AlfalfaGEDB | MS.gene57694

M. sativa cultivar XinJiangDaYe / MS.gene57694

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57694.t1	XP_003594968.1	97.9	145	3	0	1	145	1	145	2.10E-75	291.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57694.t1	Q39048	44.4	153	69	6	7	146	1	150	5.5e-29	128.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57694.t1	G7IN16	97.9	145	3	0	1	145	1	145	1.5e-75	291.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene57694	0.805816	1.11E-49	-1.69E-46
MS.gene049396	MS.gene57694	0.842052	3.27E-58	-1.69E-46
MS.gene049907	MS.gene57694	0.800463	1.42E-48	-1.69E-46
MS.gene050375	MS.gene57694	0.81227	4.61E-51	-1.69E-46
MS.gene050499	MS.gene57694	0.835122	2.02E-56	-1.69E-46
MS.gene050502	MS.gene57694	0.834831	2.39E-56	-1.69E-46
MS.gene050520	MS.gene57694	0.826619	2.46E-54	-1.69E-46
MS.gene050593	MS.gene57694	0.802459	5.55E-49	-1.69E-46
MS.gene050662	MS.gene57694	0.813823	2.11E-51	-1.69E-46
MS.gene050686	MS.gene57694	0.80739	5.17E-50	-1.69E-46
MS.gene050772	MS.gene57694	0.805942	1.05E-49	-1.69E-46
MS.gene05105	MS.gene57694	0.816204	6.24E-52	-1.69E-46
MS.gene051255	MS.gene57694	0.826611	2.47E-54	-1.69E-46
MS.gene051387	MS.gene57694	0.815979	7.00E-52	-1.69E-46
MS.gene051509	MS.gene57694	0.832696	8.16E-56	-1.69E-46
MS.gene051941	MS.gene57694	0.829772	4.27E-55	-1.69E-46
MS.gene052346	MS.gene57694	0.837574	4.80E-57	-1.69E-46
MS.gene052999	MS.gene57694	0.866713	2.33E-65	-1.69E-46
MS.gene053169	MS.gene57694	0.837596	4.73E-57	-1.69E-46
MS.gene05335	MS.gene57694	0.803106	4.08E-49	-1.69E-46
MS.gene053449	MS.gene57694	0.823811	1.13E-53	-1.69E-46
MS.gene053505	MS.gene57694	0.843518	1.33E-58	-1.69E-46
MS.gene053574	MS.gene57694	0.811785	5.89E-51	-1.69E-46
MS.gene053758	MS.gene57694	0.815231	1.03E-51	-1.69E-46
MS.gene053813	MS.gene57694	0.875143	3.89E-68	-1.69E-46
MS.gene053912	MS.gene57694	0.827909	1.21E-54	-1.69E-46
MS.gene054486	MS.gene57694	0.844367	7.90E-59	-1.69E-46
MS.gene054556	MS.gene57694	0.802083	6.64E-49	-1.69E-46
MS.gene05492	MS.gene57694	0.802922	4.46E-49	-1.69E-46
MS.gene055722	MS.gene57694	0.806555	7.77E-50	-1.69E-46
MS.gene055741	MS.gene57694	0.821755	3.41E-53	-1.69E-46
MS.gene055744	MS.gene57694	0.820772	5.75E-53	-1.69E-46
MS.gene055909	MS.gene57694	0.862254	5.77E-64	-1.69E-46
MS.gene055911	MS.gene57694	0.867254	1.57E-65	-1.69E-46
MS.gene05601	MS.gene57694	0.839311	1.71E-57	-1.69E-46
MS.gene056166	MS.gene57694	0.839253	1.77E-57	-1.69E-46
MS.gene056422	MS.gene57694	0.846761	1.77E-59	-1.69E-46
MS.gene056511	MS.gene57694	0.834938	2.24E-56	-1.69E-46
MS.gene057019	MS.gene57694	0.834135	3.57E-56	-1.69E-46
MS.gene057076	MS.gene57694	0.81911	1.38E-52	-1.69E-46
MS.gene057086	MS.gene57694	0.802422	5.65E-49	-1.69E-46
MS.gene057734	MS.gene57694	0.806921	6.50E-50	-1.69E-46
MS.gene057749	MS.gene57694	0.821285	4.38E-53	-1.69E-46
MS.gene057823	MS.gene57694	0.82659	2.50E-54	-1.69E-46
MS.gene058120	MS.gene57694	0.805906	1.06E-49	-1.69E-46
MS.gene058459	MS.gene57694	0.807016	6.21E-50	-1.69E-46
MS.gene058700	MS.gene57694	0.806745	7.09E-50	-1.69E-46
MS.gene058995	MS.gene57694	0.838734	2.41E-57	-1.69E-46
MS.gene059129	MS.gene57694	0.827286	1.70E-54	-1.69E-46
MS.gene059367	MS.gene57694	0.809034	2.31E-50	-1.69E-46
MS.gene059368	MS.gene57694	0.83042	2.97E-55	-1.69E-46
MS.gene059455	MS.gene57694	0.817945	2.53E-52	-1.69E-46
MS.gene059674	MS.gene57694	0.854123	1.52E-61	-1.69E-46
MS.gene059680	MS.gene57694	0.838497	2.78E-57	-1.69E-46
MS.gene059711	MS.gene57694	0.859987	2.83E-63	-1.69E-46
MS.gene059807	MS.gene57694	0.815871	7.40E-52	-1.69E-46
MS.gene060372	MS.gene57694	0.846094	2.70E-59	-1.69E-46
MS.gene060401	MS.gene57694	0.860433	2.07E-63	-1.69E-46
MS.gene060482	MS.gene57694	0.806804	6.89E-50	-1.69E-46
MS.gene060835	MS.gene57694	0.808481	3.03E-50	-1.69E-46
MS.gene060837	MS.gene57694	0.847212	1.33E-59	-1.69E-46
MS.gene06097	MS.gene57694	0.808883	2.49E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57694.t1	MTR_2g036780	97.931	145	3	0	1	145	1	145	9.43e-101	296
MS.gene57694.t1	MTR_3g100150	84.828	145	21	1	1	145	1	144	2.81e-85	257
MS.gene57694.t1	MTR_1g035890	70.748	147	40	2	1	145	1	146	2.89e-68	213
MS.gene57694.t1	MTR_8g081480	51.515	132	62	2	16	146	16	146	8.20e-40	131

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57694.t1	AT4G24510	44.444	153	69	6	7	146	1	150	9.21e-37	130
MS.gene57694.t1	AT3G23840	42.177	147	83	2	1	146	1	146	1.63e-33	122
MS.gene57694.t1	AT4G13840	43.796	137	75	2	11	146	13	148	2.36e-32	119

Find 32 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAGGAGGTGCTTCTAAAT+TGG	0.232854	2.3:+51962663	None:intergenic
TCTTCACCTCGTGGTGTTGC+TGG	0.285592	2.3:+51962613	None:intergenic
TCAACAAATCCATGTAGTTT+AGG	0.289089	2.3:+51962575	None:intergenic
TTCGATTCTGAAGCTGTTCA+AGG	0.344410	2.3:-51962520	MS.gene57694:CDS
GATAAACGGTCTACCGGATT+CGG	0.346628	2.3:+51962417	None:intergenic
TTCAGAATCGAAGAAATAAA+TGG	0.359775	2.3:+51962531	None:intergenic
ACACCACGAGGTGAAGAAAA+AGG	0.368645	2.3:-51962607	MS.gene57694:CDS
GATTTGTACTCGCATGTTGC+CGG	0.368832	2.3:-51962454	MS.gene57694:CDS
AACACCGATGTTTCCACTGC+TGG	0.384086	2.3:-51962476	MS.gene57694:CDS
GTTGAAATTCTTGAACTTGA+AGG	0.392731	2.3:+51962643	None:intergenic
GCATCATTGCATTTGATAAA+CGG	0.409324	2.3:+51962403	None:intergenic
GGAGGAGGTGCTTCTAAATT+GGG	0.461109	2.3:+51962664	None:intergenic
TGATGAAGCTTCACTATGTC+CGG	0.472252	2.3:-51962555	MS.gene57694:CDS
GCATTTGATAAACGGTCTAC+CGG	0.498687	2.3:+51962411	None:intergenic
CTTGTTCATGATCATGTGCT+TGG	0.507531	2.3:-51962301	MS.gene57694:CDS
CGAGTTAGAATAGCCGAATC+CGG	0.533458	2.3:-51962430	MS.gene57694:CDS
GTGATAAAACGCTGAGGGAA+TGG	0.545873	2.3:-51962354	MS.gene57694:CDS
TTCGGCTATTCTAACTCGAC+CGG	0.556767	2.3:+51962435	None:intergenic
AATCGAAGAAATAAATGGAC+CGG	0.556767	2.3:+51962536	None:intergenic
ATTCTTGAACTTGAAGGAGG+AGG	0.570601	2.3:+51962649	None:intergenic
GAAATTCTTGAACTTGAAGG+AGG	0.575868	2.3:+51962646	None:intergenic
TGAACACAACCTAAACTACA+TGG	0.576874	2.3:-51962584	MS.gene57694:CDS
CGACTCCGCGATTCGTACAC+CGG	0.579695	2.3:+51962381	None:intergenic
TAAAACGCTGAGGGAATGGT+TGG	0.584184	2.3:-51962350	MS.gene57694:CDS
TTTATCAAATGCAATGATGC+CGG	0.584647	2.3:-51962400	MS.gene57694:CDS
TCGTGGTGTTGCTGGAACAA+CGG	0.592050	2.3:+51962621	None:intergenic
TGCGAGTACAAATCCAGCAG+TGG	0.640512	2.3:+51962463	None:intergenic
AAATCCAGCAGTGGAAACAT+CGG	0.641078	2.3:+51962472	None:intergenic
CGCATTGTGATAAAACGCTG+AGG	0.641466	2.3:-51962360	MS.gene57694:CDS
TGATGCCGGTGTACGAATCG+CGG	0.643478	2.3:-51962386	MS.gene57694:CDS
GCATTGTGATAAAACGCTGA+GGG	0.658570	2.3:-51962359	MS.gene57694:CDS
GTTGTTCCAGCAACACCACG+AGG	0.756952	2.3:-51962619	MS.gene57694:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTGATAAATTGAAATTTT+AGG	-	chr2.3:51962671-51962690	MS.gene57694:CDS	10.0%
!!	AATAATATGATGAAAAAAGT+AGG	+	chr2.3:51962637-51962656	None:intergenic	15.0%
!!	ATTTCAATTTATCAATAACA+CGG	+	chr2.3:51962668-51962687	None:intergenic	15.0%
!!!	TTTTTAAATCGGATATTGTA+AGG	+	chr2.3:51962346-51962365	None:intergenic	20.0%
!	TTCAGAATCGAAGAAATAAA+TGG	+	chr2.3:51962312-51962331	None:intergenic	25.0%
!!!	AAACATCGGTGTTTTTAAAT+CGG	+	chr2.3:51962357-51962376	None:intergenic	25.0%
	AATCGAAGAAATAAATGGAC+CGG	+	chr2.3:51962307-51962326	None:intergenic	30.0%
	GCATCATTGCATTTGATAAA+CGG	+	chr2.3:51962440-51962459	None:intergenic	30.0%
	GTTGAAATTCTTGAACTTGA+AGG	+	chr2.3:51962200-51962219	None:intergenic	30.0%
	TCAACAAATCCATGTAGTTT+AGG	+	chr2.3:51962268-51962287	None:intergenic	30.0%
	TTTATCAAATGCAATGATGC+CGG	-	chr2.3:51962440-51962459	MS.gene57694:CDS	30.0%
	AAAGGAGAAAAAGCAAGATC+AGG	+	chr2.3:51962566-51962585	None:intergenic	35.0%
	GAAATTCTTGAACTTGAAGG+AGG	+	chr2.3:51962197-51962216	None:intergenic	35.0%
	TGAACACAACCTAAACTACA+TGG	-	chr2.3:51962256-51962275	MS.gene57694:intron	35.0%
!!!	TGATCTTGCTTTTTCTCCTT+TGG	-	chr2.3:51962565-51962584	MS.gene57694:CDS	35.0%
!!!	TTCTCCTTTGGTTTTTGTCA+AGG	-	chr2.3:51962577-51962596	MS.gene57694:CDS	35.0%
	AAATCCAGCAGTGGAAACAT+CGG	+	chr2.3:51962371-51962390	None:intergenic	40.0%
	ATTCTTGAACTTGAAGGAGG+AGG	+	chr2.3:51962194-51962213	None:intergenic	40.0%
	CGTACCTTGACAAAAACCAA+AGG	+	chr2.3:51962584-51962603	None:intergenic	40.0%
	CTTGTTCATGATCATGTGCT+TGG	-	chr2.3:51962539-51962558	MS.gene57694:CDS	40.0%
	GCATTGTGATAAAACGCTGA+GGG	-	chr2.3:51962481-51962500	MS.gene57694:CDS	40.0%
	GCATTTGATAAACGGTCTAC+CGG	+	chr2.3:51962432-51962451	None:intergenic	40.0%
	TGATGAAGCTTCACTATGTC+CGG	-	chr2.3:51962285-51962304	MS.gene57694:CDS	40.0%
!!	TTCGATTCTGAAGCTGTTCA+AGG	-	chr2.3:51962320-51962339	MS.gene57694:CDS	40.0%
	ACACCACGAGGTGAAGAAAA+AGG	-	chr2.3:51962233-51962252	MS.gene57694:intron	45.0%
	CGAGTTAGAATAGCCGAATC+CGG	-	chr2.3:51962410-51962429	MS.gene57694:CDS	45.0%
	CGCATTGTGATAAAACGCTG+AGG	-	chr2.3:51962480-51962499	MS.gene57694:CDS	45.0%
	GATAAACGGTCTACCGGATT+CGG	+	chr2.3:51962426-51962445	None:intergenic	45.0%
	GATTTGTACTCGCATGTTGC+CGG	-	chr2.3:51962386-51962405	MS.gene57694:CDS	45.0%
	GTGATAAAACGCTGAGGGAA+TGG	-	chr2.3:51962486-51962505	MS.gene57694:CDS	45.0%
	TAAAACGCTGAGGGAATGGT+TGG	-	chr2.3:51962490-51962509	MS.gene57694:CDS	45.0%
	TTCGGCTATTCTAACTCGAC+CGG	+	chr2.3:51962408-51962427	None:intergenic	45.0%
!	TCACCTTTTTCTTCACCTCG+TGG	+	chr2.3:51962239-51962258	None:intergenic	45.0%
!!	AGGAGGAGGTGCTTCTAAAT+TGG	+	chr2.3:51962180-51962199	None:intergenic	45.0%
!!	GGAGGAGGTGCTTCTAAATT+GGG	+	chr2.3:51962179-51962198	None:intergenic	45.0%
	AACACCGATGTTTCCACTGC+TGG	-	chr2.3:51962364-51962383	MS.gene57694:CDS	50.0%
	TGCGAGTACAAATCCAGCAG+TGG	+	chr2.3:51962380-51962399	None:intergenic	50.0%
!!	TCGTGGTGTTGCTGGAACAA+CGG	+	chr2.3:51962222-51962241	None:intergenic	50.0%
	GTTGTTCCAGCAACACCACG+AGG	-	chr2.3:51962221-51962240	MS.gene57694:intron	55.0%
	TGATGCCGGTGTACGAATCG+CGG	-	chr2.3:51962454-51962473	MS.gene57694:CDS	55.0%
!!	TCTTCACCTCGTGGTGTTGC+TGG	+	chr2.3:51962230-51962249	None:intergenic	55.0%
	CGACTCCGCGATTCGTACAC+CGG	+	chr2.3:51962462-51962481	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	51962164	51962698	51962164	ID=MS.gene57694
chr2.3	mRNA	51962164	51962698	51962164	ID=MS.gene57694.t1;Parent=MS.gene57694
chr2.3	exon	51962264	51962698	51962264	ID=MS.gene57694.t1.exon1;Parent=MS.gene57694.t1
chr2.3	CDS	51962264	51962698	51962264	ID=cds.MS.gene57694.t1;Parent=MS.gene57694.t1
chr2.3	exon	51962164	51962169	51962164	ID=MS.gene57694.t1.exon2;Parent=MS.gene57694.t1
chr2.3	CDS	51962164	51962169	51962164	ID=cds.MS.gene57694.t1;Parent=MS.gene57694.t1

Gene Sequence

>MS.gene57694

ATGGGTTCACTACCCAATTTAGAAGCACCTCCTCCTTCAAGTTCAAGAATTTCAACCGTTGTTCCAGCAACACCACGAGGTGAAGAAAAAGGTGAACACAACCTAAACTACATGGATTTGTTGATGAAGCTTCACTATGTCCGGTCCATTTATTTCTTCGATTCTGAAGCTGTTCAAGGCCTTACAATATCCGATTTAAAAACACCGATGTTTCCACTGCTGGATTTGTACTCGCATGTTGCCGGTCGAGTTAGAATAGCCGAATCCGGTAGACCGTTTATCAAATGCAATGATGCCGGTGTACGAATCGCGGAGTCGCATTGTGATAAAACGCTGAGGGAATGGTTGGATGAAAATGAGTATTCAGTTGATGAACTTGTTCATGATCATGTGCTTGGTCCTGATCTTGCTTTTTCTCCTTTGGTTTTTGTCAAGGTACGTACTAAGATGAATGCACGTCATATCTCATACCTACTTTTTTCATCATATTATTATTGTCAACCGTGTTATTGATAAATTGAAATTTTAGGTTTAG

Protein sequence

>MS.gene57694.t1

MGSLPNLEAPPPSSSRISTVVPATPRGEEKGEHNLNYMDLLMKLHYVRSIYFFDSEAVQGLTISDLKTPMFPLLDLYSHVAGRVRIAESGRPFIKCNDAGVRIAESHCDKTLREWLDENEYSVDELVHDHVLGPDLAFSPLVFVKV