AlfalfaGEDB | MsG0080048669.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048669.01

KEGG: -
GO: GO:0005524
PFAM: PF00004

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048669.01.T01	XP_003604901.1	98.485	462	7	0	2	463	15	476	0	944

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048669.01.T01	Q7X9A0	82.833	466	70	6	3	463	16	476	0	789

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048669.01.T01	G7JTD2	98.485	462	7	0	2	463	15	476	0.0	944

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048669.01	MsG0080049062.01	0.832479	9.236312e-56	1.033612e-52
MsG0080048669.01	MsG0180000467.01	0.800345	1.505296e-48	6.992777e-46
MsG0080048669.01	MsG0180003985.01	0.820815	5.620460e-53	4.493964e-50
MsG0080048669.01	MsG0180004553.01	0.800292	1.543544e-48	7.160671e-46
MsG0080048669.01	MsG0280006452.01	0.800595	1.338704e-48	6.258157e-46
MsG0080048669.01	MsG0280007247.01	0.806560	7.752508e-50	4.221052e-47
MsG0080048669.01	MsG0280009541.01	0.844649	6.635443e-59	1.078307e-55
MsG0080048669.01	MsG0280009579.01	0.802809	4.702967e-49	2.325798e-46
MsG0080048669.01	MsG0280009776.01	0.801424	9.063858e-49	4.326969e-46
MsG0080048669.01	MsG0380012065.01	0.813589	2.371118e-51	1.554828e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048669.01.T01	MTR_4g021210	98.485	462	7	0	2	463	15	476	0.0	944
MsG0080048669.01.T01	MTR_5g080450	83.514	461	75	1	3	463	16	475	0.0	820
MsG0080048669.01.T01	MTR_3g068030	84.086	421	65	2	5	424	17	436	0.0	738
MsG0080048669.01.T01	MTR_3g068030	84.086	421	65	2	5	424	3	422	0.0	736
MsG0080048669.01.T01	MTR_2g105480	53.767	292	128	6	121	406	123	413	5.24e-109	329
MsG0080048669.01.T01	MTR_2g105480	60.262	229	87	4	121	346	123	350	2.88e-97	296
MsG0080048669.01.T01	MTR_2g105480	65.730	178	57	4	121	295	123	299	9.87e-77	243
MsG0080048669.01.T01	MTR_0373s0020	96.429	28	1	0	426	453	11	38	2.86e-12	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048669.01.T01	AT2G39730	79.437	462	91	2	3	463	16	474	0.0	769
MsG0080048669.01.T01	AT2G39730	78.472	432	89	2	3	433	16	444	0.0	707
MsG0080048669.01.T01	AT2G39730	79.343	426	84	2	3	427	16	438	0.0	705
MsG0080048669.01.T01	AT1G73110	51.724	319	145	7	94	406	15	330	1.80e-110	330
MsG0080048669.01.T01	AT1G73110	52.564	312	139	7	101	406	119	427	5.58e-109	329

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Find 127 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAACAAGAGAATGTGAAG+AGG	0.574498	contig397end:-29455	MsG0080048669.01.T01:CDS
AAGACTTGTGAATTCAAAAG+AGG	0.535398	contig397end:-29547	MsG0080048669.01.T01:CDS
AAGATTGTCGACACATTCCC+TGG	0.416886	contig397end:-30063	MsG0080048669.01.T01:CDS
AAGGGTCAGCGCAACCTTCT+GGG	0.410738	contig397end:+29182	None:intergenic
AATAATGATGCTATCAAGAC+TGG	0.465950	contig397end:-29384	MsG0080048669.01.T01:CDS
AATATGGACAACACAGTTGA+TGG	0.442941	contig397end:-30874	MsG0080048669.01.T01:CDS
AATGATCTTGATGCAGGAGC+TGG	0.538362	contig397end:-30375	MsG0080048669.01.T01:CDS
AATGATGAGCGCCGGAGAAT+TGG	0.362682	contig397end:-30496	MsG0080048669.01.T01:CDS
AATGCACCTTACATAAAATA+TGG	0.337542	contig397end:+29015	None:intergenic
ACAAAATATACAACCTAAAG+TGG	0.462806	contig397end:+28985	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATCATCAGAATGAATC+GGG	+	contig397end:29438-29457	None:intergenic	30.0%
AACTCTTTGATCTACTTCTT+TGG	-	contig397end:30060-30079	MsG0080048669.01.T01:CDS	30.0%
AAGACTTGTGAATTCAAAAG+AGG	-	contig397end:31045-31064	MsG0080048669.01.T01:intron	30.0%
AATAATGATGCTATCAAGAC+TGG	-	contig397end:31208-31227	MsG0080048669.01.T01:intron	30.0%
ACGCATGGAAAAATTCTATT+GGG	-	contig397end:30429-30448	MsG0080048669.01.T01:CDS	30.0%
AGACTTGTGAATTCAAAAGA+GGG	-	contig397end:31046-31065	MsG0080048669.01.T01:intron	30.0%
CAACAATCATCAGAATGAAT+CGG	+	contig397end:29439-29458	None:intergenic	30.0%
CAAGTTTCAAACTCATATCA+CGG	+	contig397end:30897-30916	None:intergenic	30.0%
GGCAAAAATTGACTATAATG+AGG	-	contig397end:29034-29053	MsG0080048669.01.T01:three_prime_UTR	30.0%
TCCTATGATTATATCAGTAC+CGG	-	contig397end:29200-29219	MsG0080048669.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
contig397end	gene	28903	31711	28903	ID=MsG0080048669.01;Name=MsG0080048669.01
contig397end	mRNA	28903	31711	28903	ID=MsG0080048669.01.T01;Parent=MsG0080048669.01;Name=MsG0080048669.01.T01;_AED=0.29;_eAED=0.29;_QI=0\|1\|0.8\|1\|1\|1\|5\|217\|463
contig397end	exon	28903	29235	28903	ID=MsG0080048669.01.T01:exon:10181;Parent=MsG0080048669.01.T01
contig397end	exon	29373	29654	29373	ID=MsG0080048669.01.T01:exon:10180;Parent=MsG0080048669.01.T01
contig397end	exon	30052	30524	30052	ID=MsG0080048669.01.T01:exon:10179;Parent=MsG0080048669.01.T01
contig397end	exon	30709	30900	30709	ID=MsG0080048669.01.T01:exon:10178;Parent=MsG0080048669.01.T01
contig397end	exon	31383	31711	31383	ID=MsG0080048669.01.T01:exon:10177;Parent=MsG0080048669.01.T01
contig397end	CDS	31383	31711	31383	ID=MsG0080048669.01.T01:cds;Parent=MsG0080048669.01.T01
contig397end	CDS	30709	30900	30709	ID=MsG0080048669.01.T01:cds;Parent=MsG0080048669.01.T01
contig397end	CDS	30052	30524	30052	ID=MsG0080048669.01.T01:cds;Parent=MsG0080048669.01.T01
contig397end	CDS	29373	29654	29373	ID=MsG0080048669.01.T01:cds;Parent=MsG0080048669.01.T01
contig397end	CDS	29120	29235	29120	ID=MsG0080048669.01.T01:cds;Parent=MsG0080048669.01.T01
contig397end	three_prime_UTR	28903	29119	28903	ID=MsG0080048669.01.T01:three_prime_utr;Parent=MsG0080048669.01.T01

Gene Sequence

>MsG0080048669.01.T01

ATGCAGTTGATATTGAACGGCTCTAGCAGTGGAGTTCCTATGACAAATTCAGCATTTTTCGGAACATACCTTAAGAAAGCTAGTTCTGCGATTTCCAGCCAGAAGAGTTTGTCTGGTAACTTTAAGGTTTCGGCAAAAATTGACTATAATGAGGAAAAGCAGACCTCAAAGGACAGATGGGCTGGACTTGCATATGACACCTCAGATGACCAACAGGATATCACAAGGGGAAAGGGAAAGGTGGACAGTGTCTTTCAAGCTCCAACGGATGCTGGAACTCACTATGCTGTCATGAGTTCCTATGATTATATCAGTACCGGTCTTCGCCAGTACAATATGGACAACACAGTTGATGGCCTGTATATTGCACCCGCATTTATGGACAAGCTTGTCGTTCACATCACTAAGAACTTTCTCAACTTGCCAAATATTAAGATTCCTCTCATTTTGGGCATTTGGGGAGGCAAAGGTCAAGGAAAGTCATTTCAATGTGAGCTTGTCTTTGCCAAGATGGGAATCAGCCCTATAATGATGAGCGCCGGAGAATTGGAAAGTGGAAATGCAGGAGAGCCTGCAAAGTTGATTAGGCAAAGGTACCGTGAGGCGTCCGACATTATCAGAAAAGGAAAGATGTGCTGTCTCTTCATCAATGATCTTGATGCAGGAGCTGGTAGAATGGGTGGCACTACTCAATACACAGTCAATAACCAGATGGTAAATGCAACCCTCATGAACATTGCTGACAACCCTACCAATGTTCAACTTCCCGGTATGTACAACAAAGAAGAGAATGCCCGCGTCCCTATCATCGTCACTGGAAATGACTTCTCTACGTTGTATGCTCCTTTAATTCGTGACGGACGCATGGAAAAATTCTATTGGGCGCCTACTCGTGATGATAGAGTTGGTGTTTGTCAAGGTATTTTCAGGACAGATGGTGTCCCTAAGGATGACATTGTCAAGATTGTCGACACATTCCCTGGACAGTCAATCGATTTTTTTGGTGCCTTGAGGGCTAGAGTGTATGACGATGAAGTCAGGAATTGGATATCTGGTATTGGCGTGGAGAGTATTGGAAAAAGACTTGTGAATTCAAAAGAGGGACCTCCAACTTTCGAGCAACCAAAGATGACTCTTGACAAGCTACTAGAGTATGGAAATATGCTTGTCCAAGAACAAGAGAATGTGAAGAGGGTCCAATTAGCTGACAAATACCTCAGTGAGGCAGCTCTTGGAGATGCAAATAATGATGCTATCAAGACTGGAAACTTCTATGGACAAGGAGCTCAGCAAGTTCCTTTACCTGTCCCAGAAGGTTGCGCTGACCCTTCTGCAGAAAACTATGATCCGACAGCCAGAAGTGACGATGGAAGTTGCACATACACTTTTTAG

Protein sequence

>MsG0080048669.01.T01

MQLILNGSSSGVPMTNSAFFGTYLKKASSAISSQKSLSGNFKVSAKIDYNEEKQTSKDRWAGLAYDTSDDQQDITRGKGKVDSVFQAPTDAGTHYAVMSSYDYISTGLRQYNMDNTVDGLYIAPAFMDKLVVHITKNFLNLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGISPIMMSAGELESGNAGEPAKLIRQRYREASDIIRKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCQGIFRTDGVPKDDIVKIVDTFPGQSIDFFGALRARVYDDEVRNWISGIGVESIGKRLVNSKEGPPTFEQPKMTLDKLLEYGNMLVQEQENVKRVQLADKYLSEAALGDANNDAIKTGNFYGQGAQQVPLPVPEGCADPSAENYDPTARSDDGSCTYTF*