Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006682.01.T01 | AFK40878.1 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 5.04E-69 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006682.01.T01 | Q84WK5 | 80.556 | 108 | 20 | 1 | 1 | 107 | 1 | 108 | 1.87E-54 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006682.01.T01 | I3SKT6 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 2.41e-69 | 216 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0280006682.01 | 0.824330 | 8.559146e-54 | 7.557437e-51 |
| MsG0080048197.01 | MsG0280006682.01 | 0.813701 | 2.240728e-51 | 1.473769e-48 |
| MsG0080048843.01 | MsG0280006682.01 | 0.810387 | 1.181953e-50 | 7.114755e-48 |
| MsG0080048968.01 | MsG0280006682.01 | 0.820976 | 5.160360e-53 | 4.145026e-50 |
| MsG0180000023.01 | MsG0280006682.01 | 0.810560 | 1.084974e-50 | 6.561240e-48 |
| MsG0180000157.01 | MsG0280006682.01 | 0.823032 | 1.722972e-53 | 1.466712e-50 |
| MsG0180000611.01 | MsG0280006682.01 | 0.838662 | 2.516735e-57 | 3.394127e-54 |
| MsG0180000680.01 | MsG0280006682.01 | 0.802389 | 5.742879e-49 | 2.809762e-46 |
| MsG0180001043.01 | MsG0280006682.01 | 0.847750 | 9.493445e-60 | 1.704235e-56 |
| MsG0180001599.01 | MsG0280006682.01 | 0.814085 | 1.843384e-51 | 1.225099e-48 |
| MsG0180001712.01 | MsG0280006682.01 | 0.804065 | 2.583729e-49 | 1.319523e-46 |
| MsG0180001922.01 | MsG0280006682.01 | 0.833088 | 6.518964e-56 | 7.429774e-53 |
| MsG0180002140.01 | MsG0280006682.01 | 0.839452 | 1.571339e-57 | 2.170975e-54 |
| MsG0180002283.01 | MsG0280006682.01 | 0.810740 | 9.917599e-51 | 6.026749e-48 |
| MsG0180002491.01 | MsG0280006682.01 | 0.806908 | 6.546558e-50 | 3.596379e-47 |
| MsG0180003181.01 | MsG0280006682.01 | 0.818225 | 2.190280e-52 | 1.629841e-49 |
| MsG0180003252.01 | MsG0280006682.01 | 0.806366 | 8.516785e-50 | 4.614160e-47 |
| MsG0180003303.01 | MsG0280006682.01 | 0.817612 | 3.013323e-52 | 2.204895e-49 |
| MsG0180003379.01 | MsG0280006682.01 | 0.823374 | 1.433825e-53 | 1.232212e-50 |
| MsG0180003744.01 | MsG0280006682.01 | 0.821769 | 3.385116e-53 | 2.779852e-50 |
| MsG0180004016.01 | MsG0280006682.01 | 0.854023 | 1.622344e-61 | 3.574506e-58 |
| MsG0180004128.01 | MsG0280006682.01 | 0.803783 | 2.956486e-49 | 1.498941e-46 |
| MsG0180004437.01 | MsG0280006682.01 | 0.802158 | 6.405837e-49 | 3.115618e-46 |
| MsG0180004442.01 | MsG0280006682.01 | 0.807944 | 3.946088e-50 | 2.227071e-47 |
| MsG0180004444.01 | MsG0280006682.01 | 0.825684 | 4.099617e-54 | 3.763076e-51 |
| MsG0180004598.01 | MsG0280006682.01 | 0.804193 | 2.428937e-49 | 1.244483e-46 |
| MsG0180004767.01 | MsG0280006682.01 | 0.822738 | 2.017579e-53 | 1.703283e-50 |
| MsG0180005106.01 | MsG0280006682.01 | 0.849079 | 4.069485e-60 | 7.626071e-57 |
| MsG0180005125.01 | MsG0280006682.01 | 0.806720 | 7.172535e-50 | 3.921266e-47 |
| MsG0180005268.01 | MsG0280006682.01 | 0.833784 | 4.369831e-56 | 5.085083e-53 |
| MsG0180005787.01 | MsG0280006682.01 | 0.821804 | 3.322113e-53 | 2.730855e-50 |
| MsG0180006016.01 | MsG0280006682.01 | 0.804083 | 2.561404e-49 | 1.308687e-46 |
| MsG0180006046.01 | MsG0280006682.01 | 0.810097 | 1.365651e-50 | 8.158581e-48 |
| MsG0180006050.01 | MsG0280006682.01 | 0.817762 | 2.787659e-52 | 2.048264e-49 |
| MsG0280006302.01 | MsG0280006682.01 | -0.804942 | 1.695751e-49 | 8.858257e-47 |
| MsG0280006367.01 | MsG0280006682.01 | 0.804739 | 1.869703e-49 | 9.716120e-47 |
| MsG0280006368.01 | MsG0280006682.01 | 0.804711 | 1.894495e-49 | 9.837450e-47 |
| MsG0280006519.01 | MsG0280006682.01 | 0.831633 | 1.494253e-55 | 1.630955e-52 |
| MsG0280006679.01 | MsG0280006682.01 | 0.826668 | 2.391363e-54 | 2.257419e-51 |
| MsG0280006682.01 | MsG0280006892.01 | 0.832664 | 8.310891e-56 | 9.352292e-53 |
| MsG0280006682.01 | MsG0280006935.01 | 0.807735 | 4.371703e-50 | 2.453882e-47 |
| MsG0280006682.01 | MsG0280007441.01 | 0.806798 | 6.905185e-50 | 3.782642e-47 |
| MsG0280006682.01 | MsG0280007549.01 | 0.802729 | 4.886663e-49 | 2.411635e-46 |
| MsG0280006682.01 | MsG0280007714.01 | 0.808134 | 3.594783e-50 | 2.039086e-47 |
| MsG0280006682.01 | MsG0280007775.01 | 0.804165 | 2.461850e-49 | 1.260465e-46 |
| MsG0280006682.01 | MsG0280009554.01 | 0.839617 | 1.423776e-57 | 1.976945e-54 |
| MsG0280006682.01 | MsG0280010416.01 | 0.816107 | 6.558586e-52 | 4.605469e-49 |
| MsG0280006682.01 | MsG0280010423.01 | -0.810175 | 1.313746e-50 | 7.864267e-48 |
| MsG0280006682.01 | MsG0280011235.01 | 0.807682 | 4.485962e-50 | 2.514460e-47 |
| MsG0280006682.01 | MsG0380011614.01 | 0.837739 | 4.351056e-57 | 5.705263e-54 |
| MsG0280006682.01 | MsG0380012187.01 | 0.803178 | 3.945225e-49 | 1.969419e-46 |
| MsG0280006682.01 | MsG0380012458.01 | 0.806649 | 7.422788e-50 | 4.050365e-47 |
| MsG0280006682.01 | MsG0380014330.01 | 0.805571 | 1.251635e-49 | 6.644405e-47 |
| MsG0280006682.01 | MsG0380014908.01 | 0.805018 | 1.634408e-49 | 8.554050e-47 |
| MsG0280006682.01 | MsG0380015560.01 | 0.814539 | 1.463616e-51 | 9.847569e-49 |
| MsG0280006682.01 | MsG0380015607.01 | 0.851140 | 1.077514e-60 | 2.159976e-57 |
| MsG0280006682.01 | MsG0380015818.01 | 0.809898 | 1.507268e-50 | 8.957732e-48 |
| MsG0280006682.01 | MsG0380016117.01 | 0.853742 | 1.954460e-61 | 4.265764e-58 |
| MsG0280006682.01 | MsG0380016223.01 | 0.803149 | 4.000992e-49 | 1.995721e-46 |
| MsG0280006682.01 | MsG0380017003.01 | 0.818051 | 2.397796e-52 | 1.775723e-49 |
| MsG0280006682.01 | MsG0380017210.01 | 0.811500 | 6.788505e-51 | 4.209134e-48 |
| MsG0280006682.01 | MsG0380017279.01 | 0.819437 | 1.162192e-52 | 8.942985e-50 |
| MsG0280006682.01 | MsG0380017476.01 | 0.837356 | 5.454606e-57 | 7.067112e-54 |
| MsG0280006682.01 | MsG0380017512.01 | 0.840274 | 9.594838e-58 | 1.359751e-54 |
| MsG0280006682.01 | MsG0380017604.01 | 0.809865 | 1.531923e-50 | 9.096609e-48 |
| MsG0280006682.01 | MsG0380017941.01 | 0.811605 | 6.438447e-51 | 4.002880e-48 |
| MsG0280006682.01 | MsG0480018321.01 | 0.808671 | 2.762067e-50 | 1.588857e-47 |
| MsG0280006682.01 | MsG0480018332.01 | 0.839290 | 1.731603e-57 | 2.380314e-54 |
| MsG0280006682.01 | MsG0480018906.01 | 0.803209 | 3.888757e-49 | 1.942695e-46 |
| MsG0280006682.01 | MsG0480019289.01 | 0.824198 | 9.193328e-54 | 8.087294e-51 |
| MsG0280006682.01 | MsG0480019642.01 | 0.800286 | 1.547921e-48 | 7.179880e-46 |
| MsG0280006682.01 | MsG0480020144.01 | 0.806832 | 6.791858e-50 | 3.723917e-47 |
| MsG0280006682.01 | MsG0480020318.01 | 0.807679 | 4.493187e-50 | 2.518326e-47 |
| MsG0280006682.01 | MsG0480020487.01 | 0.800367 | 1.489965e-48 | 6.925370e-46 |
| MsG0280006682.01 | MsG0480020719.01 | 0.834210 | 3.418729e-56 | 4.029191e-53 |
| MsG0280006682.01 | MsG0480020843.01 | 0.839769 | 1.299388e-57 | 1.812674e-54 |
| MsG0280006682.01 | MsG0480021041.01 | 0.814087 | 1.842205e-51 | 1.224357e-48 |
| MsG0280006682.01 | MsG0480021190.01 | 0.803322 | 3.684414e-49 | 1.845931e-46 |
| MsG0280006682.01 | MsG0480021240.01 | 0.812511 | 4.086724e-51 | 2.603477e-48 |
| MsG0280006682.01 | MsG0480022108.01 | 0.805229 | 1.476667e-49 | 7.770284e-47 |
| MsG0280006682.01 | MsG0480022493.01 | 0.819413 | 1.176884e-52 | 9.050024e-50 |
| MsG0280006682.01 | MsG0480022597.01 | 0.810013 | 1.423133e-50 | 8.484004e-48 |
| MsG0280006682.01 | MsG0480022678.01 | 0.817660 | 2.939367e-52 | 2.153641e-49 |
| MsG0280006682.01 | MsG0480023032.01 | 0.807194 | 5.695191e-50 | 3.152021e-47 |
| MsG0280006682.01 | MsG0480023136.01 | -0.805136 | 1.544518e-49 | 8.108178e-47 |
| MsG0280006682.01 | MsG0480023240.01 | 0.827641 | 1.398598e-54 | 1.358084e-51 |
| MsG0280006682.01 | MsG0480023377.01 | 0.806876 | 6.647684e-50 | 3.648994e-47 |
| MsG0280006682.01 | MsG0480023517.01 | 0.803256 | 3.801724e-49 | 1.901530e-46 |
| MsG0280006682.01 | MsG0480023554.01 | 0.819619 | 1.055882e-52 | 8.166123e-50 |
| MsG0280006682.01 | MsG0480023841.01 | -0.821903 | 3.152650e-53 | 2.598808e-50 |
| MsG0280006682.01 | MsG0580024191.01 | 0.800904 | 1.158015e-48 | 5.456410e-46 |
| MsG0280006682.01 | MsG0580024581.01 | 0.826020 | 3.411657e-54 | 3.161697e-51 |
| MsG0280006682.01 | MsG0580025134.01 | 0.803105 | 4.085113e-49 | 2.035285e-46 |
| MsG0280006682.01 | MsG0580025278.01 | 0.811133 | 8.151981e-51 | 5.005814e-48 |
| MsG0280006682.01 | MsG0580025400.01 | 0.812079 | 5.078472e-51 | 3.197784e-48 |
| MsG0280006682.01 | MsG0580025769.01 | 0.809977 | 1.449220e-50 | 8.630950e-48 |
| MsG0280006682.01 | MsG0580027034.01 | 0.825124 | 5.560521e-54 | 5.023341e-51 |
| MsG0280006682.01 | MsG0580028526.01 | 0.807852 | 4.127824e-50 | 2.323968e-47 |
| MsG0280006682.01 | MsG0580029283.01 | 0.812147 | 4.907390e-51 | 3.095845e-48 |
| MsG0280006682.01 | MsG0580029628.01 | 0.820763 | 5.775350e-53 | 4.611220e-50 |
| MsG0280006682.01 | MsG0580029783.01 | 0.810310 | 1.228556e-50 | 7.380331e-48 |
| MsG0280006682.01 | MsG0580029801.01 | 0.828429 | 9.036759e-55 | 8.980229e-52 |
| MsG0280006682.01 | MsG0580030204.01 | 0.822204 | 2.684819e-53 | 2.232175e-50 |
| MsG0280006682.01 | MsG0680030841.01 | 0.801154 | 1.029287e-48 | 4.880594e-46 |
| MsG0280006682.01 | MsG0680031051.01 | 0.830484 | 2.862177e-55 | 3.019368e-52 |
| MsG0280006682.01 | MsG0680031536.01 | 0.859543 | 3.846469e-63 | 1.019504e-59 |
| MsG0280006682.01 | MsG0680031538.01 | 0.824590 | 7.431660e-54 | 6.612317e-51 |
| MsG0280006682.01 | MsG0680033926.01 | 0.812900 | 3.358407e-51 | 2.161507e-48 |
| MsG0280006682.01 | MsG0680034476.01 | 0.828365 | 9.362295e-55 | 9.285852e-52 |
| MsG0280006682.01 | MsG0680035854.01 | 0.819230 | 1.295219e-52 | 9.909308e-50 |
| MsG0280006682.01 | MsG0780036247.01 | -0.818215 | 2.201810e-52 | 1.637981e-49 |
| MsG0280006682.01 | MsG0780036845.01 | 0.811219 | 7.811689e-51 | 4.807584e-48 |
| MsG0280006682.01 | MsG0780039340.01 | 0.817139 | 3.850635e-52 | 2.781388e-49 |
| MsG0280006682.01 | MsG0780039587.01 | 0.813370 | 2.649324e-51 | 1.726972e-48 |
| MsG0280006682.01 | MsG0780039943.01 | 0.856064 | 4.140455e-62 | 9.760275e-59 |
| MsG0280006682.01 | MsG0780040125.01 | 0.845443 | 4.047934e-59 | 6.746796e-56 |
| MsG0280006682.01 | MsG0780040190.01 | 0.818812 | 1.612268e-52 | 1.219325e-49 |
| MsG0280006682.01 | MsG0780040424.01 | 0.806492 | 8.012930e-50 | 4.355161e-47 |
| MsG0280006682.01 | MsG0780040742.01 | 0.822103 | 2.833222e-53 | 2.348722e-50 |
| MsG0280006682.01 | MsG0780041294.01 | 0.811926 | 5.482430e-51 | 3.437631e-48 |
| MsG0280006682.01 | MsG0780041423.01 | 0.801185 | 1.014616e-48 | 4.814567e-46 |
| MsG0280006682.01 | MsG0880042033.01 | 0.808154 | 3.559533e-50 | 2.020195e-47 |
| MsG0280006682.01 | MsG0880042399.01 | -0.855830 | 4.848036e-62 | 1.133955e-58 |
| MsG0280006682.01 | MsG0880043082.01 | 0.817401 | 3.360742e-52 | 2.445041e-49 |
| MsG0280006682.01 | MsG0880043162.01 | 0.834549 | 2.810984e-56 | 3.347114e-53 |
| MsG0280006682.01 | MsG0880043194.01 | 0.821197 | 4.587941e-53 | 3.707932e-50 |
| MsG0280006682.01 | MsG0880044409.01 | 0.808969 | 2.384537e-50 | 1.382574e-47 |
| MsG0280006682.01 | MsG0880045106.01 | 0.800761 | 1.238290e-48 | 5.813079e-46 |
| MsG0280006682.01 | MsG0880045165.01 | 0.806013 | 1.010662e-49 | 5.426661e-47 |
| MsG0280006682.01 | MsG0880045656.01 | 0.810607 | 1.059877e-50 | 6.417620e-48 |
| MsG0280006682.01 | MsG0880045756.01 | 0.807590 | 4.692804e-50 | 2.623988e-47 |
| MsG0280006682.01 | MsG0880045914.01 | 0.802425 | 5.644235e-49 | 2.764040e-46 |
| MsG0280006682.01 | MsG0880046058.01 | 0.821061 | 4.932253e-53 | 3.971179e-50 |
| MsG0280006682.01 | MsG0880046068.01 | 0.819158 | 1.344891e-52 | 1.026886e-49 |
| MsG0280006682.01 | MsG0880046091.01 | 0.812889 | 3.377104e-51 | 2.172911e-48 |
| MsG0280006682.01 | MsG0880046209.01 | 0.803488 | 3.403468e-49 | 1.712546e-46 |
| MsG0280006682.01 | MsG0880046244.01 | 0.803795 | 2.938735e-49 | 1.490416e-46 |
| MsG0280006682.01 | MsG0880046342.01 | 0.803177 | 3.947771e-49 | 1.970608e-46 |
| MsG0280006682.01 | MsG0880046377.01 | 0.803661 | 3.133883e-49 | 1.583851e-46 |
| MsG0280006682.01 | MsG0880046921.01 | 0.801936 | 7.117346e-49 | 3.442032e-46 |
| MsG0280006682.01 | MsG0880047019.01 | 0.822248 | 2.622334e-53 | 2.183003e-50 |
| MsG0280006682.01 | MsG0880047116.01 | 0.801532 | 8.612261e-49 | 4.122698e-46 |
| MsG0280006682.01 | MsG0880047328.01 | -0.816657 | 4.939163e-52 | 3.520961e-49 |
| MsG0280006682.01 | MsG0880047428.01 | 0.809901 | 1.504591e-50 | 8.942869e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006682.01.T01 | MTR_2g012810 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 4.64e-72 | 215 |
| MsG0280006682.01.T01 | MTR_2g012810 | 98.462 | 65 | 1 | 0 | 43 | 107 | 1 | 65 | 1.68e-40 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006682.01.T01 | AT1G61150 | 80.556 | 108 | 20 | 1 | 1 | 107 | 1 | 108 | 1.90e-55 | 172 |
| MsG0280006682.01.T01 | AT1G61150 | 80.556 | 108 | 20 | 1 | 1 | 107 | 1 | 108 | 1.90e-55 | 172 |
| MsG0280006682.01.T01 | AT1G61150 | 80.556 | 108 | 20 | 1 | 1 | 107 | 1 | 108 | 1.90e-55 | 172 |
| MsG0280006682.01.T01 | AT1G61150 | 85.714 | 91 | 13 | 0 | 17 | 107 | 1 | 91 | 3.52e-52 | 164 |
| MsG0280006682.01.T01 | AT1G61150 | 85.714 | 91 | 13 | 0 | 17 | 107 | 1 | 91 | 3.52e-52 | 164 |
| MsG0280006682.01.T01 | AT1G61150 | 85.714 | 91 | 13 | 0 | 17 | 107 | 1 | 91 | 3.52e-52 | 164 |
| MsG0280006682.01.T01 | AT1G61150 | 85.714 | 91 | 13 | 0 | 17 | 107 | 1 | 91 | 3.52e-52 | 164 |
| MsG0280006682.01.T01 | AT1G61150 | 85.714 | 91 | 13 | 0 | 17 | 107 | 1 | 91 | 3.52e-52 | 164 |
| MsG0280006682.01.T01 | AT1G61150 | 85.882 | 85 | 12 | 0 | 23 | 107 | 1 | 85 | 1.18e-48 | 155 |
| MsG0280006682.01.T01 | AT1G11110 | 56.818 | 88 | 38 | 0 | 20 | 107 | 45 | 132 | 1.74e-32 | 115 |
| MsG0280006682.01.T01 | AT1G11110 | 56.818 | 88 | 38 | 0 | 20 | 107 | 52 | 139 | 2.76e-32 | 114 |
| MsG0280006682.01.T01 | AT1G11110 | 59.494 | 79 | 32 | 0 | 29 | 107 | 5 | 83 | 9.28e-32 | 112 |
| MsG0280006682.01.T01 | AT4G09300 | 57.143 | 91 | 39 | 0 | 17 | 107 | 1 | 91 | 1.09e-29 | 106 |
| MsG0280006682.01.T01 | AT4G09300 | 57.143 | 91 | 39 | 0 | 17 | 107 | 1 | 91 | 1.09e-29 | 106 |
| MsG0280006682.01.T01 | AT4G09300 | 57.303 | 89 | 38 | 0 | 19 | 107 | 8 | 96 | 4.86e-29 | 105 |
| MsG0280006682.01.T01 | AT4G09300 | 56.044 | 91 | 39 | 1 | 17 | 107 | 1 | 90 | 6.01e-27 | 99.8 |
| MsG0280006682.01.T01 | AT1G61150 | 91.667 | 36 | 3 | 0 | 72 | 107 | 23 | 58 | 2.71e-16 | 71.2 |
| MsG0280006682.01.T01 | AT1G61150 | 91.667 | 36 | 3 | 0 | 72 | 107 | 23 | 58 | 2.71e-16 | 71.2 |
Find 29 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGGATTGGTGCGTCATTT+TGG | 0.210516 | 2:+4914038 | MsG0280006682.01.T01:five_prime_UTR |
| GAAAGAAGACATGAATAAAT+TGG | 0.301615 | 2:+4915062 | MsG0280006682.01.T01:CDS |
| AAATTCATTCTCGTATAAAA+AGG | 0.308936 | 2:-4915384 | None:intergenic |
| GTTCACTGATGTCATTGTTT+TGG | 0.318337 | 2:+4914082 | MsG0280006682.01.T01:exon |
| GAGAAATTTCGCAAGGAGTC+TGG | 0.335188 | 2:+4915126 | MsG0280006682.01.T01:CDS |
| TCTGAATTTCGATAACTTGC+TGG | 0.336256 | 2:-4913833 | None:intergenic |
| ACTTTGTACTGCCTTCTTAA+CGG | 0.366231 | 2:-4915299 | None:intergenic |
| TTCTAGAATTCTCCGATTTG+AGG | 0.395401 | 2:+4913888 | MsG0280006682.01.T01:five_prime_UTR |
| AGGTGATAACAAGGGAAGAG+TGG | 0.458996 | 2:+4915010 | MsG0280006682.01.T01:CDS |
| GGTGATAACAAGGGAAGAGT+GGG | 0.465327 | 2:+4915011 | MsG0280006682.01.T01:CDS |
| AGCTTAACAATGTGAAGATT+AGG | 0.468637 | 2:+4915040 | MsG0280006682.01.T01:CDS |
| TGATCGGATGGCCGTTAAGA+AGG | 0.485549 | 2:+4915288 | MsG0280006682.01.T01:CDS |
| AGAGATAATTCACCTCAAAT+CGG | 0.504118 | 2:-4913900 | None:intergenic |
| TTTCAACTTCTTAAATCGTA+CGG | 0.513264 | 2:-4913859 | None:intergenic |
| TGTAAAGGCGGCATCAAAGA+AGG | 0.522077 | 2:+4914990 | MsG0280006682.01.T01:intron |
| AGGAATAGCAAGCTCACCTC+AGG | 0.523136 | 2:-4915364 | None:intergenic |
| ATGAATTTCCTTGTCACCGA+GGG | 0.535234 | 2:+4915087 | MsG0280006682.01.T01:CDS |
| TCGTCAGCTAGCACAAATTG+AGG | 0.540623 | 2:+4914113 | MsG0280006682.01.T01:CDS |
| CAACGAAGCCCTCGGTGACA+AGG | 0.542326 | 2:-4915095 | None:intergenic |
| TGCTACAATTACTGATCGGA+TGG | 0.544023 | 2:+4915276 | MsG0280006682.01.T01:CDS |
| GCTAGCACAAATTGAGGCAA+TGG | 0.559479 | 2:+4914119 | MsG0280006682.01.T01:CDS |
| CGCTGCAGAGAAATTTCGCA+AGG | 0.565277 | 2:+4915119 | MsG0280006682.01.T01:CDS |
| TGCAGCGTCAACGAAGCCCT+CGG | 0.574011 | 2:-4915103 | None:intergenic |
| CATCAAAGAAGGTGATAACA+AGG | 0.577900 | 2:+4915001 | MsG0280006682.01.T01:CDS |
| ATCTTGCTACAATTACTGAT+CGG | 0.583783 | 2:+4915272 | MsG0280006682.01.T01:CDS |
| GTTAAGAAGGCAGTACAAAG+TGG | 0.657413 | 2:+4915301 | MsG0280006682.01.T01:CDS |
| AGTCAATGACTTAAATCCTG+AGG | 0.660738 | 2:+4915348 | MsG0280006682.01.T01:CDS |
| ATCAAAGAAGGTGATAACAA+GGG | 0.690079 | 2:+4915002 | MsG0280006682.01.T01:CDS |
| AATGAATTTCCTTGTCACCG+AGG | 0.703485 | 2:+4915086 | MsG0280006682.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTATTATTATTCTTTG+GGG | + | Chr2:4915187-4915206 | MsG0280006682.01.T01:intron | 10.0% |
| !!! | AAAAGTATATATTTTTAATG+TGG | + | Chr2:4914237-4914256 | MsG0280006682.01.T01:intron | 10.0% |
| !!! | AAAGTATATATTTTTAATGT+GGG | + | Chr2:4914238-4914257 | MsG0280006682.01.T01:intron | 10.0% |
| !! | TTATTATTATTATTCTTTGG+GGG | + | Chr2:4915188-4915207 | MsG0280006682.01.T01:intron | 15.0% |
| !!! | ATTGAATTGTTCAAATTTTG+AGG | + | Chr2:4914020-4914039 | MsG0280006682.01.T01:five_prime_UTR | 20.0% |
| ! | ACAAATCCGCATATAAAATT+CGG | - | Chr2:4914197-4914216 | None:intergenic | 25.0% |
| ! | ACATTAAAATCACAACGAAA+TGG | - | Chr2:4914638-4914657 | None:intergenic | 25.0% |
| ! | ATATGAGTTTGTAGTTTACA+TGG | + | Chr2:4914729-4914748 | MsG0280006682.01.T01:intron | 25.0% |
| ! | GAAAGAAGACATGAATAAAT+TGG | + | Chr2:4915062-4915081 | MsG0280006682.01.T01:CDS | 25.0% |
| ! | GGAATATAAAAGAAAACATC+AGG | - | Chr2:4914617-4914636 | None:intergenic | 25.0% |
| ! | TTTCAACTTCTTAAATCGTA+CGG | - | Chr2:4913862-4913881 | None:intergenic | 25.0% |
| !! | TTTTTCCCTTTCGTTAATTA+GGG | + | Chr2:4913940-4913959 | MsG0280006682.01.T01:intron | 25.0% |
| !!! | ATTGTTCAAATTTTGAGGAT+TGG | + | Chr2:4914025-4914044 | MsG0280006682.01.T01:five_prime_UTR | 25.0% |
| !!! | ATTTCGTTGTGATTTTAATG+TGG | + | Chr2:4914637-4914656 | MsG0280006682.01.T01:intron | 25.0% |
| !!! | TCTTTGAAATACATGTGTAA+AGG | + | Chr2:4914975-4914994 | MsG0280006682.01.T01:intron | 25.0% |
| !!! | TGACAATATTTTAGAGAGTT+GGG | + | Chr2:4914325-4914344 | MsG0280006682.01.T01:intron | 25.0% |
| !!! | TTTTTTCCCTTTCGTTAATT+AGG | + | Chr2:4913939-4913958 | MsG0280006682.01.T01:intron | 25.0% |
| AAATCCGCATATAAAATTCG+GGG | - | Chr2:4914195-4914214 | None:intergenic | 30.0% | |
| AGAGATAATTCACCTCAAAT+CGG | - | Chr2:4913903-4913922 | None:intergenic | 30.0% | |
| AGCTTAACAATGTGAAGATT+AGG | + | Chr2:4915040-4915059 | MsG0280006682.01.T01:CDS | 30.0% | |
| ATCAAAATCGTCACATTCTT+CGG | - | Chr2:4913988-4914007 | None:intergenic | 30.0% | |
| ATCAAAGAAGGTGATAACAA+GGG | + | Chr2:4915002-4915021 | MsG0280006682.01.T01:CDS | 30.0% | |
| ATCTTGCTACAATTACTGAT+CGG | + | Chr2:4915272-4915291 | MsG0280006682.01.T01:CDS | 30.0% | |
| CAAATCCGCATATAAAATTC+GGG | - | Chr2:4914196-4914215 | None:intergenic | 30.0% | |
| GTAAAGTCGAGTTTCTAAAT+AGG | - | Chr2:4914357-4914376 | None:intergenic | 30.0% | |
| TAGTCAATATTGTGCATCAT+GGG | + | Chr2:4914894-4914913 | MsG0280006682.01.T01:intron | 30.0% | |
| TGAAACCCTAATTAACGAAA+GGG | - | Chr2:4913948-4913967 | None:intergenic | 30.0% | |
| TTGAAACCCTAATTAACGAA+AGG | - | Chr2:4913949-4913968 | None:intergenic | 30.0% | |
| TTGAAATACATGTGTAAAGG+CGG | + | Chr2:4914978-4914997 | MsG0280006682.01.T01:intron | 30.0% | |
| TTTGTGCTTGTTTAAGAGTT+CGG | + | Chr2:4914280-4914299 | MsG0280006682.01.T01:intron | 30.0% | |
| ! | AAGTGTTTCTATATACGAAC+TGG | + | Chr2:4914868-4914887 | MsG0280006682.01.T01:intron | 30.0% |
| ! | TATTATTATTCTTTGGGGGA+GGG | + | Chr2:4915192-4915211 | MsG0280006682.01.T01:intron | 30.0% |
| ! | TTATTATTATTCTTTGGGGG+AGG | + | Chr2:4915191-4915210 | MsG0280006682.01.T01:intron | 30.0% |
| ! | TTCCTTTTTATACGAGAATG+AGG | + | Chr2:4915382-4915401 | MsG0280006682.01.T01:CDS | 30.0% |
| !! | GACAATATTTTAGAGAGTTG+GGG | + | Chr2:4914326-4914345 | MsG0280006682.01.T01:intron | 30.0% |
| !! | GTGACAATATTTTAGAGAGT+TGG | + | Chr2:4914324-4914343 | MsG0280006682.01.T01:intron | 30.0% |
| !! | TTAATTTTCTAGAAATGCCC+TGG | + | Chr2:4914697-4914716 | MsG0280006682.01.T01:intron | 30.0% |
| !!! | TTCTGTCATTTTGCATAAGA+CGG | + | Chr2:4914814-4914833 | MsG0280006682.01.T01:intron | 30.0% |
| AACTCATATCTTCACTTTCC+AGG | - | Chr2:4914718-4914737 | None:intergenic | 35.0% | |
| AAGGGTAGTAAAATCACTAG+AGG | + | Chr2:4914438-4914457 | MsG0280006682.01.T01:intron | 35.0% | |
| AATCCGCATATAAAATTCGG+GGG | - | Chr2:4914194-4914213 | None:intergenic | 35.0% | |
| ACCATTACCATGAAACACAT+GGG | - | Chr2:4914939-4914958 | None:intergenic | 35.0% | |
| ACTCATATCTTCACTTTCCA+GGG | - | Chr2:4914717-4914736 | None:intergenic | 35.0% | |
| ACTTTGTACTGCCTTCTTAA+CGG | - | Chr2:4915302-4915321 | None:intergenic | 35.0% | |
| AGTAAAATCACTAGAGGTAG+AGG | + | Chr2:4914444-4914463 | MsG0280006682.01.T01:intron | 35.0% | |
| AGTCAATGACTTAAATCCTG+AGG | + | Chr2:4915348-4915367 | MsG0280006682.01.T01:CDS | 35.0% | |
| ATGTCAGCTGAAGATCATTA+AGG | - | Chr2:4915250-4915269 | None:intergenic | 35.0% | |
| ATTATGACGTTGTTTGATCC+AGG | + | Chr2:4914539-4914558 | MsG0280006682.01.T01:intron | 35.0% | |
| CATCAAAGAAGGTGATAACA+AGG | + | Chr2:4915001-4915020 | MsG0280006682.01.T01:CDS | 35.0% | |
| GAGTTTGTAGTTTACATGGA+AGG | + | Chr2:4914733-4914752 | MsG0280006682.01.T01:intron | 35.0% | |
| GTAGTCAATATTGTGCATCA+TGG | + | Chr2:4914893-4914912 | MsG0280006682.01.T01:intron | 35.0% | |
| TACCATTACCATGAAACACA+TGG | - | Chr2:4914940-4914959 | None:intergenic | 35.0% | |
| TGTCAGCTGAAGATCATTAA+GGG | - | Chr2:4915249-4915268 | None:intergenic | 35.0% | |
| ! | ATTATTATTCTTTGGGGGAG+GGG | + | Chr2:4915193-4915212 | MsG0280006682.01.T01:intron | 35.0% |
| ! | CTTTACATGTTTTGCGCATT+TGG | + | Chr2:4914371-4914390 | MsG0280006682.01.T01:intron | 35.0% |
| ! | GACTTGTAGATTTTGTAGCA+CGG | + | Chr2:4914400-4914419 | MsG0280006682.01.T01:intron | 35.0% |
| ! | GTTCACTGATGTCATTGTTT+TGG | + | Chr2:4914082-4914101 | MsG0280006682.01.T01:exon | 35.0% |
| ! | GTTTTTGCGATGATTGAGTT+CGG | - | Chr2:4913808-4913827 | None:intergenic | 35.0% |
| ! | TCTGAATTTCGATAACTTGC+TGG | - | Chr2:4913836-4913855 | None:intergenic | 35.0% |
| ! | TTTCCCCCGAATTTTATATG+CGG | + | Chr2:4914188-4914207 | MsG0280006682.01.T01:intron | 35.0% |
| !! | TTCTAGAATTCTCCGATTTG+AGG | + | Chr2:4913888-4913907 | MsG0280006682.01.T01:five_prime_UTR | 35.0% |
| AATGAATTTCCTTGTCACCG+AGG | + | Chr2:4915086-4915105 | MsG0280006682.01.T01:CDS | 40.0% | |
| AATTGAGGCAATGGTATGTG+TGG | + | Chr2:4914128-4914147 | MsG0280006682.01.T01:intron | 40.0% | |
| ACGGAGAGTAGTTCAAACAA+AGG | + | Chr2:4914419-4914438 | MsG0280006682.01.T01:intron | 40.0% | |
| AGGTAGTGTATCCCACTTAA+TGG | + | Chr2:4914559-4914578 | MsG0280006682.01.T01:intron | 40.0% | |
| AGTGATTCCCATGTGTTTCA+TGG | + | Chr2:4914929-4914948 | MsG0280006682.01.T01:intron | 40.0% | |
| ATATTGTGCATCATGGGTGA+TGG | + | Chr2:4914900-4914919 | MsG0280006682.01.T01:intron | 40.0% | |
| ATGAATTTCCTTGTCACCGA+GGG | + | Chr2:4915087-4915106 | MsG0280006682.01.T01:CDS | 40.0% | |
| ATTAAGTGGGATACACTACC+TGG | - | Chr2:4914560-4914579 | None:intergenic | 40.0% | |
| CGGAGAGTAGTTCAAACAAA+GGG | + | Chr2:4914420-4914439 | MsG0280006682.01.T01:intron | 40.0% | |
| GGTAGTGTATCCCACTTAAT+GGG | + | Chr2:4914560-4914579 | MsG0280006682.01.T01:intron | 40.0% | |
| GTTAAGAAGGCAGTACAAAG+TGG | + | Chr2:4915301-4915320 | MsG0280006682.01.T01:CDS | 40.0% | |
| TCCCATGTGTTTCATGGTAA+TGG | + | Chr2:4914935-4914954 | MsG0280006682.01.T01:intron | 40.0% | |
| TGCTACAATTACTGATCGGA+TGG | + | Chr2:4915276-4915295 | MsG0280006682.01.T01:CDS | 40.0% | |
| TGCTTGTTTAAGAGTTCGGA+TGG | + | Chr2:4914284-4914303 | MsG0280006682.01.T01:intron | 40.0% | |
| TTGTTTAAGAGTTCGGATGG+TGG | + | Chr2:4914287-4914306 | MsG0280006682.01.T01:intron | 40.0% | |
| ! | TTATTATTCTTTGGGGGAGG+GGG | + | Chr2:4915194-4915213 | MsG0280006682.01.T01:intron | 40.0% |
| !!! | CGTCATTTTGGGTTACAATG+AGG | + | Chr2:4914050-4914069 | MsG0280006682.01.T01:five_prime_UTR | 40.0% |
| ACCAGCTGTATCCCATTAAG+TGG | - | Chr2:4914574-4914593 | None:intergenic | 45.0% | |
| AGGCAATGGTATGTGTGGAT+TGG | + | Chr2:4914133-4914152 | MsG0280006682.01.T01:intron | 45.0% | |
| AGGTGATAACAAGGGAAGAG+TGG | + | Chr2:4915010-4915029 | MsG0280006682.01.T01:CDS | 45.0% | |
| CCAGCTGTATCCCATTAAGT+GGG | - | Chr2:4914573-4914592 | None:intergenic | 45.0% | |
| GAGAAATTTCGCAAGGAGTC+TGG | + | Chr2:4915126-4915145 | MsG0280006682.01.T01:CDS | 45.0% | |
| GCTAGCACAAATTGAGGCAA+TGG | + | Chr2:4914119-4914138 | MsG0280006682.01.T01:CDS | 45.0% | |
| GGTGATAACAAGGGAAGAGT+GGG | + | Chr2:4915011-4915030 | MsG0280006682.01.T01:CDS | 45.0% | |
| TCGTCAGCTAGCACAAATTG+AGG | + | Chr2:4914113-4914132 | MsG0280006682.01.T01:CDS | 45.0% | |
| TGTAAAGGCGGCATCAAAGA+AGG | + | Chr2:4914990-4915009 | MsG0280006682.01.T01:intron | 45.0% | |
| ! | TATTATTCTTTGGGGGAGGG+GGG | + | Chr2:4915195-4915214 | MsG0280006682.01.T01:intron | 45.0% |
| !! | GAGGATTGGTGCGTCATTTT+GGG | + | Chr2:4914039-4914058 | MsG0280006682.01.T01:five_prime_UTR | 45.0% |
| !! | TGAGGATTGGTGCGTCATTT+TGG | + | Chr2:4914038-4914057 | MsG0280006682.01.T01:five_prime_UTR | 45.0% |
| !! | AATAATAATAATAAGAAAAA+AGG | - | Chr2:4915164-4915183 | None:intergenic | 5.0% |
| !! | TATTATTATTATTATTCTTT+GGG | + | Chr2:4915186-4915205 | MsG0280006682.01.T01:intron | 5.0% |
| !! | TTATTATTATTATTATTCTT+TGG | + | Chr2:4915185-4915204 | MsG0280006682.01.T01:intron | 5.0% |
| AGGAATAGCAAGCTCACCTC+AGG | - | Chr2:4915367-4915386 | None:intergenic | 50.0% | |
| CCCACTTAATGGGATACAGC+TGG | + | Chr2:4914570-4914589 | MsG0280006682.01.T01:intron | 50.0% | |
| CGCTGCAGAGAAATTTCGCA+AGG | + | Chr2:4915119-4915138 | MsG0280006682.01.T01:CDS | 50.0% | |
| TGATCGGATGGCCGTTAAGA+AGG | + | Chr2:4915288-4915307 | MsG0280006682.01.T01:CDS | 50.0% | |
| !! | TCGGATGGTGGAAGTTGCGA+TGG | + | Chr2:4914299-4914318 | MsG0280006682.01.T01:intron | 55.0% |
| CAACGAAGCCCTCGGTGACA+AGG | - | Chr2:4915098-4915117 | None:intergenic | 60.0% | |
| TGCAGCGTCAACGAAGCCCT+CGG | - | Chr2:4915106-4915125 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 4913800 | 4915402 | 4913800 | ID=MsG0280006682.01;Name=MsG0280006682.01 |
| Chr2 | mRNA | 4913800 | 4915402 | 4913800 | ID=MsG0280006682.01.T01;Parent=MsG0280006682.01;Name=MsG0280006682.01.T01;_AED=0.45;_eAED=0.48;_QI=185|1|0.75|1|0.66|0.5|4|0|116 |
| Chr2 | exon | 4913800 | 4913909 | 4913800 | ID=MsG0280006682.01.T01:exon:1827;Parent=MsG0280006682.01.T01 |
| Chr2 | exon | 4914015 | 4914140 | 4914015 | ID=MsG0280006682.01.T01:exon:1828;Parent=MsG0280006682.01.T01 |
| Chr2 | exon | 4914997 | 4915156 | 4914997 | ID=MsG0280006682.01.T01:exon:1829;Parent=MsG0280006682.01.T01 |
| Chr2 | exon | 4915263 | 4915402 | 4915263 | ID=MsG0280006682.01.T01:exon:1830;Parent=MsG0280006682.01.T01 |
| Chr2 | five_prime_UTR | 4913800 | 4913909 | 4913800 | ID=MsG0280006682.01.T01:five_prime_utr;Parent=MsG0280006682.01.T01 |
| Chr2 | five_prime_UTR | 4914015 | 4914089 | 4914015 | ID=MsG0280006682.01.T01:five_prime_utr;Parent=MsG0280006682.01.T01 |
| Chr2 | CDS | 4914090 | 4914140 | 4914090 | ID=MsG0280006682.01.T01:cds;Parent=MsG0280006682.01.T01 |
| Chr2 | CDS | 4914997 | 4915156 | 4914997 | ID=MsG0280006682.01.T01:cds;Parent=MsG0280006682.01.T01 |
| Chr2 | CDS | 4915263 | 4915402 | 4915263 | ID=MsG0280006682.01.T01:cds;Parent=MsG0280006682.01.T01 |
| Gene Sequence |
| Protein sequence |
