AlfalfaGEDB | MS.gene91508

M. sativa cultivar XinJiangDaYe / MS.gene91508

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91508.t1	XP_013464881.1	87.6	97	7	1	1	97	1	92	1.30E-35	159.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91508.t1	A0A072VBF4	87.6	97	7	1	1	97	1	92	9.4e-36	159.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene91508	0.838503	2.77E-57	-1.69E-46
MS.gene049369	MS.gene91508	0.836001	1.21E-56	-1.69E-46
MS.gene049396	MS.gene91508	0.830358	3.07E-55	-1.69E-46
MS.gene049907	MS.gene91508	0.807217	5.63E-50	-1.69E-46
MS.gene050122	MS.gene91508	0.833101	6.47E-56	-1.69E-46
MS.gene050499	MS.gene91508	0.818471	1.93E-52	-1.69E-46
MS.gene050502	MS.gene91508	0.823344	1.46E-53	-1.69E-46
MS.gene050520	MS.gene91508	0.809867	1.53E-50	-1.69E-46
MS.gene050593	MS.gene91508	0.810583	1.07E-50	-1.69E-46
MS.gene050670	MS.gene91508	0.830055	3.64E-55	-1.69E-46
MS.gene050747	MS.gene91508	0.81299	3.21E-51	-1.69E-46
MS.gene050950	MS.gene91508	0.808467	3.05E-50	-1.69E-46
MS.gene05105	MS.gene91508	0.818667	1.74E-52	-1.69E-46
MS.gene051387	MS.gene91508	0.828647	8.00E-55	-1.69E-46
MS.gene051509	MS.gene91508	0.826012	3.43E-54	-1.69E-46
MS.gene051605	MS.gene91508	0.808153	3.56E-50	-1.69E-46
MS.gene052414	MS.gene91508	0.829871	4.04E-55	-1.69E-46
MS.gene052421	MS.gene91508	0.821194	4.60E-53	-1.69E-46
MS.gene053169	MS.gene91508	0.813038	3.13E-51	-1.69E-46
MS.gene053436	MS.gene91508	0.815084	1.11E-51	-1.69E-46
MS.gene053449	MS.gene91508	0.803771	2.97E-49	-1.69E-46
MS.gene053574	MS.gene91508	0.835802	1.36E-56	-1.69E-46
MS.gene053626	MS.gene91508	0.806613	7.55E-50	-1.69E-46
MS.gene053813	MS.gene91508	0.850313	1.84E-60	-1.69E-46
MS.gene053912	MS.gene91508	0.816657	4.94E-52	-1.69E-46
MS.gene05505	MS.gene91508	0.814059	1.87E-51	-1.69E-46
MS.gene055116	MS.gene91508	0.812835	3.47E-51	-1.69E-46
MS.gene055741	MS.gene91508	0.809225	2.10E-50	-1.69E-46
MS.gene055744	MS.gene91508	0.809361	1.97E-50	-1.69E-46
MS.gene055909	MS.gene91508	0.859922	2.96E-63	-1.69E-46
MS.gene055911	MS.gene91508	0.861629	8.97E-64	-1.69E-46
MS.gene056166	MS.gene91508	0.824478	7.90E-54	-1.69E-46
MS.gene056407	MS.gene91508	0.806715	7.19E-50	-1.69E-46
MS.gene056422	MS.gene91508	0.846386	2.24E-59	-1.69E-46
MS.gene056511	MS.gene91508	0.855964	4.43E-62	-1.69E-46
MS.gene056514	MS.gene91508	0.800218	1.60E-48	-1.69E-46
MS.gene05664	MS.gene91508	0.81337	2.65E-51	-1.69E-46
MS.gene05666	MS.gene91508	0.837957	3.83E-57	-1.69E-46
MS.gene057019	MS.gene91508	0.81779	2.75E-52	-1.69E-46
MS.gene057537	MS.gene91508	0.816278	6.00E-52	-1.69E-46
MS.gene057734	MS.gene91508	0.860834	1.57E-63	-1.69E-46
MS.gene057749	MS.gene91508	0.820156	7.96E-53	-1.69E-46
MS.gene057823	MS.gene91508	0.81429	1.66E-51	-1.69E-46
MS.gene058007	MS.gene91508	0.821913	3.13E-53	-1.69E-46
MS.gene058019	MS.gene91508	0.803611	3.21E-49	-1.69E-46
MS.gene058075	MS.gene91508	0.836808	7.53E-57	-1.69E-46
MS.gene058120	MS.gene91508	0.819346	1.22E-52	-1.69E-46
MS.gene058459	MS.gene91508	0.82472	6.93E-54	-1.69E-46
MS.gene058700	MS.gene91508	0.831983	1.22E-55	-1.69E-46
MS.gene058948	MS.gene91508	0.829997	3.76E-55	-1.69E-46
MS.gene058995	MS.gene91508	0.827509	1.50E-54	-1.69E-46
MS.gene059367	MS.gene91508	0.810371	1.19E-50	-1.69E-46
MS.gene059368	MS.gene91508	0.835695	1.44E-56	-1.69E-46
MS.gene059517	MS.gene91508	0.81029	1.24E-50	-1.69E-46
MS.gene059674	MS.gene91508	0.818388	2.01E-52	-1.69E-46
MS.gene059680	MS.gene91508	0.814408	1.56E-51	-1.69E-46
MS.gene059688	MS.gene91508	0.818177	2.25E-52	-1.69E-46
MS.gene059711	MS.gene91508	0.821199	4.58E-53	-1.69E-46
MS.gene059807	MS.gene91508	0.84224	2.92E-58	-1.69E-46
MS.gene059876	MS.gene91508	0.809159	2.17E-50	-1.69E-46
MS.gene059933	MS.gene91508	0.815499	8.96E-52	-1.69E-46
MS.gene060372	MS.gene91508	0.838311	3.10E-57	-1.69E-46
MS.gene060403	MS.gene91508	0.80143	9.04E-49	-1.69E-46
MS.gene060482	MS.gene91508	0.800171	1.63E-48	-1.69E-46
MS.gene060804	MS.gene91508	0.806686	7.29E-50	-1.69E-46
MS.gene060835	MS.gene91508	0.80344	3.48E-49	-1.69E-46
MS.gene060837	MS.gene91508	0.858583	7.46E-63	-1.69E-46
MS.gene06097	MS.gene91508	0.849632	2.86E-60	-1.69E-46
MS.gene061200	MS.gene91508	0.800718	1.26E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91508.t1	MTR_2g084565	88.000	100	7	1	1	100	1	95	2.17e-54	176

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAATAATTAGCTTATTAAA+TGG	0.232460	2.1:-16881861	None:intergenic
AATAAGCTAATTATTGAATC+AGG	0.247635	2.1:+16881867	MS.gene91508:CDS
GGAATCAGACGCGACGGTTT+CGG	0.372599	2.1:-16881748	None:intergenic
AGCTTATTAAATGGAGTCTC+TGG	0.400143	2.1:-16881852	None:intergenic
CATTTCACCGGCTTGAGCGC+CGG	0.483931	2.1:-16881684	None:intergenic
ATAAGCTAATTATTGAATCA+GGG	0.502968	2.1:+16881868	MS.gene91508:CDS
AAAGGTTGGAAGCGAAAGTG+AGG	0.504236	2.1:-16881558	None:intergenic
TTCATCATCAACAGCTTCAT+CGG	0.505527	2.1:-16881769	None:intergenic
TCGGTTTCAGCGTCGTTCCA+CGG	0.517395	2.1:-16881729	None:intergenic
ATCATCGCCGGCGCTCAAGC+CGG	0.529485	2.1:+16881677	MS.gene91508:CDS
GTGGATCTACTGCATTTCAC+CGG	0.543097	2.1:-16881696	None:intergenic
GTGAGAGATTCGTGGAATTG+TGG	0.544397	2.1:-16881624	None:intergenic
AATCTCTCACTCTTCTTCGC+CGG	0.546307	2.1:+16881637	MS.gene91508:CDS
CGGTGAGGTCGGAGAAGAAG+AGG	0.549065	2.1:-16881599	None:intergenic
GTCGGAGAAGAAGAGGTAGT+TGG	0.555331	2.1:-16881592	None:intergenic
TAGTTGGTGCGCGTGAGGAA+AGG	0.579052	2.1:-16881576	None:intergenic
AGATTCGTGGAATTGTGGTT+CGG	0.582093	2.1:-16881619	None:intergenic
TAAGCTAATTATTGAATCAG+GGG	0.582158	2.1:+16881869	MS.gene91508:CDS
GAGCGCCGGCGATGATGTAA+CGG	0.587494	2.1:-16881670	None:intergenic
GAATTGTGGTTCGGTGAGGT+CGG	0.590714	2.1:-16881610	None:intergenic
TCTGGAGATTCTCTATCGCT+CGG	0.591143	2.1:-16881834	None:intergenic
AGAGGTAGTTGGTGCGCGTG+AGG	0.591230	2.1:-16881581	None:intergenic
TGGTGCGCGTGAGGAAAGGT+TGG	0.598690	2.1:-16881572	None:intergenic
GAAGAAGAGTGAGAGATTCG+TGG	0.609323	2.1:-16881632	None:intergenic
AATCTCCGTTACATCATCGC+CGG	0.612622	2.1:+16881665	MS.gene91508:CDS
CGTGGAATTGTGGTTCGGTG+AGG	0.623398	2.1:-16881614	None:intergenic
TTCATCGGAATCAGACGCGA+CGG	0.634341	2.1:-16881754	None:intergenic
GCTGTTGATGATGAATCAAA+CGG	0.636421	2.1:+16881777	MS.gene91508:CDS
AAGCTAATTATTGAATCAGG+GGG	0.639720	2.1:+16881870	MS.gene91508:CDS
GGTGAAATGCAGTAGATCCA+CGG	0.652611	2.1:+16881698	MS.gene91508:CDS
GATCCACGGAAGAAAAGCCG+TGG	0.662772	2.1:+16881712	MS.gene91508:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCAATAATTAGCTTATTAAA+TGG	-	chr2.1:16881864-16881883	None:intergenic	15.0%
!!	AATAAGCTAATTATTGAATC+AGG	+	chr2.1:16881867-16881886	MS.gene91508:CDS	20.0%
!!	ATAAGCTAATTATTGAATCA+GGG	+	chr2.1:16881868-16881887	MS.gene91508:CDS	20.0%
!	TAAGCTAATTATTGAATCAG+GGG	+	chr2.1:16881869-16881888	MS.gene91508:CDS	25.0%
	AAGCTAATTATTGAATCAGG+GGG	+	chr2.1:16881870-16881889	MS.gene91508:CDS	30.0%
!	TCTATTGAAGAAGTTTTGCT+CGG	-	chr2.1:16881810-16881829	None:intergenic	30.0%
	AGCTTATTAAATGGAGTCTC+TGG	-	chr2.1:16881855-16881874	None:intergenic	35.0%
	TTCATCATCAACAGCTTCAT+CGG	-	chr2.1:16881772-16881791	None:intergenic	35.0%
!!	GCTGTTGATGATGAATCAAA+CGG	+	chr2.1:16881777-16881796	MS.gene91508:CDS	35.0%
	AGATTCGTGGAATTGTGGTT+CGG	-	chr2.1:16881622-16881641	None:intergenic	40.0%
!!	ATGTAACGGAGATTTTGCTC+CGG	-	chr2.1:16881659-16881678	None:intergenic	40.0%
	AAAGGTTGGAAGCGAAAGTG+AGG	-	chr2.1:16881561-16881580	None:intergenic	45.0%
	AATCTCCGTTACATCATCGC+CGG	+	chr2.1:16881665-16881684	MS.gene91508:CDS	45.0%
	AATCTCTCACTCTTCTTCGC+CGG	+	chr2.1:16881637-16881656	MS.gene91508:CDS	45.0%
	GAAGAAGAGTGAGAGATTCG+TGG	-	chr2.1:16881635-16881654	None:intergenic	45.0%
	GGTGAAATGCAGTAGATCCA+CGG	+	chr2.1:16881698-16881717	MS.gene91508:CDS	45.0%
	GTGAGAGATTCGTGGAATTG+TGG	-	chr2.1:16881627-16881646	None:intergenic	45.0%
	GTGGATCTACTGCATTTCAC+CGG	-	chr2.1:16881699-16881718	None:intergenic	45.0%
	TCTGGAGATTCTCTATCGCT+CGG	-	chr2.1:16881837-16881856	None:intergenic	45.0%
	GTCGGAGAAGAAGAGGTAGT+TGG	-	chr2.1:16881595-16881614	None:intergenic	50.0%
	TTCATCGGAATCAGACGCGA+CGG	-	chr2.1:16881757-16881776	None:intergenic	50.0%
!!	GAATTGTGGTTCGGTGAGGT+CGG	-	chr2.1:16881613-16881632	None:intergenic	50.0%
	GATCCACGGAAGAAAAGCCG+TGG	+	chr2.1:16881712-16881731	MS.gene91508:CDS	55.0%
	GGAATCAGACGCGACGGTTT+CGG	-	chr2.1:16881751-16881770	None:intergenic	55.0%
	TCGGTTTCAGCGTCGTTCCA+CGG	-	chr2.1:16881732-16881751	None:intergenic	55.0%
!	GTTCCACGGCTTTTCTTCCG+TGG	-	chr2.1:16881718-16881737	None:intergenic	55.0%
!	TAGTTGGTGCGCGTGAGGAA+AGG	-	chr2.1:16881579-16881598	None:intergenic	55.0%
!!	CGTGGAATTGTGGTTCGGTG+AGG	-	chr2.1:16881617-16881636	None:intergenic	55.0%
	CGGTGAGGTCGGAGAAGAAG+AGG	-	chr2.1:16881602-16881621	None:intergenic	60.0%
	TGGTGCGCGTGAGGAAAGGT+TGG	-	chr2.1:16881575-16881594	None:intergenic	60.0%
!	AGAGGTAGTTGGTGCGCGTG+AGG	-	chr2.1:16881584-16881603	None:intergenic	60.0%
!!	CATTTCACCGGCTTGAGCGC+CGG	-	chr2.1:16881687-16881706	None:intergenic	60.0%
!!	GAGCGCCGGCGATGATGTAA+CGG	-	chr2.1:16881673-16881692	None:intergenic	60.0%
	ATCATCGCCGGCGCTCAAGC+CGG	+	chr2.1:16881677-16881696	MS.gene91508:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	16881537	16881899	16881537	ID=MS.gene91508
chr2.1	mRNA	16881537	16881899	16881537	ID=MS.gene91508.t1;Parent=MS.gene91508
chr2.1	exon	16881537	16881899	16881537	ID=MS.gene91508.t1.exon1;Parent=MS.gene91508.t1
chr2.1	CDS	16881537	16881899	16881537	ID=cds.MS.gene91508.t1;Parent=MS.gene91508.t1

Gene Sequence	>MS.gene91508 ATGGCTGTAACTCTAACTTCTCCTCACTTTCGCTTCCAACCTTTCCTCACGCGCACCAACTACCTCTTCTTCTCCGACCTCACCGAACCACAATTCCACGAATCTCTCACTCTTCTTCGCCGGAGCAAAATCTCCGTTACATCATCGCCGGCGCTCAAGCCGGTGAAATGCAGTAGATCCACGGAAGAAAAGCCGTGGAACGACGCTGAAACCGAAACCGTCGCGTCTGATTCCGATGAAGCTGTTGATGATGAATCAAACGGTCAAGCTCCGAGCAAAACTTCTTCAATAGAGTCTCCGAGCGATAGAGAATCTCCAGAGACTCCATTTAATAAGCTAATTATTGAATCAGGGGGAAAATAG
Protein sequence	>MS.gene91508.t1 MAVTLTSPHFRFQPFLTRTNYLFFSDLTEPQFHESLTLLRRSKISVTSSPALKPVKCSRSTEEKPWNDAETETVASDSDEAVDDESNGQAPSKTSSIESPSDRESPETPFNKLIIESGGK