Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023136.01.T01 | PNY12828.1 | 85.714 | 63 | 9 | 0 | 1 | 63 | 91 | 153 | 1.31E-29 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023136.01.T01 | O04147 | 62.069 | 58 | 22 | 0 | 1 | 58 | 117 | 174 | 1.96E-19 | 80.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023136.01.T01 | A0A2K3PC03 | 85.714 | 63 | 9 | 0 | 1 | 63 | 91 | 153 | 6.26e-30 | 113 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001599.01 | MsG0480023136.01 | -0.805738 | 1.154994e-49 | 6.158181e-47 |
| MsG0180001886.01 | MsG0480023136.01 | -0.804055 | 2.595769e-49 | 1.325341e-46 |
| MsG0180002140.01 | MsG0480023136.01 | -0.801877 | 7.316583e-49 | 3.533411e-46 |
| MsG0180003379.01 | MsG0480023136.01 | -0.801583 | 8.409861e-49 | 4.030789e-46 |
| MsG0180004016.01 | MsG0480023136.01 | -0.840345 | 9.196213e-58 | 1.306085e-54 |
| MsG0180004599.01 | MsG0480023136.01 | -0.807774 | 4.289909e-50 | 2.410343e-47 |
| MsG0180005106.01 | MsG0480023136.01 | -0.816808 | 4.568378e-52 | 3.269973e-49 |
| MsG0180005303.01 | MsG0480023136.01 | 0.810107 | 1.358842e-50 | 8.120261e-48 |
| MsG0180005999.01 | MsG0480023136.01 | -0.829567 | 4.789017e-55 | 4.919731e-52 |
| MsG0180006050.01 | MsG0480023136.01 | -0.812484 | 4.141485e-51 | 2.636565e-48 |
| MsG0280006306.01 | MsG0480023136.01 | 0.812932 | 3.306044e-51 | 2.129455e-48 |
| MsG0280006411.01 | MsG0480023136.01 | -0.826490 | 2.636955e-54 | 2.476474e-51 |
| MsG0280011287.01 | MsG0480023136.01 | -0.819326 | 1.231962e-52 | 9.449980e-50 |
| MsG0380013181.01 | MsG0480023136.01 | 0.812155 | 4.888801e-51 | 3.084773e-48 |
| MsG0480018847.01 | MsG0480023136.01 | -0.840222 | 9.902340e-58 | 1.401162e-54 |
| MsG0480020327.01 | MsG0480023136.01 | 0.866260 | 3.248259e-65 | 1.087918e-61 |
| MsG0480021041.01 | MsG0480023136.01 | -0.813634 | 2.317269e-51 | 1.521343e-48 |
| MsG0480021876.01 | MsG0480023136.01 | 0.828924 | 6.858426e-55 | 6.913704e-52 |
| MsG0480021994.01 | MsG0480023136.01 | -0.802278 | 6.052756e-49 | 2.952906e-46 |
| MsG0480022246.01 | MsG0480023136.01 | -0.817029 | 4.076589e-52 | 2.935768e-49 |
| MsG0480022493.01 | MsG0480023136.01 | -0.803188 | 3.926506e-49 | 1.960568e-46 |
| MsG0480023136.01 | MsG0480023415.01 | -0.802304 | 5.977340e-49 | 2.918205e-46 |
| MsG0480023136.01 | MsG0480023841.01 | 0.806195 | 9.256263e-50 | 4.993102e-47 |
| MsG0480023136.01 | MsG0580024581.01 | -0.805231 | 1.475049e-49 | 7.762312e-47 |
| MsG0480023136.01 | MsG0580025400.01 | -0.806460 | 8.137368e-50 | 4.419379e-47 |
| MsG0480023136.01 | MsG0580025756.01 | -0.807584 | 4.706703e-50 | 2.631338e-47 |
| MsG0480023136.01 | MsG0580026225.01 | -0.801564 | 8.484193e-49 | 4.064611e-46 |
| MsG0480023136.01 | MsG0580026439.01 | -0.809297 | 2.028190e-50 | 1.186379e-47 |
| MsG0480023136.01 | MsG0580029283.01 | -0.810835 | 9.461727e-51 | 5.764269e-48 |
| MsG0480023136.01 | MsG0680031051.01 | -0.810453 | 1.144308e-50 | 6.900181e-48 |
| MsG0480023136.01 | MsG0680031446.01 | 0.808942 | 2.417319e-50 | 1.400507e-47 |
| MsG0480023136.01 | MsG0680031536.01 | -0.823647 | 1.237331e-53 | 1.071779e-50 |
| MsG0480023136.01 | MsG0680032510.01 | 0.859253 | 4.699710e-63 | 1.233235e-59 |
| MsG0480023136.01 | MsG0680035854.01 | -0.803642 | 3.162680e-49 | 1.597658e-46 |
| MsG0480023136.01 | MsG0780036247.01 | 0.850633 | 1.496367e-60 | 2.949895e-57 |
| MsG0480023136.01 | MsG0780036544.01 | 0.831462 | 1.646710e-55 | 1.788293e-52 |
| MsG0480023136.01 | MsG0780039340.01 | -0.813557 | 2.410176e-51 | 1.579043e-48 |
| MsG0480023136.01 | MsG0780040375.01 | 0.801885 | 7.291782e-49 | 3.522049e-46 |
| MsG0480023136.01 | MsG0780041729.01 | 0.812089 | 5.052248e-51 | 3.182156e-48 |
| MsG0480023136.01 | MsG0880042219.01 | 0.838617 | 2.585529e-57 | 3.482084e-54 |
| MsG0480023136.01 | MsG0880042399.01 | 0.806762 | 7.028987e-50 | 3.846819e-47 |
| MsG0480023136.01 | MsG0880044062.01 | 0.803613 | 3.206749e-49 | 1.618725e-46 |
| MsG0480023136.01 | MsG0880045480.01 | 0.813988 | 1.936465e-51 | 1.283553e-48 |
| MsG0480023136.01 | MsG0880045631.01 | -0.803290 | 3.741389e-49 | 1.872939e-46 |
| MsG0480023136.01 | MsG0880045705.01 | 0.844229 | 8.603115e-59 | 1.379867e-55 |
| MsG0480023136.01 | MsG0880046377.01 | -0.814056 | 1.871070e-51 | 1.242505e-48 |
| MsG0480023136.01 | MsG0880047116.01 | -0.802979 | 4.337562e-49 | 2.154153e-46 |
| MsG0380015598.01 | MsG0480023136.01 | 0.804215 | 2.404139e-49 | 1.232453e-46 |
| MsG0380015818.01 | MsG0480023136.01 | -0.820656 | 6.112906e-53 | 4.865718e-50 |
| MsG0380016117.01 | MsG0480023136.01 | -0.839723 | 1.336188e-57 | 1.861345e-54 |
| MsG0380016118.01 | MsG0480023136.01 | 0.816767 | 4.667510e-52 | 3.337195e-49 |
| MsG0380016823.01 | MsG0480023136.01 | 0.828750 | 7.555887e-55 | 7.578053e-52 |
| MsG0380017279.01 | MsG0480023136.01 | -0.817467 | 3.248521e-52 | 2.367761e-49 |
| MsG0380017476.01 | MsG0480023136.01 | -0.809981 | 1.446429e-50 | 8.615307e-48 |
| MsG0380017512.01 | MsG0480023136.01 | -0.843822 | 1.105953e-58 | 1.751348e-55 |
| MsG0380017914.01 | MsG0480023136.01 | 0.800052 | 1.726605e-48 | 7.961116e-46 |
| MsG0280006682.01 | MsG0480023136.01 | -0.805136 | 1.544518e-49 | 8.108178e-47 |
| MsG0280006892.01 | MsG0480023136.01 | -0.819301 | 1.248185e-52 | 9.567771e-50 |
| MsG0280009350.01 | MsG0480023136.01 | -0.805293 | 1.431627e-49 | 7.545830e-47 |
| MsG0280009441.01 | MsG0480023136.01 | -0.802537 | 5.351376e-49 | 2.628023e-46 |
| MsG0280009637.01 | MsG0480023136.01 | 0.817978 | 2.491619e-52 | 1.841498e-49 |
| MsG0280010423.01 | MsG0480023136.01 | 0.823186 | 1.586076e-53 | 1.355970e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023136.01.T01 | MTR_8g087820 | 87.302 | 63 | 8 | 0 | 1 | 63 | 119 | 181 | 2.04e-33 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023136.01.T01 | AT4G18930 | 62.069 | 58 | 22 | 0 | 1 | 58 | 117 | 174 | 2.00e-20 | 80.5 |
| MsG0480023136.01.T01 | AT4G18940 | 50.000 | 58 | 26 | 1 | 1 | 58 | 135 | 189 | 4.25e-13 | 61.6 |
Find 12 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGTGTTGGCAAGCTAGTT+TGG | 0.341344 | 4:-81270698 | None:intergenic |
| AAGACAAATCTCTTAAATCT+TGG | 0.349286 | 4:+81270649 | MsG0480023136.01.T01:CDS |
| AGACAAATCTCTTAAATCTT+GGG | 0.434674 | 4:+81270650 | MsG0480023136.01.T01:CDS |
| GAGTGATGAAGAGAAGCAAA+AGG | 0.529008 | 4:+81270539 | MsG0480023136.01.T01:CDS |
| CCACACGTGAGCCTTCTGTA+TGG | 0.533433 | 4:+81270510 | MsG0480023136.01.T01:CDS |
| GCTTACTTATCTGAAAGCTC+AGG | 0.559929 | 4:-81270602 | None:intergenic |
| CACTCAAATCTCCATACAGA+AGG | 0.585673 | 4:-81270521 | None:intergenic |
| TGAAGTTGTGATGGTATAAG+TGG | 0.589557 | 4:-81270717 | None:intergenic |
| TGTGATGGTATAAGTGGTGT+TGG | 0.591568 | 4:-81270711 | None:intergenic |
| CAACACTTGTGAAGTTGTGA+TGG | 0.638158 | 4:-81270726 | None:intergenic |
| CCATACAGAAGGCTCACGTG+TGG | 0.675755 | 4:-81270510 | None:intergenic |
| ATTCTTGACGACAGTCTCAG+TGG | 0.687938 | 4:+81270579 | MsG0480023136.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGACAAATCTCTTAAATCT+TGG | + | Chr4:81270649-81270668 | MsG0480023136.01.T01:CDS | 25.0% |
| ! | AGACAAATCTCTTAAATCTT+GGG | + | Chr4:81270650-81270669 | MsG0480023136.01.T01:CDS | 25.0% |
| TGAAGTTGTGATGGTATAAG+TGG | - | Chr4:81270720-81270739 | None:intergenic | 35.0% | |
| CAACACTTGTGAAGTTGTGA+TGG | - | Chr4:81270729-81270748 | None:intergenic | 40.0% | |
| CACTCAAATCTCCATACAGA+AGG | - | Chr4:81270524-81270543 | None:intergenic | 40.0% | |
| GAGTGATGAAGAGAAGCAAA+AGG | + | Chr4:81270539-81270558 | MsG0480023136.01.T01:CDS | 40.0% | |
| GCTTACTTATCTGAAAGCTC+AGG | - | Chr4:81270605-81270624 | None:intergenic | 40.0% | |
| TGTGATGGTATAAGTGGTGT+TGG | - | Chr4:81270714-81270733 | None:intergenic | 40.0% | |
| ATTCTTGACGACAGTCTCAG+TGG | + | Chr4:81270579-81270598 | MsG0480023136.01.T01:CDS | 45.0% | |
| !! | GTGGTGTTGGCAAGCTAGTT+TGG | - | Chr4:81270701-81270720 | None:intergenic | 50.0% |
| CCACACGTGAGCCTTCTGTA+TGG | + | Chr4:81270510-81270529 | MsG0480023136.01.T01:CDS | 55.0% | |
| CCATACAGAAGGCTCACGTG+TGG | - | Chr4:81270513-81270532 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 81270507 | 81270764 | 81270507 | ID=MsG0480023136.01;Name=MsG0480023136.01 |
| Chr4 | mRNA | 81270507 | 81270764 | 81270507 | ID=MsG0480023136.01.T01;Parent=MsG0480023136.01;Name=MsG0480023136.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|85 |
| Chr4 | exon | 81270507 | 81270764 | 81270507 | ID=MsG0480023136.01.T01:exon:669;Parent=MsG0480023136.01.T01 |
| Chr4 | CDS | 81270507 | 81270764 | 81270507 | ID=MsG0480023136.01.T01:cds;Parent=MsG0480023136.01.T01 |
| Gene Sequence |
| Protein sequence |
