AlfalfaGEDB | MS.gene89888

M. sativa cultivar XinJiangDaYe / MS.gene89888

KEGG: K18998
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89888.t1	RHN46413.1	97.2	142	4	0	1	142	107	248	8.10E-72	279.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89888.t1	Q5YDB5	66.7	144	45	2	1	142	107	249	1.1e-48	194.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89888.t1	G7KRB2	97.2	142	4	0	1	142	107	248	5.9e-72	279.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene89888	0.823328	1.47E-53	-1.69E-46
MS.gene04976	MS.gene89888	0.80027	1.56E-48	-1.69E-46
MS.gene05016	MS.gene89888	0.8165	5.36E-52	-1.69E-46
MS.gene050273	MS.gene89888	0.803027	4.24E-49	-1.69E-46
MS.gene050361	MS.gene89888	0.827649	1.39E-54	-1.69E-46
MS.gene050646	MS.gene89888	0.816217	6.20E-52	-1.69E-46
MS.gene050689	MS.gene89888	0.806272	8.91E-50	-1.69E-46
MS.gene050759	MS.gene89888	0.814351	1.61E-51	-1.69E-46
MS.gene050762	MS.gene89888	0.808673	2.76E-50	-1.69E-46
MS.gene050763	MS.gene89888	0.805055	1.61E-49	-1.69E-46
MS.gene050807	MS.gene89888	0.845386	4.19E-59	-1.69E-46
MS.gene050808	MS.gene89888	0.817215	3.70E-52	-1.69E-46
MS.gene050962	MS.gene89888	0.809105	2.23E-50	-1.69E-46
MS.gene050987	MS.gene89888	0.826325	2.89E-54	-1.69E-46
MS.gene051180	MS.gene89888	0.809655	1.70E-50	-1.69E-46
MS.gene051198	MS.gene89888	0.84648	2.12E-59	-1.69E-46
MS.gene051279	MS.gene89888	0.805517	1.28E-49	-1.69E-46
MS.gene051744	MS.gene89888	0.801629	8.23E-49	-1.69E-46
MS.gene051800	MS.gene89888	0.812622	3.86E-51	-1.69E-46
MS.gene052239	MS.gene89888	-0.82458	7.47E-54	-1.69E-46
MS.gene052242	MS.gene89888	-0.803402	3.55E-49	-1.69E-46
MS.gene052307	MS.gene89888	0.803736	3.02E-49	-1.69E-46
MS.gene052516	MS.gene89888	0.83005	3.65E-55	-1.69E-46
MS.gene052609	MS.gene89888	0.82711	1.87E-54	-1.69E-46
MS.gene052665	MS.gene89888	0.828131	1.07E-54	-1.69E-46
MS.gene053079	MS.gene89888	0.82236	2.47E-53	-1.69E-46
MS.gene05350	MS.gene89888	0.804781	1.83E-49	-1.69E-46
MS.gene053565	MS.gene89888	0.823137	1.63E-53	-1.69E-46
MS.gene05364	MS.gene89888	0.807874	4.08E-50	-1.69E-46
MS.gene054379	MS.gene89888	0.812845	3.45E-51	-1.69E-46
MS.gene054651	MS.gene89888	0.802418	5.66E-49	-1.69E-46
MS.gene055623	MS.gene89888	0.800797	1.22E-48	-1.69E-46
MS.gene055926	MS.gene89888	0.800789	1.22E-48	-1.69E-46
MS.gene05604	MS.gene89888	0.813077	3.07E-51	-1.69E-46
MS.gene056308	MS.gene89888	0.81626	6.06E-52	-1.69E-46
MS.gene056480	MS.gene89888	0.816354	5.77E-52	-1.69E-46
MS.gene056507	MS.gene89888	0.835115	2.03E-56	-1.69E-46
MS.gene057208	MS.gene89888	0.81577	7.80E-52	-1.69E-46
MS.gene057444	MS.gene89888	0.800213	1.60E-48	-1.69E-46
MS.gene057446	MS.gene89888	0.818187	2.23E-52	-1.69E-46
MS.gene057869	MS.gene89888	0.868284	7.32E-66	-1.69E-46
MS.gene057915	MS.gene89888	0.806246	9.03E-50	-1.69E-46
MS.gene058010	MS.gene89888	0.816199	6.25E-52	-1.69E-46
MS.gene058126	MS.gene89888	0.801047	1.08E-48	-1.69E-46
MS.gene058625	MS.gene89888	0.804734	1.87E-49	-1.69E-46
MS.gene058638	MS.gene89888	0.805501	1.29E-49	-1.69E-46
MS.gene058639	MS.gene89888	0.803991	2.68E-49	-1.69E-46
MS.gene059161	MS.gene89888	0.800781	1.23E-48	-1.69E-46
MS.gene059713	MS.gene89888	0.814011	1.91E-51	-1.69E-46
MS.gene059725	MS.gene89888	0.825457	4.64E-54	-1.69E-46
MS.gene05978	MS.gene89888	0.828038	1.12E-54	-1.69E-46
MS.gene059965	MS.gene89888	0.812473	4.16E-51	-1.69E-46
MS.gene060167	MS.gene89888	0.806436	8.23E-50	-1.69E-46
MS.gene060373	MS.gene89888	0.807081	6.02E-50	-1.69E-46
MS.gene06052	MS.gene89888	0.831228	1.88E-55	-1.69E-46
MS.gene060998	MS.gene89888	0.807642	4.58E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89888.t1	MTR_7g067540	97.183	142	4	0	1	142	107	248	4.00e-95	291
MS.gene89888.t1	MTR_4g089010	56.250	144	60	2	1	142	104	246	5.54e-50	171
MS.gene89888.t1	MTR_4g089000	76.000	50	12	0	9	58	27	76	7.07e-22	85.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89888.t1	AT5G01270	66.667	144	45	2	1	142	107	249	3.80e-61	201
MS.gene89888.t1	AT5G01270	66.667	144	45	2	1	142	107	249	3.88e-61	201
MS.gene89888.t1	AT4G21670	57.463	134	54	2	9	140	132	264	3.38e-46	160

Find 37 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGACTTTATGATGCGTGTT+TGG	0.317597	7.3:+37482793	MS.gene89888:CDS
AGACTTTATGATGCGTGTTT+GGG	0.320662	7.3:+37482794	MS.gene89888:CDS
CTGTTGTTAGGTTGCAAGAT+AGG	0.367044	7.3:+37483110	MS.gene89888:CDS
TGGTGAGTTGAAGCGGTATT+TGG	0.371306	7.3:+37482967	MS.gene89888:CDS
TGGGAATGGGAGGCAGTATC+AGG	0.372760	7.3:+37483039	MS.gene89888:CDS
AAGTTATGAGGCCTGTTGTT+AGG	0.379355	7.3:+37483098	MS.gene89888:CDS
GACTGATCCGTCGAGGGTGC+AGG	0.387810	7.3:+37482937	MS.gene89888:CDS
CACGCATCATAAAGTCTTGC+CGG	0.389093	7.3:-37482788	None:intergenic
TCGAGGGTGCAGGGGATGTC+TGG	0.406355	7.3:+37482947	MS.gene89888:CDS
GGAAATTTCTTCCGTTTGCT+CGG	0.416594	7.3:-37482743	None:intergenic
GGATTGATGCGTTGCGGAGT+TGG	0.424792	7.3:+37482903	MS.gene89888:CDS
CGTTGCGGAGTTGGCTTTCG+AGG	0.437532	7.3:+37482912	MS.gene89888:CDS
GAGAGTGATTCTGTTGTTGA+TGG	0.450057	7.3:+37483019	MS.gene89888:CDS
GTTTGGGGATGTTGAATTTG+AGG	0.453662	7.3:+37482810	MS.gene89888:CDS
CAACACGATGAAGTCGTTTG+AGG	0.456176	7.3:+37482877	MS.gene89888:CDS
TAGTGGCGATGCCGAGCAAA+CGG	0.458942	7.3:+37482732	None:intergenic
CGATGAAGTCGTTTGAGGAT+AGG	0.476388	7.3:+37482882	MS.gene89888:CDS
CTATCTTGCAACCTAACAAC+AGG	0.488040	7.3:-37483109	None:intergenic
GATTCTGTTGTTGATGGGAA+TGG	0.501214	7.3:+37483025	MS.gene89888:CDS
GAGGCAGTATCAGGTTCAAA+TGG	0.519359	7.3:+37483048	MS.gene89888:CDS
ATTCTGTTGTTGATGGGAAT+GGG	0.521221	7.3:+37483026	MS.gene89888:CDS
ACTGATCCGTCGAGGGTGCA+GGG	0.522133	7.3:+37482938	MS.gene89888:CDS
TGAGTTGAAGCGGTATTTGG+AGG	0.540094	7.3:+37482970	MS.gene89888:CDS
GAAACTAGAATACAAACCTC+AGG	0.542477	7.3:-37483157	None:intergenic
CTCAAACGACTTCATCGTGT+TGG	0.563017	7.3:-37482876	None:intergenic
GTTGCGGAGTTGGCTTTCGA+GGG	0.569283	7.3:+37482913	MS.gene89888:CDS
GACATCCCCTGCACCCTCGA+CGG	0.570217	7.3:-37482944	None:intergenic
GACTTTATGATGCGTGTTTG+GGG	0.583488	7.3:+37482795	MS.gene89888:CDS
AGGATAGGATTGATGCGTTG+CGG	0.583888	7.3:+37482897	MS.gene89888:CDS
AGAGTGATTCTGTTGTTGAT+GGG	0.591630	7.3:+37483020	MS.gene89888:CDS
CTGATCCGTCGAGGGTGCAG+GGG	0.607018	7.3:+37482939	MS.gene89888:CDS
GGATGTCTGGTGAGTTGAAG+CGG	0.609489	7.3:+37482960	MS.gene89888:CDS
CGAGGGAGACTGATCCGTCG+AGG	0.625690	7.3:+37482930	MS.gene89888:CDS
GAGGGAGACTGATCCGTCGA+GGG	0.636719	7.3:+37482931	MS.gene89888:CDS
TCTTACTCGAATCAATCCTG+AGG	0.649953	7.3:+37483141	MS.gene89888:CDS
GCAGTATCAGGTTCAAATGG+AGG	0.652407	7.3:+37483051	MS.gene89888:CDS
CTGTTGTTGATGGGAATGGG+AGG	0.661826	7.3:+37483029	MS.gene89888:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGTCTGAACAAAAAGTTATG+AGG	+	chr7.3:37483086-37483105	MS.gene89888:CDS	30.0%
!	ACTTTTTGTTCAGACAAAGA+CGG	-	chr7.3:37483082-37483101	None:intergenic	30.0%
	AAGACTTTATGATGCGTGTT+TGG	+	chr7.3:37482793-37482812	MS.gene89888:CDS	35.0%
	ACTGCATAACACCAAAAACA+CGG	-	chr7.3:37482767-37482786	None:intergenic	35.0%
	AGACTTTATGATGCGTGTTT+GGG	+	chr7.3:37482794-37482813	MS.gene89888:CDS	35.0%
	AGAGTGATTCTGTTGTTGAT+GGG	+	chr7.3:37483020-37483039	MS.gene89888:CDS	35.0%
	GAAACTAGAATACAAACCTC+AGG	-	chr7.3:37483160-37483179	None:intergenic	35.0%
!	ATTCTGTTGTTGATGGGAAT+GGG	+	chr7.3:37483026-37483045	MS.gene89888:CDS	35.0%
!	GGAAGAAATTTCCGTGTTTT+TGG	+	chr7.3:37482753-37482772	MS.gene89888:CDS	35.0%
!!!	TTTTTGGTGTTATGCAGTTC+CGG	+	chr7.3:37482769-37482788	MS.gene89888:CDS	35.0%
	AAGTTATGAGGCCTGTTGTT+AGG	+	chr7.3:37483098-37483117	MS.gene89888:CDS	40.0%
	CTATCTTGCAACCTAACAAC+AGG	-	chr7.3:37483112-37483131	None:intergenic	40.0%
	GACTTTATGATGCGTGTTTG+GGG	+	chr7.3:37482795-37482814	MS.gene89888:CDS	40.0%
	GAGAGTGATTCTGTTGTTGA+TGG	+	chr7.3:37483019-37483038	MS.gene89888:CDS	40.0%
	GGAAATTTCTTCCGTTTGCT+CGG	-	chr7.3:37482746-37482765	None:intergenic	40.0%
	GTTTGGGGATGTTGAATTTG+AGG	+	chr7.3:37482810-37482829	MS.gene89888:CDS	40.0%
	TCTTACTCGAATCAATCCTG+AGG	+	chr7.3:37483141-37483160	MS.gene89888:CDS	40.0%
!	CTGTTGTTAGGTTGCAAGAT+AGG	+	chr7.3:37483110-37483129	MS.gene89888:CDS	40.0%
!	GATTCTGTTGTTGATGGGAA+TGG	+	chr7.3:37483025-37483044	MS.gene89888:CDS	40.0%
	AGGATAGGATTGATGCGTTG+CGG	+	chr7.3:37482897-37482916	MS.gene89888:CDS	45.0%
	CAACACGATGAAGTCGTTTG+AGG	+	chr7.3:37482877-37482896	MS.gene89888:CDS	45.0%
	CACGCATCATAAAGTCTTGC+CGG	-	chr7.3:37482791-37482810	None:intergenic	45.0%
	CGATGAAGTCGTTTGAGGAT+AGG	+	chr7.3:37482882-37482901	MS.gene89888:CDS	45.0%
	CTCAAACGACTTCATCGTGT+TGG	-	chr7.3:37482879-37482898	None:intergenic	45.0%
	GAGGCAGTATCAGGTTCAAA+TGG	+	chr7.3:37483048-37483067	MS.gene89888:CDS	45.0%
	GCAGTATCAGGTTCAAATGG+AGG	+	chr7.3:37483051-37483070	MS.gene89888:CDS	45.0%
!	TGAGTTGAAGCGGTATTTGG+AGG	+	chr7.3:37482970-37482989	MS.gene89888:CDS	45.0%
!	TGGTGAGTTGAAGCGGTATT+TGG	+	chr7.3:37482967-37482986	MS.gene89888:CDS	45.0%
!	CTGTTGTTGATGGGAATGGG+AGG	+	chr7.3:37483029-37483048	MS.gene89888:CDS	50.0%
!	GGATGTCTGGTGAGTTGAAG+CGG	+	chr7.3:37482960-37482979	MS.gene89888:CDS	50.0%
	GGATTGATGCGTTGCGGAGT+TGG	+	chr7.3:37482903-37482922	MS.gene89888:CDS	55.0%
	TGGGAATGGGAGGCAGTATC+AGG	+	chr7.3:37483039-37483058	MS.gene89888:CDS	55.0%
!!	GTTGCGGAGTTGGCTTTCGA+GGG	+	chr7.3:37482913-37482932	MS.gene89888:CDS	55.0%
	GAGGGAGACTGATCCGTCGA+GGG	+	chr7.3:37482931-37482950	MS.gene89888:CDS	60.0%
!	ACTGATCCGTCGAGGGTGCA+GGG	+	chr7.3:37482938-37482957	MS.gene89888:CDS	60.0%
!!	CGTTGCGGAGTTGGCTTTCG+AGG	+	chr7.3:37482912-37482931	MS.gene89888:CDS	60.0%
	CGAGGGAGACTGATCCGTCG+AGG	+	chr7.3:37482930-37482949	MS.gene89888:CDS	65.0%
	GACATCCCCTGCACCCTCGA+CGG	-	chr7.3:37482947-37482966	None:intergenic	65.0%
!	CTGATCCGTCGAGGGTGCAG+GGG	+	chr7.3:37482939-37482958	MS.gene89888:CDS	65.0%
!	GACTGATCCGTCGAGGGTGC+AGG	+	chr7.3:37482937-37482956	MS.gene89888:CDS	65.0%
!	TCGAGGGTGCAGGGGATGTC+TGG	+	chr7.3:37482947-37482966	MS.gene89888:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	37482740	37483183	37482740	ID=MS.gene89888
chr7.3	mRNA	37482740	37483183	37482740	ID=MS.gene89888.t1;Parent=MS.gene89888
chr7.3	exon	37482740	37483183	37482740	ID=MS.gene89888.t1.exon1;Parent=MS.gene89888.t1
chr7.3	CDS	37482740	37483183	37482740	ID=cds.MS.gene89888.t1;Parent=MS.gene89888.t1

Gene Sequence

>MS.gene89888

ATGCCGAGCAAACGGAAGAAATTTCCGTGTTTTTGGTGTTATGCAGTTCCGGCAAGACTTTATGATGCGTGTTTGGGGATGTTGAATTTGAGGTGTTTATCAATTGTGTTTGATTTAGATGAGACTTTGATTGTTGCCAACACGATGAAGTCGTTTGAGGATAGGATTGATGCGTTGCGGAGTTGGCTTTCGAGGGAGACTGATCCGTCGAGGGTGCAGGGGATGTCTGGTGAGTTGAAGCGGTATTTGGAGGATAGATTGTTGTTGAAGCAGTTTGCTGAGAGTGATTCTGTTGTTGATGGGAATGGGAGGCAGTATCAGGTTCAAATGGAGGAAGTGCCGTCTTTGTCTGAACAAAAAGTTATGAGGCCTGTTGTTAGGTTGCAAGATAGGAATATTGTTCTTACTCGAATCAATCCTGAGGTTTGTATTCTAGTTTCTTAG

Protein sequence

>MS.gene89888.t1

MPSKRKKFPCFWCYAVPARLYDACLGMLNLRCLSIVFDLDETLIVANTMKSFEDRIDALRSWLSRETDPSRVQGMSGELKRYLEDRLLLKQFAESDSVVDGNGRQYQVQMEEVPSLSEQKVMRPVVRLQDRNIVLTRINPEVCILVS