AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0008050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0008050 MtrunA17_Chr1g0146921 91.943 211 16 1 1 211 1 210 1.74e-140 390
Msa0008050 MtrunA17_Chr3g0126981 58.857 175 57 5 37 210 1 161 5.61e-58 180
Msa0008050 MtrunA17_Chr8g0346691 35.519 183 61 11 43 192 35 193 1.21e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0008050 AT5G53030.1 40.099 202 76 10 45 207 40 235 1.10e-27 105
Msa0008050 AT4G27810.1 35.514 214 95 8 9 207 1 186 1.37e-21 88.2
Msa0008050 AT5G53030.2 38.503 187 74 9 45 196 40 220 1.66e-21 89.0

Find 70 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTATAAAACCGTAAATT+TGG 0.134308 1_1:+11616183 None:intergenic
AACAAGGGCAGTGTGAATTT+TGG 0.173203 1_1:-11618002 Msa0008050:CDS
TAACTGCAGGCAAGTATTTA+TGG 0.195534 1_1:-11616379 Msa0008050:intron
TCCTCCTAGGTTGTTGTCTT+TGG 0.204117 1_1:-11618132 Msa0008050:CDS
ATAGAAGTGGTAATGTTATT+TGG 0.209105 1_1:+11616245 None:intergenic
GGATTTCAAATGTTAGATTA+TGG 0.250519 1_1:-11616039 Msa0008050:three_prime_UTR
CAAAACTAGGTCCAAATTTA+CGG 0.283967 1_1:-11616194 Msa0008050:three_prime_UTR
TTGTTAACTTCTCTATCTTT+TGG 0.318510 1_1:+11618176 None:intergenic
TTTAGCTTATATATAATATA+GGG 0.319993 1_1:+11616150 None:intergenic
TAATGTTATTTGGAGGAAAA+AGG 0.320524 1_1:+11616255 None:intergenic
CGATAATACTAGTATATCTT+AGG 0.338623 1_1:-11616065 Msa0008050:three_prime_UTR
ACCATCACCAACAACTGTTT+TGG 0.363972 1_1:-11618081 Msa0008050:CDS
CACTATAGTTCCATCTGATT+CGG 0.380777 1_1:-11618445 Msa0008050:five_prime_UTR
TAACAACGTTGTTAGAGCTT+TGG 0.381783 1_1:-11618159 Msa0008050:CDS
CACATGGTACTTCACATTTG+TGG 0.395630 1_1:-11617848 Msa0008050:CDS
TTGGGTCTAGTAGGTGGGGT+GGG 0.401963 1_1:-11617983 Msa0008050:CDS
ATCATGATGATGATGAATTA+AGG 0.404054 1_1:-11618364 Msa0008050:CDS
ACATGGTACTTCACATTTGT+GGG 0.406088 1_1:-11617847 Msa0008050:intron
ATAGCCGTCGTTCGCGGTGG+TGG 0.414893 1_1:+11618263 None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG 0.452572 1_1:-11617917 Msa0008050:CDS
AAAATAATAATATTAGCATA+TGG 0.462348 1_1:-11615999 Msa0008050:three_prime_UTR
TTTAAGTGGGAGGAAGCACC+AGG 0.462547 1_1:-11618230 Msa0008050:CDS
TTTCTTGTTTGCGCCTCCAT+GGG 0.468492 1_1:+11616338 None:intergenic
CACAGCCGCGTTAACGTTGG+TGG 0.470944 1_1:+11618308 None:intergenic
GTCGTTCGCGGTGGTGGAGT+TGG 0.473237 1_1:+11618269 None:intergenic
TGTTTATTTATAGAGGGTTG+TGG 0.473706 1_1:+11618486 None:intergenic
TAGGTTGTTGTCTTTGGAGA+GGG 0.474181 1_1:-11618126 Msa0008050:CDS
GGACCTTCCAAAACAGTTGT+TGG 0.481823 1_1:+11618074 None:intergenic
TGACATGACCTAGGCTTCCC+TGG 0.485647 1_1:+11618212 None:intergenic
GGTGCTTCCTCCCACTTAAA+CGG 0.493829 1_1:+11618233 None:intergenic
TGTGTGGTGTTTATTTATAG+AGG 0.493975 1_1:+11618479 None:intergenic
GAGCTTTGGAACTTCCTCCT+AGG 0.498331 1_1:-11618145 Msa0008050:CDS
TTTGGGTCTAGTAGGTGGGG+TGG 0.500229 1_1:-11617984 Msa0008050:CDS
GACACTGCACGTCCAACGTA+TGG 0.515972 1_1:+11618053 None:intergenic
CTCTTCCACCAACGTTAACG+CGG 0.522519 1_1:-11618313 Msa0008050:CDS
GATCACAAGAGTTAAGAGAA+GGG 0.526664 1_1:-11617880 Msa0008050:CDS
GAAGTGGTAATGTTATTTGG+AGG 0.528518 1_1:+11616248 None:intergenic
CTAGGTTGTTGTCTTTGGAG+AGG 0.533267 1_1:-11618127 Msa0008050:CDS
TTCAAGTTGGAGTGTTGATG+GGG 0.539072 1_1:-11617916 Msa0008050:CDS
CTATATCGATACCGTTTAAG+TGG 0.539738 1_1:-11618244 Msa0008050:CDS
AACTCCACCACCGCGAACGA+CGG 0.539846 1_1:-11618267 Msa0008050:CDS
AGGAAGCACCAGGGAAGCCT+AGG 0.540552 1_1:-11618220 Msa0008050:CDS
ATACTAGTATATCTTAGGAA+TGG 0.546759 1_1:-11616060 Msa0008050:three_prime_UTR
GTGTGGTGTTTATTTATAGA+GGG 0.552822 1_1:+11618480 None:intergenic
GCTTTAAGCAAGTGGTCCCA+TGG 0.553111 1_1:-11616354 Msa0008050:CDS
AGACGGTTCTAACTTTAGAT+CGG 0.553929 1_1:+11616284 None:intergenic
ATCGATATAGCCGTCGTTCG+CGG 0.559277 1_1:+11618257 None:intergenic
TTATGGAAGCTTTAAGCAAG+TGG 0.560654 1_1:-11616362 Msa0008050:CDS
AGATCACAAGAGTTAAGAGA+AGG 0.565850 1_1:-11617881 Msa0008050:CDS
ACGACAAAGACCGAATCAGA+TGG 0.583224 1_1:+11618435 None:intergenic
ATCACAAGAGTTAAGAGAAG+GGG 0.589130 1_1:-11617879 Msa0008050:CDS
TCCAAAACAGTTGTTGGTGA+TGG 0.590521 1_1:+11618080 None:intergenic
TGCTACTATACAAATAGAAG+TGG 0.594811 1_1:+11616232 None:intergenic
ATCGATACCGTTTAAGTGGG+AGG 0.600972 1_1:-11618240 Msa0008050:CDS
AATATGAAAACATCAAAACT+AGG 0.601832 1_1:-11616207 Msa0008050:three_prime_UTR
GTATAGGGATAGTAATAACA+AGG 0.626999 1_1:-11618018 Msa0008050:CDS
TCCAAAGACAACAACCTAGG+AGG 0.639929 1_1:+11618131 None:intergenic
GGTCGTGTATGACATGACCT+AGG 0.639981 1_1:+11618203 None:intergenic
TATATCGATACCGTTTAAGT+GGG 0.641926 1_1:-11618243 Msa0008050:CDS
TCAAGTTGGAGTGTTGATGG+GGG 0.642096 1_1:-11617915 Msa0008050:CDS
AAAAGTCAAAACTACCCTCA+CGG 0.642581 1_1:+11617937 None:intergenic
AATAATAGAATTGACCGTGA+GGG 0.656653 1_1:-11617951 Msa0008050:CDS
AAACACAGCCGCGTTAACGT+TGG 0.660970 1_1:+11618305 None:intergenic
CTCTCCAAAGACAACAACCT+AGG 0.661580 1_1:+11618128 None:intergenic
AAATAATAGAATTGACCGTG+AGG 0.684752 1_1:-11617952 Msa0008050:CDS
GATATAGCCGTCGTTCGCGG+TGG 0.697862 1_1:+11618260 None:intergenic
TATAGGGATAGTAATAACAA+GGG 0.698970 1_1:-11618017 Msa0008050:CDS
AGAAGGGGAAGCTTCTCACA+TGG 0.717228 1_1:-11617864 Msa0008050:CDS
TTAAGCAAGTGGTCCCATGG+AGG 0.738180 1_1:-11616351 Msa0008050:CDS
TTAAGTGGGAGGAAGCACCA+GGG 0.769568 1_1:-11618229 Msa0008050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTAAAAAAGTTTTTTAT+AGG - chr1_1:11616719-11616738 Msa0008050:intron 10.0%
!! ATAATTTATACAACAATTAA+TGG - chr1_1:11616756-11616775 Msa0008050:intron 10.0%
!!! TTTAGCTTATATATAATATA+GGG + chr1_1:11618308-11618327 None:intergenic 10.0%
!!! TTTTAGCTTATATATAATAT+AGG + chr1_1:11618309-11618328 None:intergenic 10.0%
!! AAAATAATAATATTAGCATA+TGG - chr1_1:11618456-11618475 Msa0008050:five_prime_UTR 10.0%
!! TAAGGTATATAAAATAAGAT+AGG + chr1_1:11616646-11616665 None:intergenic 15.0%
!!! CTAATTTTTATGCAATTTAT+TGG - chr1_1:11616852-11616871 Msa0008050:intron 15.0%
!! AAAACTATATAATTTAGCTA+AGG + chr1_1:11616888-11616907 None:intergenic 15.0%
!!! TAGCTAAATTATATAGTTTT+TGG - chr1_1:11616888-11616907 Msa0008050:intron 15.0%
!!! ATTGTTGGTTAAAATATTAA+TGG - chr1_1:11617040-11617059 Msa0008050:intron 15.0%
!! ATTAAAACATCAATATCATT+TGG + chr1_1:11617619-11617638 None:intergenic 15.0%
!! TGAACATTATCATTTATAAT+TGG - chr1_1:11618008-11618027 Msa0008050:CDS 15.0%
!! ATTATCATTTATAATTGGTT+TGG - chr1_1:11618013-11618032 Msa0008050:CDS 15.0%
!!! ATATAGTTTTTGGATGATTT+TGG - chr1_1:11616898-11616917 Msa0008050:intron 20.0%
!! TAAAACCAAAAACCATAAAT+GGG + chr1_1:11617143-11617162 None:intergenic 20.0%
!! ATAAAACCAAAAACCATAAA+TGG + chr1_1:11617144-11617163 None:intergenic 20.0%
!!! ATTTATGGTTTTTGGTTTTA+TGG - chr1_1:11617143-11617162 Msa0008050:intron 20.0%
!!! AGAAAAAGGAAAAACTTTTA+GGG + chr1_1:11617505-11617524 None:intergenic 20.0%
!!! TAGAAAAAGGAAAAACTTTT+AGG + chr1_1:11617506-11617525 None:intergenic 20.0%
!! TGTGTGTATTTAATAGAAAA+AGG + chr1_1:11617519-11617538 None:intergenic 20.0%
!!! ACATGTATCTAAAATATGAT+TGG - chr1_1:11617536-11617555 Msa0008050:intron 20.0%
!! CTATTAATATCATTGCATTA+GGG - chr1_1:11617691-11617710 Msa0008050:intron 20.0%
!!! TTTTTGTCTATTTAAGTTCA+TGG - chr1_1:11617722-11617741 Msa0008050:intron 20.0%
!!! TTGAGTTTTACTACTTTTAT+TGG + chr1_1:11617758-11617777 None:intergenic 20.0%
!! TTATTTATAAATGTGATCAG+AGG - chr1_1:11617832-11617851 Msa0008050:intron 20.0%
!! TATTTATAAATGTGATCAGA+GGG - chr1_1:11617833-11617852 Msa0008050:intron 20.0%
!! AATATGAAAACATCAAAACT+AGG - chr1_1:11618248-11618267 Msa0008050:CDS 20.0%
!! AACTTATAAAACCGTAAATT+TGG + chr1_1:11618275-11618294 None:intergenic 20.0%
!!! TAGTGAAATATTTTGATGTG+TGG + chr1_1:11615995-11616014 None:intergenic 25.0%
!! ATCATGATGATGATGAATTA+AGG - chr1_1:11616091-11616110 Msa0008050:three_prime_UTR 25.0%
!! TTGTTAACTTCTCTATCTTT+TGG + chr1_1:11616282-11616301 None:intergenic 25.0%
! TATAGGGATAGTAATAACAA+GGG - chr1_1:11616438-11616457 Msa0008050:intron 25.0%
! ATTGTAGTTTCAAATGCTTA+GGG - chr1_1:11616691-11616710 Msa0008050:intron 25.0%
! ATATTAGAAAATTTGACCGA+TGG - chr1_1:11616788-11616807 Msa0008050:intron 25.0%
!! TAATCTAACTTTTACTCCAT+CGG + chr1_1:11616807-11616826 None:intergenic 25.0%
! AATTAATTTCATGACTCTAG+GGG + chr1_1:11616958-11616977 None:intergenic 25.0%
! GAATTAATTTCATGACTCTA+GGG + chr1_1:11616959-11616978 None:intergenic 25.0%
! AGAATTAATTTCATGACTCT+AGG + chr1_1:11616960-11616979 None:intergenic 25.0%
!! TGATTAAGTGCAATCATTTT+TGG - chr1_1:11616993-11617012 Msa0008050:intron 25.0%
! TGTATAATCCTTAGGAAATA+AGG - chr1_1:11617213-11617232 Msa0008050:intron 25.0%
!! ATAAGGTATTTCGTAGATAA+AGG - chr1_1:11617230-11617249 Msa0008050:intron 25.0%
! GCTATTAATATCATTGCATT+AGG - chr1_1:11617690-11617709 Msa0008050:intron 25.0%
!!! ACTACTTTTATTGGAAGAAT+TGG + chr1_1:11617749-11617768 None:intergenic 25.0%
! ATTATTAACTTCATGACACT+AGG - chr1_1:11617779-11617798 Msa0008050:intron 25.0%
! TTATTAACTTCATGACACTA+GGG - chr1_1:11617780-11617799 Msa0008050:intron 25.0%
!! TTTTGTATTGCTATGACTTT+TGG - chr1_1:11617972-11617991 Msa0008050:CDS 25.0%
!! TAATGTTATTTGGAGGAAAA+AGG + chr1_1:11618203-11618222 None:intergenic 25.0%
!! ATAGAAGTGGTAATGTTATT+TGG + chr1_1:11618213-11618232 None:intergenic 25.0%
! CGATAATACTAGTATATCTT+AGG - chr1_1:11618390-11618409 Msa0008050:CDS 25.0%
! ATACTAGTATATCTTAGGAA+TGG - chr1_1:11618395-11618414 Msa0008050:CDS 25.0%
! GGATTTCAAATGTTAGATTA+TGG - chr1_1:11618416-11618435 Msa0008050:five_prime_UTR 25.0%
GTGTGGTGTTTATTTATAGA+GGG + chr1_1:11615978-11615997 None:intergenic 30.0%
TGTGTGGTGTTTATTTATAG+AGG + chr1_1:11615979-11615998 None:intergenic 30.0%
TATATCGATACCGTTTAAGT+GGG - chr1_1:11616212-11616231 Msa0008050:three_prime_UTR 30.0%
GTATAGGGATAGTAATAACA+AGG - chr1_1:11616437-11616456 Msa0008050:intron 30.0%
AAATAATAGAATTGACCGTG+AGG - chr1_1:11616503-11616522 Msa0008050:intron 30.0%
AATAATAGAATTGACCGTGA+GGG - chr1_1:11616504-11616523 Msa0008050:intron 30.0%
!!! GTAGTTTTGACTTTTCAAGT+TGG - chr1_1:11616526-11616545 Msa0008050:intron 30.0%
GATTGTAGTTTCAAATGCTT+AGG - chr1_1:11616690-11616709 Msa0008050:intron 30.0%
!!! TAAGTGCAATCATTTTTGGT+CGG - chr1_1:11616997-11617016 Msa0008050:intron 30.0%
!!! ATCATTTTTGGTCGGTTTTT+TGG - chr1_1:11617005-11617024 Msa0008050:intron 30.0%
! TGGATGATTTGTGAAATTGT+TGG - chr1_1:11617025-11617044 Msa0008050:intron 30.0%
TTGATATACTGTTGTTGTGA+AGG - chr1_1:11617078-11617097 Msa0008050:intron 30.0%
! TTTTATGGTTGCTTACTAGT+TGG - chr1_1:11617158-11617177 Msa0008050:intron 30.0%
GTGAATCATGTATAATCCTT+AGG - chr1_1:11617205-11617224 Msa0008050:intron 30.0%
! ACGAAATACCTTATTTCCTA+AGG + chr1_1:11617224-11617243 None:intergenic 30.0%
AACGTTCAACTGGAAATAAA+TGG - chr1_1:11617254-11617273 Msa0008050:intron 30.0%
! CATGTTAGTGGATAATACTT+GGG + chr1_1:11617463-11617482 None:intergenic 30.0%
! ACATGTTAGTGGATAATACT+TGG + chr1_1:11617464-11617483 None:intergenic 30.0%
AAGTATTATCCACTAACATG+TGG - chr1_1:11617463-11617482 Msa0008050:intron 30.0%
AATCTTTGACCATACTATCA+TGG - chr1_1:11617651-11617670 Msa0008050:intron 30.0%
ATATTAATAGCCACTCCATT+AGG + chr1_1:11617681-11617700 None:intergenic 30.0%
TATTAACTTCATGACACTAG+GGG - chr1_1:11617781-11617800 Msa0008050:intron 30.0%
!!! CATTCATCGCAGTTATTTTT+CGG + chr1_1:11617865-11617884 None:intergenic 30.0%
! AGAAACACTTTTATAACTGC+AGG - chr1_1:11618063-11618082 Msa0008050:CDS 30.0%
TGCTACTATACAAATAGAAG+TGG + chr1_1:11618226-11618245 None:intergenic 30.0%
CAAAACTAGGTCCAAATTTA+CGG - chr1_1:11618261-11618280 Msa0008050:CDS 30.0%
CACTATAGTTCCATCTGATT+CGG - chr1_1:11616010-11616029 Msa0008050:three_prime_UTR 35.0%
CTATATCGATACCGTTTAAG+TGG - chr1_1:11616211-11616230 Msa0008050:three_prime_UTR 35.0%
TAACAACGTTGTTAGAGCTT+TGG - chr1_1:11616296-11616315 Msa0008050:three_prime_UTR 35.0%
AAAAGTCAAAACTACCCTCA+CGG + chr1_1:11616521-11616540 None:intergenic 35.0%
!!! TTTTCAAGTTGGAGTGTTGA+TGG - chr1_1:11616537-11616556 Msa0008050:intron 35.0%
!! TTTCAAGTTGGAGTGTTGAT+GGG - chr1_1:11616538-11616557 Msa0008050:intron 35.0%
AGATCACAAGAGTTAAGAGA+AGG - chr1_1:11616574-11616593 Msa0008050:intron 35.0%
GATCACAAGAGTTAAGAGAA+GGG - chr1_1:11616575-11616594 Msa0008050:intron 35.0%
ATCACAAGAGTTAAGAGAAG+GGG - chr1_1:11616576-11616595 Msa0008050:intron 35.0%
ACATGGTACTTCACATTTGT+GGG - chr1_1:11616608-11616627 Msa0008050:intron 35.0%
! TCCATCCCATTTATGGTTTT+TGG - chr1_1:11617135-11617154 Msa0008050:intron 35.0%
ACCAAAAACCATAAATGGGA+TGG + chr1_1:11617139-11617158 None:intergenic 35.0%
AGATAAAGGTAACGTTCAAC+TGG - chr1_1:11617244-11617263 Msa0008050:intron 35.0%
TTGTGAAACTTCACCAACAT+GGG - chr1_1:11617283-11617302 Msa0008050:intron 35.0%
TAAAGACACTTGTGACATTG+TGG + chr1_1:11617349-11617368 None:intergenic 35.0%
ATAATACTTGGGTGACATTG+TGG + chr1_1:11617452-11617471 None:intergenic 35.0%
TAACTGCAGGCAAGTATTTA+TGG - chr1_1:11618076-11618095 Msa0008050:CDS 35.0%
!! TTATGGAAGCTTTAAGCAAG+TGG - chr1_1:11618093-11618112 Msa0008050:CDS 35.0%
! AGACGGTTCTAACTTTAGAT+CGG + chr1_1:11618174-11618193 None:intergenic 35.0%
GAGGAAAAAGGTAAGAAAGA+CGG + chr1_1:11618191-11618210 None:intergenic 35.0%
! GAAGTGGTAATGTTATTTGG+AGG + chr1_1:11618210-11618229 None:intergenic 35.0%
! GGTGGAAGAGAAAGTTTTTG+TGG + chr1_1:11616132-11616151 None:intergenic 40.0%
!!! ACTTCTCTATCTTTTGGCTC+TGG + chr1_1:11616276-11616295 None:intergenic 40.0%
! TAGGTTGTTGTCTTTGGAGA+GGG - chr1_1:11616329-11616348 Msa0008050:CDS 40.0%
! ACCATCACCAACAACTGTTT+TGG - chr1_1:11616374-11616393 Msa0008050:intron 40.0%
! TCCAAAACAGTTGTTGGTGA+TGG + chr1_1:11616378-11616397 None:intergenic 40.0%
!! TCACCAACAACTGTTTTGGA+AGG - chr1_1:11616378-11616397 Msa0008050:intron 40.0%
! TGTCTTTTACGACGTCGTAT+AGG - chr1_1:11616421-11616440 Msa0008050:intron 40.0%
! GTCTTTTACGACGTCGTATA+GGG - chr1_1:11616422-11616441 Msa0008050:intron 40.0%
! AACAAGGGCAGTGTGAATTT+TGG - chr1_1:11616453-11616472 Msa0008050:intron 40.0%
! ACAAGGGCAGTGTGAATTTT+GGG - chr1_1:11616454-11616473 Msa0008050:intron 40.0%
!! GTGTGAATTTTGGGTCTAGT+AGG - chr1_1:11616463-11616482 Msa0008050:intron 40.0%
!! TGAATTTTGGGTCTAGTAGG+TGG - chr1_1:11616466-11616485 Msa0008050:intron 40.0%
!! GAATTTTGGGTCTAGTAGGT+GGG - chr1_1:11616467-11616486 Msa0008050:intron 40.0%
!! AATTTTGGGTCTAGTAGGTG+GGG - chr1_1:11616468-11616487 Msa0008050:intron 40.0%
!! TTCAAGTTGGAGTGTTGATG+GGG - chr1_1:11616539-11616558 Msa0008050:intron 40.0%
CACATGGTACTTCACATTTG+TGG - chr1_1:11616607-11616626 Msa0008050:intron 40.0%
GAGTGAGACATCAATGGATA+AGG + chr1_1:11616664-11616683 None:intergenic 40.0%
CATGAAGAGTGAGACATCAA+TGG + chr1_1:11616670-11616689 None:intergenic 40.0%
CATTGATGTCTCACTCTTCA+TGG - chr1_1:11616668-11616687 Msa0008050:intron 40.0%
AACTTTGATACAGTGTGCTG+TGG - chr1_1:11617112-11617131 Msa0008050:intron 40.0%
CTTGTGAAACTTCACCAACA+TGG - chr1_1:11617282-11617301 Msa0008050:intron 40.0%
TGTGAAACTTCACCAACATG+GGG - chr1_1:11617284-11617303 Msa0008050:intron 40.0%
TTTGTACACGTGTGACATTG+TGG - chr1_1:11617400-11617419 Msa0008050:intron 40.0%
!!! TTTTAGGGTCATGCTGAAGA+TGG + chr1_1:11617490-11617509 None:intergenic 40.0%
TTTGACCATACTATCATGGC+CGG - chr1_1:11617655-11617674 Msa0008050:intron 40.0%
ACGACAAAGACCGAATCAGA+TGG + chr1_1:11616023-11616042 None:intergenic 45.0%
ATCGATACCGTTTAAGTGGG+AGG - chr1_1:11616215-11616234 Msa0008050:three_prime_UTR 45.0%
TCCTCCTAGGTTGTTGTCTT+TGG - chr1_1:11616323-11616342 Msa0008050:exon 45.0%
TCCAAAGACAACAACCTAGG+AGG + chr1_1:11616327-11616346 None:intergenic 45.0%
CTCTCCAAAGACAACAACCT+AGG + chr1_1:11616330-11616349 None:intergenic 45.0%
! CTAGGTTGTTGTCTTTGGAG+AGG - chr1_1:11616328-11616347 Msa0008050:CDS 45.0%
GGACCTTCCAAAACAGTTGT+TGG + chr1_1:11616384-11616403 None:intergenic 45.0%
!!! GTTTTGGAAGGTCCATACGT+TGG - chr1_1:11616390-11616409 Msa0008050:intron 45.0%
!! TCAAGTTGGAGTGTTGATGG+GGG - chr1_1:11616540-11616559 Msa0008050:intron 45.0%
!! GACATTGTGGTTGGTGACAT+TGG - chr1_1:11617319-11617338 Msa0008050:intron 45.0%
GACACTTGTGACATTGTGGT+TGG + chr1_1:11617345-11617364 None:intergenic 45.0%
TACACGTGTGACATTGTGGT+TGG - chr1_1:11617404-11617423 Msa0008050:intron 45.0%
!! GTTGGTGACATTGGTGACAT+TGG + chr1_1:11617430-11617449 None:intergenic 45.0%
!! GACATTGTGGTTGGTGACAT+TGG + chr1_1:11617319-11617338 None:intergenic 45.0%
TACTTGGGTGACATTGTGGT+TGG + chr1_1:11617448-11617467 None:intergenic 45.0%
GAAGATGGACCACATGTTAG+TGG + chr1_1:11617475-11617494 None:intergenic 45.0%
TAATAGCCACTCCATTAGGC+CGG + chr1_1:11617677-11617696 None:intergenic 45.0%
GCTCGTGCATGATGATTGTT+TGG - chr1_1:11617896-11617915 Msa0008050:CDS 45.0%
! TTTCTTGTTTGCGCCTCCAT+GGG + chr1_1:11618120-11618139 None:intergenic 45.0%
!! TTTTCTTGTTTGCGCCTCCA+TGG + chr1_1:11618121-11618140 None:intergenic 45.0%
CTCTTCCACCAACGTTAACG+CGG - chr1_1:11616142-11616161 Msa0008050:three_prime_UTR 50.0%
AAACACAGCCGCGTTAACGT+TGG + chr1_1:11616153-11616172 None:intergenic 50.0%
! AACGCGGCTGTGTTTTTCGT+CGG - chr1_1:11616158-11616177 Msa0008050:three_prime_UTR 50.0%
ATCGATATAGCCGTCGTTCG+CGG + chr1_1:11616201-11616220 None:intergenic 50.0%
GGTGCTTCCTCCCACTTAAA+CGG + chr1_1:11616225-11616244 None:intergenic 50.0%
!! TTTAAGTGGGAGGAAGCACC+AGG - chr1_1:11616225-11616244 Msa0008050:three_prime_UTR 50.0%
!! TTAAGTGGGAGGAAGCACCA+GGG - chr1_1:11616226-11616245 Msa0008050:three_prime_UTR 50.0%
GGTCGTGTATGACATGACCT+AGG + chr1_1:11616255-11616274 None:intergenic 50.0%
!! GAGCTTTGGAACTTCCTCCT+AGG - chr1_1:11616310-11616329 Msa0008050:exon 50.0%
!! GAGTGTTGATGGGGGTGATA+AGG - chr1_1:11616548-11616567 Msa0008050:intron 50.0%
AGAAGGGGAAGCTTCTCACA+TGG - chr1_1:11616591-11616610 Msa0008050:intron 50.0%
GCTGTGGTCCATCCCATTTA+TGG - chr1_1:11617128-11617147 Msa0008050:intron 50.0%
ACCAACATGGGGTAGCACTT+GGG - chr1_1:11617295-11617314 Msa0008050:intron 50.0%
GTAGCACTTGGGTGACATTG+TGG - chr1_1:11617306-11617325 Msa0008050:intron 50.0%
CACTTGGGTGACATTGTGGT+TGG - chr1_1:11617310-11617329 Msa0008050:intron 50.0%
TTAGGCCGGCCATGATAGTA+TGG + chr1_1:11617663-11617682 None:intergenic 50.0%
TACTATCATGGCCGGCCTAA+TGG - chr1_1:11617663-11617682 Msa0008050:intron 50.0%
! GCTTTAAGCAAGTGGTCCCA+TGG - chr1_1:11618101-11618120 Msa0008050:CDS 50.0%
TTAAGCAAGTGGTCCCATGG+AGG - chr1_1:11618104-11618123 Msa0008050:CDS 50.0%
TGACATGACCTAGGCTTCCC+TGG + chr1_1:11616246-11616265 None:intergenic 55.0%
GACACTGCACGTCCAACGTA+TGG + chr1_1:11616405-11616424 None:intergenic 55.0%
TTTGGGTCTAGTAGGTGGGG+TGG - chr1_1:11616471-11616490 Msa0008050:intron 55.0%
TTGGGTCTAGTAGGTGGGGT+GGG - chr1_1:11616472-11616491 Msa0008050:intron 55.0%
CACCAACATGGGGTAGCACT+TGG - chr1_1:11617294-11617313 Msa0008050:intron 55.0%
ACCCAAGTGCTACCCCATGT+TGG + chr1_1:11617299-11617318 None:intergenic 55.0%
CACAGCCGCGTTAACGTTGG+TGG + chr1_1:11616150-11616169 None:intergenic 60.0%
! GCGGCTGTGTTTTTCGTCGG+AGG - chr1_1:11616161-11616180 Msa0008050:three_prime_UTR 60.0%
AACTCCACCACCGCGAACGA+CGG - chr1_1:11616188-11616207 Msa0008050:three_prime_UTR 60.0%
GATATAGCCGTCGTTCGCGG+TGG + chr1_1:11616198-11616217 None:intergenic 60.0%
!! AGGAAGCACCAGGGAAGCCT+AGG - chr1_1:11616235-11616254 Msa0008050:three_prime_UTR 60.0%
TCATGGCCGGCCTAATGGAG+TGG - chr1_1:11617668-11617687 Msa0008050:intron 60.0%
!! GTCGTTCGCGGTGGTGGAGT+TGG + chr1_1:11616189-11616208 None:intergenic 65.0%
ATAGCCGTCGTTCGCGGTGG+TGG + chr1_1:11616195-11616214 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 11615971 11618506 11615971 ID=Msa0008050;Name=Msa0008050
chr1_1 mRNA 11615971 11618506 11615971 ID=Msa0008050-mRNA-1;Parent=Msa0008050;Name=Msa0008050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=92|1|1|1|1|1|2|353|211
chr1_1 exon 11615971 11616392 11615971 ID=Msa0008050-mRNA-1:exon:4286;Parent=Msa0008050-mRNA-1
chr1_1 exon 11617848 11618506 11617848 ID=Msa0008050-mRNA-1:exon:4285;Parent=Msa0008050-mRNA-1
chr1_1 five_prime_UTR 11618415 11618506 11618415 ID=Msa0008050-mRNA-1:five_prime_utr;Parent=Msa0008050-mRNA-1
chr1_1 CDS 11617848 11618414 11617848 ID=Msa0008050-mRNA-1:cds;Parent=Msa0008050-mRNA-1
chr1_1 CDS 11616324 11616392 11616324 ID=Msa0008050-mRNA-1:cds;Parent=Msa0008050-mRNA-1
chr1_1 three_prime_UTR 11615971 11616323 11615971 ID=Msa0008050-mRNA-1:three_prime_utr;Parent=Msa0008050-mRNA-1
Gene Sequence

>Msa0008050

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAACGTTAACGCGGCTGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCGATACCGTTTAAGTGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCATACACGACCAGAGCCAAAAGATAGAGAAGTTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCACCATCACCAACAACTGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACAAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGGGCAAGTATTTATGGAAGCTTTAAGCAAGTGGTCCCATGGAGGCGCAAACAAGAAAAACAACAAAATTGA
Protein sequence

>Msa0008050

MTSENNKKLDHDDDELRSNYNTPQKLSLPPTLTRLCFSSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKDREVNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLWASIYGSFKQVVPWRRKQEKQQN*