AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036247.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036247.01.T01 MTR_5g008420 87.755 49 6 0 18 66 628 676 9.59e-20 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACCTGGTTGCCCATAAC+CGG 0.242390 7:+5219254 None:intergenic
CCTGGTGGACAACCCGGTTA+TGG 0.315095 7:-5219267 MsG0780036247.01.T01:CDS
CGCATAACTAGGTGCGGCCT+GGG 0.374054 7:+5219233 None:intergenic
CAAGCAGCATATCCTACATA+TGG 0.383624 7:-5219361 MsG0780036247.01.T01:CDS
CTGGTGGACAACCCGGTTAT+GGG 0.385117 7:-5219266 MsG0780036247.01.T01:CDS
GCGCATAACTAGGTGCGGCC+TGG 0.388775 7:+5219232 None:intergenic
TATGCGCAAGTTGGTCATAC+TGG 0.399918 7:-5219216 MsG0780036247.01.T01:CDS
AACCGGGTTGTCCACCAGGC+TGG 0.415446 7:+5219271 None:intergenic
CTATATCTATATGCATCATC+GGG 0.416909 7:-5219391 None:intergenic
CAAGTTGGTCATACTGGTTA+TGG 0.420943 7:-5219210 MsG0780036247.01.T01:CDS
CTAGGTGCGGCCTGGGCACC+TGG 0.421749 7:+5219240 None:intergenic
CCATAACCGGGTTGTCCACC+AGG 0.453267 7:+5219267 None:intergenic
ACTATATCTATATGCATCAT+CGG 0.493050 7:-5219392 None:intergenic
ACCGGGTTGTCCACCAGGCT+GGG 0.494134 7:+5219272 None:intergenic
GGCTGGGCATAAACTTGTCC+AGG 0.498525 7:+5219288 None:intergenic
TGACCAACTTGCGCATAACT+AGG 0.503715 7:+5219222 None:intergenic
AATCACCCGCAGTCTGCACC+TGG 0.524809 7:-5219306 MsG0780036247.01.T01:CDS
TTATGGGCAACCAGGTGCCC+AGG 0.525214 7:-5219250 MsG0780036247.01.T01:CDS
CTTGTCCAGGTGCAGACTGC+GGG 0.527053 7:+5219301 None:intergenic
GCCCAGCCTGGTGGACAACC+CGG 0.535827 7:-5219273 MsG0780036247.01.T01:CDS
GCACCTGGTTGCCCATAACC+GGG 0.540537 7:+5219255 None:intergenic
GGACAAGTTTATGCCCAGCC+TGG 0.546505 7:-5219285 MsG0780036247.01.T01:CDS
CACTCTGCACTAGCTGCCGA+TGG 0.553366 7:-5219333 MsG0780036247.01.T01:CDS
CAACCCGGTTATGGGCAACC+AGG 0.556358 7:-5219258 MsG0780036247.01.T01:CDS
AAGCAGCATATCCTACATAT+GGG 0.560036 7:-5219360 MsG0780036247.01.T01:CDS
CAGAGTGCAGACCCATATGT+AGG 0.574828 7:+5219349 None:intergenic
CAAGTTTATGCCCAGCCTGG+TGG 0.621083 7:-5219282 MsG0780036247.01.T01:CDS
CTGCGGGTGATTGTAACCAT+CGG 0.627649 7:+5219317 None:intergenic
GCACCTAGTTATGCGCAAGT+TGG 0.628557 7:-5219225 MsG0780036247.01.T01:CDS
ACTTGTCCAGGTGCAGACTG+CGG 0.644028 7:+5219300 None:intergenic
AACTTGCGCATAACTAGGTG+CGG 0.662539 7:+5219227 None:intergenic
TATATCTATATGCATCATCG+GGG 0.741642 7:-5219390 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCAGCATATCCTACATAT+GGG - Chr7:5219224-5219243 MsG0780036247.01.T01:CDS 35.0%
CAAGCAGCATATCCTACATA+TGG - Chr7:5219223-5219242 MsG0780036247.01.T01:CDS 40.0%
!! CAAGTTGGTCATACTGGTTA+TGG - Chr7:5219374-5219393 MsG0780036247.01.T01:CDS 40.0%
AACTTGCGCATAACTAGGTG+CGG + Chr7:5219360-5219379 None:intergenic 45.0%
TGACCAACTTGCGCATAACT+AGG + Chr7:5219365-5219384 None:intergenic 45.0%
!! TATGCGCAAGTTGGTCATAC+TGG - Chr7:5219368-5219387 MsG0780036247.01.T01:CDS 45.0%
CAGAGTGCAGACCCATATGT+AGG + Chr7:5219238-5219257 None:intergenic 50.0%
CTGCGGGTGATTGTAACCAT+CGG + Chr7:5219270-5219289 None:intergenic 50.0%
GCACCTAGTTATGCGCAAGT+TGG - Chr7:5219359-5219378 MsG0780036247.01.T01:CDS 50.0%
ACTTGTCCAGGTGCAGACTG+CGG + Chr7:5219287-5219306 None:intergenic 55.0%
CAAGTTTATGCCCAGCCTGG+TGG - Chr7:5219302-5219321 MsG0780036247.01.T01:CDS 55.0%
GGACAAGTTTATGCCCAGCC+TGG - Chr7:5219299-5219318 MsG0780036247.01.T01:CDS 55.0%
GGCTGGGCATAAACTTGTCC+AGG + Chr7:5219299-5219318 None:intergenic 55.0%
! CTGGTGGACAACCCGGTTAT+GGG - Chr7:5219318-5219337 MsG0780036247.01.T01:CDS 55.0%
AATCACCCGCAGTCTGCACC+TGG - Chr7:5219278-5219297 MsG0780036247.01.T01:CDS 60.0%
CAACCCGGTTATGGGCAACC+AGG - Chr7:5219326-5219345 MsG0780036247.01.T01:CDS 60.0%
CACTCTGCACTAGCTGCCGA+TGG - Chr7:5219251-5219270 MsG0780036247.01.T01:CDS 60.0%
CCATAACCGGGTTGTCCACC+AGG + Chr7:5219320-5219339 None:intergenic 60.0%
CGCATAACTAGGTGCGGCCT+GGG + Chr7:5219354-5219373 None:intergenic 60.0%
CTTGTCCAGGTGCAGACTGC+GGG + Chr7:5219286-5219305 None:intergenic 60.0%
GCACCTGGTTGCCCATAACC+GGG + Chr7:5219332-5219351 None:intergenic 60.0%
GGCACCTGGTTGCCCATAAC+CGG + Chr7:5219333-5219352 None:intergenic 60.0%
TTATGGGCAACCAGGTGCCC+AGG - Chr7:5219334-5219353 MsG0780036247.01.T01:CDS 60.0%
! CCTGGTGGACAACCCGGTTA+TGG - Chr7:5219317-5219336 MsG0780036247.01.T01:CDS 60.0%
AACCGGGTTGTCCACCAGGC+TGG + Chr7:5219316-5219335 None:intergenic 65.0%
ACCGGGTTGTCCACCAGGCT+GGG + Chr7:5219315-5219334 None:intergenic 65.0%
GCGCATAACTAGGTGCGGCC+TGG + Chr7:5219355-5219374 None:intergenic 65.0%
! GCCCAGCCTGGTGGACAACC+CGG - Chr7:5219311-5219330 MsG0780036247.01.T01:CDS 70.0%
CTAGGTGCGGCCTGGGCACC+TGG + Chr7:5219347-5219366 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 5219203 5219403 5219203 ID=MsG0780036247.01;Name=MsG0780036247.01
Chr7 mRNA 5219203 5219403 5219203 ID=MsG0780036247.01.T01;Parent=MsG0780036247.01;Name=MsG0780036247.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|66
Chr7 exon 5219203 5219403 5219203 ID=MsG0780036247.01.T01:exon:2874;Parent=MsG0780036247.01.T01
Chr7 CDS 5219203 5219403 5219203 ID=MsG0780036247.01.T01:cds;Parent=MsG0780036247.01.T01
Gene Sequence

>MsG0780036247.01.T01

ATGCATCATCGGGGTCATCGCAAGCAGCATATCCTACATATGGGTCTGCACTCTGCACTAGCTGCCGATGGTTACAATCACCCGCAGTCTGCACCTGGACAAGTTTATGCCCAGCCTGGTGGACAACCCGGTTATGGGCAACCAGGTGCCCAGGCCGCACCTAGTTATGCGCAAGTTGGTCATACTGGTTATGGATCATAA

Protein sequence

>MsG0780036247.01.T01

MHHRGHRKQHILHMGLHSALAADGYNHPQSAPGQVYAQPGGQPGYGQPGAQAAPSYAQVGHTGYGS*