 | MS.gene45553:CDS |
| TTGGTATCAAGAGGCCACAA+GGG | 0.674612 | 2.1:+6193697 | MS.gene45553:CDS |
| GAAATGTGTGCATACCACAA+TGG | 0.676978 | 2.1:+6193114 | MS.gene45553:CDS |
| GGAATTAATATTAATGCACG+TGG | 0.680730 | 2.1:+6193273 | MS.gene45553:CDS |
| AAGACCCTGCAAAGTCACCG+TGG | 0.699063 | 2.1:+6193415 | MS.gene45553:CDS |
| TGGTGTTAAGAAGAAATCCG+GGG | 0.737936 | 2.1:+6192834 | MS.gene45553:CDS |
| AAGGACTCAAGAGATCACGA+TGG | 0.786747 | 2.1:+6193195 | MS.gene45553:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATAATAATAATCAAAT+TGG | + | chr2.1:6192556-6192575 | MS.gene45553:CDS | 10.0% |
| !!! | AAAAATTTGAATTTTGGTAA+AGG | + | chr2.1:6192592-6192611 | MS.gene45553:CDS | 15.0% |
| !! | TGATAGAAATAAATTAAGTG+AGG | + | chr2.1:6193323-6193342 | MS.gene45553:CDS | 20.0% |
| !!! | GAAGTGAAAAATTTGAATTT+TGG | + | chr2.1:6192586-6192605 | MS.gene45553:CDS | 20.0% |
| !!! | TCTAAGATAATGATAAGTAA+AGG | + | chr2.1:6192889-6192908 | MS.gene45553:CDS | 20.0% |
| ! | ACTTGAATAAGAGATATAAG+AGG | + | chr2.1:6192953-6192972 | MS.gene45553:CDS | 25.0% |
| ! | CAACATATGAATATGATAAC+TGG | + | chr2.1:6192523-6192542 | MS.gene45553:CDS | 25.0% |
| ! | TAGAAATAAATTAAGTGAGG+AGG | + | chr2.1:6193326-6193345 | MS.gene45553:CDS | 25.0% |
| ! | TATGAATATGATAACTGGTT+TGG | + | chr2.1:6192528-6192547 | MS.gene45553:CDS | 25.0% |
| !!! | TTGAATTTTGGTAAAGGAAA+GGG | + | chr2.1:6192598-6192617 | MS.gene45553:CDS | 25.0% |
| !!! | TTTGAATTTTGGTAAAGGAA+AGG | + | chr2.1:6192597-6192616 | MS.gene45553:CDS | 25.0% |
| AAGTCAAGATCATGACTATT+GGG | - | chr2.1:6193150-6193169 | None:intergenic | 30.0% | |
| AATAAATTAAGTGAGGAGGA+TGG | + | chr2.1:6193330-6193349 | MS.gene45553:CDS | 30.0% | |
| GGAATTAATATTAATGCACG+TGG | + | chr2.1:6193273-6193292 | MS.gene45553:CDS | 30.0% | |
| TGAAGATAACAATGAAAGTG+AGG | + | chr2.1:6193233-6193252 | MS.gene45553:CDS | 30.0% | |
| TGTTGTTGATGCTTATGAAA+TGG | - | chr2.1:6192484-6192503 | None:intergenic | 30.0% | |
| !!! | AATTGCGAGAATTTTTTCGA+TGG | + | chr2.1:6192694-6192713 | MS.gene45553:CDS | 30.0% |
| AATATTAATGCACGTGGTGA+TGG | + | chr2.1:6193279-6193298 | MS.gene45553:CDS | 35.0% | |
| ATAGTTATTCGTTGGAACAC+GGG | + | chr2.1:6193172-6193191 | MS.gene45553:CDS | 35.0% | |
| CAAGTCAAGATCATGACTAT+TGG | - | chr2.1:6193151-6193170 | None:intergenic | 35.0% | |
| CATAGTTATTCGTTGGAACA+CGG | + | chr2.1:6193171-6193190 | MS.gene45553:CDS | 35.0% | |
| GAAGAAACTTGAGAGTGAAA+TGG | + | chr2.1:6193380-6193399 | MS.gene45553:CDS | 35.0% | |
| GCTTATGAAATGGAGATTCA+AGG | - | chr2.1:6192474-6192493 | None:intergenic | 35.0% | |
| TGCAATTTGAAAATGAGCAC+AGG | + | chr2.1:6193601-6193620 | MS.gene45553:CDS | 35.0% | |
| ! | ATGGTGTTAAGAAGAAATCC+GGG | + | chr2.1:6192833-6192852 | MS.gene45553:CDS | 35.0% |
| ! | CTTTTGAACTTCAGAAACAG+CGG | + | chr2.1:6193508-6193527 | MS.gene45553:CDS | 35.0% |
| ! | GATGGTGTTAAGAAGAAATC+CGG | + | chr2.1:6192832-6192851 | MS.gene45553:CDS | 35.0% |
| ! | TGACTTGCATAGTTATTCGT+TGG | + | chr2.1:6193164-6193183 | MS.gene45553:CDS | 35.0% |
| !! | AATGGTTAACTTGTCTGCTA+AGG | + | chr2.1:6193041-6193060 | MS.gene45553:CDS | 35.0% |
| !! | ATGAATGGTTCTTCTGGTTT+GGG | + | chr2.1:6192409-6192428 | MS.gene45553:CDS | 35.0% |
| !!! | AATTTTTTCGATGGTGGGAA+GGG | + | chr2.1:6192703-6192722 | MS.gene45553:CDS | 35.0% |
| AACTTGAGAGTGAAATGGCA+AGG | + | chr2.1:6193385-6193404 | MS.gene45553:CDS | 40.0% | |
| AAGAGGTTGAATGAGATGCT+TGG | + | chr2.1:6192970-6192989 | MS.gene45553:CDS | 40.0% | |
| AATGAAGTGGACGGATAATG+TGG | + | chr2.1:6192750-6192769 | MS.gene45553:CDS | 40.0% | |
| ACCATTGAATCCATCAATGC+AGG | - | chr2.1:6193027-6193046 | None:intergenic | 40.0% | |
| ACTTGAGAGTGAAATGGCAA+GGG | + | chr2.1:6193386-6193405 | MS.gene45553:CDS | 40.0% | |
| GAAATGTGTGCATACCACAA+TGG | + | chr2.1:6193114-6193133 | MS.gene45553:CDS | 40.0% | |
| GTTGAATGAGATGCTTGGTA+GGG | + | chr2.1:6192975-6192994 | MS.gene45553:CDS | 40.0% | |
| TCTTGATACCAATGGAGGTA+GGG | - | chr2.1:6193689-6193708 | None:intergenic | 40.0% | |
| TGAAAGTGAGGATGATGAGT+TGG | + | chr2.1:6193245-6193264 | MS.gene45553:CDS | 40.0% | |
| TGATGGAACAATTAGTGGTG+TGG | + | chr2.1:6192810-6192829 | MS.gene45553:CDS | 40.0% | |
| TTCAGAAACAGCGGTTTAAG+TGG | + | chr2.1:6193517-6193536 | MS.gene45553:CDS | 40.0% | |
| TTGAATGAGATGCTTGGTAG+GGG | + | chr2.1:6192976-6192995 | MS.gene45553:CDS | 40.0% | |
| ! | GAGGATGATGAGTTGGATAA+TGG | + | chr2.1:6193252-6193271 | MS.gene45553:CDS | 40.0% |
| ! | GGAAGTCAACATTTTGCTCT+TGG | - | chr2.1:6193479-6193498 | None:intergenic | 40.0% |
| ! | TAACTTGTCTGCTAAGGCTA+AGG | + | chr2.1:6193047-6193066 | MS.gene45553:CDS | 40.0% |
| ! | TGGTGTTAAGAAGAAATCCG+GGG | + | chr2.1:6192834-6192853 | MS.gene45553:CDS | 40.0% |
| !! | AAAATGTTGACTTCCAAGCC+AGG | + | chr2.1:6193484-6193503 | MS.gene45553:CDS | 40.0% |
| !! | AAATGTTGACTTCCAAGCCA+GGG | + | chr2.1:6193485-6193504 | MS.gene45553:CDS | 40.0% |
| !! | GGAGAAAAGAGAGTGGATTA+AGG | + | chr2.1:6193437-6193456 | MS.gene45553:CDS | 40.0% |
| !! | GGTGATGATGGAACAATTAG+TGG | + | chr2.1:6192805-6192824 | MS.gene45553:CDS | 40.0% |
| !! | TCCTGCATTGATGGATTCAA+TGG | + | chr2.1:6193023-6193042 | MS.gene45553:CDS | 40.0% |
| !! | TTTCTGAAGTTCAAAAGCCC+TGG | - | chr2.1:6193505-6193524 | None:intergenic | 40.0% |
| !!! | ATTTTTTCGATGGTGGGAAG+GGG | + | chr2.1:6192704-6192723 | MS.gene45553:CDS | 40.0% |
| !!! | GAATTTTTTCGATGGTGGGA+AGG | + | chr2.1:6192702-6192721 | MS.gene45553:CDS | 40.0% |
| !!! | GCGAGAATTTTTTCGATGGT+GGG | + | chr2.1:6192698-6192717 | MS.gene45553:CDS | 40.0% |
| !!! | TGCGAGAATTTTTTCGATGG+TGG | + | chr2.1:6192697-6192716 | MS.gene45553:CDS | 40.0% |
| AACTTATCCTCACACTGCTG+CGG | - | chr2.1:6192928-6192947 | None:intergenic | 45.0% | |
| AAGGACTCAAGAGATCACGA+TGG | + | chr2.1:6193195-6193214 | MS.gene45553:CDS | 45.0% | |
| AAGTGGACGGATAATGTGGT+TGG | + | chr2.1:6192754-6192773 | MS.gene45553:CDS | 45.0% | |
| AGCATGGTTACGGAGAAGAT+GGG | + | chr2.1:6192671-6192690 | MS.gene45553:CDS | 45.0% | |
| AGGGAGGGAACATATCAACT+TGG | + | chr2.1:6193716-6193735 | MS.gene45553:CDS | 45.0% | |
| AGTGGACGGATAATGTGGTT+GGG | + | chr2.1:6192755-6192774 | MS.gene45553:CDS | 45.0% | |
| ATACCGCAGCAAGAAGGATA+GGG | + | chr2.1:6193545-6193564 | MS.gene45553:CDS | 45.0% | |
| ATAGCAGTGGTGAGTTGTGT+TGG | + | chr2.1:6192784-6192803 | MS.gene45553:CDS | 45.0% | |
| ATTGGTATCAAGAGGCCACA+AGG | + | chr2.1:6193696-6193715 | MS.gene45553:CDS | 45.0% | |
| CAACAGACTCAGTTAGCTCA+TGG | + | chr2.1:6192499-6192518 | MS.gene45553:CDS | 45.0% | |
| CTACCTCCATTGGTATCAAG+AGG | + | chr2.1:6193688-6193707 | MS.gene45553:CDS | 45.0% | |
| CTCTTGATACCAATGGAGGT+AGG | - | chr2.1:6193690-6193709 | None:intergenic | 45.0% | |
| GAAGACGATGAGCATGGTTA+CGG | + | chr2.1:6192661-6192680 | MS.gene45553:CDS | 45.0% | |
| GGAACAATTAGTGGTGTGGA+TGG | + | chr2.1:6192814-6192833 | MS.gene45553:CDS | 45.0% | |
| GGTTGAATGAGATGCTTGGT+AGG | + | chr2.1:6192974-6192993 | MS.gene45553:CDS | 45.0% | |
| GGTTGAGAATCCTGCATTGA+TGG | + | chr2.1:6193014-6193033 | MS.gene45553:CDS | 45.0% | |
| GTGCATACCACAATGGTCAA+AGG | + | chr2.1:6193121-6193140 | MS.gene45553:CDS | 45.0% | |
| GTTAAGATACCGCAGCAAGA+AGG | + | chr2.1:6193539-6193558 | MS.gene45553:CDS | 45.0% | |
| TGGGTATCCTTTGACCATTG+TGG | - | chr2.1:6193131-6193150 | None:intergenic | 45.0% | |
| TGTCACAGAAGAAGGGTAAG+TGG | + | chr2.1:6192857-6192876 | MS.gene45553:CDS | 45.0% | |
| TTATTCGTTGGAACACGGGA+AGG | + | chr2.1:6193176-6193195 | MS.gene45553:CDS | 45.0% | |
| TTCTTCATCCCACTAGACTG+CGG | - | chr2.1:6193366-6193385 | None:intergenic | 45.0% | |
| TTGATATGTTCCCTCCCTTG+TGG | - | chr2.1:6193714-6193733 | None:intergenic | 45.0% | |
| TTGGCAGAGAATGAAGTGGA+CGG | + | chr2.1:6192741-6192760 | MS.gene45553:CDS | 45.0% | |
| TTGGTATCAAGAGGCCACAA+GGG | + | chr2.1:6193697-6193716 | MS.gene45553:CDS | 45.0% | |
| TTGTGGCCTCTTGATACCAA+TGG | - | chr2.1:6193697-6193716 | None:intergenic | 45.0% | |
| ! | ATCCACTCTCTTTTCTCCCA+CGG | - | chr2.1:6193435-6193454 | None:intergenic | 45.0% |
| ! | ATGGTGGGAAGGGGAAAAAA+GGG | + | chr2.1:6192713-6192732 | MS.gene45553:CDS | 45.0% |
| !! | AATGGTTCTTCTGGTTTGGG+TGG | + | chr2.1:6192412-6192431 | MS.gene45553:CDS | 45.0% |
| !! | ATTAATGCACGTGGTGATGG+AGG | + | chr2.1:6193282-6193301 | MS.gene45553:CDS | 45.0% |
| !! | GTGAGTTGTGTTGGTGATGA+TGG | + | chr2.1:6192793-6192812 | MS.gene45553:CDS | 45.0% |
| !! | TTAATGCACGTGGTGATGGA+GGG | + | chr2.1:6193283-6193302 | MS.gene45553:CDS | 45.0% |
| AAAGCTGAAGCAGAGAGAGC+AGG | + | chr2.1:6193629-6193648 | MS.gene45553:CDS | 50.0% | |
| ACCAATGGAGGTAGGGTCTA+AGG | - | chr2.1:6193682-6193701 | None:intergenic | 50.0% | |
| AGGGGAAAAAAGGGTCTCCT+TGG | + | chr2.1:6192722-6192741 | MS.gene45553:CDS | 50.0% | |
| ATGAGCGAAGACGATGAGCA+TGG | + | chr2.1:6192655-6192674 | MS.gene45553:CDS | 50.0% | |
| CTCCTTGGCAGAGAATGAAG+TGG | + | chr2.1:6192737-6192756 | MS.gene45553:CDS | 50.0% | |
| GAAGTTCAAAAGCCCTGGCT+TGG | - | chr2.1:6193500-6193519 | None:intergenic | 50.0% | |
| GAGCATGGTTACGGAGAAGA+TGG | + | chr2.1:6192670-6192689 | MS.gene45553:CDS | 50.0% | |
| GATACCGCAGCAAGAAGGAT+AGG | + | chr2.1:6193544-6193563 | MS.gene45553:CDS | 50.0% | |
| GGTTGGGCTTCTTATAGCAG+TGG | + | chr2.1:6192771-6192790 | MS.gene45553:CDS | 50.0% | |
| GTCCACTTCATTCTCTGCCA+AGG | - | chr2.1:6192742-6192761 | None:intergenic | 50.0% | |
| TATCAAGAGGCCACAAGGGA+GGG | + | chr2.1:6193701-6193720 | MS.gene45553:CDS | 50.0% | |
| TCCTTAGACCCTACCTCCAT+TGG | + | chr2.1:6193678-6193697 | MS.gene45553:CDS | 50.0% | |
| TGGCCTCTTGATACCAATGG+AGG | - | chr2.1:6193694-6193713 | None:intergenic | 50.0% | |
| ! | ACTTTTGGCCGCAGTCTAGT+GGG | + | chr2.1:6193355-6193374 | MS.gene45553:CDS | 50.0% |
| ! | GTGATGGAGGGAGAATGGAA+GGG | + | chr2.1:6193295-6193314 | MS.gene45553:CDS | 50.0% |
| !! | GATGGTGGGAAGGGGAAAAA+AGG | + | chr2.1:6192712-6192731 | MS.gene45553:CDS | 50.0% |
| !! | GTTCTTCTGGTTTGGGTGGT+GGG | + | chr2.1:6192416-6192435 | MS.gene45553:CDS | 50.0% |
| !! | TAGGGGAACTTGTTGTCAGG+TGG | + | chr2.1:6192993-6193012 | MS.gene45553:CDS | 50.0% |
| !! | TGGTAGGGGAACTTGTTGTC+AGG | + | chr2.1:6192990-6193009 | MS.gene45553:CDS | 50.0% |
| !!! | GGTTTTTGAGTGGTCCCACT+GGG | + | chr2.1:6192437-6192456 | MS.gene45553:CDS | 50.0% |
| !!! | GTTTTTGAGTGGTCCCACTG+GGG | + | chr2.1:6192438-6192457 | MS.gene45553:CDS | 50.0% |
| !!! | TTGGGTGGTGGGTTTTTGAG+TGG | + | chr2.1:6192427-6192446 | MS.gene45553:CDS | 50.0% |
| !!! | TTTTTGAGTGGTCCCACTGG+GGG | + | chr2.1:6192439-6192458 | MS.gene45553:CDS | 50.0% |
| AAGACCCTGCAAAGTCACCG+TGG | + | chr2.1:6193415-6193434 | MS.gene45553:CDS | 55.0% | |
| AAGGTCCAAGATACCCCCAG+TGG | - | chr2.1:6192455-6192474 | None:intergenic | 55.0% | |
| ACCCTTCTTCTGTGACACCC+CGG | - | chr2.1:6192854-6192873 | None:intergenic | 55.0% | |
| AGACCCTGCAAAGTCACCGT+GGG | + | chr2.1:6193416-6193435 | MS.gene45553:CDS | 55.0% | |
| AGCAGGAGGCAGAGTTCAGT+AGG | + | chr2.1:6193646-6193665 | MS.gene45553:CDS | 55.0% | |
| AGGTCCAAGATACCCCCAGT+GGG | - | chr2.1:6192454-6192473 | None:intergenic | 55.0% | |
| CACCGTGGGAGAAAAGAGAG+TGG | + | chr2.1:6193430-6193449 | MS.gene45553:CDS | 55.0% | |
| CACTAGACTGCGGCCAAAAG+TGG | - | chr2.1:6193356-6193375 | None:intergenic | 55.0% | |
| CAGCAAGAAGGATAGGGAGC+TGG | + | chr2.1:6193551-6193570 | MS.gene45553:CDS | 55.0% | |
| GCTCCCTATCCTTCTTGCTG+CGG | - | chr2.1:6193551-6193570 | None:intergenic | 55.0% | |
| GTATCAAGAGGCCACAAGGG+AGG | + | chr2.1:6193700-6193719 | MS.gene45553:CDS | 55.0% | |
| TCTCCCACGGTGACTTTGCA+GGG | - | chr2.1:6193422-6193441 | None:intergenic | 55.0% | |
| TTCTCCCACGGTGACTTTGC+AGG | - | chr2.1:6193423-6193442 | None:intergenic | 55.0% | |
| ! | ATCCGGGGTGTCACAGAAGA+AGG | + | chr2.1:6192849-6192868 | MS.gene45553:CDS | 55.0% |
| ! | CACTTTTGGCCGCAGTCTAG+TGG | + | chr2.1:6193354-6193373 | MS.gene45553:CDS | 55.0% |
| ! | TCCGGGGTGTCACAGAAGAA+GGG | + | chr2.1:6192850-6192869 | MS.gene45553:CDS | 55.0% |
| !! | ACGTGGTGATGGAGGGAGAA+TGG | + | chr2.1:6193290-6193309 | MS.gene45553:CDS | 55.0% |
| !! | GGTGATGGAGGGAGAATGGA+AGG | + | chr2.1:6193294-6193313 | MS.gene45553:CDS | 55.0% |
| !! | GGTTCTTCTGGTTTGGGTGG+TGG | + | chr2.1:6192415-6192434 | MS.gene45553:CDS | 55.0% |
| !! | GTGAGGAGGATGGCCACTTT+TGG | + | chr2.1:6193340-6193359 | MS.gene45553:CDS | 55.0% |
| !!! | GGGTTTTTGAGTGGTCCCAC+TGG | + | chr2.1:6192436-6192455 | MS.gene45553:CDS | 55.0% |
| GCTGAAGCAGAGAGAGCAGG+AGG | + | chr2.1:6193632-6193651 | MS.gene45553:CDS | 60.0% | |
| GGATAGGGAGCTGGAGAAGC+TGG | + | chr2.1:6193560-6193579 | MS.gene45553:CDS | 60.0% | |
| !! | TGGTCCCACTGGGGGTATCT+TGG | + | chr2.1:6192447-6192466 | MS.gene45553:CDS | 60.0% |
| GGAGCTGGAGAAGCTGGCGA+TGG | + | chr2.1:6193566-6193585 | MS.gene45553:CDS | 65.0% | |
| TGTGTCGCCGCAGCAGTGTG+AGG | + | chr2.1:6192918-6192937 | MS.gene45553:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 6192409 | 6193752 | 6192409 | ID=MS.gene45553 |
| chr2.1 | mRNA | 6192409 | 6193752 | 6192409 | ID=MS.gene45553.t1;Parent=MS.gene45553 |
| chr2.1 | exon | 6192409 | 6193752 | 6192409 | ID=MS.gene45553.t1.exon1;Parent=MS.gene45553.t1 |
| chr2.1 | CDS | 6192409 | 6193752 | 6192409 | ID=cds.MS.gene45553.t1;Parent=MS.gene45553.t1 |
| Gene Sequence |
| Protein sequence |
