AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039860.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039860.01.T01 MTR_7g080530 99.020 102 1 0 43 144 266 367 1.33e-67 209
MsG0780039860.01.T01 MTR_2g027270 70.707 99 29 0 44 142 157 255 1.13e-43 144
MsG0780039860.01.T01 MTR_2g027270 70.000 100 30 0 43 142 203 302 2.40e-43 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039860.01.T01 AT5G36790 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36700 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36700 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36700 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36790 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36790 87.255 102 13 0 43 144 261 362 1.16e-58 186
MsG0780039860.01.T01 AT5G36700 93.056 72 5 0 43 114 261 332 2.51e-41 140
MsG0780039860.01.T01 AT5G47760 63.918 97 35 0 43 139 201 297 4.74e-39 134

Find 42 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTATATTTATGTTAATT+TGG 0.122755 7:-69819544 None:intergenic
GTAATCAATAATGAATTATT+TGG 0.165938 7:+69819292 MsG0780039860.01.T01:three_prime_UTR
TCAATCTTGTTTGGTATGTT+AGG 0.269887 7:+69819496 MsG0780039860.01.T01:three_prime_UTR
TCATACAGCTGCAGCCTTTA+GGG 0.290646 7:-69819127 None:intergenic
CCAAATTAACATAAATATAA+AGG 0.313614 7:+69819544 MsG0780039860.01.T01:three_prime_UTR
ATTATTGATTACTTCATTGT+AGG 0.327277 7:-69819281 None:intergenic
ATCATACAGCTGCAGCCTTT+AGG 0.327656 7:-69819128 None:intergenic
AGGTTCAAGAATAGTGTAAA+TGG 0.357478 7:+69819564 MsG0780039860.01.T01:three_prime_UTR
AGTCACAGATATGTATGGTT+GGG 0.422366 7:+69818953 MsG0780039860.01.T01:CDS
ATCTCACAGACGCTCAAGAA+TGG 0.434908 7:+69818249 MsG0780039860.01.T01:CDS
TCTGGTTGTATGGAGTTGTC+TGG 0.445662 7:-69819072 None:intergenic
TCCTCTCAAAGTTGATTACA+AGG 0.447493 7:-69819225 None:intergenic
TTCAAAGTCACAGATATGTA+TGG 0.459793 7:+69818948 MsG0780039860.01.T01:CDS
CAACGCGAACCACTAGTTGT+TGG 0.464212 7:+69818743 MsG0780039860.01.T01:CDS
GGTATAGAAGTCTGGTTGTA+TGG 0.467427 7:-69819082 None:intergenic
TTCCTTTGAAATGACAGGTA+TGG 0.470466 7:+69818161 MsG0780039860.01.T01:CDS
TAGTCCATCATGAAAGTTGA+GGG 0.477251 7:-69818770 None:intergenic
CTCAGGTGTCACCACACTGC+CGG 0.477299 7:+69819038 MsG0780039860.01.T01:CDS
TCTCACAGACGCTCAAGAAT+GGG 0.490894 7:+69818250 MsG0780039860.01.T01:CDS
AAAACCCTCAACTTTCATGA+TGG 0.494302 7:+69818766 MsG0780039860.01.T01:CDS
AAAACTCTACTTGTCCTCTC+AGG 0.505930 7:+69819021 MsG0780039860.01.T01:CDS
TGCATCAAAGCAAGAGGAAG+TGG 0.506064 7:-69819463 None:intergenic
CATACCAAACAAGATTGAAA+AGG 0.508278 7:-69819491 None:intergenic
TTCCATACCTGTCATTTCAA+AGG 0.519148 7:-69818163 None:intergenic
GTAGCAATGAAAAGACATCC+AGG 0.552022 7:-69818206 None:intergenic
GTCACAGATATGTATGGTTG+GGG 0.555276 7:+69818954 MsG0780039860.01.T01:CDS
AAGTCACAGATATGTATGGT+TGG 0.555635 7:+69818952 MsG0780039860.01.T01:CDS
TCTATGGTTGGTGCTCTCGT+TGG 0.556722 7:+69818713 MsG0780039860.01.T01:CDS
CTGTTCTTAAATATTTGAGA+AGG 0.584086 7:-69819408 None:intergenic
TCCTTGTAATCAACTTTGAG+AGG 0.584935 7:+69819224 MsG0780039860.01.T01:three_prime_UTR
TAGCAATGAAAAGACATCCA+GGG 0.592708 7:-69818205 None:intergenic
ACAGACGCTCAAGAATGGGC+AGG 0.593223 7:+69818254 MsG0780039860.01.T01:CDS
AAGAACAGTCAATTTCTGCA+AGG 0.598818 7:+69819423 MsG0780039860.01.T01:three_prime_UTR
TTGTCTGGACTCTGTAGCAC+CGG 0.605890 7:-69819057 None:intergenic
TTAAATATTTGAGAAGGACA+TGG 0.613496 7:-69819402 None:intergenic
GTATACTGCATCAAAGCAAG+AGG 0.619062 7:-69819469 None:intergenic
GTAGTCCATCATGAAAGTTG+AGG 0.626905 7:-69818771 None:intergenic
CTGTGTATACGTGAAAACCC+TGG 0.633110 7:+69818188 MsG0780039860.01.T01:CDS
TCAATTTCTGCAAGGCGAGG+AGG 0.635663 7:+69819431 MsG0780039860.01.T01:three_prime_UTR
CAGTCAATTTCTGCAAGGCG+AGG 0.646009 7:+69819428 MsG0780039860.01.T01:three_prime_UTR
ACTCTGTAGCACCGGCAGTG+TGG 0.653090 7:-69819049 None:intergenic
GCAGTGTGGTGACACCTGAG+AGG 0.697232 7:-69819035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGTCATGAAATAAAAT+CGG - Chr7:69818309-69818328 None:intergenic 15.0%
!! CCAAATTAACATAAATATAA+AGG + Chr7:69819544-69819563 MsG0780039860.01.T01:three_prime_UTR 15.0%
!! CCTTTATATTTATGTTAATT+TGG - Chr7:69819547-69819566 None:intergenic 15.0%
!! GTAATCAATAATGAATTATT+TGG + Chr7:69819292-69819311 MsG0780039860.01.T01:three_prime_UTR 15.0%
!! TTTGATTAAGATGTAAAAAA+TGG + Chr7:69819356-69819375 MsG0780039860.01.T01:three_prime_UTR 15.0%
!!! AATTTTGAAAACTCTTTTTT+GGG - Chr7:69818482-69818501 None:intergenic 15.0%
!! TAATGTCATGAAATAAAATC+GGG - Chr7:69818308-69818327 None:intergenic 20.0%
!! TATATTTGTGTTTGAATTTC+AGG + Chr7:69818683-69818702 MsG0780039860.01.T01:intron 20.0%
!!! AAGAAAATCTGAAATTTTGT+TGG - Chr7:69819106-69819125 None:intergenic 20.0%
!!! ATTATTGATTACTTCATTGT+AGG - Chr7:69819284-69819303 None:intergenic 20.0%
!!! ATTTTGAAAACTCTTTTTTG+GGG - Chr7:69818481-69818500 None:intergenic 20.0%
!!! GAATTTTGAAAACTCTTTTT+TGG - Chr7:69818483-69818502 None:intergenic 20.0%
!!! TTGTAATTTTATTGAACAAC+AGG + Chr7:69818917-69818936 MsG0780039860.01.T01:intron 20.0%
! CTGTTCTTAAATATTTGAGA+AGG - Chr7:69819411-69819430 None:intergenic 25.0%
! TTAAATATTTGAGAAGGACA+TGG - Chr7:69819405-69819424 None:intergenic 25.0%
!! ATTTTATTGAACAACAGGTT+TGG + Chr7:69818922-69818941 MsG0780039860.01.T01:intron 25.0%
!! TATACCTTTTCAATCTTGTT+TGG + Chr7:69819487-69819506 MsG0780039860.01.T01:three_prime_UTR 25.0%
!!! GTTTGTGGTTAATTTGTTAA+GGG - Chr7:69818454-69818473 None:intergenic 25.0%
!!! TGTTTGTGGTTAATTTGTTA+AGG - Chr7:69818455-69818474 None:intergenic 25.0%
!!! TTTGTGGTTAATTTGTTAAG+GGG - Chr7:69818453-69818472 None:intergenic 25.0%
AAAATCGGGTAATAGTGAAA+GGG - Chr7:69818294-69818313 None:intergenic 30.0%
AGATGTAAAAAATGGTGAAC+AGG + Chr7:69819364-69819383 MsG0780039860.01.T01:three_prime_UTR 30.0%
AGGTTCAAGAATAGTGTAAA+TGG + Chr7:69819564-69819583 MsG0780039860.01.T01:three_prime_UTR 30.0%
ATTTGTGTTTGAATTTCAGG+TGG + Chr7:69818686-69818705 MsG0780039860.01.T01:intron 30.0%
CATACCAAACAAGATTGAAA+AGG - Chr7:69819494-69819513 None:intergenic 30.0%
TAAAATCGGGTAATAGTGAA+AGG - Chr7:69818295-69818314 None:intergenic 30.0%
TAAAATTACAACACAGTCAG+AGG - Chr7:69818908-69818927 None:intergenic 30.0%
TAGGTGGTATATTACAACTA+AGG + Chr7:69818555-69818574 MsG0780039860.01.T01:intron 30.0%
TCTGTAAACAAACTTGCAAA+TGG - Chr7:69818877-69818896 None:intergenic 30.0%
TTCAAAGTCACAGATATGTA+TGG + Chr7:69818948-69818967 MsG0780039860.01.T01:CDS 30.0%
! ATTTTGTTGGTATAGAAGTC+TGG - Chr7:69819093-69819112 None:intergenic 30.0%
! TCAATCTTGTTTGGTATGTT+AGG + Chr7:69819496-69819515 MsG0780039860.01.T01:three_prime_UTR 30.0%
! TTAGACACTGACATTTTGTT+TGG + Chr7:69818982-69819001 MsG0780039860.01.T01:CDS 30.0%
! TTCAGATTTTCTTTCCCTAA+AGG + Chr7:69819113-69819132 MsG0780039860.01.T01:CDS 30.0%
! TTTGTGTTTGAATTTCAGGT+GGG + Chr7:69818687-69818706 MsG0780039860.01.T01:intron 30.0%
!! ATTTTGTTTGGACAAAATGG+TGG + Chr7:69818994-69819013 MsG0780039860.01.T01:CDS 30.0%
!! GACATTTTGTTTGGACAAAA+TGG + Chr7:69818991-69819010 MsG0780039860.01.T01:CDS 30.0%
!!! ATGTTTTCCTTTGAAATGAC+AGG + Chr7:69818156-69818175 MsG0780039860.01.T01:CDS 30.0%
AAAACCCTCAACTTTCATGA+TGG + Chr7:69818766-69818785 MsG0780039860.01.T01:CDS 35.0%
AAGAACAGTCAATTTCTGCA+AGG + Chr7:69819423-69819442 MsG0780039860.01.T01:three_prime_UTR 35.0%
AAGTCACAGATATGTATGGT+TGG + Chr7:69818952-69818971 MsG0780039860.01.T01:CDS 35.0%
AATCAACACGATCCATTACA+CGG + Chr7:69818584-69818603 MsG0780039860.01.T01:intron 35.0%
AGTCACAGATATGTATGGTT+GGG + Chr7:69818953-69818972 MsG0780039860.01.T01:CDS 35.0%
CATAGAAAGACTAGCGAATT+AGG + Chr7:69818536-69818555 MsG0780039860.01.T01:intron 35.0%
CATTCTTAAAAGTTGTGCTC+TGG + Chr7:69818647-69818666 MsG0780039860.01.T01:intron 35.0%
TAAAAAATGGTGAACAGGAG+AGG + Chr7:69819369-69819388 MsG0780039860.01.T01:three_prime_UTR 35.0%
TAGCAATGAAAAGACATCCA+GGG - Chr7:69818208-69818227 None:intergenic 35.0%
TAGTCCATCATGAAAGTTGA+GGG - Chr7:69818773-69818792 None:intergenic 35.0%
TCCTTGTAATCAACTTTGAG+AGG + Chr7:69819224-69819243 MsG0780039860.01.T01:three_prime_UTR 35.0%
! AGTTTTCAAAATGTCCGTGA+AGG + Chr7:69818347-69818366 MsG0780039860.01.T01:intron 35.0%
! TCCTCTCAAAGTTGATTACA+AGG - Chr7:69819228-69819247 None:intergenic 35.0%
! TTCCATACCTGTCATTTCAA+AGG - Chr7:69818166-69818185 None:intergenic 35.0%
! TTGTGTTTGAATTTCAGGTG+GGG + Chr7:69818688-69818707 MsG0780039860.01.T01:intron 35.0%
!! GGTTAATTTGTTAAGGGGAA+GGG - Chr7:69818448-69818467 None:intergenic 35.0%
!! TGGTTAATTTGTTAAGGGGA+AGG - Chr7:69818449-69818468 None:intergenic 35.0%
!! TTCCTTTGAAATGACAGGTA+TGG + Chr7:69818161-69818180 MsG0780039860.01.T01:CDS 35.0%
!!! CTTTTTTGGGGAAATGTTTG+TGG - Chr7:69818469-69818488 None:intergenic 35.0%
AAAACTCTACTTGTCCTCTC+AGG + Chr7:69819021-69819040 MsG0780039860.01.T01:CDS 40.0%
AAGGGTAAATGTTAGTAGCG+GGG - Chr7:69818430-69818449 None:intergenic 40.0%
AGAAAGACTAGCGAATTAGG+TGG + Chr7:69818539-69818558 MsG0780039860.01.T01:intron 40.0%
ATCAACACGATCCATTACAC+GGG + Chr7:69818585-69818604 MsG0780039860.01.T01:intron 40.0%
GAAGGGTAAATGTTAGTAGC+GGG - Chr7:69818431-69818450 None:intergenic 40.0%
GGAAGGGTAAATGTTAGTAG+CGG - Chr7:69818432-69818451 None:intergenic 40.0%
GGTATAGAAGTCTGGTTGTA+TGG - Chr7:69819085-69819104 None:intergenic 40.0%
GTAGCAATGAAAAGACATCC+AGG - Chr7:69818209-69818228 None:intergenic 40.0%
GTAGTCCATCATGAAAGTTG+AGG - Chr7:69818774-69818793 None:intergenic 40.0%
GTATACTGCATCAAAGCAAG+AGG - Chr7:69819472-69819491 None:intergenic 40.0%
GTCACAGATATGTATGGTTG+GGG + Chr7:69818954-69818973 MsG0780039860.01.T01:CDS 40.0%
TAATAGTGAAAGGGAGCGAA+AGG - Chr7:69818285-69818304 None:intergenic 40.0%
! TGTGTTTGAATTTCAGGTGG+GGG + Chr7:69818689-69818708 MsG0780039860.01.T01:intron 40.0%
ATCATACAGCTGCAGCCTTT+AGG - Chr7:69819131-69819150 None:intergenic 45.0%
ATCTCACAGACGCTCAAGAA+TGG + Chr7:69818249-69818268 MsG0780039860.01.T01:CDS 45.0%
ATTGATCGTTGGACCTCCTT+CGG - Chr7:69818407-69818426 None:intergenic 45.0%
CTGTGTATACGTGAAAACCC+TGG + Chr7:69818188-69818207 MsG0780039860.01.T01:CDS 45.0%
TATCGTAATGCCCCGTGTAA+TGG - Chr7:69818599-69818618 None:intergenic 45.0%
TCAACACGATCCATTACACG+GGG + Chr7:69818586-69818605 MsG0780039860.01.T01:intron 45.0%
TCATACAGCTGCAGCCTTTA+GGG - Chr7:69819130-69819149 None:intergenic 45.0%
TCTCACAGACGCTCAAGAAT+GGG + Chr7:69818250-69818269 MsG0780039860.01.T01:CDS 45.0%
TCTGGTTGTATGGAGTTGTC+TGG - Chr7:69819075-69819094 None:intergenic 45.0%
TGCATCAAAGCAAGAGGAAG+TGG - Chr7:69819466-69819485 None:intergenic 45.0%
! GAGGGTTTTCCAACAACTAG+TGG - Chr7:69818755-69818774 None:intergenic 45.0%
CAACGCGAACCACTAGTTGT+TGG + Chr7:69818743-69818762 MsG0780039860.01.T01:CDS 50.0%
CAGTCAATTTCTGCAAGGCG+AGG + Chr7:69819428-69819447 MsG0780039860.01.T01:three_prime_UTR 50.0%
GTGTCTCAACCCATGCTACA+GGG - Chr7:69818385-69818404 None:intergenic 50.0%
TCAATTTCTGCAAGGCGAGG+AGG + Chr7:69819431-69819450 MsG0780039860.01.T01:three_prime_UTR 50.0%
TGTCTCAACCCATGCTACAG+GGG - Chr7:69818384-69818403 None:intergenic 50.0%
TTGTCTGGACTCTGTAGCAC+CGG - Chr7:69819060-69819079 None:intergenic 50.0%
! AATTTCAGGTGGGGGCTCTA+TGG + Chr7:69818697-69818716 MsG0780039860.01.T01:intron 50.0%
! TAGTAGCGGGGATTGATCGT+TGG - Chr7:69818418-69818437 None:intergenic 50.0%
! TCTATGGTTGGTGCTCTCGT+TGG + Chr7:69818713-69818732 MsG0780039860.01.T01:CDS 50.0%
AACCCATGCTACAGGGGACA+GGG - Chr7:69818378-69818397 None:intergenic 55.0%
ACAGACGCTCAAGAATGGGC+AGG + Chr7:69818254-69818273 MsG0780039860.01.T01:CDS 55.0%
ATCCCTGTCCCCTGTAGCAT+GGG + Chr7:69818373-69818392 MsG0780039860.01.T01:intron 55.0%
GGGACAGGGATACTCCTTCA+CGG - Chr7:69818364-69818383 None:intergenic 55.0%
GGTGTCTCAACCCATGCTAC+AGG - Chr7:69818386-69818405 None:intergenic 55.0%
TATCCCTGTCCCCTGTAGCA+TGG + Chr7:69818372-69818391 MsG0780039860.01.T01:intron 55.0%
! AGCATGGGTTGAGACACCGA+AGG + Chr7:69818388-69818407 MsG0780039860.01.T01:intron 55.0%
! ATGGGTTGAGACACCGAAGG+AGG + Chr7:69818391-69818410 MsG0780039860.01.T01:intron 55.0%
CAACCCATGCTACAGGGGAC+AGG - Chr7:69818379-69818398 None:intergenic 60.0%
GCAGTGTGGTGACACCTGAG+AGG - Chr7:69819038-69819057 None:intergenic 60.0%
TCAGGTGGGGGCTCTATGGT+TGG + Chr7:69818701-69818720 MsG0780039860.01.T01:intron 60.0%
! CTCAGGTGTCACCACACTGC+CGG + Chr7:69819038-69819057 MsG0780039860.01.T01:CDS 60.0%
!! ACTCTGTAGCACCGGCAGTG+TGG - Chr7:69819052-69819071 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 69818156 69819587 69818156 ID=MsG0780039860.01;Name=MsG0780039860.01
Chr7 mRNA 69818156 69819587 69818156 ID=MsG0780039860.01.T01;Parent=MsG0780039860.01;Name=MsG0780039860.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0.5|0.66|1|0.5|0.66|3|438|144
Chr7 exon 69818156 69818279 69818156 ID=MsG0780039860.01.T01:exon:12812;Parent=MsG0780039860.01.T01
Chr7 exon 69818705 69818804 69818705 ID=MsG0780039860.01.T01:exon:12813;Parent=MsG0780039860.01.T01
Chr7 exon 69818939 69819587 69818939 ID=MsG0780039860.01.T01:exon:12814;Parent=MsG0780039860.01.T01
Chr7 CDS 69818156 69818279 69818156 ID=MsG0780039860.01.T01:cds;Parent=MsG0780039860.01.T01
Chr7 CDS 69818705 69818804 69818705 ID=MsG0780039860.01.T01:cds;Parent=MsG0780039860.01.T01
Chr7 CDS 69818939 69819149 69818939 ID=MsG0780039860.01.T01:cds;Parent=MsG0780039860.01.T01
Chr7 three_prime_UTR 69819150 69819587 69819150 ID=MsG0780039860.01.T01:three_prime_utr;Parent=MsG0780039860.01.T01
Gene Sequence

>MsG0780039860.01.T01

ATGTTTTCCTTTGAAATGACAGGTATGGAACTCTGTGTATACGTGAAAACCCTGGATGTCTTTTCATTGCTACAAACAGAGATGCTGTTACTCATCTCACAGACGCTCAAGAATGGGCAGGTTTGTGGGGGCTCTATGGTTGGTGCTCTCGTTGGATCTACTCAACGCGAACCACTAGTTGTTGGAAAACCCTCAACTTTCATGATGGACTACTTAGCAAACGAGTTTGGCATTTCAAAGTCACAGATATGTATGGTTGGGGACAGATTAGACACTGACATTTTGTTTGGACAAAATGGTGGCTGCAAAACTCTACTTGTCCTCTCAGGTGTCACCACACTGCCGGTGCTACAGAGTCCAGACAACTCCATACAACCAGACTTCTATACCAACAAAATTTCAGATTTTCTTTCCCTAAAGGCTGCAGCTGTATGA

Protein sequence

>MsG0780039860.01.T01

MFSFEMTGMELCVYVKTLDVFSLLQTEMLLLISQTLKNGQVCGGSMVGALVGSTQREPLVVGKPSTFMMDYLANEFGISKSQICMVGDRLDTDILFGQNGGCKTLLVLSGVTTLPVLQSPDNSIQPDFYTNKISDFLSLKAAAV*