AlfalfaGEDB | MsG0480022710.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022710.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022710.01.T01	XP_024637912.1	100	60	0	0	1	60	1	60	1.46E-33	131

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022710.01.T01	A0A072UPJ0	100.000	60	0	0	1	60	1	60	7.68e-34	131

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048058.01	MsG0480022710.01	0.809519	1.818240e-50	1.069839e-47
MsG0080048602.01	MsG0480022710.01	0.831730	1.414395e-55	1.548460e-52
MsG0180000101.01	MsG0480022710.01	0.828876	7.044986e-55	7.091379e-52
MsG0180000146.01	MsG0480022710.01	0.830906	2.256026e-55	2.409980e-52
MsG0180000494.01	MsG0480022710.01	0.810478	1.129864e-50	6.817862e-48
MsG0180000555.01	MsG0480022710.01	0.819262	1.273526e-52	9.752223e-50
MsG0180000741.01	MsG0480022710.01	0.839440	1.582836e-57	2.186040e-54
MsG0180001173.01	MsG0480022710.01	0.815377	9.538644e-52	6.565347e-49
MsG0180001570.01	MsG0480022710.01	0.810001	1.431996e-50	8.533994e-48
MsG0180002071.01	MsG0480022710.01	0.807862	4.109036e-50	2.313958e-47
MsG0180002300.01	MsG0480022710.01	0.808171	3.531114e-50	2.004983e-47
MsG0180002975.01	MsG0480022710.01	0.820692	5.995991e-53	4.777612e-50
MsG0180003181.01	MsG0480022710.01	0.802171	6.366482e-49	3.097491e-46
MsG0180003303.01	MsG0480022710.01	0.847530	1.090859e-59	1.944884e-56
MsG0180004056.01	MsG0480022710.01	0.817741	2.817710e-52	2.069199e-49
MsG0180004139.01	MsG0480022710.01	0.800372	1.486456e-48	6.909998e-46
MsG0180004383.01	MsG0480022710.01	0.803896	2.801001e-49	1.424305e-46
MsG0180004437.01	MsG0480022710.01	0.830489	2.853655e-55	3.010817e-52
MsG0180004598.01	MsG0480022710.01	0.828951	6.758409e-55	6.818159e-52
MsG0180004645.01	MsG0480022710.01	0.802773	4.785345e-49	2.364266e-46
MsG0180005228.01	MsG0480022710.01	0.814285	1.665593e-51	1.112968e-48
MsG0180005985.01	MsG0480022710.01	0.841231	5.386763e-58	7.863497e-55
MsG0280006367.01	MsG0480022710.01	0.802825	4.668546e-49	2.309741e-46
MsG0280010735.01	MsG0480022710.01	0.829335	5.452848e-55	5.564790e-52
MsG0280010816.01	MsG0480022710.01	0.812040	5.178283e-51	3.256977e-48
MsG0280010866.01	MsG0480022710.01	0.816866	4.433908e-52	3.178876e-49
MsG0280010932.01	MsG0480022710.01	0.824751	6.812789e-54	6.089812e-51
MsG0280010976.01	MsG0480022710.01	0.818093	2.346781e-52	1.739895e-49
MsG0280011075.01	MsG0480022710.01	0.833814	4.296514e-56	5.004324e-53
MsG0280011293.01	MsG0480022710.01	0.810338	1.211372e-50	7.282437e-48
MsG0280011336.01	MsG0480022710.01	0.823428	1.392471e-53	1.198565e-50
MsG0380011721.01	MsG0480022710.01	0.806794	6.918648e-50	3.789682e-47
MsG0380014489.01	MsG0480022710.01	0.801525	8.640089e-49	4.135301e-46
MsG0380014576.01	MsG0480022710.01	0.822789	1.963252e-53	1.659731e-50
MsG0480021204.01	MsG0480022710.01	0.801877	7.316768e-49	3.533493e-46
MsG0480021216.01	MsG0480022710.01	0.818322	2.081978e-52	1.553464e-49
MsG0480022318.01	MsG0480022710.01	0.811615	6.407269e-51	3.984542e-48
MsG0480022552.01	MsG0480022710.01	0.832583	8.700522e-56	9.767206e-53
MsG0480022678.01	MsG0480022710.01	0.803999	2.665599e-49	1.359039e-46
MsG0480022710.01	MsG0480022717.01	0.807697	4.452706e-50	2.496820e-47
MsG0480022710.01	MsG0480022719.01	0.829038	6.435583e-55	6.509131e-52
MsG0480022710.01	MsG0580024770.01	0.806129	9.554689e-50	5.145439e-47
MsG0480022710.01	MsG0580025128.01	0.826041	3.372072e-54	3.126857e-51
MsG0480022710.01	MsG0580025184.01	0.813847	2.080813e-51	1.373968e-48
MsG0480022710.01	MsG0580025907.01	0.801496	8.761922e-49	4.190583e-46
MsG0480022710.01	MsG0680034757.01	0.824869	6.387596e-54	5.728986e-51
MsG0480022710.01	MsG0780036836.01	0.805169	1.519725e-49	7.984842e-47
MsG0480022710.01	MsG0780039587.01	0.834094	3.655559e-56	4.293557e-53
MsG0480022710.01	MsG0780039717.01	0.803400	3.549241e-49	1.781901e-46
MsG0480022710.01	MsG0780040687.01	0.824604	7.376481e-54	6.566006e-51
MsG0480022710.01	MsG0780040742.01	0.807355	5.264655e-50	2.925964e-47
MsG0480022710.01	MsG0780041310.01	0.803097	4.101983e-49	2.043246e-46
MsG0480022710.01	MsG0780041371.01	0.816462	5.463002e-52	3.873276e-49
MsG0480022710.01	MsG0880042329.01	0.812577	3.954010e-51	2.523247e-48
MsG0480022710.01	MsG0880042983.01	0.839514	1.514424e-57	2.096205e-54
MsG0480022710.01	MsG0880043341.01	0.818130	2.301136e-52	1.707915e-49
MsG0480022710.01	MsG0880043533.01	0.809861	1.534998e-50	9.113848e-48
MsG0480022710.01	MsG0880044917.01	0.823539	1.312055e-53	1.132896e-50
MsG0480022710.01	MsG0880045165.01	0.813228	2.846418e-51	1.848121e-48
MsG0480022710.01	MsG0880045841.01	0.817954	2.521858e-52	1.862706e-49
MsG0480022710.01	MsG0880046643.01	0.820111	8.151892e-53	6.393051e-50
MsG0480022710.01	MsG0880047745.01	0.809156	2.175253e-50	1.267592e-47
MsG0380015436.01	MsG0480022710.01	0.826222	3.054029e-54	2.846545e-51
MsG0380016589.01	MsG0480022710.01	0.808096	3.663573e-50	2.076005e-47
MsG0380016753.01	MsG0480022710.01	0.802244	6.149370e-49	2.997497e-46
MsG0380017045.01	MsG0480022710.01	0.820697	5.982493e-53	4.767572e-50
MsG0380017077.01	MsG0480022710.01	0.804158	2.470791e-49	1.264819e-46
MsG0380017939.01	MsG0480022710.01	0.800205	1.607577e-48	7.441116e-46
MsG0280007048.01	MsG0480022710.01	0.820609	6.266728e-53	4.981716e-50
MsG0280007087.01	MsG0480022710.01	0.827745	1.320417e-54	1.286108e-51
MsG0280007726.01	MsG0480022710.01	0.815648	8.299780e-52	5.755720e-49
MsG0280007819.01	MsG0480022710.01	0.807783	4.269541e-50	2.399525e-47
MsG0280010153.01	MsG0480022710.01	0.803157	3.986463e-49	1.988886e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022710.01.T01	MTR_4g094378	100.000	60	0	0	1	60	1	60	1.95e-37	131

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022710.01.T01	AT1G09980	48.333	60	29	1	1	60	1	58	5.81e-13	61.6
MsG0480022710.01.T01	AT1G09980	48.333	60	29	1	1	60	1	58	5.81e-13	61.6
MsG0480022710.01.T01	AT1G09980	48.333	60	29	1	1	60	1	58	6.04e-13	61.6
MsG0480022710.01.T01	AT1G58350	46.667	60	30	1	1	60	1	58	1.41e-12	60.5
MsG0480022710.01.T01	AT1G58350	46.667	60	30	1	1	60	1	58	1.41e-12	60.5
MsG0480022710.01.T01	AT1G58350	46.667	60	30	1	1	60	1	58	1.41e-12	60.5
MsG0480022710.01.T01	AT1G58350	46.667	60	30	1	1	60	1	58	1.50e-12	60.5

Find 13 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATTTGTCAACTAAA+AGG	0.258176	4:-75730138	MsG0480022710.01.T01:CDS
GGCAAGTGATTTGGCCTTGT+TGG	0.331290	4:+75730091	None:intergenic
GCAAGTGATTTGGCCTTGTT+GGG	0.350558	4:+75730092	None:intergenic
CGGAGATTTAGATGGTTTGT+TGG	0.388934	4:-75730169	MsG0480022710.01.T01:CDS
TCAAGGTTAGGCAAGTGATT+TGG	0.403889	4:+75730082	None:intergenic
ATATGTTTCGGAGATTTAGA+TGG	0.411888	4:-75730177	MsG0480022710.01.T01:CDS
ACTTACTGCTACATATGTTT+CGG	0.420808	4:-75730189	None:intergenic
TGATTTGGCCTTGTTGGGCC+AGG	0.471182	4:+75730097	None:intergenic
CAACTTCATGAACAGCATCA+AGG	0.534210	4:+75730065	None:intergenic
GAATTTGTCAACTAAAAGGT+TGG	0.552284	4:-75730134	MsG0480022710.01.T01:CDS
TTGGTCAATGCTGATCAACC+TGG	0.571852	4:-75730115	MsG0480022710.01.T01:CDS
TCATGAACAGCATCAAGGTT+AGG	0.591446	4:+75730070	None:intergenic
CTGATCAACCTGGCCCAACA+AGG	0.604918	4:-75730105	MsG0480022710.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAGAATTTGTCAACTAAA+AGG	-	Chr4:75730052-75730071	MsG0480022710.01.T01:CDS	20.0%
!!	AATCTTGATCTTTTTGAACA+AGG	-	Chr4:75730171-75730190	MsG0480022710.01.T01:CDS	25.0%
!!	ATCTTGATCTTTTTGAACAA+GGG	-	Chr4:75730172-75730191	MsG0480022710.01.T01:CDS	25.0%
	GAATTTGTCAACTAAAAGGT+TGG	-	Chr4:75730056-75730075	MsG0480022710.01.T01:CDS	30.0%
	CAACTTCATGAACAGCATCA+AGG	+	Chr4:75730128-75730147	None:intergenic	40.0%
	TCAAGGTTAGGCAAGTGATT+TGG	+	Chr4:75730111-75730130	None:intergenic	40.0%
	TCATGAACAGCATCAAGGTT+AGG	+	Chr4:75730123-75730142	None:intergenic	40.0%
!	CGGAGATTTAGATGGTTTGT+TGG	-	Chr4:75730021-75730040	MsG0480022710.01.T01:CDS	40.0%
	TTGGTCAATGCTGATCAACC+TGG	-	Chr4:75730075-75730094	MsG0480022710.01.T01:CDS	45.0%
!!	GCAAGTGATTTGGCCTTGTT+GGG	+	Chr4:75730101-75730120	None:intergenic	45.0%
!!	GGCAAGTGATTTGGCCTTGT+TGG	+	Chr4:75730102-75730121	None:intergenic	50.0%
	CTGATCAACCTGGCCCAACA+AGG	-	Chr4:75730085-75730104	MsG0480022710.01.T01:CDS	55.0%
!!	TGATTTGGCCTTGTTGGGCC+AGG	+	Chr4:75730096-75730115	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	75730015	75730197	75730015	ID=MsG0480022710.01;Name=MsG0480022710.01
Chr4	mRNA	75730015	75730197	75730015	ID=MsG0480022710.01.T01;Parent=MsG0480022710.01;Name=MsG0480022710.01.T01;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|60
Chr4	exon	75730015	75730197	75730015	ID=MsG0480022710.01.T01:exon:4777;Parent=MsG0480022710.01.T01
Chr4	CDS	75730015	75730197	75730015	ID=MsG0480022710.01.T01:cds;Parent=MsG0480022710.01.T01

Gene Sequence	>MsG0480022710.01.T01 ATGTTTCGGAGATTTAGATGGTTTGTTGGTTTGAATCAAAAGAATTTGTCAACTAAAAGGTTGGTCAATGCTGATCAACCTGGCCCAACAAGGCCAAATCACTTGCCTAACCTTGATGCTGTTCATGAAGTTGCAATTTATATTCATAGATTTCATAATCTTGATCTTTTTGAACAAGGGTAA
Protein sequence	>MsG0480022710.01.T01 MFRRFRWFVGLNQKNLSTKRLVNADQPGPTRPNHLPNLDAVHEVAIYIHRFHNLDLFEQG*