AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002300.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002300.01.T02 MTR_2g039140 40.132 152 74 2 1 135 120 271 2.60e-25 99.8
MsG0180002300.01.T02 MTR_4g036430 89.744 39 4 0 1 39 31 69 1.27e-20 82.0
MsG0180002300.01.T01 MTR_2g039140 47.934 121 56 1 31 144 151 271 3.60e-30 112
MsG0180002300.01.T01 MTR_4g036430 89.744 39 4 0 1 39 31 69 6.22e-20 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002300.01.T02 AT3G29785 57.778 90 37 1 11 100 7 95 1.22e-32 114
MsG0180002300.01.T01 AT3G29785 57.778 90 37 1 11 100 7 95 1.32e-32 114

Find 21 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGAACTACGATGATTTC+TGG 0.189947 1:-36434761 None:intergenic
ACTACGATGATTTCTGGATT+TGG 0.314338 1:-36434755 None:intergenic
ATTTCTGGATTTGGATTGTC+TGG 0.336440 1:-36434746 None:intergenic
AGAAATTCAACCAGAGGAAA+TGG 0.417568 1:+36434706 MsG0180002300.01.T02:CDS
TGTCTTCCCTACCAGATAGT+TGG 0.424640 1:+36434533 MsG0180002300.01.T02:intron
GAGATCCGACGGAGAGAGTT+GGG 0.442215 1:+36434640 MsG0180002300.01.T02:CDS
ATTTGATGATGTTCGTGATC+TGG 0.482957 1:+36434606 MsG0180002300.01.T02:CDS
TTTACCACGTCCATTTCCTC+TGG 0.487326 1:-36434716 None:intergenic
TCTATCGAGTGTTGGAATTG+TGG 0.512167 1:+36434793 MsG0180002300.01.T02:CDS
CACAGCTGTGAGTAGCTCCT+CGG 0.527844 1:+36434567 MsG0180002300.01.T02:intron
ACAGCTGTGAGTAGCTCCTC+GGG 0.532462 1:+36434568 MsG0180002300.01.T02:intron
AGAGATCCGACGGAGAGAGT+TGG 0.549236 1:+36434639 MsG0180002300.01.T02:CDS
ACTCGAAATCTATCGAGTGT+TGG 0.561027 1:+36434785 MsG0180002300.01.T02:CDS
CTCTGTATGCAATACTGAAG+AGG 0.567853 1:-36434674 None:intergenic
AAATGGACGTGGTAAATCAA+AGG 0.596335 1:+36434723 MsG0180002300.01.T02:CDS
ATTCACCCAACTCTCTCCGT+CGG 0.618585 1:-36434645 None:intergenic
TACTCAGTGAAGAGATCCGA+CGG 0.629113 1:+36434629 MsG0180002300.01.T02:CDS
AGAGGAAGAAATTCAACCAG+AGG 0.643795 1:+36434700 MsG0180002300.01.T02:CDS
TCAACCAGAGGAAATGGACG+TGG 0.665050 1:+36434712 MsG0180002300.01.T02:CDS
GTATTGCATACAGAGTCAAG+AGG 0.682691 1:+36434682 MsG0180002300.01.T02:CDS
GTTGGAATTGTGGAAAGACG+AGG 0.688365 1:+36434803 MsG0180002300.01.T02:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAATAAAGTTCATTTGATG+AGG + Chr1:36434380-36434399 MsG0180002300.01.T02:CDS 25.0%
ATAAAGTTCATTTGATGAGG+CGG + Chr1:36434383-36434402 MsG0180002300.01.T02:CDS 30.0%
GATACCAACTGAACTTAATT+GGG - Chr1:36434479-36434498 None:intergenic 30.0%
GTGAAAATTGAAAAGTTCGA+TGG + Chr1:36434112-36434131 MsG0180002300.01.T02:CDS 30.0%
! TGAACTTTATTTGATGCTGA+AGG - Chr1:36434373-36434392 None:intergenic 30.0%
AAAGAAACCAGAATCGATGA+AGG + Chr1:36434210-36434229 MsG0180002300.01.T02:CDS 35.0%
AAATGGACGTGGTAAATCAA+AGG + Chr1:36434723-36434742 MsG0180002300.01.T02:CDS 35.0%
AATTGAAAAGTTCGATGGTG+CGG + Chr1:36434117-36434136 MsG0180002300.01.T02:CDS 35.0%
ACAACCCAATTAAGTTCAGT+TGG + Chr1:36434472-36434491 MsG0180002300.01.T02:intron 35.0%
AGAAATTCAACCAGAGGAAA+TGG + Chr1:36434706-36434725 MsG0180002300.01.T02:CDS 35.0%
CGATACCAACTGAACTTAAT+TGG - Chr1:36434480-36434499 None:intergenic 35.0%
! ACTACGATGATTTCTGGATT+TGG - Chr1:36434758-36434777 None:intergenic 35.0%
! ATTTCTGGATTTGGATTGTC+TGG - Chr1:36434749-36434768 None:intergenic 35.0%
!! ATTTGATGATGTTCGTGATC+TGG + Chr1:36434606-36434625 MsG0180002300.01.T02:CDS 35.0%
AATCGATGAAGGATGATGAG+TGG + Chr1:36434221-36434240 MsG0180002300.01.T02:CDS 40.0%
ACTCGAAATCTATCGAGTGT+TGG + Chr1:36434785-36434804 MsG0180002300.01.T02:CDS 40.0%
AGAGGAAGAAATTCAACCAG+AGG + Chr1:36434700-36434719 MsG0180002300.01.T02:CDS 40.0%
CTCTGTATGCAATACTGAAG+AGG - Chr1:36434677-36434696 None:intergenic 40.0%
CTGAGAACTACGATGATTTC+TGG - Chr1:36434764-36434783 None:intergenic 40.0%
GTATTGCATACAGAGTCAAG+AGG + Chr1:36434682-36434701 MsG0180002300.01.T02:CDS 40.0%
TTTACACTTCGGATGACAGA+AGG + Chr1:36434409-36434428 MsG0180002300.01.T02:CDS 40.0%
! TGGTATCGAATTTGACGATG+AGG + Chr1:36434492-36434511 MsG0180002300.01.T02:intron 40.0%
! TTTCATCTTTTTGCTTCCCG+AGG - Chr1:36434587-36434606 None:intergenic 40.0%
!! TCATCATCCTTCATCGATTC+TGG - Chr1:36434220-36434239 None:intergenic 40.0%
!! TCTATCGAGTGTTGGAATTG+TGG + Chr1:36434793-36434812 MsG0180002300.01.T02:CDS 40.0%
!! TCTGGTTTCTTTTCTGTGAG+AGG - Chr1:36434202-36434221 None:intergenic 40.0%
AAGAGCCTTCATAAGACCAG+CGG - Chr1:36434332-36434351 None:intergenic 45.0%
ACAGTAGCACTCCAACTATC+TGG - Chr1:36434547-36434566 None:intergenic 45.0%
AGCACTCCAACTATCTGGTA+GGG - Chr1:36434542-36434561 None:intergenic 45.0%
CAAGTGCTTGTCTATCGAGA+AGG - Chr1:36434249-36434268 None:intergenic 45.0%
CTTCTCGATAGACAAGCACT+TGG + Chr1:36434247-36434266 MsG0180002300.01.T02:CDS 45.0%
GTTGGAATTGTGGAAAGACG+AGG + Chr1:36434803-36434822 MsG0180002300.01.T02:CDS 45.0%
TACTCAGTGAAGAGATCCGA+CGG + Chr1:36434629-36434648 MsG0180002300.01.T02:CDS 45.0%
TAGCACTCCAACTATCTGGT+AGG - Chr1:36434543-36434562 None:intergenic 45.0%
TGAGGCGGTTGTTTACACTT+CGG + Chr1:36434398-36434417 MsG0180002300.01.T02:CDS 45.0%
TGTCTTCCCTACCAGATAGT+TGG + Chr1:36434533-36434552 MsG0180002300.01.T02:intron 45.0%
TTTACCACGTCCATTTCCTC+TGG - Chr1:36434719-36434738 None:intergenic 45.0%
AAGTTCGATGGTGCGGACTT+TGG + Chr1:36434124-36434143 MsG0180002300.01.T02:CDS 50.0%
ATTCACCCAACTCTCTCCGT+CGG - Chr1:36434648-36434667 None:intergenic 50.0%
GCAAAAGAGAAAACCACCGC+TGG + Chr1:36434313-36434332 MsG0180002300.01.T02:CDS 50.0%
TCAACCAGAGGAAATGGACG+TGG + Chr1:36434712-36434731 MsG0180002300.01.T02:CDS 50.0%
!! AACCACCGCTGGTCTTATGA+AGG + Chr1:36434324-36434343 MsG0180002300.01.T02:CDS 50.0%
!!! ATGGTGCGGACTTTGGCTTT+TGG + Chr1:36434131-36434150 MsG0180002300.01.T02:CDS 50.0%
ACAGCTGTGAGTAGCTCCTC+GGG + Chr1:36434568-36434587 MsG0180002300.01.T02:intron 55.0%
AGAGATCCGACGGAGAGAGT+TGG + Chr1:36434639-36434658 MsG0180002300.01.T02:CDS 55.0%
AGCCTTCATAAGACCAGCGG+TGG - Chr1:36434329-36434348 None:intergenic 55.0%
CACAGCTGTGAGTAGCTCCT+CGG + Chr1:36434567-36434586 MsG0180002300.01.T02:intron 55.0%
GAGATCCGACGGAGAGAGTT+GGG + Chr1:36434640-36434659 MsG0180002300.01.T02:CDS 55.0%
! GATGACAGAAGGCGCATCAG+TGG + Chr1:36434420-36434439 MsG0180002300.01.T02:intron 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36434091 36434846 36434091 ID=MsG0180002300.01;Name=MsG0180002300.01
Chr1 mRNA 36434091 36434846 36434091 ID=MsG0180002300.01.T01;Parent=MsG0180002300.01;Name=MsG0180002300.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|212
Chr1 exon 36434548 36434846 36434548 ID=MsG0180002300.01.T01:exon:237;Parent=MsG0180002300.01.T01
Chr1 CDS 36434091 36434430 36434091 ID=MsG0180002300.01.T01:cds;Parent=MsG0180002300.01.T01
Chr1 CDS 36434548 36434846 36434548 ID=MsG0180002300.01.T01:cds;Parent=MsG0180002300.01.T01
Chr1 mRNA 36434091 36434846 36434091 ID=MsG0180002300.01.T02;Parent=MsG0180002300.01;Name=MsG0180002300.01.T02;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|203
Chr1 exon 36434575 36434846 36434575 ID=MsG0180002300.01.T02:exon:238;Parent=MsG0180002300.01.T02
Chr1 CDS 36434091 36434430 36434091 ID=MsG0180002300.01.T02:cds;Parent=MsG0180002300.01.T02
Chr1 CDS 36434575 36434846 36434575 ID=MsG0180002300.01.T02:cds;Parent=MsG0180002300.01.T02
Gene Sequence

>MsG0180002300.01.T02

ATGGCTGCAGACGAAGGAAAGGTGAAAATTGAAAAGTTCGATGGTGCGGACTTTGGCTTTTGGAAGATGCAGATCGAAGATTATTTGTATCAGAAAAAGCTACATCAACCTCTCACAGAAAAGAAACCAGAATCGATGAAGGATGATGAGTGGAACCTTCTCGATAGACAAGCACTTGGTGTTGTGCGATTGTCGTTATCTCGAAATGTTGCTTTTAACATAGCAAAAGAGAAAACCACCGCTGGTCTTATGAAGGCTCTTTCTAGCATGTACGAAAAGCCTTCAGCATCAAATAAAGTTCATTTGATGAGGCGGTTGTTTACACTTCGGATGACAGAAGTGAGTAGCTCCTCGGGAAGCAAAAAGATGAAATTTGATGATGTTCGTGATCTGGTACTCAGTGAAGAGATCCGACGGAGAGAGTTGGGTGAATCTTCATCCTCTTCAGTATTGCATACAGAGTCAAGAGGAAGAAATTCAACCAGAGGAAATGGACGTGGTAAATCAAAGGCCAGACAATCCAAATCCAGAAATCATCGTAGTTCTCAGAACTCGAAATCTATCGAGTGTTGGAATTGTGGAAAGACGAGGACACTTCAAAAATCAGTGTAG

>MsG0180002300.01.T01

ATGGCTGCAGACGAAGGAAAGGTGAAAATTGAAAAGTTCGATGGTGCGGACTTTGGCTTTTGGAAGATGCAGATCGAAGATTATTTGTATCAGAAAAAGCTACATCAACCTCTCACAGAAAAGAAACCAGAATCGATGAAGGATGATGAGTGGAACCTTCTCGATAGACAAGCACTTGGTGTTGTGCGATTGTCGTTATCTCGAAATGTTGCTTTTAACATAGCAAAAGAGAAAACCACCGCTGGTCTTATGAAGGCTCTTTCTAGCATGTACGAAAAGCCTTCAGCATCAAATAAAGTTCATTTGATGAGGCGGTTGTTTACACTTCGGATGACAGAAGATAGTTGGAGTGCTACTGTCACAGCTGTGAGTAGCTCCTCGGGAAGCAAAAAGATGAAATTTGATGATGTTCGTGATCTGGTACTCAGTGAAGAGATCCGACGGAGAGAGTTGGGTGAATCTTCATCCTCTTCAGTATTGCATACAGAGTCAAGAGGAAGAAATTCAACCAGAGGAAATGGACGTGGTAAATCAAAGGCCAGACAATCCAAATCCAGAAATCATCGTAGTTCTCAGAACTCGAAATCTATCGAGTGTTGGAATTGTGGAAAGACGAGGACACTTCAAAAATCAGTGTAG

Protein sequence

>MsG0180002300.01.T02

MAADEGKVKIEKFDGADFGFWKMQIEDYLYQKKLHQPLTEKKPESMKDDEWNLLDRQALGVVRLSLSRNVAFNIAKEKTTAGLMKALSSMYEKPSASNKVHLMRRLFTLRMTEVSSSSGSKKMKFDDVRDLVLSEEIRRRELGESSSSSVLHTESRGRNSTRGNGRGKSKARQSKSRNHRSSQNSKSIECWNCGKTRTLQKSV*

>MsG0180002300.01.T01

MAADEGKVKIEKFDGADFGFWKMQIEDYLYQKKLHQPLTEKKPESMKDDEWNLLDRQALGVVRLSLSRNVAFNIAKEKTTAGLMKALSSMYEKPSASNKVHLMRRLFTLRMTEDSWSATVTAVSSSSGSKKMKFDDVRDLVLSEEIRRRELGESSSSSVLHTESRGRNSTRGNGRGKSKARQSKSRNHRSSQNSKSIECWNCGKTRTLQKSV*