AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023217.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTTGTAATGAGCTTATT+TGG 0.218893 4:+82278206 MsG0480023217.01.T01:CDS
CAAGCTATGGCTGGAATTAA+TGG 0.225855 4:+82277978 MsG0480023217.01.T01:CDS
TGCTGCAAAAGCAGGGTTAC+AGG 0.278635 4:+82278061 MsG0480023217.01.T01:CDS
GGTTTGTAATGAGCTTATTT+GGG 0.370406 4:+82278207 MsG0480023217.01.T01:CDS
TTGAGGGAAATGAGGGAAGT+GGG 0.420587 4:+82278185 MsG0480023217.01.T01:CDS
TCTGTTGTGGCAATTCCCTT+TGG 0.428638 4:+82278152 MsG0480023217.01.T01:CDS
ATTTGTTGTGCTGCAAAAGC+AGG 0.431608 4:+82278053 MsG0480023217.01.T01:CDS
CTTTGGAAGAAGGTTCTTGA+GGG 0.435326 4:+82278169 MsG0480023217.01.T01:CDS
CATTAATTCCAGCCATAGCT+TGG 0.438157 4:-82277977 None:intergenic
CAAGGACTTTGAGTTGATCA+TGG 0.443749 4:+82278259 MsG0480023217.01.T01:CDS
TGGCAATTCCCTTTGGAAGA+AGG 0.444343 4:+82278159 MsG0480023217.01.T01:CDS
CTCAAGAACCTTCTTCCAAA+GGG 0.458123 4:-82278167 None:intergenic
TGCTAGTGCTGTTCCTGCTA+TGG 0.458852 4:+82278109 MsG0480023217.01.T01:CDS
TTATTTGGGTTGTGGAACTT+TGG 0.470489 4:+82278221 MsG0480023217.01.T01:CDS
TAATGAGCTTATTTGGGTTG+TGG 0.472958 4:+82278213 MsG0480023217.01.T01:CDS
ATTAATTCCAGCCATAGCTT+GGG 0.480687 4:-82277976 None:intergenic
TGGTGAACTAGTAGTGTTCA+AGG 0.485829 4:+82278241 MsG0480023217.01.T01:CDS
CCTCAAGAACCTTCTTCCAA+AGG 0.498567 4:-82278168 None:intergenic
AAGGTTCTTGAGGGAAATGA+GGG 0.499781 4:+82278178 MsG0480023217.01.T01:CDS
CATCTATATCAACTCATCAA+TGG 0.506082 4:-82278303 None:intergenic
GATCATGGTCATTGCAATGA+TGG 0.513443 4:+82278274 MsG0480023217.01.T01:CDS
CTTGAGGGAAATGAGGGAAG+TGG 0.514770 4:+82278184 MsG0480023217.01.T01:CDS
TACAGAACCCAAGCTATGGC+TGG 0.516463 4:+82277969 MsG0480023217.01.T01:CDS
TGAGGGAAATGAGGGAAGTG+GGG 0.522481 4:+82278186 MsG0480023217.01.T01:CDS
GAAGGTTCTTGAGGGAAATG+AGG 0.523923 4:+82278177 MsG0480023217.01.T01:CDS
CCTTTGGAAGAAGGTTCTTG+AGG 0.537577 4:+82278168 MsG0480023217.01.T01:CDS
AAACTATCAACTTCCATAGC+AGG 0.544376 4:-82278122 None:intergenic
ATAGCAGGAACAGCACTAGC+AGG 0.556768 4:-82278107 None:intergenic
GTTGTACAGAACCCAAGCTA+TGG 0.571265 4:+82277965 MsG0480023217.01.T01:CDS
TTTGTTGTGCTGCAAAAGCA+GGG 0.597469 4:+82278054 MsG0480023217.01.T01:CDS
CAAATAGCTGAAATAGCAGA+AGG 0.608735 4:-82278035 None:intergenic
GTAACAGAAGTGCTAAACCA+CGG 0.728265 4:+82278000 MsG0480023217.01.T01:CDS
GAAGGATCATCAGACAACCG+TGG 0.746201 4:-82278017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTTTAATGTTTTCTGTTG+TGG + Chr4:82278139-82278158 MsG0480023217.01.T01:CDS 25.0%
GGTTTGTAATGAGCTTATTT+GGG + Chr4:82278207-82278226 MsG0480023217.01.T01:CDS 30.0%
AAACTATCAACTTCCATAGC+AGG - Chr4:82278125-82278144 None:intergenic 35.0%
ATTAATTCCAGCCATAGCTT+GGG - Chr4:82277979-82277998 None:intergenic 35.0%
CAAATAGCTGAAATAGCAGA+AGG - Chr4:82278038-82278057 None:intergenic 35.0%
GGGTTTGTAATGAGCTTATT+TGG + Chr4:82278206-82278225 MsG0480023217.01.T01:CDS 35.0%
!! TAATGAGCTTATTTGGGTTG+TGG + Chr4:82278213-82278232 MsG0480023217.01.T01:CDS 35.0%
!! TTATTTGGGTTGTGGAACTT+TGG + Chr4:82278221-82278240 MsG0480023217.01.T01:CDS 35.0%
AAGGTTCTTGAGGGAAATGA+GGG + Chr4:82278178-82278197 MsG0480023217.01.T01:CDS 40.0%
CAAGCTATGGCTGGAATTAA+TGG + Chr4:82277978-82277997 MsG0480023217.01.T01:CDS 40.0%
CATTAATTCCAGCCATAGCT+TGG - Chr4:82277980-82277999 None:intergenic 40.0%
CTCAAGAACCTTCTTCCAAA+GGG - Chr4:82278170-82278189 None:intergenic 40.0%
GATCATGGTCATTGCAATGA+TGG + Chr4:82278274-82278293 MsG0480023217.01.T01:CDS 40.0%
TGGTGAACTAGTAGTGTTCA+AGG + Chr4:82278241-82278260 MsG0480023217.01.T01:CDS 40.0%
! CTTTGGAAGAAGGTTCTTGA+GGG + Chr4:82278169-82278188 MsG0480023217.01.T01:CDS 40.0%
! TTTGTTGTGCTGCAAAAGCA+GGG + Chr4:82278054-82278073 MsG0480023217.01.T01:CDS 40.0%
!! ATTTGTTGTGCTGCAAAAGC+AGG + Chr4:82278053-82278072 MsG0480023217.01.T01:CDS 40.0%
!! CAAGGACTTTGAGTTGATCA+TGG + Chr4:82278259-82278278 MsG0480023217.01.T01:CDS 40.0%
!! GTAACAGAAGTGCTAAACCA+CGG + Chr4:82278000-82278019 MsG0480023217.01.T01:CDS 40.0%
CCTCAAGAACCTTCTTCCAA+AGG - Chr4:82278171-82278190 None:intergenic 45.0%
GAAGGTTCTTGAGGGAAATG+AGG + Chr4:82278177-82278196 MsG0480023217.01.T01:CDS 45.0%
GTTGTACAGAACCCAAGCTA+TGG + Chr4:82277965-82277984 MsG0480023217.01.T01:CDS 45.0%
TCTGTTGTGGCAATTCCCTT+TGG + Chr4:82278152-82278171 MsG0480023217.01.T01:CDS 45.0%
TTGAGGGAAATGAGGGAAGT+GGG + Chr4:82278185-82278204 MsG0480023217.01.T01:CDS 45.0%
! CCTTTGGAAGAAGGTTCTTG+AGG + Chr4:82278168-82278187 MsG0480023217.01.T01:CDS 45.0%
! TGGCAATTCCCTTTGGAAGA+AGG + Chr4:82278159-82278178 MsG0480023217.01.T01:CDS 45.0%
ATAGCAGGAACAGCACTAGC+AGG - Chr4:82278110-82278129 None:intergenic 50.0%
CTTGAGGGAAATGAGGGAAG+TGG + Chr4:82278184-82278203 MsG0480023217.01.T01:CDS 50.0%
GAAGGATCATCAGACAACCG+TGG - Chr4:82278020-82278039 None:intergenic 50.0%
TACAGAACCCAAGCTATGGC+TGG + Chr4:82277969-82277988 MsG0480023217.01.T01:CDS 50.0%
TGAGGGAAATGAGGGAAGTG+GGG + Chr4:82278186-82278205 MsG0480023217.01.T01:CDS 50.0%
TGCTGCAAAAGCAGGGTTAC+AGG + Chr4:82278061-82278080 MsG0480023217.01.T01:CDS 50.0%
! TGCTAGTGCTGTTCCTGCTA+TGG + Chr4:82278109-82278128 MsG0480023217.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 82277963 82278322 82277963 ID=MsG0480023217.01;Name=MsG0480023217.01
Chr4 mRNA 82277963 82278322 82277963 ID=MsG0480023217.01.T01;Parent=MsG0480023217.01;Name=MsG0480023217.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|119
Chr4 exon 82277963 82278322 82277963 ID=MsG0480023217.01.T01:exon:2437;Parent=MsG0480023217.01.T01
Chr4 CDS 82277963 82278322 82277963 ID=MsG0480023217.01.T01:cds;Parent=MsG0480023217.01.T01
Gene Sequence

>MsG0480023217.01.T01

ATGTTGTACAGAACCCAAGCTATGGCTGGAATTAATGGTAACAGAAGTGCTAAACCACGGTTGTCTGATGATCCTTCTGCTATTTCAGCTATTTGTTGTGCTGCAAAAGCAGGGTTACAGGTTGAGAGTGTTGAGAATATTGCTCCTGCTAGTGCTGTTCCTGCTATGGAAGTTGATAGTTTAATGTTTTCTGTTGTGGCAATTCCCTTTGGAAGAAGGTTCTTGAGGGAAATGAGGGAAGTGGGGTTTGTAATGAGCTTATTTGGGTTGTGGAACTTTGGTGAACTAGTAGTGTTCAAGGACTTTGAGTTGATCATGGTCATTGCAATGATGGTTGATGCCATTGATGAGTTGATATAG

Protein sequence

>MsG0480023217.01.T01

MLYRTQAMAGINGNRSAKPRLSDDPSAISAICCAAKAGLQVESVENIAPASAVPAMEVDSLMFSVVAIPFGRRFLREMREVGFVMSLFGLWNFGELVVFKDFELIMVIAMMVDAIDELI*