Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015350.01.T01 | XP_013460702.1 | 78.182 | 275 | 11 | 2 | 1 | 226 | 1 | 275 | 4.18E-140 | 403 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015350.01.T01 | Q9LHT0 | 55.598 | 259 | 68 | 1 | 10 | 221 | 4 | 262 | 9.33E-95 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015350.01.T01 | A0A072UZ65 | 78.182 | 275 | 11 | 2 | 1 | 226 | 1 | 275 | 2.00e-140 | 403 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047974.01 | MsG0380015350.01 | 0.810261 | 1.258435e-50 | 7.550381e-48 |
| MsG0080048996.01 | MsG0380015350.01 | 0.845862 | 3.115551e-59 | 5.264258e-56 |
| MsG0080049140.01 | MsG0380015350.01 | 0.823783 | 1.150248e-53 | 1.000218e-50 |
| MsG0180000111.01 | MsG0380015350.01 | 0.801449 | 8.958807e-49 | 4.279557e-46 |
| MsG0180000275.01 | MsG0380015350.01 | 0.825565 | 4.373176e-54 | 4.000460e-51 |
| MsG0180002421.01 | MsG0380015350.01 | 0.809738 | 1.631244e-50 | 9.654615e-48 |
| MsG0180003427.01 | MsG0380015350.01 | 0.820389 | 7.040277e-53 | 5.563371e-50 |
| MsG0180003485.01 | MsG0380015350.01 | 0.804738 | 1.869835e-49 | 9.716771e-47 |
| MsG0180004099.01 | MsG0380015350.01 | 0.801145 | 1.033516e-48 | 4.899535e-46 |
| MsG0180004375.01 | MsG0380015350.01 | 0.805122 | 1.554827e-49 | 8.159475e-47 |
| MsG0180004661.01 | MsG0380015350.01 | 0.848908 | 4.541157e-60 | 8.462682e-57 |
| MsG0180004806.01 | MsG0380015350.01 | 0.802887 | 4.532508e-49 | 2.245814e-46 |
| MsG0180005002.01 | MsG0380015350.01 | 0.853801 | 1.879578e-61 | 4.110404e-58 |
| MsG0180006133.01 | MsG0380015350.01 | 0.800032 | 1.743432e-48 | 8.034523e-46 |
| MsG0280006311.01 | MsG0380015350.01 | 0.810873 | 9.281010e-51 | 5.660082e-48 |
| MsG0280010977.01 | MsG0380015350.01 | 0.819747 | 9.874276e-53 | 7.664497e-50 |
| MsG0380014808.01 | MsG0380015350.01 | 0.802539 | 5.346783e-49 | 2.625899e-46 |
| MsG0380015189.01 | MsG0380015350.01 | 0.811823 | 5.772600e-51 | 3.609820e-48 |
| MsG0380015350.01 | MsG0380015548.01 | 0.805230 | 1.476133e-49 | 7.767673e-47 |
| MsG0380015350.01 | MsG0380015816.01 | 0.803222 | 3.864192e-49 | 1.931085e-46 |
| MsG0380015350.01 | MsG0380015825.01 | 0.801318 | 9.527886e-49 | 4.536436e-46 |
| MsG0380015350.01 | MsG0380016225.01 | 0.806950 | 6.412126e-50 | 3.526424e-47 |
| MsG0380015350.01 | MsG0380016703.01 | 0.830911 | 2.249357e-55 | 2.403237e-52 |
| MsG0380015350.01 | MsG0380017159.01 | 0.829099 | 6.222224e-55 | 6.304984e-52 |
| MsG0380015350.01 | MsG0380017164.01 | 0.819587 | 1.074170e-52 | 8.300750e-50 |
| MsG0380015350.01 | MsG0380017240.01 | 0.813016 | 3.167608e-51 | 2.045022e-48 |
| MsG0380015350.01 | MsG0380017282.01 | 0.804876 | 1.750402e-49 | 9.128592e-47 |
| MsG0380015350.01 | MsG0480018397.01 | 0.834569 | 2.778257e-56 | 3.310313e-53 |
| MsG0380015350.01 | MsG0480018785.01 | 0.805425 | 1.343517e-49 | 7.105368e-47 |
| MsG0380015350.01 | MsG0480018918.01 | 0.819053 | 1.421262e-52 | 1.082119e-49 |
| MsG0380015350.01 | MsG0480018919.01 | 0.811423 | 7.052656e-51 | 4.364054e-48 |
| MsG0380015350.01 | MsG0480019635.01 | 0.808843 | 2.537520e-50 | 1.466310e-47 |
| MsG0380015350.01 | MsG0480019922.01 | 0.812219 | 4.732462e-51 | 2.991468e-48 |
| MsG0380015350.01 | MsG0480020495.01 | 0.809470 | 1.862607e-50 | 1.094508e-47 |
| MsG0380015350.01 | MsG0480020847.01 | 0.817320 | 3.505077e-52 | 2.544288e-49 |
| MsG0380015350.01 | MsG0480021450.01 | 0.806042 | 9.967189e-50 | 5.355612e-47 |
| MsG0380015350.01 | MsG0480022313.01 | 0.818178 | 2.244746e-52 | 1.668283e-49 |
| MsG0380015350.01 | MsG0480022325.01 | 0.810661 | 1.031681e-50 | 6.256086e-48 |
| MsG0380015350.01 | MsG0480022796.01 | 0.800906 | 1.156690e-48 | 5.450496e-46 |
| MsG0380015350.01 | MsG0480022982.01 | 0.828075 | 1.099810e-54 | 1.081680e-51 |
| MsG0380015350.01 | MsG0480022998.01 | 0.838915 | 2.165292e-57 | 2.942520e-54 |
| MsG0380015350.01 | MsG0480023262.01 | 0.843454 | 1.387751e-58 | 2.172423e-55 |
| MsG0380015350.01 | MsG0480023296.01 | 0.820358 | 7.153828e-53 | 5.647896e-50 |
| MsG0380015350.01 | MsG0480023653.01 | 0.803413 | 3.527282e-49 | 1.771416e-46 |
| MsG0380015350.01 | MsG0480023762.01 | 0.826349 | 2.848772e-54 | 2.664914e-51 |
| MsG0380015350.01 | MsG0580024388.01 | 0.831523 | 1.590847e-55 | 1.730752e-52 |
| MsG0380015350.01 | MsG0580024489.01 | 0.809246 | 2.079959e-50 | 1.215023e-47 |
| MsG0380015350.01 | MsG0580024758.01 | 0.829051 | 6.389884e-55 | 6.465257e-52 |
| MsG0380015350.01 | MsG0580024954.01 | 0.817451 | 3.275298e-52 | 2.386206e-49 |
| MsG0380015350.01 | MsG0580025124.01 | 0.824337 | 8.523587e-54 | 7.527672e-51 |
| MsG0380015350.01 | MsG0580025645.01 | 0.801692 | 7.988028e-49 | 3.839195e-46 |
| MsG0380015350.01 | MsG0580026145.01 | 0.808263 | 3.374236e-50 | 1.920588e-47 |
| MsG0380015350.01 | MsG0580028060.01 | 0.806994 | 6.275707e-50 | 3.455301e-47 |
| MsG0380015350.01 | MsG0580028756.01 | 0.822017 | 2.965631e-53 | 2.452444e-50 |
| MsG0380015350.01 | MsG0680031616.01 | 0.801122 | 1.045009e-48 | 4.951146e-46 |
| MsG0380015350.01 | MsG0680032499.01 | 0.810250 | 1.265641e-50 | 7.591488e-48 |
| MsG0380015350.01 | MsG0680034598.01 | 0.804472 | 2.125293e-49 | 1.096736e-46 |
| MsG0380015350.01 | MsG0780035954.01 | 0.819159 | 1.344193e-52 | 1.026376e-49 |
| MsG0380015350.01 | MsG0780036752.01 | 0.816535 | 5.259954e-52 | 3.736946e-49 |
| MsG0380015350.01 | MsG0780036941.01 | 0.842864 | 1.992503e-58 | 3.061391e-55 |
| MsG0380015350.01 | MsG0780038373.01 | 0.804396 | 2.204048e-49 | 1.135152e-46 |
| MsG0380015350.01 | MsG0780039189.01 | 0.869164 | 3.801103e-66 | 1.412490e-62 |
| MsG0380015350.01 | MsG0780040364.01 | 0.811210 | 7.846305e-51 | 4.827804e-48 |
| MsG0380015350.01 | MsG0780040365.01 | 0.804109 | 2.528737e-49 | 1.292875e-46 |
| MsG0380015350.01 | MsG0780040472.01 | 0.816268 | 6.035752e-52 | 4.257185e-49 |
| MsG0380015350.01 | MsG0780040482.01 | 0.814950 | 1.186437e-51 | 8.071333e-49 |
| MsG0380015350.01 | MsG0780040509.01 | 0.877254 | 7.280425e-69 | 3.636749e-65 |
| MsG0380015350.01 | MsG0780041063.01 | 0.856737 | 2.627276e-62 | 6.331604e-59 |
| MsG0380015350.01 | MsG0780041143.01 | 0.809213 | 2.114038e-50 | 1.233832e-47 |
| MsG0380015350.01 | MsG0780041579.01 | 0.803520 | 3.351999e-49 | 1.688076e-46 |
| MsG0380015350.01 | MsG0780041735.01 | 0.841452 | 4.712270e-58 | 6.927556e-55 |
| MsG0380015350.01 | MsG0880042773.01 | 0.831372 | 1.732651e-55 | 1.876578e-52 |
| MsG0380015350.01 | MsG0880042791.01 | 0.834095 | 3.653463e-56 | 4.291221e-53 |
| MsG0380015350.01 | MsG0880042906.01 | 0.800740 | 1.250804e-48 | 5.868816e-46 |
| MsG0380015350.01 | MsG0880043637.01 | 0.829449 | 5.115583e-55 | 5.237292e-52 |
| MsG0380015350.01 | MsG0880044540.01 | 0.801265 | 9.766917e-49 | 4.644104e-46 |
| MsG0380015350.01 | MsG0880044555.01 | 0.804472 | 2.124993e-49 | 1.096587e-46 |
| MsG0380015350.01 | MsG0880044782.01 | 0.818641 | 1.762863e-52 | 1.326925e-49 |
| MsG0380015350.01 | MsG0880045430.01 | 0.804367 | 2.234716e-49 | 1.150142e-46 |
| MsG0380015350.01 | MsG0880045890.01 | 0.811883 | 5.602954e-51 | 3.509205e-48 |
| MsG0380015350.01 | MsG0880046922.01 | 0.875489 | 2.958839e-68 | 1.383949e-64 |
| MsG0380015350.01 | MsG0880046991.01 | 0.826228 | 3.043119e-54 | 2.836952e-51 |
| MsG0380015350.01 | MsG0880047059.01 | 0.800703 | 1.272588e-48 | 5.965216e-46 |
| MsG0280007196.01 | MsG0380015350.01 | 0.822289 | 2.565468e-53 | 2.138255e-50 |
| MsG0280007359.01 | MsG0380015350.01 | 0.806811 | 6.860221e-50 | 3.759474e-47 |
| MsG0280009783.01 | MsG0380015350.01 | 0.814638 | 1.391714e-51 | 9.388949e-49 |
| MsG0280010015.01 | MsG0380015350.01 | 0.801563 | 8.486646e-49 | 4.065726e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015350.01.T01 | MTR_3g068065 | 78.182 | 275 | 11 | 2 | 1 | 226 | 1 | 275 | 5.07e-144 | 403 |
| MsG0380015350.01.T01 | MTR_3g068085 | 71.111 | 270 | 31 | 1 | 1 | 223 | 1 | 270 | 8.71e-128 | 362 |
| MsG0380015350.01.T01 | MTR_3g068070 | 68.939 | 264 | 35 | 1 | 1 | 217 | 1 | 264 | 6.49e-126 | 357 |
| MsG0380015350.01.T01 | MTR_3g068155 | 59.630 | 270 | 61 | 2 | 2 | 223 | 5 | 274 | 4.16e-107 | 310 |
| MsG0380015350.01.T01 | MTR_3g068175 | 59.926 | 272 | 60 | 3 | 1 | 223 | 1 | 272 | 8.10e-107 | 309 |
| MsG0380015350.01.T01 | MTR_3g068175 | 59.058 | 276 | 60 | 3 | 1 | 223 | 1 | 276 | 4.19e-106 | 307 |
| MsG0380015350.01.T01 | MTR_4g064883 | 49.248 | 266 | 88 | 1 | 4 | 222 | 3 | 268 | 2.16e-89 | 265 |
| MsG0380015350.01.T01 | MTR_4g064887 | 51.527 | 262 | 80 | 3 | 1 | 215 | 1 | 262 | 3.61e-86 | 256 |
| MsG0380015350.01.T01 | MTR_7g063270 | 50.193 | 259 | 79 | 2 | 6 | 214 | 7 | 265 | 2.99e-80 | 241 |
| MsG0380015350.01.T01 | MTR_7g063330 | 48.828 | 256 | 81 | 2 | 9 | 214 | 9 | 264 | 2.92e-79 | 238 |
| MsG0380015350.01.T01 | MTR_7g063320 | 49.020 | 255 | 79 | 3 | 9 | 212 | 9 | 263 | 3.59e-79 | 238 |
| MsG0380015350.01.T01 | MTR_7g063360 | 48.302 | 265 | 85 | 3 | 1 | 213 | 1 | 265 | 1.15e-78 | 237 |
| MsG0380015350.01.T01 | MTR_7g063260 | 48.077 | 260 | 84 | 2 | 6 | 214 | 7 | 266 | 2.46e-77 | 234 |
| MsG0380015350.01.T01 | MTR_7g063390 | 46.792 | 265 | 89 | 3 | 1 | 213 | 1 | 265 | 2.51e-77 | 234 |
| MsG0380015350.01.T01 | MTR_4g064887 | 60.870 | 161 | 63 | 0 | 1 | 161 | 1 | 161 | 4.64e-69 | 211 |
| MsG0380015350.01.T01 | MTR_4g064883 | 58.861 | 158 | 65 | 0 | 4 | 161 | 3 | 160 | 7.10e-66 | 203 |
| MsG0380015350.01.T01 | MTR_7g063270 | 51.376 | 218 | 89 | 3 | 6 | 214 | 7 | 216 | 3.71e-65 | 201 |
| MsG0380015350.01.T01 | MTR_3g068075 | 52.941 | 102 | 26 | 3 | 123 | 223 | 1 | 81 | 1.78e-22 | 87.8 |
| MsG0380015350.01.T01 | MTR_4g132850 | 27.823 | 248 | 123 | 7 | 15 | 212 | 7 | 248 | 1.53e-17 | 79.0 |
| MsG0380015350.01.T01 | MTR_5g098650 | 66.000 | 50 | 17 | 0 | 174 | 223 | 124 | 173 | 2.94e-14 | 68.6 |
| MsG0380015350.01.T01 | MTR_3g098470 | 32.653 | 147 | 97 | 2 | 8 | 154 | 25 | 169 | 1.25e-13 | 68.9 |
| MsG0380015350.01.T01 | MTR_8g044240 | 28.000 | 150 | 103 | 3 | 7 | 152 | 61 | 209 | 1.76e-11 | 63.2 |
| MsG0380015350.01.T01 | MTR_1563s0010 | 27.815 | 151 | 103 | 2 | 6 | 151 | 100 | 249 | 2.41e-11 | 62.8 |
| MsG0380015350.01.T01 | MTR_1g031180 | 31.293 | 147 | 99 | 2 | 8 | 154 | 30 | 174 | 5.45e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015350.01.T01 | AT5G06060 | 55.598 | 259 | 68 | 1 | 10 | 221 | 4 | 262 | 9.51e-96 | 280 |
| MsG0380015350.01.T01 | AT2G29150 | 50.758 | 264 | 83 | 2 | 1 | 217 | 1 | 264 | 8.57e-86 | 255 |
| MsG0380015350.01.T01 | AT2G29350 | 50.570 | 263 | 84 | 1 | 1 | 217 | 1 | 263 | 1.32e-84 | 252 |
| MsG0380015350.01.T01 | AT2G29290 | 50.579 | 259 | 79 | 2 | 10 | 219 | 2 | 260 | 1.18e-83 | 249 |
| MsG0380015350.01.T01 | AT2G29260 | 50.980 | 255 | 78 | 1 | 12 | 219 | 65 | 319 | 2.11e-83 | 251 |
| MsG0380015350.01.T01 | AT2G29360 | 49.811 | 265 | 85 | 2 | 1 | 217 | 1 | 265 | 7.82e-83 | 248 |
| MsG0380015350.01.T01 | AT1G07440 | 49.813 | 267 | 83 | 4 | 1 | 219 | 1 | 264 | 1.70e-82 | 247 |
| MsG0380015350.01.T01 | AT2G29340 | 48.659 | 261 | 85 | 2 | 10 | 221 | 2 | 262 | 5.65e-80 | 240 |
| MsG0380015350.01.T01 | AT2G29330 | 51.351 | 259 | 77 | 4 | 10 | 219 | 2 | 260 | 6.10e-80 | 240 |
| MsG0380015350.01.T01 | AT2G29340 | 49.035 | 259 | 83 | 2 | 10 | 219 | 2 | 260 | 1.89e-79 | 241 |
| MsG0380015350.01.T01 | AT2G30670 | 49.020 | 255 | 83 | 1 | 10 | 217 | 2 | 256 | 1.05e-78 | 237 |
| MsG0380015350.01.T01 | AT2G29370 | 47.925 | 265 | 90 | 2 | 1 | 217 | 1 | 265 | 6.58e-78 | 235 |
| MsG0380015350.01.T01 | AT1G07450 | 50.000 | 252 | 80 | 2 | 12 | 217 | 5 | 256 | 7.32e-78 | 235 |
| MsG0380015350.01.T01 | AT2G29320 | 49.805 | 257 | 79 | 3 | 13 | 219 | 11 | 267 | 7.34e-78 | 235 |
| MsG0380015350.01.T01 | AT2G29300 | 48.462 | 260 | 84 | 4 | 10 | 219 | 2 | 261 | 8.18e-76 | 230 |
| MsG0380015350.01.T01 | AT2G29300 | 48.413 | 252 | 82 | 3 | 10 | 213 | 2 | 253 | 3.71e-73 | 224 |
| MsG0380015350.01.T01 | AT2G29310 | 47.843 | 255 | 86 | 2 | 10 | 217 | 2 | 256 | 4.30e-73 | 223 |
| MsG0380015350.01.T01 | AT2G29290 | 48.945 | 237 | 72 | 2 | 32 | 219 | 7 | 243 | 1.22e-70 | 216 |
| MsG0380015350.01.T01 | AT2G29320 | 48.117 | 239 | 74 | 3 | 31 | 219 | 11 | 249 | 3.74e-67 | 207 |
| MsG0380015350.01.T01 | AT2G29350 | 47.768 | 224 | 71 | 1 | 40 | 217 | 2 | 225 | 6.29e-65 | 201 |
| MsG0380015350.01.T01 | AT2G29350 | 60.248 | 161 | 64 | 0 | 1 | 161 | 1 | 161 | 8.60e-65 | 201 |
| MsG0380015350.01.T01 | AT1G07440 | 58.125 | 160 | 64 | 1 | 1 | 160 | 1 | 157 | 1.21e-62 | 193 |
| MsG0380015350.01.T01 | AT2G29310 | 46.154 | 234 | 79 | 2 | 31 | 217 | 21 | 254 | 1.52e-60 | 191 |
| MsG0380015350.01.T01 | AT2G29340 | 57.792 | 154 | 65 | 0 | 10 | 163 | 2 | 155 | 6.02e-60 | 187 |
| MsG0380015350.01.T01 | AT2G29340 | 57.792 | 154 | 65 | 0 | 10 | 163 | 2 | 155 | 6.02e-60 | 187 |
| MsG0380015350.01.T01 | AT2G29320 | 59.732 | 149 | 60 | 0 | 13 | 161 | 11 | 159 | 1.13e-58 | 184 |
| MsG0380015350.01.T01 | AT2G29310 | 55.921 | 152 | 67 | 0 | 10 | 161 | 2 | 153 | 2.28e-55 | 176 |
| MsG0380015350.01.T01 | AT2G29310 | 54.198 | 131 | 60 | 0 | 31 | 161 | 21 | 151 | 8.04e-43 | 144 |
| MsG0380015350.01.T01 | AT2G29170 | 60.784 | 102 | 39 | 1 | 1 | 101 | 1 | 102 | 1.82e-36 | 124 |
| MsG0380015350.01.T01 | AT5G18210 | 26.482 | 253 | 132 | 6 | 14 | 212 | 7 | 259 | 1.75e-18 | 82.0 |
| MsG0380015350.01.T01 | AT4G05530 | 28.163 | 245 | 126 | 6 | 15 | 212 | 9 | 250 | 1.08e-15 | 74.3 |
| MsG0380015350.01.T01 | AT5G18210 | 25.296 | 253 | 122 | 7 | 14 | 212 | 7 | 246 | 5.22e-14 | 69.7 |
| MsG0380015350.01.T01 | AT1G10310 | 29.944 | 177 | 116 | 4 | 19 | 189 | 19 | 193 | 1.12e-12 | 65.5 |
| MsG0380015350.01.T01 | AT5G04900 | 30.435 | 138 | 92 | 3 | 18 | 152 | 80 | 216 | 1.34e-11 | 63.5 |
Find 51 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACAAGTTATTTCTGTTGA+TGG | 0.321935 | 3:-68901602 | MsG0380015350.01.T01:CDS |
| TATCCTCTTCTTAAAGAATC+TGG | 0.324082 | 3:-68902218 | MsG0380015350.01.T01:CDS |
| GTAGAGGAACTTGCTGAGTT+TGG | 0.369806 | 3:-68903124 | MsG0380015350.01.T01:CDS |
| GGAAGACACTTCATGTGTTT+CGG | 0.394739 | 3:+68901667 | None:intergenic |
| TCCGTTGTATACTCAATTGT+TGG | 0.398000 | 3:+68902302 | None:intergenic |
| CTATAGTGTACACTTGTTCT+AGG | 0.398286 | 3:-68903099 | MsG0380015350.01.T01:CDS |
| CTTCTTAAAGAATCTGGAAA+TGG | 0.409640 | 3:-68902212 | MsG0380015350.01.T01:CDS |
| AGAAATAGTCTTATGTTCTA+TGG | 0.413199 | 3:+68901435 | None:intergenic |
| CTCGTCACCGGCGGAACTCG+TGG | 0.413917 | 3:-68903163 | MsG0380015350.01.T01:CDS |
| GATATGTCAGTTGGCATAGA+TGG | 0.418094 | 3:+68902237 | None:intergenic |
| GATGGAGGATTTACTGCAAA+TGG | 0.433393 | 3:-68901584 | MsG0380015350.01.T01:CDS |
| AAAATTGATGAGTATTAACT+TGG | 0.439582 | 3:-68902270 | MsG0380015350.01.T01:CDS |
| ATCACTTCTTATGATCATGC+TGG | 0.440165 | 3:+68901553 | None:intergenic |
| CATGATGAGATGCTTGATAA+AGG | 0.466551 | 3:-68901358 | None:intergenic |
| TATCTCGTACACCGATAAAA+CGG | 0.472351 | 3:-68901694 | MsG0380015350.01.T01:CDS |
| TTGAATGAGTGGAAGGAGAA+AGG | 0.480371 | 3:-68903052 | MsG0380015350.01.T01:CDS |
| TGAGCTTAGGTAAACAATGT+TGG | 0.501292 | 3:-68902341 | MsG0380015350.01.T01:intron |
| TTTCCAGATTCTTTAAGAAG+AGG | 0.503369 | 3:+68902215 | None:intergenic |
| ACCGGCGGAACTCGTGGGAT+CGG | 0.517357 | 3:-68903157 | MsG0380015350.01.T01:CDS |
| AATAACTTGTCCAGTGATGT+AGG | 0.517499 | 3:+68901613 | None:intergenic |
| TTCTCAGTGTTAGCAAGATA+AGG | 0.518622 | 3:-68903260 | MsG0380015350.01.T01:five_prime_UTR |
| CCAGCTGCTTCCTACATCAC+TGG | 0.520639 | 3:-68901623 | MsG0380015350.01.T01:CDS |
| TCACTTCTTATGATCATGCT+GGG | 0.528359 | 3:+68901554 | None:intergenic |
| AACACATGAAGTGTCTTCCT+TGG | 0.534095 | 3:-68901663 | MsG0380015350.01.T01:CDS |
| ACCGATCCCACGAGTTCCGC+CGG | 0.539004 | 3:+68903156 | None:intergenic |
| TAAGAAGAGGATATGTCAGT+TGG | 0.546406 | 3:+68902228 | None:intergenic |
| CAAGTTATTTCTGTTGATGG+AGG | 0.549415 | 3:-68901599 | MsG0380015350.01.T01:CDS |
| GGATTCACAGCTCTCGTCAC+CGG | 0.550668 | 3:-68903175 | MsG0380015350.01.T01:CDS |
| TCTCCTTGTTAATCATCTGA+AGG | 0.552677 | 3:-68901391 | MsG0380015350.01.T01:three_prime_UTR |
| GACTTCTTCTTCTCAAAGAG+AGG | 0.564516 | 3:-68902999 | MsG0380015350.01.T01:CDS |
| AAGAGAGGAGCTTGTTCGAA+AGG | 0.571197 | 3:-68902984 | MsG0380015350.01.T01:CDS |
| AAACTCAAGATGGTCCCTCA+AGG | 0.573784 | 3:-68903197 | MsG0380015350.01.T01:CDS |
| TCGTCACCGGCGGAACTCGT+GGG | 0.574228 | 3:-68903162 | MsG0380015350.01.T01:CDS |
| GAGAGCTGTGAATCCCTTGA+GGG | 0.579284 | 3:+68903183 | None:intergenic |
| AGAGGAGCTTGTTCGAAAGG+TGG | 0.587374 | 3:-68902981 | MsG0380015350.01.T01:CDS |
| CCAGTGATGTAGGAAGCAGC+TGG | 0.588298 | 3:+68901623 | None:intergenic |
| AACTCAAGATGGTCCCTCAA+GGG | 0.601552 | 3:-68903196 | MsG0380015350.01.T01:CDS |
| GATGATTAACAAGGAGACAC+AGG | 0.610429 | 3:+68901397 | None:intergenic |
| AGGAAGCAGCTGGCAGACAA+AGG | 0.611292 | 3:+68901633 | None:intergenic |
| CGAGAGCTGTGAATCCCTTG+AGG | 0.617629 | 3:+68903182 | None:intergenic |
| CAGACAAAGGAAAGCTACCA+AGG | 0.625310 | 3:+68901646 | None:intergenic |
| TTCACAGCTCTCGTCACCGG+CGG | 0.631222 | 3:-68903172 | MsG0380015350.01.T01:CDS |
| TGAATGAACGCTTGAATGAG+TGG | 0.642583 | 3:-68903063 | MsG0380015350.01.T01:CDS |
| TGAACGCTTGAATGAGTGGA+AGG | 0.643639 | 3:-68903059 | MsG0380015350.01.T01:CDS |
| AGACCTTCAGATGATTAACA+AGG | 0.652445 | 3:+68901388 | None:intergenic |
| CACTTGTTCTAGGAATGAAG+AGG | 0.665255 | 3:-68903089 | MsG0380015350.01.T01:CDS |
| ACAGTAGCAGAAACTCAAGA+TGG | 0.666153 | 3:-68903207 | MsG0380015350.01.T01:CDS |
| ACCAACAATTGAGTATACAA+CGG | 0.674590 | 3:-68902303 | MsG0380015350.01.T01:CDS |
| ACAATGTTGGAACAAATGTG+AGG | 0.690959 | 3:-68902328 | MsG0380015350.01.T01:CDS |
| GTTTGTCAGAGATGCAACAG+AGG | 0.693930 | 3:+68902172 | None:intergenic |
| GATCGGTCATGCTATCGTAG+AGG | 0.705413 | 3:-68903140 | MsG0380015350.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGATGAGTATTAACT+TGG | - | Chr3:68902372-68902391 | MsG0380015350.01.T01:intron | 20.0% |
| !! | AAGCATAAGAAAATCTTAAA+TGG | + | Chr3:68902104-68902123 | None:intergenic | 20.0% |
| !! | ACTTATTGTAGAAAATTTAG+TGG | - | Chr3:68901846-68901865 | MsG0380015350.01.T01:intron | 20.0% |
| !!! | CATTTTTTCTTTATGAAGAT+TGG | - | Chr3:68902784-68902803 | MsG0380015350.01.T01:intron | 20.0% |
| !!! | TGTACTGATATAATTTGATT+TGG | - | Chr3:68902814-68902833 | MsG0380015350.01.T01:intron | 20.0% |
| ! | AACATTACATAATCGTTAAG+TGG | + | Chr3:68902727-68902746 | None:intergenic | 25.0% |
| ! | TAAGGTAACAAAAACAACAA+TGG | - | Chr3:68901400-68901419 | MsG0380015350.01.T01:three_prime_UTR | 25.0% |
| ! | TCTAATATTCTTATGAGCTT+AGG | - | Chr3:68902288-68902307 | MsG0380015350.01.T01:CDS | 25.0% |
| ! | TTAAGAACATTGCATTACAT+AGG | - | Chr3:68901965-68901984 | MsG0380015350.01.T01:intron | 25.0% |
| ! | TTAGCATATAACTGTAAGAA+AGG | + | Chr3:68902905-68902924 | None:intergenic | 25.0% |
| !!! | AGAAATAGTCTTATGTTCTA+TGG | + | Chr3:68903210-68903229 | None:intergenic | 25.0% |
| AAAAAGTCTTGCTTGTGAAT+GGG | - | Chr3:68902651-68902670 | MsG0380015350.01.T01:intron | 30.0% | |
| AACATTGCATTACATAGGTA+GGG | - | Chr3:68901970-68901989 | MsG0380015350.01.T01:intron | 30.0% | |
| AATGGGCAAAAGACAATATA+AGG | - | Chr3:68902668-68902687 | MsG0380015350.01.T01:intron | 30.0% | |
| ACCAACAATTGAGTATACAA+CGG | - | Chr3:68902339-68902358 | MsG0380015350.01.T01:intron | 30.0% | |
| CTTCTTAAAGAATCTGGAAA+TGG | - | Chr3:68902430-68902449 | MsG0380015350.01.T01:intron | 30.0% | |
| GTTTACACATGATTGGAATT+TGG | + | Chr3:68901904-68901923 | None:intergenic | 30.0% | |
| TATCCTCTTCTTAAAGAATC+TGG | - | Chr3:68902424-68902443 | MsG0380015350.01.T01:intron | 30.0% | |
| ! | TTTCCAGATTCTTTAAGAAG+AGG | + | Chr3:68902430-68902449 | None:intergenic | 30.0% |
| !! | AAAATGATAAGAGCTCTGAA+TGG | + | Chr3:68902770-68902789 | None:intergenic | 30.0% |
| !! | AGCTCTGAATGGATAATTAA+AGG | + | Chr3:68902759-68902778 | None:intergenic | 30.0% |
| ACAATGTTGGAACAAATGTG+AGG | - | Chr3:68902314-68902333 | MsG0380015350.01.T01:CDS | 35.0% | |
| AGACCTTCAGATGATTAACA+AGG | + | Chr3:68903257-68903276 | None:intergenic | 35.0% | |
| ATCACTTCTTATGATCATGC+TGG | + | Chr3:68903092-68903111 | None:intergenic | 35.0% | |
| ATGACTCGTTTACACATGAT+TGG | + | Chr3:68901911-68901930 | None:intergenic | 35.0% | |
| CAAAAAGTCTTGCTTGTGAA+TGG | - | Chr3:68902650-68902669 | MsG0380015350.01.T01:intron | 35.0% | |
| CTATAGTGTACACTTGTTCT+AGG | - | Chr3:68901543-68901562 | MsG0380015350.01.T01:exon | 35.0% | |
| GAACATTGCATTACATAGGT+AGG | - | Chr3:68901969-68901988 | MsG0380015350.01.T01:intron | 35.0% | |
| GACATGAATAAGTGGAAATC+CGG | + | Chr3:68902011-68902030 | None:intergenic | 35.0% | |
| TAAGAAGAGGATATGTCAGT+TGG | + | Chr3:68902417-68902436 | None:intergenic | 35.0% | |
| TATCTCGTACACCGATAAAA+CGG | - | Chr3:68902948-68902967 | MsG0380015350.01.T01:intron | 35.0% | |
| TCACTTCTTATGATCATGCT+GGG | + | Chr3:68903091-68903110 | None:intergenic | 35.0% | |
| TCCGTTGTATACTCAATTGT+TGG | + | Chr3:68902343-68902362 | None:intergenic | 35.0% | |
| TCTCCTTGTTAATCATCTGA+AGG | - | Chr3:68903251-68903270 | MsG0380015350.01.T01:five_prime_UTR | 35.0% | |
| TGAGCTTAGGTAAACAATGT+TGG | - | Chr3:68902301-68902320 | MsG0380015350.01.T01:CDS | 35.0% | |
| TTCTCAGTGTTAGCAAGATA+AGG | - | Chr3:68901382-68901401 | MsG0380015350.01.T01:three_prime_UTR | 35.0% | |
| ! | AAGGTGGCTTCTGATTTTAA+TGG | - | Chr3:68901677-68901696 | MsG0380015350.01.T01:CDS | 35.0% |
| ! | AATAACTTGTCCAGTGATGT+AGG | + | Chr3:68903032-68903051 | None:intergenic | 35.0% |
| ! | GAGAAAGGTTTTTCTGTTCA+TGG | - | Chr3:68901605-68901624 | MsG0380015350.01.T01:CDS | 35.0% |
| !! | AGAACGTGTATGTGTTTTTG+TGG | + | Chr3:68901366-68901385 | None:intergenic | 35.0% |
| !! | CAAGTTATTTCTGTTGATGG+AGG | - | Chr3:68903043-68903062 | MsG0380015350.01.T01:CDS | 35.0% |
| !! | GGACAAGTTATTTCTGTTGA+TGG | - | Chr3:68903040-68903059 | MsG0380015350.01.T01:CDS | 35.0% |
| ACAGTAGCAGAAACTCAAGA+TGG | - | Chr3:68901435-68901454 | MsG0380015350.01.T01:three_prime_UTR | 40.0% | |
| CATTACATAGGTAGGGACTA+GGG | - | Chr3:68901977-68901996 | MsG0380015350.01.T01:intron | 40.0% | |
| GACTTCTTCTTCTCAAAGAG+AGG | - | Chr3:68901643-68901662 | MsG0380015350.01.T01:CDS | 40.0% | |
| GATATGTCAGTTGGCATAGA+TGG | + | Chr3:68902408-68902427 | None:intergenic | 40.0% | |
| GATGATTAACAAGGAGACAC+AGG | + | Chr3:68903248-68903267 | None:intergenic | 40.0% | |
| GATGGAGGATTTACTGCAAA+TGG | - | Chr3:68903058-68903077 | MsG0380015350.01.T01:CDS | 40.0% | |
| GGAAGACACTTCATGTGTTT+CGG | + | Chr3:68902978-68902997 | None:intergenic | 40.0% | |
| TGAATGAACGCTTGAATGAG+TGG | - | Chr3:68901579-68901598 | MsG0380015350.01.T01:CDS | 40.0% | |
| TGCGACAAGACATGAATAAG+TGG | + | Chr3:68902019-68902038 | None:intergenic | 40.0% | |
| TTGAATGAGTGGAAGGAGAA+AGG | - | Chr3:68901590-68901609 | MsG0380015350.01.T01:CDS | 40.0% | |
| ! | AACACATGAAGTGTCTTCCT+TGG | - | Chr3:68902979-68902998 | MsG0380015350.01.T01:CDS | 40.0% |
| ! | AGTGGCGTTTTTGTATACCA+CGG | + | Chr3:68902709-68902728 | None:intergenic | 40.0% |
| ! | GTTTCGGCTATCCGTTTTAT+CGG | + | Chr3:68902962-68902981 | None:intergenic | 40.0% |
| !! | ATCGCATTTTCAAGCTTCTG+AGG | + | Chr3:68902138-68902157 | None:intergenic | 40.0% |
| !! | CACTTGTTCTAGGAATGAAG+AGG | - | Chr3:68901553-68901572 | MsG0380015350.01.T01:CDS | 40.0% |
| !! | TCGCATTTTCAAGCTTCTGA+GGG | + | Chr3:68902137-68902156 | None:intergenic | 40.0% |
| AAACTCAAGATGGTCCCTCA+AGG | - | Chr3:68901445-68901464 | MsG0380015350.01.T01:three_prime_UTR | 45.0% | |
| AACTCAAGATGGTCCCTCAA+GGG | - | Chr3:68901446-68901465 | MsG0380015350.01.T01:three_prime_UTR | 45.0% | |
| ACCATATACGCAGCGTGTAA+AGG | - | Chr3:68902505-68902524 | MsG0380015350.01.T01:intron | 45.0% | |
| ACCTTTACACGCTGCGTATA+TGG | + | Chr3:68902509-68902528 | None:intergenic | 45.0% | |
| CAGACAAAGGAAAGCTACCA+AGG | + | Chr3:68902999-68903018 | None:intergenic | 45.0% | |
| GCATTACATAGGTAGGGACT+AGG | - | Chr3:68901976-68901995 | MsG0380015350.01.T01:intron | 45.0% | |
| GTTGCATCTCTGACAAACGT+AGG | - | Chr3:68902475-68902494 | MsG0380015350.01.T01:intron | 45.0% | |
| GTTTGTCAGAGATGCAACAG+AGG | + | Chr3:68902473-68902492 | None:intergenic | 45.0% | |
| TCTCTGACAAACGTAGGTTC+TGG | - | Chr3:68902481-68902500 | MsG0380015350.01.T01:intron | 45.0% | |
| TGAACGCTTGAATGAGTGGA+AGG | - | Chr3:68901583-68901602 | MsG0380015350.01.T01:CDS | 45.0% | |
| TGCAGCTGAAGAAAACAGCA+CGG | + | Chr3:68902617-68902636 | None:intergenic | 45.0% | |
| ! | AAGAGAGGAGCTTGTTCGAA+AGG | - | Chr3:68901658-68901677 | MsG0380015350.01.T01:CDS | 45.0% |
| !! | GTAGAGGAACTTGCTGAGTT+TGG | - | Chr3:68901518-68901537 | MsG0380015350.01.T01:three_prime_UTR | 45.0% |
| GAGAGCTGTGAATCCCTTGA+GGG | + | Chr3:68901462-68901481 | None:intergenic | 50.0% | |
| GATCGGTCATGCTATCGTAG+AGG | - | Chr3:68901502-68901521 | MsG0380015350.01.T01:three_prime_UTR | 50.0% | |
| TGAGCTGAAGTCATGCGTGA+CGG | + | Chr3:68902244-68902263 | None:intergenic | 50.0% | |
| ! | AGAGGAGCTTGTTCGAAAGG+TGG | - | Chr3:68901661-68901680 | MsG0380015350.01.T01:CDS | 50.0% |
| AGGAAGCAGCTGGCAGACAA+AGG | + | Chr3:68903012-68903031 | None:intergenic | 55.0% | |
| CCAGCTGCTTCCTACATCAC+TGG | - | Chr3:68903019-68903038 | MsG0380015350.01.T01:CDS | 55.0% | |
| CCAGTGATGTAGGAAGCAGC+TGG | + | Chr3:68903022-68903041 | None:intergenic | 55.0% | |
| CGAGAGCTGTGAATCCCTTG+AGG | + | Chr3:68901463-68901482 | None:intergenic | 55.0% | |
| GGATTCACAGCTCTCGTCAC+CGG | - | Chr3:68901467-68901486 | MsG0380015350.01.T01:three_prime_UTR | 55.0% | |
| ! | AGGGACTAGGGTTCTAACGC+CGG | - | Chr3:68901989-68902008 | MsG0380015350.01.T01:intron | 55.0% |
| GGAGCAACTGTGTTGCACCG+TGG | - | Chr3:68902689-68902708 | MsG0380015350.01.T01:intron | 60.0% | |
| TTCACAGCTCTCGTCACCGG+CGG | - | Chr3:68901470-68901489 | MsG0380015350.01.T01:three_prime_UTR | 60.0% | |
| ACCGATCCCACGAGTTCCGC+CGG | + | Chr3:68901489-68901508 | None:intergenic | 65.0% | |
| ACCGGCGGAACTCGTGGGAT+CGG | - | Chr3:68901485-68901504 | MsG0380015350.01.T01:three_prime_UTR | 65.0% | |
| TCGTCACCGGCGGAACTCGT+GGG | - | Chr3:68901480-68901499 | MsG0380015350.01.T01:three_prime_UTR | 65.0% | |
| CTCGTCACCGGCGGAACTCG+TGG | - | Chr3:68901479-68901498 | MsG0380015350.01.T01:three_prime_UTR | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 68901359 | 68903305 | 68901359 | ID=MsG0380015350.01;Name=MsG0380015350.01 |
| Chr3 | mRNA | 68901359 | 68903305 | 68901359 | ID=MsG0380015350.01.T01;Parent=MsG0380015350.01;Name=MsG0380015350.01.T01;_AED=0.44;_eAED=0.44;_QI=60|0.5|0.66|1|1|1|3|191|226 |
| Chr3 | exon | 68901359 | 68901750 | 68901359 | ID=MsG0380015350.01.T01:exon:286;Parent=MsG0380015350.01.T01 |
| Chr3 | exon | 68902172 | 68902354 | 68902172 | ID=MsG0380015350.01.T01:exon:285;Parent=MsG0380015350.01.T01 |
| Chr3 | exon | 68902949 | 68903305 | 68902949 | ID=MsG0380015350.01.T01:exon:284;Parent=MsG0380015350.01.T01 |
| Chr3 | five_prime_UTR | 68903246 | 68903305 | 68903246 | ID=MsG0380015350.01.T01:five_prime_utr;Parent=MsG0380015350.01.T01 |
| Chr3 | CDS | 68902949 | 68903245 | 68902949 | ID=MsG0380015350.01.T01:cds;Parent=MsG0380015350.01.T01 |
| Chr3 | CDS | 68902172 | 68902354 | 68902172 | ID=MsG0380015350.01.T01:cds;Parent=MsG0380015350.01.T01 |
| Chr3 | CDS | 68901550 | 68901750 | 68901550 | ID=MsG0380015350.01.T01:cds;Parent=MsG0380015350.01.T01 |
| Chr3 | three_prime_UTR | 68901359 | 68901549 | 68901359 | ID=MsG0380015350.01.T01:three_prime_utr;Parent=MsG0380015350.01.T01 |
| Gene Sequence |
| Protein sequence |
