Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79466.t1 | KEH20991.1 | 97.3 | 640 | 12 | 1 | 65 | 699 | 66 | 705 | 0.00E+00 | 1240.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79466.t1 | F4JRS4 | 61.4 | 593 | 192 | 9 | 70 | 638 | 86 | 665 | 1.2e-197 | 691.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79466.t1 | A0A072TV01 | 97.3 | 640 | 12 | 1 | 65 | 699 | 66 | 705 | 0.0e+00 | 1240.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene79466 | 0.849373 | 3.37E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene79466 | 0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene049368 | MS.gene79466 | 0.82803 | 1.13E-54 | -1.69E-46 |
| MS.gene049983 | MS.gene79466 | 0.817107 | 3.91E-52 | -1.69E-46 |
| MS.gene050114 | MS.gene79466 | 0.804324 | 2.28E-49 | -1.69E-46 |
| MS.gene050439 | MS.gene79466 | 0.816527 | 5.28E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene79466 | 0.817356 | 3.44E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene79466 | 0.823738 | 1.18E-53 | -1.69E-46 |
| MS.gene051490 | MS.gene79466 | 0.811448 | 6.96E-51 | -1.69E-46 |
| MS.gene051676 | MS.gene79466 | 0.805605 | 1.23E-49 | -1.69E-46 |
| MS.gene051848 | MS.gene79466 | -0.812017 | 5.24E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene79466 | 0.850757 | 1.38E-60 | -1.69E-46 |
| MS.gene052307 | MS.gene79466 | 0.825703 | 4.06E-54 | -1.69E-46 |
| MS.gene052434 | MS.gene79466 | 0.821929 | 3.11E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene79466 | 0.826175 | 3.13E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene79466 | 0.800085 | 1.70E-48 | -1.69E-46 |
| MS.gene052609 | MS.gene79466 | 0.820947 | 5.24E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene79466 | 0.821847 | 3.25E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene79466 | 0.802691 | 4.97E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene79466 | 0.811804 | 5.83E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene79466 | 0.805089 | 1.58E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene79466 | 0.800345 | 1.51E-48 | -1.69E-46 |
| MS.gene054892 | MS.gene79466 | 0.805488 | 1.30E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene79466 | 0.804317 | 2.29E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene79466 | 0.821819 | 3.30E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene79466 | 0.854079 | 1.56E-61 | -1.69E-46 |
| MS.gene055314 | MS.gene79466 | 0.814826 | 1.26E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene79466 | 0.898897 | 3.38E-77 | -1.69E-46 |
| MS.gene055603 | MS.gene79466 | 0.818663 | 1.74E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene79466 | 0.846495 | 2.10E-59 | -1.69E-46 |
| MS.gene056045 | MS.gene79466 | 0.839366 | 1.65E-57 | -1.69E-46 |
| MS.gene056181 | MS.gene79466 | 0.829196 | 5.89E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene79466 | 0.814914 | 1.21E-51 | -1.69E-46 |
| MS.gene056509 | MS.gene79466 | 0.808418 | 3.13E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene79466 | 0.823147 | 1.62E-53 | -1.69E-46 |
| MS.gene056787 | MS.gene79466 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene056831 | MS.gene79466 | 0.847813 | 9.12E-60 | -1.69E-46 |
| MS.gene056986 | MS.gene79466 | 0.832461 | 9.33E-56 | -1.69E-46 |
| MS.gene057034 | MS.gene79466 | 0.846738 | 1.80E-59 | -1.69E-46 |
| MS.gene057102 | MS.gene79466 | 0.829043 | 6.42E-55 | -1.69E-46 |
| MS.gene057360 | MS.gene79466 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene79466 | 0.808665 | 2.77E-50 | -1.69E-46 |
| MS.gene058289 | MS.gene79466 | 0.809343 | 1.98E-50 | -1.69E-46 |
| MS.gene058668 | MS.gene79466 | 0.804672 | 1.93E-49 | -1.69E-46 |
| MS.gene058758 | MS.gene79466 | 0.802966 | 4.37E-49 | -1.69E-46 |
| MS.gene059144 | MS.gene79466 | 0.817116 | 3.90E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene79466 | 0.808476 | 3.04E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene79466 | 0.801642 | 8.18E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene79466 | 0.808174 | 3.53E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene79466 | 0.804548 | 2.05E-49 | -1.69E-46 |
| MS.gene059965 | MS.gene79466 | 0.810934 | 9.01E-51 | -1.69E-46 |
| MS.gene060435 | MS.gene79466 | 0.804432 | 2.17E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene79466 | 0.835902 | 1.28E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene79466 | 0.82722 | 1.76E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene79466 | 0.814909 | 1.21E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene79466 | 0.809959 | 1.46E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene79466 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene061160 | MS.gene79466 | 0.804468 | 2.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79466.t1 | MTR_8g096410 | 96.263 | 776 | 23 | 2 | 1 | 770 | 1 | 776 | 0.0 | 1474 |
| MS.gene79466.t1 | MTR_8g096410 | 96.015 | 778 | 23 | 3 | 1 | 770 | 1 | 778 | 0.0 | 1469 |
| MS.gene79466.t1 | MTR_8g096410 | 95.747 | 776 | 23 | 3 | 1 | 770 | 1 | 772 | 0.0 | 1462 |
| MS.gene79466.t1 | MTR_8g096410 | 93.258 | 801 | 23 | 3 | 1 | 770 | 1 | 801 | 0.0 | 1459 |
| MS.gene79466.t1 | MTR_3g077540 | 50.691 | 434 | 195 | 10 | 123 | 552 | 97 | 515 | 6.71e-136 | 418 |
| MS.gene79466.t1 | MTR_4g105530 | 43.551 | 535 | 258 | 18 | 122 | 639 | 90 | 597 | 6.65e-126 | 392 |
| MS.gene79466.t1 | MTR_8g076590 | 46.779 | 357 | 168 | 6 | 213 | 562 | 1 | 342 | 2.51e-108 | 340 |
| MS.gene79466.t1 | MTR_8g076580 | 61.728 | 81 | 31 | 0 | 123 | 203 | 55 | 135 | 8.53e-28 | 109 |
| MS.gene79466.t1 | MTR_5g024810 | 25.600 | 500 | 290 | 20 | 89 | 557 | 120 | 568 | 1.76e-22 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79466.t1 | AT5G50780 | 54.130 | 787 | 295 | 13 | 26 | 770 | 25 | 787 | 0.0 | 785 |
| MS.gene79466.t1 | AT5G50780 | 53.117 | 802 | 295 | 14 | 26 | 770 | 25 | 802 | 0.0 | 776 |
| MS.gene79466.t1 | AT4G24970 | 70.140 | 499 | 125 | 6 | 70 | 559 | 86 | 569 | 0.0 | 721 |
| MS.gene79466.t1 | AT5G50780 | 56.414 | 608 | 220 | 8 | 192 | 770 | 1 | 592 | 0.0 | 658 |
| MS.gene79466.t1 | AT5G13130 | 49.481 | 675 | 291 | 11 | 120 | 787 | 73 | 704 | 0.0 | 625 |
| MS.gene79466.t1 | AT5G13130 | 57.968 | 502 | 179 | 6 | 120 | 592 | 73 | 571 | 0.0 | 599 |
| MS.gene79466.t1 | AT4G36290 | 40.385 | 624 | 312 | 14 | 122 | 736 | 58 | 630 | 5.38e-144 | 439 |
| MS.gene79466.t1 | AT1G19100 | 49.885 | 435 | 202 | 7 | 122 | 556 | 86 | 504 | 1.39e-142 | 436 |
| MS.gene79466.t1 | AT4G36280 | 49.078 | 434 | 206 | 7 | 122 | 552 | 50 | 471 | 5.69e-141 | 431 |
| MS.gene79466.t1 | AT4G36270 | 43.681 | 364 | 153 | 9 | 212 | 554 | 1 | 333 | 1.01e-88 | 289 |
Find 175 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATCATATTGATGTTTC+AGG | 0.166512 | 4.1:+6346486 | MS.gene79466:CDS |
| TTAAGGGTTGTAAGCAGTTT+TGG | 0.256234 | 4.1:+6342426 | MS.gene79466:CDS |
| AATGTTAGCTTTAGTCTTAA+GGG | 0.266487 | 4.1:+6342410 | MS.gene79466:CDS |
| GTCTTTGCCTGCGCCGGAAT+GGG | 0.273806 | 4.1:+6342361 | MS.gene79466:CDS |
| GCTTCTGAGTGGTGCATCTA+TGG | 0.274397 | 4.1:+6350551 | MS.gene79466:CDS |
| AAATGTTAGCTTTAGTCTTA+AGG | 0.288360 | 4.1:+6342409 | MS.gene79466:CDS |
| GAGAAGAAAGAGGAAGAAAT+AGG | 0.291504 | 4.1:+6348699 | MS.gene79466:CDS |
| TCTTCTTTGTCTTCTTCTTT+CGG | 0.295574 | 4.1:-6342117 | None:intergenic |
| TACAAATGCAGAAAACTTAC+TGG | 0.338578 | 4.1:+6347168 | MS.gene79466:CDS |
| TGTCTTTGCCTGCGCCGGAA+TGG | 0.339469 | 4.1:+6342360 | MS.gene79466:CDS |
| GGAGCTAACTGCGATAAAAT+TGG | 0.343638 | 4.1:+6347292 | MS.gene79466:CDS |
| TCTAGTGCTGGAAAGTCATT+TGG | 0.345463 | 4.1:+6347703 | MS.gene79466:CDS |
| GGGTAATTATATACAATCTC+TGG | 0.353194 | 4.1:+6345654 | MS.gene79466:CDS |
| TTCAGCCATTCTGGAGGATC+TGG | 0.376798 | 4.1:+6346938 | MS.gene79466:intron |
| AGGTCGGCCTCATCAGAAAA+TGG | 0.381078 | 4.1:-6345446 | None:intergenic |
| GATTGAGATGATTCTAGAAC+AGG | 0.381894 | 4.1:-6347448 | None:intergenic |
| CCTTGTTAACTGCATTCATT+TGG | 0.383595 | 4.1:+6350529 | MS.gene79466:CDS |
| ATCTAAATCAACATCGGAAT+CGG | 0.397122 | 4.1:-6342192 | None:intergenic |
| CAGTTGGTATGGATCATGTC+AGG | 0.399359 | 4.1:+6343020 | MS.gene79466:intron |
| AAGTTTGAGAAAGAAGCTTC+AGG | 0.402778 | 4.1:+6349961 | MS.gene79466:CDS |
| GAAAGATAAGTGCAAATCTC+TGG | 0.403187 | 4.1:+6348749 | MS.gene79466:CDS |
| AGTTAAGAAGTGAGTAGAGT+TGG | 0.406267 | 4.1:-6345885 | None:intergenic |
| AGTGGTGGCCGTGGGGTCAT+AGG | 0.407424 | 4.1:+6346973 | MS.gene79466:CDS |
| AGAGATGATAAATCGGCTAT+TGG | 0.408814 | 4.1:-6348524 | None:intergenic |
| CTTTCCCACGAAGAATAATT+CGG | 0.409523 | 4.1:-6346245 | None:intergenic |
| AGCAAGATGGCAAATACAAT+TGG | 0.421977 | 4.1:+6344029 | MS.gene79466:CDS |
| CCTCAATCTGGAGTTGCTGA+TGG | 0.426807 | 4.1:+6346327 | MS.gene79466:CDS |
| TCCTGTTGTCAAGGAAAAGA+TGG | 0.427027 | 4.1:+6344198 | MS.gene79466:CDS |
| TAACCTTCTCAAGCAGCTCT+TGG | 0.427108 | 4.1:-6350454 | None:intergenic |
| CCAGCAGGAAGTGGTGGCCG+TGG | 0.429566 | 4.1:+6346964 | MS.gene79466:CDS |
| TGTAAAATCTGCAGTTGGTA+TGG | 0.432769 | 4.1:+6343009 | MS.gene79466:intron |
| TTCGATGTAGAAGCCCATTC+CGG | 0.434024 | 4.1:-6342374 | None:intergenic |
| CCCTAAATACCTGGCTAAGA+AGG | 0.442608 | 4.1:-6345466 | None:intergenic |
| ACAAGTCATAAGTGGGCTCT+TGG | 0.442932 | 4.1:+6343073 | MS.gene79466:CDS |
| CATTAGAATGTAGAAACTTC+GGG | 0.444015 | 4.1:-6343048 | None:intergenic |
| CCACGGCCACCACTTCCTGC+TGG | 0.444540 | 4.1:-6346964 | None:intergenic |
| GTCACGTACAGGCCTCAATC+TGG | 0.446312 | 4.1:+6346315 | MS.gene79466:CDS |
| TTCGACTATCACCCTTTGCT+TGG | 0.448771 | 4.1:-6347675 | None:intergenic |
| TTCCGATGTTGATTTAGATT+CGG | 0.450695 | 4.1:+6342196 | MS.gene79466:CDS |
| AACATCATATTGATGTTTCA+GGG | 0.452962 | 4.1:+6346487 | MS.gene79466:CDS |
| AAAAGAGAATCACGAGTTGA+AGG | 0.455380 | 4.1:+6348559 | MS.gene79466:CDS |
| CTGTTCTAGAGATGATAAAT+CGG | 0.455855 | 4.1:-6348531 | None:intergenic |
| CTGAGTTCGATGACCGAGTC+AGG | 0.456678 | 4.1:-6342164 | None:intergenic |
| TCCAGGATGCTGCTAGTAGA+AGG | 0.456812 | 4.1:+6343394 | MS.gene79466:CDS |
| AAGCCTTGCTTGTCATGGGC+AGG | 0.460834 | 4.1:-6347104 | None:intergenic |
| ATGATTGTTCTGATGAACTT+TGG | 0.471626 | 4.1:-6347603 | None:intergenic |
| AAAACAAGTACCATGAGGCT+TGG | 0.472120 | 4.1:+6344156 | MS.gene79466:CDS |
| AATACGTCAATGCATGTCAC+TGG | 0.472415 | 4.1:+6343992 | MS.gene79466:CDS |
| GTTAAGAAGTGAGTAGAGTT+GGG | 0.473939 | 4.1:-6345884 | None:intergenic |
| GCGTCGACGCCTGTCAATAA+TGG | 0.474195 | 4.1:+6342332 | MS.gene79466:CDS |
| AAGGAAGTCTTCAAGTCAAA+TGG | 0.478030 | 4.1:+6350487 | MS.gene79466:CDS |
| TTCAGATAACGGCGGTGGGA+TGG | 0.482461 | 4.1:+6343959 | MS.gene79466:intron |
| GGAGGATGACCAAGGTCAGC+TGG | 0.482744 | 4.1:+6345676 | MS.gene79466:CDS |
| GAGTAGAGTTGGGATAATCT+TGG | 0.482817 | 4.1:-6345874 | None:intergenic |
| GTTCAAAGCCTTGCTTGTCA+TGG | 0.485219 | 4.1:-6347109 | None:intergenic |
| TTCGGTTGTTGCTGCCGCTA+CGG | 0.486910 | 4.1:+6342214 | MS.gene79466:CDS |
| AGTTGCTGATGGGCTTCTAA+AGG | 0.487290 | 4.1:+6346338 | MS.gene79466:CDS |
| ACCTTCTACTAGCAGCATCC+TGG | 0.488031 | 4.1:-6343395 | None:intergenic |
| AGTAGAGTTGGGATAATCTT+GGG | 0.488491 | 4.1:-6345873 | None:intergenic |
| AGATACAGTGAACACAGAAA+TGG | 0.489155 | 4.1:+6347565 | MS.gene79466:CDS |
| TGGTGCATCTATGGTGGCAT+TGG | 0.489972 | 4.1:+6350560 | MS.gene79466:CDS |
| GGTGCATCTATGGTGGCATT+GGG | 0.494625 | 4.1:+6350561 | MS.gene79466:CDS |
| TCTTGCTCCTTCTCCTTCTC+CGG | 0.497329 | 4.1:+6342310 | MS.gene79466:CDS |
| ATGGGCAGGTTCAACAAAGT+TGG | 0.498194 | 4.1:-6347090 | None:intergenic |
| CAATGATATGATGTATTCTC+AGG | 0.500460 | 4.1:+6346290 | MS.gene79466:CDS |
| TAGAATCATCTCAATCGAAA+AGG | 0.500563 | 4.1:+6347455 | MS.gene79466:CDS |
| CTAGTGCTGGAAAGTCATTT+GGG | 0.501062 | 4.1:+6347704 | MS.gene79466:CDS |
| AAGCAAAACGGTAGTTCTGC+TGG | 0.501216 | 4.1:+6347337 | MS.gene79466:CDS |
| AGGAAAAGTTCCGCTACAAA+TGG | 0.501812 | 4.1:+6347475 | MS.gene79466:CDS |
| GGTTTCCGAATTATTCTTCG+TGG | 0.503402 | 4.1:+6346240 | MS.gene79466:CDS |
| TTCAAAGCCTTGCTTGTCAT+GGG | 0.504131 | 4.1:-6347108 | None:intergenic |
| CTTCATGTGATAGATGGAAA+TGG | 0.504512 | 4.1:+6344129 | MS.gene79466:intron |
| GTAGAAGCCCATTCCGGCGC+AGG | 0.505036 | 4.1:-6342368 | None:intergenic |
| CTCAATCTGGAGTTGCTGAT+GGG | 0.506171 | 4.1:+6346328 | MS.gene79466:CDS |
| GATGATTCTAGAACAGGATC+GGG | 0.507670 | 4.1:-6347442 | None:intergenic |
| GCATTAGAATGTAGAAACTT+CGG | 0.511635 | 4.1:-6343049 | None:intergenic |
| ATTTGTATTTACAGGTGTGT+TGG | 0.512149 | 4.1:+6347064 | MS.gene79466:intron |
| GACAAAACACCATTATTGAC+AGG | 0.513798 | 4.1:-6342341 | None:intergenic |
| TTTCAGGATATTCAAATACA+AGG | 0.515733 | 4.1:+6345818 | MS.gene79466:intron |
| CGAGTAAGAAAGATGGCTCC+AGG | 0.517005 | 4.1:+6343377 | MS.gene79466:CDS |
| CTTATTTCAGATAACGGCGG+TGG | 0.518759 | 4.1:+6343954 | MS.gene79466:intron |
| GTTGGACTTCAGGTTTGTAA+TGG | 0.522260 | 4.1:+6343325 | MS.gene79466:intron |
| CAGCAGGAAGTGGTGGCCGT+GGG | 0.523090 | 4.1:+6346965 | MS.gene79466:CDS |
| TTTGGACAATGCATTGGATG+AGG | 0.526190 | 4.1:+6343225 | MS.gene79466:CDS |
| CGTGCCTTCGAATCTTCAAC+CGG | 0.526751 | 4.1:+6342479 | MS.gene79466:CDS |
| CATATCTACATTTACATATG+TGG | 0.529551 | 4.1:-6343350 | None:intergenic |
| GAAGATTCGAAGGCACGAGC+AGG | 0.530273 | 4.1:-6342473 | None:intergenic |
| GTCAAGGAAAAGATGGGAAG+AGG | 0.531099 | 4.1:+6344205 | MS.gene79466:CDS |
| TTCTAGATTAGAGAAGAAAG+AGG | 0.532224 | 4.1:+6348689 | MS.gene79466:intron |
| AGTCGAAATATATCTAGTGC+TGG | 0.532649 | 4.1:+6347691 | MS.gene79466:CDS |
| AGATGATTCTAGAACAGGAT+CGG | 0.535794 | 4.1:-6347443 | None:intergenic |
| ATCTGGAATCCAGCAGGAAG+TGG | 0.538539 | 4.1:+6346955 | MS.gene79466:CDS |
| TGAGGCCGACCTTCTTAGCC+AGG | 0.543176 | 4.1:+6345457 | MS.gene79466:CDS |
| TCGATGACCGAGTCAGGTAT+CGG | 0.544821 | 4.1:-6342158 | None:intergenic |
| TCGTCAAAGAACCAAGCAAA+GGG | 0.546122 | 4.1:+6347664 | MS.gene79466:CDS |
| GGTAATTATATACAATCTCT+GGG | 0.546744 | 4.1:+6345655 | MS.gene79466:CDS |
| AATTGAGTTCCAGCTGACCT+TGG | 0.548168 | 4.1:-6345685 | None:intergenic |
| TGCGAACTTCTCTAGATGAC+TGG | 0.548230 | 4.1:+6345390 | MS.gene79466:CDS |
| AATGGGCTTCTACATCGAAC+CGG | 0.552678 | 4.1:+6342378 | MS.gene79466:CDS |
| CTAATGCCACAAGTCATAAG+TGG | 0.555360 | 4.1:+6343065 | MS.gene79466:CDS |
| ACCGAGTCAGGTATCGGAGC+GGG | 0.555423 | 4.1:-6342152 | None:intergenic |
| AGTGCAAATCTCTGGAAACC+CGG | 0.555432 | 4.1:+6348757 | MS.gene79466:CDS |
| TCATAAGAATCGACTCATCA+AGG | 0.557946 | 4.1:+6346521 | MS.gene79466:CDS |
| CTTCTCAAGCAGCTCTTGGA+TGG | 0.558462 | 4.1:-6350450 | None:intergenic |
| GTCGACGCCGGAGAAGGAGA+AGG | 0.558649 | 4.1:-6342317 | None:intergenic |
| GTTTCCGAATTATTCTTCGT+GGG | 0.563425 | 4.1:+6346241 | MS.gene79466:CDS |
| AAATCCTTCCAAGAAAAGAA+AGG | 0.565781 | 4.1:+6342241 | MS.gene79466:CDS |
| CCTGTTGTCAAGGAAAAGAT+GGG | 0.569016 | 4.1:+6344199 | MS.gene79466:CDS |
| GGATTCCAGATCCTCCAGAA+TGG | 0.570173 | 4.1:-6346943 | None:intergenic |
| TGGAGGATCTGGAATCCAGC+AGG | 0.570456 | 4.1:+6346949 | MS.gene79466:CDS |
| AGTCATAAGTGGGCTCTTGG+AGG | 0.570495 | 4.1:+6343076 | MS.gene79466:CDS |
| CCAAATGAATGCAGTTAACA+AGG | 0.571108 | 4.1:-6350529 | None:intergenic |
| AGAAGGTTAGATCACTTGAA+AGG | 0.571506 | 4.1:+6350468 | MS.gene79466:CDS |
| ATGGTTGCTACTGTTACTAT+CGG | 0.572756 | 4.1:+6346447 | MS.gene79466:CDS |
| TGATGTCTTCTCTGAAGAGA+GGG | 0.574275 | 4.1:+6349916 | MS.gene79466:CDS |
| ACAGGCGTCGACGCCGGAGA+AGG | 0.574660 | 4.1:-6342323 | None:intergenic |
| CATCCAAGAGCTGCTTGAGA+AGG | 0.576882 | 4.1:+6350451 | MS.gene79466:CDS |
| TACATCTGCTCCAAGCCTCA+TGG | 0.580775 | 4.1:-6344166 | None:intergenic |
| TGTTACTATCGGATTTGTCA+AGG | 0.580977 | 4.1:+6346458 | MS.gene79466:CDS |
| ATTCTCAGGAGGTCACGTAC+AGG | 0.582301 | 4.1:+6346304 | MS.gene79466:CDS |
| CTCGTCAAAGAACCAAGCAA+AGG | 0.584131 | 4.1:+6347663 | MS.gene79466:CDS |
| ATCAGGCCTACACGGAGCAT+AGG | 0.585217 | 4.1:+6344916 | MS.gene79466:intron |
| AATCGAAATAGAAAGCAAAA+CGG | 0.586695 | 4.1:+6347325 | MS.gene79466:CDS |
| CCATCAGCAACTCCAGATTG+AGG | 0.586958 | 4.1:-6346327 | None:intergenic |
| TTGATGTCTTCTCTGAAGAG+AGG | 0.587046 | 4.1:+6349915 | MS.gene79466:CDS |
| ATATAATTACCCGTGTACCT+TGG | 0.592587 | 4.1:-6345643 | None:intergenic |
| TGCTGGAAAGTCATTTGGGA+AGG | 0.593569 | 4.1:+6347708 | MS.gene79466:CDS |
| TGGAAAGCCGGTGATTATGG+TGG | 0.594419 | 4.1:+6342446 | MS.gene79466:CDS |
| AGAATCACGAGTTGAAGGAG+AGG | 0.594654 | 4.1:+6348564 | MS.gene79466:CDS |
| AACTTATCGACATTCACTGA+GGG | 0.596733 | 4.1:+6345904 | MS.gene79466:CDS |
| AAAAGAAAGGTTAATGACAA+AGG | 0.596916 | 4.1:+6342254 | MS.gene79466:CDS |
| TCTGAGTGGTGCATCTATGG+TGG | 0.598619 | 4.1:+6350554 | MS.gene79466:CDS |
| GGAGAAGGAGAAGGAGCAAG+AGG | 0.599429 | 4.1:-6342308 | None:intergenic |
| GTGCAAATCTCTGGAAACCC+GGG | 0.600738 | 4.1:+6348758 | MS.gene79466:CDS |
| GGAAGACATTGTAGTGCCCA+TGG | 0.602512 | 4.1:+6344975 | MS.gene79466:CDS |
| AAGAGCCCACTTATGACTTG+TGG | 0.607772 | 4.1:-6343071 | None:intergenic |
| ATACGTCAATGCATGTCACT+GGG | 0.610043 | 4.1:+6343993 | MS.gene79466:CDS |
| AGTTGGTATGGATCATGTCA+GGG | 0.610107 | 4.1:+6343021 | MS.gene79466:intron |
| GGTTGTTGCTGCCGCTACGG+CGG | 0.614002 | 4.1:+6342217 | MS.gene79466:CDS |
| TGGAATCCAGCAGGAAGTGG+TGG | 0.614206 | 4.1:+6346958 | MS.gene79466:CDS |
| GCATTCATTTGGCTTCTGAG+TGG | 0.616440 | 4.1:+6350540 | MS.gene79466:CDS |
| AGGAGAACCACCATAATCAC+CGG | 0.616981 | 4.1:-6342453 | None:intergenic |
| TTATTTCAGATAACGGCGGT+GGG | 0.618674 | 4.1:+6343955 | MS.gene79466:intron |
| ATGCTTGCGAGTAAGAAAGA+TGG | 0.618736 | 4.1:+6343370 | MS.gene79466:CDS |
| AAGTTCCGCTACAAATGGCA+AGG | 0.619802 | 4.1:+6347480 | MS.gene79466:CDS |
| AAATACCTGGCTAAGAAGGT+CGG | 0.620846 | 4.1:-6345462 | None:intergenic |
| ATGCCCCACCTATGACCCCA+CGG | 0.621901 | 4.1:-6346981 | None:intergenic |
| AACTTTGGTTCACTGATGAG+CGG | 0.622636 | 4.1:-6347588 | None:intergenic |
| ATTGTGTTCTCCTGTTGTCA+AGG | 0.626229 | 4.1:+6344189 | MS.gene79466:CDS |
| GAACCTGCCCATGACAAGCA+AGG | 0.627707 | 4.1:+6347101 | MS.gene79466:CDS |
| ACCCGCTCCGATACCTGACT+CGG | 0.627883 | 4.1:+6342151 | MS.gene79466:CDS |
| CTTACCGGTTGAAGATTCGA+AGG | 0.629128 | 4.1:-6342483 | None:intergenic |
| TGTTGCCTTGCCATTTGTAG+CGG | 0.630952 | 4.1:-6347485 | None:intergenic |
| TAATGCCACAAGTCATAAGT+GGG | 0.632893 | 4.1:+6343066 | MS.gene79466:CDS |
| TTATTGACAGGCGTCGACGC+CGG | 0.632994 | 4.1:-6342329 | None:intergenic |
| AACCGAATCTAAATCAACAT+CGG | 0.635484 | 4.1:-6342198 | None:intergenic |
| TTATATCTGAGGATTCCTCA+AGG | 0.638211 | 4.1:+6346219 | MS.gene79466:CDS |
| TGATATGATGTATTCTCAGG+AGG | 0.641459 | 4.1:+6346293 | MS.gene79466:CDS |
| AGAAAGGTTAATGACAAAGG+TGG | 0.646834 | 4.1:+6342257 | MS.gene79466:CDS |
| AGCAGGAAGTGGTGGCCGTG+GGG | 0.647388 | 4.1:+6346966 | MS.gene79466:CDS |
| AGAATAATTCGGAAACCTTG+AGG | 0.651583 | 4.1:-6346234 | None:intergenic |
| TACACAAGGATCTAACCCAA+AGG | 0.660630 | 4.1:+6347735 | MS.gene79466:CDS |
| ATATTCTGAGAAAAGCAAGA+TGG | 0.668137 | 4.1:+6344016 | MS.gene79466:CDS |
| ATGTTGAAACAGTAGTTCAG+TGG | 0.675772 | 4.1:+6345420 | MS.gene79466:CDS |
| AGCAAGCCTATGCTCCGTGT+AGG | 0.693402 | 4.1:-6344922 | None:intergenic |
| AAAGTTAGATGACTTGAACA+AGG | 0.700282 | 4.1:+6349877 | MS.gene79466:CDS |
| AATCTCTGGGAGGATGACCA+AGG | 0.701386 | 4.1:+6345668 | MS.gene79466:CDS |
| TAACTTATCGACATTCACTG+AGG | 0.713108 | 4.1:+6345903 | MS.gene79466:CDS |
| ATTCTTATTTCAGATAACGG+CGG | 0.713205 | 4.1:+6343951 | MS.gene79466:intron |
| GCTTTAAAACAAGTACCATG+AGG | 0.717774 | 4.1:+6344151 | MS.gene79466:CDS |
| TTTGGGAAGGAGTCTACACA+AGG | 0.723742 | 4.1:+6347721 | MS.gene79466:CDS |
| AGTATTGCAAGCTTTAGACG+AGG | 0.724992 | 4.1:+6348725 | MS.gene79466:CDS |
| GACCGAGTCAGGTATCGGAG+CGG | 0.730390 | 4.1:-6342153 | None:intergenic |
| AGAGGACTGAGAAATCCCGC+CGG | 0.733991 | 4.1:-6342290 | None:intergenic |
| AATTATATACAATCTCTGGG+AGG | 0.747675 | 4.1:+6345658 | MS.gene79466:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6342071 | 6350590 | 6342071 | ID=MS.gene79466 |
| chr4.1 | mRNA | 6342071 | 6350590 | 6342071 | ID=MS.gene79466.t1;Parent=MS.gene79466 |
| chr4.1 | exon | 6342071 | 6342500 | 6342071 | ID=MS.gene79466.t1.exon1;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6342071 | 6342500 | 6342071 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6343023 | 6343097 | 6343023 | ID=MS.gene79466.t1.exon2;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6343023 | 6343097 | 6343023 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6343212 | 6343246 | 6343212 | ID=MS.gene79466.t1.exon3;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6343212 | 6343246 | 6343212 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6343337 | 6343415 | 6343337 | ID=MS.gene79466.t1.exon4;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6343337 | 6343415 | 6343337 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6343964 | 6344056 | 6343964 | ID=MS.gene79466.t1.exon5;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6343964 | 6344056 | 6343964 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6344142 | 6344226 | 6344142 | ID=MS.gene79466.t1.exon6;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6344142 | 6344226 | 6344142 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6344921 | 6344996 | 6344921 | ID=MS.gene79466.t1.exon7;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6344921 | 6344996 | 6344921 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6345350 | 6345478 | 6345350 | ID=MS.gene79466.t1.exon8;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6345350 | 6345478 | 6345350 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6345626 | 6345724 | 6345626 | ID=MS.gene79466.t1.exon9;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6345626 | 6345724 | 6345626 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6345824 | 6345925 | 6345824 | ID=MS.gene79466.t1.exon10;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6345824 | 6345925 | 6345824 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6346204 | 6346368 | 6346204 | ID=MS.gene79466.t1.exon11;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6346204 | 6346368 | 6346204 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6346447 | 6346542 | 6346447 | ID=MS.gene79466.t1.exon12;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6346447 | 6346542 | 6346447 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6346943 | 6346994 | 6346943 | ID=MS.gene79466.t1.exon13;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6346943 | 6346994 | 6346943 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6347078 | 6347189 | 6347078 | ID=MS.gene79466.t1.exon14;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6347078 | 6347189 | 6347078 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6347291 | 6347358 | 6347291 | ID=MS.gene79466.t1.exon15;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6347291 | 6347358 | 6347291 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6347434 | 6347756 | 6347434 | ID=MS.gene79466.t1.exon16;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6347434 | 6347756 | 6347434 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6348503 | 6348585 | 6348503 | ID=MS.gene79466.t1.exon17;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6348503 | 6348585 | 6348503 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6348695 | 6348779 | 6348695 | ID=MS.gene79466.t1.exon18;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6348695 | 6348779 | 6348695 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6349860 | 6349982 | 6349860 | ID=MS.gene79466.t1.exon19;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6349860 | 6349982 | 6349860 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| chr4.1 | exon | 6350441 | 6350590 | 6350441 | ID=MS.gene79466.t1.exon20;Parent=MS.gene79466.t1 |
| chr4.1 | CDS | 6350441 | 6350590 | 6350441 | ID=cds.MS.gene79466.t1;Parent=MS.gene79466.t1 |
| Gene Sequence |
| Protein sequence |
