AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0005390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005390 MtrunA17_Chr1g0150751 86.667 240 30 2 1 239 1 239 2.45e-94 276
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCGCCTTCTTCGGGTTC+AGG 0.278379 1_1:-7892908 Msa0005390:CDS
TGAGGCATAATAATAGTAAT+AGG 0.283637 1_1:+7892835 None:intergenic
TTTGAAATCTTCGCCTTCTT+CGG 0.306660 1_1:-7892915 Msa0005390:CDS
GGTGGTCCACCAATTACTTT+AGG 0.326407 1_1:+7892875 None:intergenic
CAAGGACAACCTAAAGTAAT+TGG 0.337399 1_1:-7892884 Msa0005390:CDS
TTGAAATCTTCGCCTTCTTC+GGG 0.338296 1_1:-7892914 Msa0005390:CDS
GAATTTGATGAAGCAAGTTT+AGG 0.338870 1_1:+7893256 None:intergenic
AGTCCTTCGTCACATTCTTC+CGG 0.348481 1_1:-7892956 Msa0005390:CDS
GTCCTTCGTCACATTCTTCC+GGG 0.349605 1_1:-7892955 Msa0005390:CDS
TTTGATGATGTGAGTGGAAA+TGG 0.378894 1_1:+7893073 None:intergenic
TTCATCAGATATTCTTTGCT+TGG 0.384427 1_1:-7892757 Msa0005390:CDS
TAATAATAGTAATAGGGATA+TGG 0.384788 1_1:+7892842 None:intergenic
TGTGTTAGGAGTGGAGTAGA+TGG 0.387212 1_1:+7893136 None:intergenic
TTTAGAAGTGAAGGAGGAGG+AGG 0.388578 1_1:+7893046 None:intergenic
GTTAATAGTGGAGGAGGTGG+AGG 0.430557 1_1:+7893109 None:intergenic
TGGGGTGTTATTAGTGGAAA+AGG 0.432885 1_1:+7893163 None:intergenic
ATGGGTCATAACAAGAATCT+AGG 0.435363 1_1:-7893451 Msa0005390:CDS
TTGATGATGTGAGTGGAAAT+GGG 0.439050 1_1:+7893074 None:intergenic
CCTTCTTCGGGTTCAGGTCA+AGG 0.439335 1_1:-7892902 Msa0005390:CDS
GGTGAATATGATGTTAATAG+TGG 0.445887 1_1:+7893097 None:intergenic
GAGGCATAATAATAGTAATA+GGG 0.446723 1_1:+7892836 None:intergenic
AGTGGAGTAGATGGAGGGTG+GGG 0.452281 1_1:+7893145 None:intergenic
GGAGGGTGGGGTGTTATTAG+TGG 0.452753 1_1:+7893157 None:intergenic
ATGCATAATAAACCACTTGA+AGG 0.460999 1_1:+7893322 None:intergenic
GGGGTGTTATTAGTGGAAAA+GGG 0.461571 1_1:+7893164 None:intergenic
GATGTTAATAGTGGAGGAGG+TGG 0.484497 1_1:+7893106 None:intergenic
AAAGAGAACATTGGAGATTG+AGG 0.496507 1_1:-7893362 Msa0005390:CDS
GGGTGTTATTAGTGGAAAAG+GGG 0.496788 1_1:+7893165 None:intergenic
GAGTGGAGTAGATGGAGGGT+GGG 0.505478 1_1:+7893144 None:intergenic
GAAGAATGTGACGAAGGACT+TGG 0.508990 1_1:+7892959 None:intergenic
GGCGAAGATTTCAAAGGTGG+TGG 0.511485 1_1:+7892923 None:intergenic
GGAGTGGAGTAGATGGAGGG+TGG 0.519473 1_1:+7893143 None:intergenic
TTTGTTTGCAAAGAGAACAT+TGG 0.519949 1_1:-7893371 Msa0005390:CDS
GGTGGTGGTGAACAAATTGT+AGG 0.526245 1_1:+7893280 None:intergenic
GTCAACAAAGGAGGAGGTGA+AGG 0.526752 1_1:+7893199 None:intergenic
TTTGATGAAGCAAGTTTAGG+AGG 0.539473 1_1:+7893259 None:intergenic
GATGAAGCAAGTTTAGGAGG+TGG 0.545014 1_1:+7893262 None:intergenic
GAAGATTTCAAAGGTGGTGG+AGG 0.554576 1_1:+7892926 None:intergenic
GAATGTGACGAAGGACTTGG+TGG 0.555897 1_1:+7892962 None:intergenic
CCTTGACCTGAACCCGAAGA+AGG 0.567300 1_1:+7892902 None:intergenic
TATGATGTTAATAGTGGAGG+AGG 0.568744 1_1:+7893103 None:intergenic
GGACAACCTAAAGTAATTGG+TGG 0.583322 1_1:-7892881 Msa0005390:CDS
GAAGCAAGTTTAGGAGGTGG+TGG 0.584772 1_1:+7893265 None:intergenic
TGATGATGTGAGTGGAAATG+GGG 0.585770 1_1:+7893075 None:intergenic
AAAGTTGAAGAGCCTTCAAG+TGG 0.589794 1_1:-7893334 Msa0005390:CDS
AGGTGGTGGAGGAGTTTCCC+CGG 0.592101 1_1:+7892937 None:intergenic
GTGGTGGAAGACTTAGTGAT+GGG 0.594811 1_1:+7893011 None:intergenic
GTTAGGAGTGGAGTAGATGG+AGG 0.597422 1_1:+7893139 None:intergenic
TTAGGAGTGGAGTAGATGGA+GGG 0.598597 1_1:+7893140 None:intergenic
TTTGGTGTCAACAAAGGAGG+AGG 0.602071 1_1:+7893193 None:intergenic
GGTGCATTTGGTGTCAACAA+AGG 0.602133 1_1:+7893187 None:intergenic
GAAGGACTTGGTGGAGATGG+TGG 0.605778 1_1:+7892971 None:intergenic
GGTGGAAGACTTAGTGATGG+GGG 0.608316 1_1:+7893013 None:intergenic
GACGAAGGACTTGGTGGAGA+TGG 0.610720 1_1:+7892968 None:intergenic
GAATATGATGTTAATAGTGG+AGG 0.611804 1_1:+7893100 None:intergenic
GGTAGAGTCAACAATGGTGG+TGG 0.613265 1_1:+7892995 None:intergenic
GGACTTGGTGGAGATGGTGG+CGG 0.623426 1_1:+7892974 None:intergenic
GGAGAATTTGATGATGTGAG+TGG 0.631413 1_1:+7893067 None:intergenic
GCATTTGGTGTCAACAAAGG+AGG 0.639121 1_1:+7893190 None:intergenic
TCCTTCGTCACATTCTTCCG+GGG 0.641768 1_1:-7892954 Msa0005390:CDS
TGGTGGAAGACTTAGTGATG+GGG 0.649103 1_1:+7893012 None:intergenic
GAAGAAGGCGAAGATTTCAA+AGG 0.649156 1_1:+7892917 None:intergenic
GAAGGCGAAGATTTCAAAGG+TGG 0.657373 1_1:+7892920 None:intergenic
GGCGGTAGAGTCAACAATGG+TGG 0.660549 1_1:+7892992 None:intergenic
GGTGGTGGAAGACTTAGTGA+TGG 0.666350 1_1:+7893010 None:intergenic
GGTGTTATTAGTGGAAAAGG+GGG 0.675151 1_1:+7893166 None:intergenic
TCCCCGGAAGAATGTGACGA+AGG 0.679405 1_1:+7892953 None:intergenic
GATGATGTGAGTGGAAATGG+GGG 0.687997 1_1:+7893076 None:intergenic
GGTGGCGGTAGAGTCAACAA+TGG 0.730976 1_1:+7892989 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTGTATTCATGTTGT+TGG - chr1_1:7892810-7892829 Msa0005390:CDS 20.0%
!! TAATAATAGTAATAGGGATA+TGG + chr1_1:7893366-7893385 None:intergenic 20.0%
! TAGGATTCATAACAAAAAGT+TGG - chr1_1:7892773-7892792 Msa0005390:CDS 25.0%
!! CAACATGAATACAATTTTTG+TGG + chr1_1:7892809-7892828 None:intergenic 25.0%
! GAGGCATAATAATAGTAATA+GGG + chr1_1:7893372-7893391 None:intergenic 25.0%
! TGAGGCATAATAATAGTAAT+AGG + chr1_1:7893373-7893392 None:intergenic 25.0%
!! TAACAAAAAGTTGGTTTGCA+TGG - chr1_1:7892782-7892801 Msa0005390:CDS 30.0%
TTTGTTTGCAAAGAGAACAT+TGG - chr1_1:7892834-7892853 Msa0005390:CDS 30.0%
ATGCATAATAAACCACTTGA+AGG + chr1_1:7892886-7892905 None:intergenic 30.0%
GAATTTGATGAAGCAAGTTT+AGG + chr1_1:7892952-7892971 None:intergenic 30.0%
GAATATGATGTTAATAGTGG+AGG + chr1_1:7893108-7893127 None:intergenic 30.0%
GGTGAATATGATGTTAATAG+TGG + chr1_1:7893111-7893130 None:intergenic 30.0%
!! TATGATTTTAGAAGTGAAGG+AGG + chr1_1:7893168-7893187 None:intergenic 30.0%
!! GGATATGATTTTAGAAGTGA+AGG + chr1_1:7893171-7893190 None:intergenic 30.0%
TTCATCAGATATTCTTTGCT+TGG - chr1_1:7893448-7893467 Msa0005390:CDS 30.0%
ATGGGTCATAACAAGAATCT+AGG - chr1_1:7892754-7892773 Msa0005390:CDS 35.0%
AAAGAGAACATTGGAGATTG+AGG - chr1_1:7892843-7892862 Msa0005390:CDS 35.0%
TTTGATGAAGCAAGTTTAGG+AGG + chr1_1:7892949-7892968 None:intergenic 35.0%
! TATGATGTTAATAGTGGAGG+AGG + chr1_1:7893105-7893124 None:intergenic 35.0%
!! TTGATGATGTGAGTGGAAAT+GGG + chr1_1:7893134-7893153 None:intergenic 35.0%
!! TTTGATGATGTGAGTGGAAA+TGG + chr1_1:7893135-7893154 None:intergenic 35.0%
! TTTGAAATCTTCGCCTTCTT+CGG - chr1_1:7893290-7893309 Msa0005390:CDS 35.0%
CAAGGACAACCTAAAGTAAT+TGG - chr1_1:7893321-7893340 Msa0005390:CDS 35.0%
!! TAGGGATATGGATAGTTTTG+TGG + chr1_1:7893354-7893373 None:intergenic 35.0%
AGAAAAATGAGATGCACTTG+AGG + chr1_1:7893391-7893410 None:intergenic 35.0%
AAAGTTGAAGAGCCTTCAAG+TGG - chr1_1:7892871-7892890 Msa0005390:CDS 40.0%
! AGGTGAAGGATTTTGTGTTG+GGG + chr1_1:7892995-7893014 None:intergenic 40.0%
! GAGGTGAAGGATTTTGTGTT+GGG + chr1_1:7892996-7893015 None:intergenic 40.0%
!! GGTGTTATTAGTGGAAAAGG+GGG + chr1_1:7893042-7893061 None:intergenic 40.0%
!! GGGTGTTATTAGTGGAAAAG+GGG + chr1_1:7893043-7893062 None:intergenic 40.0%
!! GGGGTGTTATTAGTGGAAAA+GGG + chr1_1:7893044-7893063 None:intergenic 40.0%
!! TGGGGTGTTATTAGTGGAAA+AGG + chr1_1:7893045-7893064 None:intergenic 40.0%
! TGATGATGTGAGTGGAAATG+GGG + chr1_1:7893133-7893152 None:intergenic 40.0%
!! GGAGAATTTGATGATGTGAG+TGG + chr1_1:7893141-7893160 None:intergenic 40.0%
!! GATTTTAGAAGTGAAGGAGG+AGG + chr1_1:7893165-7893184 None:intergenic 40.0%
GAAGAAGGCGAAGATTTCAA+AGG + chr1_1:7893291-7893310 None:intergenic 40.0%
TTGAAATCTTCGCCTTCTTC+GGG - chr1_1:7893291-7893310 Msa0005390:CDS 40.0%
GGACAACCTAAAGTAATTGG+TGG - chr1_1:7893324-7893343 Msa0005390:CDS 40.0%
!!! GGATATGGATAGTTTTGTGG+TGG + chr1_1:7893351-7893370 None:intergenic 40.0%
! GGTGGTGGTGAACAAATTGT+AGG + chr1_1:7892928-7892947 None:intergenic 45.0%
GATGAAGCAAGTTTAGGAGG+TGG + chr1_1:7892946-7892965 None:intergenic 45.0%
! GGTGAAGGATTTTGTGTTGG+GGG + chr1_1:7892994-7893013 None:intergenic 45.0%
! GGAGGTGAAGGATTTTGTGT+TGG + chr1_1:7892997-7893016 None:intergenic 45.0%
!! TTTGGTGTCAACAAAGGAGG+AGG + chr1_1:7893015-7893034 None:intergenic 45.0%
!! GCATTTGGTGTCAACAAAGG+AGG + chr1_1:7893018-7893037 None:intergenic 45.0%
!! GGTGCATTTGGTGTCAACAA+AGG + chr1_1:7893021-7893040 None:intergenic 45.0%
TTAGGAGTGGAGTAGATGGA+GGG + chr1_1:7893068-7893087 None:intergenic 45.0%
TGTGTTAGGAGTGGAGTAGA+TGG + chr1_1:7893072-7893091 None:intergenic 45.0%
! GGAGGATTTTGTGTTAGGAG+TGG + chr1_1:7893081-7893100 None:intergenic 45.0%
! GAGGTGGAGGATTTTGTGTT+AGG + chr1_1:7893086-7893105 None:intergenic 45.0%
! GATGTTAATAGTGGAGGAGG+TGG + chr1_1:7893102-7893121 None:intergenic 45.0%
GATGATGTGAGTGGAAATGG+GGG + chr1_1:7893132-7893151 None:intergenic 45.0%
TTTAGAAGTGAAGGAGGAGG+AGG + chr1_1:7893162-7893181 None:intergenic 45.0%
TGGTGGAAGACTTAGTGATG+GGG + chr1_1:7893196-7893215 None:intergenic 45.0%
! GTGGTGGAAGACTTAGTGAT+GGG + chr1_1:7893197-7893216 None:intergenic 45.0%
GAAGAATGTGACGAAGGACT+TGG + chr1_1:7893249-7893268 None:intergenic 45.0%
AGTCCTTCGTCACATTCTTC+CGG - chr1_1:7893249-7893268 Msa0005390:CDS 45.0%
! GAAGATTTCAAAGGTGGTGG+AGG + chr1_1:7893282-7893301 None:intergenic 45.0%
GAAGGCGAAGATTTCAAAGG+TGG + chr1_1:7893288-7893307 None:intergenic 45.0%
GGTGGTCCACCAATTACTTT+AGG + chr1_1:7893333-7893352 None:intergenic 45.0%
GAAGCAAGTTTAGGAGGTGG+TGG + chr1_1:7892943-7892962 None:intergenic 50.0%
! TTTTGTGTTGGGGGTGAAGG+AGG + chr1_1:7892985-7893004 None:intergenic 50.0%
! GGATTTTGTGTTGGGGGTGA+AGG + chr1_1:7892988-7893007 None:intergenic 50.0%
GTCAACAAAGGAGGAGGTGA+AGG + chr1_1:7893009-7893028 None:intergenic 50.0%
! AGTGGAAAAGGGGGTGCATT+TGG + chr1_1:7893033-7893052 None:intergenic 50.0%
GTTAGGAGTGGAGTAGATGG+AGG + chr1_1:7893069-7893088 None:intergenic 50.0%
! GTTAATAGTGGAGGAGGTGG+AGG + chr1_1:7893099-7893118 None:intergenic 50.0%
GGTGGAAGACTTAGTGATGG+GGG + chr1_1:7893195-7893214 None:intergenic 50.0%
!! GGTGGTGGAAGACTTAGTGA+TGG + chr1_1:7893198-7893217 None:intergenic 50.0%
GGTAGAGTCAACAATGGTGG+TGG + chr1_1:7893213-7893232 None:intergenic 50.0%
GAATGTGACGAAGGACTTGG+TGG + chr1_1:7893246-7893265 None:intergenic 50.0%
GTCCTTCGTCACATTCTTCC+GGG - chr1_1:7893250-7893269 Msa0005390:CDS 50.0%
TCCTTCGTCACATTCTTCCG+GGG - chr1_1:7893251-7893270 Msa0005390:CDS 50.0%
GGCGAAGATTTCAAAGGTGG+TGG + chr1_1:7893285-7893304 None:intergenic 50.0%
!! GGAGGGTGGGGTGTTATTAG+TGG + chr1_1:7893051-7893070 None:intergenic 55.0%
AGTGGAGTAGATGGAGGGTG+GGG + chr1_1:7893063-7893082 None:intergenic 55.0%
GAGTGGAGTAGATGGAGGGT+GGG + chr1_1:7893064-7893083 None:intergenic 55.0%
! GGAAGACTTAGTGATGGGGG+AGG + chr1_1:7893192-7893211 None:intergenic 55.0%
GGCGGTAGAGTCAACAATGG+TGG + chr1_1:7893216-7893235 None:intergenic 55.0%
! GGTGGCGGTAGAGTCAACAA+TGG + chr1_1:7893219-7893238 None:intergenic 55.0%
!! GAAGGACTTGGTGGAGATGG+TGG + chr1_1:7893237-7893256 None:intergenic 55.0%
!! GACGAAGGACTTGGTGGAGA+TGG + chr1_1:7893240-7893259 None:intergenic 55.0%
TCCCCGGAAGAATGTGACGA+AGG + chr1_1:7893255-7893274 None:intergenic 55.0%
! TCTTCGCCTTCTTCGGGTTC+AGG - chr1_1:7893297-7893316 Msa0005390:CDS 55.0%
CCTTGACCTGAACCCGAAGA+AGG + chr1_1:7893306-7893325 None:intergenic 55.0%
! CCTTCTTCGGGTTCAGGTCA+AGG - chr1_1:7893303-7893322 Msa0005390:CDS 55.0%
GTGTTGGGGGTGAAGGAGGA+GGG + chr1_1:7892981-7893000 None:intergenic 60.0%
TGTGTTGGGGGTGAAGGAGG+AGG + chr1_1:7892982-7893001 None:intergenic 60.0%
GGAGTGGAGTAGATGGAGGG+TGG + chr1_1:7893065-7893084 None:intergenic 60.0%
!! GGACTTGGTGGAGATGGTGG+CGG + chr1_1:7893234-7893253 None:intergenic 60.0%
!! AGGTGGTGGAGGAGTTTCCC+CGG + chr1_1:7893271-7893290 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 7892754 7893473 7892754 ID=Msa0005390;Name=Msa0005390
chr1_1 mRNA 7892754 7893473 7892754 ID=Msa0005390-mRNA-1;Parent=Msa0005390;Name=Msa0005390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|239
chr1_1 exon 7892754 7893473 7892754 ID=Msa0005390-mRNA-1:exon:2823;Parent=Msa0005390-mRNA-1
chr1_1 CDS 7892754 7893473 7892754 ID=Msa0005390-mRNA-1:cds;Parent=Msa0005390-mRNA-1
Gene Sequence

>Msa0005390

ATGGGTCATAACAAGAATCTAGGATTCATAACAAAAAGTTGGTTTGCATGGACCACAAAAATTGTATTCATGTTGTTGGTTTTGTTTGCAAAGAGAACATTGGAGATTGAGGACAACAAAGTTGAAGAGCCTTCAAGTGGTTTATTATGCATTAGTGAATGTGTCACGTGTCCTACAATTTGTTCACCACCACCTCCTAAACTTGCTTCATCAAATTCAAATCACCCTCCTCCTTCACCCCCAACACAAAATCCTTCACCTCCTCCTTTGTTGACACCAAATGCACCCCCTTTTCCACTAATAACACCCCACCCTCCATCTACTCCACTCCTAACACAAAATCCTCCACCTCCTCCACTATTAACATCATATTCACCCCCATTTCCACTCACATCATCAAATTCTCCTCCTCCTCCTTCACTTCTAAAATCATATCCTCCCCCATCACTAAGTCTTCCACCACCATTGTTGACTCTACCGCCACCATCTCCACCAAGTCCTTCGTCACATTCTTCCGGGGAAACTCCTCCACCACCTTTGAAATCTTCGCCTTCTTCGGGTTCAGGTCAAGGACAACCTAAAGTAATTGGTGGACCACCACAAAACTATCCATATCCCTATTACTATTATTATGCCTCAAGTGCATCTCATTTTTCTAATCATGTTGTACTCTTCTTTATTGTACTTTTGTTCATTCATCAGATATTCTTTGCTTGGTAA

Protein sequence

>Msa0005390

MGHNKNLGFITKSWFAWTTKIVFMLLVLFAKRTLEIEDNKVEEPSSGLLCISECVTCPTICSPPPPKLASSNSNHPPPSPPTQNPSPPPLLTPNAPPFPLITPHPPSTPLLTQNPPPPPLLTSYSPPFPLTSSNSPPPPSLLKSYPPPSLSLPPPLLTLPPPSPPSPSSHSSGETPPPPLKSSPSSGSGQGQPKVIGGPPQNYPYPYYYYYASSASHFSNHVVLFFIVLLFIHQIFFAW*