Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024128.01.T01 | XP_003610706.1 | 97.143 | 210 | 5 | 1 | 1 | 210 | 1 | 209 | 1.04E-145 | 414 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0580024128.01 | 0.888061 | 8.241306e-73 | 6.286020e-69 |
| MsG0080047945.01 | MsG0580024128.01 | 0.889521 | 2.248259e-73 | 1.820078e-69 |
| MsG0080047955.01 | MsG0580024128.01 | 0.908677 | 1.316494e-81 | 2.470774e-77 |
| MsG0080047956.01 | MsG0580024128.01 | 0.916692 | 1.311053e-85 | 3.646253e-81 |
| MsG0080047967.01 | MsG0580024128.01 | 0.807265 | 5.499200e-50 | 3.049294e-47 |
| MsG0080047989.01 | MsG0580024128.01 | 0.803091 | 4.112156e-49 | 2.048012e-46 |
| MsG0080048059.01 | MsG0580024128.01 | 0.915963 | 3.149451e-85 | 8.458768e-81 |
| MsG0080048068.01 | MsG0580024128.01 | 0.824149 | 9.436774e-54 | 8.290359e-51 |
| MsG0080048528.01 | MsG0580024128.01 | 0.871278 | 7.719356e-67 | 3.095791e-63 |
| MsG0080048611.01 | MsG0580024128.01 | 0.885530 | 7.507718e-72 | 5.172174e-68 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024128.01.T01 | MTR_5g006130 | 97.143 | 210 | 5 | 1 | 1 | 210 | 1 | 209 | 1.26e-149 | 414 |
| MsG0580024128.01.T01 | MTR_1g104630 | 67.281 | 217 | 40 | 3 | 1 | 210 | 1 | 193 | 7.55e-95 | 275 |
Find 61 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAACAGTGTTTGGCTTT+AGG | 0.250321 | 5:+1195674 | MsG0580024128.01.T01:CDS |
| AAAGTTGATGGTTGTTTCCA+TGG | 0.433796 | 5:-1195329 | None:intergenic |
| AAATATTGAACCTCACCATT+AGG | 0.330180 | 5:-1195749 | None:intergenic |
| AACACACCTTCACCAATCTT+TGG | 0.314938 | 5:+1195503 | MsG0580024128.01.T01:CDS |
| AACCCTGGTCGTGAAGGAGT+TGG | 0.600436 | 5:+1195806 | MsG0580024128.01.T01:CDS |
| AAGAATTCTATGTGATCACA+TGG | 0.598132 | 5:+1195564 | MsG0580024128.01.T01:CDS |
| AAGATTGGTGAAGGTGTGTT+TGG | 0.388416 | 5:-1195500 | None:intergenic |
| AAGGAGTTGGTCAGAATTTC+AGG | 0.217942 | 5:+1195819 | MsG0580024128.01.T01:CDS |
| AAGGTTAACCCTGGTCGTGA+AGG | 0.571068 | 5:+1195800 | MsG0580024128.01.T01:CDS |
| AATATTGAACCTCACCATTA+GGG | 0.389009 | 5:-1195748 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATATTGAACCTCACCATT+AGG | - | Chr5:1195752-1195771 | None:intergenic | 30.0% | |
| AAGAATTCTATGTGATCACA+TGG | + | Chr5:1195564-1195583 | MsG0580024128.01.T01:CDS | 30.0% | |
| AATATTGAACCTCACCATTA+GGG | - | Chr5:1195751-1195770 | None:intergenic | 30.0% | |
| AGAATTCTATGTGATCACAT+GGG | + | Chr5:1195565-1195584 | MsG0580024128.01.T01:CDS | 30.0% | |
| CCAATTGAAGTTAAGTTTAC+TGG | + | Chr5:1195863-1195882 | MsG0580024128.01.T01:CDS | 30.0% | |
| CCAGTAAACTTAACTTCAAT+TGG | - | Chr5:1195866-1195885 | None:intergenic | 30.0% | |
| CAATATTTGCATCCTAAGGA+TGG | + | Chr5:1195764-1195783 | MsG0580024128.01.T01:CDS | 35.0% | |
| CTAACCTTCTTAAGTTAGCA+AGG | + | Chr5:1195651-1195670 | MsG0580024128.01.T01:CDS | 35.0% | |
| CTTGCTAACTTAAGAAGGTT+AGG | - | Chr5:1195653-1195672 | None:intergenic | 35.0% | |
| CTTTCCTTGCTAACTTAAGA+AGG | - | Chr5:1195658-1195677 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1195275 | 1195907 | 1195275 | ID=MsG0580024128.01;Name=MsG0580024128.01 |
| Chr5 | mRNA | 1195275 | 1195907 | 1195275 | ID=MsG0580024128.01.T01;Parent=MsG0580024128.01;Name=MsG0580024128.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|210 |
| Chr5 | exon | 1195275 | 1195907 | 1195275 | ID=MsG0580024128.01.T01:exon:23307;Parent=MsG0580024128.01.T01 |
| Chr5 | CDS | 1195275 | 1195907 | 1195275 | ID=MsG0580024128.01.T01:cds;Parent=MsG0580024128.01.T01 |
| Gene Sequence |
>MsG0580024128.01.T01 ATGGCAATGGCAACTCAAGCCTCTCTCTTCACTCCACCTCTCTCAAGCCCTAAACCATGGAAACAACCATCAACTTTATCCTTCATCTCTCTCAAACCCATCAAGTTCACAACAAGAACAACAAAAATCTCAGCTGCAGATGACAAAACAGAAGCAGCAGCACCAGTTGCAACAAAAGAAGAAGCTCCTGCTGCACCAGTTGGTTTCACTCCACCTGAACTAGACCCAAACACACCTTCACCAATCTTTGGAGGAAGCACTGGTGGTTTGTTACGTAAGGCACAAGTTGAAGAATTCTATGTGATCACATGGGAGTCACCTAAGGAACAAATCTTTGAGATGCCAACTGGTGGTGCAGCCATAATGAGAGAAGGTCCTAACCTTCTTAAGTTAGCAAGGAAAGAACAGTGTTTGGCTTTAGGAAATAGATTAAGGTCTAAGTACAAGATTAAGTATCAGTTTTACAGGGTTTTCCCTAATGGTGAGGTTCAATATTTGCATCCTAAGGATGGTGTGTACCCTGAGAAGGTTAACCCTGGTCGTGAAGGAGTTGGTCAGAATTTCAGGTCTATTGGAAAGAATGTTAGCCCAATTGAAGTTAAGTTTACTGGTAAGCAACCTTTTGATGTGTAA |
| Protein sequence |
>MsG0580024128.01.T01 MAMATQASLFTPPLSSPKPWKQPSTLSFISLKPIKFTTRTTKISAADDKTEAAAPVATKEEAPAAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGNRLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGREGVGQNFRSIGKNVSPIEVKFTGKQPFDV* |
