AlfalfaGEDB | MS.gene65986

M. sativa cultivar XinJiangDaYe / MS.gene65986

KEGG: K12493
GO: GO:0003674 GO:0005096 GO:0008047 GO:0030234 GO:0030695 GO:0060589 GO:0098772
PFAM: PF01412.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65986.t1	XP_003628813.1	89.2	388	41	1	9	396	23	409	1.50E-157	565.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65986.t1	Q8H100	63.7	394	127	6	11	396	28	413	4.7e-107	389.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65986.t1	G7LHQ5	89.2	388	41	1	9	396	23	409	1.1e-157	565.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene65986	-0.808041	3.76E-50	-1.69E-46
MS.gene050170	MS.gene65986	-0.81072	1.00E-50	-1.69E-46
MS.gene050225	MS.gene65986	0.801453	8.94E-49	-1.69E-46
MS.gene050577	MS.gene65986	-0.84706	1.47E-59	-1.69E-46
MS.gene050904	MS.gene65986	-0.846471	2.13E-59	-1.69E-46
MS.gene051317	MS.gene65986	-0.801715	7.90E-49	-1.69E-46
MS.gene052015	MS.gene65986	0.816342	5.81E-52	-1.69E-46
MS.gene052164	MS.gene65986	-0.801068	1.07E-48	-1.69E-46
MS.gene052482	MS.gene65986	-0.815391	9.47E-52	-1.69E-46
MS.gene052486	MS.gene65986	-0.822915	1.83E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene030337	MS.gene65986	PPI
MS.gene038430	MS.gene65986	PPI
MS.gene048438	MS.gene65986	PPI
MS.gene049051	MS.gene65986	PPI
MS.gene049052	MS.gene65986	PPI
MS.gene20137	MS.gene65986	PPI
MS.gene65986	MS.gene05967	PPI
MS.gene65986	MS.gene65987	PPI
MS.gene65986	MS.gene008994	PPI
MS.gene65986	MS.gene015841	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65986.t1	MTR_8g067080	97.686	389	6	3	9	396	23	409	0.0	762
MS.gene65986.t1	MTR_8g471110	97.686	389	6	3	9	396	23	409	0.0	762
MS.gene65986.t1	MTR_8g067080	97.172	389	6	4	9	396	23	407	0.0	753
MS.gene65986.t1	MTR_8g067090	76.227	387	81	5	11	396	25	401	0.0	541
MS.gene65986.t1	MTR_8g471120	76.227	387	81	5	11	396	25	401	0.0	541
MS.gene65986.t1	MTR_7g099830	42.742	124	62	2	11	130	19	137	1.08e-28	116
MS.gene65986.t1	MTR_1g069000	43.243	111	58	1	11	121	19	124	2.88e-26	110
MS.gene65986.t1	MTR_8g464390	55.882	68	30	0	9	76	29	96	4.43e-21	95.1
MS.gene65986.t1	MTR_6g080710	40.351	114	60	1	9	114	29	142	5.44e-21	92.0
MS.gene65986.t1	MTR_2g080950	54.412	68	31	0	9	76	29	96	1.50e-20	93.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65986.t1	AT4G17890	63.452	394	128	7	11	396	28	413	4.69e-167	474
MS.gene65986.t1	AT5G46750	61.637	391	132	10	11	396	25	402	2.18e-155	444
MS.gene65986.t1	AT4G17890	62.604	361	119	7	11	363	28	380	2.21e-148	426
MS.gene65986.t1	AT2G35210	62.141	383	125	11	11	387	25	393	1.85e-144	416
MS.gene65986.t1	AT2G35210	59.451	328	114	10	11	333	25	338	7.89e-116	342
MS.gene65986.t1	AT3G53710	47.748	111	53	1	11	121	19	124	1.50e-29	119
MS.gene65986.t1	AT3G53710	47.748	111	53	1	11	121	19	124	1.50e-29	119
MS.gene65986.t1	AT3G53710	47.748	111	53	1	11	121	19	124	1.50e-29	119
MS.gene65986.t1	AT2G37550	45.946	111	55	1	11	121	19	124	6.70e-29	117
MS.gene65986.t1	AT2G37550	45.946	111	55	1	11	121	19	124	6.70e-29	117

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Find 78 sgRNAs with CRISPR-Local

Find 389 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAACATTGCTGGAGAGAC+TGG	0.349107	8.1:+37433789	MS.gene65986:CDS
AAGAAGCTTGATGATGACTT+TGG	0.488401	8.1:-37430841	None:intergenic
AATAATTTGAAGAAGCCTAT+TGG	0.314657	8.1:+37430400	MS.gene65986:CDS
ACATGGGCGTCTGTAACGTA+TGG	0.500777	8.1:+37428097	MS.gene65986:CDS
ACTGCAGTATATGAAGCTCT+AGG	0.588166	8.1:-37430373	None:intergenic
ACTGGAGCAGGTAGTTCTTC+AGG	0.365053	8.1:-37430685	None:intergenic
ACTTGCACTTTGGAGGAGCT+TGG	0.450343	8.1:-37430907	None:intergenic
AGAACATTGCTGGAGAGACT+GGG	0.589784	8.1:+37433790	MS.gene65986:CDS
AGACCAGAACAAGGCCAATG+CGG	0.704868	8.1:+37432292	MS.gene65986:CDS
AGCAGCAGGTGGTGCAGACA+AGG	0.494559	8.1:-37430233	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACAGTGTTCGCAATTT+CGG	+	chr8.1:37431070-37431089	MS.gene65986:intron	30.0%
AAAACATGCAATTCTACGTA+TGG	-	chr8.1:37431145-37431164	None:intergenic	30.0%
AAAGCATGTTCAAATGCATT+GGG	-	chr8.1:37433159-37433178	None:intergenic	30.0%
AACTCAGTAAAGAATCTCAA+GGG	-	chr8.1:37429469-37429488	None:intergenic	30.0%
AATCACTCTGTTAAGAAACA+TGG	-	chr8.1:37429168-37429187	None:intergenic	30.0%
ACGGTAAGTTACTAAAAGAA+AGG	-	chr8.1:37432355-37432374	None:intergenic	30.0%
ACGTCTATGAAATCATATTG+GGG	+	chr8.1:37429223-37429242	MS.gene65986:intron	30.0%
AGATAAACTTTATAGGTCCA+TGG	+	chr8.1:37431914-37431933	MS.gene65986:intron	30.0%
AGATTCTTTACTGAGTTAAG+AGG	+	chr8.1:37429472-37429491	MS.gene65986:intron	30.0%
AGCAGTTAGTTGTAATGTAA+CGG	-	chr8.1:37431842-37431861	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	37428040	37433872	37428040	ID=MS.gene65986
chr8.1	mRNA	37428040	37433872	37428040	ID=MS.gene65986.t1;Parent=MS.gene65986
chr8.1	exon	37428040	37428185	37428040	ID=MS.gene65986.t1.exon1;Parent=MS.gene65986.t1
chr8.1	CDS	37428040	37428185	37428040	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1
chr8.1	exon	37430033	37430477	37430033	ID=MS.gene65986.t1.exon2;Parent=MS.gene65986.t1
chr8.1	CDS	37430033	37430477	37430033	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1
chr8.1	exon	37430658	37430927	37430658	ID=MS.gene65986.t1.exon3;Parent=MS.gene65986.t1
chr8.1	CDS	37430658	37430927	37430658	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1
chr8.1	exon	37432221	37432343	37432221	ID=MS.gene65986.t1.exon4;Parent=MS.gene65986.t1
chr8.1	CDS	37432221	37432343	37432221	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1
chr8.1	exon	37433542	37433640	37433542	ID=MS.gene65986.t1.exon5;Parent=MS.gene65986.t1
chr8.1	CDS	37433542	37433640	37433542	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1
chr8.1	exon	37433765	37433872	37433765	ID=MS.gene65986.t1.exon6;Parent=MS.gene65986.t1
chr8.1	CDS	37433765	37433872	37433765	ID=cds.MS.gene65986.t1;Parent=MS.gene65986.t1

Gene Sequence

>MS.gene65986

ATGAAGTTGTGGTGGTTTTTGTTTCAGAGTTGTTTCGATTGCAATGCGAAGAATCCAACATGGGCGTCTGTAACGTATGGGATCTTCCTCTGCATTGATTGCTCTGCTGTTCATCGAAGTCTCGGTGTTCACATCAGTTTCGTGAGGTAATTTGTTGATTGATCTTGTGAAATGATGAATTTGTTTACTCATTTTTGAAATTTGATTTTGTATGTTGATGAGGTTTGAATTTGTTTGTTGATTAATTTCTTGAGTAATGTGTTGTTTTGTGGTTATGTGTGTGTGCTGAATTTGTAATTAGATAAGAAGTAAGTAGTTAGTGGGGGTCAGGTAGCTCAACTGGTTTGAGCCAAGTGTTGAAGAAGTTTGATGATCCGAGTCGGTTAAGTGTTTTTGATGATCCTGGTCAAACTCTTGTGAAAAGAAAAATACTATGAGCATGTTTGGATTGACTTATTTGGGCTGATCTACTGACATAAGTTTTGTGAGATTGTTTAGGAGAATTTATGAAAATAGCCTATGATAATTTTCATAAGCTTTTTTTTTGCCTACTTATTTTCATAAGTTCTCCAATGCAGCTCACAAAAATAACTTATAGCATGTACGAAAACAATTTAATGTTATGTTATCTTTTACTATAAAAATAGCTTATGCAAGCTTATATAATAAGCATTTGTGCTATAATATGCAAATAAGTTGTTTATCCAAACATGCCCTTTGTCTATTTGGGAGGTTTTTTTTTGGAGGGTTTTGGAGGGGAGGGCTAAGTTTATATTCTGATATATATTTGATATTTTAAAAAAAATTTAAAGGAACATCATGATTTATGATCGTATAATAGTTCAATCACACTTGGTTGATTTAAAAAATACTTTGTAGTGTCCGTAATTTAAAAAAGAAAAGTAAGCCTTCCTCTCCTAATCCTAAACCCTCCATCCAAACATACCCTAACATAACAATTAACAATTAAAAAAAGTTAGTAATTAGTTGGTGATTTACTAGTAGTTAGTTACACACTTACTTGCTATGTAAGTGATGTAACACCTTTAATGCTACACACTTTCTCATTTTTACTATAGAGTTTCATTGAAGGTTTGTATTATTTTCTCACTTCTATGGGTTCTACCATGTTTCTTAACAGAGTGATTAGTAATTTTCCCAAATCAAATGGTTAAAACTTGAAACGTCTATGAAATCATATTGGGGGTTTTGCCTCTCAGTATTTGTGTAATTGAAATCATTCATTCTACTTTGGAAAGAGTAGAATGGTATTATGCTTGAGAAGCTGTGTAGAATGAATAAATAAATGCTGGCGAATATTTATACCCTAAGTTAGTTTTTGGAGCACAAGGTTAGACATGATTTACTTTTTGCAGCAGGTTGGAATGCAGATATCGTTGTTGATTCTGGTAGCCTTATATTATTTCCCTTGAGATTCTTTACTGAGTTAAGAGGAGTGTTTAGATGGGAGTTTCTTTATTGAGTTAAGAAGAGTGTTTAGATTAGAAAATGATTGATTATGTAATGTAATTTTCTGGGTGCTTTTTGAGTTGCAATGTGTGATAAGTATTTGGAATTTAGAAACTGATTAACTGGTTTAAAATAGATGAAGCAAACTTGGAACCAGCAAAAATTAAGTTTGATTCTGTAAGAGAAAACTTCACTTATTGCCACTGCAAACCAACATTTTAGTTTGGTTTTTCAGAGTATATCATAGGAAAAAGTTGAGCAGGAGCTATATGCCATTATGCCGAGTAAAATTTGTTAGGGTTGATACGCTTAATTTATGAATCAAAAGTAAAAACTATTTCATCAAGCTTCTGTTTTCTCTAACTAAAAATTGAATCTCTCACCCTGTTTTTGTGTCTTTGTTTTATATTTAGTTATTCTATGAATTACATTACAATCAACAGGGATGAATTAGTCCTGTACTGTAGTAGAATACAAGTGAGAAGGCATAGAAGATTTATACTTATATATTTTTTATATGTAGATCTACGAACTTAGACTCGTGGAGTCCTGAGCAACTTAAAATGATGAGCTTTGGAGGAAACAGTAGGGCACAAGTTTTCTTTAGGCAGCATGGATGGAACGGAGATGGTAAAGTAGAGGCAAAGTATACATCCAGAGCTGCAGAGTTGTATAAGCAACTGCTTTCAAAGGAAGTTGCTAAAAGTATGTCTGAGGAGGCTGCCTTGTCTGCACCACCTGCTGCTTCTTCTCAGTCTGCCCAAGGGGCTAATGGACTTCCTGATGTTAAGACAAATGAAGCCCCAATTGAAACTGTGGAAAAAACCGTGGAGAAGCCAGAAAAGACCGAGAGCACTTCTTCACCTAGAGCTTCATATACTGCAGTTTCGAATAATTTGAAGAAGCCTATTGGAGCCAAAAAAACTGGTAAGAGCGGGGGGCTTGGAGCCCGTAAGCTCACTAGAAAGGTATGCAAATAGTTATCTTTGATTAAAGATTGTTTACAGAAAGTTCCTTTTGGTTCATCAGTATCTTGTTCTCTATTTTAATTTGGTGATATAAAATTTTAACTGGTTATTTTTTGTATGTTCTCTATTTTAATTTGGTGATATAAAATTTTAACCAGTTATTATCATAATGCAATGCAGCCAAGTGAGTCCCTCTATGAGCAGAAGCCTGAAGAACTACCTGCTCCAGTTTCATCATCGACAATTACAAAAAACAACTTGCCTTCTGGGCCACCTCTAACATCTCGGTTTGAGTATACAGAAGATGTCCAATCATCTGAGTTGAATCCTGGAGGTTCTAATGTAACCGGTCACGTGTCTGTACCAAAGTCATCATCAAGCTTCTTTTCGGACTTCGGAATGGATAGTGGCTTTCAAAAGAAATCTGGGCCAAGCTCCTCCAAAGTGCAAGTAAGCCTATGCCTGCATCTCTTACCTCTGCATAAATTAACTTCTGCACGTGGTGAGCTTACAGAGTACTTTATACACGATTTAGTTCAAATTTGATATTTTCGCCCAGTTTAATGTTTTTAACAAAATACAATCCTTTGAGAAAAACAGTGTTCGCAATTTCGGGATAAATTACCAGCATTGCAAGTATTTTGCAAAAAAGATGAAATTGCATCCATACGTAGAATTGCATGTTTTCACACCTTGTGCACTTCCAAATTTTTAATGGAACATTATATCCCTTGATTTATTTTACTAGGGATTTCCGTGTAATTTTGAAAACAGAGATTTGTGGCTGAAAACTATGTGTTTGCAAGTGCAGTGATTGTGTACTACTATTATAGGCTTAAATATGCAAATGGTCCCTGTGAATATAAGCAATTTGATTTTAGTCCATGCATTTTTTGTTTTTGTTTTGTTTGTCTTGCAAATATGAAATATTTTGTTTTGGTCCTTGGTGTTTTGTTTTAGTCCCTGTAAAATCTATTCATATTAAAATTGGTCCCTATAATATTTATTTTAGTTCTTATTTTGAGGGACTAAAATCAAATATTCTACATATTACCAAATCCAATATGAATACATTTTGCAAGGACTAAAAGACTAAAAGCAAAAAATTATATATTTGCACGAACAAAAACCAAAAAATATTTTTACAGGGACTAAAACCAAAACGCTTGATATTTCCATTGTTTATTTAAGCTTACTATGATATCATCATTGTGGTCTTGGATGGTTTTGCAGTAGGACCTTCAGTATTATTCAAAACTACAATAGTATTACCATGAGAATGAGATTGCTGAGAGAAGTGCCAGTAATAGAAACAAACTTAAACAAGACATAGATAGAAAACTCTAGAGAAAAAATAACCACCGTTACATTACAACTAACTGCTATAGTATCCCAAAGAAAAGATATGGAATAAAGTAACACATGTATCTGAACAAAGATAAACTTTATAGGTCCATGGGTGTACCTTTTTTAAATTAATTATTAGTAGATGGGCATTATTGCAATCATAGGCTGAATGATATTGATTATCTTTGGACAAATTAGTATTTTTGTTCCGAATCTTAGTGTTATTTGCCTATATGTTATGCCCATGTAATCTTTGAACATTCTTTTATATCTCACCTAGATGTTGCCATTCATCTGAGTGTTTTTCTCAATAAATTCATTCAACCTGCACAATCATATTTTTTATTTTTTACCACAGTTATGCATCTCATTTTGCTTTGTCTGTGTCTGTATAAGATTCAGGAATCTGATGAAGCAAGAAAGAAGTTCTCAAATGCAAAGTCTATTTCTTCATCCCAATTTTTTGGAGACCAGAACAAGGCCAATGCGGATGCTCAAGCAACTTTATCAAAGTTTTCAGTATGTGTCCTTTCTTTTAGTAACTTACCGTAACAAAGTGTTAGTATGTATGCACATGTAGATCATAGATGTTCAATGCCCTGTAGTGGCACTGTATTGGATGACTTCTTTCATGTCACATACTCTTTTGATTGGCAGAATTTATATTACCTACCAAATTTCCAAAATCAACCCTGATAAATTTGATGTCAAACACTCTAATCCTGAATACAGAAGTCCCGCCTTATTAGTCTCTAAGGAACTAATATTAGTCAAATTAGTCTTCAGGCACCAGACCTTACAAGGACTAATATTATTTACATTTGGGATTCATGCATATTAGTATTTAAATTAGCATTTTTGCATTATCAAGGATCACTATTTACGCCATCAAAATTTTCTGGGCAAATTAGGCAAAATAATCTATACTTACACGGAAATTTAAGGGCTAGTGATCTATGTTGTTTTGAAGTGAATATTTCATAATGAAGATAAAATATGAATGATATTTTACTCACAGGTGTTTTTGTAAAGATAAGTTATTCCAGACTTGTTTTATAGGTCACAGTTTTTTTTTCTTCTGTCCGACTGTACTCTGTAGGGATTACAGTTTTACTAGTAAACACCATTGTGGCAAACCATTTATATCATACATAATTGAAGGCCTGAAATTATTTCTGAACACAATGTCTGCGAATTTTTTTGCCTTAATATTAATTAATTGTATAATCTTGTAACCCAATGTTTACGAGTGGAAACTCATTTTTTGGTGCTTTAGTATGGTTTTCTCTTCTGTCAAAATGGGTTGTAGTGCATCAATTTACAGAATTTAACCCAATGCATTTGAACATGCTTTTCCTTCTTGAAAAATATGTCTCTTCTATTCGTTATAATTTATCGGCTAGGGTTGGTTGAGTGGGATGAGTGCGAGAAAGGACAAGTTATGTTCCAAATTTAATCAAGTAATTTTTGGCATCTATTTGGTGTAAAGCAAATTAAGCCATGGAATTTCAGATATTATAGATATAGATATGTTGAGGGATTGGTAAGCTATGTTCCGGTAATCTTCTCTTTTCTTTCTTGGGTATTTTTGTCTTTGTTTTGAAGAAGTTCTTATTCTCCCATGTCTTTGCAACTAGGAATGGAAAAACGAAGAGGGCCCGGGAGAGAGGGAGTTAAATATTGATACTTAACTTGGTCTATCCTTTTATATTTCCAGGGTTCATCTGCCATCTCCAGTGCTGATCTTTTTGGTGACTCGAGTGACAACTCCGTTGATCTTGCTGCTAGTGACCTTATCAATAGAATATCTTTCCAGGTAATCCTTATATTATACTGGACACAAACTTAATGAGCAACGAAGGCATTATTAGATTCTTTGTATTTGTTCTGTGTGGATGTTTTGTGGTATGCTGATACCCTACACCCCTCCCTTATTACAGGCACAACAAGATATCTCTTCCCTTAAGAACATTGCTGGAGAGACTGGGAAAAAACTCACCTCCTTGGCATCCTCCTTGATGACAGATCTTCAGGACCGAATCCTCTAA

Protein sequence

>MS.gene65986.t1

MKLWWFLFQSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVRSTNLDSWSPEQLKMMSFGGNSRAQVFFRQHGWNGDGKVEAKYTSRAAELYKQLLSKEVAKSMSEEAALSAPPAASSQSAQGANGLPDVKTNEAPIETVEKTVEKPEKTESTSSPRASYTAVSNNLKKPIGAKKTGKSGGLGARKLTRKPSESLYEQKPEELPAPVSSSTITKNNLPSGPPLTSRFEYTEDVQSSELNPGGSNVTGHVSVPKSSSSFFSDFGMDSGFQKKSGPSSSKVQIQESDEARKKFSNAKSISSSQFFGDQNKANADAQATLSKFSGSSAISSADLFGDSSDNSVDLAASDLINRISFQAQQDISSLKNIAGETGKKLTSLASSLMTDLQDRIL