AlfalfaGEDB | Msa0057520

M. sativa cultivar zhongmu-4 / Msa0057520

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057520	XP_013465692.1	91.469	211	17	1	1	211	1	210	1.75e-135	388

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057520	A0A072VE41	91.469	211	17	1	1	211	1	210	8.35e-136	388

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa0057520	-0.815158	1.067028e-51	-8.615850e-47
Msa0004880	Msa0057520	0.818258	2.153572e-52	-8.615850e-47
Msa0007080	Msa0057520	0.806523	7.893550e-50	-8.615850e-47
Msa0008050	Msa0057520	0.814205	1.734389e-51	-8.615850e-47
Msa0011720	Msa0057520	0.823156	1.612141e-53	-8.615850e-47
Msa0012060	Msa0057520	0.806605	7.585513e-50	-8.615850e-47
Msa0014800	Msa0057520	0.802213	6.242887e-49	-8.615850e-47
Msa0026490	Msa0057520	0.814889	1.223973e-51	-8.615850e-47
Msa0033440	Msa0057520	0.816920	4.312156e-52	-8.615850e-47
Msa0037700	Msa0057520	0.823069	1.689270e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057520	MtrunA17_Chr1g0146921	91.469	211	17	1	1	211	1	210	1.61e-139	388
Msa0057520	MtrunA17_Chr3g0126981	58.382	173	57	5	39	210	3	161	2.31e-56	176
Msa0057520	MtrunA17_Chr8g0346691	35.000	180	66	10	43	192	35	193	8.92e-12	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057520	AT5G53030.1	40.099	202	76	10	45	207	40	235	8.41e-28	105
Msa0057520	AT5G53030.2	38.503	187	74	9	45	196	40	220	1.22e-21	89.4
Msa0057520	AT4G27810.1	38.202	178	68	8	45	207	36	186	2.66e-21	87.4

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Find 70 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCAAAACTACCCTCA+CGG	0.642581	1_2:-13612376	None:intergenic
AAAATAATAATATTAGCATA+TGG	0.462348	1_2:+13614136	Msa0057520:three_prime_UTR
AAACACGGTCGTGATAACGT+TGG	0.713578	1_2:-13612008	None:intergenic
AAATAATAGAATTGACCGTG+AGG	0.684752	1_2:+13612361	Msa0057520:CDS
AACAAGGGCAGTGTGAATTT+TGG	0.173203	1_2:+13612311	Msa0057520:CDS
AACTCCACCACCGCGAACGA+CGG	0.539846	1_2:+13612046	Msa0057520:CDS
AACTTATAAAACCGTAAATT+TGG	0.134308	1_2:-13613952	None:intergenic
AATAATAGAATTGACCGTGA+GGG	0.656653	1_2:+13612362	Msa0057520:CDS
AATATGAAAACATCAAAACT+AGG	0.601832	1_2:+13613928	Msa0057520:three_prime_UTR
ACATGGTACTTCACATTTGT+GGG	0.406088	1_2:+13612466	Msa0057520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTGGTGTTTATTTATAGA+GGG	-	chr1_2:13611836-13611855	None:intergenic	30.0%
TGTGTGGTGTTTATTTATAG+AGG	-	chr1_2:13611837-13611856	None:intergenic	30.0%
GTATAGGGATAGTAATAACA+AGG	+	chr1_2:13612295-13612314	Msa0057520:CDS	30.0%
AAATAATAGAATTGACCGTG+AGG	+	chr1_2:13612361-13612380	Msa0057520:CDS	30.0%
AATAATAGAATTGACCGTGA+GGG	+	chr1_2:13612362-13612381	Msa0057520:CDS	30.0%
GATTGTAGTTTCAAATGCTT+AGG	+	chr1_2:13612548-13612567	Msa0057520:intron	30.0%
TTAGAAAATGATTTGACCGA+TGG	+	chr1_2:13612648-13612667	Msa0057520:intron	30.0%
TAATCTAACTTCTACTCCAT+CGG	-	chr1_2:13612667-13612686	None:intergenic	30.0%
TTAATTTCATGACACTCTAG+GGG	-	chr1_2:13612819-13612838	None:intergenic	30.0%
ACAACAACAGTATATCAATC+GGG	-	chr1_2:13612939-13612958	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	13611829	13614186	13611829	ID=Msa0057520;Name=Msa0057520
chr1_2	mRNA	13611829	13614186	13611829	ID=Msa0057520-mRNA-1;Parent=Msa0057520;Name=Msa0057520-mRNA-1;_AED=0.01;_eAED=0.01;_QI=92\|1\|1\|1\|1\|1\|2\|353\|211
chr1_2	exon	13611829	13612487	13611829	ID=Msa0057520-mRNA-1:exon:5383;Parent=Msa0057520-mRNA-1
chr1_2	exon	13613765	13614186	13613765	ID=Msa0057520-mRNA-1:exon:5384;Parent=Msa0057520-mRNA-1
chr1_2	five_prime_UTR	13611829	13611920	13611829	ID=Msa0057520-mRNA-1:five_prime_utr;Parent=Msa0057520-mRNA-1
chr1_2	CDS	13611921	13612487	13611921	ID=Msa0057520-mRNA-1:cds;Parent=Msa0057520-mRNA-1
chr1_2	CDS	13613765	13613833	13613765	ID=Msa0057520-mRNA-1:cds;Parent=Msa0057520-mRNA-1
chr1_2	three_prime_UTR	13613834	13614186	13613834	ID=Msa0057520-mRNA-1:three_prime_utr;Parent=Msa0057520-mRNA-1

Gene Sequence

>Msa0057520

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAACGTTATCACGACCGTGTTTTTTGTCTGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCAATACCGTTTAAATGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCACACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCACCATCACCAACAACTGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACCAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGGGCAAGTATTTATGGAAGCTTTAAGCAAGTGGTCCCATGGAGGCGCAAACAAGAAAAACAACAAAATTGA

Protein sequence

>Msa0057520

MTSENNKKLDHDDDELRSNYNTPQKLSLPPTLSRPCFLSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLWASIYGSFKQVVPWRRKQEKQQN*