AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0113560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113560 MtrunA17_Chr1g0199571 90.244 41 4 0 42 82 129 169 3.50e-19 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113560 AT5G04220.3 63.415 41 15 0 42 82 67 107 8.85e-12 59.3
Msa0113560 AT5G04220.2 63.415 41 15 0 42 82 129 169 1.04e-11 59.3

Find 24 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGCAGGTATGAAAGTTT+TGG 0.273201 1_3:-29495285 Msa0113560:CDS
TGCAAGGTCAATACTATGTT+TGG 0.291324 1_3:+29495209 None:intergenic
GGAAACAAATGAAAAGGAAT+TGG 0.343840 1_3:-29495264 Msa0113560:CDS
TATTGCATTCCTTGCAATGA+TGG 0.437005 1_3:+29496003 None:intergenic
GCAATAAGACATTGGGATGA+TGG 0.439230 1_3:-29495986 Msa0113560:CDS
GCAAGGTCAATACTATGTTT+GGG 0.443699 1_3:+29495210 None:intergenic
TCATGAAACAAGTCATCAAA+TGG 0.463122 1_3:-29495241 Msa0113560:CDS
ATTGTTTGTAGCAATTCTGC+AGG 0.482743 1_3:-29495299 Msa0113560:intron
CAAGGAATGCAATAAGACAT+TGG 0.491395 1_3:-29495994 Msa0113560:CDS
AAGGAATGCAATAAGACATT+GGG 0.493762 1_3:-29495993 Msa0113560:CDS
TATGAGATTGAATTTATGTC+TGG 0.494249 1_3:-29496091 None:intergenic
CAATAAGACATTGGGATGAT+GGG 0.504343 1_3:-29495985 Msa0113560:intron
GTGTAGCGGAGGTAGGGAGG+TGG 0.510408 1_3:-29496069 Msa0113560:CDS
ATGTCTGGTGTAGCGGAGGT+AGG 0.526263 1_3:-29496076 Msa0113560:CDS
GTCTATGAAGATCAAAGTGC+AGG 0.534460 1_3:-29495180 Msa0113560:CDS
TGTAGCGGAGGTAGGGAGGT+GGG 0.547681 1_3:-29496068 Msa0113560:CDS
TGTCTGGTGTAGCGGAGGTA+GGG 0.549091 1_3:-29496075 Msa0113560:CDS
TGAATTTATGTCTGGTGTAG+CGG 0.559041 1_3:-29496083 None:intergenic
AAACAAGTCATCAAATGGAC+TGG 0.565498 1_3:-29495236 Msa0113560:CDS
CTGGTGTAGCGGAGGTAGGG+AGG 0.573553 1_3:-29496072 Msa0113560:CDS
ATTTATGTCTGGTGTAGCGG+AGG 0.638357 1_3:-29496080 None:intergenic
CTTCATAGACAACACATGCA+AGG 0.656238 1_3:+29495193 None:intergenic
TCTACTGCACCATCATTGCA+AGG 0.665556 1_3:-29496012 Msa0113560:CDS
ACACGCCGAAGAAAACTGTG+AGG 0.784742 1_3:+29496035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATGTTTTGTTTTT+AGG - chr1_3:29495537-29495556 Msa0113560:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr1_3:29495538-29495557 Msa0113560:intron 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG - chr1_3:29495537-29495556 Msa0113560:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr1_3:29495538-29495557 Msa0113560:intron 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG - chr1_3:29495537-29495556 Msa0113560:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr1_3:29495538-29495557 Msa0113560:intron 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG - chr1_3:29495537-29495556 Msa0113560:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr1_3:29495538-29495557 Msa0113560:intron 10.0%
!! AACATAATAATAAGAATGAT+TGG + chr1_3:29495612-29495631 None:intergenic 15.0%
!! AACATAATAATAAGAATGAT+TGG + chr1_3:29495612-29495631 None:intergenic 15.0%
!!! ACAAAATGATTTTGTGATAA+TGG - chr1_3:29495429-29495448 Msa0113560:intron 20.0%
!!! ACAAAATGATTTTGTGATAA+TGG - chr1_3:29495429-29495448 Msa0113560:intron 20.0%
! TCAATTAATTCAAAGTTGAG+AGG + chr1_3:29495297-29495316 None:intergenic 25.0%
! TTGCTAACTATAGAAAGAAA+TGG - chr1_3:29495379-29495398 Msa0113560:intron 25.0%
!!! TAAGTGTCATTTAGTGATAT+TGG - chr1_3:29495465-29495484 Msa0113560:intron 25.0%
!!! AAGTGTCATTTAGTGATATT+GGG - chr1_3:29495466-29495485 Msa0113560:intron 25.0%
! TGTAAAGCTTTCAAAATACA+AGG - chr1_3:29495793-29495812 Msa0113560:intron 25.0%
! TAATCCTCTCTTTCATTTAT+AGG - chr1_3:29495896-29495915 Msa0113560:intron 25.0%
! AATCCTCTCTTTCATTTATA+GGG - chr1_3:29495897-29495916 Msa0113560:intron 25.0%
!!! AGTTTTGGAAACAAATGAAA+AGG - chr1_3:29495972-29495991 Msa0113560:intron 25.0%
! TCAATTAATTCAAAGTTGAG+AGG + chr1_3:29495297-29495316 None:intergenic 25.0%
! TTGCTAACTATAGAAAGAAA+TGG - chr1_3:29495379-29495398 Msa0113560:intron 25.0%
!!! TAAGTGTCATTTAGTGATAT+TGG - chr1_3:29495465-29495484 Msa0113560:intron 25.0%
!!! AAGTGTCATTTAGTGATATT+GGG - chr1_3:29495466-29495485 Msa0113560:intron 25.0%
! TGTAAAGCTTTCAAAATACA+AGG - chr1_3:29495793-29495812 Msa0113560:intron 25.0%
! TAATCCTCTCTTTCATTTAT+AGG - chr1_3:29495896-29495915 Msa0113560:intron 25.0%
! AATCCTCTCTTTCATTTATA+GGG - chr1_3:29495897-29495916 Msa0113560:intron 25.0%
!!! AGTTTTGGAAACAAATGAAA+AGG - chr1_3:29495972-29495991 Msa0113560:intron 25.0%
AAGGAATGCAATAAGACATT+GGG - chr1_3:29495249-29495268 Msa0113560:CDS 30.0%
TCTAACTTTCATCACATTCA+TGG + chr1_3:29495340-29495359 None:intergenic 30.0%
TCTTTCTATAGTTAGCAAAG+TGG + chr1_3:29495377-29495396 None:intergenic 30.0%
AATGGTACGATAGATAAGTA+AGG - chr1_3:29495397-29495416 Msa0113560:intron 30.0%
ATGGTACGATAGATAAGTAA+GGG - chr1_3:29495398-29495417 Msa0113560:intron 30.0%
!! AGTGTCATTTAGTGATATTG+GGG - chr1_3:29495467-29495486 Msa0113560:intron 30.0%
AAGAATGAAAGTGATGATCA+TGG + chr1_3:29495518-29495537 None:intergenic 30.0%
TAGTGTTGTGATTCCTATTA+AGG - chr1_3:29495578-29495597 Msa0113560:intron 30.0%
AGTGTTGTGATTCCTATTAA+GGG - chr1_3:29495579-29495598 Msa0113560:intron 30.0%
! TAGTGTTGAGATTCCTATTA+AGG - chr1_3:29495661-29495680 Msa0113560:intron 30.0%
! AGTGTTGAGATTCCTATTAA+GGG - chr1_3:29495662-29495681 Msa0113560:intron 30.0%
!! CCACTTTAACTTTTGGATTT+TGG + chr1_3:29495695-29495714 None:intergenic 30.0%
CCAAAATCCAAAAGTTAAAG+TGG - chr1_3:29495692-29495711 Msa0113560:intron 30.0%
!!! AACAATGCCACTTTAACTTT+TGG + chr1_3:29495702-29495721 None:intergenic 30.0%
AAAAGTTAAAGTGGCATTGT+TGG - chr1_3:29495701-29495720 Msa0113560:intron 30.0%
! CTTTGTTTCTTTGATGTGTT+TGG - chr1_3:29495833-29495852 Msa0113560:intron 30.0%
ATCCTCTCTTTCATTTATAG+GGG - chr1_3:29495898-29495917 Msa0113560:intron 30.0%
GGAAACAAATGAAAAGGAAT+TGG - chr1_3:29495978-29495997 Msa0113560:intron 30.0%
TCATGAAACAAGTCATCAAA+TGG - chr1_3:29496001-29496020 Msa0113560:CDS 30.0%
AAGGAATGCAATAAGACATT+GGG - chr1_3:29495249-29495268 Msa0113560:CDS 30.0%
TCTAACTTTCATCACATTCA+TGG + chr1_3:29495340-29495359 None:intergenic 30.0%
TCTTTCTATAGTTAGCAAAG+TGG + chr1_3:29495377-29495396 None:intergenic 30.0%
AATGGTACGATAGATAAGTA+AGG - chr1_3:29495397-29495416 Msa0113560:intron 30.0%
ATGGTACGATAGATAAGTAA+GGG - chr1_3:29495398-29495417 Msa0113560:intron 30.0%
!! AGTGTCATTTAGTGATATTG+GGG - chr1_3:29495467-29495486 Msa0113560:intron 30.0%
AAGAATGAAAGTGATGATCA+TGG + chr1_3:29495518-29495537 None:intergenic 30.0%
TAGTGTTGTGATTCCTATTA+AGG - chr1_3:29495578-29495597 Msa0113560:intron 30.0%
AGTGTTGTGATTCCTATTAA+GGG - chr1_3:29495579-29495598 Msa0113560:intron 30.0%
! TAGTGTTGAGATTCCTATTA+AGG - chr1_3:29495661-29495680 Msa0113560:intron 30.0%
! AGTGTTGAGATTCCTATTAA+GGG - chr1_3:29495662-29495681 Msa0113560:intron 30.0%
!! CCACTTTAACTTTTGGATTT+TGG + chr1_3:29495695-29495714 None:intergenic 30.0%
CCAAAATCCAAAAGTTAAAG+TGG - chr1_3:29495692-29495711 Msa0113560:intron 30.0%
!!! AACAATGCCACTTTAACTTT+TGG + chr1_3:29495702-29495721 None:intergenic 30.0%
AAAAGTTAAAGTGGCATTGT+TGG - chr1_3:29495701-29495720 Msa0113560:intron 30.0%
! CTTTGTTTCTTTGATGTGTT+TGG - chr1_3:29495833-29495852 Msa0113560:intron 30.0%
ATCCTCTCTTTCATTTATAG+GGG - chr1_3:29495898-29495917 Msa0113560:intron 30.0%
GGAAACAAATGAAAAGGAAT+TGG - chr1_3:29495978-29495997 Msa0113560:intron 30.0%
TCATGAAACAAGTCATCAAA+TGG - chr1_3:29496001-29496020 Msa0113560:CDS 30.0%
TATTGCATTCCTTGCAATGA+TGG + chr1_3:29495242-29495261 None:intergenic 35.0%
CAAGGAATGCAATAAGACAT+TGG - chr1_3:29495248-29495267 Msa0113560:CDS 35.0%
CAATAAGACATTGGGATGAT+GGG - chr1_3:29495257-29495276 Msa0113560:CDS 35.0%
TCAAAGTTGAGAGGCTAAAT+TGG + chr1_3:29495288-29495307 None:intergenic 35.0%
GCTAAGATCAGCAAAATTTC+AGG - chr1_3:29495752-29495771 Msa0113560:intron 35.0%
ACAAAGCAACAAAATCACAG+AGG + chr1_3:29495819-29495838 None:intergenic 35.0%
TACCCCTATAAATGAAAGAG+AGG + chr1_3:29495903-29495922 None:intergenic 35.0%
ATTGTTTGTAGCAATTCTGC+AGG - chr1_3:29495943-29495962 Msa0113560:intron 35.0%
! TTCTGCAGGTATGAAAGTTT+TGG - chr1_3:29495957-29495976 Msa0113560:intron 35.0%
AAACAAGTCATCAAATGGAC+TGG - chr1_3:29496006-29496025 Msa0113560:CDS 35.0%
GCAAGGTCAATACTATGTTT+GGG + chr1_3:29496035-29496054 None:intergenic 35.0%
TGCAAGGTCAATACTATGTT+TGG + chr1_3:29496036-29496055 None:intergenic 35.0%
TATTGCATTCCTTGCAATGA+TGG + chr1_3:29495242-29495261 None:intergenic 35.0%
CAAGGAATGCAATAAGACAT+TGG - chr1_3:29495248-29495267 Msa0113560:CDS 35.0%
CAATAAGACATTGGGATGAT+GGG - chr1_3:29495257-29495276 Msa0113560:CDS 35.0%
TCAAAGTTGAGAGGCTAAAT+TGG + chr1_3:29495288-29495307 None:intergenic 35.0%
GCTAAGATCAGCAAAATTTC+AGG - chr1_3:29495752-29495771 Msa0113560:intron 35.0%
ACAAAGCAACAAAATCACAG+AGG + chr1_3:29495819-29495838 None:intergenic 35.0%
TACCCCTATAAATGAAAGAG+AGG + chr1_3:29495903-29495922 None:intergenic 35.0%
ATTGTTTGTAGCAATTCTGC+AGG - chr1_3:29495943-29495962 Msa0113560:intron 35.0%
! TTCTGCAGGTATGAAAGTTT+TGG - chr1_3:29495957-29495976 Msa0113560:intron 35.0%
AAACAAGTCATCAAATGGAC+TGG - chr1_3:29496006-29496025 Msa0113560:CDS 35.0%
GCAAGGTCAATACTATGTTT+GGG + chr1_3:29496035-29496054 None:intergenic 35.0%
TGCAAGGTCAATACTATGTT+TGG + chr1_3:29496036-29496055 None:intergenic 35.0%
! GCAATCCTCACAGTTTTCTT+CGG - chr1_3:29495202-29495221 Msa0113560:CDS 40.0%
GCAATAAGACATTGGGATGA+TGG - chr1_3:29495256-29495275 Msa0113560:CDS 40.0%
GTCATTTAGTGATATTGGGG+TGG - chr1_3:29495470-29495489 Msa0113560:intron 40.0%
ATTGGAGCAACACCCTTAAT+AGG + chr1_3:29495594-29495613 None:intergenic 40.0%
! TTTGGAGCAACACCCTTAAT+AGG + chr1_3:29495677-29495696 None:intergenic 40.0%
GTGAACATAAGCGAAATGCA+AGG - chr1_3:29495728-29495747 Msa0113560:intron 40.0%
CTTCATAGACAACACATGCA+AGG + chr1_3:29496052-29496071 None:intergenic 40.0%
GTCTATGAAGATCAAAGTGC+AGG - chr1_3:29496062-29496081 Msa0113560:CDS 40.0%
! GCAATCCTCACAGTTTTCTT+CGG - chr1_3:29495202-29495221 Msa0113560:CDS 40.0%
GCAATAAGACATTGGGATGA+TGG - chr1_3:29495256-29495275 Msa0113560:CDS 40.0%
GTCATTTAGTGATATTGGGG+TGG - chr1_3:29495470-29495489 Msa0113560:intron 40.0%
ATTGGAGCAACACCCTTAAT+AGG + chr1_3:29495594-29495613 None:intergenic 40.0%
! TTTGGAGCAACACCCTTAAT+AGG + chr1_3:29495677-29495696 None:intergenic 40.0%
GTGAACATAAGCGAAATGCA+AGG - chr1_3:29495728-29495747 Msa0113560:intron 40.0%
CTTCATAGACAACACATGCA+AGG + chr1_3:29496052-29496071 None:intergenic 40.0%
GTCTATGAAGATCAAAGTGC+AGG - chr1_3:29496062-29496081 Msa0113560:CDS 40.0%
TCTACTGCACCATCATTGCA+AGG - chr1_3:29495230-29495249 Msa0113560:CDS 45.0%
!! AGACATTGGGATGATGGGTT+TGG - chr1_3:29495262-29495281 Msa0113560:CDS 45.0%
TCTACTGCACCATCATTGCA+AGG - chr1_3:29495230-29495249 Msa0113560:CDS 45.0%
!! AGACATTGGGATGATGGGTT+TGG - chr1_3:29495262-29495281 Msa0113560:CDS 45.0%
ACACGCCGAAGAAAACTGTG+AGG + chr1_3:29495210-29495229 None:intergenic 50.0%
ACACGCCGAAGAAAACTGTG+AGG + chr1_3:29495210-29495229 None:intergenic 50.0%
! ATGTCTGGTGTAGCGGAGGT+AGG - chr1_3:29495166-29495185 Msa0113560:exon 55.0%
! TGTCTGGTGTAGCGGAGGTA+GGG - chr1_3:29495167-29495186 Msa0113560:exon 55.0%
! ATGTCTGGTGTAGCGGAGGT+AGG - chr1_3:29495166-29495185 Msa0113560:exon 55.0%
! TGTCTGGTGTAGCGGAGGTA+GGG - chr1_3:29495167-29495186 Msa0113560:exon 55.0%
! TGTAGCGGAGGTAGGGAGGT+GGG - chr1_3:29495174-29495193 Msa0113560:CDS 60.0%
! TGTAGCGGAGGTAGGGAGGT+GGG - chr1_3:29495174-29495193 Msa0113560:CDS 60.0%
! CTGGTGTAGCGGAGGTAGGG+AGG - chr1_3:29495170-29495189 Msa0113560:exon 65.0%
! GTGTAGCGGAGGTAGGGAGG+TGG - chr1_3:29495173-29495192 Msa0113560:CDS 65.0%
! CTGGTGTAGCGGAGGTAGGG+AGG - chr1_3:29495170-29495189 Msa0113560:exon 65.0%
! GTGTAGCGGAGGTAGGGAGG+TGG - chr1_3:29495173-29495192 Msa0113560:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 29495166 29496098 29495166 ID=Msa0113560;Name=Msa0113560
chr1_3 mRNA 29495166 29496098 29495166 ID=Msa0113560-mRNA-1;Parent=Msa0113560;Name=Msa0113560-mRNA-1;_AED=0.36;_eAED=0.63;_QI=0|0|0.5|1|1|1|2|6|83
chr1_3 exon 29495166 29495310 29495166 ID=Msa0113560-mRNA-1:exon:9805;Parent=Msa0113560-mRNA-1
chr1_3 exon 29495986 29496098 29495986 ID=Msa0113560-mRNA-1:exon:9804;Parent=Msa0113560-mRNA-1
chr1_3 CDS 29495986 29496098 29495986 ID=Msa0113560-mRNA-1:cds;Parent=Msa0113560-mRNA-1
chr1_3 CDS 29495172 29495310 29495172 ID=Msa0113560-mRNA-1:cds;Parent=Msa0113560-mRNA-1
chr1_3 three_prime_UTR 29495166 29495171 29495166 ID=Msa0113560-mRNA-1:three_prime_utr;Parent=Msa0113560-mRNA-1
Gene Sequence

>Msa0113560

ATGTCTGGTGTAGCGGAGGTAGGGAGGTGGGTTGTTGCAATCCTCACAGTTTTCTTCGGCGTGTTCTACTGCACCATCATTGCAAGGAATGCAATAAGACATTGGGATGATGGCAATTCTGCAGGTATGAAAGTTTTGGAAACAAATGAAAAGGAATTGGTCATGAAACAAGTCATCAAATGGACTGGCAACCCAAACATAGTATTGACCTTGCATGTGTTGTCTATGAAGATCAAAGTGCAGGTCTGTTAA

Protein sequence

>Msa0113560

MSGVAEVGRWVVAILTVFFGVFYCTIIARNAIRHWDDGNSAGMKVLETNEKELVMKQVIKWTGNPNIVLTLHVLSMKIKVQVC*