AlfalfaGEDB | MS.gene75923

M. sativa cultivar XinJiangDaYe / MS.gene75923

KEGG: K12859
GO: GO:0000375 GO:0000377 GO:0000398 GO:0005575 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0016071 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0046540 GO:0071704 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1990904
PFAM: PF02966.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75923.t1	GAU28190.1	69	142	16	2	1	114	1	142	5.90E-46	193.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75923.t1	Q9FE62	66.9	142	19	2	1	114	1	142	1.6e-47	189.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75923.t1	A0A2Z6MWW0	69.0	142	16	2	1	114	1	142	4.3e-46	193.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049424	MS.gene75923	0.821043	4.98E-53	-1.69E-46
MS.gene049653	MS.gene75923	0.8268	2.22E-54	-1.69E-46
MS.gene050277	MS.gene75923	0.858068	1.06E-62	-1.69E-46
MS.gene050288	MS.gene75923	0.809204	2.12E-50	-1.69E-46
MS.gene050330	MS.gene75923	-0.80444	2.16E-49	-1.69E-46
MS.gene050719	MS.gene75923	0.821476	3.96E-53	-1.69E-46
MS.gene050721	MS.gene75923	0.826748	2.29E-54	-1.69E-46
MS.gene050724	MS.gene75923	0.812816	3.51E-51	-1.69E-46
MS.gene050815	MS.gene75923	0.814018	1.91E-51	-1.69E-46
MS.gene050987	MS.gene75923	0.812337	4.46E-51	-1.69E-46
MS.gene051063	MS.gene75923	0.813281	2.77E-51	-1.69E-46
MS.gene051241	MS.gene75923	0.803097	4.10E-49	-1.69E-46
MS.gene051243	MS.gene75923	0.829228	5.79E-55	-1.69E-46
MS.gene051245	MS.gene75923	0.860764	1.65E-63	-1.69E-46
MS.gene051349	MS.gene75923	0.839055	1.99E-57	-1.69E-46
MS.gene051452	MS.gene75923	0.816389	5.67E-52	-1.69E-46
MS.gene051577	MS.gene75923	0.822312	2.53E-53	-1.69E-46
MS.gene051633	MS.gene75923	0.823009	1.74E-53	-1.69E-46
MS.gene051756	MS.gene75923	0.819574	1.08E-52	-1.69E-46
MS.gene051757	MS.gene75923	0.851849	6.79E-61	-1.69E-46
MS.gene051903	MS.gene75923	0.827795	1.28E-54	-1.69E-46
MS.gene052176	MS.gene75923	0.853739	1.96E-61	-1.69E-46
MS.gene052286	MS.gene75923	0.816942	4.26E-52	-1.69E-46
MS.gene052302	MS.gene75923	0.863896	1.79E-64	-1.69E-46
MS.gene052376	MS.gene75923	0.854421	1.24E-61	-1.69E-46
MS.gene052856	MS.gene75923	0.837167	6.10E-57	-1.69E-46
MS.gene053216	MS.gene75923	0.824299	8.70E-54	-1.69E-46
MS.gene054930	MS.gene75923	0.813435	2.56E-51	-1.69E-46
MS.gene055441	MS.gene75923	0.80423	2.39E-49	-1.69E-46
MS.gene056601	MS.gene75923	0.805943	1.05E-49	-1.69E-46
MS.gene056631	MS.gene75923	0.836619	8.42E-57	-1.69E-46
MS.gene056760	MS.gene75923	0.842061	3.25E-58	-1.69E-46
MS.gene057057	MS.gene75923	0.825406	4.77E-54	-1.69E-46
MS.gene057079	MS.gene75923	0.831839	1.33E-55	-1.69E-46
MS.gene057140	MS.gene75923	0.853348	2.54E-61	-1.69E-46
MS.gene057234	MS.gene75923	0.850622	1.51E-60	-1.69E-46
MS.gene057754	MS.gene75923	0.812406	4.31E-51	-1.69E-46
MS.gene05795	MS.gene75923	0.863219	2.91E-64	-1.69E-46
MS.gene058055	MS.gene75923	0.836701	8.02E-57	-1.69E-46
MS.gene058182	MS.gene75923	0.845758	3.33E-59	-1.69E-46
MS.gene058238	MS.gene75923	0.869841	2.29E-66	-1.69E-46
MS.gene058399	MS.gene75923	0.803889	2.81E-49	-1.69E-46
MS.gene058906	MS.gene75923	0.8809	3.75E-70	-1.69E-46
MS.gene059830	MS.gene75923	0.808947	2.41E-50	-1.69E-46
MS.gene059898	MS.gene75923	0.84033	9.28E-58	-1.69E-46
MS.gene06001	MS.gene75923	0.816432	5.55E-52	-1.69E-46
MS.gene060084	MS.gene75923	0.805326	1.41E-49	-1.69E-46
MS.gene060165	MS.gene75923	0.810118	1.35E-50	-1.69E-46
MS.gene060297	MS.gene75923	0.845046	5.18E-59	-1.69E-46
MS.gene060619	MS.gene75923	0.813962	1.96E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75923.t1	MTR_3g108770	69.014	142	16	2	1	114	1	142	3.88e-64	191
MS.gene75923.t1	MTR_1g045900	68.310	142	17	2	1	114	1	142	1.30e-63	190
MS.gene75923.t1	MTR_4g075190	62.937	143	23	3	1	114	1	142	1.41e-59	179
MS.gene75923.t1	MTR_5g075850	72.222	54	13	1	63	114	1	54	4.28e-22	82.4
MS.gene75923.t1	MTR_5g075820	68.889	45	14	0	70	114	110	154	1.13e-16	71.2
MS.gene75923.t1	MTR_1g054195	71.111	45	12	1	70	113	175	219	1.43e-16	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75923.t1	AT5G08290	66.901	142	19	2	1	114	1	142	1.08e-62	187
MS.gene75923.t1	AT5G08290	98.214	56	1	0	59	114	47	102	3.21e-36	119

Find 34 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTCTCGTTGTCATCCGTTT+TGG	0.320244	3.4:-94181317	MS.gene75923:CDS
GTACTTGGTGGAGTAATCTT+TGG	0.329915	3.4:+94180300	None:intergenic
GAAACAACAACAAGATCAAT+TGG	0.342414	3.4:-94180408	MS.gene75923:CDS
TTGAGAAGCAGAAACTATAT+TGG	0.344368	3.4:+94180936	None:intergenic
GCCTCACTTGCATTCGGGAT+GGG	0.362710	3.4:-94181372	MS.gene75923:CDS
TGAGAAGCAGAAACTATATT+GGG	0.366237	3.4:+94180937	None:intergenic
CATGCTGCCTCACTTGCATT+CGG	0.374586	3.4:-94181378	MS.gene75923:CDS
TTCGGGATGGGCTGTTGATC+AGG	0.384531	3.4:-94181360	MS.gene75923:CDS
TGATGTATTTACAGAGAAAC+AGG	0.387808	3.4:+94180901	None:intergenic
TACTTGGTGGAGTAATCTTT+GGG	0.403435	3.4:+94180301	None:intergenic
TTGGGCTATGAAGGACAAAC+AGG	0.451623	3.4:-94180389	MS.gene75923:CDS
CAAGATCAATTGGGCTATGA+AGG	0.451707	3.4:-94180398	MS.gene75923:CDS
TACATTCTAGTAACGGTACT+TGG	0.457670	3.4:+94180285	None:intergenic
TGCCTCACTTGCATTCGGGA+TGG	0.468994	3.4:-94181373	MS.gene75923:CDS
TGTTGTTGTTTCCAGTTCCA+AGG	0.469561	3.4:+94180419	None:intergenic
ATCATGATAGACCTTGGAAC+TGG	0.486256	3.4:-94180430	MS.gene75923:CDS
ATGCTGCCTCACTTGCATTC+GGG	0.490375	3.4:-94181377	MS.gene75923:CDS
AAACAACAACAAGATCAATT+GGG	0.514098	3.4:-94180407	MS.gene75923:CDS
TCTACAGAGGTGCAAGGAAG+GGG	0.542753	3.4:-94180342	MS.gene75923:CDS
CATCCCAATCGTGACCAAAA+CGG	0.547656	3.4:+94181303	None:intergenic
TGTCTACAGAGGTGCAAGGA+AGG	0.552161	3.4:-94180344	MS.gene75923:CDS
GTCTACAGAGGTGCAAGGAA+GGG	0.554185	3.4:-94180343	MS.gene75923:CDS
AAGCACATCATGATAGACCT+TGG	0.567283	3.4:-94180436	MS.gene75923:CDS
ATTCTAGTAACGGTACTTGG+TGG	0.586412	3.4:+94180288	None:intergenic
TTGGGATGAGACTTGTATGC+AGG	0.595858	3.4:-94181288	MS.gene75923:intron
TCAGGCAATCCTCGCTGAGG+AGG	0.599877	3.4:-94181342	MS.gene75923:CDS
AGAGGTGCAAGGAAGGGGCG+TGG	0.611606	3.4:-94180337	MS.gene75923:CDS
GCCCATCCCGAATGCAAGTG+AGG	0.615016	3.4:+94181371	None:intergenic
GAGAAGCAGAAACTATATTG+GGG	0.656478	3.4:+94180938	None:intergenic
AGATTGATACATTCTAGTAA+CGG	0.666479	3.4:+94180278	None:intergenic
TGATCAGGCAATCCTCGCTG+AGG	0.692917	3.4:-94181345	MS.gene75923:CDS
AGACTGTCTACAGAGGTGCA+AGG	0.715363	3.4:-94180348	MS.gene75923:CDS
ATCATTGAGACTGTCTACAG+AGG	0.717834	3.4:-94180355	MS.gene75923:CDS
CGAGACGTTCCTCCTCAGCG+AGG	0.755149	3.4:+94181333	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGCTTTTTTTTTTTTTTTT+TGG	-	chr3.4:94181066-94181085	MS.gene75923:intron	10.0%
!!	TTATGTCCTATATATATTAT+TGG	+	chr3.4:94180615-94180634	None:intergenic	15.0%
!!	AAACAGCCAATAATATATAT+AGG	-	chr3.4:94180606-94180625	MS.gene75923:intron	20.0%
!!	AGAGATAAAGTAATGAATTT+AGG	-	chr3.4:94180636-94180655	MS.gene75923:intron	20.0%
!!	ATCAACCTAAAATTAAGAAT+TGG	+	chr3.4:94180994-94181013	None:intergenic	20.0%
!!	GAGATAAAGTAATGAATTTA+GGG	-	chr3.4:94180637-94180656	MS.gene75923:intron	20.0%
!!!	TCTTAATTTTAGGTTGATAA+GGG	-	chr3.4:94180996-94181015	MS.gene75923:intron	20.0%
!!!	TTCTTAATTTTAGGTTGATA+AGG	-	chr3.4:94180995-94181014	MS.gene75923:intron	20.0%
!	AAACAACAACAAGATCAATT+GGG	-	chr3.4:94181270-94181289	MS.gene75923:intron	25.0%
!	ATAAAGTAATGAATTTAGGG+AGG	-	chr3.4:94180640-94180659	MS.gene75923:intron	25.0%
!	ATATCAAGAACTGCTTAATT+TGG	-	chr3.4:94180481-94180500	MS.gene75923:intron	25.0%
!	GCATGAAACAAATTTGAATT+TGG	+	chr3.4:94180685-94180704	None:intergenic	25.0%
!!	ATGCACCAATTCTTAATTTT+AGG	-	chr3.4:94180986-94181005	MS.gene75923:intron	25.0%
	AAACTATATTGGGGAAATAC+TGG	+	chr3.4:94180733-94180752	None:intergenic	30.0%
	CCATCACAATAACAATTTCA+TGG	-	chr3.4:94180886-94180905	MS.gene75923:intron	30.0%
	GAAACAACAACAAGATCAAT+TGG	-	chr3.4:94181269-94181288	MS.gene75923:intron	30.0%
	TCATACATAGTGTTAAAGTC+AGG	+	chr3.4:94181181-94181200	None:intergenic	30.0%
	TGAGAAGCAGAAACTATATT+GGG	+	chr3.4:94180743-94180762	None:intergenic	30.0%
	TGAGCGAAACTTGATTAATT+AGG	-	chr3.4:94181025-94181044	MS.gene75923:intron	30.0%
	TGATGTATTTACAGAGAAAC+AGG	+	chr3.4:94180779-94180798	None:intergenic	30.0%
	TTGAGAAGCAGAAACTATAT+TGG	+	chr3.4:94180744-94180763	None:intergenic	30.0%
!	CCATGAAATTGTTATTGTGA+TGG	+	chr3.4:94180889-94180908	None:intergenic	30.0%
	AAGAAGAACATAACTGTGGA+AGG	+	chr3.4:94181214-94181233	None:intergenic	35.0%
	AAGTAATGAATTTAGGGAGG+TGG	-	chr3.4:94180643-94180662	MS.gene75923:intron	35.0%
	AGAAGAACATAACTGTGGAA+GGG	+	chr3.4:94181213-94181232	None:intergenic	35.0%
	CCACAGTTATGTTCTTCTTT+AGG	-	chr3.4:94181215-94181234	MS.gene75923:intron	35.0%
	CCTAAAGAAGAACATAACTG+TGG	+	chr3.4:94181218-94181237	None:intergenic	35.0%
	GAGAAGCAGAAACTATATTG+GGG	+	chr3.4:94180742-94180761	None:intergenic	35.0%
	TATCATAATACCAATCGCAG+TGG	+	chr3.4:94180937-94180956	None:intergenic	35.0%
!	TACTTGGTGGAGTAATCTTT+GGG	+	chr3.4:94181379-94181398	None:intergenic	35.0%
!!!	TTTTTTGGTGTGTGTCAAGA+TGG	-	chr3.4:94181081-94181100	MS.gene75923:intron	35.0%
	AAGCACATCATGATAGACCT+TGG	-	chr3.4:94181241-94181260	MS.gene75923:intron	40.0%
	ATCATGATAGACCTTGGAAC+TGG	-	chr3.4:94181247-94181266	MS.gene75923:intron	40.0%
	ATCATTGAGACTGTCTACAG+AGG	-	chr3.4:94181322-94181341	MS.gene75923:CDS	40.0%
	CAAGATCAATTGGGCTATGA+AGG	-	chr3.4:94181279-94181298	MS.gene75923:intron	40.0%
!	CTTTGCTGTGATATACCTTG+TGG	-	chr3.4:94181141-94181160	MS.gene75923:intron	40.0%
!	GTACTTGGTGGAGTAATCTT+TGG	+	chr3.4:94181380-94181399	None:intergenic	40.0%
!!	TGTTGTTGTTTCCAGTTCCA+AGG	+	chr3.4:94181261-94181280	None:intergenic	40.0%
	ATACCTTGTGGACATCACAG+AGG	-	chr3.4:94181153-94181172	MS.gene75923:intron	45.0%
	CATCCCAATCGTGACCAAAA+CGG	+	chr3.4:94180377-94180396	None:intergenic	45.0%
	TTGGGATGAGACTTGTATGC+AGG	-	chr3.4:94180389-94180408	MS.gene75923:CDS	45.0%
	TTGGGCTATGAAGGACAAAC+AGG	-	chr3.4:94181288-94181307	MS.gene75923:intron	45.0%
!!	CATCCGTTTTGGTCACGATT+GGG	-	chr3.4:94180371-94180390	MS.gene75923:CDS	45.0%
!!	TCATCCGTTTTGGTCACGAT+TGG	-	chr3.4:94180370-94180389	MS.gene75923:CDS	45.0%
	ATGCTGCCTCACTTGCATTC+GGG	-	chr3.4:94180300-94180319	MS.gene75923:CDS	50.0%
	CATGCTGCCTCACTTGCATT+CGG	-	chr3.4:94180299-94180318	MS.gene75923:CDS	50.0%
	GGTGTGTGTCAAGATGGATG+AGG	-	chr3.4:94181087-94181106	MS.gene75923:intron	50.0%
!	AGACTGTCTACAGAGGTGCA+AGG	-	chr3.4:94181329-94181348	MS.gene75923:CDS	50.0%
!	CGTCTCGTTGTCATCCGTTT+TGG	-	chr3.4:94180360-94180379	MS.gene75923:CDS	50.0%
!	GTCTACAGAGGTGCAAGGAA+GGG	-	chr3.4:94181334-94181353	MS.gene75923:CDS	50.0%
!	TCTACAGAGGTGCAAGGAAG+GGG	-	chr3.4:94181335-94181354	MS.gene75923:CDS	50.0%
!	TGTCTACAGAGGTGCAAGGA+AGG	-	chr3.4:94181333-94181352	MS.gene75923:CDS	50.0%
!!	GATGGATGAGGTGCTGTCAT+CGG	-	chr3.4:94181099-94181118	MS.gene75923:intron	50.0%
	ACCAATCGCAGTGGTGTTCG+TGG	+	chr3.4:94180928-94180947	None:intergenic	55.0%
	ACCACGAACACCACTGCGAT+TGG	-	chr3.4:94180924-94180943	MS.gene75923:CDS	55.0%
	GCACCTCTGTGATGTCCACA+AGG	+	chr3.4:94181159-94181178	None:intergenic	55.0%
	GCCTCACTTGCATTCGGGAT+GGG	-	chr3.4:94180305-94180324	MS.gene75923:CDS	55.0%
	TGATCAGGCAATCCTCGCTG+AGG	-	chr3.4:94180332-94180351	MS.gene75923:CDS	55.0%
	TGCCTCACTTGCATTCGGGA+TGG	-	chr3.4:94180304-94180323	MS.gene75923:CDS	55.0%
!!	TTCGGGATGGGCTGTTGATC+AGG	-	chr3.4:94180317-94180336	MS.gene75923:CDS	55.0%
	GCCCATCCCGAATGCAAGTG+AGG	+	chr3.4:94180309-94180328	None:intergenic	60.0%
	TCAGGCAATCCTCGCTGAGG+AGG	-	chr3.4:94180335-94180354	MS.gene75923:CDS	60.0%
	CGAGACGTTCCTCCTCAGCG+AGG	+	chr3.4:94180347-94180366	None:intergenic	65.0%
!	AGAGGTGCAAGGAAGGGGCG+TGG	-	chr3.4:94181340-94181359	MS.gene75923:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	94180291	94181408	94180291	ID=MS.gene75923
chr3.4	mRNA	94180291	94181408	94180291	ID=MS.gene75923.t1;Parent=MS.gene75923
chr3.4	exon	94181289	94181408	94181289	ID=MS.gene75923.t1.exon1;Parent=MS.gene75923.t1
chr3.4	CDS	94181289	94181408	94181289	ID=cds.MS.gene75923.t1;Parent=MS.gene75923.t1
chr3.4	exon	94180913	94180965	94180913	ID=MS.gene75923.t1.exon2;Parent=MS.gene75923.t1
chr3.4	CDS	94180913	94180965	94180913	ID=cds.MS.gene75923.t1;Parent=MS.gene75923.t1
chr3.4	exon	94180291	94180462	94180291	ID=MS.gene75923.t1.exon3;Parent=MS.gene75923.t1
chr3.4	CDS	94180291	94180462	94180291	ID=cds.MS.gene75923.t1;Parent=MS.gene75923.t1

Gene Sequence

>MS.gene75923

ATGTCGTACATGCTGCCTCACTTGCATTCGGGATGGGCTGTTGATCAGGCAATCCTCGCTGAGGAGGAACGTCTCGTTGTCATCCGTTTTGGTCACGATTGGGATGAGACTTGTATGCAGGTACGTATAACATCAATCAATCAATCAATCAGTGTTTTATATTTTCATCTACTCATTCATTCACAGTAAAATATCAAGAACTGCTTAATTTGGAATGATGCTTTTTTATTAAGCAACAATTTCTTGTATGTATGTATGAGTTGAACAGTGCTATATAGCATGAATCTTTCTTGTATGAAAACAATACAGAACAGAAAACAGCCAATAATATATATAGGACATAAGAGAGATAAAGTAATGAATTTAGGGAGGTGGAGATTAATAAGATGCTCCAAATTCAAATTTGTTTCATGCTGATTTGTGTAGTTTTATTTCGTAACCAGTATTTCCCCAATATAGTTTCTGCTTCTCAACTTTTTCTGAAGCCTGTTTCTCTGTAAATACATCAACATCTCTACATGCTTAGAATATTTCTCTTTGCATATGAAGAATCATAAAGCTAATTCAAACTGAGTCATAGAGCTATTATCTTCAACCATCACAATAACAATTTCATGGATTTTACATGATGATACCACGAACACCACTGCGATTGGTATTATGATACATAATGTTTGTTTCTTTCAACAATCATCATGCACCAATTCTTAATTTTAGGTTGATAAGGGTTATATTGAGCGAAACTTGATTAATTAGGTTAGTGCTGCTGTTTGATTTGCTTTTTTTTTTTTTTTTTGGTGTGTGTCAAGATGGATGAGGTGCTGTCATCGGTTGCAGAGACAATAAAGAACTTTGCTGTGATATACCTTGTGGACATCACAGAGGTGCCTGACTTTAACACTATGTATGAGCTGTATGACCCTTCCACAGTTATGTTCTTCTTTAGGAACAAGCACATCATGATAGACCTTGGAACTGGAAACAACAACAAGATCAATTGGGCTATGAAGGACAAACAGGAGTTCATTGACATCATTGAGACTGTCTACAGAGGTGCAAGGAAGGGGCGTGGTCTTGTCATTGCTCCCAAAGATTACTCCACCAAGTACCGTTACTAG

Protein sequence

>MS.gene75923.t1

MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQYFPNIVSASQLFLKPVSLNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY