AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90646


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90646.t1 MTR_2g085025 98.851 174 2 0 1 174 1 174 1.74e-123 345
MS.gene90646.t1 MTR_7g104020 67.742 155 48 1 20 174 14 166 1.10e-61 188
MS.gene90646.t1 MTR_7g104020 66.456 158 48 2 20 174 14 169 1.27e-59 183
MS.gene90646.t1 MTR_1g492930 56.329 158 68 1 17 173 1 158 3.93e-51 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90646.t1 AT2G27740 59.649 171 68 1 1 171 1 170 4.65e-68 205
MS.gene90646.t1 AT2G36410 67.647 136 44 0 38 173 57 192 2.01e-61 189
MS.gene90646.t1 AT3G52920 68.889 135 42 0 38 172 28 162 3.93e-61 187
MS.gene90646.t1 AT3G09980 70.139 144 43 0 31 174 35 178 9.03e-60 184
MS.gene90646.t1 AT2G36410 66.187 139 44 1 38 173 57 195 2.42e-59 183
MS.gene90646.t1 AT3G52920 67.391 138 42 1 38 172 28 165 6.10e-59 182
MS.gene90646.t1 AT5G03660 66.418 134 45 0 38 171 36 169 2.16e-49 157
MS.gene90646.t1 AT3G52900 51.974 152 73 0 23 174 13 164 1.47e-48 155
MS.gene90646.t1 AT2G36355 51.299 154 69 2 22 173 5 154 1.67e-44 144
MS.gene90646.t1 AT2G27740 53.659 123 56 1 1 123 1 122 1.12e-41 136
MS.gene90646.t1 AT5G03660 63.793 116 42 0 38 153 36 151 8.52e-40 132
MS.gene90646.t1 AT2G36410 66.279 86 29 0 38 123 57 142 3.19e-35 120
MS.gene90646.t1 AT5G03660 53.731 134 40 1 38 171 36 147 9.69e-32 112
MS.gene90646.t1 AT2G36355 62.651 83 31 0 91 173 1 83 1.15e-30 107
MS.gene90646.t1 AT2G36355 61.628 86 33 0 88 173 27 112 1.43e-30 107
MS.gene90646.t1 AT2G36355 44.144 111 55 3 22 130 5 110 3.07e-22 87.0

Find 29 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTAACCTCCACTAATT+TGG 0.202427 2.3:-12845386 None:intergenic
AGAAATTGAGAGAAGAAAAT+TGG 0.229998 2.3:+12844042 MS.gene90646:CDS
ATTGCAACATCCTTCCTCTT+TGG 0.283049 2.3:-12844696 None:intergenic
TTTCATCTCTTCTTGGACTC+TGG 0.297684 2.3:-12843967 None:intergenic
GGGTCAAACCTGCCAGAAAA+AGG 0.317850 2.3:+12844764 MS.gene90646:CDS
GTGCAACATGTGAAGAATTC+AGG 0.351410 2.3:+12843929 MS.gene90646:CDS
TCTAGCAATGTTTAGAGAAA+AGG 0.359449 2.3:+12844015 MS.gene90646:CDS
TTCTGGCAGGTTTGACCCAA+TGG 0.366095 2.3:-12844759 None:intergenic
AAAATTGGAGGTTAGAGATA+AGG 0.419428 2.3:+12844057 MS.gene90646:CDS
GAGTCCAAGAAGAGATGAAA+AGG 0.433573 2.3:+12843970 MS.gene90646:CDS
AACAAAGAGTTGAAGCCATT+GGG 0.443322 2.3:+12844744 MS.gene90646:CDS
AGAAATTGCATAGGAGATTG+AGG 0.462032 2.3:+12844662 MS.gene90646:intron
GAGGATAAGGATGAAGAAGC+TGG 0.468797 2.3:+12845536 MS.gene90646:CDS
CGAAAAGGAAACAAAGAGAT+TGG 0.472358 2.3:+12844102 MS.gene90646:CDS
TCAACTTGTTACCAAATTAG+TGG 0.474598 2.3:+12845375 MS.gene90646:CDS
GAAATTGCATAGGAGATTGA+GGG 0.490039 2.3:+12844663 MS.gene90646:intron
TAACAAAGAGTTGAAGCCAT+TGG 0.493465 2.3:+12844743 MS.gene90646:CDS
GATAAGGTTCATGCTCATCT+TGG 0.506717 2.3:+12844073 MS.gene90646:CDS
TCATCTTGGTCGAGTCGAAA+AGG 0.517837 2.3:+12844087 MS.gene90646:CDS
TGACTGAAAGTGAGAGGATA+AGG 0.518680 2.3:+12845523 MS.gene90646:CDS
AAGAAGAGATGAAAAGGAAG+AGG 0.527146 2.3:+12843976 MS.gene90646:CDS
AATTGAGAGAAGAAAATTGG+AGG 0.531288 2.3:+12844045 MS.gene90646:CDS
AGAATACAATGAAGCTCTTG+AGG 0.569694 2.3:+12845324 MS.gene90646:CDS
TTTCACAGATCCAAAGAGGA+AGG 0.618763 2.3:+12844686 MS.gene90646:CDS
AGGGTTTCACAGATCCAAAG+AGG 0.622777 2.3:+12844682 MS.gene90646:CDS
AGCTTGTGACTGAAAGTGAG+AGG 0.623994 2.3:+12845517 MS.gene90646:intron
ACTTGTTACCAAATTAGTGG+AGG 0.627774 2.3:+12845378 MS.gene90646:CDS
GATAAGGATGAAGAAGCTGG+AGG 0.694341 2.3:+12845539 MS.gene90646:CDS
AGTTAGAAAGAAGATAGACA+TGG 0.715757 2.3:+12844719 MS.gene90646:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTAAAAACAATGTA+CGG - chr2.3:12845219-12845238 None:intergenic 10.0%
!! ATATGTTTATTTAGTTGTTT+AGG + chr2.3:12845005-12845024 MS.gene90646:intron 15.0%
!! GAAATTAAAGCAAATAATTA+AGG - chr2.3:12844941-12844960 None:intergenic 15.0%
!! TTATAATATCTTTACAACAT+AGG + chr2.3:12844834-12844853 MS.gene90646:intron 15.0%
!!! AATTATTTGCTTTAATTTCT+TGG + chr2.3:12844942-12844961 MS.gene90646:intron 15.0%
!!! TTGTGAAACTATATATTTTT+GGG + chr2.3:12845289-12845308 MS.gene90646:intron 15.0%
!! AAAAAAGAAATATGTTAGCT+AGG - chr2.3:12845462-12845481 None:intergenic 20.0%
!! ATTAAAGCAAATAATTAAGG+TGG - chr2.3:12844938-12844957 None:intergenic 20.0%
!! CAAACAAAGATTGTTTATAA+TGG - chr2.3:12844642-12844661 None:intergenic 20.0%
!! TTTGATATTGTAGAAAATCA+CGG + chr2.3:12844340-12844359 MS.gene90646:intron 20.0%
!!! CTTGTGAAACTATATATTTT+TGG + chr2.3:12845288-12845307 MS.gene90646:intron 20.0%
!!! CTTTTGATGACATAATATAT+GGG - chr2.3:12844969-12844988 None:intergenic 20.0%
!!! TGTGAAACTATATATTTTTG+GGG + chr2.3:12845290-12845309 MS.gene90646:intron 20.0%
! AAGTTTCAAAATAATGGTCA+CGG + chr2.3:12844293-12844312 MS.gene90646:intron 25.0%
! AGAAATTGAGAGAAGAAAAT+TGG + chr2.3:12844042-12844061 MS.gene90646:CDS 25.0%
! ATATCAAAAGCAAAACACAT+TGG - chr2.3:12844885-12844904 None:intergenic 25.0%
! ATGATGTTTCATTGTTTGTT+TGG + chr2.3:12844181-12844200 MS.gene90646:intron 25.0%
! CAAATTCAGTGTTTGTTTAT+TGG - chr2.3:12844148-12844167 None:intergenic 25.0%
! CAATGAGAAAAACAAAGAAA+AGG + chr2.3:12845351-12845370 MS.gene90646:CDS 25.0%
! CCATATATTATGTCATCAAA+AGG + chr2.3:12844967-12844986 MS.gene90646:intron 25.0%
! TATATTATGTCATCAAAAGG+AGG + chr2.3:12844970-12844989 MS.gene90646:intron 25.0%
! TATCAAAAGCAAAACACATT+GGG - chr2.3:12844884-12844903 None:intergenic 25.0%
! TGTGCAAAGTTTCAAAATAA+TGG + chr2.3:12844287-12844306 MS.gene90646:intron 25.0%
!!! ATTGTTTGTTTGGTTTTTAC+TGG + chr2.3:12844191-12844210 MS.gene90646:intron 25.0%
!!! CCTTTTGATGACATAATATA+TGG - chr2.3:12844970-12844989 None:intergenic 25.0%
!!! TTCACTTGTTTGGTTTTTAT+TGG + chr2.3:12844244-12844263 MS.gene90646:intron 25.0%
AAAATTGGAGGTTAGAGATA+AGG + chr2.3:12844057-12844076 MS.gene90646:CDS 30.0%
AACAGTTAGTACTAAACAGT+TGG - chr2.3:12844799-12844818 None:intergenic 30.0%
AATTGAGAGAAGAAAATTGG+AGG + chr2.3:12844045-12844064 MS.gene90646:CDS 30.0%
AATTTGAATTCCGTCTGTTT+CGG - chr2.3:12845142-12845161 None:intergenic 30.0%
ACAATATAACGTTGAAATCG+TGG + chr2.3:12844413-12844432 MS.gene90646:intron 30.0%
AGTTAGAAAGAAGATAGACA+TGG + chr2.3:12844719-12844738 MS.gene90646:CDS 30.0%
TAAAGCTAATCCTTAAATCC+TGG - chr2.3:12844477-12844496 None:intergenic 30.0%
TCTAGCAATGTTTAGAGAAA+AGG + chr2.3:12844015-12844034 MS.gene90646:CDS 30.0%
TTAAAAACAATGTACGGAAG+AGG - chr2.3:12845213-12845232 None:intergenic 30.0%
! CTTTGCATTATGTGAACTTT+TGG + chr2.3:12845491-12845510 MS.gene90646:intron 30.0%
! CTTTGTTTGAGAAATTGCAT+AGG + chr2.3:12844653-12844672 MS.gene90646:intron 30.0%
! TACAACATAGGATTTGTTCA+AGG + chr2.3:12844846-12844865 MS.gene90646:intron 30.0%
! TGCTGATCATATGATCTTTT+TGG - chr2.3:12843893-12843912 None:intergenic 30.0%
! TGGAAAATCTAACCTTTTTC+TGG - chr2.3:12844779-12844798 None:intergenic 30.0%
!! TCAACTTGTTACCAAATTAG+TGG + chr2.3:12845375-12845394 MS.gene90646:CDS 30.0%
!! TTTTAAAAAACGGTCTGCAA+CGG - chr2.3:12844448-12844467 None:intergenic 30.0%
!!! CTATATATTTTTGGGGTTAC+AGG + chr2.3:12845297-12845316 MS.gene90646:intron 30.0%
!!! CTGGTCAAGGTTTTAAAAAA+CGG - chr2.3:12844458-12844477 None:intergenic 30.0%
AAAACCTTGACCAGGATTTA+AGG + chr2.3:12844464-12844483 MS.gene90646:intron 35.0%
AACAAAGAGTTGAAGCCATT+GGG + chr2.3:12844744-12844763 MS.gene90646:CDS 35.0%
AAGAAGAGATGAAAAGGAAG+AGG + chr2.3:12843976-12843995 MS.gene90646:CDS 35.0%
ACTTCTAACCTCCACTAATT+TGG - chr2.3:12845389-12845408 None:intergenic 35.0%
AGAAATTGCATAGGAGATTG+AGG + chr2.3:12844662-12844681 MS.gene90646:intron 35.0%
AGAATACAATGAAGCTCTTG+AGG + chr2.3:12845324-12845343 MS.gene90646:CDS 35.0%
AGACTAAAGTCTAAGCAATG+TGG - chr2.3:12844910-12844929 None:intergenic 35.0%
CAATATCAAAGATTACTGCG+TGG - chr2.3:12844330-12844349 None:intergenic 35.0%
CGAAAAGGAAACAAAGAGAT+TGG + chr2.3:12844102-12844121 MS.gene90646:CDS 35.0%
CGGAATTCAAATTTCTCAGT+TGG + chr2.3:12845149-12845168 MS.gene90646:intron 35.0%
GAAATTGCATAGGAGATTGA+GGG + chr2.3:12844663-12844682 MS.gene90646:intron 35.0%
TAACAAAGAGTTGAAGCCAT+TGG + chr2.3:12844743-12844762 MS.gene90646:CDS 35.0%
TAATCCTTAAATCCTGGTCA+AGG - chr2.3:12844471-12844490 None:intergenic 35.0%
TAGCGACCACAATTTGATAT+CGG + chr2.3:12844388-12844407 MS.gene90646:intron 35.0%
TGTGCAAAGTTTCACTTGTT+TGG + chr2.3:12844234-12844253 MS.gene90646:intron 35.0%
TTGTCACCGATATCAAATTG+TGG - chr2.3:12844397-12844416 None:intergenic 35.0%
! ACTTGTTACCAAATTAGTGG+AGG + chr2.3:12845378-12845397 MS.gene90646:CDS 35.0%
! TCTTCCTTTTCATCTCTTCT+TGG - chr2.3:12843977-12843996 None:intergenic 35.0%
!! AAATCTAACCTTTTTCTGGC+AGG - chr2.3:12844775-12844794 None:intergenic 35.0%
!! CTAACTAGCTTGTTTCATAC+AGG - chr2.3:12844534-12844553 None:intergenic 35.0%
!!! CGTTTTTTAAAACCTTGACC+AGG + chr2.3:12844456-12844475 MS.gene90646:intron 35.0%
ATTGCAACATCCTTCCTCTT+TGG - chr2.3:12844699-12844718 None:intergenic 40.0%
GACTTTGTAACCGAAACAGA+CGG + chr2.3:12845129-12845148 MS.gene90646:intron 40.0%
GAGTCCAAGAAGAGATGAAA+AGG + chr2.3:12843970-12843989 MS.gene90646:CDS 40.0%
GATAAGGTTCATGCTCATCT+TGG + chr2.3:12844073-12844092 MS.gene90646:CDS 40.0%
GTCAATCTCTTACCACCAAA+CGG - chr2.3:12845112-12845131 None:intergenic 40.0%
GTGCAACATGTGAAGAATTC+AGG + chr2.3:12843929-12843948 MS.gene90646:CDS 40.0%
TCAAATTGTGGTCGCTATTG+CGG - chr2.3:12844385-12844404 None:intergenic 40.0%
TGACTGAAAGTGAGAGGATA+AGG + chr2.3:12845523-12845542 MS.gene90646:CDS 40.0%
TTTCACAGATCCAAAGAGGA+AGG + chr2.3:12844686-12844705 MS.gene90646:CDS 40.0%
TTTCATCTCTTCTTGGACTC+TGG - chr2.3:12843970-12843989 None:intergenic 40.0%
AGCTTGTGACTGAAAGTGAG+AGG + chr2.3:12845517-12845536 MS.gene90646:intron 45.0%
AGGGTTTCACAGATCCAAAG+AGG + chr2.3:12844682-12844701 MS.gene90646:CDS 45.0%
ATCTCTTACCACCAAACGGA+AGG - chr2.3:12845108-12845127 None:intergenic 45.0%
GAGGATAAGGATGAAGAAGC+TGG + chr2.3:12845536-12845555 MS.gene90646:CDS 45.0%
GATAAGGATGAAGAAGCTGG+AGG + chr2.3:12845539-12845558 MS.gene90646:CDS 45.0%
TCATCTTGGTCGAGTCGAAA+AGG + chr2.3:12844087-12844106 MS.gene90646:CDS 45.0%
TGCTTCTTGTTCCTTCCGTT+TGG + chr2.3:12845094-12845113 MS.gene90646:intron 45.0%
TTCTTGTTCCTTCCGTTTGG+TGG + chr2.3:12845097-12845116 MS.gene90646:intron 45.0%
GGGTCAAACCTGCCAGAAAA+AGG + chr2.3:12844764-12844783 MS.gene90646:CDS 50.0%
TTCTGGCAGGTTTGACCCAA+TGG - chr2.3:12844762-12844781 None:intergenic 50.0%
! TAATGGTCACGGTCGCTTTG+CGG + chr2.3:12844304-12844323 MS.gene90646:intron 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12843881 12845593 12843881 ID=MS.gene90646
chr2.3 mRNA 12843881 12845593 12843881 ID=MS.gene90646.t1;Parent=MS.gene90646
chr2.3 exon 12843881 12844138 12843881 ID=MS.gene90646.t1.exon1;Parent=MS.gene90646.t1
chr2.3 CDS 12843881 12844138 12843881 ID=cds.MS.gene90646.t1;Parent=MS.gene90646.t1
chr2.3 exon 12844675 12844785 12844675 ID=MS.gene90646.t1.exon2;Parent=MS.gene90646.t1
chr2.3 CDS 12844675 12844785 12844675 ID=cds.MS.gene90646.t1;Parent=MS.gene90646.t1
chr2.3 exon 12845319 12845399 12845319 ID=MS.gene90646.t1.exon3;Parent=MS.gene90646.t1
chr2.3 CDS 12845319 12845399 12845319 ID=cds.MS.gene90646.t1;Parent=MS.gene90646.t1
chr2.3 exon 12845519 12845593 12845519 ID=MS.gene90646.t1.exon4;Parent=MS.gene90646.t1
chr2.3 CDS 12845519 12845593 12845519 ID=cds.MS.gene90646.t1;Parent=MS.gene90646.t1
Gene Sequence

>MS.gene90646

ATGGCAACACCAAAAAGATCATATGATCAGCAACAACAACTACAGATTGTGCAACATGTGAAGAATTCAGGAATGATTAACAACATCCAGAGTCCAAGAAGAGATGAAAAGGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGAAAAGGAAGAAGAAATTGAGAGAAGAAAATTGGAGGTTAGAGATAAGGTTCATGCTCATCTTGGTCGAGTCGAAAAGGAAACAAAGAGATTGGCTGAGATAAGAGAAGTAAGTCCAATAAACAAACACTGAATTTGAGCTAGATTGTTTATGATGTTTCATTGTTTGTTTGGTTTTTACTGGTTTATGCATTTATAAATCATTGTGCAAAGTTTCACTTGTTTGGTTTTTATTGGTTTATGTATTTATAAATCTTTGTGCAAAGTTTCAAAATAATGGTCACGGTCGCTTTGCGGCCACGCAGTAATCTTTGATATTGTAGAAAATCACGGATAAATACAACTTGTGTTGCCGCAATAGCGACCACAATTTGATATCGGTGACAATATAACGTTGAAATCGTGGCTATATTTGCCGTTGCAGACCGTTTTTTAAAACCTTGACCAGGATTTAAGGATTAGCTTTACATTTGATCTAGAGTATTCACATGTTCATATATACCTGTATGAAACAAGCTAGTTAGAAACAATTTAAAAATAAAATAAAATCAAATGTGATATTTTATTACAAAATTATTCACATTTTGTTATATTCAAGATTTTTTTCATCCATTATAAACAATCTTTGTTTGAGAAATTGCATAGGAGATTGAGGGTTTCACAGATCCAAAGAGGAAGGATGTTGCAATAGTTAGAAAGAAGATAGACATGGTTAACAAAGAGTTGAAGCCATTGGGTCAAACCTGCCAGAAAAAGGTTAGATTTTCCAACTGTTTAGTACTAACTGTTGTTTGCTTTATTCTTTTATAATATCTTTACAACATAGGATTTGTTCAAGGAGACAAAGTGATCCCAATGTGTTTTGCTTTTGATATAACCACATTGCTTAGACTTTAGTCTATTGACCACCTTAATTATTTGCTTTAATTTCTTGGTCCCATATATTATGTCATCAAAAGGAGGCTATATAATACTATATGTTTATTTAGTTGTTTAGGTGATTATTCAAAATAATTGTGTTTGAATTTGCAAGTAGAAAATTGCTATTTGAAATTCGTCATACATGCTTCTTGTTCCTTCCGTTTGGTGGTAAGAGATTGACTTTGTAACCGAAACAGACGGAATTCAAATTTCTCAGTTGGAAAATGTCATTTTTGCAACTTTAATTTATGATAATTTTCCTCTTCCGTACATTGTTTTTAAAAAATAAATTAAAACAAGAATGAAAGAATGTTAACTATAAAAGTTTCTGAATCTTCTTGTGAAACTATATATTTTTGGGGTTACAGGAGAGAGAATACAATGAAGCTCTTGAGGCTTTCAATGAGAAAAACAAAGAAAAGGCTCAACTTGTTACCAAATTAGTGGAGGTTAGAAGTTATTCATTGATTTACATGTTAGTACACTAACAATATCATATATTTTTGTTCCTAGCTAACATATTTCTTTTTTATTCTCTCTCTTTGCATTATGTGAACTTTTGGATTAGCTTGTGACTGAAAGTGAGAGGATAAGGATGAAGAAGCTGGAGGATCTAAGCAAAAATATAGATACACTTCACTAA

Protein sequence

>MS.gene90646.t1

MATPKRSYDQQQQLQIVQHVKNSGMINNIQSPRRDEKEEEMSRSVLAMFREKEEEIERRKLEVRDKVHAHLGRVEKETKRLAEIREEIEGFTDPKRKDVAIVRKKIDMVNKELKPLGQTCQKKEREYNEALEAFNEKNKEKAQLVTKLVELVTESERIRMKKLEDLSKNIDTLH