AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041809.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041809.01.T01 MTR_7g118290 89.809 157 9 3 15 170 15 165 1.11e-97 280
MsG0780041809.01.T01 MTR_7g118290 76.433 157 9 4 15 170 15 144 6.73e-76 224
MsG0780041809.01.T01 MTR_7g118290 85.827 127 10 4 15 140 15 134 1.86e-68 205
MsG0780041809.01.T01 MTR_8g024180 55.479 146 49 3 25 170 27 156 2.59e-43 142
MsG0780041809.01.T01 MTR_4g013200 48.000 75 39 0 96 170 59 133 2.55e-21 85.1
MsG0780041809.01.T01 MTR_7g116795 44.000 75 42 0 92 166 69 143 4.96e-19 79.7
MsG0780041809.01.T01 MTR_5g032440 28.431 102 72 1 69 169 67 168 2.71e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041809.01.T01 AT2G46820 60.145 138 40 2 36 170 35 160 5.53e-50 159
MsG0780041809.01.T01 AT2G46820 60.145 138 40 2 36 170 35 160 5.53e-50 159
MsG0780041809.01.T01 AT1G52220 47.727 88 45 1 82 169 56 142 1.31e-22 89.0
MsG0780041809.01.T01 AT1G52220 47.727 88 44 2 82 169 56 141 8.46e-21 84.3
MsG0780041809.01.T01 AT4G01150 33.103 145 76 4 25 166 20 146 1.68e-20 83.6
MsG0780041809.01.T01 AT1G52220 46.479 71 37 1 82 152 56 125 1.18e-13 65.1

Find 63 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGGCTTCCTTTAATTCC+TGG 0.266011 7:+95382599 MsG0780041809.01.T01:CDS
TAGCAGGTACGGTTGCATTA+TGG 0.268289 7:+95382458 MsG0780041809.01.T01:CDS
TCTTCATTCACTCTTATTGA+TGG 0.283095 7:+95381956 MsG0780041809.01.T01:CDS
TGAATAGTCTTGAGAAGTTC+TGG 0.288982 7:-95382285 None:intergenic
ACAGAAAGCGGTGGTCTTCC+AGG 0.290167 7:-95382065 None:intergenic
TCAAGAACCCCAGGAATTAA+AGG 0.320013 7:-95382608 None:intergenic
AGCAGGTACGGTTGCATTAT+GGG 0.364589 7:+95382459 MsG0780041809.01.T01:CDS
ATAGGCTTCCTTTAATTCCT+GGG 0.369347 7:+95382600 MsG0780041809.01.T01:CDS
AAGAGTGAATGAAGAGAAAG+TGG 0.378344 7:-95381948 None:intergenic
TGGTCTTCCAGGGACGAGTA+TGG 0.381671 7:-95382054 None:intergenic
GTTGCATTATGGGGATCAGC+TGG 0.386926 7:+95382469 MsG0780041809.01.T01:CDS
GAAAGTGGAAGATGAGGCTG+AGG 0.422883 7:-95381933 None:intergenic
ACATAATAATCTATCTGTAT+TGG 0.431518 7:+95381877 MsG0780041809.01.T01:five_prime_UTR
CAAAGTGTGGCAATAGCATA+TGG 0.432174 7:+95381791 MsG0780041809.01.T01:five_prime_UTR
CTCGTAGGCATTGGCTACAC+TGG 0.433498 7:+95382632 MsG0780041809.01.T01:CDS
AGCCACTCATTTCTTTCATA+CGG 0.453220 7:-95381764 None:intergenic
TGATGAAACAGGCTATTGAT+AGG 0.459015 7:+95382582 MsG0780041809.01.T01:intron
CATATGCTATTGCCACACTT+TGG 0.467713 7:-95381790 None:intergenic
ACCGATCTGGCTTGAAAACT+AGG 0.468356 7:-95382770 None:intergenic
TTGCATTATGGGGATCAGCT+GGG 0.468402 7:+95382470 MsG0780041809.01.T01:CDS
TGGAGAAGCAGAGTGTCTGC+AGG 0.469794 7:-95381987 None:intergenic
AACTCAAATTAACAAGCAAA+TGG 0.489389 7:+95381907 MsG0780041809.01.T01:exon
TGGGACTGAACAGGTGGAGG+GGG 0.502807 7:-95382034 None:intergenic
TGCTCTAGCGGTAGCAGGTA+CGG 0.502934 7:+95382447 MsG0780041809.01.T01:CDS
ATCTGCCTCTGTTTCTGTTG+TGG 0.505718 7:-95382208 None:intergenic
TTCAGTCCCATACTCGTCCC+TGG 0.506421 7:+95382047 MsG0780041809.01.T01:CDS
GTTCTTGAACTCGTAGGCAT+TGG 0.511626 7:+95382623 MsG0780041809.01.T01:CDS
TTGCTTGTTAATTTGAGTTG+AGG 0.511958 7:-95381903 None:intergenic
GGACGAGTATGGGACTGAAC+AGG 0.517757 7:-95382043 None:intergenic
TGCTCGCAATGTTATGTCCA+TGG 0.518500 7:+95382176 MsG0780041809.01.T01:CDS
AGTGGAAGATGAGGCTGAGG+AGG 0.528576 7:-95381930 None:intergenic
TTTATGGTATTTCATTGGAT+AGG 0.536714 7:-95381713 None:intergenic
GGTCTTCCAGGGACGAGTAT+GGG 0.539747 7:-95382053 None:intergenic
AGAATCAGTGACAGTAGTAC+TGG 0.542464 7:-95382262 None:intergenic
GTGAGTGCTCTAGCGGTAGC+AGG 0.543399 7:+95382442 MsG0780041809.01.T01:CDS
AAGAGCATAGCATACCGATC+TGG 0.544761 7:-95382783 None:intergenic
TGCATTATGGGGATCAGCTG+GGG 0.548762 7:+95382471 MsG0780041809.01.T01:CDS
GTATGGGACTGAACAGGTGG+AGG 0.553903 7:-95382037 None:intergenic
TAGGCTTCCTTTAATTCCTG+GGG 0.554551 7:+95382601 MsG0780041809.01.T01:CDS
TGTTGTGGTACTTGAAGCCA+TGG 0.559345 7:-95382193 None:intergenic
TCGTAGGCATTGGCTACACT+GGG 0.562769 7:+95382633 MsG0780041809.01.T01:CDS
AGTTGAGGCAACCTCTGTTG+TGG 0.572988 7:-95382235 None:intergenic
TATGGGACTGAACAGGTGGA+GGG 0.576384 7:-95382036 None:intergenic
GGATAGTGTCTCTGCTGACT+TGG 0.581587 7:-95381743 None:intergenic
CAGAAAGCGGTGGTCTTCCA+GGG 0.585352 7:-95382064 None:intergenic
TGTATTTGACTTACAGAAAG+CGG 0.587004 7:-95382077 None:intergenic
CGAGTATGGGACTGAACAGG+TGG 0.598195 7:-95382040 None:intergenic
AGTAGTACTGGTGTCAGTTG+AGG 0.598742 7:-95382250 None:intergenic
ATGGGACTGAACAGGTGGAG+GGG 0.599012 7:-95382035 None:intergenic
GCAGGTACGGTTGCATTATG+GGG 0.599277 7:+95382460 MsG0780041809.01.T01:CDS
TGAAGAGAAAGTGGAAGATG+AGG 0.604075 7:-95381939 None:intergenic
GTTGGAAGGCTCACACATTG+TGG 0.610597 7:-95382007 None:intergenic
CCTGGGGTTCTTGAACTCGT+AGG 0.612235 7:+95382617 MsG0780041809.01.T01:CDS
CCTACGAGTTCAAGAACCCC+AGG 0.617642 7:-95382617 None:intergenic
GGAGAAGCAGAGTGTCTGCA+GGG 0.624844 7:-95381986 None:intergenic
GTATGCAGTGAGTGCTCTAG+CGG 0.647497 7:+95382435 MsG0780041809.01.T01:CDS
GGCAGATGCAGCCACAACAG+AGG 0.654208 7:+95382224 MsG0780041809.01.T01:CDS
TGAGTGGCTCATCCAAAGTG+TGG 0.665141 7:+95381778 MsG0780041809.01.T01:five_prime_UTR
ATCCGTATGAAAGAAATGAG+TGG 0.680937 7:+95381762 MsG0780041809.01.T01:five_prime_UTR
AAGTACCACAACAGAAACAG+AGG 0.689454 7:+95382203 MsG0780041809.01.T01:CDS
ATTTGACTTACAGAAAGCGG+TGG 0.698122 7:-95382074 None:intergenic
GAAGCAGAGTGTCTGCAGGG+AGG 0.716854 7:-95381983 None:intergenic
CGTAGGCATTGGCTACACTG+GGG 0.765097 7:+95382634 MsG0780041809.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATTATATGCAAAA+AGG - Chr7:95382517-95382536 None:intergenic 10.0%
!! ACATAATAATCTATCTGTAT+TGG + Chr7:95381877-95381896 MsG0780041809.01.T01:five_prime_UTR 20.0%
!! ATATAATTGAATTTGTGTGT+TGG + Chr7:95382130-95382149 MsG0780041809.01.T01:intron 20.0%
!! GTTAATTAGATCTACAAAAA+TGG - Chr7:95381825-95381844 None:intergenic 20.0%
!! TATATTATATGCAAAAAGGA+AGG - Chr7:95382513-95382532 None:intergenic 20.0%
! AACTCAAATTAACAAGCAAA+TGG + Chr7:95381907-95381926 MsG0780041809.01.T01:exon 25.0%
! ATAGTATATGTGTATTGAGT+TGG + Chr7:95382369-95382388 MsG0780041809.01.T01:intron 25.0%
GATTGGATGTAATGTATAGT+GGG + Chr7:95382396-95382415 MsG0780041809.01.T01:intron 30.0%
TCTTCATTCACTCTTATTGA+TGG + Chr7:95381956-95381975 MsG0780041809.01.T01:CDS 30.0%
! GCGATTTTATGGTATTTCAT+TGG - Chr7:95381721-95381740 None:intergenic 30.0%
! TGTATTTGACTTACAGAAAG+CGG - Chr7:95382080-95382099 None:intergenic 30.0%
!! ATATGTGTATTGAGTTGGAT+TGG + Chr7:95382374-95382393 MsG0780041809.01.T01:intron 30.0%
!! TTGCTTGTTAATTTGAGTTG+AGG - Chr7:95381906-95381925 None:intergenic 30.0%
AAGAGTGAATGAAGAGAAAG+TGG - Chr7:95381951-95381970 None:intergenic 35.0%
AGCCACTCATTTCTTTCATA+CGG - Chr7:95381767-95381786 None:intergenic 35.0%
ATAGGCTTCCTTTAATTCCT+GGG + Chr7:95382600-95382619 MsG0780041809.01.T01:CDS 35.0%
ATCCGTATGAAAGAAATGAG+TGG + Chr7:95381762-95381781 MsG0780041809.01.T01:five_prime_UTR 35.0%
ATCTGCAGAAGATACAAAGT+AGG - Chr7:95382666-95382685 None:intergenic 35.0%
GGATTGGATGTAATGTATAG+TGG + Chr7:95382395-95382414 MsG0780041809.01.T01:intron 35.0%
TGATGAAACAGGCTATTGAT+AGG + Chr7:95382582-95382601 MsG0780041809.01.T01:intron 35.0%
! TGAATAGTCTTGAGAAGTTC+TGG - Chr7:95382288-95382307 None:intergenic 35.0%
!! TGTATTGAGTTGGATTGGAT+TGG + Chr7:95382379-95382398 MsG0780041809.01.T01:intron 35.0%
AAGTACCACAACAGAAACAG+AGG + Chr7:95382203-95382222 MsG0780041809.01.T01:CDS 40.0%
AGAATCAGTGACAGTAGTAC+TGG - Chr7:95382265-95382284 None:intergenic 40.0%
CAAAGTGTGGCAATAGCATA+TGG + Chr7:95381791-95381810 MsG0780041809.01.T01:five_prime_UTR 40.0%
CATATGCTATTGCCACACTT+TGG - Chr7:95381793-95381812 None:intergenic 40.0%
GATAGGCTTCCTTTAATTCC+TGG + Chr7:95382599-95382618 MsG0780041809.01.T01:CDS 40.0%
TAGGCTTCCTTTAATTCCTG+GGG + Chr7:95382601-95382620 MsG0780041809.01.T01:CDS 40.0%
TCAAGAACCCCAGGAATTAA+AGG - Chr7:95382611-95382630 None:intergenic 40.0%
TCGTATCTTCTTCTTTGCAG+TGG + Chr7:95382733-95382752 MsG0780041809.01.T01:intron 40.0%
TGAAGAGAAAGTGGAAGATG+AGG - Chr7:95381942-95381961 None:intergenic 40.0%
! ATTTGACTTACAGAAAGCGG+TGG - Chr7:95382077-95382096 None:intergenic 40.0%
!! ACCTAGTTTTCAAGCCAGAT+CGG + Chr7:95382769-95382788 MsG0780041809.01.T01:CDS 40.0%
AGCAGGTACGGTTGCATTAT+GGG + Chr7:95382459-95382478 MsG0780041809.01.T01:CDS 45.0%
GCATCTGCATCTGATGAAAC+AGG + Chr7:95382571-95382590 MsG0780041809.01.T01:intron 45.0%
GTTCTTGAACTCGTAGGCAT+TGG + Chr7:95382623-95382642 MsG0780041809.01.T01:CDS 45.0%
TAGCAGGTACGGTTGCATTA+TGG + Chr7:95382458-95382477 MsG0780041809.01.T01:CDS 45.0%
TGCTCGCAATGTTATGTCCA+TGG + Chr7:95382176-95382195 MsG0780041809.01.T01:CDS 45.0%
TGTTGTGGTACTTGAAGCCA+TGG - Chr7:95382196-95382215 None:intergenic 45.0%
TTGCATTATGGGGATCAGCT+GGG + Chr7:95382470-95382489 MsG0780041809.01.T01:CDS 45.0%
! AAGAGCATAGCATACCGATC+TGG - Chr7:95382786-95382805 None:intergenic 45.0%
! ACCGATCTGGCTTGAAAACT+AGG - Chr7:95382773-95382792 None:intergenic 45.0%
! GTTGGTTGATTCACTCAGGT+CGG + Chr7:95382148-95382167 MsG0780041809.01.T01:intron 45.0%
!! AGTAGTACTGGTGTCAGTTG+AGG - Chr7:95382253-95382272 None:intergenic 45.0%
!! ATCTGCCTCTGTTTCTGTTG+TGG - Chr7:95382211-95382230 None:intergenic 45.0%
!! GTGTGTTGGTTGATTCACTC+AGG + Chr7:95382144-95382163 MsG0780041809.01.T01:intron 45.0%
!!! CTGACTTGGCTGCGATTTTA+TGG - Chr7:95381732-95381751 None:intergenic 45.0%
AGTTGAGGCAACCTCTGTTG+TGG - Chr7:95382238-95382257 None:intergenic 50.0%
GAAAGTGGAAGATGAGGCTG+AGG - Chr7:95381936-95381955 None:intergenic 50.0%
GCAGGTACGGTTGCATTATG+GGG + Chr7:95382460-95382479 MsG0780041809.01.T01:CDS 50.0%
GTATGCAGTGAGTGCTCTAG+CGG + Chr7:95382435-95382454 MsG0780041809.01.T01:CDS 50.0%
GTTGCATTATGGGGATCAGC+TGG + Chr7:95382469-95382488 MsG0780041809.01.T01:CDS 50.0%
GTTGGAAGGCTCACACATTG+TGG - Chr7:95382010-95382029 None:intergenic 50.0%
TATGGGACTGAACAGGTGGA+GGG - Chr7:95382039-95382058 None:intergenic 50.0%
TGAGTGGCTCATCCAAAGTG+TGG + Chr7:95381778-95381797 MsG0780041809.01.T01:five_prime_UTR 50.0%
TGCATTATGGGGATCAGCTG+GGG + Chr7:95382471-95382490 MsG0780041809.01.T01:CDS 50.0%
! GGATAGTGTCTCTGCTGACT+TGG - Chr7:95381746-95381765 None:intergenic 50.0%
!! TCGTAGGCATTGGCTACACT+GGG + Chr7:95382633-95382652 MsG0780041809.01.T01:CDS 50.0%
ACAGAAAGCGGTGGTCTTCC+AGG - Chr7:95382068-95382087 None:intergenic 55.0%
AGTGGAAGATGAGGCTGAGG+AGG - Chr7:95381933-95381952 None:intergenic 55.0%
ATGGGACTGAACAGGTGGAG+GGG - Chr7:95382038-95382057 None:intergenic 55.0%
CAGAAAGCGGTGGTCTTCCA+GGG - Chr7:95382067-95382086 None:intergenic 55.0%
CCTACGAGTTCAAGAACCCC+AGG - Chr7:95382620-95382639 None:intergenic 55.0%
CCTGGGGTTCTTGAACTCGT+AGG + Chr7:95382617-95382636 MsG0780041809.01.T01:CDS 55.0%
CGAGTATGGGACTGAACAGG+TGG - Chr7:95382043-95382062 None:intergenic 55.0%
GGACGAGTATGGGACTGAAC+AGG - Chr7:95382046-95382065 None:intergenic 55.0%
GGTCTTCCAGGGACGAGTAT+GGG - Chr7:95382056-95382075 None:intergenic 55.0%
GTATGGGACTGAACAGGTGG+AGG - Chr7:95382040-95382059 None:intergenic 55.0%
TGCTCTAGCGGTAGCAGGTA+CGG + Chr7:95382447-95382466 MsG0780041809.01.T01:CDS 55.0%
TGGTCTTCCAGGGACGAGTA+TGG - Chr7:95382057-95382076 None:intergenic 55.0%
TTCAGTCCCATACTCGTCCC+TGG + Chr7:95382047-95382066 MsG0780041809.01.T01:CDS 55.0%
! GGAGAAGCAGAGTGTCTGCA+GGG - Chr7:95381989-95382008 None:intergenic 55.0%
! TGGAGAAGCAGAGTGTCTGC+AGG - Chr7:95381990-95382009 None:intergenic 55.0%
!! CGTAGGCATTGGCTACACTG+GGG + Chr7:95382634-95382653 MsG0780041809.01.T01:CDS 55.0%
!! CTCGTAGGCATTGGCTACAC+TGG + Chr7:95382632-95382651 MsG0780041809.01.T01:CDS 55.0%
ACAGGTGGAGGGGGAAGAGT+TGG - Chr7:95382028-95382047 None:intergenic 60.0%
GGCAGATGCAGCCACAACAG+AGG + Chr7:95382224-95382243 MsG0780041809.01.T01:CDS 60.0%
GTGAGTGCTCTAGCGGTAGC+AGG + Chr7:95382442-95382461 MsG0780041809.01.T01:CDS 60.0%
GTGGAGGGGGAAGAGTTGGA+AGG - Chr7:95382024-95382043 None:intergenic 60.0%
TGGGACTGAACAGGTGGAGG+GGG - Chr7:95382037-95382056 None:intergenic 60.0%
! GAAGCAGAGTGTCTGCAGGG+AGG - Chr7:95381986-95382005 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 95381716 95382827 95381716 ID=MsG0780041809.01;Name=MsG0780041809.01
Chr7 mRNA 95381716 95382827 95381716 ID=MsG0780041809.01.T01;Parent=MsG0780041809.01;Name=MsG0780041809.01.T01;_AED=0.46;_eAED=0.47;_QI=210|1|0.8|1|1|1|5|0|177
Chr7 exon 95381716 95382085 95381716 ID=MsG0780041809.01.T01:exon:20996;Parent=MsG0780041809.01.T01
Chr7 exon 95382166 95382314 95382166 ID=MsG0780041809.01.T01:exon:20997;Parent=MsG0780041809.01.T01
Chr7 exon 95382415 95382501 95382415 ID=MsG0780041809.01.T01:exon:20998;Parent=MsG0780041809.01.T01
Chr7 exon 95382593 95382655 95382593 ID=MsG0780041809.01.T01:exon:20999;Parent=MsG0780041809.01.T01
Chr7 exon 95382753 95382827 95382753 ID=MsG0780041809.01.T01:exon:21000;Parent=MsG0780041809.01.T01
Chr7 five_prime_UTR 95381716 95381925 95381716 ID=MsG0780041809.01.T01:five_prime_utr;Parent=MsG0780041809.01.T01
Chr7 CDS 95381926 95382085 95381926 ID=MsG0780041809.01.T01:cds;Parent=MsG0780041809.01.T01
Chr7 CDS 95382166 95382314 95382166 ID=MsG0780041809.01.T01:cds;Parent=MsG0780041809.01.T01
Chr7 CDS 95382415 95382501 95382415 ID=MsG0780041809.01.T01:cds;Parent=MsG0780041809.01.T01
Chr7 CDS 95382593 95382655 95382593 ID=MsG0780041809.01.T01:cds;Parent=MsG0780041809.01.T01
Chr7 CDS 95382753 95382827 95382753 ID=MsG0780041809.01.T01:cds;Parent=MsG0780041809.01.T01
Gene Sequence

>MsG0780041809.01.T01

ATGGCCTCCTCAGCCTCATCTTCCACTTTCTCTTCATTCACTCTTATTGATGGAAAGCCTCCCTGCAGACACTCTGCTTCTCCACAATGTGTGAGCCTTCCAACTCTTCCCCCTCCACCTGTTCAGTCCCATACTCGTCCCTGGAAGACCACCGCTTTCTGTCGGAAGATTGCTCGCAATGTTATGTCCATGGCTTCAAGTACCACAACAGAAACAGAGGCAGATGCAGCCACAACAGAGGTTGCCTCAACTGACACCAGTACTACTGTCACTGATTCTCCAGAACTTCTCAAGACTATTCAACAAACTTGGGATAAAGTTGAAGACAAGTATGCAGTGAGTGCTCTAGCGGTAGCAGGTACGGTTGCATTATGGGGATCAGCTGGGGTGATCTCAGCTATTGATAGGCTTCCTTTAATTCCTGGGGTTCTTGAACTCGTAGGCATTGGCTACACTGGGTGGTTTGCATACAAGAACCTAGTTTTCAAGCCAGATCGGTATGCTATGCTCTTCTTCAATAATCTGTTTCCATAA

Protein sequence

>MsG0780041809.01.T01

MASSASSSTFSSFTLIDGKPPCRHSASPQCVSLPTLPPPPVQSHTRPWKTTAFCRKIARNVMSMASSTTTETEADAATTEVASTDTSTTVTDSPELLKTIQQTWDKVEDKYAVSALAVAGTVALWGSAGVISAIDRLPLIPGVLELVGIGYTGWFAYKNLVFKPDRYAMLFFNNLFP*