AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024128.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024128.01.T01 MTR_5g006130 97.143 210 5 1 1 210 1 209 1.26e-149 414
MsG0580024128.01.T01 MTR_1g104630 67.281 217 40 3 1 210 1 193 7.55e-95 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024128.01.T01 AT1G03130 73.684 209 49 3 3 210 1 204 2.55e-105 302
MsG0580024128.01.T01 AT4G02770 72.770 213 48 4 3 210 1 208 7.71e-102 293

Find 61 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGAAGTTAAGTTTAC+TGG 0.207430 5:+1195863 MsG0580024128.01.T01:CDS
AAGGAGTTGGTCAGAATTTC+AGG 0.217942 5:+1195819 MsG0580024128.01.T01:CDS
AAAGAACAGTGTTTGGCTTT+AGG 0.250321 5:+1195674 MsG0580024128.01.T01:CDS
GATGGTTGTTTCCATGGTTT+AGG 0.251347 5:-1195323 None:intergenic
CCAGTAAACTTAACTTCAAT+TGG 0.254455 5:-1195863 None:intergenic
TGGCTTTAGGAAATAGATTA+AGG 0.256198 5:+1195687 MsG0580024128.01.T01:CDS
AACACACCTTCACCAATCTT+TGG 0.314938 5:+1195503 MsG0580024128.01.T01:CDS
GTTAGGACCTTCTCTCATTA+TGG 0.318870 5:-1195633 None:intergenic
CGACCAGGGTTAACCTTCTC+AGG 0.322720 5:-1195794 None:intergenic
AAATATTGAACCTCACCATT+AGG 0.330180 5:-1195749 None:intergenic
ATGGTTGTTTCCATGGTTTA+GGG 0.335226 5:-1195322 None:intergenic
GGTCAGAATTTCAGGTCTAT+TGG 0.346075 5:+1195827 MsG0580024128.01.T01:CDS
CTTGCTAACTTAAGAAGGTT+AGG 0.358826 5:-1195650 None:intergenic
TGTTCTTGTTGTGAACTTGA+TGG 0.369555 5:-1195372 None:intergenic
AAGATTGGTGAAGGTGTGTT+TGG 0.388416 5:-1195500 None:intergenic
AATATTGAACCTCACCATTA+GGG 0.389009 5:-1195748 None:intergenic
AGCAAGGAAAGAACAGTGTT+TGG 0.399243 5:+1195667 MsG0580024128.01.T01:CDS
GAGATGAAGGATAAAGTTGA+TGG 0.402382 5:-1195341 None:intergenic
CTTTCCTTGCTAACTTAAGA+AGG 0.421239 5:-1195655 None:intergenic
GGTGTGTTTGGGTCTAGTTC+AGG 0.421350 5:-1195488 None:intergenic
CAGTAAACTTAACTTCAATT+GGG 0.432988 5:-1195862 None:intergenic
AAAGTTGATGGTTGTTTCCA+TGG 0.433796 5:-1195329 None:intergenic
GTTCTTGTTGTGAACTTGAT+GGG 0.443234 5:-1195371 None:intergenic
ATCTCAAAGATTTGTTCCTT+AGG 0.450732 5:-1195593 None:intergenic
GATGGGTTTGAGAGAGATGA+AGG 0.471436 5:-1195354 None:intergenic
TCAGGGTACACACCATCCTT+AGG 0.473133 5:-1195776 None:intergenic
CAAATCTTTGAGATGCCAAC+TGG 0.474824 5:+1195602 MsG0580024128.01.T01:CDS
GTGAAACCAACTGGTGCAGC+AGG 0.478227 5:-1195461 None:intergenic
AGATTGGTGAAGGTGTGTTT+GGG 0.480790 5:-1195499 None:intergenic
GTCAACCCTTACACATCAAA+AGG 0.481319 5:-1195893 None:intergenic
CCAATCTTTGGAGGAAGCAC+TGG 0.483310 5:+1195515 MsG0580024128.01.T01:CDS
CACTGGTGGTTTGTTACGTA+AGG 0.494140 5:+1195532 MsG0580024128.01.T01:CDS
CCAGTGCTTCCTCCAAAGAT+TGG 0.498438 5:-1195515 None:intergenic
TCAGGTGGAGTGAAACCAAC+TGG 0.512231 5:-1195470 None:intergenic
TGACCAACTCCTTCACGACC+AGG 0.519461 5:-1195809 None:intergenic
GGTTCAATATTTGCATCCTA+AGG 0.527977 5:+1195760 MsG0580024128.01.T01:CDS
CTAACCTTCTTAAGTTAGCA+AGG 0.531725 5:+1195651 MsG0580024128.01.T01:CDS
GAGAGGTGGAGTGAAGAGAG+AGG 0.532656 5:-1195294 None:intergenic
GGCTTGAGTTGCCATTGCCA+TGG 0.535458 5:-1195273 None:intergenic
TTATTCTTCAACCATGGCAA+TGG 0.536172 5:+1195262 None:intergenic
ACACCTTCACCAATCTTTGG+AGG 0.538603 5:+1195506 MsG0580024128.01.T01:CDS
GAAGCTCCTGCTGCACCAGT+TGG 0.541120 5:+1195455 MsG0580024128.01.T01:CDS
ATCTTTGGAGGAAGCACTGG+TGG 0.547257 5:+1195518 MsG0580024128.01.T01:CDS
GTGTTTGGGTCTAGTTCAGG+TGG 0.547713 5:-1195485 None:intergenic
CAATATTTGCATCCTAAGGA+TGG 0.551649 5:+1195764 MsG0580024128.01.T01:CDS
GGTTTAGGGCTTGAGAGAGG+TGG 0.569137 5:-1195308 None:intergenic
AAGGTTAACCCTGGTCGTGA+AGG 0.571068 5:+1195800 MsG0580024128.01.T01:CDS
CATGGTTTAGGGCTTGAGAG+AGG 0.581991 5:-1195311 None:intergenic
CTCTCTCAAGCCCTAAACCA+TGG 0.587901 5:+1195312 MsG0580024128.01.T01:CDS
GATCACATGGGAGTCACCTA+AGG 0.589857 5:+1195577 MsG0580024128.01.T01:CDS
GGATGGTGTGTACCCTGAGA+AGG 0.597067 5:+1195781 MsG0580024128.01.T01:CDS
AAGAATTCTATGTGATCACA+TGG 0.598132 5:+1195564 MsG0580024128.01.T01:CDS
AACCCTGGTCGTGAAGGAGT+TGG 0.600436 5:+1195806 MsG0580024128.01.T01:CDS
CTTCCTCCAAAGATTGGTGA+AGG 0.600903 5:-1195509 None:intergenic
ATTATGGCTGCACCACCAGT+TGG 0.609110 5:-1195617 None:intergenic
GACCAACTCCTTCACGACCA+GGG 0.644619 5:-1195808 None:intergenic
GGTGCAGCCATAATGAGAGA+AGG 0.648728 5:+1195626 MsG0580024128.01.T01:CDS
TACCCTGAGAAGGTTAACCC+TGG 0.651425 5:+1195791 MsG0580024128.01.T01:CDS
GACCAGGGTTAACCTTCTCA+GGG 0.658939 5:-1195793 None:intergenic
ATCTTTGAGATGCCAACTGG+TGG 0.674480 5:+1195605 MsG0580024128.01.T01:CDS
AGAATTCTATGTGATCACAT+GGG 0.727784 5:+1195565 MsG0580024128.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAGTAAACTTAACTTCAATT+GGG - Chr5:1195865-1195884 None:intergenic 25.0%
!! AGATTAAGTATCAGTTTTAC+AGG + Chr5:1195720-1195739 MsG0580024128.01.T01:CDS 25.0%
!! GATTAAGTATCAGTTTTACA+GGG + Chr5:1195721-1195740 MsG0580024128.01.T01:CDS 25.0%
AAATATTGAACCTCACCATT+AGG - Chr5:1195752-1195771 None:intergenic 30.0%
AAGAATTCTATGTGATCACA+TGG + Chr5:1195564-1195583 MsG0580024128.01.T01:CDS 30.0%
AATATTGAACCTCACCATTA+GGG - Chr5:1195751-1195770 None:intergenic 30.0%
AGAATTCTATGTGATCACAT+GGG + Chr5:1195565-1195584 MsG0580024128.01.T01:CDS 30.0%
CCAATTGAAGTTAAGTTTAC+TGG + Chr5:1195863-1195882 MsG0580024128.01.T01:CDS 30.0%
CCAGTAAACTTAACTTCAAT+TGG - Chr5:1195866-1195885 None:intergenic 30.0%
! ATCTCAAAGATTTGTTCCTT+AGG - Chr5:1195596-1195615 None:intergenic 30.0%
!! TGGCTTTAGGAAATAGATTA+AGG + Chr5:1195687-1195706 MsG0580024128.01.T01:CDS 30.0%
CAATATTTGCATCCTAAGGA+TGG + Chr5:1195764-1195783 MsG0580024128.01.T01:CDS 35.0%
CTAACCTTCTTAAGTTAGCA+AGG + Chr5:1195651-1195670 MsG0580024128.01.T01:CDS 35.0%
CTTGCTAACTTAAGAAGGTT+AGG - Chr5:1195653-1195672 None:intergenic 35.0%
CTTTCCTTGCTAACTTAAGA+AGG - Chr5:1195658-1195677 None:intergenic 35.0%
GAGATGAAGGATAAAGTTGA+TGG - Chr5:1195344-1195363 None:intergenic 35.0%
GGTTCAATATTTGCATCCTA+AGG + Chr5:1195760-1195779 MsG0580024128.01.T01:CDS 35.0%
! ATGGTTGTTTCCATGGTTTA+GGG - Chr5:1195325-1195344 None:intergenic 35.0%
! GTTCTTGTTGTGAACTTGAT+GGG - Chr5:1195374-1195393 None:intergenic 35.0%
! TGTTCTTGTTGTGAACTTGA+TGG - Chr5:1195375-1195394 None:intergenic 35.0%
! TTTTACAGGGTTTTCCCTAA+TGG + Chr5:1195734-1195753 MsG0580024128.01.T01:CDS 35.0%
!! AAAGAACAGTGTTTGGCTTT+AGG + Chr5:1195674-1195693 MsG0580024128.01.T01:CDS 35.0%
!! AAAGTTGATGGTTGTTTCCA+TGG - Chr5:1195332-1195351 None:intergenic 35.0%
AACACACCTTCACCAATCTT+TGG + Chr5:1195503-1195522 MsG0580024128.01.T01:CDS 40.0%
AGCAAGGAAAGAACAGTGTT+TGG + Chr5:1195667-1195686 MsG0580024128.01.T01:CDS 40.0%
GTTAGGACCTTCTCTCATTA+TGG - Chr5:1195636-1195655 None:intergenic 40.0%
! AAGATTGGTGAAGGTGTGTT+TGG - Chr5:1195503-1195522 None:intergenic 40.0%
! AAGGAGTTGGTCAGAATTTC+AGG + Chr5:1195819-1195838 MsG0580024128.01.T01:CDS 40.0%
! AGATTGGTGAAGGTGTGTTT+GGG - Chr5:1195502-1195521 None:intergenic 40.0%
! GATGGTTGTTTCCATGGTTT+AGG - Chr5:1195326-1195345 None:intergenic 40.0%
! GCTTCTTCTTTTGTTGCAAC+TGG - Chr5:1195440-1195459 None:intergenic 40.0%
! GGTCAGAATTTCAGGTCTAT+TGG + Chr5:1195827-1195846 MsG0580024128.01.T01:CDS 40.0%
!! CAAATCTTTGAGATGCCAAC+TGG + Chr5:1195602-1195621 MsG0580024128.01.T01:CDS 40.0%
ACACCTTCACCAATCTTTGG+AGG + Chr5:1195506-1195525 MsG0580024128.01.T01:CDS 45.0%
CTTCCTCCAAAGATTGGTGA+AGG - Chr5:1195512-1195531 None:intergenic 45.0%
! GATGGGTTTGAGAGAGATGA+AGG - Chr5:1195357-1195376 None:intergenic 45.0%
!! ATCTTTGAGATGCCAACTGG+TGG + Chr5:1195605-1195624 MsG0580024128.01.T01:CDS 45.0%
!! CACTGGTGGTTTGTTACGTA+AGG + Chr5:1195532-1195551 MsG0580024128.01.T01:CDS 45.0%
AAGGTTAACCCTGGTCGTGA+AGG + Chr5:1195800-1195819 MsG0580024128.01.T01:CDS 50.0%
ATTATGGCTGCACCACCAGT+TGG - Chr5:1195620-1195639 None:intergenic 50.0%
CCAGTGCTTCCTCCAAAGAT+TGG - Chr5:1195518-1195537 None:intergenic 50.0%
CTCTCTCAAGCCCTAAACCA+TGG + Chr5:1195312-1195331 MsG0580024128.01.T01:CDS 50.0%
GACCAGGGTTAACCTTCTCA+GGG - Chr5:1195796-1195815 None:intergenic 50.0%
GATCACATGGGAGTCACCTA+AGG + Chr5:1195577-1195596 MsG0580024128.01.T01:CDS 50.0%
GGTGCAGCCATAATGAGAGA+AGG + Chr5:1195626-1195645 MsG0580024128.01.T01:CDS 50.0%
TACCCTGAGAAGGTTAACCC+TGG + Chr5:1195791-1195810 MsG0580024128.01.T01:CDS 50.0%
TCAGGGTACACACCATCCTT+AGG - Chr5:1195779-1195798 None:intergenic 50.0%
TCAGGTGGAGTGAAACCAAC+TGG - Chr5:1195473-1195492 None:intergenic 50.0%
! CAGGGTTTTCCCTAATGGTG+AGG + Chr5:1195739-1195758 MsG0580024128.01.T01:CDS 50.0%
! CATGGTTTAGGGCTTGAGAG+AGG - Chr5:1195314-1195333 None:intergenic 50.0%
! CCAATCTTTGGAGGAAGCAC+TGG + Chr5:1195515-1195534 MsG0580024128.01.T01:CDS 50.0%
! GGTGTGTTTGGGTCTAGTTC+AGG - Chr5:1195491-1195510 None:intergenic 50.0%
! GTGTTTGGGTCTAGTTCAGG+TGG - Chr5:1195488-1195507 None:intergenic 50.0%
!! ATCTTTGGAGGAAGCACTGG+TGG + Chr5:1195518-1195537 MsG0580024128.01.T01:CDS 50.0%
AACCCTGGTCGTGAAGGAGT+TGG + Chr5:1195806-1195825 MsG0580024128.01.T01:CDS 55.0%
CGACCAGGGTTAACCTTCTC+AGG - Chr5:1195797-1195816 None:intergenic 55.0%
GACCAACTCCTTCACGACCA+GGG - Chr5:1195811-1195830 None:intergenic 55.0%
GAGAGGTGGAGTGAAGAGAG+AGG - Chr5:1195297-1195316 None:intergenic 55.0%
GGATGGTGTGTACCCTGAGA+AGG + Chr5:1195781-1195800 MsG0580024128.01.T01:CDS 55.0%
GTGAAACCAACTGGTGCAGC+AGG - Chr5:1195464-1195483 None:intergenic 55.0%
TGACCAACTCCTTCACGACC+AGG - Chr5:1195812-1195831 None:intergenic 55.0%
! GGTTTAGGGCTTGAGAGAGG+TGG - Chr5:1195311-1195330 None:intergenic 55.0%
GAAGCTCCTGCTGCACCAGT+TGG + Chr5:1195455-1195474 MsG0580024128.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 1195275 1195907 1195275 ID=MsG0580024128.01;Name=MsG0580024128.01
Chr5 mRNA 1195275 1195907 1195275 ID=MsG0580024128.01.T01;Parent=MsG0580024128.01;Name=MsG0580024128.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|210
Chr5 exon 1195275 1195907 1195275 ID=MsG0580024128.01.T01:exon:23307;Parent=MsG0580024128.01.T01
Chr5 CDS 1195275 1195907 1195275 ID=MsG0580024128.01.T01:cds;Parent=MsG0580024128.01.T01
Gene Sequence

>MsG0580024128.01.T01

ATGGCAATGGCAACTCAAGCCTCTCTCTTCACTCCACCTCTCTCAAGCCCTAAACCATGGAAACAACCATCAACTTTATCCTTCATCTCTCTCAAACCCATCAAGTTCACAACAAGAACAACAAAAATCTCAGCTGCAGATGACAAAACAGAAGCAGCAGCACCAGTTGCAACAAAAGAAGAAGCTCCTGCTGCACCAGTTGGTTTCACTCCACCTGAACTAGACCCAAACACACCTTCACCAATCTTTGGAGGAAGCACTGGTGGTTTGTTACGTAAGGCACAAGTTGAAGAATTCTATGTGATCACATGGGAGTCACCTAAGGAACAAATCTTTGAGATGCCAACTGGTGGTGCAGCCATAATGAGAGAAGGTCCTAACCTTCTTAAGTTAGCAAGGAAAGAACAGTGTTTGGCTTTAGGAAATAGATTAAGGTCTAAGTACAAGATTAAGTATCAGTTTTACAGGGTTTTCCCTAATGGTGAGGTTCAATATTTGCATCCTAAGGATGGTGTGTACCCTGAGAAGGTTAACCCTGGTCGTGAAGGAGTTGGTCAGAATTTCAGGTCTATTGGAAAGAATGTTAGCCCAATTGAAGTTAAGTTTACTGGTAAGCAACCTTTTGATGTGTAA

Protein sequence

>MsG0580024128.01.T01

MAMATQASLFTPPLSSPKPWKQPSTLSFISLKPIKFTTRTTKISAADDKTEAAAPVATKEEAPAAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGNRLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGREGVGQNFRSIGKNVSPIEVKFTGKQPFDV*