Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018190.01.T01 | XP_003604248.1 | 96.195 | 473 | 17 | 1 | 1 | 473 | 1 | 472 | 0 | 949 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0480018190.01 | -0.840580 | 7.984157e-58 | 1.142243e-54 |
| MsG0080047910.01 | MsG0480018190.01 | 0.869093 | 4.007014e-66 | 1.484884e-62 |
| MsG0080047945.01 | MsG0480018190.01 | 0.861206 | 1.207044e-63 | 3.388188e-60 |
| MsG0080047955.01 | MsG0480018190.01 | 0.867810 | 1.039986e-65 | 3.678573e-62 |
| MsG0080047956.01 | MsG0480018190.01 | 0.885855 | 5.671484e-72 | 3.956076e-68 |
| MsG0080047967.01 | MsG0480018190.01 | 0.859193 | 4.899031e-63 | 1.282984e-59 |
| MsG0080048036.01 | MsG0480018190.01 | -0.816804 | 4.578481e-52 | 3.276822e-49 |
| MsG0080048059.01 | MsG0480018190.01 | 0.951650 | 1.303425e-109 | 3.026313e-104 |
| MsG0080048232.01 | MsG0480018190.01 | 0.802142 | 6.456673e-49 | 3.138946e-46 |
| MsG0080048256.01 | MsG0480018190.01 | -0.822335 | 2.502405e-53 | 2.088489e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018190.01.T01 | MTR_4g007080 | 96.195 | 473 | 17 | 1 | 1 | 473 | 1 | 472 | 0.0 | 949 |
Find 124 sgRNAs with CRISPR-Local
Find 220 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGCCACAATGCAGTCTA+GGG | 0.479022 | 4:+1430246 | MsG0480018190.01.T01:CDS |
| AAATGGAAGACTGGCTAGGA+AGG | 0.564182 | 4:+1430077 | MsG0480018190.01.T01:CDS |
| AAATTTCAGCCCACACTTCA+AGG | 0.435721 | 4:-1430291 | None:intergenic |
| AACGAACCATATTCGATATT+CGG | 0.330076 | 4:-1429027 | None:intergenic |
| AAGGAAGTTGAAGGAAGTTG+TGG | 0.489133 | 4:+1430153 | MsG0480018190.01.T01:CDS |
| AAGGCCACAATGCAGTCTAG+GGG | 0.695170 | 4:+1430247 | MsG0480018190.01.T01:CDS |
| AATACACAAAAGCAGGTGTA+AGG | 0.488855 | 4:+1430182 | MsG0480018190.01.T01:CDS |
| AATCCAAACAACAATCCCTA+TGG | 0.508106 | 4:+1429093 | MsG0480018190.01.T01:CDS |
| AATCCATAGGGATTGTTGTT+TGG | 0.334719 | 4:-1429096 | None:intergenic |
| AATGTGAATGCTGCAGGAGC+AGG | 0.472215 | 4:+1429778 | MsG0480018190.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACACTAGTTATGCTCAAA+AGG | - | Chr4:1431192-1431211 | None:intergenic | 30.0% | |
| AAATAGAGCGCAAGTAAAAA+AGG | + | Chr4:1430493-1430512 | MsG0480018190.01.T01:intron | 30.0% | |
| AACGAACCATATTCGATATT+CGG | - | Chr4:1429030-1429049 | None:intergenic | 30.0% | |
| AAGAGAGACTCTATATTAGA+GGG | - | Chr4:1430343-1430362 | None:intergenic | 30.0% | |
| ACAAATTAATGTTTCCTTCG+AGG | + | Chr4:1431824-1431843 | MsG0480018190.01.T01:three_prime_UTR | 30.0% | |
| ACAATTTCATCGACCAAAAA+TGG | + | Chr4:1431217-1431236 | MsG0480018190.01.T01:intron | 30.0% | |
| ATATTCATTCTGTCATTGTC+TGG | + | Chr4:1430569-1430588 | MsG0480018190.01.T01:intron | 30.0% | |
| ATGCAAGTCTAAATACTATC+AGG | - | Chr4:1429985-1430004 | None:intergenic | 30.0% | |
| CGCTTAAAATATCACTTTCA+AGG | + | Chr4:1430134-1430153 | MsG0480018190.01.T01:CDS | 30.0% | |
| CTACAAAGTTAGACATGTTA+AGG | - | Chr4:1428728-1428747 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 1428707 | 1431876 | 1428707 | ID=MsG0480018190.01;Name=MsG0480018190.01 |
| Chr4 | mRNA | 1428707 | 1431876 | 1428707 | ID=MsG0480018190.01.T01;Parent=MsG0480018190.01;Name=MsG0480018190.01.T01;_AED=0.25;_eAED=0.25;_QI=95|1|1|1|1|1|6|148|473 |
| Chr4 | exon | 1428707 | 1429416 | 1428707 | ID=MsG0480018190.01.T01:exon:508;Parent=MsG0480018190.01.T01 |
| Chr4 | exon | 1429673 | 1429863 | 1429673 | ID=MsG0480018190.01.T01:exon:509;Parent=MsG0480018190.01.T01 |
| Chr4 | exon | 1430069 | 1430326 | 1430069 | ID=MsG0480018190.01.T01:exon:510;Parent=MsG0480018190.01.T01 |
| Chr4 | exon | 1430639 | 1430744 | 1430639 | ID=MsG0480018190.01.T01:exon:511;Parent=MsG0480018190.01.T01 |
| Chr4 | exon | 1431369 | 1431454 | 1431369 | ID=MsG0480018190.01.T01:exon:512;Parent=MsG0480018190.01.T01 |
| Chr4 | exon | 1431563 | 1431876 | 1431563 | ID=MsG0480018190.01.T01:exon:513;Parent=MsG0480018190.01.T01 |
| Chr4 | five_prime_UTR | 1428707 | 1428801 | 1428707 | ID=MsG0480018190.01.T01:five_prime_utr;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1428802 | 1429416 | 1428802 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1429673 | 1429863 | 1429673 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1430069 | 1430326 | 1430069 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1430639 | 1430744 | 1430639 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1431369 | 1431454 | 1431369 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | CDS | 1431563 | 1431728 | 1431563 | ID=MsG0480018190.01.T01:cds;Parent=MsG0480018190.01.T01 |
| Chr4 | three_prime_UTR | 1431729 | 1431876 | 1431729 | ID=MsG0480018190.01.T01:three_prime_utr;Parent=MsG0480018190.01.T01 |
| Gene Sequence |
>MsG0480018190.01.T01 ATGGCTGCAACTGCAACATATAATTTACATACCAAAATTTCAAAACCATTTTCTCATTTCACTACAAACACATTTTCCATTCCTTCAACTTATCCAACCAATGTTTCATTGTTAACTTCATTAGACCATCCATCACATTGTTACAATTCAACTCGAAGACTAAATCTTCGCGTAAATGCCAAAAAGAACAATCCATGGCTAGACCCTTTTGACGATGGAGAAGACCCGAATATCGAATATGGTTCGTTGTTTTCAGACGGTAAACAAGACGAGGATCCAAGGCCACCGGATAATCCAAACAACAATCCCTATGGATTCTTGAAGTTTCCAGCTGGTTATATGGTTGAGATAGCTCCTTTAGGATTGAAAGTTAGAGGTGATGTTAGAAGATGTTGTTGTGTGGTATCAGGTGGTGTTTATGAGAATTTGTTGTTTTTTCCAATGATTCAGTTGATTAAGGATAGGTACCCTGGTGTTCAGATTGATGTTGTGGGTTCTGAGAGAGGGAAACAGTGTTATGAGTTGAATAAGAATGTTAGATGGGCTAATGTTTTTGATCCGGACGATGAATTTCCTGAGCCTGCTGAGTATACTGATTTTGTTGGTCAGCTAAAGAGTAGGTACTATGACATGATCTTGAGCACAAAATTGGCAGGAGTTGGCCATGCAGCATATTTATTCATGACAACCGCGCGAGATAGAGTTAGTTATGTCTACCCAAATGTGAATGCTGCAGGAGCAGGATTGTTTTTATCTGAAACATTCACACCAGAAAGTACAAATCTTTCGGAGGGAGGATATCATATGTATCATCAAATGGAAGACTGGCTAGGAAGGCCGTTTCGAAACGTTCCACGGCAGGTTGTGCCACCGCTTAAAATATCACTTTCAAGGAAGTTGAAGGAAGTTGTGGAGGAGAAATACACAAAAGCAGGTGTAAGGAAAGGAAGATATGTTGTGATTCATGGGATACAATCAGACTCAAAGGCCACAATGCAGTCTAGGGGTGACCCTGATAGCTTGCTACCCCTTGAAGTGTGGGCTGAAATTTCTGATGCTGTAAGGGAATTTACACCACTTTTTGTCATTCCACATGAGAAAGAAAGGGAAAATGTTGAGGAAATTGTTGGAGAAGATTCCTCCATAATCTTCATCACCACCCCTGGACAGCTGGCTGCTCTTATTAATGACTCAGCTGGAGTTATAGCAACCAATACCGCTGCAATTCAGCTCGCAAATGCGCGCGAAAAACCTAGTGTTGCACTATTTTGCTCTAAAGAGAAGGGAAACAAGTTTGTTCCGCAGGCAGAAGAGAAGAAATGCATTGTGATATCATCCAAGACGGGGAAGTTGATCGATATAGATGTTGAGGCTGTAAAAAGTGCAATTCAAACTTTCAATGTTTCTCTAGCTTTTGCATAG |
| Protein sequence |
>MsG0480018190.01.T01 MAATATYNLHTKISKPFSHFTTNTFSIPSTYPTNVSLLTSLDHPSHCYNSTRRLNLRVNAKKNNPWLDPFDDGEDPNIEYGSLFSDGKQDEDPRPPDNPNNNPYGFLKFPAGYMVEIAPLGLKVRGDVRRCCCVVSGGVYENLLFFPMIQLIKDRYPGVQIDVVGSERGKQCYELNKNVRWANVFDPDDEFPEPAEYTDFVGQLKSRYYDMILSTKLAGVGHAAYLFMTTARDRVSYVYPNVNAAGAGLFLSETFTPESTNLSEGGYHMYHQMEDWLGRPFRNVPRQVVPPLKISLSRKLKEVVEEKYTKAGVRKGRYVVIHGIQSDSKATMQSRGDPDSLLPLEVWAEISDAVREFTPLFVIPHEKERENVEEIVGEDSSIIFITTPGQLAALINDSAGVIATNTAAIQLANAREKPSVALFCSKEKGNKFVPQAEEKKCIVISSKTGKLIDIDVEAVKSAIQTFNVSLAFA* |
