AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0148310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148310 MtrunA17_Chr1g0150751 95.853 217 7 2 1 216 24 239 3.52e-132 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCGCCTTCTTCGGGTTC+AGG 0.278379 1_4:+8562146 Msa0148310:CDS
TGAGGCATAATAATAGTAAT+AGG 0.283637 1_4:-8562219 None:intergenic
TTTGAAATCTTCGCCTTCTT+CGG 0.306660 1_4:+8562139 Msa0148310:CDS
CAAGGACAACCTAAAGTAAT+TGG 0.337399 1_4:+8562170 Msa0148310:CDS
TTGAAATCTTCGCCTTCTTC+GGG 0.338296 1_4:+8562140 Msa0148310:CDS
GAATTTGATGAAGCAAGTTT+AGG 0.338870 1_4:-8561798 None:intergenic
AGTCCTTCGTCACATTCTTC+CGG 0.348481 1_4:+8562098 Msa0148310:CDS
GTCCTTCGTCACATTCTTCC+GGG 0.349605 1_4:+8562099 Msa0148310:CDS
GGCGGTCCACCAATTACTTT+AGG 0.371369 1_4:-8562179 None:intergenic
TTTAGAAGTGGAGGAGGAGG+AGG 0.374484 1_4:-8562008 None:intergenic
TTTGATGATGTGAGTGGAAA+TGG 0.378894 1_4:-8561981 None:intergenic
TTCATCAGATATTCTTTGCT+TGG 0.384427 1_4:+8562297 Msa0148310:CDS
TAATAATAGTAATAGGGATA+TGG 0.384788 1_4:-8562212 None:intergenic
TGTGTTAGGAGTGGAGTAGA+TGG 0.387212 1_4:-8561918 None:intergenic
GTTAATAGTGGAGGAGGTGG+AGG 0.430557 1_4:-8561945 None:intergenic
TGGGGTGTTATTAGTGGAAA+AGG 0.432885 1_4:-8561891 None:intergenic
TTGATGATGTGAGTGGAAAT+GGG 0.439050 1_4:-8561980 None:intergenic
CCTTCTTCGGGTTCAGGTCA+AGG 0.439335 1_4:+8562152 Msa0148310:CDS
GGTGAATATGATGTTAATAG+TGG 0.445887 1_4:-8561957 None:intergenic
GAGGCATAATAATAGTAATA+GGG 0.446723 1_4:-8562218 None:intergenic
AGTGGAGTAGATGGAGGGTG+GGG 0.452281 1_4:-8561909 None:intergenic
GGAGGGTGGGGTGTTATTAG+TGG 0.452753 1_4:-8561897 None:intergenic
ATGCATAATAAACCACTTGA+AGG 0.460999 1_4:-8561732 None:intergenic
GGGGTGTTATTAGTGGAAAA+GGG 0.461571 1_4:-8561890 None:intergenic
GATGTTAATAGTGGAGGAGG+TGG 0.484497 1_4:-8561948 None:intergenic
AAAGAGAACATTGGAGATTG+AGG 0.496507 1_4:+8561692 Msa0148310:CDS
GGGTGTTATTAGTGGAAAAG+GGG 0.496788 1_4:-8561889 None:intergenic
GAGTGGAGTAGATGGAGGGT+GGG 0.505478 1_4:-8561910 None:intergenic
GAAGAATGTGACGAAGGACT+TGG 0.508990 1_4:-8562095 None:intergenic
GGCGAAGATTTCAAAGGTGG+TGG 0.511485 1_4:-8562131 None:intergenic
GGAGTGGAGTAGATGGAGGG+TGG 0.519473 1_4:-8561911 None:intergenic
TTTGTTTGCAAAGAGAACAT+TGG 0.519949 1_4:+8561683 Msa0148310:CDS
GGTGGTGGTGAACAAATTGT+AGG 0.526245 1_4:-8561774 None:intergenic
GTCAACAAAGGAGGAGGTGA+AGG 0.526752 1_4:-8561855 None:intergenic
TTTGATGAAGCAAGTTTAGG+AGG 0.539473 1_4:-8561795 None:intergenic
GATGAAGCAAGTTTAGGAGG+TGG 0.545014 1_4:-8561792 None:intergenic
GAAGATTTCAAAGGTGGTGG+AGG 0.554576 1_4:-8562128 None:intergenic
GAATGTGACGAAGGACTTGG+TGG 0.555897 1_4:-8562092 None:intergenic
CCTTGACCTGAACCCGAAGA+AGG 0.567300 1_4:-8562152 None:intergenic
TATGATGTTAATAGTGGAGG+AGG 0.568744 1_4:-8561951 None:intergenic
GGACAACCTAAAGTAATTGG+TGG 0.583322 1_4:+8562173 Msa0148310:CDS
GAAGCAAGTTTAGGAGGTGG+TGG 0.584772 1_4:-8561789 None:intergenic
TGATGATGTGAGTGGAAATG+GGG 0.585770 1_4:-8561979 None:intergenic
AAAGTTGAAGAGCCTTCAAG+TGG 0.589794 1_4:+8561720 Msa0148310:CDS
AGGTGGTGGAGGAGTTTCCC+CGG 0.592101 1_4:-8562117 None:intergenic
GTGGTGGAAGACTTAGTGAT+GGG 0.594811 1_4:-8562043 None:intergenic
GTTAGGAGTGGAGTAGATGG+AGG 0.597422 1_4:-8561915 None:intergenic
TTAGGAGTGGAGTAGATGGA+GGG 0.598597 1_4:-8561914 None:intergenic
TTTGGTGTCAACAAAGGAGG+AGG 0.602071 1_4:-8561861 None:intergenic
GGTGCATTTGGTGTCAACAA+AGG 0.602133 1_4:-8561867 None:intergenic
GAAGGACTTGGTGGAGATGG+TGG 0.605778 1_4:-8562083 None:intergenic
GGTGGAAGACTTAGTGATGG+GGG 0.608316 1_4:-8562041 None:intergenic
GACGAAGGACTTGGTGGAGA+TGG 0.610720 1_4:-8562086 None:intergenic
GAATATGATGTTAATAGTGG+AGG 0.611804 1_4:-8561954 None:intergenic
GGTAGAGTCAACAATGGTGG+TGG 0.613265 1_4:-8562059 None:intergenic
GGACTTGGTGGAGATGGTGG+CGG 0.623426 1_4:-8562080 None:intergenic
GGAGAATTTGATGATGTGAG+TGG 0.631413 1_4:-8561987 None:intergenic
GCATTTGGTGTCAACAAAGG+AGG 0.639121 1_4:-8561864 None:intergenic
TCCTTCGTCACATTCTTCCG+GGG 0.641768 1_4:+8562100 Msa0148310:CDS
TGGTGGAAGACTTAGTGATG+GGG 0.649103 1_4:-8562042 None:intergenic
GAAGAAGGCGAAGATTTCAA+AGG 0.649156 1_4:-8562137 None:intergenic
GAAGGCGAAGATTTCAAAGG+TGG 0.657373 1_4:-8562134 None:intergenic
GGCGGTAGAGTCAACAATGG+TGG 0.660549 1_4:-8562062 None:intergenic
GGTGGTGGAAGACTTAGTGA+TGG 0.666350 1_4:-8562044 None:intergenic
GGTGTTATTAGTGGAAAAGG+GGG 0.675151 1_4:-8561888 None:intergenic
TCCCCGGAAGAATGTGACGA+AGG 0.679405 1_4:-8562101 None:intergenic
GATGATGTGAGTGGAAATGG+GGG 0.687997 1_4:-8561978 None:intergenic
GGTGGCGGTAGAGTCAACAA+TGG 0.730976 1_4:-8562065 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAGTAATAGGGATA+TGG - chr1_4:8562215-8562234 None:intergenic 20.0%
! GAGGCATAATAATAGTAATA+GGG - chr1_4:8562221-8562240 None:intergenic 25.0%
! TGAGGCATAATAATAGTAAT+AGG - chr1_4:8562222-8562241 None:intergenic 25.0%
TTTGTTTGCAAAGAGAACAT+TGG + chr1_4:8561683-8561702 Msa0148310:CDS 30.0%
ATGCATAATAAACCACTTGA+AGG - chr1_4:8561735-8561754 None:intergenic 30.0%
GAATTTGATGAAGCAAGTTT+AGG - chr1_4:8561801-8561820 None:intergenic 30.0%
GAATATGATGTTAATAGTGG+AGG - chr1_4:8561957-8561976 None:intergenic 30.0%
GGTGAATATGATGTTAATAG+TGG - chr1_4:8561960-8561979 None:intergenic 30.0%
TTCATCAGATATTCTTTGCT+TGG + chr1_4:8562297-8562316 Msa0148310:CDS 30.0%
AAAGAGAACATTGGAGATTG+AGG + chr1_4:8561692-8561711 Msa0148310:CDS 35.0%
TTTGATGAAGCAAGTTTAGG+AGG - chr1_4:8561798-8561817 None:intergenic 35.0%
! TATGATGTTAATAGTGGAGG+AGG - chr1_4:8561954-8561973 None:intergenic 35.0%
!! TTGATGATGTGAGTGGAAAT+GGG - chr1_4:8561983-8562002 None:intergenic 35.0%
!! TTTGATGATGTGAGTGGAAA+TGG - chr1_4:8561984-8562003 None:intergenic 35.0%
!! TATGATTTTAGAAGTGGAGG+AGG - chr1_4:8562017-8562036 None:intergenic 35.0%
!! GGATATGATTTTAGAAGTGG+AGG - chr1_4:8562020-8562039 None:intergenic 35.0%
!! GGAGGATATGATTTTAGAAG+TGG - chr1_4:8562023-8562042 None:intergenic 35.0%
! TTTGAAATCTTCGCCTTCTT+CGG + chr1_4:8562139-8562158 Msa0148310:CDS 35.0%
CAAGGACAACCTAAAGTAAT+TGG + chr1_4:8562170-8562189 Msa0148310:CDS 35.0%
!! TAGGGATATGGATAGTTTTG+TGG - chr1_4:8562203-8562222 None:intergenic 35.0%
AGAAAAATGAGATGCACTTG+AGG - chr1_4:8562240-8562259 None:intergenic 35.0%
AAAGTTGAAGAGCCTTCAAG+TGG + chr1_4:8561720-8561739 Msa0148310:CDS 40.0%
! AGGTGAAGGATTTTGTGTTG+GGG - chr1_4:8561844-8561863 None:intergenic 40.0%
! GAGGTGAAGGATTTTGTGTT+GGG - chr1_4:8561845-8561864 None:intergenic 40.0%
!! GGTGTTATTAGTGGAAAAGG+GGG - chr1_4:8561891-8561910 None:intergenic 40.0%
!! GGGTGTTATTAGTGGAAAAG+GGG - chr1_4:8561892-8561911 None:intergenic 40.0%
!! GGGGTGTTATTAGTGGAAAA+GGG - chr1_4:8561893-8561912 None:intergenic 40.0%
!! TGGGGTGTTATTAGTGGAAA+AGG - chr1_4:8561894-8561913 None:intergenic 40.0%
! TGATGATGTGAGTGGAAATG+GGG - chr1_4:8561982-8562001 None:intergenic 40.0%
!! GGAGAATTTGATGATGTGAG+TGG - chr1_4:8561990-8562009 None:intergenic 40.0%
GAAGAAGGCGAAGATTTCAA+AGG - chr1_4:8562140-8562159 None:intergenic 40.0%
TTGAAATCTTCGCCTTCTTC+GGG + chr1_4:8562140-8562159 Msa0148310:CDS 40.0%
GGACAACCTAAAGTAATTGG+TGG + chr1_4:8562173-8562192 Msa0148310:CDS 40.0%
!!! GGATATGGATAGTTTTGTGG+CGG - chr1_4:8562200-8562219 None:intergenic 40.0%
! GGTGGTGGTGAACAAATTGT+AGG - chr1_4:8561777-8561796 None:intergenic 45.0%
GATGAAGCAAGTTTAGGAGG+TGG - chr1_4:8561795-8561814 None:intergenic 45.0%
! GGTGAAGGATTTTGTGTTGG+GGG - chr1_4:8561843-8561862 None:intergenic 45.0%
! GGAGGTGAAGGATTTTGTGT+TGG - chr1_4:8561846-8561865 None:intergenic 45.0%
!! TTTGGTGTCAACAAAGGAGG+AGG - chr1_4:8561864-8561883 None:intergenic 45.0%
!! GCATTTGGTGTCAACAAAGG+AGG - chr1_4:8561867-8561886 None:intergenic 45.0%
!! GGTGCATTTGGTGTCAACAA+AGG - chr1_4:8561870-8561889 None:intergenic 45.0%
TTAGGAGTGGAGTAGATGGA+GGG - chr1_4:8561917-8561936 None:intergenic 45.0%
TGTGTTAGGAGTGGAGTAGA+TGG - chr1_4:8561921-8561940 None:intergenic 45.0%
! GGAGGATTTTGTGTTAGGAG+TGG - chr1_4:8561930-8561949 None:intergenic 45.0%
! GAGGTGGAGGATTTTGTGTT+AGG - chr1_4:8561935-8561954 None:intergenic 45.0%
! GATGTTAATAGTGGAGGAGG+TGG - chr1_4:8561951-8561970 None:intergenic 45.0%
GATGATGTGAGTGGAAATGG+GGG - chr1_4:8561981-8562000 None:intergenic 45.0%
!! GATTTTAGAAGTGGAGGAGG+AGG - chr1_4:8562014-8562033 None:intergenic 45.0%
TGGTGGAAGACTTAGTGATG+GGG - chr1_4:8562045-8562064 None:intergenic 45.0%
! GTGGTGGAAGACTTAGTGAT+GGG - chr1_4:8562046-8562065 None:intergenic 45.0%
GAAGAATGTGACGAAGGACT+TGG - chr1_4:8562098-8562117 None:intergenic 45.0%
AGTCCTTCGTCACATTCTTC+CGG + chr1_4:8562098-8562117 Msa0148310:CDS 45.0%
! GAAGATTTCAAAGGTGGTGG+AGG - chr1_4:8562131-8562150 None:intergenic 45.0%
GAAGGCGAAGATTTCAAAGG+TGG - chr1_4:8562137-8562156 None:intergenic 45.0%
GAAGCAAGTTTAGGAGGTGG+TGG - chr1_4:8561792-8561811 None:intergenic 50.0%
! TTTTGTGTTGGGGGTGAAGG+AGG - chr1_4:8561834-8561853 None:intergenic 50.0%
! GGATTTTGTGTTGGGGGTGA+AGG - chr1_4:8561837-8561856 None:intergenic 50.0%
GTCAACAAAGGAGGAGGTGA+AGG - chr1_4:8561858-8561877 None:intergenic 50.0%
! AGTGGAAAAGGGGGTGCATT+TGG - chr1_4:8561882-8561901 None:intergenic 50.0%
GTTAGGAGTGGAGTAGATGG+AGG - chr1_4:8561918-8561937 None:intergenic 50.0%
! GTTAATAGTGGAGGAGGTGG+AGG - chr1_4:8561948-8561967 None:intergenic 50.0%
TTTAGAAGTGGAGGAGGAGG+AGG - chr1_4:8562011-8562030 None:intergenic 50.0%
GGTGGAAGACTTAGTGATGG+GGG - chr1_4:8562044-8562063 None:intergenic 50.0%
!! GGTGGTGGAAGACTTAGTGA+TGG - chr1_4:8562047-8562066 None:intergenic 50.0%
GGTAGAGTCAACAATGGTGG+TGG - chr1_4:8562062-8562081 None:intergenic 50.0%
GAATGTGACGAAGGACTTGG+TGG - chr1_4:8562095-8562114 None:intergenic 50.0%
GTCCTTCGTCACATTCTTCC+GGG + chr1_4:8562099-8562118 Msa0148310:CDS 50.0%
TCCTTCGTCACATTCTTCCG+GGG + chr1_4:8562100-8562119 Msa0148310:CDS 50.0%
GGCGAAGATTTCAAAGGTGG+TGG - chr1_4:8562134-8562153 None:intergenic 50.0%
GGCGGTCCACCAATTACTTT+AGG - chr1_4:8562182-8562201 None:intergenic 50.0%
!! GGAGGGTGGGGTGTTATTAG+TGG - chr1_4:8561900-8561919 None:intergenic 55.0%
AGTGGAGTAGATGGAGGGTG+GGG - chr1_4:8561912-8561931 None:intergenic 55.0%
GAGTGGAGTAGATGGAGGGT+GGG - chr1_4:8561913-8561932 None:intergenic 55.0%
! GGAAGACTTAGTGATGGGGG+AGG - chr1_4:8562041-8562060 None:intergenic 55.0%
GGCGGTAGAGTCAACAATGG+TGG - chr1_4:8562065-8562084 None:intergenic 55.0%
! GGTGGCGGTAGAGTCAACAA+TGG - chr1_4:8562068-8562087 None:intergenic 55.0%
!! GAAGGACTTGGTGGAGATGG+TGG - chr1_4:8562086-8562105 None:intergenic 55.0%
!! GACGAAGGACTTGGTGGAGA+TGG - chr1_4:8562089-8562108 None:intergenic 55.0%
TCCCCGGAAGAATGTGACGA+AGG - chr1_4:8562104-8562123 None:intergenic 55.0%
! TCTTCGCCTTCTTCGGGTTC+AGG + chr1_4:8562146-8562165 Msa0148310:CDS 55.0%
CCTTGACCTGAACCCGAAGA+AGG - chr1_4:8562155-8562174 None:intergenic 55.0%
! CCTTCTTCGGGTTCAGGTCA+AGG + chr1_4:8562152-8562171 Msa0148310:CDS 55.0%
GTGTTGGGGGTGAAGGAGGA+GGG - chr1_4:8561830-8561849 None:intergenic 60.0%
TGTGTTGGGGGTGAAGGAGG+AGG - chr1_4:8561831-8561850 None:intergenic 60.0%
GGAGTGGAGTAGATGGAGGG+TGG - chr1_4:8561914-8561933 None:intergenic 60.0%
!! GGACTTGGTGGAGATGGTGG+CGG - chr1_4:8562083-8562102 None:intergenic 60.0%
!! AGGTGGTGGAGGAGTTTCCC+CGG - chr1_4:8562120-8562139 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 8561672 8562322 8561672 ID=Msa0148310;Name=Msa0148310
chr1_4 mRNA 8561672 8562322 8561672 ID=Msa0148310-mRNA-1;Parent=Msa0148310;Name=Msa0148310-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|216
chr1_4 exon 8561672 8562322 8561672 ID=Msa0148310-mRNA-1:exon:3653;Parent=Msa0148310-mRNA-1
chr1_4 CDS 8561672 8562322 8561672 ID=Msa0148310-mRNA-1:cds;Parent=Msa0148310-mRNA-1
Gene Sequence

>Msa0148310

ATGTTGTTGGTTTTGTTTGCAAAGAGAACATTGGAGATTGAGGACAACAAAGTTGAAGAGCCTTCAAGTGGTTTATTATGCATTAGTGAATGTGTCACGTGTCCTACAATTTGTTCACCACCACCTCCTAAACTTGCTTCATCAAATTCAAATCACCCTCCTCCTTCACCCCCAACACAAAATCCTTCACCTCCTCCTTTGTTGACACCAAATGCACCCCCTTTTCCACTAATAACACCCCACCCTCCATCTACTCCACTCCTAACACAAAATCCTCCACCTCCTCCACTATTAACATCATATTCACCCCCATTTCCACTCACATCATCAAATTCTCCTCCTCCTCCTCCACTTCTAAAATCATATCCTCCCCCATCACTAAGTCTTCCACCACCATTGTTGACTCTACCGCCACCATCTCCACCAAGTCCTTCGTCACATTCTTCCGGGGAAACTCCTCCACCACCTTTGAAATCTTCGCCTTCTTCGGGTTCAGGTCAAGGACAACCTAAAGTAATTGGTGGACCGCCACAAAACTATCCATATCCCTATTACTATTATTATGCCTCAAGTGCATCTCATTTTTCTAATCATGTTGTACTCTTCTTTATTGTACTTTTGTTCATTCATCAGATATTCTTTGCTTGGTAA

Protein sequence

>Msa0148310

MLLVLFAKRTLEIEDNKVEEPSSGLLCISECVTCPTICSPPPPKLASSNSNHPPPSPPTQNPSPPPLLTPNAPPFPLITPHPPSTPLLTQNPPPPPLLTSYSPPFPLTSSNSPPPPPLLKSYPPPSLSLPPPLLTLPPPSPPSPSSHSSGETPPPPLKSSPSSGSGQGQPKVIGGPPQNYPYPYYYYYASSASHFSNHVVLFFIVLLFIHQIFFAW*