AlfalfaGEDB | MsG0480023841.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023841.01

KEGG: K01754
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000400.01	MsG0480023841.01	0.849521	3.065132e-60	5.826094e-57
MsG0180001315.01	MsG0480023841.01	-0.800214	1.601054e-48	7.412636e-46
MsG0180003181.01	MsG0480023841.01	-0.800197	1.613393e-48	7.466385e-46
MsG0180003303.01	MsG0480023841.01	-0.801706	7.933627e-49	3.814457e-46
MsG0180003379.01	MsG0480023841.01	-0.854951	8.742090e-62	1.985751e-58
MsG0180004016.01	MsG0480023841.01	-0.830298	3.177021e-55	3.333569e-52
MsG0180004684.01	MsG0480023841.01	-0.808831	2.552190e-50	1.474327e-47
MsG0180005049.01	MsG0480023841.01	-0.800494	1.403797e-48	6.545767e-46
MsG0180005106.01	MsG0480023841.01	-0.807618	4.629104e-50	2.590352e-47
MsG0180006025.01	MsG0480023841.01	-0.800871	1.175786e-48	5.535420e-46
MsG0280006368.01	MsG0480023841.01	-0.807703	4.439533e-50	2.489827e-47
MsG0280011075.01	MsG0480023841.01	-0.802945	4.409116e-49	2.187812e-46
MsG0280011287.01	MsG0480023841.01	-0.825180	5.396093e-54	4.882746e-51
MsG0480019984.01	MsG0480023841.01	0.827408	1.590752e-54	1.534528e-51
MsG0480021432.01	MsG0480023841.01	-0.813270	2.786056e-51	1.811039e-48
MsG0480021876.01	MsG0480023841.01	0.863479	2.418196e-64	7.340467e-61
MsG0480021986.01	MsG0480023841.01	-0.805767	1.138926e-49	6.076867e-47
MsG0480023136.01	MsG0480023841.01	0.806195	9.256263e-50	4.993102e-47
MsG0480023240.01	MsG0480023841.01	-0.817656	2.945140e-52	2.157627e-49
MsG0480023840.01	MsG0480023841.01	0.827922	1.197257e-54	1.172213e-51
MsG0480023841.01	MsG0580024581.01	-0.800303	1.535546e-48	7.125685e-46
MsG0480023841.01	MsG0580028664.01	0.835244	1.878287e-56	2.283758e-53
MsG0480023841.01	MsG0580029628.01	-0.811297	7.512178e-51	4.632632e-48
MsG0480023841.01	MsG0580030003.01	-0.802170	6.371533e-49	3.099805e-46
MsG0480023841.01	MsG0680031536.01	-0.816786	4.621730e-52	3.306257e-49
MsG0480023841.01	MsG0680031597.01	0.812830	3.480627e-51	2.235788e-48
MsG0480023841.01	MsG0680032510.01	0.800492	1.405192e-48	6.551908e-46
MsG0480023841.01	MsG0780036247.01	0.838205	3.301929e-57	4.391055e-54
MsG0480023841.01	MsG0780036544.01	0.817393	3.376138e-52	2.455677e-49
MsG0480023841.01	MsG0780036845.01	-0.839534	1.496233e-57	2.072228e-54
MsG0480023841.01	MsG0780040375.01	0.801770	7.697806e-49	3.707040e-46
MsG0480023841.01	MsG0780040424.01	-0.827966	1.168373e-54	1.145500e-51
MsG0480023841.01	MsG0780040742.01	-0.805355	1.389634e-49	7.336280e-47
MsG0480023841.01	MsG0780041482.01	-0.818472	1.925716e-52	1.442901e-49
MsG0480023841.01	MsG0880042206.01	-0.807257	5.521705e-50	3.061086e-47
MsG0480023841.01	MsG0880042399.01	0.894683	1.958097e-75	1.967089e-71
MsG0480023841.01	MsG0880043162.01	-0.820393	7.025415e-53	5.552246e-50
MsG0480023841.01	MsG0880043194.01	-0.822461	2.340270e-53	1.960069e-50
MsG0480023841.01	MsG0880045165.01	-0.817872	2.631603e-52	1.939437e-49
MsG0480023841.01	MsG0880045408.01	-0.806556	7.767054e-50	4.228529e-47
MsG0480023841.01	MsG0880045480.01	0.861705	8.500952e-64	2.426413e-60
MsG0480023841.01	MsG0880045705.01	0.840098	1.066768e-57	1.503757e-54
MsG0480023841.01	MsG0880046509.01	0.806615	7.546509e-50	4.114523e-47
MsG0480023841.01	MsG0880046684.01	-0.807620	4.623407e-50	2.587355e-47
MsG0480023841.01	MsG0880047328.01	0.851684	7.560888e-61	1.542476e-57
MsG0380015598.01	MsG0480023841.01	0.822767	1.986496e-53	1.678427e-50
MsG0380015607.01	MsG0480023841.01	-0.849758	2.632435e-60	5.042624e-57
MsG0380016117.01	MsG0480023841.01	-0.858319	8.941024e-63	2.272843e-59
MsG0380016178.01	MsG0480023841.01	0.836049	1.175023e-56	1.463547e-53
MsG0380017210.01	MsG0480023841.01	-0.802615	5.157034e-49	2.537618e-46
MsG0380017229.01	MsG0480023841.01	-0.835622	1.507684e-56	1.853758e-53
MsG0380017476.01	MsG0480023841.01	-0.815239	1.023646e-51	7.018578e-49
MsG0380017797.01	MsG0480023841.01	-0.809857	1.538142e-50	9.131591e-48
MsG0480018292.01	MsG0480023841.01	-0.848765	4.976203e-60	9.229567e-57
MsG0280006519.01	MsG0480023841.01	-0.827508	1.505520e-54	1.456369e-51
MsG0280006682.01	MsG0480023841.01	-0.821903	3.152650e-53	2.598808e-50
MsG0280007011.01	MsG0480023841.01	-0.829393	5.279660e-55	5.396739e-52
MsG0280007775.01	MsG0480023841.01	-0.815046	1.129867e-51	7.706542e-49
MsG0280009554.01	MsG0480023841.01	-0.814424	1.551780e-51	1.040867e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 48 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAGTCTTGTTTCAGAAAA+TGG	0.136929	4:+90391629	None:intergenic
TGCTTTGCCGACGTTATTAC+AGG	0.301052	4:-90391402	MsG0480023841.01.T01:CDS
TATATCTATTGTTCGACCTT+CGG	0.358406	4:-90391662	MsG0480023841.01.T01:CDS
GCAGTCTTGTTTCAGAAAAT+GGG	0.359660	4:+90391630	None:intergenic
CACTATCACTTCAACACCTT+TGG	0.378364	4:+90391532	None:intergenic
CACCATCACTTCAACACCTT+TGG	0.384125	4:+90391499	None:intergenic
TTTCAGTGCGATTGATGATT+GGG	0.398288	4:-90391337	MsG0480023841.01.T01:CDS
CGCCATCACTTCTACGCCTT+CGG	0.418570	4:+90391565	None:intergenic
CGTCACTTCAACAACACCTT+TGG	0.427483	4:+90391601	None:intergenic
TGTATGTGTGTTCTCTGTAA+TGG	0.430646	4:+90391743	None:intergenic
AATAACGTCGGCAAAGCAAT+TGG	0.454926	4:+90391407	None:intergenic
GGCAGCGCAACAGCAAGGAC+TGG	0.462015	4:+90391373	None:intergenic
CTGTTGCGCTGCCTCAGTAC+TGG	0.464401	4:-90391363	MsG0480023841.01.T01:CDS
GGTCGAACAATAGATATAGA+AGG	0.470732	4:+90391667	None:intergenic
AGATGAGCAGAGGTACCGTT+TGG	0.479667	4:+90391700	None:intergenic
GCAGCGCAACAGCAAGGACT+GGG	0.486221	4:+90391374	None:intergenic
CACCATCACTTCAACACCTA+TGG	0.496104	4:+90391433	None:intergenic
CACCATCACTTCAACACCTA+TGG	0.496104	4:+90391466	None:intergenic
CAGTCTTGTTTCAGAAAATG+GGG	0.524569	4:+90391631	None:intergenic
AAGTGAGTTAGAGACTCAAA+AGG	0.529599	4:+90391313	None:intergenic
GACCGAAGGCGTAGAAGTGA+TGG	0.554429	4:-90391567	MsG0480023841.01.T01:CDS
GTAGAAGTGATGGCGTTGCA+AGG	0.574567	4:-90391557	MsG0480023841.01.T01:CDS
GTTGAAGTGACGGTGTTGCA+AGG	0.590921	4:-90391590	MsG0480023841.01.T01:CDS
TTGAAGTGATAGTGTTGCAT+GGG	0.594681	4:-90391523	MsG0480023841.01.T01:CDS
ACAAGACTGCAAGGGACCAA+AGG	0.597556	4:-90391617	MsG0480023841.01.T01:CDS
ACGGTGTTGCAAGGGACCGA+AGG	0.599968	4:-90391581	MsG0480023841.01.T01:CDS
GACCATAGGTGTTGAAGTGA+TGG	0.600889	4:-90391435	MsG0480023841.01.T01:CDS
GACCATAGGTGTTGAAGTGA+TGG	0.600889	4:-90391468	MsG0480023841.01.T01:CDS
ATGGTGTTGCAAGGGACCAT+AGG	0.603345	4:-90391449	MsG0480023841.01.T01:CDS
ATGGTGTTGCAAGGGACCAT+AGG	0.603345	4:-90391482	MsG0480023841.01.T01:CDS
TAGAAGTGATGGCGTTGCAA+GGG	0.606725	4:-90391556	MsG0480023841.01.T01:CDS
ATGGCGTTGCAAGGGACCAA+AGG	0.607985	4:-90391548	MsG0480023841.01.T01:CDS
TTTGCCGACGTTATTACAGG+TGG	0.617033	4:-90391399	MsG0480023841.01.T01:CDS
TTGAAGTGACGGTGTTGCAA+GGG	0.618089	4:-90391589	MsG0480023841.01.T01:CDS
AAATGGGGAAATTAAGCCGA+AGG	0.620465	4:+90391646	None:intergenic
GACCAAAGGTGTTGAAGTGA+TGG	0.620830	4:-90391501	MsG0480023841.01.T01:CDS
GTTGAAGTGATGGTGTTGCA+AGG	0.625625	4:-90391458	MsG0480023841.01.T01:CDS
GTTGAAGTGATGGTGTTGCA+AGG	0.625625	4:-90391491	MsG0480023841.01.T01:CDS
AGTCTAACTTAGATGAGCAG+AGG	0.629549	4:+90391690	None:intergenic
TTGAAGTGATGGTGTTGCAA+GGG	0.633210	4:-90391457	MsG0480023841.01.T01:CDS
TTGAAGTGATGGTGTTGCAA+GGG	0.633210	4:-90391490	MsG0480023841.01.T01:CDS
ACTGAGGCAGCGCAACAGCA+AGG	0.636738	4:+90391368	None:intergenic
CAAAGGTGTTGTTGAAGTGA+CGG	0.642763	4:-90391600	MsG0480023841.01.T01:CDS
ATAGTGTTGCATGGGACCAA+AGG	0.652692	4:-90391515	MsG0480023841.01.T01:CDS
GTTGAAGTGATAGTGTTGCA+TGG	0.654500	4:-90391524	MsG0480023841.01.T01:CDS
TTTCTGAAACAAGACTGCAA+GGG	0.692177	4:-90391625	MsG0480023841.01.T01:CDS
GGATCCACCTGTAATAACGT+CGG	0.698709	4:+90391395	None:intergenic
CACTGAAAATACCAGTACTG+AGG	0.701584	4:+90391352	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATATCTATTGTTCGACCTT+CGG	-	Chr4:90391430-90391449	MsG0480023841.01.T01:CDS	30.0%
!!!	CTTCTAGTTTTACTTCCAAA+CGG	-	Chr4:90391377-90391396	MsG0480023841.01.T01:CDS	30.0%
	GGTCGAACAATAGATATAGA+AGG	+	Chr4:90391428-90391447	None:intergenic	35.0%
	TGTATGTGTGTTCTCTGTAA+TGG	+	Chr4:90391352-90391371	None:intergenic	35.0%
!	TTGAAGTGATAGTGTTGCAT+GGG	-	Chr4:90391569-90391588	MsG0480023841.01.T01:CDS	35.0%
!	TTTCAGTGCGATTGATGATT+GGG	-	Chr4:90391755-90391774	MsG0480023841.01.T01:CDS	35.0%
!!	CAGTCTTGTTTCAGAAAATG+GGG	+	Chr4:90391464-90391483	None:intergenic	35.0%
!!	GCAGTCTTGTTTCAGAAAAT+GGG	+	Chr4:90391465-90391484	None:intergenic	35.0%
!!	TGCAGTCTTGTTTCAGAAAA+TGG	+	Chr4:90391466-90391485	None:intergenic	35.0%
!!	TTTCTGAAACAAGACTGCAA+GGG	-	Chr4:90391467-90391486	MsG0480023841.01.T01:CDS	35.0%
!!	TTTTCAGTGCGATTGATGAT+TGG	-	Chr4:90391754-90391773	MsG0480023841.01.T01:CDS	35.0%
!!!	TTTTCTGAAACAAGACTGCA+AGG	-	Chr4:90391466-90391485	MsG0480023841.01.T01:CDS	35.0%
	AAATGGGGAAATTAAGCCGA+AGG	+	Chr4:90391449-90391468	None:intergenic	40.0%
	AATAACGTCGGCAAAGCAAT+TGG	+	Chr4:90391688-90391707	None:intergenic	40.0%
	AGTCTAACTTAGATGAGCAG+AGG	+	Chr4:90391405-90391424	None:intergenic	40.0%
	CACTATCACTTCAACACCTT+TGG	+	Chr4:90391563-90391582	None:intergenic	40.0%
	CACTGAAAATACCAGTACTG+AGG	+	Chr4:90391743-90391762	None:intergenic	40.0%
!	GTTGAAGTGATAGTGTTGCA+TGG	-	Chr4:90391568-90391587	MsG0480023841.01.T01:CDS	40.0%
!!	CAAAGGTGTTGTTGAAGTGA+CGG	-	Chr4:90391492-90391511	MsG0480023841.01.T01:CDS	40.0%
!!	TTGAAGTGATGGTGTTGCAA+GGG	-	Chr4:90391602-90391621	MsG0480023841.01.T01:CDS	40.0%
	ATAGTGTTGCATGGGACCAA+AGG	-	Chr4:90391577-90391596	MsG0480023841.01.T01:CDS	45.0%
	CACCATCACTTCAACACCTA+TGG	+	Chr4:90391629-90391648	None:intergenic	45.0%
	CACCATCACTTCAACACCTT+TGG	+	Chr4:90391596-90391615	None:intergenic	45.0%
	CGTCACTTCAACAACACCTT+TGG	+	Chr4:90391494-90391513	None:intergenic	45.0%
	GGATCCACCTGTAATAACGT+CGG	+	Chr4:90391700-90391719	None:intergenic	45.0%
	TGCTTTGCCGACGTTATTAC+AGG	-	Chr4:90391690-90391709	MsG0480023841.01.T01:CDS	45.0%
	TTTGCCGACGTTATTACAGG+TGG	-	Chr4:90391693-90391712	MsG0480023841.01.T01:CDS	45.0%
!	TAGAAGTGATGGCGTTGCAA+GGG	-	Chr4:90391536-90391555	MsG0480023841.01.T01:CDS	45.0%
!!	GACCAAAGGTGTTGAAGTGA+TGG	-	Chr4:90391558-90391577	MsG0480023841.01.T01:CDS	45.0%
!!	GACCATAGGTGTTGAAGTGA+TGG	-	Chr4:90391624-90391643	MsG0480023841.01.T01:CDS	45.0%
!!	GTTGAAGTGATGGTGTTGCA+AGG	-	Chr4:90391601-90391620	MsG0480023841.01.T01:CDS	45.0%
!!	TTGAAGTGACGGTGTTGCAA+GGG	-	Chr4:90391503-90391522	MsG0480023841.01.T01:CDS	45.0%
	ACAAGACTGCAAGGGACCAA+AGG	-	Chr4:90391475-90391494	MsG0480023841.01.T01:CDS	50.0%
!	GTAGAAGTGATGGCGTTGCA+AGG	-	Chr4:90391535-90391554	MsG0480023841.01.T01:CDS	50.0%
!!	AGATGAGCAGAGGTACCGTT+TGG	+	Chr4:90391395-90391414	None:intergenic	50.0%
!!	ATGGTGTTGCAAGGGACCAT+AGG	-	Chr4:90391610-90391629	MsG0480023841.01.T01:CDS	50.0%
!!	GTTGAAGTGACGGTGTTGCA+AGG	-	Chr4:90391502-90391521	MsG0480023841.01.T01:CDS	50.0%
	ATGGCGTTGCAAGGGACCAA+AGG	-	Chr4:90391544-90391563	MsG0480023841.01.T01:CDS	55.0%
	CGCCATCACTTCTACGCCTT+CGG	+	Chr4:90391530-90391549	None:intergenic	55.0%
!	GACCGAAGGCGTAGAAGTGA+TGG	-	Chr4:90391525-90391544	MsG0480023841.01.T01:CDS	55.0%
	ACTGAGGCAGCGCAACAGCA+AGG	+	Chr4:90391727-90391746	None:intergenic	60.0%
	CTGTTGCGCTGCCTCAGTAC+TGG	-	Chr4:90391729-90391748	MsG0480023841.01.T01:CDS	60.0%
	GCAGCGCAACAGCAAGGACT+GGG	+	Chr4:90391721-90391740	None:intergenic	60.0%
!!	ACGGTGTTGCAAGGGACCGA+AGG	-	Chr4:90391511-90391530	MsG0480023841.01.T01:CDS	60.0%
	GGCAGCGCAACAGCAAGGAC+TGG	+	Chr4:90391722-90391741	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	90391328	90391786	90391328	ID=MsG0480023841.01;Name=MsG0480023841.01
Chr4	mRNA	90391328	90391786	90391328	ID=MsG0480023841.01.T01;Parent=MsG0480023841.01;Name=MsG0480023841.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|152
Chr4	exon	90391328	90391786	90391328	ID=MsG0480023841.01.T01:exon:7593;Parent=MsG0480023841.01.T01
Chr4	CDS	90391328	90391786	90391328	ID=MsG0480023841.01.T01:cds;Parent=MsG0480023841.01.T01

Gene Sequence

>MsG0480023841.01.T01

ATGAAGAATGACAATGTTTTTCCATTACAGAGAACACACATACAAAATTCTTCTAGTTTTACTTCCAAACGGTACCTCTGCTCATCTAAGTTAGACTCCTTCTATATCTATTGTTCGACCTTCGGCTTAATTTCCCCATTTTCTGAAACAAGACTGCAAGGGACCAAAGGTGTTGTTGAAGTGACGGTGTTGCAAGGGACCGAAGGCGTAGAAGTGATGGCGTTGCAAGGGACCAAAGGTGTTGAAGTGATAGTGTTGCATGGGACCAAAGGTGTTGAAGTGATGGTGTTGCAAGGGACCATAGGTGTTGAAGTGATGGTGTTGCAAGGGACCATAGGTGTTGAAGTGATGGTGTTGCCAATTGCTTTGCCGACGTTATTACAGGTGGATCCCAGTCCTTGCTGTTGCGCTGCCTCAGTACTGGTATTTTCAGTGCGATTGATGATTGGGTCCTTTTGA

Protein sequence

>MsG0480023841.01.T01

MKNDNVFPLQRTHIQNSSSFTSKRYLCSSKLDSFYIYCSTFGLISPFSETRLQGTKGVVEVTVLQGTEGVEVMALQGTKGVEVIVLHGTKGVEVMVLQGTIGVEVMVLQGTIGVEVMVLPIALPTLLQVDPSPCCCAASVLVFSVRLMIGSF*