AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024754.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024754.01.T01 MTR_5g015850 81.988 161 2 2 54 213 3 137 2.22e-88 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024754.01.T01 AT1G07830 68.148 135 15 2 81 213 36 144 2.88e-59 183

Find 51 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGCCCTGTGAGCTTTC+TGG 0.163520 5:-9240099 MsG0580024754.01.T01:CDS
ATGGTCTTTGATGACTTAAA+AGG 0.289344 5:-9238930 MsG0580024754.01.T01:exon
CTGAATTGCGTCTGAAATCT+TGG 0.295639 5:-9239404 MsG0580024754.01.T01:CDS
TTTCATTATAGCCACACATT+AGG 0.377742 5:-9239507 MsG0580024754.01.T01:intron
TGGCATTGCAGCCAATGTTT+TGG 0.380082 5:-9239721 MsG0580024754.01.T01:intron
TGAGACTGTTAAAGAAATAA+AGG 0.381017 5:-9238687 MsG0580024754.01.T01:three_prime_UTR
CCCTCTTCCGGAGTTCTTTG+AGG 0.397007 5:-9239619 MsG0580024754.01.T01:CDS
GAGATGAAGAAAATGATAAA+TGG 0.401349 5:-9238949 MsG0580024754.01.T01:CDS
TTAGGTAAGCGCTCCGGGTT+AGG 0.401390 5:+9239288 None:intergenic
GTTGTGGTATGTGTTGTTAA+AGG 0.420053 5:-9239367 MsG0580024754.01.T01:CDS
AACGAGACACCAGTTGTCTA+TGG 0.421926 5:-9239585 MsG0580024754.01.T01:CDS
TGAATTGCGTCTGAAATCTT+GGG 0.436291 5:-9239403 MsG0580024754.01.T01:CDS
AGATAGGACATCTACTTTCA+TGG 0.439571 5:-9238874 MsG0580024754.01.T01:three_prime_UTR
TGCTGTTAGAAACCCTCTTC+CGG 0.440920 5:-9239631 MsG0580024754.01.T01:CDS
ACAATGGTTAAGTGACTCTC+AGG 0.460628 5:+9238759 None:intergenic
TCAGCAGACCTCCTTGCGTC+TGG 0.463615 5:+9238970 None:intergenic
ATACCTTAGGTAAGCGCTCC+GGG 0.470522 5:+9239283 None:intergenic
GAACTTGCGCTTTCCTAACC+CGG 0.481233 5:-9239301 MsG0580024754.01.T01:CDS
GAATCTCAATGGTGTTGAAA+AGG 0.482711 5:-9241515 MsG0580024754.01.T01:CDS
GCCTCAAAGAACTCCGGAAG+AGG 0.493349 5:+9239618 None:intergenic
TATTTCATACCATAGACAAC+TGG 0.503485 5:+9239576 None:intergenic
AGTCTTTGTATTGAAGATGT+TGG 0.506311 5:-9241541 MsG0580024754.01.T01:CDS
CTCGTGAACATTCTGCAGAT+GGG 0.512970 5:+9238724 None:intergenic
AATACCTTAGGTAAGCGCTC+CGG 0.514447 5:+9239282 None:intergenic
CCTATTCTATTGAAAAGAAG+AGG 0.520689 5:+9239549 None:intergenic
AATCATGTATTTGAACAGAC+TGG 0.525051 5:+9238828 None:intergenic
GCTCGTGAACATTCTGCAGA+TGG 0.538875 5:+9238723 None:intergenic
CTCATGTGCTAGAGCTGAGT+GGG 0.540562 5:-9240070 MsG0580024754.01.T01:CDS
AAACAGTATTCCACTACAGC+TGG 0.543067 5:-9239654 MsG0580024754.01.T01:CDS
TATTTCTTTAACAGTCTCAC+AGG 0.543526 5:+9238691 None:intergenic
ACACATACCACAACTTATGA+AGG 0.545630 5:+9239376 None:intergenic
TTTGTTAGGTCGGAGTTGGA+AGG 0.551769 5:-9239430 MsG0580024754.01.T01:intron
TGTATTTGAACAGACTGGCT+TGG 0.552486 5:+9238833 None:intergenic
GGGATGACCTTCATAAGTTG+TGG 0.556409 5:-9239383 MsG0580024754.01.T01:CDS
TCTAACAGCACCAGCTGTAG+TGG 0.557909 5:+9239644 None:intergenic
AAATGCTCTTGCCAAAACAT+TGG 0.562629 5:+9239710 None:intergenic
TAACCCGGAGCGCTTACCTA+AGG 0.570629 5:-9239286 MsG0580024754.01.T01:intron
GTGAAAGCCAGAAAGCTCAC+AGG 0.578022 5:+9240092 None:intergenic
CTGTCTGCCTCAAAGAACTC+CGG 0.578032 5:+9239612 None:intergenic
TGTGCTAGAGCTGAGTGGGA+TGG 0.582426 5:-9240066 MsG0580024754.01.T01:intron
TGGGGTTCATTTGACGACAA+TGG 0.593531 5:+9238743 None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG 0.599992 5:+9239619 None:intergenic
CGATTGAAGAACCAGACGCA+AGG 0.611981 5:-9238981 MsG0580024754.01.T01:CDS
GACTGTTAAAGAAATAAAGG+TGG 0.634963 5:-9238684 MsG0580024754.01.T01:three_prime_UTR
TCGTGAACATTCTGCAGATG+GGG 0.641508 5:+9238725 None:intergenic
GATGTTGGCAAGAATCTCAA+TGG 0.642465 5:-9241526 MsG0580024754.01.T01:CDS
CAGTAAGCACATGCTTGATG+CGG 0.642610 5:+9239010 None:intergenic
TGAAAGCCAGAAAGCTCACA+GGG 0.645070 5:+9240093 None:intergenic
ACTCATGTGCTAGAGCTGAG+TGG 0.658404 5:-9240071 MsG0580024754.01.T01:CDS
TTGAAGAACCAGACGCAAGG+AGG 0.691763 5:-9238978 MsG0580024754.01.T01:CDS
CAATGGTTAAGTGACTCTCA+GGG 0.702841 5:+9238760 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATGTTAAATTTCGTAT+GGG - Chr5:9241142-9241161 MsG0580024754.01.T01:intron 15.0%
!!! TTATCATTTTTTTTTTGACA+AGG - Chr5:9239576-9239595 MsG0580024754.01.T01:CDS 15.0%
!!! TTATTTTATTTCAGTAACAT+TGG - Chr5:9240449-9240468 MsG0580024754.01.T01:intron 15.0%
!! AACTAGTTAAGTATTATATC+AGG + Chr5:9240024-9240043 None:intergenic 20.0%
!! ATAATGATCAATTACATTCA+TGG + Chr5:9239817-9239836 None:intergenic 20.0%
!! GTAAAATGTTAAATTTCGTA+TGG - Chr5:9241141-9241160 MsG0580024754.01.T01:intron 20.0%
!! GTTATAAAGTAATGAAACTT+AGG - Chr5:9240041-9240060 MsG0580024754.01.T01:intron 20.0%
!! TACTCATATTTAATCATCTA+CGG + Chr5:9239154-9239173 None:intergenic 20.0%
!!! AAACAAGTTCATTTTTCTTT+TGG - Chr5:9239270-9239289 MsG0580024754.01.T01:intron 20.0%
!!! AAATTGAAGCATTTTTCAAA+TGG - Chr5:9239117-9239136 MsG0580024754.01.T01:intron 20.0%
!!! AACTTTTGAATTTTCTGAAT+AGG - Chr5:9239878-9239897 MsG0580024754.01.T01:intron 20.0%
!!! ATAGTTTTACCATTTTTGTT+AGG - Chr5:9240809-9240828 MsG0580024754.01.T01:intron 20.0%
!!! GATTTAATTTATGTGAAATC+AGG - Chr5:9241204-9241223 MsG0580024754.01.T01:intron 20.0%
!!! TTTTGTAAAGAATTGTAGAA+AGG - Chr5:9239624-9239643 MsG0580024754.01.T01:CDS 20.0%
! AGTAAAACACTAGTAGAAAA+AGG + Chr5:9239907-9239926 None:intergenic 25.0%
! CATCATTAACTAACTTATTC+CGG + Chr5:9239680-9239699 None:intergenic 25.0%
! CTAATGATGTATTATTGCAT+AGG + Chr5:9240400-9240419 None:intergenic 25.0%
! GAGATGAAGAAAATGATAAA+TGG - Chr5:9241304-9241323 MsG0580024754.01.T01:intron 25.0%
! TAAAAGTGAAGAAATACCTT+AGG + Chr5:9240986-9241005 None:intergenic 25.0%
! TATAAGAAAACAAAGTGATG+AGG + Chr5:9240792-9240811 None:intergenic 25.0%
! TATAGTCACAGATTTAGTAT+TGG + Chr5:9240217-9240236 None:intergenic 25.0%
! TATCTATTAACTAGACTAGA+TGG + Chr5:9239512-9239531 None:intergenic 25.0%
! TCACAAAAGTTGTTCAAAAA+TGG + Chr5:9238897-9238916 None:intergenic 25.0%
! TCGAATATTCATTAGTAATG+TGG + Chr5:9239732-9239751 None:intergenic 25.0%
! TGAGACTGTTAAAGAAATAA+AGG - Chr5:9241566-9241585 MsG0580024754.01.T01:CDS 25.0%
! TGTATTCTAATCAATCTGAT+TGG - Chr5:9240512-9240531 MsG0580024754.01.T01:intron 25.0%
!! AAATTCAAAAGTTGATTGTG+AGG + Chr5:9239871-9239890 None:intergenic 25.0%
!! ATATGTTTTCGTTGCATTTT+TGG - Chr5:9241346-9241365 MsG0580024754.01.T01:intron 25.0%
!! ATGCAAAAACTTTGAAAGAA+AGG - Chr5:9239424-9239443 MsG0580024754.01.T01:CDS 25.0%
!! GTCACAAAGTTGTTTAAAAT+TGG - Chr5:9239013-9239032 MsG0580024754.01.T01:CDS 25.0%
!! TATGTTTTCGTTGCATTTTT+GGG - Chr5:9241347-9241366 MsG0580024754.01.T01:intron 25.0%
!!! ATTTTTTTGTACCTAATGTG+TGG + Chr5:9240760-9240779 None:intergenic 25.0%
!!! TCATTTTTCTTTTGGCAAAA+GGG - Chr5:9239278-9239297 MsG0580024754.01.T01:intron 25.0%
!!! TTCATTTTTCTTTTGGCAAA+AGG - Chr5:9239277-9239296 MsG0580024754.01.T01:intron 25.0%
!!! TTTTACCATTTTTGTTAGGT+CGG - Chr5:9240813-9240832 MsG0580024754.01.T01:intron 25.0%
AAAACACTAGTAGAAAAAGG+CGG + Chr5:9239904-9239923 None:intergenic 30.0%
AATCATGTATTTGAACAGAC+TGG + Chr5:9241428-9241447 None:intergenic 30.0%
AGTAATGAAACTTAGGTGAT+TGG - Chr5:9240048-9240067 MsG0580024754.01.T01:intron 30.0%
AGTCTTTGTATTGAAGATGT+TGG - Chr5:9238712-9238731 MsG0580024754.01.T01:three_prime_UTR 30.0%
AGTTATGTGGACATTGATAT+AGG - Chr5:9241045-9241064 MsG0580024754.01.T01:intron 30.0%
ATCAATGTCCACATAACTAA+GGG + Chr5:9241043-9241062 None:intergenic 30.0%
ATCTTGCTCAAAATATTGGA+AGG + Chr5:9239222-9239241 None:intergenic 30.0%
ATGTTAAATTTCGTATGGGT+TGG - Chr5:9241146-9241165 MsG0580024754.01.T01:intron 30.0%
CACAAGTCAATTACTATTAG+AGG + Chr5:9239055-9239074 None:intergenic 30.0%
CATATTTAATCATCTACGGT+CGG + Chr5:9239150-9239169 None:intergenic 30.0%
CCTATTCTATTGAAAAGAAG+AGG + Chr5:9240707-9240726 None:intergenic 30.0%
GACTGTTAAAGAAATAAAGG+TGG - Chr5:9241569-9241588 MsG0580024754.01.T01:CDS 30.0%
GTCAATTACTATTAGAGGAA+TGG + Chr5:9239050-9239069 None:intergenic 30.0%
GTGTAAAAGAATATGAACAG+CGG - Chr5:9240265-9240284 MsG0580024754.01.T01:intron 30.0%
TAGTTGTCATCTCAAGAAAT+AGG - Chr5:9239991-9240010 MsG0580024754.01.T01:intron 30.0%
TATCAATGTCCACATAACTA+AGG + Chr5:9241044-9241063 None:intergenic 30.0%
TATTTCATACCATAGACAAC+TGG + Chr5:9240680-9240699 None:intergenic 30.0%
TATTTCTTTAACAGTCTCAC+AGG + Chr5:9241565-9241584 None:intergenic 30.0%
TGAAACTCTAAACTTGAGAA+AGG + Chr5:9240097-9240116 None:intergenic 30.0%
TGATCAATTACATTCATGGT+TGG + Chr5:9239813-9239832 None:intergenic 30.0%
TGTTAAATTTCGTATGGGTT+GGG - Chr5:9241147-9241166 MsG0580024754.01.T01:intron 30.0%
TTTCATTATAGCCACACATT+AGG - Chr5:9240746-9240765 MsG0580024754.01.T01:intron 30.0%
! ATGGTCTTTGATGACTTAAA+AGG - Chr5:9241323-9241342 MsG0580024754.01.T01:intron 30.0%
! CCTCTTCTTTTCAATAGAAT+AGG - Chr5:9240704-9240723 MsG0580024754.01.T01:intron 30.0%
! GAAAAATAGAGCATCATTCT+TGG - Chr5:9238805-9238824 MsG0580024754.01.T01:three_prime_UTR 30.0%
! TGCAAATAACTAGCTCATTT+TGG - Chr5:9239838-9239857 MsG0580024754.01.T01:intron 30.0%
! TTTTGTTACCCTTAGTTATG+TGG - Chr5:9241032-9241051 MsG0580024754.01.T01:intron 30.0%
!! TATTTCTTCACTTTTACACC+TGG - Chr5:9240990-9241009 MsG0580024754.01.T01:intron 30.0%
!! TGGCAGTTTGTGTTTTAAAT+TGG - Chr5:9239399-9239418 MsG0580024754.01.T01:CDS 30.0%
AAATGCTCTTGCCAAAACAT+TGG + Chr5:9240546-9240565 None:intergenic 35.0%
ACACATACCACAACTTATGA+AGG + Chr5:9240880-9240899 None:intergenic 35.0%
ACCAAAAACTCTAATGACAG+AGG + Chr5:9240291-9240310 None:intergenic 35.0%
AGACAATTTCTTATACCCAG+AGG - Chr5:9239538-9239557 MsG0580024754.01.T01:CDS 35.0%
AGATAGGACATCTACTTTCA+TGG - Chr5:9241379-9241398 MsG0580024754.01.T01:intron 35.0%
ATACCAATAGAGAGAGAAAG+AGG + Chr5:9238965-9238984 None:intergenic 35.0%
ATTTAGAAGCCAGAGAACAA+TGG + Chr5:9239248-9239267 None:intergenic 35.0%
CATGACATCTATAGTTAGGT+TGG - Chr5:9239379-9239398 MsG0580024754.01.T01:CDS 35.0%
CCAATAAGAAGAGAGGAAAA+AGG - Chr5:9238988-9239007 MsG0580024754.01.T01:CDS 35.0%
CTGACATGACATCTATAGTT+AGG - Chr5:9239375-9239394 MsG0580024754.01.T01:CDS 35.0%
GTATTGTCCAATAAGAAGAG+AGG - Chr5:9238981-9239000 MsG0580024754.01.T01:CDS 35.0%
GTCAAAACAACTCAACTCAA+AGG + Chr5:9239356-9239375 None:intergenic 35.0%
GTGGATCTTGCTCAAAATAT+TGG + Chr5:9239226-9239245 None:intergenic 35.0%
TACCAATAGAGAGAGAAAGA+GGG + Chr5:9238964-9238983 None:intergenic 35.0%
TATTCTGAATGTGACACTGA+TGG + Chr5:9240352-9240371 None:intergenic 35.0%
! CCTTTTTCCTCTCTTCTTAT+TGG + Chr5:9238991-9239010 None:intergenic 35.0%
! GAATCTCAATGGTGTTGAAA+AGG - Chr5:9238738-9238757 MsG0580024754.01.T01:three_prime_UTR 35.0%
! GTTAAATTTCGTATGGGTTG+GGG - Chr5:9241148-9241167 MsG0580024754.01.T01:intron 35.0%
! GTTGGCATTCAATTCTTTTC+TGG - Chr5:9240476-9240495 MsG0580024754.01.T01:intron 35.0%
! GTTGTGGTATGTGTTGTTAA+AGG - Chr5:9240886-9240905 MsG0580024754.01.T01:intron 35.0%
! TGAATTGCGTCTGAAATCTT+GGG - Chr5:9240850-9240869 MsG0580024754.01.T01:intron 35.0%
! TTCAGTAACATTGGTTGTGT+TGG - Chr5:9240458-9240477 MsG0580024754.01.T01:intron 35.0%
!! TTAAATTTCGTATGGGTTGG+GGG - Chr5:9241149-9241168 MsG0580024754.01.T01:intron 35.0%
!! TTTTGGGTATGCTGTAAGAT+AGG - Chr5:9241363-9241382 MsG0580024754.01.T01:intron 35.0%
!!! AGTGGAATACTGTTTTGATC+TGG + Chr5:9240594-9240613 None:intergenic 35.0%
!!! GTCATTTTAATTTAGCGAGC+TGG - Chr5:9240137-9240156 MsG0580024754.01.T01:intron 35.0%
AAACAGTATTCCACTACAGC+TGG - Chr5:9240599-9240618 MsG0580024754.01.T01:intron 40.0%
ACAATGGTTAAGTGACTCTC+AGG + Chr5:9241497-9241516 None:intergenic 40.0%
ACTCAAAGGTTGTCAAGAAC+TGG + Chr5:9239342-9239361 None:intergenic 40.0%
ATGGTGAGTGGGATGAAAAT+TGG + Chr5:9240333-9240352 None:intergenic 40.0%
CAAAAGTGGTGACTCAATGA+TGG + Chr5:9239779-9239798 None:intergenic 40.0%
CAACTCCGACCTAACAAAAA+TGG + Chr5:9240821-9240840 None:intergenic 40.0%
CAATGGTTAAGTGACTCTCA+GGG + Chr5:9241496-9241515 None:intergenic 40.0%
CATTGATATAGGAATCGCAG+TGG - Chr5:9241056-9241075 MsG0580024754.01.T01:intron 40.0%
CTCAAAGGTTGTCAAGAACT+GGG + Chr5:9239341-9239360 None:intergenic 40.0%
GACCTGTGATTGTTATCAAC+CGG - Chr5:9239658-9239677 MsG0580024754.01.T01:CDS 40.0%
GATGTTGGCAAGAATCTCAA+TGG - Chr5:9238727-9238746 MsG0580024754.01.T01:three_prime_UTR 40.0%
GCTCAAAATATTGGAAGGAG+TGG + Chr5:9239217-9239236 None:intergenic 40.0%
TACATTCATGGTTGGCAGTT+GGG + Chr5:9239805-9239824 None:intergenic 40.0%
TGAAAGAAAGGATGTTGCTG+TGG - Chr5:9239436-9239455 MsG0580024754.01.T01:intron 40.0%
TTACATTCATGGTTGGCAGT+TGG + Chr5:9239806-9239825 None:intergenic 40.0%
TTCCCTCTTTCTCTCTCTAT+TGG - Chr5:9238959-9238978 MsG0580024754.01.T01:CDS 40.0%
TTTCCTGCAAAAGAAGCAAG+TGG + Chr5:9239956-9239975 None:intergenic 40.0%
! AAGTACAACGAGAGTATGAG+AGG + Chr5:9238928-9238947 None:intergenic 40.0%
! ACTTGCTTCTTTTGCAGGAA+AGG - Chr5:9239955-9239974 MsG0580024754.01.T01:intron 40.0%
! ATACCACTTGCTTCTTTTGC+AGG - Chr5:9239950-9239969 MsG0580024754.01.T01:intron 40.0%
! CATCATTGAGTCACCACTTT+TGG - Chr5:9239777-9239796 MsG0580024754.01.T01:intron 40.0%
! CATTTTTGTTAGGTCGGAGT+TGG - Chr5:9240819-9240838 MsG0580024754.01.T01:intron 40.0%
! CTGAATTGCGTCTGAAATCT+TGG - Chr5:9240849-9240868 MsG0580024754.01.T01:intron 40.0%
! CTTTTACACCCACACATGTA+GGG + Chr5:9240254-9240273 None:intergenic 40.0%
! GCCTCTGTCATTAGAGTTTT+TGG - Chr5:9240287-9240306 MsG0580024754.01.T01:intron 40.0%
! GTGGTGCTTTTATCAACGTT+TGG - Chr5:9241078-9241097 MsG0580024754.01.T01:intron 40.0%
! TCTTTTACACCCACACATGT+AGG + Chr5:9240255-9240274 None:intergenic 40.0%
! TGTATTTGAACAGACTGGCT+TGG + Chr5:9241423-9241442 None:intergenic 40.0%
!! TAAATTTCGTATGGGTTGGG+GGG - Chr5:9241150-9241169 MsG0580024754.01.T01:intron 40.0%
!! TGAAAAGGTGTCAAGTTGTG+AGG - Chr5:9238753-9238772 MsG0580024754.01.T01:three_prime_UTR 40.0%
!! TTCCGGTTGATAACAATCAC+AGG + Chr5:9239663-9239682 None:intergenic 40.0%
AACGAGACACCAGTTGTCTA+TGG - Chr5:9240668-9240687 MsG0580024754.01.T01:intron 45.0%
AACTCGATCCCTACATGTGT+GGG - Chr5:9240243-9240262 MsG0580024754.01.T01:intron 45.0%
AATACCTTAGGTAAGCGCTC+CGG + Chr5:9240974-9240993 None:intergenic 45.0%
ACACATGTAGGGATCGAGTT+GGG + Chr5:9240243-9240262 None:intergenic 45.0%
ACATTCATGGTTGGCAGTTG+GGG + Chr5:9239804-9239823 None:intergenic 45.0%
ATGTACATCAGTTGCTCCTC+TGG + Chr5:9239557-9239576 None:intergenic 45.0%
CAAGATCCACCATTGTTCTC+TGG - Chr5:9239236-9239255 MsG0580024754.01.T01:intron 45.0%
CAGTAAGCACATGCTTGATG+CGG + Chr5:9241246-9241265 None:intergenic 45.0%
CTCGTGAACATTCTGCAGAT+GGG + Chr5:9241532-9241551 None:intergenic 45.0%
GGGATGACCTTCATAAGTTG+TGG - Chr5:9240870-9240889 MsG0580024754.01.T01:intron 45.0%
TAGAAGCCAGAGAACAATGG+TGG + Chr5:9239245-9239264 None:intergenic 45.0%
TCGTGAACATTCTGCAGATG+GGG + Chr5:9241531-9241550 None:intergenic 45.0%
TGAAAGCCAGAAAGCTCACA+GGG + Chr5:9240163-9240182 None:intergenic 45.0%
TGATATAGGAATCGCAGTGG+TGG - Chr5:9241059-9241078 MsG0580024754.01.T01:intron 45.0%
TGCTGTTAGAAACCCTCTTC+CGG - Chr5:9240622-9240641 MsG0580024754.01.T01:intron 45.0%
TGGGGTTCATTTGACGACAA+TGG + Chr5:9241513-9241532 None:intergenic 45.0%
TGTACATCAGTTGCTCCTCT+GGG + Chr5:9239556-9239575 None:intergenic 45.0%
TTTGTTAGGTCGGAGTTGGA+AGG - Chr5:9240823-9240842 MsG0580024754.01.T01:intron 45.0%
! AATGTGACACTGATGGTGAG+TGG + Chr5:9240345-9240364 None:intergenic 45.0%
! ATGTGACACTGATGGTGAGT+GGG + Chr5:9240344-9240363 None:intergenic 45.0%
! TGGCATTGCAGCCAATGTTT+TGG - Chr5:9240532-9240551 MsG0580024754.01.T01:intron 45.0%
ACTCATGTGCTAGAGCTGAG+TGG - Chr5:9240182-9240201 MsG0580024754.01.T01:intron 50.0%
ATACCTTAGGTAAGCGCTCC+GGG + Chr5:9240973-9240992 None:intergenic 50.0%
CAACACGCGTAAATCAGACC+AGG + Chr5:9241011-9241030 None:intergenic 50.0%
CAACTCGATCCCTACATGTG+TGG - Chr5:9240242-9240261 MsG0580024754.01.T01:intron 50.0%
CACACATGTAGGGATCGAGT+TGG + Chr5:9240244-9240263 None:intergenic 50.0%
CATTCATGGTTGGCAGTTGG+GGG + Chr5:9239803-9239822 None:intergenic 50.0%
CCTCAAAGAACTCCGGAAGA+GGG + Chr5:9240637-9240656 None:intergenic 50.0%
CGATTGAAGAACCAGACGCA+AGG - Chr5:9241272-9241291 MsG0580024754.01.T01:intron 50.0%
CTCATGTGCTAGAGCTGAGT+GGG - Chr5:9240183-9240202 MsG0580024754.01.T01:intron 50.0%
CTGTCTGCCTCAAAGAACTC+CGG + Chr5:9240644-9240663 None:intergenic 50.0%
GAACTTGCGCTTTCCTAACC+CGG - Chr5:9240952-9240971 MsG0580024754.01.T01:intron 50.0%
GCTCGTGAACATTCTGCAGA+TGG + Chr5:9241533-9241552 None:intergenic 50.0%
GTGAAAGCCAGAAAGCTCAC+AGG + Chr5:9240164-9240183 None:intergenic 50.0%
TCTAACAGCACCAGCTGTAG+TGG + Chr5:9240612-9240631 None:intergenic 50.0%
TTGAAGAACCAGACGCAAGG+AGG - Chr5:9241275-9241294 MsG0580024754.01.T01:intron 50.0%
CCCTCTTCCGGAGTTCTTTG+AGG - Chr5:9240634-9240653 MsG0580024754.01.T01:intron 55.0%
GCCTCAAAGAACTCCGGAAG+AGG + Chr5:9240638-9240657 None:intergenic 55.0%
GGTTGGGGGGAAACTGTTCA+CGG - Chr5:9241163-9241182 MsG0580024754.01.T01:intron 55.0%
TGTGCTAGAGCTGAGTGGGA+TGG - Chr5:9240187-9240206 MsG0580024754.01.T01:intron 55.0%
TTAGGTAAGCGCTCCGGGTT+AGG + Chr5:9240968-9240987 None:intergenic 55.0%
! TAACCCGGAGCGCTTACCTA+AGG - Chr5:9240967-9240986 MsG0580024754.01.T01:intron 55.0%
AGCTGGCCCTGTGAGCTTTC+TGG - Chr5:9240154-9240173 MsG0580024754.01.T01:intron 60.0%
TCAGCAGACCTCCTTGCGTC+TGG + Chr5:9241286-9241305 None:intergenic 60.0%
TGGCAGTTGGGGGCCAAAAG+TGG + Chr5:9239793-9239812 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 9238676 9241599 9238676 ID=MsG0580024754.01;Name=MsG0580024754.01
Chr5 mRNA 9238676 9241599 9238676 ID=MsG0580024754.01.T01;Parent=MsG0580024754.01;Name=MsG0580024754.01.T01;_AED=0.38;_eAED=0.49;_QI=0|0.25|0.4|1|1|1|5|266|213
Chr5 exon 9238676 9239049 9238676 ID=MsG0580024754.01.T01:exon:19547;Parent=MsG0580024754.01.T01
Chr5 exon 9239287 9239444 9239287 ID=MsG0580024754.01.T01:exon:19546;Parent=MsG0580024754.01.T01
Chr5 exon 9239508 9239732 9239508 ID=MsG0580024754.01.T01:exon:19545;Parent=MsG0580024754.01.T01
Chr5 exon 9240067 9240123 9240067 ID=MsG0580024754.01.T01:exon:19544;Parent=MsG0580024754.01.T01
Chr5 exon 9241506 9241599 9241506 ID=MsG0580024754.01.T01:exon:19543;Parent=MsG0580024754.01.T01
Chr5 CDS 9241506 9241599 9241506 ID=MsG0580024754.01.T01:cds;Parent=MsG0580024754.01.T01
Chr5 CDS 9240067 9240123 9240067 ID=MsG0580024754.01.T01:cds;Parent=MsG0580024754.01.T01
Chr5 CDS 9239508 9239732 9239508 ID=MsG0580024754.01.T01:cds;Parent=MsG0580024754.01.T01
Chr5 CDS 9239287 9239444 9239287 ID=MsG0580024754.01.T01:cds;Parent=MsG0580024754.01.T01
Chr5 CDS 9238942 9239049 9238942 ID=MsG0580024754.01.T01:cds;Parent=MsG0580024754.01.T01
Chr5 three_prime_UTR 9238676 9238941 9238676 ID=MsG0580024754.01.T01:three_prime_utr;Parent=MsG0580024754.01.T01
Gene Sequence

>MsG0580024754.01.T01

ATGTCTCTTTTTATCAAGTCGATTAGCAATTTAGTAAGTCTTTGTATTGAAGATGTTGGCAAGAATCTCAATGGTGTTGAAAAGGTGTCAAGTTCGAGCTGGCCCTGTGAGCTTTCTGGCTTTCACTCATGTGCTAGAGCTGAGTGGGATGCCAATGTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTATGAAATATGCACCTCTTCTTTTCAATAGAATAGGAATTAAAAATCTCACTGATTTTCATTATAGCCACACATTAGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCAAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA
Protein sequence

>MsG0580024754.01.T01

MSLFIKSISNLVSLCIEDVGKNLNGVEKVSSSSWPCELSGFHSCARAEWDANVLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGMKYAPLLFNRIGIKNLTDFHYSHTLGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL*