Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85726.t1 | KEH25858.1 | 95.8 | 383 | 13 | 1 | 1 | 383 | 1 | 380 | 4.50E-198 | 700.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85726.t1 | A0A072U9Y9 | 95.8 | 383 | 13 | 1 | 1 | 383 | 1 | 380 | 3.3e-198 | 700.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene85726.t1 | TF | Trihelix |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene85726 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene85726 | 0.806056 | 9.90E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene85726 | 0.823533 | 1.32E-53 | -1.69E-46 |
| MS.gene050105 | MS.gene85726 | 0.842027 | 3.32E-58 | -1.69E-46 |
| MS.gene050226 | MS.gene85726 | 0.807082 | 6.01E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene85726 | 0.823109 | 1.65E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene85726 | 0.830765 | 2.44E-55 | -1.69E-46 |
| MS.gene050442 | MS.gene85726 | 0.807714 | 4.42E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene85726 | 0.816067 | 6.69E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene85726 | 0.810395 | 1.18E-50 | -1.69E-46 |
| MS.gene050562 | MS.gene85726 | 0.800827 | 1.20E-48 | -1.69E-46 |
| MS.gene050680 | MS.gene85726 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene85726 | 0.842413 | 2.63E-58 | -1.69E-46 |
| MS.gene050780 | MS.gene85726 | 0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene85726 | 0.818669 | 1.74E-52 | -1.69E-46 |
| MS.gene050987 | MS.gene85726 | 0.81023 | 1.28E-50 | -1.69E-46 |
| MS.gene051122 | MS.gene85726 | 0.803237 | 3.84E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene85726 | 0.807054 | 6.10E-50 | -1.69E-46 |
| MS.gene051926 | MS.gene85726 | -0.825924 | 3.59E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene85726 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene85726 | 0.863161 | 3.03E-64 | -1.69E-46 |
| MS.gene052190 | MS.gene85726 | 0.832287 | 1.03E-55 | -1.69E-46 |
| MS.gene052333 | MS.gene85726 | 0.845481 | 3.95E-59 | -1.69E-46 |
| MS.gene052358 | MS.gene85726 | -0.83295 | 7.05E-56 | -1.69E-46 |
| MS.gene052389 | MS.gene85726 | 0.818028 | 2.43E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene85726 | 0.811333 | 7.38E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene85726 | 0.825365 | 4.88E-54 | -1.69E-46 |
| MS.gene052954 | MS.gene85726 | 0.808714 | 2.70E-50 | -1.69E-46 |
| MS.gene052965 | MS.gene85726 | -0.805751 | 1.15E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene85726 | 0.814836 | 1.26E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene85726 | 0.820859 | 5.49E-53 | -1.69E-46 |
| MS.gene05414 | MS.gene85726 | 0.803056 | 4.18E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene85726 | 0.835542 | 1.58E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene85726 | -0.835175 | 1.96E-56 | -1.69E-46 |
| MS.gene054405 | MS.gene85726 | 0.813114 | 3.02E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene85726 | 0.822061 | 2.90E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene85726 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene054657 | MS.gene85726 | 0.805933 | 1.05E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene85726 | 0.805407 | 1.35E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene85726 | 0.807017 | 6.21E-50 | -1.69E-46 |
| MS.gene055225 | MS.gene85726 | 0.815526 | 8.84E-52 | -1.69E-46 |
| MS.gene055230 | MS.gene85726 | 0.816312 | 5.90E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene85726 | 0.84063 | 7.75E-58 | -1.69E-46 |
| MS.gene055818 | MS.gene85726 | 0.821377 | 4.17E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene85726 | 0.809223 | 2.10E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene85726 | 0.850114 | 2.09E-60 | -1.69E-46 |
| MS.gene056779 | MS.gene85726 | 0.835953 | 1.24E-56 | -1.69E-46 |
| MS.gene057026 | MS.gene85726 | 0.833423 | 5.38E-56 | -1.69E-46 |
| MS.gene057060 | MS.gene85726 | 0.82191 | 3.14E-53 | -1.69E-46 |
| MS.gene057156 | MS.gene85726 | -0.837341 | 5.50E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene85726 | 0.811643 | 6.32E-51 | -1.69E-46 |
| MS.gene05761 | MS.gene85726 | 0.840578 | 7.99E-58 | -1.69E-46 |
| MS.gene058625 | MS.gene85726 | 0.825717 | 4.03E-54 | -1.69E-46 |
| MS.gene059161 | MS.gene85726 | 0.800536 | 1.38E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene85726 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene059710 | MS.gene85726 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene85726 | 0.816558 | 5.20E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene85726 | 0.846921 | 1.60E-59 | -1.69E-46 |
| MS.gene060572 | MS.gene85726 | 0.806917 | 6.52E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene85726 | 0.82166 | 3.59E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene85726 | 0.821203 | 4.57E-53 | -1.69E-46 |
| MS.gene060840 | MS.gene85726 | 0.801591 | 8.38E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene85726 | 0.82777 | 1.30E-54 | -1.69E-46 |
| MS.gene061040 | MS.gene85726 | -0.853966 | 1.68E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85726.t1 | MTR_6g035315 | 96.019 | 427 | 17 | 0 | 25 | 451 | 22 | 448 | 0.0 | 748 |
| MS.gene85726.t1 | MTR_1g098920 | 71.096 | 429 | 111 | 6 | 24 | 445 | 23 | 445 | 0.0 | 589 |
| MS.gene85726.t1 | MTR_2g098080 | 41.928 | 415 | 209 | 6 | 37 | 444 | 22 | 411 | 6.01e-91 | 283 |
| MS.gene85726.t1 | MTR_7g103390 | 50.000 | 244 | 101 | 6 | 11 | 248 | 1 | 229 | 3.32e-66 | 219 |
| MS.gene85726.t1 | MTR_1g061640 | 62.420 | 157 | 56 | 3 | 93 | 248 | 95 | 249 | 5.78e-58 | 198 |
| MS.gene85726.t1 | MTR_1g061640 | 49.333 | 75 | 38 | 0 | 371 | 445 | 406 | 480 | 5.72e-18 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85726.t1 | AT1G21200 | 61.707 | 457 | 147 | 10 | 1 | 446 | 1 | 440 | 8.94e-179 | 507 |
| MS.gene85726.t1 | AT1G21200 | 61.707 | 457 | 147 | 10 | 1 | 446 | 1 | 440 | 8.94e-179 | 507 |
| MS.gene85726.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 4.45e-126 | 371 |
| MS.gene85726.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 4.45e-126 | 371 |
| MS.gene85726.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 4.45e-126 | 371 |
| MS.gene85726.t1 | AT3G10040 | 44.622 | 251 | 109 | 8 | 21 | 252 | 7 | 246 | 5.30e-46 | 165 |
Find 114 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGCAGTGTTGCAGAAAA+AGG | 0.201562 | 6.1:+16614543 | MS.gene85726:CDS |
| AGAAGGAAGTAGAGGGAAAA+AGG | 0.214962 | 6.1:+16614405 | MS.gene85726:CDS |
| AATGCAATCTATTACAATTA+TGG | 0.268261 | 6.1:-16614822 | None:intergenic |
| CATGAAGATGCTACTACTTT+TGG | 0.268448 | 6.1:+16615078 | MS.gene85726:CDS |
| GGTGATAGTCGAGGAATCTT+TGG | 0.290899 | 6.1:+16615012 | MS.gene85726:CDS |
| AATTCAACGATCTTAATAAA+AGG | 0.306147 | 6.1:+16614638 | MS.gene85726:CDS |
| ATAACGGCGGTGTCTTATAT+TGG | 0.308369 | 6.1:+16614478 | MS.gene85726:CDS |
| TATCATGATCATCTCTATTT+CGG | 0.312915 | 6.1:-16614888 | None:intergenic |
| ATCTTAATAAAAGGTATAAA+AGG | 0.318059 | 6.1:+16614647 | MS.gene85726:CDS |
| TGGAAATTCTTTAAATTGTC+AGG | 0.326876 | 6.1:+16615098 | MS.gene85726:CDS |
| GAAATCTATTTCCAAGGTTA+TGG | 0.330531 | 6.1:+16614573 | MS.gene85726:CDS |
| CAAAAGTAGTAGCATCTTCA+TGG | 0.344743 | 6.1:-16615077 | None:intergenic |
| CTGTCAAGTTGTTGAAAATC+CGG | 0.348018 | 6.1:+16614699 | MS.gene85726:CDS |
| GTGATAGTCGAGGAATCTTT+GGG | 0.360170 | 6.1:+16615013 | MS.gene85726:CDS |
| TGACGAGGATGATCATGATA+TGG | 0.392416 | 6.1:+16614945 | MS.gene85726:CDS |
| TTCCAAGGTTATGGCTGAAA+GGG | 0.411155 | 6.1:+16614582 | MS.gene85726:CDS |
| CGGCGGCACCAGCTTTAACT+AGG | 0.419109 | 6.1:+16615419 | MS.gene85726:CDS |
| GTCGATGAACGAGTTTAGTC+AGG | 0.421399 | 6.1:+16614315 | MS.gene85726:CDS |
| TTCAACGTCCCTATTGTAAG+AGG | 0.429215 | 6.1:-16614274 | None:intergenic |
| CAAGGTGATGGTGGTGGTGG+TGG | 0.436461 | 6.1:+16614118 | MS.gene85726:CDS |
| AGATGGAAATCAAGCTTTAC+CGG | 0.441260 | 6.1:+16615164 | MS.gene85726:CDS |
| GTTCAAGGTGATGGTGGTGG+TGG | 0.446247 | 6.1:+16614115 | MS.gene85726:CDS |
| TGCAAGGACCGCTGCAATGC+AGG | 0.447004 | 6.1:-16614856 | None:intergenic |
| GTGCTTTCTTCGATTAATGA+AGG | 0.476946 | 6.1:+16614247 | MS.gene85726:CDS |
| TAGTAGCATCTTCATGGCCT+TGG | 0.479201 | 6.1:-16615071 | None:intergenic |
| GGTAAAGCTTGATTTCCATC+TGG | 0.481200 | 6.1:-16615162 | None:intergenic |
| AACTCATCCGTGTCAAGGGC+CGG | 0.484357 | 6.1:+16614222 | MS.gene85726:CDS |
| ACAGATTCAGATTGAGATGA+TGG | 0.485981 | 6.1:+16615260 | MS.gene85726:CDS |
| TGTTGAAAATCCGGCTTTGT+TGG | 0.486943 | 6.1:+16614708 | MS.gene85726:CDS |
| TCTCAAGGAGGTATAGTTCA+AGG | 0.489422 | 6.1:+16614100 | MS.gene85726:CDS |
| CGCCTTGGCAGCGTGTGAAG+TGG | 0.492977 | 6.1:+16614431 | MS.gene85726:CDS |
| GGGAGTTCTGAAGGTGGTAG+TGG | 0.493151 | 6.1:+16614508 | MS.gene85726:CDS |
| CAGCTTGGTTACAGAAGCAA+TGG | 0.506444 | 6.1:+16615199 | MS.gene85726:CDS |
| AAAAGGCTTAACGATATGCT+TGG | 0.506945 | 6.1:+16614664 | MS.gene85726:CDS |
| CGATGAACTCGCATCGATCC+CGG | 0.508975 | 6.1:-16614163 | None:intergenic |
| CGCTGCAATGCAGGATCATG+CGG | 0.517016 | 6.1:-16614847 | None:intergenic |
| GTGTCTTATATTGGCGAGGA+CGG | 0.520156 | 6.1:+16614487 | MS.gene85726:CDS |
| ACCCCTTTCAGCCATAACCT+TGG | 0.524702 | 6.1:-16614584 | None:intergenic |
| TTTCCAAGGTTATGGCTGAA+AGG | 0.525735 | 6.1:+16614581 | MS.gene85726:CDS |
| CTGAAGGTGGTAGTGGTGGA+AGG | 0.527560 | 6.1:+16614515 | MS.gene85726:CDS |
| CATAAAAGTTCGTATCCTCA+TGG | 0.535597 | 6.1:+16615126 | MS.gene85726:CDS |
| CTCGTCATGATCATCATGAT+AGG | 0.537593 | 6.1:-16614929 | None:intergenic |
| GAGGAAGATGAGCCGGAGGA+AGG | 0.539815 | 6.1:+16614364 | MS.gene85726:CDS |
| AGCTTGGTTACAGAAGCAAT+GGG | 0.542768 | 6.1:+16615200 | MS.gene85726:CDS |
| GAGCCGGAGGAAGGTGGTGA+TGG | 0.542968 | 6.1:+16614373 | MS.gene85726:CDS |
| GGCTTAACGATATGCTTGGA+AGG | 0.546544 | 6.1:+16614668 | MS.gene85726:CDS |
| GAACTGAAGCGAAAGGAGAT+CGG | 0.553087 | 6.1:+16615399 | MS.gene85726:CDS |
| CATCAAACTCATCCGTGTCA+AGG | 0.557158 | 6.1:+16614217 | MS.gene85726:CDS |
| TGTCTTATATTGGCGAGGAC+GGG | 0.557593 | 6.1:+16614488 | MS.gene85726:CDS |
| GGGGAGTGAGGAAGATGAGC+CGG | 0.557735 | 6.1:+16614357 | MS.gene85726:CDS |
| CGGAAAACATGAAAGCAGCT+TGG | 0.558783 | 6.1:+16615184 | MS.gene85726:CDS |
| ATCAAACTCATCCGTGTCAA+GGG | 0.560403 | 6.1:+16614218 | MS.gene85726:CDS |
| GTATCTTAACGAGAAAGAAA+AGG | 0.560500 | 6.1:+16614741 | MS.gene85726:CDS |
| GACACGGATGAGTTTGATGT+TGG | 0.561258 | 6.1:-16614213 | None:intergenic |
| GGCGAGGACGGGAGTTCTGA+AGG | 0.563055 | 6.1:+16614499 | MS.gene85726:CDS |
| ATAGTTCAAGGTGATGGTGG+TGG | 0.564193 | 6.1:+16614112 | MS.gene85726:CDS |
| AGAGTTGGAGAAGTTCAAGC+TGG | 0.565535 | 6.1:+16615335 | MS.gene85726:CDS |
| TGATGTTGGTTCATCGTATG+CGG | 0.567222 | 6.1:-16614199 | None:intergenic |
| GGATGATCATGATATGGAAA+CGG | 0.567609 | 6.1:+16614951 | MS.gene85726:CDS |
| TAAGTGGAAATCTATTTCCA+AGG | 0.570877 | 6.1:+16614567 | MS.gene85726:CDS |
| GCTTGAACTTCTCCAACTCT+CGG | 0.574294 | 6.1:-16615332 | None:intergenic |
| TCAATTACATCCAACAAAGC+CGG | 0.575896 | 6.1:-16614718 | None:intergenic |
| GGTATAGTTCAAGGTGATGG+TGG | 0.576507 | 6.1:+16614109 | MS.gene85726:CDS |
| AGCACCGCCGGCCCTTGACA+CGG | 0.577678 | 6.1:-16614229 | None:intergenic |
| GCTTAACGATATGCTTGGAA+GGG | 0.580590 | 6.1:+16614669 | MS.gene85726:CDS |
| GGAGGTATAGTTCAAGGTGA+TGG | 0.582990 | 6.1:+16614106 | MS.gene85726:CDS |
| TGCTTTAGAACTGAAGCGAA+AGG | 0.583633 | 6.1:+16615392 | MS.gene85726:CDS |
| CGCATGATCCTGCATTGCAG+CGG | 0.584928 | 6.1:+16614848 | MS.gene85726:CDS |
| AAACAAACACTCGGGGAGTG+AGG | 0.585505 | 6.1:+16614345 | MS.gene85726:CDS |
| GTCCACTTCACACGCTGCCA+AGG | 0.587195 | 6.1:-16614433 | None:intergenic |
| TTAGCAAACCTAGTTAAAGC+TGG | 0.588173 | 6.1:-16615427 | None:intergenic |
| TGACCATCACCACCTTCCTC+CGG | 0.589498 | 6.1:-16614376 | None:intergenic |
| TTCGTATCCTCATGGACAGA+TGG | 0.597348 | 6.1:+16615134 | MS.gene85726:CDS |
| TCCAAGGTTATGGCTGAAAG+GGG | 0.597943 | 6.1:+16614583 | MS.gene85726:CDS |
| GGAGATAGAAACAAACACTC+GGG | 0.601048 | 6.1:+16614337 | MS.gene85726:CDS |
| CTTAACGATATGCTTGGAAG+GGG | 0.602105 | 6.1:+16614670 | MS.gene85726:CDS |
| GAGTGAGGAAGATGAGCCGG+AGG | 0.604600 | 6.1:+16614360 | MS.gene85726:CDS |
| GGACAGATGGTACAACCAGA+TGG | 0.605592 | 6.1:+16615147 | MS.gene85726:CDS |
| CATCATCAAGAAGGAAGTAG+AGG | 0.606503 | 6.1:+16614397 | MS.gene85726:CDS |
| AGTTCTGAAGGTGGTAGTGG+TGG | 0.609983 | 6.1:+16614511 | MS.gene85726:CDS |
| TCGATGCGAGTTCATCGTCA+AGG | 0.616549 | 6.1:+16614169 | MS.gene85726:CDS |
| AATTTATCCTCACATTGCTG+AGG | 0.616974 | 6.1:-16614619 | None:intergenic |
| GAAGATGAGCCGGAGGAAGG+TGG | 0.617091 | 6.1:+16614367 | MS.gene85726:CDS |
| GGTGATGGTCATCATCAAGA+AGG | 0.617927 | 6.1:+16614388 | MS.gene85726:CDS |
| ATTTCGGAGAGCTATTTGCA+AGG | 0.617973 | 6.1:-16614872 | None:intergenic |
| GAAGAGAACTACGCTTCCCA+TGG | 0.620396 | 6.1:+16614991 | MS.gene85726:CDS |
| TGTGAAGTGGACTGATAAGA+TGG | 0.627029 | 6.1:+16614444 | MS.gene85726:CDS |
| ATGATAACGATGATGTGAGA+AGG | 0.627318 | 6.1:+16614905 | MS.gene85726:CDS |
| ATCATCAAGAAGGAAGTAGA+GGG | 0.627850 | 6.1:+16614398 | MS.gene85726:CDS |
| GGGAGATAGAAACAAACACT+CGG | 0.632973 | 6.1:+16614336 | MS.gene85726:CDS |
| CGATGCGAGTTCATCGTCAA+GGG | 0.633456 | 6.1:+16614170 | MS.gene85726:CDS |
| TTAATCGAAGAAAGCACCGC+CGG | 0.638253 | 6.1:-16614241 | None:intergenic |
| ATGGAAGGGAATTTGTCTCA+AGG | 0.640788 | 6.1:+16614085 | MS.gene85726:CDS |
| TGTTGTTGAGAAGAAATGGA+AGG | 0.642321 | 6.1:+16614070 | None:intergenic |
| GCTTCCCATGGTGATAGTCG+AGG | 0.642635 | 6.1:+16615003 | MS.gene85726:CDS |
| GTTGTTGAGAAGAAATGGAA+GGG | 0.643736 | 6.1:+16614071 | None:intergenic |
| GATAGTCGAGGAATCTTTGG+GGG | 0.644442 | 6.1:+16615015 | MS.gene85726:CDS |
| GCAATGGGTTGAATCTCGCT+CGG | 0.646017 | 6.1:+16615215 | MS.gene85726:CDS |
| TCGATGAACGAGTTTAGTCA+GGG | 0.648981 | 6.1:+16614316 | MS.gene85726:CDS |
| TCATCCGTGTCAAGGGCCGG+CGG | 0.649182 | 6.1:+16614225 | MS.gene85726:CDS |
| GGCGGTGTCTTATATTGGCG+AGG | 0.657443 | 6.1:+16614483 | MS.gene85726:CDS |
| GATTCCTCGACTATCACCAT+GGG | 0.658101 | 6.1:-16615007 | None:intergenic |
| CTATCATGATGATCATGACG+AGG | 0.661777 | 6.1:+16614930 | MS.gene85726:CDS |
| AGATTCCTCGACTATCACCA+TGG | 0.673633 | 6.1:-16615008 | None:intergenic |
| GGTTGTACCATCTGTCCATG+AGG | 0.678583 | 6.1:-16615141 | None:intergenic |
| TGATAGTCGAGGAATCTTTG+GGG | 0.690169 | 6.1:+16615014 | MS.gene85726:CDS |
| AGAAGACTAAGACAAGGCCA+AGG | 0.695160 | 6.1:+16615054 | MS.gene85726:CDS |
| CTGAAGCGAAAGGAGATCGG+CGG | 0.699525 | 6.1:+16615402 | MS.gene85726:CDS |
| TGTTTCGCCTCAGCAATGTG+AGG | 0.699798 | 6.1:+16614612 | MS.gene85726:CDS |
| GAAGGGAATTTGTCTCAAGG+AGG | 0.706788 | 6.1:+16614088 | MS.gene85726:CDS |
| GAGATAGAAACAAACACTCG+GGG | 0.708162 | 6.1:+16614338 | MS.gene85726:CDS |
| GAGGACGGGAGTTCTGAAGG+TGG | 0.721621 | 6.1:+16614502 | MS.gene85726:CDS |
| AGTGGACTGATAAGATGGTG+AGG | 0.726752 | 6.1:+16614449 | MS.gene85726:CDS |
| ACTACGAGAAGACTAAGACA+AGG | 0.742422 | 6.1:+16615048 | MS.gene85726:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTAATAAAAGGTATAAA+AGG | + | chr6.1:16614647-16614666 | MS.gene85726:CDS | 15.0% |
| !! | AATGCAATCTATTACAATTA+TGG | - | chr6.1:16614825-16614844 | None:intergenic | 20.0% |
| !! | AATTCAACGATCTTAATAAA+AGG | + | chr6.1:16614638-16614657 | MS.gene85726:CDS | 20.0% |
| !! | AGAGAAACAAAAATTCAAAT+GGG | + | chr6.1:16615287-16615306 | MS.gene85726:CDS | 20.0% |
| !! | TAGAGAAACAAAAATTCAAA+TGG | + | chr6.1:16615286-16615305 | MS.gene85726:CDS | 20.0% |
| ! | TATCATGATCATCTCTATTT+CGG | - | chr6.1:16614891-16614910 | None:intergenic | 25.0% |
| ! | TGGAAATTCTTTAAATTGTC+AGG | + | chr6.1:16615098-16615117 | MS.gene85726:CDS | 25.0% |
| AACAAAAATTCAAATGGGAG+AGG | + | chr6.1:16615292-16615311 | MS.gene85726:CDS | 30.0% | |
| GAAATCTATTTCCAAGGTTA+TGG | + | chr6.1:16614573-16614592 | MS.gene85726:CDS | 30.0% | |
| GTATCTTAACGAGAAAGAAA+AGG | + | chr6.1:16614741-16614760 | MS.gene85726:CDS | 30.0% | |
| TAAGTGGAAATCTATTTCCA+AGG | + | chr6.1:16614567-16614586 | MS.gene85726:CDS | 30.0% | |
| ! | AAGGTTTTCCTCTTACAATA+GGG | + | chr6.1:16614266-16614285 | MS.gene85726:CDS | 30.0% |
| AAAAGGCTTAACGATATGCT+TGG | + | chr6.1:16614664-16614683 | MS.gene85726:CDS | 35.0% | |
| AATTTATCCTCACATTGCTG+AGG | - | chr6.1:16614622-16614641 | None:intergenic | 35.0% | |
| ACAGATTCAGATTGAGATGA+TGG | + | chr6.1:16615260-16615279 | MS.gene85726:CDS | 35.0% | |
| AGATGGAAATCAAGCTTTAC+CGG | + | chr6.1:16615164-16615183 | MS.gene85726:CDS | 35.0% | |
| ATCATCAAGAAGGAAGTAGA+GGG | + | chr6.1:16614398-16614417 | MS.gene85726:CDS | 35.0% | |
| ATTTGCAGTGTTGCAGAAAA+AGG | + | chr6.1:16614543-16614562 | MS.gene85726:CDS | 35.0% | |
| CAAAAGTAGTAGCATCTTCA+TGG | - | chr6.1:16615080-16615099 | None:intergenic | 35.0% | |
| CATAAAAGTTCGTATCCTCA+TGG | + | chr6.1:16615126-16615145 | MS.gene85726:CDS | 35.0% | |
| GGATGATCATGATATGGAAA+CGG | + | chr6.1:16614951-16614970 | MS.gene85726:CDS | 35.0% | |
| GTGCTTTCTTCGATTAATGA+AGG | + | chr6.1:16614247-16614266 | MS.gene85726:CDS | 35.0% | |
| TCAATTACATCCAACAAAGC+CGG | - | chr6.1:16614721-16614740 | None:intergenic | 35.0% | |
| TTTGCAGTGTTGCAGAAAAA+GGG | + | chr6.1:16614544-16614563 | MS.gene85726:CDS | 35.0% | |
| ! | ATGATAACGATGATGTGAGA+AGG | + | chr6.1:16614905-16614924 | MS.gene85726:CDS | 35.0% |
| ! | CATGAAGATGCTACTACTTT+TGG | + | chr6.1:16615078-16615097 | MS.gene85726:CDS | 35.0% |
| ! | CATGAGGATACGAACTTTTA+TGG | - | chr6.1:16615128-16615147 | None:intergenic | 35.0% |
| ! | CTGTCAAGTTGTTGAAAATC+CGG | + | chr6.1:16614699-16614718 | MS.gene85726:CDS | 35.0% |
| ! | GAAGGTTTTCCTCTTACAAT+AGG | + | chr6.1:16614265-16614284 | MS.gene85726:CDS | 35.0% |
| !!! | TTTTGGTGGTTTTGATTTGC+CGG | + | chr6.1:16614144-16614163 | MS.gene85726:CDS | 35.0% |
| AAGGAAGTAGAGGGAAAAAG+GGG | + | chr6.1:16614407-16614426 | MS.gene85726:CDS | 40.0% | |
| ACTACGAGAAGACTAAGACA+AGG | + | chr6.1:16615048-16615067 | MS.gene85726:CDS | 40.0% | |
| AGAAGGAAGTAGAGGGAAAA+AGG | + | chr6.1:16614405-16614424 | MS.gene85726:CDS | 40.0% | |
| AGCTTGGTTACAGAAGCAAT+GGG | + | chr6.1:16615200-16615219 | MS.gene85726:CDS | 40.0% | |
| ATAACGGCGGTGTCTTATAT+TGG | + | chr6.1:16614478-16614497 | MS.gene85726:CDS | 40.0% | |
| ATCAAACTCATCCGTGTCAA+GGG | + | chr6.1:16614218-16614237 | MS.gene85726:CDS | 40.0% | |
| ATGGAAGGGAATTTGTCTCA+AGG | + | chr6.1:16614085-16614104 | MS.gene85726:CDS | 40.0% | |
| ATTTCGGAGAGCTATTTGCA+AGG | - | chr6.1:16614875-16614894 | None:intergenic | 40.0% | |
| CAAGAAAAAAGACCGAGAGT+TGG | + | chr6.1:16615320-16615339 | MS.gene85726:CDS | 40.0% | |
| CATCATCAAGAAGGAAGTAG+AGG | + | chr6.1:16614397-16614416 | MS.gene85726:CDS | 40.0% | |
| CTATCATGATGATCATGACG+AGG | + | chr6.1:16614930-16614949 | MS.gene85726:CDS | 40.0% | |
| CTCGTCATGATCATCATGAT+AGG | - | chr6.1:16614932-16614951 | None:intergenic | 40.0% | |
| CTTAACGATATGCTTGGAAG+GGG | + | chr6.1:16614670-16614689 | MS.gene85726:CDS | 40.0% | |
| GAAGGAAGTAGAGGGAAAAA+GGG | + | chr6.1:16614406-16614425 | MS.gene85726:CDS | 40.0% | |
| GAGATAGAAACAAACACTCG+GGG | + | chr6.1:16614338-16614357 | MS.gene85726:CDS | 40.0% | |
| GCTTAACGATATGCTTGGAA+GGG | + | chr6.1:16614669-16614688 | MS.gene85726:CDS | 40.0% | |
| GGAGATAGAAACAAACACTC+GGG | + | chr6.1:16614337-16614356 | MS.gene85726:CDS | 40.0% | |
| GGGAGATAGAAACAAACACT+CGG | + | chr6.1:16614336-16614355 | MS.gene85726:CDS | 40.0% | |
| GGTAAAGCTTGATTTCCATC+TGG | - | chr6.1:16615165-16615184 | None:intergenic | 40.0% | |
| GTGATAGTCGAGGAATCTTT+GGG | + | chr6.1:16615013-16615032 | MS.gene85726:CDS | 40.0% | |
| TCGATGAACGAGTTTAGTCA+GGG | + | chr6.1:16614316-16614335 | MS.gene85726:CDS | 40.0% | |
| TGACGAGGATGATCATGATA+TGG | + | chr6.1:16614945-16614964 | MS.gene85726:CDS | 40.0% | |
| TGATAGTCGAGGAATCTTTG+GGG | + | chr6.1:16615014-16615033 | MS.gene85726:CDS | 40.0% | |
| TGTTGCAGAAAAAGGGTAAG+TGG | + | chr6.1:16614551-16614570 | MS.gene85726:CDS | 40.0% | |
| TTCAACGTCCCTATTGTAAG+AGG | - | chr6.1:16614277-16614296 | None:intergenic | 40.0% | |
| TTCCAAGGTTATGGCTGAAA+GGG | + | chr6.1:16614582-16614601 | MS.gene85726:CDS | 40.0% | |
| TTTCCAAGGTTATGGCTGAA+AGG | + | chr6.1:16614581-16614600 | MS.gene85726:CDS | 40.0% | |
| ! | CGGATTTTCAACAACTTGAC+AGG | - | chr6.1:16614701-16614720 | None:intergenic | 40.0% |
| ! | GATGGTGAGGCTTTTGATAA+CGG | + | chr6.1:16614462-16614481 | MS.gene85726:CDS | 40.0% |
| ! | TCTCAAGGAGGTATAGTTCA+AGG | + | chr6.1:16614100-16614119 | MS.gene85726:CDS | 40.0% |
| ! | TGTTGAAAATCCGGCTTTGT+TGG | + | chr6.1:16614708-16614727 | MS.gene85726:CDS | 40.0% |
| !! | CAAGCTGCTTTCATGTTTTC+CGG | - | chr6.1:16615186-16615205 | None:intergenic | 40.0% |
| !! | TGATGTTGGTTCATCGTATG+CGG | - | chr6.1:16614202-16614221 | None:intergenic | 40.0% |
| !! | TGCTTTAGAACTGAAGCGAA+AGG | + | chr6.1:16615392-16615411 | MS.gene85726:CDS | 40.0% |
| !! | TGTGAAGTGGACTGATAAGA+TGG | + | chr6.1:16614444-16614463 | MS.gene85726:CDS | 40.0% |
| !!! | TTTGGTGGTTTTGATTTGCC+GGG | + | chr6.1:16614145-16614164 | MS.gene85726:CDS | 40.0% |
| AGAAGACTAAGACAAGGCCA+AGG | + | chr6.1:16615054-16615073 | MS.gene85726:CDS | 45.0% | |
| AGAGTTGGAGAAGTTCAAGC+TGG | + | chr6.1:16615335-16615354 | MS.gene85726:CDS | 45.0% | |
| AGATTCCTCGACTATCACCA+TGG | - | chr6.1:16615011-16615030 | None:intergenic | 45.0% | |
| CAGCTTGGTTACAGAAGCAA+TGG | + | chr6.1:16615199-16615218 | MS.gene85726:CDS | 45.0% | |
| CATCAAACTCATCCGTGTCA+AGG | + | chr6.1:16614217-16614236 | MS.gene85726:CDS | 45.0% | |
| CGGAAAACATGAAAGCAGCT+TGG | + | chr6.1:16615184-16615203 | MS.gene85726:CDS | 45.0% | |
| GAACTGAAGCGAAAGGAGAT+CGG | + | chr6.1:16615399-16615418 | MS.gene85726:CDS | 45.0% | |
| GAAGGGAATTTGTCTCAAGG+AGG | + | chr6.1:16614088-16614107 | MS.gene85726:CDS | 45.0% | |
| GACACGGATGAGTTTGATGT+TGG | - | chr6.1:16614216-16614235 | None:intergenic | 45.0% | |
| GATAGTCGAGGAATCTTTGG+GGG | + | chr6.1:16615015-16615034 | MS.gene85726:CDS | 45.0% | |
| GATTCCTCGACTATCACCAT+GGG | - | chr6.1:16615010-16615029 | None:intergenic | 45.0% | |
| GCTTGAACTTCTCCAACTCT+CGG | - | chr6.1:16615335-16615354 | None:intergenic | 45.0% | |
| GGCTTAACGATATGCTTGGA+AGG | + | chr6.1:16614668-16614687 | MS.gene85726:CDS | 45.0% | |
| GGTGATAGTCGAGGAATCTT+TGG | + | chr6.1:16615012-16615031 | MS.gene85726:CDS | 45.0% | |
| GTCGATGAACGAGTTTAGTC+AGG | + | chr6.1:16614315-16614334 | MS.gene85726:CDS | 45.0% | |
| GTGTCTTATATTGGCGAGGA+CGG | + | chr6.1:16614487-16614506 | MS.gene85726:CDS | 45.0% | |
| TAGTAGCATCTTCATGGCCT+TGG | - | chr6.1:16615074-16615093 | None:intergenic | 45.0% | |
| TCCAAGGTTATGGCTGAAAG+GGG | + | chr6.1:16614583-16614602 | MS.gene85726:CDS | 45.0% | |
| TGTCTTATATTGGCGAGGAC+GGG | + | chr6.1:16614488-16614507 | MS.gene85726:CDS | 45.0% | |
| TTCGTATCCTCATGGACAGA+TGG | + | chr6.1:16615134-16615153 | MS.gene85726:CDS | 45.0% | |
| ! | GGAGGTATAGTTCAAGGTGA+TGG | + | chr6.1:16614106-16614125 | MS.gene85726:CDS | 45.0% |
| ! | GGTATAGTTCAAGGTGATGG+TGG | + | chr6.1:16614109-16614128 | MS.gene85726:CDS | 45.0% |
| ! | GGTGATGGTCATCATCAAGA+AGG | + | chr6.1:16614388-16614407 | MS.gene85726:CDS | 45.0% |
| !! | AGTGGACTGATAAGATGGTG+AGG | + | chr6.1:16614449-16614468 | MS.gene85726:CDS | 45.0% |
| !! | ATAGTTCAAGGTGATGGTGG+TGG | + | chr6.1:16614112-16614131 | MS.gene85726:CDS | 45.0% |
| !! | TTAATCGAAGAAAGCACCGC+CGG | - | chr6.1:16614244-16614263 | None:intergenic | 45.0% |
| AAACAAACACTCGGGGAGTG+AGG | + | chr6.1:16614345-16614364 | MS.gene85726:CDS | 50.0% | |
| ACCCCTTTCAGCCATAACCT+TGG | - | chr6.1:16614587-16614606 | None:intergenic | 50.0% | |
| CGATGCGAGTTCATCGTCAA+GGG | + | chr6.1:16614170-16614189 | MS.gene85726:CDS | 50.0% | |
| GAAGAGAACTACGCTTCCCA+TGG | + | chr6.1:16614991-16615010 | MS.gene85726:CDS | 50.0% | |
| GGACAGATGGTACAACCAGA+TGG | + | chr6.1:16615147-16615166 | MS.gene85726:CDS | 50.0% | |
| GGTTGTACCATCTGTCCATG+AGG | - | chr6.1:16615144-16615163 | None:intergenic | 50.0% | |
| TCGATGCGAGTTCATCGTCA+AGG | + | chr6.1:16614169-16614188 | MS.gene85726:CDS | 50.0% | |
| TGTTTCGCCTCAGCAATGTG+AGG | + | chr6.1:16614612-16614631 | MS.gene85726:CDS | 50.0% | |
| ! | AGGAATCTTTGGGGGATTGG+GGG | + | chr6.1:16615023-16615042 | MS.gene85726:CDS | 50.0% |
| ! | CGAGGAATCTTTGGGGGATT+GGG | + | chr6.1:16615021-16615040 | MS.gene85726:CDS | 50.0% |
| ! | GAGGAATCTTTGGGGGATTG+GGG | + | chr6.1:16615022-16615041 | MS.gene85726:CDS | 50.0% |
| ! | TCGAGGAATCTTTGGGGGAT+TGG | + | chr6.1:16615020-16615039 | MS.gene85726:CDS | 50.0% |
| !! | AGTTCTGAAGGTGGTAGTGG+TGG | + | chr6.1:16614511-16614530 | MS.gene85726:CDS | 50.0% |
| !! | GCAATGGGTTGAATCTCGCT+CGG | + | chr6.1:16615215-16615234 | MS.gene85726:CDS | 50.0% |
| !!! | GGTGAGGCTTTTGATAACGG+CGG | + | chr6.1:16614465-16614484 | MS.gene85726:CDS | 50.0% |
| AACTCATCCGTGTCAAGGGC+CGG | + | chr6.1:16614222-16614241 | MS.gene85726:CDS | 55.0% | |
| CGCATGATCCTGCATTGCAG+CGG | + | chr6.1:16614848-16614867 | MS.gene85726:CDS | 55.0% | |
| CGCTGCAATGCAGGATCATG+CGG | - | chr6.1:16614850-16614869 | None:intergenic | 55.0% | |
| CTGAAGCGAAAGGAGATCGG+CGG | + | chr6.1:16615402-16615421 | MS.gene85726:CDS | 55.0% | |
| GCTTCCCATGGTGATAGTCG+AGG | + | chr6.1:16615003-16615022 | MS.gene85726:CDS | 55.0% | |
| GGCGGTGTCTTATATTGGCG+AGG | + | chr6.1:16614483-16614502 | MS.gene85726:CDS | 55.0% | |
| TGACCATCACCACCTTCCTC+CGG | - | chr6.1:16614379-16614398 | None:intergenic | 55.0% | |
| ! | CGATGAACTCGCATCGATCC+CGG | - | chr6.1:16614166-16614185 | None:intergenic | 55.0% |
| ! | GGAATCTTTGGGGGATTGGG+GGG | + | chr6.1:16615024-16615043 | MS.gene85726:CDS | 55.0% |
| !! | CTGAAGGTGGTAGTGGTGGA+AGG | + | chr6.1:16614515-16614534 | MS.gene85726:CDS | 55.0% |
| !! | GGGAGTTCTGAAGGTGGTAG+TGG | + | chr6.1:16614508-16614527 | MS.gene85726:CDS | 55.0% |
| !! | GTTCAAGGTGATGGTGGTGG+TGG | + | chr6.1:16614115-16614134 | MS.gene85726:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGGTTCTTT+TGG | + | chr6.1:16614127-16614146 | MS.gene85726:CDS | 55.0% |
| !!! | GGTGGTGGTGGTTCTTTTGG+TGG | + | chr6.1:16614130-16614149 | MS.gene85726:CDS | 55.0% |
| GAAGATGAGCCGGAGGAAGG+TGG | + | chr6.1:16614367-16614386 | MS.gene85726:CDS | 60.0% | |
| GAGGAAGATGAGCCGGAGGA+AGG | + | chr6.1:16614364-16614383 | MS.gene85726:CDS | 60.0% | |
| GAGGGAAAAAGGGGTCGCCT+TGG | + | chr6.1:16614416-16614435 | MS.gene85726:CDS | 60.0% | |
| GAGTGAGGAAGATGAGCCGG+AGG | + | chr6.1:16614360-16614379 | MS.gene85726:CDS | 60.0% | |
| GGGGAGTGAGGAAGATGAGC+CGG | + | chr6.1:16614357-16614376 | MS.gene85726:CDS | 60.0% | |
| GTCCACTTCACACGCTGCCA+AGG | - | chr6.1:16614436-16614455 | None:intergenic | 60.0% | |
| TGCAAGGACCGCTGCAATGC+AGG | - | chr6.1:16614859-16614878 | None:intergenic | 60.0% | |
| !! | CAAGGTGATGGTGGTGGTGG+TGG | + | chr6.1:16614118-16614137 | MS.gene85726:CDS | 60.0% |
| !! | GAGGACGGGAGTTCTGAAGG+TGG | + | chr6.1:16614502-16614521 | MS.gene85726:CDS | 60.0% |
| CGCCTTGGCAGCGTGTGAAG+TGG | + | chr6.1:16614431-16614450 | MS.gene85726:CDS | 65.0% | |
| GAGCCGGAGGAAGGTGGTGA+TGG | + | chr6.1:16614373-16614392 | MS.gene85726:CDS | 65.0% | |
| TCATCCGTGTCAAGGGCCGG+CGG | + | chr6.1:16614225-16614244 | MS.gene85726:CDS | 65.0% | |
| ! | GGCGAGGACGGGAGTTCTGA+AGG | + | chr6.1:16614499-16614518 | MS.gene85726:CDS | 65.0% |
| AGCACCGCCGGCCCTTGACA+CGG | - | chr6.1:16614232-16614251 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 16614085 | 16615440 | 16614085 | ID=MS.gene85726 |
| chr6.1 | mRNA | 16614085 | 16615440 | 16614085 | ID=MS.gene85726.t1;Parent=MS.gene85726 |
| chr6.1 | exon | 16614085 | 16615440 | 16614085 | ID=MS.gene85726.t1.exon1;Parent=MS.gene85726.t1 |
| chr6.1 | CDS | 16614085 | 16615440 | 16614085 | ID=cds.MS.gene85726.t1;Parent=MS.gene85726.t1 |
| Gene Sequence |
| Protein sequence |
