Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97019.t1 | XP_013449868.1 | 90.6 | 224 | 20 | 1 | 1 | 223 | 1 | 224 | 1.50E-109 | 405.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97019.t1 | Q9M2J7 | 80.5 | 123 | 22 | 1 | 3 | 123 | 6 | 128 | 1.7e-53 | 210.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97019.t1 | A0A072U3H8 | 90.6 | 224 | 20 | 1 | 1 | 223 | 1 | 224 | 1.1e-109 | 405.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene97019.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene97019 | -0.80332 | 3.69E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene97019 | -0.800671 | 1.29E-48 | -1.69E-46 |
| MS.gene049265 | MS.gene97019 | 0.81325 | 2.82E-51 | -1.69E-46 |
| MS.gene04932 | MS.gene97019 | 0.838387 | 2.96E-57 | -1.69E-46 |
| MS.gene049549 | MS.gene97019 | 0.801227 | 9.94E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene97019 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene050380 | MS.gene97019 | 0.819262 | 1.27E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene97019 | -0.803555 | 3.30E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene97019 | 0.821713 | 3.49E-53 | -1.69E-46 |
| MS.gene05081 | MS.gene97019 | -0.81569 | 8.12E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene97019 | -0.815342 | 9.71E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene97019 | -0.815131 | 1.08E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene97019 | 0.818159 | 2.27E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene97019 | 0.811917 | 5.51E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene97019 | -0.809069 | 2.27E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene97019 | -0.803202 | 3.90E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene97019 | -0.800461 | 1.43E-48 | -1.69E-46 |
| MS.gene051996 | MS.gene97019 | 0.802037 | 6.78E-49 | -1.69E-46 |
| MS.gene05234 | MS.gene97019 | 0.813633 | 2.32E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene97019 | 0.822943 | 1.81E-53 | -1.69E-46 |
| MS.gene05244 | MS.gene97019 | 0.800182 | 1.62E-48 | -1.69E-46 |
| MS.gene052606 | MS.gene97019 | 0.816591 | 5.11E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene97019 | -0.800092 | 1.69E-48 | -1.69E-46 |
| MS.gene052702 | MS.gene97019 | 0.808493 | 3.01E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene97019 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene053421 | MS.gene97019 | 0.825199 | 5.34E-54 | -1.69E-46 |
| MS.gene053479 | MS.gene97019 | -0.814553 | 1.45E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene97019 | -0.803166 | 3.97E-49 | -1.69E-46 |
| MS.gene054147 | MS.gene97019 | 0.804431 | 2.17E-49 | -1.69E-46 |
| MS.gene054290 | MS.gene97019 | 0.845816 | 3.21E-59 | -1.69E-46 |
| MS.gene054595 | MS.gene97019 | -0.813693 | 2.25E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene97019 | 0.834502 | 2.89E-56 | -1.69E-46 |
| MS.gene054742 | MS.gene97019 | 0.845397 | 4.17E-59 | -1.69E-46 |
| MS.gene055202 | MS.gene97019 | 0.819393 | 1.19E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene97019 | -0.826672 | 2.39E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene97019 | -0.811081 | 8.37E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene97019 | -0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene055941 | MS.gene97019 | -0.810201 | 1.30E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene97019 | 0.838592 | 2.62E-57 | -1.69E-46 |
| MS.gene056387 | MS.gene97019 | -0.814704 | 1.34E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene97019 | 0.836385 | 9.65E-57 | -1.69E-46 |
| MS.gene056591 | MS.gene97019 | 0.836 | 1.21E-56 | -1.69E-46 |
| MS.gene056688 | MS.gene97019 | 0.828606 | 8.19E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene97019 | 0.800188 | 1.62E-48 | -1.69E-46 |
| MS.gene056856 | MS.gene97019 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene056920 | MS.gene97019 | 0.819114 | 1.38E-52 | -1.69E-46 |
| MS.gene057544 | MS.gene97019 | 0.859197 | 4.89E-63 | -1.69E-46 |
| MS.gene058093 | MS.gene97019 | 0.816807 | 4.57E-52 | -1.69E-46 |
| MS.gene058157 | MS.gene97019 | -0.811787 | 5.88E-51 | -1.69E-46 |
| MS.gene058158 | MS.gene97019 | 0.827545 | 1.47E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene97019 | 0.801684 | 8.02E-49 | -1.69E-46 |
| MS.gene058177 | MS.gene97019 | 0.810421 | 1.16E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene97019 | -0.802098 | 6.59E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene97019 | -0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene058418 | MS.gene97019 | 0.814752 | 1.31E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene97019 | 0.80419 | 2.43E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene97019 | -0.805243 | 1.47E-49 | -1.69E-46 |
| MS.gene059037 | MS.gene97019 | 0.802953 | 4.39E-49 | -1.69E-46 |
| MS.gene059328 | MS.gene97019 | 0.80093 | 1.14E-48 | -1.69E-46 |
| MS.gene05934 | MS.gene97019 | -0.800599 | 1.34E-48 | -1.69E-46 |
| MS.gene059902 | MS.gene97019 | -0.803392 | 3.56E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene97019 | 0.82079 | 5.69E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97019.t1 | MTR_7g096610 | 96.875 | 224 | 6 | 1 | 1 | 223 | 1 | 224 | 1.09e-165 | 456 |
| MS.gene97019.t1 | MTR_1g070205 | 71.204 | 191 | 45 | 5 | 8 | 194 | 12 | 196 | 1.47e-92 | 271 |
| MS.gene97019.t1 | MTR_7g096530 | 73.585 | 106 | 28 | 0 | 3 | 108 | 9 | 114 | 3.19e-55 | 176 |
| MS.gene97019.t1 | MTR_5g080470 | 55.782 | 147 | 61 | 2 | 2 | 145 | 1 | 146 | 2.72e-52 | 167 |
| MS.gene97019.t1 | MTR_8g036085 | 50.000 | 176 | 65 | 3 | 8 | 162 | 23 | 196 | 1.02e-51 | 167 |
| MS.gene97019.t1 | MTR_1g070220 | 70.192 | 104 | 31 | 0 | 5 | 108 | 12 | 115 | 2.94e-49 | 160 |
| MS.gene97019.t1 | MTR_7g074990 | 71.287 | 101 | 29 | 0 | 8 | 108 | 20 | 120 | 3.57e-49 | 161 |
| MS.gene97019.t1 | MTR_8g036105 | 66.667 | 105 | 35 | 0 | 6 | 110 | 16 | 120 | 1.29e-48 | 159 |
| MS.gene97019.t1 | MTR_6g027710 | 63.889 | 108 | 39 | 0 | 7 | 114 | 39 | 146 | 1.05e-46 | 154 |
| MS.gene97019.t1 | MTR_7g075230 | 64.706 | 119 | 37 | 4 | 6 | 122 | 10 | 125 | 1.67e-45 | 150 |
| MS.gene97019.t1 | MTR_3g071590 | 43.919 | 148 | 80 | 2 | 1 | 148 | 1 | 145 | 2.80e-36 | 125 |
| MS.gene97019.t1 | MTR_5g083230 | 45.625 | 160 | 72 | 5 | 4 | 162 | 2 | 147 | 6.18e-36 | 125 |
| MS.gene97019.t1 | MTR_6g027700 | 55.446 | 101 | 45 | 0 | 8 | 108 | 7 | 107 | 4.66e-35 | 124 |
| MS.gene97019.t1 | MTR_4g083680 | 52.830 | 106 | 49 | 1 | 4 | 109 | 11 | 115 | 9.01e-34 | 119 |
| MS.gene97019.t1 | MTR_3g031660 | 44.516 | 155 | 79 | 6 | 4 | 157 | 2 | 150 | 1.02e-33 | 119 |
| MS.gene97019.t1 | MTR_7g033800 | 56.122 | 98 | 42 | 1 | 5 | 102 | 8 | 104 | 3.34e-33 | 118 |
| MS.gene97019.t1 | MTR_2g068760 | 55.000 | 100 | 44 | 1 | 4 | 103 | 9 | 107 | 7.84e-33 | 116 |
| MS.gene97019.t1 | MTR_5g083010 | 41.250 | 160 | 76 | 4 | 5 | 164 | 8 | 149 | 8.06e-33 | 117 |
| MS.gene97019.t1 | MTR_3g452660 | 50.877 | 114 | 55 | 1 | 2 | 115 | 1 | 113 | 9.65e-33 | 120 |
| MS.gene97019.t1 | MTR_4g105170 | 57.292 | 96 | 40 | 1 | 7 | 102 | 10 | 104 | 1.45e-32 | 117 |
| MS.gene97019.t1 | MTR_5g083960 | 53.398 | 103 | 45 | 2 | 7 | 108 | 38 | 138 | 1.68e-32 | 117 |
| MS.gene97019.t1 | MTR_3g077240 | 56.250 | 96 | 41 | 1 | 7 | 102 | 9 | 103 | 7.02e-32 | 115 |
| MS.gene97019.t1 | MTR_3g094690 | 55.446 | 101 | 42 | 2 | 7 | 106 | 46 | 144 | 1.13e-31 | 115 |
| MS.gene97019.t1 | MTR_5g017950 | 51.818 | 110 | 52 | 1 | 1 | 110 | 1 | 109 | 3.27e-31 | 116 |
| MS.gene97019.t1 | MTR_3g071420 | 49.515 | 103 | 51 | 1 | 5 | 107 | 11 | 112 | 5.03e-31 | 112 |
| MS.gene97019.t1 | MTR_6g005070 | 53.061 | 98 | 45 | 1 | 5 | 102 | 6 | 102 | 5.89e-31 | 111 |
| MS.gene97019.t1 | MTR_3g073690 | 48.182 | 110 | 56 | 1 | 1 | 110 | 2 | 110 | 1.28e-30 | 112 |
| MS.gene97019.t1 | MTR_3g094690 | 55.446 | 101 | 41 | 3 | 7 | 106 | 46 | 143 | 7.05e-30 | 111 |
| MS.gene97019.t1 | MTR_2g093310 | 51.546 | 97 | 46 | 1 | 7 | 103 | 32 | 127 | 8.63e-30 | 110 |
| MS.gene97019.t1 | MTR_6g005080 | 50.485 | 103 | 49 | 2 | 1 | 102 | 2 | 103 | 3.87e-29 | 106 |
| MS.gene97019.t1 | MTR_4g060950 | 53.061 | 98 | 44 | 2 | 7 | 103 | 23 | 119 | 2.42e-27 | 103 |
| MS.gene97019.t1 | MTR_5g075020 | 55.340 | 103 | 43 | 2 | 7 | 108 | 42 | 142 | 1.60e-26 | 102 |
| MS.gene97019.t1 | MTR_1g095850 | 51.579 | 95 | 45 | 1 | 9 | 103 | 73 | 166 | 2.13e-26 | 102 |
| MS.gene97019.t1 | MTR_5g075020 | 55.340 | 103 | 43 | 2 | 7 | 108 | 46 | 146 | 3.09e-26 | 101 |
| MS.gene97019.t1 | MTR_8g040900 | 55.172 | 87 | 38 | 1 | 2 | 88 | 1 | 86 | 3.17e-25 | 99.0 |
| MS.gene97019.t1 | MTR_5g093010 | 40.000 | 140 | 77 | 3 | 9 | 148 | 6 | 138 | 5.80e-25 | 95.9 |
| MS.gene97019.t1 | MTR_7g028905 | 43.269 | 104 | 58 | 1 | 7 | 110 | 4 | 106 | 3.10e-24 | 93.2 |
| MS.gene97019.t1 | MTR_8g079660 | 37.500 | 104 | 64 | 1 | 8 | 111 | 8 | 110 | 8.04e-19 | 82.8 |
| MS.gene97019.t1 | MTR_8g079620 | 40.385 | 104 | 61 | 1 | 8 | 111 | 8 | 110 | 1.37e-18 | 81.6 |
| MS.gene97019.t1 | MTR_8g017090 | 37.255 | 102 | 63 | 1 | 9 | 110 | 13 | 113 | 4.82e-18 | 81.3 |
| MS.gene97019.t1 | MTR_4g107450 | 37.143 | 105 | 65 | 1 | 4 | 108 | 6 | 109 | 1.34e-17 | 79.7 |
| MS.gene97019.t1 | MTR_2g100020 | 34.579 | 107 | 69 | 1 | 4 | 110 | 15 | 120 | 2.43e-17 | 79.0 |
| MS.gene97019.t1 | MTR_6g011250 | 38.636 | 88 | 52 | 2 | 2 | 88 | 3 | 89 | 1.81e-15 | 72.0 |
| MS.gene97019.t1 | MTR_6g011200 | 42.353 | 85 | 45 | 3 | 9 | 90 | 10 | 93 | 3.90e-15 | 72.4 |
| MS.gene97019.t1 | MTR_8g079580 | 35.294 | 102 | 64 | 2 | 8 | 108 | 8 | 108 | 8.90e-15 | 71.2 |
| MS.gene97019.t1 | MTR_6g011230 | 34.906 | 106 | 65 | 2 | 9 | 111 | 12 | 116 | 1.55e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97019.t1 | AT3G58190 | 81.356 | 118 | 20 | 1 | 8 | 123 | 11 | 128 | 1.05e-70 | 215 |
| MS.gene97019.t1 | AT2G42440 | 74.046 | 131 | 32 | 1 | 2 | 130 | 1 | 131 | 1.03e-68 | 211 |
| MS.gene97019.t1 | AT2G31310 | 61.972 | 142 | 53 | 1 | 2 | 143 | 1 | 141 | 5.62e-60 | 187 |
| MS.gene97019.t1 | AT2G42430 | 72.897 | 107 | 29 | 0 | 4 | 110 | 11 | 117 | 5.61e-54 | 173 |
| MS.gene97019.t1 | AT4G00210 | 58.333 | 132 | 54 | 1 | 3 | 133 | 6 | 137 | 2.18e-51 | 166 |
| MS.gene97019.t1 | AT3G03760 | 71.569 | 102 | 29 | 0 | 7 | 108 | 50 | 151 | 5.03e-50 | 164 |
| MS.gene97019.t1 | AT5G06080 | 68.224 | 107 | 34 | 0 | 2 | 108 | 1 | 107 | 1.05e-49 | 160 |
| MS.gene97019.t1 | AT2G45420 | 74.000 | 100 | 26 | 0 | 11 | 110 | 40 | 139 | 2.77e-49 | 162 |
| MS.gene97019.t1 | AT4G00220 | 70.000 | 100 | 30 | 0 | 11 | 110 | 20 | 119 | 1.78e-46 | 154 |
| MS.gene97019.t1 | AT2G45410 | 60.000 | 115 | 43 | 2 | 11 | 122 | 19 | 133 | 5.15e-42 | 141 |
| MS.gene97019.t1 | AT3G27650 | 57.292 | 96 | 40 | 1 | 7 | 102 | 38 | 132 | 5.82e-33 | 117 |
| MS.gene97019.t1 | AT2G30130 | 51.695 | 118 | 53 | 3 | 2 | 118 | 1 | 115 | 1.50e-32 | 117 |
| MS.gene97019.t1 | AT5G63090 | 40.642 | 187 | 88 | 6 | 7 | 193 | 10 | 173 | 2.49e-32 | 116 |
| MS.gene97019.t1 | AT5G63090 | 40.642 | 187 | 88 | 6 | 7 | 193 | 10 | 173 | 2.49e-32 | 116 |
| MS.gene97019.t1 | AT5G63090 | 40.642 | 187 | 88 | 6 | 7 | 193 | 10 | 173 | 2.49e-32 | 116 |
| MS.gene97019.t1 | AT5G63090 | 40.642 | 187 | 88 | 6 | 7 | 193 | 10 | 173 | 2.49e-32 | 116 |
| MS.gene97019.t1 | AT1G07900 | 53.608 | 97 | 44 | 1 | 7 | 103 | 32 | 127 | 1.01e-30 | 112 |
| MS.gene97019.t1 | AT3G11090 | 50.980 | 102 | 49 | 1 | 5 | 106 | 8 | 108 | 1.14e-30 | 111 |
| MS.gene97019.t1 | AT1G31320 | 53.261 | 92 | 42 | 1 | 4 | 95 | 9 | 99 | 1.21e-30 | 111 |
| MS.gene97019.t1 | AT1G16530 | 48.305 | 118 | 58 | 3 | 7 | 122 | 13 | 129 | 2.33e-30 | 110 |
| MS.gene97019.t1 | AT2G28500 | 53.608 | 97 | 44 | 1 | 7 | 103 | 54 | 149 | 6.11e-30 | 111 |
| MS.gene97019.t1 | AT5G66870 | 51.376 | 109 | 52 | 1 | 2 | 110 | 1 | 108 | 1.54e-29 | 112 |
| MS.gene97019.t1 | AT2G30340 | 51.456 | 103 | 47 | 2 | 7 | 108 | 51 | 151 | 5.28e-29 | 109 |
| MS.gene97019.t1 | AT2G30340 | 51.456 | 103 | 47 | 2 | 7 | 108 | 52 | 152 | 5.62e-29 | 109 |
| MS.gene97019.t1 | AT2G23660 | 49.020 | 102 | 51 | 1 | 7 | 108 | 4 | 104 | 7.40e-27 | 105 |
| MS.gene97019.t1 | AT2G23660 | 49.020 | 102 | 51 | 1 | 7 | 108 | 4 | 104 | 7.40e-27 | 105 |
| MS.gene97019.t1 | AT2G23660 | 49.020 | 102 | 51 | 1 | 7 | 108 | 4 | 104 | 7.40e-27 | 105 |
| MS.gene97019.t1 | AT3G26660 | 46.316 | 95 | 50 | 1 | 9 | 103 | 6 | 99 | 3.63e-26 | 98.6 |
| MS.gene97019.t1 | AT1G65620 | 56.977 | 86 | 36 | 1 | 3 | 88 | 4 | 88 | 6.14e-26 | 100 |
| MS.gene97019.t1 | AT1G65620 | 56.977 | 86 | 36 | 1 | 3 | 88 | 4 | 88 | 6.14e-26 | 100 |
| MS.gene97019.t1 | AT1G65620 | 56.977 | 86 | 36 | 1 | 3 | 88 | 4 | 88 | 6.14e-26 | 100 |
| MS.gene97019.t1 | AT1G65620 | 56.977 | 86 | 36 | 1 | 3 | 88 | 4 | 88 | 6.14e-26 | 100 |
| MS.gene97019.t1 | AT1G65620 | 56.977 | 86 | 36 | 1 | 3 | 88 | 4 | 88 | 6.14e-26 | 100 |
| MS.gene97019.t1 | AT3G26620 | 46.316 | 95 | 50 | 1 | 9 | 103 | 6 | 99 | 6.97e-26 | 97.8 |
| MS.gene97019.t1 | AT2G40470 | 54.369 | 103 | 44 | 2 | 7 | 108 | 19 | 119 | 9.38e-26 | 99.8 |
| MS.gene97019.t1 | AT2G40470 | 54.369 | 103 | 44 | 2 | 7 | 108 | 44 | 144 | 1.32e-25 | 99.8 |
| MS.gene97019.t1 | AT3G50510 | 42.478 | 113 | 64 | 1 | 2 | 114 | 6 | 117 | 4.09e-25 | 97.8 |
| MS.gene97019.t1 | AT3G50510 | 42.478 | 113 | 64 | 1 | 2 | 114 | 6 | 117 | 4.09e-25 | 97.8 |
| MS.gene97019.t1 | AT3G13850 | 38.136 | 118 | 63 | 3 | 9 | 118 | 37 | 152 | 1.28e-18 | 82.4 |
| MS.gene97019.t1 | AT1G72980 | 36.190 | 105 | 66 | 1 | 4 | 108 | 9 | 112 | 2.33e-16 | 75.1 |
| MS.gene97019.t1 | AT5G35900 | 38.235 | 102 | 62 | 1 | 9 | 110 | 6 | 106 | 6.68e-16 | 73.9 |
| MS.gene97019.t1 | AT3G47870 | 32.381 | 105 | 70 | 1 | 4 | 108 | 32 | 135 | 2.94e-14 | 70.9 |
| MS.gene97019.t1 | AT1G06280 | 38.667 | 75 | 45 | 1 | 9 | 83 | 25 | 98 | 4.22e-13 | 66.2 |
Find 47 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACTTCAAGATCCAATTTA+TGG | 0.151814 | 7.4:+12898396 | MS.gene97019:CDS |
| TTTCCAAAGTTCTTCTCATT+AGG | 0.257962 | 7.4:-12899004 | None:intergenic |
| CCTTATGAATAGCTGCAAAA+TGG | 0.260543 | 7.4:-12898278 | None:intergenic |
| ATTAGTGGAAGAAGAGAAAT+CGG | 0.262535 | 7.4:-12899104 | None:intergenic |
| GCAGCTATTCATAAGGTCTT+TGG | 0.287235 | 7.4:+12898285 | MS.gene97019:CDS |
| TGGGCAACACATCCATAAAT+TGG | 0.288880 | 7.4:-12898408 | None:intergenic |
| TCAACAACACAATATTATAA+TGG | 0.305675 | 7.4:+12899133 | MS.gene97019:CDS |
| TTCCAAAGTTCTTCTCATTA+GGG | 0.315481 | 7.4:-12899003 | None:intergenic |
| CTCATGGATCTAAACCCTAT+TGG | 0.344271 | 7.4:+12899169 | MS.gene97019:CDS |
| ATTGGAAACTATGAAAATTC+AGG | 0.345302 | 7.4:+12899187 | MS.gene97019:CDS |
| TTTCCTCCTCAAGAATTTGC+AGG | 0.357584 | 7.4:-12898202 | None:intergenic |
| ATGAGATGCTTTCTTCCAAT+AGG | 0.363922 | 7.4:-12899213 | None:intergenic |
| TGAAAATTCAGGAACCCTAT+TGG | 0.376897 | 7.4:+12899198 | MS.gene97019:CDS |
| ACGGCCATGATGACTGGTTC+TGG | 0.395558 | 7.4:+12898165 | None:intergenic |
| CAACAACACAATATTATAAT+GGG | 0.395664 | 7.4:+12899134 | MS.gene97019:CDS |
| TTGAGGAGGAAATGTGTTAG+AGG | 0.402110 | 7.4:+12898213 | MS.gene97019:CDS |
| TGAGATGCTTTCTTCCAATA+GGG | 0.428947 | 7.4:-12899212 | None:intergenic |
| TCTTGAGGGAGAATATTAGT+AGG | 0.429657 | 7.4:-12899028 | None:intergenic |
| GAGATGAACAAACCTGTTGC+TGG | 0.430997 | 7.4:-12898443 | None:intergenic |
| CTTATGAATAGCTGCAAAAT+GGG | 0.434815 | 7.4:-12898277 | None:intergenic |
| AACCCTAATGAGAAGAACTT+TGG | 0.435631 | 7.4:+12899001 | MS.gene97019:CDS |
| ATTCTTACGGCCATGATGAC+TGG | 0.446907 | 7.4:+12898159 | None:intergenic |
| AATGGGTAGCACCTTGTTCA+TGG | 0.448967 | 7.4:-12898260 | None:intergenic |
| TGTTGTTGATGAGAGATTAG+TGG | 0.463491 | 7.4:-12899119 | None:intergenic |
| TCCCTCAAGATCTTCAAAGT+TGG | 0.463884 | 7.4:+12899041 | MS.gene97019:CDS |
| CAAATTAGCCACTTACTGAT+AGG | 0.482945 | 7.4:-12899302 | None:intergenic |
| ACTGGTTCTGGTTCTCCTTG+TGG | 0.494488 | 7.4:+12898177 | MS.gene97019:CDS |
| TGGGACTACCACTACTCTCA+TGG | 0.512301 | 7.4:+12899153 | MS.gene97019:CDS |
| TCTTCAAAGTTGGTTCCAGA+TGG | 0.523638 | 7.4:+12899051 | MS.gene97019:CDS |
| CTGTCTTTGCATGTTGTCAA+AGG | 0.524030 | 7.4:-12899275 | None:intergenic |
| CCTTGTTCATGGCAAAAGTA+AGG | 0.524451 | 7.4:-12898249 | None:intergenic |
| AACCAACTTTGAAGATCTTG+AGG | 0.528680 | 7.4:-12899043 | None:intergenic |
| GCTTCACAACGATCAGTCAC+AGG | 0.531664 | 7.4:-12898342 | None:intergenic |
| TTAGTGGAAGAAGAGAAATC+GGG | 0.531706 | 7.4:-12899103 | None:intergenic |
| ACCAACTTTGAAGATCTTGA+GGG | 0.534056 | 7.4:-12899042 | None:intergenic |
| GTGGAGCCTGCAAATTCTTG+AGG | 0.534107 | 7.4:+12898196 | MS.gene97019:CDS |
| AAGAAATTGAGAACACATGT+TGG | 0.541417 | 7.4:-12899080 | None:intergenic |
| ATTATTACATCGCTCATCAA+AGG | 0.565424 | 7.4:-12899245 | None:intergenic |
| AGAACCAGAACCAGTCATCA+TGG | 0.567393 | 7.4:-12898169 | None:intergenic |
| TGAGAGAACAAGCAGCTCAA+AGG | 0.584503 | 7.4:+12898954 | MS.gene97019:CDS |
| AAGAATTTGCAGGCTCCACA+AGG | 0.586151 | 7.4:-12898192 | None:intergenic |
| CAGTCACAGGGAGGTGTGCG+AGG | 0.596818 | 7.4:-12898329 | None:intergenic |
| GTTTAGATCCATGAGAGTAG+TGG | 0.619212 | 7.4:-12899161 | None:intergenic |
| GAGCCTGCAAATTCTTGAGG+AGG | 0.637179 | 7.4:+12898199 | MS.gene97019:CDS |
| CTTCACAACGATCAGTCACA+GGG | 0.652040 | 7.4:-12898341 | None:intergenic |
| TTGACAACATGCAAAGACAG+TGG | 0.686531 | 7.4:+12899278 | MS.gene97019:CDS |
| CACAACGATCAGTCACAGGG+AGG | 0.754403 | 7.4:-12898338 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTCTTTTTTATATATAAAC+TGG | - | chr7.4:12898581-12898600 | None:intergenic | 15.0% |
| !!! | TTGAGTTTGTAATAAAATTT+TGG | + | chr7.4:12898809-12898828 | MS.gene97019:intron | 15.0% |
| !! | CAACAACACAATATTATAAT+GGG | + | chr7.4:12899134-12899153 | MS.gene97019:CDS | 20.0% |
| !! | TATATTTGATAAAATCACGA+TGG | + | chr7.4:12898701-12898720 | MS.gene97019:intron | 20.0% |
| !! | TCAACAACACAATATTATAA+TGG | + | chr7.4:12899133-12899152 | MS.gene97019:CDS | 20.0% |
| !!! | AATTTTCATAGTTTCCAATA+GGG | - | chr7.4:12899186-12899205 | None:intergenic | 20.0% |
| !!! | ATTAATTAACAAGAGTATCA+AGG | + | chr7.4:12898550-12898569 | MS.gene97019:intron | 20.0% |
| !!! | TTAATTAACAAGAGTATCAA+GGG | + | chr7.4:12898551-12898570 | MS.gene97019:intron | 20.0% |
| ! | ATTGGAAACTATGAAAATTC+AGG | + | chr7.4:12899187-12899206 | MS.gene97019:CDS | 25.0% |
| !! | GAATTTTCATAGTTTCCAAT+AGG | - | chr7.4:12899187-12899206 | None:intergenic | 25.0% |
| !! | TATGTCATGTTGATTGTATT+AGG | - | chr7.4:12898633-12898652 | None:intergenic | 25.0% |
| !!! | AGGTCATACTATTGATTTTT+TGG | - | chr7.4:12898613-12898632 | None:intergenic | 25.0% |
| AAGAAATTGAGAACACATGT+TGG | - | chr7.4:12899083-12899102 | None:intergenic | 30.0% | |
| AGACTTCAAGATCCAATTTA+TGG | + | chr7.4:12898396-12898415 | MS.gene97019:CDS | 30.0% | |
| ATTAGTGGAAGAAGAGAAAT+CGG | - | chr7.4:12899107-12899126 | None:intergenic | 30.0% | |
| ATTATTACATCGCTCATCAA+AGG | - | chr7.4:12899248-12899267 | None:intergenic | 30.0% | |
| CTTATGAATAGCTGCAAAAT+GGG | - | chr7.4:12898280-12898299 | None:intergenic | 30.0% | |
| TTCCAAAGTTCTTCTCATTA+GGG | - | chr7.4:12899006-12899025 | None:intergenic | 30.0% | |
| TTTCCAAAGTTCTTCTCATT+AGG | - | chr7.4:12899007-12899026 | None:intergenic | 30.0% | |
| ! | ATTAGGGTTTTCAGAATTTG+GGG | - | chr7.4:12898990-12899009 | None:intergenic | 30.0% |
| ! | CATTAGGGTTTTCAGAATTT+GGG | - | chr7.4:12898991-12899010 | None:intergenic | 30.0% |
| ! | TCATTAGGGTTTTCAGAATT+TGG | - | chr7.4:12898992-12899011 | None:intergenic | 30.0% |
| AACCCTAATGAGAAGAACTT+TGG | + | chr7.4:12899001-12899020 | MS.gene97019:CDS | 35.0% | |
| AATGTAAGCGTATACCATCT+AGG | - | chr7.4:12898490-12898509 | None:intergenic | 35.0% | |
| ATGAGATGCTTTCTTCCAAT+AGG | - | chr7.4:12899216-12899235 | None:intergenic | 35.0% | |
| ATGTAAGCGTATACCATCTA+GGG | - | chr7.4:12898489-12898508 | None:intergenic | 35.0% | |
| ATTCGCGCAGTTAATACAAT+TGG | + | chr7.4:12898766-12898785 | MS.gene97019:intron | 35.0% | |
| CCTTATGAATAGCTGCAAAA+TGG | - | chr7.4:12898281-12898300 | None:intergenic | 35.0% | |
| CTTAAACTGAAAACCCTAGA+TGG | + | chr7.4:12898473-12898492 | MS.gene97019:intron | 35.0% | |
| TCTTGAGGGAGAATATTAGT+AGG | - | chr7.4:12899031-12899050 | None:intergenic | 35.0% | |
| TGAAAATTCAGGAACCCTAT+TGG | + | chr7.4:12899198-12899217 | MS.gene97019:CDS | 35.0% | |
| TGAGATGCTTTCTTCCAATA+GGG | - | chr7.4:12899215-12899234 | None:intergenic | 35.0% | |
| TGATAAAATCACGATGGCAT+CGG | + | chr7.4:12898707-12898726 | MS.gene97019:intron | 35.0% | |
| TGTAAGACTTGGCACATATT+TGG | + | chr7.4:12898668-12898687 | MS.gene97019:intron | 35.0% | |
| TTAGTGGAAGAAGAGAAATC+GGG | - | chr7.4:12899106-12899125 | None:intergenic | 35.0% | |
| TTGCTGGAGTGCAAAAATAT+GGG | - | chr7.4:12898430-12898449 | None:intergenic | 35.0% | |
| TTGGTGTATTATTGATGCAC+TGG | + | chr7.4:12898785-12898804 | MS.gene97019:intron | 35.0% | |
| TTGTATTAACTGCGCGAATT+TGG | - | chr7.4:12898765-12898784 | None:intergenic | 35.0% | |
| ! | AACCAACTTTGAAGATCTTG+AGG | - | chr7.4:12899046-12899065 | None:intergenic | 35.0% |
| ! | ACATGTTGGAATTTTCCATC+TGG | - | chr7.4:12899069-12899088 | None:intergenic | 35.0% |
| ! | ACCAACTTTGAAGATCTTGA+GGG | - | chr7.4:12899045-12899064 | None:intergenic | 35.0% |
| ! | CCATTTTGCAGCTATTCATA+AGG | + | chr7.4:12898278-12898297 | MS.gene97019:CDS | 35.0% |
| ! | TGTTGTTGATGAGAGATTAG+TGG | - | chr7.4:12899122-12899141 | None:intergenic | 35.0% |
| ATAAAATCACGATGGCATCG+GGG | + | chr7.4:12898709-12898728 | MS.gene97019:intron | 40.0% | |
| ATTGACTACCTGCAAAGATG+AGG | - | chr7.4:12898912-12898931 | None:intergenic | 40.0% | |
| CCTTGTTCATGGCAAAAGTA+AGG | - | chr7.4:12898252-12898271 | None:intergenic | 40.0% | |
| CTCATGGATCTAAACCCTAT+TGG | + | chr7.4:12899169-12899188 | MS.gene97019:CDS | 40.0% | |
| CTGTCTTTGCATGTTGTCAA+AGG | - | chr7.4:12899278-12899297 | None:intergenic | 40.0% | |
| GATAAAATCACGATGGCATC+GGG | + | chr7.4:12898708-12898727 | MS.gene97019:intron | 40.0% | |
| GCAGCTATTCATAAGGTCTT+TGG | + | chr7.4:12898285-12898304 | MS.gene97019:CDS | 40.0% | |
| GTTGCTGGAGTGCAAAAATA+TGG | - | chr7.4:12898431-12898450 | None:intergenic | 40.0% | |
| GTTTAGATCCATGAGAGTAG+TGG | - | chr7.4:12899164-12899183 | None:intergenic | 40.0% | |
| TCCCTCAAGATCTTCAAAGT+TGG | + | chr7.4:12899041-12899060 | MS.gene97019:CDS | 40.0% | |
| TGGGCAACACATCCATAAAT+TGG | - | chr7.4:12898411-12898430 | None:intergenic | 40.0% | |
| TTCTTCTTCCTCATCTTTGC+AGG | + | chr7.4:12898901-12898920 | MS.gene97019:intron | 40.0% | |
| TTGACAACATGCAAAGACAG+TGG | + | chr7.4:12899278-12899297 | MS.gene97019:CDS | 40.0% | |
| TTGAGGAGGAAATGTGTTAG+AGG | + | chr7.4:12898213-12898232 | MS.gene97019:CDS | 40.0% | |
| TTTCCTCCTCAAGAATTTGC+AGG | - | chr7.4:12898205-12898224 | None:intergenic | 40.0% | |
| ! | TCTTCAAAGTTGGTTCCAGA+TGG | + | chr7.4:12899051-12899070 | MS.gene97019:CDS | 40.0% |
| !! | CCTTACTTTTGCCATGAACA+AGG | + | chr7.4:12898249-12898268 | MS.gene97019:CDS | 40.0% |
| !! | TATTTTTGCACTCCAGCAAC+AGG | + | chr7.4:12898431-12898450 | MS.gene97019:CDS | 40.0% |
| AAGAATTTGCAGGCTCCACA+AGG | - | chr7.4:12898195-12898214 | None:intergenic | 45.0% | |
| AATGGGTAGCACCTTGTTCA+TGG | - | chr7.4:12898263-12898282 | None:intergenic | 45.0% | |
| CCAAGTCTTACAGATCGACA+TGG | - | chr7.4:12898660-12898679 | None:intergenic | 45.0% | |
| CCATGTCGATCTGTAAGACT+TGG | + | chr7.4:12898657-12898676 | MS.gene97019:intron | 45.0% | |
| CTTCACAACGATCAGTCACA+GGG | - | chr7.4:12898344-12898363 | None:intergenic | 45.0% | |
| GAGATGAACAAACCTGTTGC+TGG | - | chr7.4:12898446-12898465 | None:intergenic | 45.0% | |
| TGAGAGAACAAGCAGCTCAA+AGG | + | chr7.4:12898954-12898973 | MS.gene97019:CDS | 45.0% | |
| ! | CACTGTGTTGATCTATGCAG+TGG | + | chr7.4:12898856-12898875 | MS.gene97019:intron | 45.0% |
| GAGCCTGCAAATTCTTGAGG+AGG | + | chr7.4:12898199-12898218 | MS.gene97019:CDS | 50.0% | |
| GCTTCACAACGATCAGTCAC+AGG | - | chr7.4:12898345-12898364 | None:intergenic | 50.0% | |
| GTGGAGCCTGCAAATTCTTG+AGG | + | chr7.4:12898196-12898215 | MS.gene97019:CDS | 50.0% | |
| TGGGACTACCACTACTCTCA+TGG | + | chr7.4:12899153-12899172 | MS.gene97019:CDS | 50.0% | |
| !! | ACTGGTTCTGGTTCTCCTTG+TGG | + | chr7.4:12898177-12898196 | MS.gene97019:CDS | 50.0% |
| CACAACGATCAGTCACAGGG+AGG | - | chr7.4:12898341-12898360 | None:intergenic | 55.0% | |
| ! | CAGTCACAGGGAGGTGTGCG+AGG | - | chr7.4:12898332-12898351 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 12898171 | 12899312 | 12898171 | ID=MS.gene97019 |
| chr7.4 | mRNA | 12898171 | 12899312 | 12898171 | ID=MS.gene97019.t1;Parent=MS.gene97019 |
| chr7.4 | exon | 12898171 | 12898452 | 12898171 | ID=MS.gene97019.t1.exon1;Parent=MS.gene97019.t1 |
| chr7.4 | CDS | 12898171 | 12898452 | 12898171 | ID=cds.MS.gene97019.t1;Parent=MS.gene97019.t1 |
| chr7.4 | exon | 12898923 | 12899312 | 12898923 | ID=MS.gene97019.t1.exon2;Parent=MS.gene97019.t1 |
| chr7.4 | CDS | 12898923 | 12899312 | 12898923 | ID=cds.MS.gene97019.t1;Parent=MS.gene97019.t1 |
| Gene Sequence |
| Protein sequence |
