Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017914.01.T01 | XP_013462246.1 | 98.131 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 3.99E-69 | 212 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017914.01.T01 | A0A072V3Q4 | 98.131 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 1.91e-69 | 212 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048809.01 | MsG0380017914.01 | 0.803144 | 4.009843e-49 | 1.999844e-46 |
| MsG0080048982.01 | MsG0380017914.01 | -0.806406 | 8.353194e-50 | 4.530119e-47 |
| MsG0180000680.01 | MsG0380017914.01 | -0.812997 | 3.198556e-51 | 2.063930e-48 |
| MsG0180002140.01 | MsG0380017914.01 | -0.826788 | 2.238126e-54 | 2.120004e-51 |
| MsG0180002185.01 | MsG0380017914.01 | -0.805451 | 1.326533e-49 | 7.020462e-47 |
| MsG0180003118.01 | MsG0380017914.01 | 0.829924 | 3.920091e-55 | 4.069433e-52 |
| MsG0180003379.01 | MsG0380017914.01 | -0.832257 | 1.048232e-55 | 1.165212e-52 |
| MsG0180004293.01 | MsG0380017914.01 | 0.800031 | 1.743993e-48 | 8.036930e-46 |
| MsG0180004385.01 | MsG0380017914.01 | -0.802313 | 5.952408e-49 | 2.906701e-46 |
| MsG0180004684.01 | MsG0380017914.01 | -0.811564 | 6.573611e-51 | 4.082691e-48 |
| MsG0180005106.01 | MsG0380017914.01 | -0.847067 | 1.462145e-59 | 2.568220e-56 |
| MsG0180005160.01 | MsG0380017914.01 | -0.808492 | 3.015953e-50 | 1.726908e-47 |
| MsG0180005354.01 | MsG0380017914.01 | -0.838570 | 2.658172e-57 | 3.574735e-54 |
| MsG0180006050.01 | MsG0380017914.01 | -0.812183 | 4.818184e-51 | 3.042528e-48 |
| MsG0280010976.01 | MsG0380017914.01 | -0.826793 | 2.231934e-54 | 2.114440e-51 |
| MsG0280011287.01 | MsG0380017914.01 | -0.840268 | 9.632265e-58 | 1.364785e-54 |
| MsG0380011895.01 | MsG0380017914.01 | -0.805816 | 1.112183e-49 | 5.941530e-47 |
| MsG0380012161.01 | MsG0380017914.01 | -0.807275 | 5.472389e-50 | 3.035225e-47 |
| MsG0380012458.01 | MsG0380017914.01 | -0.807704 | 4.439226e-50 | 2.489668e-47 |
| MsG0380013471.01 | MsG0380017914.01 | -0.820078 | 8.295067e-53 | 6.498850e-50 |
| MsG0380014551.01 | MsG0380017914.01 | 0.822783 | 1.969137e-53 | 1.664457e-50 |
| MsG0380014866.01 | MsG0380017914.01 | -0.819493 | 1.128115e-52 | 8.694913e-50 |
| MsG0380015215.01 | MsG0380017914.01 | 0.841419 | 4.806054e-58 | 7.058179e-55 |
| MsG0380015614.01 | MsG0380017914.01 | -0.813093 | 3.047642e-51 | 1.971498e-48 |
| MsG0380016117.01 | MsG0380017914.01 | -0.821145 | 4.718561e-53 | 3.807649e-50 |
| MsG0380016178.01 | MsG0380017914.01 | 0.808095 | 3.664372e-50 | 2.076413e-47 |
| MsG0380016223.01 | MsG0380017914.01 | -0.804674 | 1.928915e-49 | 1.000623e-46 |
| MsG0380016740.01 | MsG0380017914.01 | -0.808496 | 3.010358e-50 | 1.723870e-47 |
| MsG0380017229.01 | MsG0380017914.01 | -0.840739 | 7.252594e-58 | 1.042786e-54 |
| MsG0380017265.01 | MsG0380017914.01 | 0.823293 | 1.497366e-53 | 1.283968e-50 |
| MsG0380017401.01 | MsG0380017914.01 | -0.809285 | 2.040237e-50 | 1.193037e-47 |
| MsG0380017512.01 | MsG0380017914.01 | -0.810899 | 9.163624e-51 | 5.592366e-48 |
| MsG0380017914.01 | MsG0480018192.01 | 0.806487 | 8.032561e-50 | 4.365287e-47 |
| MsG0380017914.01 | MsG0480018244.01 | 0.808597 | 2.864883e-50 | 1.644813e-47 |
| MsG0380017914.01 | MsG0480018847.01 | -0.827770 | 1.302336e-54 | 1.269420e-51 |
| MsG0380017914.01 | MsG0480019289.01 | -0.804103 | 2.536768e-49 | 1.296764e-46 |
| MsG0380017914.01 | MsG0480020327.01 | 0.819816 | 9.522250e-53 | 7.405575e-50 |
| MsG0380017914.01 | MsG0480020719.01 | -0.818290 | 2.117410e-52 | 1.578455e-49 |
| MsG0380017914.01 | MsG0480022248.01 | -0.844805 | 6.022630e-59 | 9.836511e-56 |
| MsG0380017914.01 | MsG0480022392.01 | 0.821906 | 3.146306e-53 | 2.593849e-50 |
| MsG0380017914.01 | MsG0480022393.01 | 0.820202 | 7.767231e-53 | 6.105929e-50 |
| MsG0380017914.01 | MsG0480022933.01 | -0.809963 | 1.459128e-50 | 8.686951e-48 |
| MsG0380017914.01 | MsG0480023136.01 | 0.800052 | 1.726605e-48 | 7.961116e-46 |
| MsG0380017914.01 | MsG0480023377.01 | -0.801555 | 8.520592e-49 | 4.081059e-46 |
| MsG0380017914.01 | MsG0580024581.01 | -0.853871 | 1.793668e-61 | 3.932319e-58 |
| MsG0380017914.01 | MsG0580024650.01 | 0.803976 | 2.696123e-49 | 1.373762e-46 |
| MsG0380017914.01 | MsG0580025278.01 | -0.811471 | 6.887228e-51 | 4.267022e-48 |
| MsG0380017914.01 | MsG0580025520.01 | -0.803293 | 3.735351e-49 | 1.870087e-46 |
| MsG0380017914.01 | MsG0580028459.01 | -0.804166 | 2.461435e-49 | 1.260256e-46 |
| MsG0380017914.01 | MsG0580028526.01 | -0.815628 | 8.386249e-52 | 5.812477e-49 |
| MsG0380017914.01 | MsG0580029208.01 | -0.804089 | 2.554032e-49 | 1.305114e-46 |
| MsG0380017914.01 | MsG0580029628.01 | -0.819717 | 1.003248e-52 | 7.780732e-50 |
| MsG0380017914.01 | MsG0580029639.01 | -0.818327 | 2.076851e-52 | 1.549844e-49 |
| MsG0380017914.01 | MsG0680031377.01 | 0.805122 | 1.554864e-49 | 8.159553e-47 |
| MsG0380017914.01 | MsG0680031536.01 | -0.801437 | 9.008399e-49 | 4.301974e-46 |
| MsG0380017914.01 | MsG0680034235.01 | -0.823675 | 1.219233e-53 | 1.056956e-50 |
| MsG0380017914.01 | MsG0780036117.01 | -0.823096 | 1.665137e-53 | 1.420022e-50 |
| MsG0380017914.01 | MsG0780036247.01 | 0.818026 | 2.429440e-52 | 1.797944e-49 |
| MsG0380017914.01 | MsG0780036845.01 | -0.809021 | 2.324611e-50 | 1.349671e-47 |
| MsG0380017914.01 | MsG0780037330.01 | -0.801894 | 7.260631e-49 | 3.507766e-46 |
| MsG0380017914.01 | MsG0780038092.01 | -0.805304 | 1.423821e-49 | 7.506811e-47 |
| MsG0380017914.01 | MsG0780038644.01 | -0.813239 | 2.830761e-51 | 1.838459e-48 |
| MsG0380017914.01 | MsG0780038833.01 | -0.809403 | 1.925498e-50 | 1.129483e-47 |
| MsG0380017914.01 | MsG0780040375.01 | 0.845863 | 3.114595e-59 | 5.262773e-56 |
| MsG0380017914.01 | MsG0780040424.01 | -0.821473 | 3.962611e-53 | 3.226898e-50 |
| MsG0380017914.01 | MsG0780041557.01 | 0.801759 | 7.737776e-49 | 3.725204e-46 |
| MsG0380017914.01 | MsG0780041729.01 | 0.801545 | 8.561214e-49 | 4.099500e-46 |
| MsG0380017914.01 | MsG0880042743.01 | -0.828783 | 7.419327e-55 | 7.448389e-52 |
| MsG0380017914.01 | MsG0880043162.01 | -0.808112 | 3.635073e-50 | 2.060647e-47 |
| MsG0380017914.01 | MsG0880044409.01 | -0.814550 | 1.455187e-51 | 9.793968e-49 |
| MsG0380017914.01 | MsG0880045756.01 | -0.800742 | 1.249454e-48 | 5.862803e-46 |
| MsG0380017914.01 | MsG0880046404.01 | -0.802390 | 5.739449e-49 | 2.808167e-46 |
| MsG0280006462.01 | MsG0380017914.01 | -0.804856 | 1.767100e-49 | 9.210609e-47 |
| MsG0280006935.01 | MsG0380017914.01 | -0.811740 | 6.017662e-51 | 3.754825e-48 |
| MsG0280007109.01 | MsG0380017914.01 | -0.803181 | 3.940670e-49 | 1.967240e-46 |
| MsG0280007691.01 | MsG0380017914.01 | -0.800367 | 1.489822e-48 | 6.924739e-46 |
| MsG0280007775.01 | MsG0380017914.01 | -0.844204 | 8.740107e-59 | 1.400778e-55 |
| MsG0280009270.01 | MsG0380017914.01 | -0.802241 | 6.158201e-49 | 3.001535e-46 |
| MsG0280009554.01 | MsG0380017914.01 | -0.805619 | 1.223168e-49 | 6.501256e-47 |
| MsG0280009676.01 | MsG0380017914.01 | 0.819209 | 1.309840e-52 | 1.001546e-49 |
| MsG0280010158.01 | MsG0380017914.01 | -0.809608 | 1.739713e-50 | 1.026128e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017914.01.T01 | MTR_3g115060 | 98.131 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 4.84e-73 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017914.01.T01 | AT1G20430 | 65.882 | 85 | 29 | 0 | 23 | 107 | 29 | 113 | 1.09e-33 | 113 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTAAGCATGAAATCTT+TGG | 0.222509 | 3:-102145726 | MsG0380017914.01.T01:CDS |
| CTCTTAAGCATGAAATCTTT+GGG | 0.311239 | 3:-102145725 | MsG0380017914.01.T01:CDS |
| CCGAATGCCGCCGGTGCCGT+TGG | 0.453325 | 3:-102145833 | MsG0380017914.01.T01:CDS |
| CCTCTTGGAGTCCATGGATT+CGG | 0.472053 | 3:+102145693 | None:intergenic |
| CCAACGGCACCGGCGGCATT+CGG | 0.480360 | 3:+102145833 | None:intergenic |
| TCAGATCGTGAGTATCCTCT+TGG | 0.496938 | 3:+102145678 | None:intergenic |
| ATTCGGTCGCACTGAGGAGG+AGG | 0.524049 | 3:+102145850 | None:intergenic |
| GAAGCTTCTCAAGATTCTAC+TGG | 0.533479 | 3:-102145596 | MsG0380017914.01.T01:CDS |
| GGACAAAAGTACCGAATCCA+TGG | 0.544794 | 3:-102145704 | MsG0380017914.01.T01:CDS |
| AAGAGGATACTCACGATCTG+AGG | 0.545920 | 3:-102145676 | MsG0380017914.01.T01:CDS |
| GAGTATCCTCTTGGAGTCCA+TGG | 0.546526 | 3:+102145687 | None:intergenic |
| CTTCTTCGAACCAACGGCAC+CGG | 0.551741 | 3:+102145823 | None:intergenic |
| TAGCTTCTTCTTCGAACCAA+CGG | 0.568765 | 3:+102145817 | None:intergenic |
| CTTCGAACCAACGGCACCGG+CGG | 0.570741 | 3:+102145826 | None:intergenic |
| GGCATTCGGTCGCACTGAGG+AGG | 0.571642 | 3:+102145847 | None:intergenic |
| CACTGAGGAGGAGGAAGACA+TGG | 0.572521 | 3:+102145859 | None:intergenic |
| TCAGTGCGACCGAATGCCGC+CGG | 0.579043 | 3:-102145842 | MsG0380017914.01.T01:CDS |
| AGAGGATACTCACGATCTGA+GGG | 0.605728 | 3:-102145675 | MsG0380017914.01.T01:CDS |
| CCGAATCCATGGACTCCAAG+AGG | 0.665176 | 3:-102145693 | MsG0380017914.01.T01:CDS |
| GGCGGCATTCGGTCGCACTG+AGG | 0.704327 | 3:+102145844 | None:intergenic |
| ATTCATTGAAGAGACTACGA+AGG | 0.718138 | 3:+102145558 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTTAAGCATGAAATCTTT+GGG | - | Chr3:102145688-102145707 | MsG0380017914.01.T01:CDS | 30.0% | |
| TCTCTTAAGCATGAAATCTT+TGG | - | Chr3:102145687-102145706 | MsG0380017914.01.T01:CDS | 30.0% | |
| ! | CTTGATGTTTTTCATACGAT+CGG | + | Chr3:102145782-102145801 | None:intergenic | 30.0% |
| ATTCATTGAAGAGACTACGA+AGG | + | Chr3:102145858-102145877 | None:intergenic | 35.0% | |
| ! | ATTCAACTTTTCGCAATGAC+TGG | - | Chr3:102145658-102145677 | MsG0380017914.01.T01:CDS | 35.0% |
| GAAGCTTCTCAAGATTCTAC+TGG | - | Chr3:102145817-102145836 | MsG0380017914.01.T01:CDS | 40.0% | |
| TAGCTTCTTCTTCGAACCAA+CGG | + | Chr3:102145599-102145618 | None:intergenic | 40.0% | |
| AAGAGGATACTCACGATCTG+AGG | - | Chr3:102145737-102145756 | MsG0380017914.01.T01:CDS | 45.0% | |
| AGAGGATACTCACGATCTGA+GGG | - | Chr3:102145738-102145757 | MsG0380017914.01.T01:CDS | 45.0% | |
| TCAGATCGTGAGTATCCTCT+TGG | + | Chr3:102145738-102145757 | None:intergenic | 45.0% | |
| !! | GGACAAAAGTACCGAATCCA+TGG | - | Chr3:102145709-102145728 | MsG0380017914.01.T01:CDS | 45.0% |
| CCTCTTGGAGTCCATGGATT+CGG | + | Chr3:102145723-102145742 | None:intergenic | 50.0% | |
| GAGTATCCTCTTGGAGTCCA+TGG | + | Chr3:102145729-102145748 | None:intergenic | 50.0% | |
| CCGAATCCATGGACTCCAAG+AGG | - | Chr3:102145720-102145739 | MsG0380017914.01.T01:CDS | 55.0% | |
| CTTCTTCGAACCAACGGCAC+CGG | + | Chr3:102145593-102145612 | None:intergenic | 55.0% | |
| ATTCGGTCGCACTGAGGAGG+AGG | + | Chr3:102145566-102145585 | None:intergenic | 60.0% | |
| GGCATTCGGTCGCACTGAGG+AGG | + | Chr3:102145569-102145588 | None:intergenic | 65.0% | |
| TCAGTGCGACCGAATGCCGC+CGG | - | Chr3:102145571-102145590 | MsG0380017914.01.T01:CDS | 65.0% | |
| ! | CTTCGAACCAACGGCACCGG+CGG | + | Chr3:102145590-102145609 | None:intergenic | 65.0% |
| GGCGGCATTCGGTCGCACTG+AGG | + | Chr3:102145572-102145591 | None:intergenic | 70.0% | |
| !! | CCAACGGCACCGGCGGCATT+CGG | + | Chr3:102145583-102145602 | None:intergenic | 70.0% |
| !! | CCGAATGCCGCCGGTGCCGT+TGG | - | Chr3:102145580-102145599 | MsG0380017914.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 102145556 | 102145879 | 102145556 | ID=MsG0380017914.01;Name=MsG0380017914.01 |
| Chr3 | mRNA | 102145556 | 102145879 | 102145556 | ID=MsG0380017914.01.T01;Parent=MsG0380017914.01;Name=MsG0380017914.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|107 |
| Chr3 | exon | 102145556 | 102145879 | 102145556 | ID=MsG0380017914.01.T01:exon:35939;Parent=MsG0380017914.01.T01 |
| Chr3 | CDS | 102145556 | 102145879 | 102145556 | ID=MsG0380017914.01.T01:cds;Parent=MsG0380017914.01.T01 |
| Gene Sequence |
| Protein sequence |
