Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61428.t1 | XP_013453058.1 | 92 | 374 | 22 | 4 | 1 | 370 | 1 | 370 | 3.20E-165 | 591.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61428.t1 | Q9LJG8 | 39.8 | 98 | 53 | 2 | 127 | 221 | 84 | 178 | 4.8e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61428.t1 | A0A072UC93 | 92.0 | 374 | 22 | 4 | 1 | 370 | 1 | 370 | 2.3e-165 | 591.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61428.t1 | TF | Trihelix |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene61428 | 0.82347 | 1.36E-53 | -1.69E-46 |
| MS.gene049159 | MS.gene61428 | 0.826514 | 2.60E-54 | -1.69E-46 |
| MS.gene049452 | MS.gene61428 | 0.80424 | 2.38E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene61428 | 0.803163 | 3.97E-49 | -1.69E-46 |
| MS.gene049786 | MS.gene61428 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene61428 | 0.805811 | 1.11E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene61428 | 0.845909 | 3.03E-59 | -1.69E-46 |
| MS.gene050759 | MS.gene61428 | 0.80962 | 1.73E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene61428 | 0.804165 | 2.46E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene61428 | 0.80216 | 6.40E-49 | -1.69E-46 |
| MS.gene050826 | MS.gene61428 | 0.814472 | 1.51E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene61428 | 0.839233 | 1.79E-57 | -1.69E-46 |
| MS.gene050962 | MS.gene61428 | 0.802246 | 6.14E-49 | -1.69E-46 |
| MS.gene051210 | MS.gene61428 | 0.8159 | 7.29E-52 | -1.69E-46 |
| MS.gene051490 | MS.gene61428 | 0.810467 | 1.14E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene61428 | 0.807853 | 4.13E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene61428 | 0.806217 | 9.15E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene61428 | 0.803131 | 4.04E-49 | -1.69E-46 |
| MS.gene052776 | MS.gene61428 | 0.808646 | 2.80E-50 | -1.69E-46 |
| MS.gene052994 | MS.gene61428 | 0.805998 | 1.02E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene61428 | 0.805895 | 1.07E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene61428 | 0.831374 | 1.73E-55 | -1.69E-46 |
| MS.gene054139 | MS.gene61428 | 0.822701 | 2.06E-53 | -1.69E-46 |
| MS.gene054379 | MS.gene61428 | 0.807499 | 4.91E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene61428 | 0.808897 | 2.47E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene61428 | 0.811831 | 5.75E-51 | -1.69E-46 |
| MS.gene055222 | MS.gene61428 | 0.812486 | 4.14E-51 | -1.69E-46 |
| MS.gene055310 | MS.gene61428 | 0.815891 | 7.33E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene61428 | 0.805387 | 1.37E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene61428 | 0.813631 | 2.32E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene61428 | 0.838516 | 2.75E-57 | -1.69E-46 |
| MS.gene055623 | MS.gene61428 | 0.815595 | 8.53E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene61428 | 0.846579 | 1.99E-59 | -1.69E-46 |
| MS.gene055926 | MS.gene61428 | 0.815775 | 7.78E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene61428 | 0.814038 | 1.89E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene61428 | 0.837175 | 6.07E-57 | -1.69E-46 |
| MS.gene056776 | MS.gene61428 | 0.80044 | 1.44E-48 | -1.69E-46 |
| MS.gene056787 | MS.gene61428 | 0.816492 | 5.38E-52 | -1.69E-46 |
| MS.gene056831 | MS.gene61428 | 0.835658 | 1.48E-56 | -1.69E-46 |
| MS.gene057034 | MS.gene61428 | 0.834769 | 2.47E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene61428 | 0.803202 | 3.90E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene61428 | 0.804551 | 2.05E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene61428 | 0.800294 | 1.54E-48 | -1.69E-46 |
| MS.gene058081 | MS.gene61428 | 0.81692 | 4.31E-52 | -1.69E-46 |
| MS.gene058292 | MS.gene61428 | 0.826979 | 2.01E-54 | -1.69E-46 |
| MS.gene058595 | MS.gene61428 | 0.809155 | 2.18E-50 | -1.69E-46 |
| MS.gene059283 | MS.gene61428 | 0.833978 | 3.91E-56 | -1.69E-46 |
| MS.gene059439 | MS.gene61428 | 0.818562 | 1.84E-52 | -1.69E-46 |
| MS.gene059570 | MS.gene61428 | 0.82974 | 4.35E-55 | -1.69E-46 |
| MS.gene059725 | MS.gene61428 | 0.806761 | 7.03E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene61428 | 0.804104 | 2.53E-49 | -1.69E-46 |
| MS.gene060191 | MS.gene61428 | 0.802549 | 5.32E-49 | -1.69E-46 |
| MS.gene060296 | MS.gene61428 | 0.844025 | 9.76E-59 | -1.69E-46 |
| MS.gene06052 | MS.gene61428 | 0.850397 | 1.74E-60 | -1.69E-46 |
| MS.gene060637 | MS.gene61428 | 0.853693 | 2.02E-61 | -1.69E-46 |
| MS.gene060683 | MS.gene61428 | 0.80186 | 7.38E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene61428 | 0.803698 | 3.08E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene61428 | 0.825725 | 4.01E-54 | -1.69E-46 |
| MS.gene060802 | MS.gene61428 | 0.832119 | 1.13E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene61428 | 0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene060850 | MS.gene61428 | 0.801642 | 8.18E-49 | -1.69E-46 |
| MS.gene060851 | MS.gene61428 | 0.80078 | 1.23E-48 | -1.69E-46 |
| MS.gene060870 | MS.gene61428 | 0.816525 | 5.29E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene61428 | 0.832261 | 1.05E-55 | -1.69E-46 |
| MS.gene060998 | MS.gene61428 | 0.81812 | 2.31E-52 | -1.69E-46 |
| MS.gene06121 | MS.gene61428 | 0.813693 | 2.25E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61428.t1 | MTR_6g486270 | 94.385 | 374 | 13 | 4 | 1 | 370 | 1 | 370 | 0.0 | 541 |
| MS.gene61428.t1 | MTR_2g102227 | 51.316 | 228 | 99 | 5 | 126 | 348 | 273 | 493 | 3.64e-57 | 194 |
| MS.gene61428.t1 | MTR_8g022290 | 27.985 | 268 | 145 | 6 | 127 | 347 | 68 | 334 | 2.53e-20 | 91.7 |
| MS.gene61428.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 113 | 216 | 5 | 124 | 9.53e-17 | 80.5 |
| MS.gene61428.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 113 | 216 | 5 | 124 | 1.39e-16 | 80.1 |
| MS.gene61428.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 113 | 216 | 5 | 124 | 1.82e-16 | 79.7 |
| MS.gene61428.t1 | MTR_1g061600 | 38.235 | 102 | 60 | 2 | 125 | 225 | 131 | 230 | 6.45e-16 | 79.3 |
| MS.gene61428.t1 | MTR_2g016140 | 35.106 | 94 | 56 | 2 | 127 | 216 | 23 | 115 | 4.31e-15 | 75.9 |
| MS.gene61428.t1 | MTR_2g016140 | 35.106 | 94 | 56 | 2 | 127 | 216 | 23 | 115 | 7.23e-15 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61428.t1 | AT3G24490 | 44.944 | 356 | 151 | 8 | 1 | 356 | 1 | 311 | 2.04e-82 | 254 |
| MS.gene61428.t1 | AT3G58630 | 27.055 | 292 | 156 | 7 | 109 | 347 | 7 | 294 | 7.34e-27 | 108 |
| MS.gene61428.t1 | AT3G58630 | 27.055 | 292 | 156 | 7 | 109 | 347 | 7 | 294 | 1.10e-26 | 108 |
| MS.gene61428.t1 | AT3G10030 | 35.714 | 154 | 93 | 4 | 68 | 219 | 103 | 252 | 5.74e-19 | 88.6 |
| MS.gene61428.t1 | AT3G10030 | 35.714 | 154 | 93 | 4 | 68 | 219 | 103 | 252 | 9.21e-19 | 88.2 |
| MS.gene61428.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 127 | 345 | 24 | 232 | 4.65e-18 | 83.2 |
| MS.gene61428.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 127 | 345 | 24 | 232 | 7.48e-18 | 82.4 |
| MS.gene61428.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 127 | 345 | 24 | 232 | 8.85e-18 | 82.4 |
| MS.gene61428.t1 | AT3G14180 | 37.405 | 131 | 65 | 4 | 98 | 221 | 58 | 178 | 1.34e-17 | 84.3 |
| MS.gene61428.t1 | AT1G54060 | 28.462 | 260 | 141 | 8 | 127 | 345 | 94 | 349 | 2.58e-16 | 80.1 |
| MS.gene61428.t1 | AT3G11100 | 32.174 | 230 | 129 | 6 | 127 | 345 | 22 | 235 | 5.13e-16 | 77.4 |
| MS.gene61428.t1 | AT3G24860 | 35.870 | 92 | 53 | 1 | 127 | 218 | 65 | 150 | 6.18e-13 | 69.3 |
Find 90 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCGATTCAGAAATT+TGG | 0.112221 | 6.3:-79668498 | MS.gene61428:CDS |
| GTTGCGGAAGAAGCAGATTT+TGG | 0.210644 | 6.3:-79668388 | MS.gene61428:CDS |
| TGGGATTAAGTTCTTGTAAA+TGG | 0.218020 | 6.3:-79668779 | MS.gene61428:CDS |
| GGAGGCTTCGTATAGGATTT+TGG | 0.224205 | 6.3:-79668520 | MS.gene61428:CDS |
| CTTGCTTGTGGTGTTGATTC+GGG | 0.238266 | 6.3:-79668699 | MS.gene61428:CDS |
| GGTGAGAAGTTTCTTCAAAT+TGG | 0.239028 | 6.3:-79668957 | MS.gene61428:CDS |
| CTGATTCGATTCAGAAATTT+GGG | 0.270259 | 6.3:-79668497 | MS.gene61428:CDS |
| CGTTTGTGTTGTTAGAGATA+TGG | 0.280545 | 6.3:-79668980 | MS.gene61428:CDS |
| TCGAGTTCTGTCCATTCCTC+TGG | 0.317779 | 6.3:-79669029 | MS.gene61428:CDS |
| AGAATGGATGAGATGGGTTT+GGG | 0.330760 | 6.3:-79668798 | MS.gene61428:CDS |
| AGACGATGAACCCGAAGAAT+TGG | 0.331680 | 6.3:-79669237 | MS.gene61428:CDS |
| TGATATTGTAAGTTACGAAT+TGG | 0.332259 | 6.3:+79669290 | None:intergenic |
| TCTTGCTTGTGGTGTTGATT+CGG | 0.339235 | 6.3:-79668700 | MS.gene61428:CDS |
| GTTATAATCATCCAATTCTT+CGG | 0.346891 | 6.3:+79669226 | None:intergenic |
| TTATAATCATCCAATTCTTC+GGG | 0.361002 | 6.3:+79669227 | None:intergenic |
| GTGAGAAGTTTCTTCAAATT+GGG | 0.369920 | 6.3:-79668956 | MS.gene61428:CDS |
| TAGAATGGATGAGATGGGTT+TGG | 0.395377 | 6.3:-79668799 | MS.gene61428:CDS |
| AACGTAGATGAAGACGGAAT+TGG | 0.404212 | 6.3:-79669206 | MS.gene61428:CDS |
| GATGAAGACGGAATTGGAAA+TGG | 0.406005 | 6.3:-79669200 | MS.gene61428:CDS |
| GAAGAGTAGAATGGATGAGA+TGG | 0.406263 | 6.3:-79668805 | MS.gene61428:CDS |
| GTTTGTGTTGTTAGAGATAT+GGG | 0.412812 | 6.3:-79668979 | MS.gene61428:CDS |
| ATACAAGGGTTTATTTGAAT+AGG | 0.420616 | 6.3:-79668659 | MS.gene61428:CDS |
| GATATTGTAAGTTACGAATT+GGG | 0.429997 | 6.3:+79669291 | None:intergenic |
| GATTCTAGATATCCATCAAA+AGG | 0.456064 | 6.3:-79669356 | MS.gene61428:CDS |
| AAGAGTAGAATGGATGAGAT+GGG | 0.457358 | 6.3:-79668804 | MS.gene61428:CDS |
| TAAGAAAGAGAAGAGTAGAA+TGG | 0.459638 | 6.3:-79668814 | MS.gene61428:CDS |
| TTATGAATTGTTGCCGCCAT+GGG | 0.464247 | 6.3:-79669090 | MS.gene61428:CDS |
| GGAGTTGGAGAAGGTGAGGT+TGG | 0.470646 | 6.3:-79668430 | MS.gene61428:CDS |
| GGCTGAAATATCGAAAATAC+AGG | 0.472979 | 6.3:-79668355 | MS.gene61428:CDS |
| GGAATTGGAAATGGGTATGT+TGG | 0.473631 | 6.3:-79669191 | MS.gene61428:CDS |
| CGAGTTCTGTCCATTCCTCT+GGG | 0.474330 | 6.3:-79669028 | MS.gene61428:CDS |
| CCTTTCAACTTTCAACTCCT+CGG | 0.476050 | 6.3:+79668884 | None:intergenic |
| TACTAAGAGGCAACAGATGA+TGG | 0.477066 | 6.3:-79668451 | MS.gene61428:CDS |
| ATGAAGACGGAATTGGAAAT+GGG | 0.477798 | 6.3:-79669199 | MS.gene61428:CDS |
| CCGAGGAGTTGAAAGTTGAA+AGG | 0.479871 | 6.3:-79668884 | MS.gene61428:CDS |
| ATTCCTCTGGGTCGGAGGAA+TGG | 0.482329 | 6.3:-79669016 | MS.gene61428:CDS |
| TTCTGTCCATTCCTCTGGGT+CGG | 0.485623 | 6.3:-79669024 | MS.gene61428:CDS |
| GTGTAGGAGTGTATTGGATA+AGG | 0.493185 | 6.3:-79668850 | MS.gene61428:CDS |
| AAGAGGAGGAGGCTTCGTAT+AGG | 0.493270 | 6.3:-79668527 | MS.gene61428:CDS |
| GTGTATTGGATAAGGTGAAG+AGG | 0.499755 | 6.3:-79668842 | MS.gene61428:CDS |
| ATTATGAATTGTTGCCGCCA+TGG | 0.507987 | 6.3:-79669091 | MS.gene61428:CDS |
| GAATGGATTTGATGAGATGA+GGG | 0.516784 | 6.3:-79668634 | MS.gene61428:CDS |
| GAGGCAACAGATGATGGAGT+TGG | 0.516973 | 6.3:-79668445 | MS.gene61428:CDS |
| TGATGTCGATAGGGAAGAGG+AGG | 0.521958 | 6.3:-79668541 | MS.gene61428:CDS |
| TGTCGATAGGGAAGAGGAGG+AGG | 0.528458 | 6.3:-79668538 | MS.gene61428:CDS |
| CTTCAATTCAATGGACGACA+TGG | 0.528859 | 6.3:-79669384 | None:intergenic |
| AGAAGATTGAGAATACTAAG+AGG | 0.534145 | 6.3:-79668464 | MS.gene61428:CDS |
| AGTATGTGTTTATGAATACA+AGG | 0.534616 | 6.3:-79668674 | MS.gene61428:CDS |
| CGAATGGATTTGATGAGATG+AGG | 0.535045 | 6.3:-79668635 | MS.gene61428:CDS |
| TTGCTTGTGGTGTTGATTCG+GGG | 0.537559 | 6.3:-79668698 | MS.gene61428:CDS |
| TTAGGGTTCTTGCGTCGTAA+AGG | 0.541176 | 6.3:+79669329 | None:intergenic |
| TCGAGTGCGAGACAAGAGTA+TGG | 0.550953 | 6.3:-79668723 | MS.gene61428:CDS |
| GTTTATTTGAATAGGTCGAA+TGG | 0.551317 | 6.3:-79668651 | MS.gene61428:CDS |
| ATTCCTCCGACCCAGAGGAA+TGG | 0.555282 | 6.3:+79669018 | None:intergenic |
| TTGTTGCCGCCATGGGGCAG+TGG | 0.558563 | 6.3:-79669083 | MS.gene61428:CDS |
| AGAAGTTTCTTCAAATTGGG+AGG | 0.559816 | 6.3:-79668953 | MS.gene61428:CDS |
| GGCACAGTGTAGGAGTGTAT+TGG | 0.561474 | 6.3:-79668856 | MS.gene61428:CDS |
| CGACGAGGATGATGTCGATA+GGG | 0.561576 | 6.3:-79668550 | MS.gene61428:CDS |
| ACAGATGATGGAGTTGGAGA+AGG | 0.562220 | 6.3:-79668439 | MS.gene61428:CDS |
| ATTCTAGATATCCATCAAAA+GGG | 0.562963 | 6.3:-79669355 | MS.gene61428:CDS |
| GCGACGAGGATGATGTCGAT+AGG | 0.568578 | 6.3:-79668551 | MS.gene61428:CDS |
| GGATGATGTCGATAGGGAAG+AGG | 0.569952 | 6.3:-79668544 | MS.gene61428:CDS |
| AAAGGAATGTGGCACAGTGT+AGG | 0.572005 | 6.3:-79668866 | MS.gene61428:CDS |
| TCGACACAGAACCGCCACTA+GGG | 0.582871 | 6.3:+79669114 | None:intergenic |
| GCAGTAGATGATGATGATGA+TGG | 0.584803 | 6.3:-79668330 | MS.gene61428:CDS |
| TGTCCATTCCTCTGGGTCGG+AGG | 0.586004 | 6.3:-79669021 | MS.gene61428:CDS |
| GAGGAATAGTTTGAGATCAG+AGG | 0.586253 | 6.3:-79668934 | MS.gene61428:CDS |
| GGATGATGATGACGAGGATG+AGG | 0.587237 | 6.3:-79668583 | MS.gene61428:CDS |
| TCAGAACTCTCAGACTCACC+AGG | 0.597701 | 6.3:+79668606 | None:intergenic |
| TGGTGAGTCTGAGAGTTCTG+AGG | 0.600777 | 6.3:-79668604 | MS.gene61428:CDS |
| CAAGAGTATGGTCTTGCTTG+TGG | 0.607980 | 6.3:-79668711 | MS.gene61428:CDS |
| TTCGACACAGAACCGCCACT+AGG | 0.617245 | 6.3:+79669113 | None:intergenic |
| TGATGGAGTTGGAGAAGGTG+AGG | 0.617953 | 6.3:-79668434 | MS.gene61428:CDS |
| ATAGTTTGAGATCAGAGGAA+TGG | 0.618950 | 6.3:-79668929 | MS.gene61428:CDS |
| ATGCGAAGTGGCCCTAGTGG+CGG | 0.619751 | 6.3:-79669125 | MS.gene61428:CDS |
| GAAAATTTACCACTGCCCCA+TGG | 0.622030 | 6.3:+79669074 | None:intergenic |
| GTATGTGTTTATGAATACAA+GGG | 0.625375 | 6.3:-79668673 | MS.gene61428:CDS |
| TTAATAGGGAATTGGAGTTG+CGG | 0.626708 | 6.3:-79668404 | MS.gene61428:CDS |
| TGCTTGTGGTGTTGATTCGG+GGG | 0.626912 | 6.3:-79668697 | MS.gene61428:CDS |
| GATTATAACGTAGATGAAGA+CGG | 0.628195 | 6.3:-79669212 | MS.gene61428:CDS |
| TGATGATGATGGTGATGATG+AGG | 0.650198 | 6.3:-79668319 | MS.gene61428:CDS |
| TATGAATTGTTGCCGCCATG+GGG | 0.651070 | 6.3:-79669089 | MS.gene61428:CDS |
| GATGAGATGAGGGATAGTCC+TGG | 0.652387 | 6.3:-79668624 | MS.gene61428:CDS |
| GTTGAAAGTTGAAAGGAATG+TGG | 0.654797 | 6.3:-79668877 | MS.gene61428:CDS |
| TTTGTGTTGTTAGAGATATG+GGG | 0.660660 | 6.3:-79668978 | MS.gene61428:CDS |
| AAAATGCGAAGTGGCCCTAG+TGG | 0.671315 | 6.3:-79669128 | MS.gene61428:CDS |
| TGTCCATTCCTCCGACCCAG+AGG | 0.680725 | 6.3:+79669013 | None:intergenic |
| AGTTGCTGAGAAAGTTTCCG+AGG | 0.682784 | 6.3:-79668901 | MS.gene61428:CDS |
| TTCTGAGGATGATGATGACG+AGG | 0.701853 | 6.3:-79668589 | MS.gene61428:CDS |
| AATTTACCACTGCCCCATGG+CGG | 0.737952 | 6.3:+79669077 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTTGAAGAAAATGTT+GGG | - | chr6.3:79668489-79668508 | MS.gene61428:CDS | 20.0% |
| ! | AGTATGTGTTTATGAATACA+AGG | - | chr6.3:79668984-79669003 | MS.gene61428:CDS | 25.0% |
| ! | ATACAAGGGTTTATTTGAAT+AGG | - | chr6.3:79668999-79669018 | MS.gene61428:CDS | 25.0% |
| ! | ATATCCATCTTCTTGAAAAA+TGG | + | chr6.3:79668905-79668924 | None:intergenic | 25.0% |
| ! | ATTCTAGATATCCATCAAAA+GGG | - | chr6.3:79668303-79668322 | MS.gene61428:CDS | 25.0% |
| ! | GATATTGTAAGTTACGAATT+GGG | + | chr6.3:79668370-79668389 | None:intergenic | 25.0% |
| ! | GTATGTGTTTATGAATACAA+GGG | - | chr6.3:79668985-79669004 | MS.gene61428:CDS | 25.0% |
| ! | GTTATAATCATCCAATTCTT+CGG | + | chr6.3:79668435-79668454 | None:intergenic | 25.0% |
| ! | TGATATTGTAAGTTACGAAT+TGG | + | chr6.3:79668371-79668390 | None:intergenic | 25.0% |
| ! | TTATAATCATCCAATTCTTC+GGG | + | chr6.3:79668434-79668453 | None:intergenic | 25.0% |
| !! | AAATTTTCGAACTTTAATCG+TGG | - | chr6.3:79668599-79668618 | MS.gene61428:CDS | 25.0% |
| !!! | GGAAATTTTGAAGAAAATGT+TGG | - | chr6.3:79668488-79668507 | MS.gene61428:CDS | 25.0% |
| AAAAAGAGAAAAATGCGAAG+TGG | - | chr6.3:79668521-79668540 | MS.gene61428:CDS | 30.0% | |
| AGAAGATTGAGAATACTAAG+AGG | - | chr6.3:79669194-79669213 | MS.gene61428:CDS | 30.0% | |
| GATTATAACGTAGATGAAGA+CGG | - | chr6.3:79668446-79668465 | MS.gene61428:CDS | 30.0% | |
| GATTCTAGATATCCATCAAA+AGG | - | chr6.3:79668302-79668321 | MS.gene61428:CDS | 30.0% | |
| GTGAGAAGTTTCTTCAAATT+GGG | - | chr6.3:79668702-79668721 | MS.gene61428:CDS | 30.0% | |
| GTTTATTTGAATAGGTCGAA+TGG | - | chr6.3:79669007-79669026 | MS.gene61428:CDS | 30.0% | |
| TAAGAAAGAGAAGAGTAGAA+TGG | - | chr6.3:79668844-79668863 | MS.gene61428:CDS | 30.0% | |
| TGGGATTAAGTTCTTGTAAA+TGG | - | chr6.3:79668879-79668898 | MS.gene61428:CDS | 30.0% | |
| ! | CTGATTCGATTCAGAAATTT+GGG | - | chr6.3:79669161-79669180 | MS.gene61428:CDS | 30.0% |
| ! | GTTTGTGTTGTTAGAGATAT+GGG | - | chr6.3:79668679-79668698 | MS.gene61428:CDS | 30.0% |
| ! | TTTGTGTTGTTAGAGATATG+GGG | - | chr6.3:79668680-79668699 | MS.gene61428:CDS | 30.0% |
| !! | GTTGGATTTTAATAGGGAAT+TGG | - | chr6.3:79669246-79669265 | MS.gene61428:CDS | 30.0% |
| AAGAGTAGAATGGATGAGAT+GGG | - | chr6.3:79668854-79668873 | MS.gene61428:CDS | 35.0% | |
| AGAAGTTTCTTCAAATTGGG+AGG | - | chr6.3:79668705-79668724 | MS.gene61428:CDS | 35.0% | |
| ATGAAGACGGAATTGGAAAT+GGG | - | chr6.3:79668459-79668478 | MS.gene61428:CDS | 35.0% | |
| GGCTGAAATATCGAAAATAC+AGG | - | chr6.3:79669303-79669322 | MS.gene61428:CDS | 35.0% | |
| GGTGAGAAGTTTCTTCAAAT+TGG | - | chr6.3:79668701-79668720 | MS.gene61428:CDS | 35.0% | |
| GTTGAAAGTTGAAAGGAATG+TGG | - | chr6.3:79668781-79668800 | MS.gene61428:CDS | 35.0% | |
| TTAATAGGGAATTGGAGTTG+CGG | - | chr6.3:79669254-79669273 | MS.gene61428:CDS | 35.0% | |
| ! | ATAGTTTGAGATCAGAGGAA+TGG | - | chr6.3:79668729-79668748 | MS.gene61428:CDS | 35.0% |
| ! | CGTTTGTGTTGTTAGAGATA+TGG | - | chr6.3:79668678-79668697 | MS.gene61428:CDS | 35.0% |
| ! | GAAGAAGCAGATTTTGGATA+GGG | - | chr6.3:79669276-79669295 | MS.gene61428:CDS | 35.0% |
| ! | GAATGGATTTGATGAGATGA+GGG | - | chr6.3:79669024-79669043 | MS.gene61428:CDS | 35.0% |
| ! | TTTTCGAACTTTAATCGTGG+TGG | - | chr6.3:79668602-79668621 | MS.gene61428:CDS | 35.0% |
| !! | AGGTGAGGTTGGATTTTAAT+AGG | - | chr6.3:79669239-79669258 | MS.gene61428:CDS | 35.0% |
| !! | GCTGATTCGATTCAGAAATT+TGG | - | chr6.3:79669160-79669179 | MS.gene61428:CDS | 35.0% |
| !! | GGTGAGGTTGGATTTTAATA+GGG | - | chr6.3:79669240-79669259 | MS.gene61428:CDS | 35.0% |
| !! | GGTGTTTTTGACGAAGATTA+GGG | + | chr6.3:79668349-79668368 | None:intergenic | 35.0% |
| !!! | ATGGCCATTTTTCAAGAAGA+TGG | - | chr6.3:79668898-79668917 | MS.gene61428:CDS | 35.0% |
| AACGTAGATGAAGACGGAAT+TGG | - | chr6.3:79668452-79668471 | MS.gene61428:CDS | 40.0% | |
| ATTATGAATTGTTGCCGCCA+TGG | - | chr6.3:79668567-79668586 | MS.gene61428:CDS | 40.0% | |
| CCTTTCAACTTTCAACTCCT+CGG | + | chr6.3:79668777-79668796 | None:intergenic | 40.0% | |
| GAAGAGTAGAATGGATGAGA+TGG | - | chr6.3:79668853-79668872 | MS.gene61428:CDS | 40.0% | |
| GATGAAGACGGAATTGGAAA+TGG | - | chr6.3:79668458-79668477 | MS.gene61428:CDS | 40.0% | |
| GGAATTGGAAATGGGTATGT+TGG | - | chr6.3:79668467-79668486 | MS.gene61428:CDS | 40.0% | |
| GTGTATTGGATAAGGTGAAG+AGG | - | chr6.3:79668816-79668835 | MS.gene61428:CDS | 40.0% | |
| TACTAAGAGGCAACAGATGA+TGG | - | chr6.3:79669207-79669226 | MS.gene61428:CDS | 40.0% | |
| TTATGAATTGTTGCCGCCAT+GGG | - | chr6.3:79668568-79668587 | MS.gene61428:CDS | 40.0% | |
| ! | AGAATGGATGAGATGGGTTT+GGG | - | chr6.3:79668860-79668879 | MS.gene61428:CDS | 40.0% |
| ! | CGAATGGATTTGATGAGATG+AGG | - | chr6.3:79669023-79669042 | MS.gene61428:CDS | 40.0% |
| ! | CGTAAAGGATACCCTTTTGA+TGG | + | chr6.3:79668317-79668336 | None:intergenic | 40.0% |
| ! | GAGGAATAGTTTGAGATCAG+AGG | - | chr6.3:79668724-79668743 | MS.gene61428:CDS | 40.0% |
| ! | GCAGTAGATGATGATGATGA+TGG | - | chr6.3:79669328-79669347 | MS.gene61428:CDS | 40.0% |
| ! | GGAAGAAGCAGATTTTGGAT+AGG | - | chr6.3:79669275-79669294 | MS.gene61428:CDS | 40.0% |
| ! | GTGTAGGAGTGTATTGGATA+AGG | - | chr6.3:79668808-79668827 | MS.gene61428:CDS | 40.0% |
| ! | TAGAATGGATGAGATGGGTT+TGG | - | chr6.3:79668859-79668878 | MS.gene61428:CDS | 40.0% |
| !! | GGGTGTTTTTGACGAAGATT+AGG | + | chr6.3:79668350-79668369 | None:intergenic | 40.0% |
| !! | TCTTGCTTGTGGTGTTGATT+CGG | - | chr6.3:79668958-79668977 | MS.gene61428:CDS | 40.0% |
| !! | TGATGATGATGGTGATGATG+AGG | - | chr6.3:79669339-79669358 | MS.gene61428:CDS | 40.0% |
| AAAGGAATGTGGCACAGTGT+AGG | - | chr6.3:79668792-79668811 | MS.gene61428:CDS | 45.0% | |
| AGACGATGAACCCGAAGAAT+TGG | - | chr6.3:79668421-79668440 | MS.gene61428:CDS | 45.0% | |
| AGTTGCTGAGAAAGTTTCCG+AGG | - | chr6.3:79668757-79668776 | MS.gene61428:CDS | 45.0% | |
| CCGAGGAGTTGAAAGTTGAA+AGG | - | chr6.3:79668774-79668793 | MS.gene61428:CDS | 45.0% | |
| GAAAATTTACCACTGCCCCA+TGG | + | chr6.3:79668587-79668606 | None:intergenic | 45.0% | |
| TATGAATTGTTGCCGCCATG+GGG | - | chr6.3:79668569-79668588 | MS.gene61428:CDS | 45.0% | |
| TTAGGGTTCTTGCGTCGTAA+AGG | + | chr6.3:79668332-79668351 | None:intergenic | 45.0% | |
| ! | CAAGAGTATGGTCTTGCTTG+TGG | - | chr6.3:79668947-79668966 | MS.gene61428:CDS | 45.0% |
| ! | GACGAGGATGAGGATTTTGA+TGG | - | chr6.3:79669085-79669104 | MS.gene61428:CDS | 45.0% |
| ! | GGAGGCTTCGTATAGGATTT+TGG | - | chr6.3:79669138-79669157 | MS.gene61428:CDS | 45.0% |
| ! | GTTGCGGAAGAAGCAGATTT+TGG | - | chr6.3:79669270-79669289 | MS.gene61428:CDS | 45.0% |
| ! | TTCTGAGGATGATGATGACG+AGG | - | chr6.3:79669069-79669088 | MS.gene61428:CDS | 45.0% |
| !! | ACAGATGATGGAGTTGGAGA+AGG | - | chr6.3:79669219-79669238 | MS.gene61428:CDS | 45.0% |
| !! | CTTGCTTGTGGTGTTGATTC+GGG | - | chr6.3:79668959-79668978 | MS.gene61428:CDS | 45.0% |
| !! | TTGCTTGTGGTGTTGATTCG+GGG | - | chr6.3:79668960-79668979 | MS.gene61428:CDS | 45.0% |
| AAAATGCGAAGTGGCCCTAG+TGG | - | chr6.3:79668530-79668549 | MS.gene61428:CDS | 50.0% | |
| AAGAGGAGGAGGCTTCGTAT+AGG | - | chr6.3:79669131-79669150 | MS.gene61428:CDS | 50.0% | |
| AATTTACCACTGCCCCATGG+CGG | + | chr6.3:79668584-79668603 | None:intergenic | 50.0% | |
| CGACGAGGATGATGTCGATA+GGG | - | chr6.3:79669108-79669127 | MS.gene61428:CDS | 50.0% | |
| CGAGTTCTGTCCATTCCTCT+GGG | - | chr6.3:79668630-79668649 | MS.gene61428:CDS | 50.0% | |
| GATGAGATGAGGGATAGTCC+TGG | - | chr6.3:79669034-79669053 | MS.gene61428:CDS | 50.0% | |
| GGATGATGTCGATAGGGAAG+AGG | - | chr6.3:79669114-79669133 | MS.gene61428:CDS | 50.0% | |
| TCAGAACTCTCAGACTCACC+AGG | + | chr6.3:79669055-79669074 | None:intergenic | 50.0% | |
| TCGAGTGCGAGACAAGAGTA+TGG | - | chr6.3:79668935-79668954 | MS.gene61428:CDS | 50.0% | |
| TCGAGTTCTGTCCATTCCTC+TGG | - | chr6.3:79668629-79668648 | MS.gene61428:CDS | 50.0% | |
| TGATGGAGTTGGAGAAGGTG+AGG | - | chr6.3:79669224-79669243 | MS.gene61428:CDS | 50.0% | |
| TGATGTCGATAGGGAAGAGG+AGG | - | chr6.3:79669117-79669136 | MS.gene61428:CDS | 50.0% | |
| TGGTGAGTCTGAGAGTTCTG+AGG | - | chr6.3:79669054-79669073 | MS.gene61428:CDS | 50.0% | |
| TTCTGTCCATTCCTCTGGGT+CGG | - | chr6.3:79668634-79668653 | MS.gene61428:CDS | 50.0% | |
| ! | GCAGATTTTGGATAGGGCTC+AGG | - | chr6.3:79669282-79669301 | MS.gene61428:CDS | 50.0% |
| ! | GGATGATGATGACGAGGATG+AGG | - | chr6.3:79669075-79669094 | MS.gene61428:CDS | 50.0% |
| ! | GGCACAGTGTAGGAGTGTAT+TGG | - | chr6.3:79668802-79668821 | MS.gene61428:CDS | 50.0% |
| !! | GAGGCAACAGATGATGGAGT+TGG | - | chr6.3:79669213-79669232 | MS.gene61428:CDS | 50.0% |
| !! | TGCTTGTGGTGTTGATTCGG+GGG | - | chr6.3:79668961-79668980 | MS.gene61428:CDS | 50.0% |
| !!! | TGAGGATTTTGATGGCGACG+AGG | - | chr6.3:79669093-79669112 | MS.gene61428:CDS | 50.0% |
| ATTCCTCCGACCCAGAGGAA+TGG | + | chr6.3:79668643-79668662 | None:intergenic | 55.0% | |
| ATTCCTCTGGGTCGGAGGAA+TGG | - | chr6.3:79668642-79668661 | MS.gene61428:CDS | 55.0% | |
| GCGACGAGGATGATGTCGAT+AGG | - | chr6.3:79669107-79669126 | MS.gene61428:CDS | 55.0% | |
| GGAGTTGGAGAAGGTGAGGT+TGG | - | chr6.3:79669228-79669247 | MS.gene61428:CDS | 55.0% | |
| TCGACACAGAACCGCCACTA+GGG | + | chr6.3:79668547-79668566 | None:intergenic | 55.0% | |
| TGTCGATAGGGAAGAGGAGG+AGG | - | chr6.3:79669120-79669139 | MS.gene61428:CDS | 55.0% | |
| TTCGACACAGAACCGCCACT+AGG | + | chr6.3:79668548-79668567 | None:intergenic | 55.0% | |
| ATGCGAAGTGGCCCTAGTGG+CGG | - | chr6.3:79668533-79668552 | MS.gene61428:CDS | 60.0% | |
| TGTCCATTCCTCCGACCCAG+AGG | + | chr6.3:79668648-79668667 | None:intergenic | 60.0% | |
| TGTCCATTCCTCTGGGTCGG+AGG | - | chr6.3:79668637-79668656 | MS.gene61428:CDS | 60.0% | |
| TTGTTGCCGCCATGGGGCAG+TGG | - | chr6.3:79668575-79668594 | MS.gene61428:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 79668284 | 79669396 | 79668284 | ID=MS.gene61428 |
| chr6.3 | mRNA | 79668284 | 79669396 | 79668284 | ID=MS.gene61428.t1;Parent=MS.gene61428 |
| chr6.3 | exon | 79668284 | 79669396 | 79668284 | ID=MS.gene61428.t1.exon1;Parent=MS.gene61428.t1 |
| chr6.3 | CDS | 79668284 | 79669396 | 79668284 | ID=cds.MS.gene61428.t1;Parent=MS.gene61428.t1 |
| Gene Sequence |
| Protein sequence |
