Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723000 | XP_003613278.1 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723000 | sp|K4PW38|RSS3_ORYSJ | 36.444 | 225 | 105 | 7 | 14 | 227 | 31 | 228 | 8.58e-36 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723000 | G7K2R0 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000190 | Msa0723000 | 0.821482 | 3.944620e-53 | -8.615850e-47 |
| Msa0017510 | Msa0723000 | 0.825487 | 4.562908e-54 | -8.615850e-47 |
| Msa0029890 | Msa0723000 | 0.827693 | 1.358511e-54 | -8.615850e-47 |
| Msa0101810 | Msa0723000 | -0.816718 | 4.786863e-52 | -8.615850e-47 |
| Msa0110970 | Msa0723000 | 0.803356 | 3.625494e-49 | -8.615850e-47 |
| Msa0641360 | Msa0723000 | 0.804896 | 1.733446e-49 | -8.615850e-47 |
| Msa0643470 | Msa0723000 | 0.814219 | 1.721801e-51 | -8.615850e-47 |
| Msa0645050 | Msa0723000 | 0.803098 | 4.098996e-49 | -8.615850e-47 |
| Msa0682620 | Msa0723000 | 0.802728 | 4.887894e-49 | -8.615850e-47 |
| Msa0688770 | Msa0723000 | 0.813069 | 3.084962e-51 | -8.615850e-47 |
| Msa0719490 | Msa0723000 | -0.818424 | 1.974889e-52 | -8.615850e-47 |
| Msa0720070 | Msa0723000 | 0.805420 | 1.346536e-49 | -8.615850e-47 |
| Msa0722780 | Msa0723000 | 0.967776 | 9.562894e-128 | -8.615850e-47 |
| Msa0722850 | Msa0723000 | 0.951040 | 4.714834e-109 | -8.615850e-47 |
| Msa0723000 | Msa0723050 | 0.985647 | 3.192917e-164 | -8.615850e-47 |
| Msa0723000 | Msa0756630 | -0.817245 | 3.643800e-52 | -8.615850e-47 |
| Msa0723000 | Msa0802470 | -0.816634 | 4.998225e-52 | -8.615850e-47 |
| Msa0723000 | Msa0843220 | 0.916387 | 1.894281e-85 | -8.615850e-47 |
| Msa0723000 | Msa0880140 | 0.827277 | 1.709701e-54 | -8.615850e-47 |
| Msa0723000 | Msa0896970 | 0.806648 | 7.427758e-50 | -8.615850e-47 |
| Msa0723000 | Msa1008490 | 0.803034 | 4.226395e-49 | -8.615850e-47 |
| Msa0723000 | Msa1009000 | 0.817444 | 3.288167e-52 | -8.615850e-47 |
| Msa0723000 | Msa1055280 | 0.801333 | 9.460571e-49 | -8.615850e-47 |
| Msa0723000 | Msa1075220 | -0.816449 | 5.499102e-52 | -8.615850e-47 |
| Msa0723000 | Msa1077150 | 0.801770 | 7.697994e-49 | -8.615850e-47 |
| Msa0723000 | Msa1077180 | 0.833710 | 4.561140e-56 | -8.615850e-47 |
| Msa0723000 | Msa1083410 | 0.812090 | 5.050601e-51 | -8.615850e-47 |
| Msa0723000 | Msa1085300 | 0.801390 | 9.211039e-49 | -8.615850e-47 |
| Msa0723000 | Msa1098560 | 0.841866 | 3.664285e-58 | -8.615850e-47 |
| Msa0723000 | Msa1124680 | -0.808259 | 3.380996e-50 | -8.615850e-47 |
| Msa0723000 | Msa1162550 | -0.802138 | 6.465951e-49 | -8.615850e-47 |
| Msa0723000 | Msa1222970 | 0.827785 | 1.291135e-54 | -8.615850e-47 |
| Msa0723000 | Msa1228210 | 0.820612 | 6.256735e-53 | -8.615850e-47 |
| Msa0723000 | Msa1242220 | 0.813054 | 3.107545e-51 | -8.615850e-47 |
| Msa0723000 | Msa1242230 | 0.823881 | 1.090752e-53 | -8.615850e-47 |
| Msa0723000 | Msa1242240 | 0.806443 | 8.206630e-50 | -8.615850e-47 |
| Msa0723000 | Msa1251750 | 0.802575 | 5.256479e-49 | -8.615850e-47 |
| Msa0723000 | Msa1264020 | 0.801245 | 9.859597e-49 | -8.615850e-47 |
| Msa0723000 | Msa1271280 | 0.814619 | 1.405098e-51 | -8.615850e-47 |
| Msa0723000 | Msa1271300 | 0.800264 | 1.563642e-48 | -8.615850e-47 |
| Msa0723000 | Msa1278840 | 0.815567 | 8.651424e-52 | -8.615850e-47 |
| Msa0723000 | Msa1283840 | 0.801381 | 9.248552e-49 | -8.615850e-47 |
| Msa0723000 | Msa1283860 | 0.807576 | 4.724364e-50 | -8.615850e-47 |
| Msa0723000 | Msa1284260 | 0.815691 | 8.119327e-52 | -8.615850e-47 |
| Msa0723000 | Msa1293770 | 0.807912 | 4.008895e-50 | -8.615850e-47 |
| Msa0723000 | Msa1322860 | 0.827894 | 1.215575e-54 | -8.615850e-47 |
| Msa0723000 | Msa1322890 | 0.816842 | 4.489897e-52 | -8.615850e-47 |
| Msa0723000 | Msa1327050 | 0.815251 | 1.017194e-51 | -8.615850e-47 |
| Msa0723000 | Msa1367080 | 0.813286 | 2.764815e-51 | -8.615850e-47 |
| Msa0723000 | Msa1374550 | 0.862968 | 3.479482e-64 | -8.615850e-47 |
| Msa0125090 | Msa0723000 | 0.811578 | 6.528723e-51 | -8.615850e-47 |
| Msa0125100 | Msa0723000 | 0.821307 | 4.327664e-53 | -8.615850e-47 |
| Msa0140780 | Msa0723000 | 0.812166 | 4.859500e-51 | -8.615850e-47 |
| Msa0157290 | Msa0723000 | 0.825605 | 4.280328e-54 | -8.615850e-47 |
| Msa0169860 | Msa0723000 | 0.814660 | 1.376193e-51 | -8.615850e-47 |
| Msa0169870 | Msa0723000 | 0.801447 | 8.967340e-49 | -8.615850e-47 |
| Msa0194280 | Msa0723000 | 0.802808 | 4.705440e-49 | -8.615850e-47 |
| Msa0197230 | Msa0723000 | 0.810059 | 1.391266e-50 | -8.615850e-47 |
| Msa0202950 | Msa0723000 | 0.813620 | 2.334133e-51 | -8.615850e-47 |
| Msa0215320 | Msa0723000 | -0.800069 | 1.713558e-48 | -8.615850e-47 |
| Msa0221290 | Msa0723000 | 0.802236 | 6.175476e-49 | -8.615850e-47 |
| Msa0370030 | Msa0723000 | 0.802354 | 5.839446e-49 | -8.615850e-47 |
| Msa0385390 | Msa0723000 | 0.805362 | 1.384924e-49 | -8.615850e-47 |
| Msa0392420 | Msa0723000 | 0.838237 | 3.240597e-57 | -8.615850e-47 |
| Msa0392460 | Msa0723000 | 0.835702 | 1.439070e-56 | -8.615850e-47 |
| Msa0392490 | Msa0723000 | 0.837067 | 6.466305e-57 | -8.615850e-47 |
| Msa0465670 | Msa0723000 | -0.803392 | 3.564050e-49 | -8.615850e-47 |
| Msa0465910 | Msa0723000 | 0.802489 | 5.475752e-49 | -8.615850e-47 |
| Msa0465950 | Msa0723000 | 0.844791 | 6.074355e-59 | -8.615850e-47 |
| Msa0480980 | Msa0723000 | 0.810590 | 1.068990e-50 | -8.615850e-47 |
| Msa0243100 | Msa0723000 | 0.809699 | 1.663083e-50 | -8.615850e-47 |
| Msa0284420 | Msa0723000 | 0.811332 | 7.380812e-51 | -8.615850e-47 |
| Msa0344570 | Msa0723000 | 0.820496 | 6.653574e-53 | -8.615850e-47 |
| Msa0363310 | Msa0723000 | 0.803954 | 2.723571e-49 | -8.615850e-47 |
| Msa0487570 | Msa0723000 | 0.839981 | 1.144277e-57 | -8.615850e-47 |
| Msa0487630 | Msa0723000 | 0.842034 | 3.308329e-58 | -8.615850e-47 |
| Msa0503590 | Msa0723000 | 0.802790 | 4.747194e-49 | -8.615850e-47 |
| Msa0523460 | Msa0723000 | 0.802154 | 6.417159e-49 | -8.615850e-47 |
| Msa0535040 | Msa0723000 | 0.811084 | 8.355255e-51 | -8.615850e-47 |
| Msa0548280 | Msa0723000 | -0.814576 | 1.436353e-51 | -8.615850e-47 |
| Msa0581760 | Msa0723000 | 0.815940 | 7.147021e-52 | -8.615850e-47 |
| Msa0591790 | Msa0723000 | 0.805654 | 1.202605e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723000 | MtrunA17_Chr5g0413871 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Msa0723000 | MtrunA17_Chr1g0152981 | 51.185 | 211 | 80 | 5 | 13 | 217 | 11 | 204 | 6.14e-70 | 221 |
| Msa0723000 | MtrunA17_Chr2g0317011 | 38.537 | 205 | 99 | 6 | 14 | 218 | 20 | 197 | 5.12e-36 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723000 | AT1G60060.1 | 68.681 | 364 | 65 | 10 | 1 | 336 | 1 | 343 | 4.00e-160 | 454 |
| Msa0723000 | AT1G60060.2 | 64.263 | 319 | 65 | 10 | 46 | 336 | 6 | 303 | 1.12e-123 | 360 |
| Msa0723000 | AT5G53900.2 | 38.333 | 240 | 118 | 7 | 14 | 244 | 21 | 239 | 8.39e-39 | 142 |
| Msa0723000 | AT3G15240.2 | 37.073 | 205 | 107 | 4 | 14 | 218 | 34 | 216 | 9.58e-38 | 139 |
| Msa0723000 | AT5G53900.3 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0723000 | AT5G53900.1 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0723000 | AT2G31280.1 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 1.83e-14 | 75.1 |
| Msa0723000 | AT2G31280.3 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 2.08e-14 | 75.1 |
| Msa0723000 | AT2G27230.2 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
| Msa0723000 | AT2G27230.1 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
Find 102 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGCTCCTGCTGCTGTTTA+AGG | 0.126120 | 5_1:-20593703 | Msa0723000:three_prime_UTR |
| CCTAGCTCATCATCAGTTTA+TGG | 0.128324 | 5_1:-20595585 | Msa0723000:CDS |
| ATAAAATAATTAATAATTAT+TGG | 0.163550 | 5_1:+20593659 | None:intergenic |
| CAATCACCAGTGTTTATTTC+AGG | 0.239507 | 5_1:+20595609 | None:intergenic |
| TGCATATTTCAGTTTCTTCT+TGG | 0.255252 | 5_1:-20593876 | Msa0723000:three_prime_UTR |
| TCACATGAGATCTATAACTA+TGG | 0.263874 | 5_1:-20595504 | Msa0723000:CDS |
| CACACATTTGCATGGAATAA+TGG | 0.268865 | 5_1:+20593818 | None:intergenic |
| TTGGGTGGAGGGTAGTTTCT+AGG | 0.275012 | 5_1:+20596074 | None:intergenic |
| AACACAAACACAACATGTTT+TGG | 0.280192 | 5_1:-20594223 | Msa0723000:CDS |
| AGAGAAGGTGTTGTTCAATT+AGG | 0.283607 | 5_1:-20594933 | Msa0723000:CDS |
| AAATTAATTTCTTGATCATT+AGG | 0.297602 | 5_1:+20595305 | None:intergenic |
| CAACGAGCATTGGAATTTAC+AGG | 0.318102 | 5_1:-20594189 | Msa0723000:CDS |
| TATATGCATGCACAGGATAT+TGG | 0.324411 | 5_1:-20595673 | Msa0723000:intron |
| CATTTCCAAGATGATCTTGA+TGG | 0.328838 | 5_1:+20594046 | None:intergenic |
| AGGATATTGGTTTGGGAAGA+TGG | 0.329021 | 5_1:-20595660 | Msa0723000:CDS |
| TCTTGAAACCCTTTAGCTAT+AGG | 0.329720 | 5_1:+20596305 | None:intergenic |
| ATTGATGGAGTTACTTTCAT+TGG | 0.336300 | 5_1:+20594312 | None:intergenic |
| AATGGATCAAACAATAATAA+TGG | 0.339598 | 5_1:-20594027 | Msa0723000:CDS |
| CATCAAGATCATCTTGGAAA+TGG | 0.346285 | 5_1:-20594045 | Msa0723000:CDS |
| TCAGTTCTTGTCTGGTATAA+AGG | 0.362612 | 5_1:-20595097 | Msa0723000:intron |
| GCATGCACAGGATATTGGTT+TGG | 0.384339 | 5_1:-20595668 | Msa0723000:intron |
| GTGAGAGAGTTATTAATGTT+AGG | 0.386113 | 5_1:-20593936 | Msa0723000:three_prime_UTR |
| TCACCAACATCAAGTTGTTC+AGG | 0.392523 | 5_1:+20594108 | None:intergenic |
| GACATCTTGAAGAAAAGTTC+AGG | 0.396788 | 5_1:+20595525 | None:intergenic |
| CATGCACAGGATATTGGTTT+GGG | 0.400265 | 5_1:-20595667 | Msa0723000:intron |
| TCATCATCAGCATACCCTTA+TGG | 0.403770 | 5_1:-20594429 | Msa0723000:CDS |
| TAAAGATACCTATAGCTAAA+GGG | 0.404162 | 5_1:-20596313 | Msa0723000:five_prime_UTR |
| GTTGGTGTGTGCTTATATAA+AGG | 0.405928 | 5_1:-20596367 | Msa0723000:five_prime_UTR |
| GCTTCAAGACTACTCATTGA+TGG | 0.406362 | 5_1:+20594297 | None:intergenic |
| TTAAAGATACCTATAGCTAA+AGG | 0.409220 | 5_1:-20596314 | Msa0723000:five_prime_UTR |
| TGGGAAGCTCAGTTCTTGTC+TGG | 0.414012 | 5_1:-20595105 | Msa0723000:CDS |
| CTAGCTCATCATCAGTTTAT+GGG | 0.418675 | 5_1:-20595584 | Msa0723000:CDS |
| CTCTCTCACTACAATATGTT+AGG | 0.427654 | 5_1:+20593950 | None:intergenic |
| TAGGTATCTTTAATAATAGT+AGG | 0.427738 | 5_1:+20596324 | None:intergenic |
| CCAATCTACTACATTGTACT+TGG | 0.431351 | 5_1:-20593740 | Msa0723000:three_prime_UTR |
| TAAATGTGATATTTCCTTGT+TGG | 0.431856 | 5_1:-20596385 | Msa0723000:five_prime_UTR |
| CATTTCCATCAAGATCATCT+TGG | 0.434289 | 5_1:-20594051 | Msa0723000:CDS |
| TTTACAGCACCCTAGGACTT+GGG | 0.440440 | 5_1:-20595124 | Msa0723000:intron |
| CAAGAGGAAACAGAAGGAAT+TGG | 0.444449 | 5_1:-20595815 | Msa0723000:intron |
| TGTTCTGATGGACCACCATA+AGG | 0.450763 | 5_1:+20594414 | None:intergenic |
| GGTAGTGTATTGTAGTATGA+AGG | 0.452877 | 5_1:-20593855 | Msa0723000:three_prime_UTR |
| CTATGATCTTCTTACTTGGG+TGG | 0.478789 | 5_1:+20596059 | None:intergenic |
| TGATCTTCTTACTTGGGTGG+AGG | 0.479359 | 5_1:+20596062 | None:intergenic |
| GAACAATGGCACAACTTTCA+AGG | 0.484632 | 5_1:-20594396 | Msa0723000:CDS |
| GTTGGATAATGAAGAAGAAG+AGG | 0.487343 | 5_1:-20594139 | Msa0723000:CDS |
| CCAAGTACAATGTAGTAGAT+TGG | 0.489579 | 5_1:+20593740 | None:intergenic |
| AACTGAGCTTCCCAAGTCCT+AGG | 0.490005 | 5_1:+20595114 | None:intergenic |
| AGCTACATTGAAAGCATACC+AGG | 0.501005 | 5_1:-20594471 | Msa0723000:CDS |
| GTTCTGATGGACCACCATAA+GGG | 0.509808 | 5_1:+20594415 | None:intergenic |
| GAGATCTATAACTATGGAGA+AGG | 0.511952 | 5_1:-20595498 | Msa0723000:CDS |
| TAATATAGGTGGGAAGGACA+AGG | 0.515798 | 5_1:-20595852 | Msa0723000:intron |
| TAAGGGTATGCTGATGATGA+TGG | 0.520173 | 5_1:+20594432 | None:intergenic |
| GATCTTCTTACTTGGGTGGA+GGG | 0.521140 | 5_1:+20596063 | None:intergenic |
| TGTGTATAATATAGGTGGGA+AGG | 0.521340 | 5_1:-20595858 | Msa0723000:intron |
| TTGCATCCTTAAACAGCAGC+AGG | 0.525427 | 5_1:+20593697 | None:intergenic |
| AACACAACAACAACGAGCAT+TGG | 0.528793 | 5_1:-20594199 | Msa0723000:CDS |
| AAGTTGTGCCATTGTTCTGA+TGG | 0.534050 | 5_1:+20594402 | None:intergenic |
| ACAATCTTCACACATTTGCA+TGG | 0.536085 | 5_1:+20593810 | None:intergenic |
| GGAAGAACATCTTACTCCAT+TGG | 0.536644 | 5_1:-20596186 | Msa0723000:CDS |
| AAAAGTCGCGAAAGAAGAGT+TGG | 0.538902 | 5_1:-20594157 | Msa0723000:CDS |
| TCAGGTTGAAGACCTTGGTA+AGG | 0.540371 | 5_1:+20595543 | None:intergenic |
| CCATAAACTGATGATGAGCT+AGG | 0.545039 | 5_1:+20595585 | None:intergenic |
| GGGTGTGGAAGTAGCACTCC+TGG | 0.547961 | 5_1:+20594453 | None:intergenic |
| AAAGTTCAGGTTGAAGACCT+TGG | 0.548638 | 5_1:+20595538 | None:intergenic |
| GTGCTTATATAAAGGAGGAT+AGG | 0.553115 | 5_1:-20596359 | Msa0723000:five_prime_UTR |
| AAATTAATTTCTTGTCAGCA+TGG | 0.554884 | 5_1:-20595292 | Msa0723000:CDS |
| ACCTTCTCTAACAGCAATGA+GGG | 0.566133 | 5_1:+20594947 | None:intergenic |
| ACAGCAGCAGGAGCTAGCAT+TGG | 0.566143 | 5_1:+20593709 | None:intergenic |
| CACCCACCTAAATAAAATGA+GGG | 0.571153 | 5_1:-20596418 | None:intergenic |
| AGAAGATGAGTAACAGCCAA+TGG | 0.572438 | 5_1:+20596170 | None:intergenic |
| GTAGTGTATTGTAGTATGAA+GGG | 0.572719 | 5_1:-20593854 | Msa0723000:three_prime_UTR |
| AGGTATCTTTAATAATAGTA+GGG | 0.573912 | 5_1:+20596325 | None:intergenic |
| CTGCAGATCATAGTCACAAG+TGG | 0.577564 | 5_1:-20595340 | Msa0723000:CDS |
| CATCCATGAAAATTCTCAGT+GGG | 0.580339 | 5_1:-20596123 | Msa0723000:CDS |
| GAGAGTAGTAGTTCCATACC+AGG | 0.580651 | 5_1:-20594087 | Msa0723000:CDS |
| TATGCTGATGATGATGGGTG+TGG | 0.582762 | 5_1:+20594438 | None:intergenic |
| AATATAGGTGGGAAGGACAA+GGG | 0.583406 | 5_1:-20595851 | Msa0723000:intron |
| ACTGAGCTTCCCAAGTCCTA+GGG | 0.584163 | 5_1:+20595115 | None:intergenic |
| CACCTTCTCTAACAGCAATG+AGG | 0.591099 | 5_1:+20594946 | None:intergenic |
| ATGTTGATGATACCCTGGTA+TGG | 0.591188 | 5_1:+20594074 | None:intergenic |
| GCCCTCATTGCTGTTAGAGA+AGG | 0.593948 | 5_1:-20594948 | Msa0723000:CDS |
| ATATAGGTGGGAAGGACAAG+GGG | 0.594932 | 5_1:-20595850 | Msa0723000:intron |
| AGCATTGCACCACAGCATCA+AGG | 0.600171 | 5_1:-20594372 | Msa0723000:CDS |
| GGGGCATATGATAGATCAAG+AGG | 0.613030 | 5_1:-20595831 | Msa0723000:CDS |
| TGTTGTGGTGGAACAACTGA+AGG | 0.615956 | 5_1:+20594258 | None:intergenic |
| AGATCTATAACTATGGAGAA+GGG | 0.626888 | 5_1:-20595497 | Msa0723000:intron |
| TCAATTAGGAGCTGTTCACA+AGG | 0.628502 | 5_1:-20594919 | Msa0723000:intron |
| ATAGATCAAGAGGAAACAGA+AGG | 0.628702 | 5_1:-20595821 | Msa0723000:CDS |
| AAGGGTATGCTGATGATGAT+GGG | 0.632300 | 5_1:+20594433 | None:intergenic |
| AATTGATCACCTTGATGCTG+TGG | 0.645054 | 5_1:+20594363 | None:intergenic |
| AGACCTGAACAACTTGATGT+TGG | 0.656658 | 5_1:-20594111 | Msa0723000:CDS |
| TATATAAGCACACACCAACA+AGG | 0.657069 | 5_1:+20596371 | None:intergenic |
| GAATTAATTCAACCTTACCA+AGG | 0.657107 | 5_1:-20595555 | Msa0723000:CDS |
| ATGGTGGTCCATCAGAACAA+TGG | 0.666115 | 5_1:-20594410 | Msa0723000:CDS |
| GATTGAAGACTTGAGCTATG+TGG | 0.668398 | 5_1:-20594514 | Msa0723000:CDS |
| GGTGTGTGCTTATATAAAGG+AGG | 0.669304 | 5_1:-20596364 | Msa0723000:five_prime_UTR |
| TCATCAGCATACCCTTATGG+TGG | 0.673613 | 5_1:-20594426 | Msa0723000:CDS |
| AAATGATGTTGATGATACCC+TGG | 0.680740 | 5_1:+20594069 | None:intergenic |
| ACCCACCTAAATAAAATGAG+GGG | 0.695884 | 5_1:-20596417 | None:intergenic |
| GCAGCACCTGAAATAAACAC+TGG | 0.697600 | 5_1:-20595615 | Msa0723000:CDS |
| GCATCCATGAAAATTCTCAG+TGG | 0.703195 | 5_1:-20596124 | Msa0723000:CDS |
| AGAGTAGTAGTTCCATACCA+GGG | 0.724689 | 5_1:-20594086 | Msa0723000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAATTAATAATTAT+TGG | + | chr5_1:20596363-20596382 | None:intergenic | 0.0% |
| !!! | TATTAGTTAATTAGATTTTA+TGG | - | chr5_1:20594634-20594653 | Msa0723000:intron | 10.0% |
| !! | AATTGAATTAAATTAAAGAA+AGG | + | chr5_1:20595026-20595045 | None:intergenic | 10.0% |
| !! | TGCATATATATATATATATG+AGG | - | chr5_1:20594064-20594083 | Msa0723000:CDS | 15.0% |
| !!! | ATGTTTTTTGTGTATAATAT+AGG | - | chr5_1:20594153-20594172 | Msa0723000:CDS | 15.0% |
| !! | ACTTGATTATAAAAAAACAA+TGG | - | chr5_1:20594255-20594274 | Msa0723000:CDS | 15.0% |
| !! | AAATTAATTTCTTGATCATT+AGG | + | chr5_1:20594717-20594736 | None:intergenic | 15.0% |
| !! | AGGTATCTTTAATAATAGTA+GGG | + | chr5_1:20593697-20593716 | None:intergenic | 20.0% |
| !! | TAGGTATCTTTAATAATAGT+AGG | + | chr5_1:20593698-20593717 | None:intergenic | 20.0% |
| !!! | TTTTTTGTGTATAATATAGG+TGG | - | chr5_1:20594156-20594175 | Msa0723000:CDS | 20.0% |
| !!! | TTTTTGTGTATAATATAGGT+GGG | - | chr5_1:20594157-20594176 | Msa0723000:CDS | 20.0% |
| !!! | AGTTTTAACTTTAAGCAAAT+AGG | - | chr5_1:20594609-20594628 | Msa0723000:intron | 20.0% |
| !!! | AAATAAAAGTATGTGAATGA+GGG | + | chr5_1:20594984-20595003 | None:intergenic | 20.0% |
| !!! | TAAATAAAAGTATGTGAATG+AGG | + | chr5_1:20594985-20595004 | None:intergenic | 20.0% |
| !! | CTGGTAATAATTTAAGAAAA+TGG | + | chr5_1:20595284-20595303 | None:intergenic | 20.0% |
| !! | CATAAAAAATCATGATATCA+TGG | - | chr5_1:20595368-20595387 | Msa0723000:intron | 20.0% |
| !! | AATGGATCAAACAATAATAA+TGG | - | chr5_1:20595992-20596011 | Msa0723000:intron | 20.0% |
| ! | TAAATGTGATATTTCCTTGT+TGG | - | chr5_1:20593634-20593653 | Msa0723000:three_prime_UTR | 25.0% |
| ! | TTAAAGATACCTATAGCTAA+AGG | - | chr5_1:20593705-20593724 | Msa0723000:three_prime_UTR | 25.0% |
| ! | TAAAGATACCTATAGCTAAA+GGG | - | chr5_1:20593706-20593725 | Msa0723000:three_prime_UTR | 25.0% |
| !! | TTAATTAGATTTTATGGAGC+AGG | - | chr5_1:20594640-20594659 | Msa0723000:intron | 25.0% |
| ! | AAATTAATTTCTTGTCAGCA+TGG | - | chr5_1:20594727-20594746 | Msa0723000:intron | 25.0% |
| ! | GAAATCATTCTATCTATTTC+AGG | + | chr5_1:20594874-20594893 | None:intergenic | 25.0% |
| ! | TTTGATTGAGTGAGTATAAT+TGG | + | chr5_1:20595461-20595480 | None:intergenic | 25.0% |
| !! | TCAATCAAATTTTGTCTCTA+TGG | - | chr5_1:20595472-20595491 | Msa0723000:intron | 25.0% |
| !! | TTTTGTCTCTATGGAATTTA+AGG | - | chr5_1:20595481-20595500 | Msa0723000:intron | 25.0% |
| !!! | GTTTGATTTTGAATTTGTTG+TGG | + | chr5_1:20595779-20595798 | None:intergenic | 25.0% |
| TTGCTATGATCTTCTTACTT+GGG | + | chr5_1:20593966-20593985 | None:intergenic | 30.0% | |
| ! | TTTGCTATGATCTTCTTACT+TGG | + | chr5_1:20593967-20593986 | None:intergenic | 30.0% |
| GAATTAATTCAACCTTACCA+AGG | - | chr5_1:20594464-20594483 | Msa0723000:CDS | 30.0% | |
| TCACATGAGATCTATAACTA+TGG | - | chr5_1:20594515-20594534 | Msa0723000:CDS | 30.0% | |
| AGATCTATAACTATGGAGAA+GGG | - | chr5_1:20594522-20594541 | Msa0723000:intron | 30.0% | |
| ! | ACCATGTTTGAGATAACAAA+GGG | + | chr5_1:20595137-20595156 | None:intergenic | 30.0% |
| ! | AACCATGTTTGAGATAACAA+AGG | + | chr5_1:20595138-20595157 | None:intergenic | 30.0% |
| ! | TTTCAGATTTAAGACGTGTT+TGG | - | chr5_1:20595193-20595212 | Msa0723000:intron | 30.0% |
| TGTTAGACACAAACATAAGT+CGG | + | chr5_1:20595222-20595241 | None:intergenic | 30.0% | |
| !!! | GTGCAATGTTTTAAAGACAA+AGG | + | chr5_1:20595412-20595431 | None:intergenic | 30.0% |
| ! | ATTGATGGAGTTACTTTCAT+TGG | + | chr5_1:20595710-20595729 | None:intergenic | 30.0% |
| !!! | TGATTTTGAATTTGTTGTGG+TGG | + | chr5_1:20595776-20595795 | None:intergenic | 30.0% |
| ! | AACACAAACACAACATGTTT+TGG | - | chr5_1:20595796-20595815 | Msa0723000:intron | 30.0% |
| GTGAGAGAGTTATTAATGTT+AGG | - | chr5_1:20596083-20596102 | Msa0723000:CDS | 30.0% | |
| TGCATATTTCAGTTTCTTCT+TGG | - | chr5_1:20596143-20596162 | Msa0723000:CDS | 30.0% | |
| GTAGTGTATTGTAGTATGAA+GGG | - | chr5_1:20596165-20596184 | Msa0723000:CDS | 30.0% | |
| TATATAAGCACACACCAACA+AGG | + | chr5_1:20593651-20593670 | None:intergenic | 35.0% | |
| GTTGGTGTGTGCTTATATAA+AGG | - | chr5_1:20593652-20593671 | Msa0723000:three_prime_UTR | 35.0% | |
| GTGCTTATATAAAGGAGGAT+AGG | - | chr5_1:20593660-20593679 | Msa0723000:three_prime_UTR | 35.0% | |
| TCTTGAAACCCTTTAGCTAT+AGG | + | chr5_1:20593717-20593736 | None:intergenic | 35.0% | |
| CATCCATGAAAATTCTCAGT+GGG | - | chr5_1:20593896-20593915 | Msa0723000:three_prime_UTR | 35.0% | |
| ! | AAACCCACTGAGAATTTTCA+TGG | + | chr5_1:20593902-20593921 | None:intergenic | 35.0% |
| TGTGTATAATATAGGTGGGA+AGG | - | chr5_1:20594161-20594180 | Msa0723000:CDS | 35.0% | |
| ATAGATCAAGAGGAAACAGA+AGG | - | chr5_1:20594198-20594217 | Msa0723000:CDS | 35.0% | |
| TCTATGTTATATGCATGCAC+AGG | - | chr5_1:20594339-20594358 | Msa0723000:CDS | 35.0% | |
| TATATGCATGCACAGGATAT+TGG | - | chr5_1:20594346-20594365 | Msa0723000:CDS | 35.0% | |
| CAATCACCAGTGTTTATTTC+AGG | + | chr5_1:20594413-20594432 | None:intergenic | 35.0% | |
| CTAGCTCATCATCAGTTTAT+GGG | - | chr5_1:20594435-20594454 | Msa0723000:CDS | 35.0% | |
| GACATCTTGAAGAAAAGTTC+AGG | + | chr5_1:20594497-20594516 | None:intergenic | 35.0% | |
| GAGATCTATAACTATGGAGA+AGG | - | chr5_1:20594521-20594540 | Msa0723000:intron | 35.0% | |
| !!! | ATTTTATGGAGCAGGTTGAT+TGG | - | chr5_1:20594648-20594667 | Msa0723000:intron | 35.0% |
| TCAGTTCTTGTCTGGTATAA+AGG | - | chr5_1:20594922-20594941 | Msa0723000:CDS | 35.0% | |
| !! | AGAGAAGGTGTTGTTCAATT+AGG | - | chr5_1:20595086-20595105 | Msa0723000:intron | 35.0% |
| ! | TCCCTTTGTTATCTCAAACA+TGG | - | chr5_1:20595133-20595152 | Msa0723000:intron | 35.0% |
| ATGAAACACAACACTGATAC+TGG | + | chr5_1:20595303-20595322 | None:intergenic | 35.0% | |
| GTATCAGTGTTGTGTTTCAT+AGG | - | chr5_1:20595303-20595322 | Msa0723000:CDS | 35.0% | |
| GTTGGATAATGAAGAAGAAG+AGG | - | chr5_1:20595880-20595899 | Msa0723000:intron | 35.0% | |
| !! | AAATGATGTTGATGATACCC+TGG | + | chr5_1:20595953-20595972 | None:intergenic | 35.0% |
| CATTTCCATCAAGATCATCT+TGG | - | chr5_1:20595968-20595987 | Msa0723000:intron | 35.0% | |
| CATTTCCAAGATGATCTTGA+TGG | + | chr5_1:20595976-20595995 | None:intergenic | 35.0% | |
| CATCAAGATCATCTTGGAAA+TGG | - | chr5_1:20595974-20595993 | Msa0723000:intron | 35.0% | |
| CTCTCTCACTACAATATGTT+AGG | + | chr5_1:20596072-20596091 | None:intergenic | 35.0% | |
| GGTAGTGTATTGTAGTATGA+AGG | - | chr5_1:20596164-20596183 | Msa0723000:CDS | 35.0% | |
| CACACATTTGCATGGAATAA+TGG | + | chr5_1:20596204-20596223 | None:intergenic | 35.0% | |
| ACAATCTTCACACATTTGCA+TGG | + | chr5_1:20596212-20596231 | None:intergenic | 35.0% | |
| CCAAGTACAATGTAGTAGAT+TGG | + | chr5_1:20596282-20596301 | None:intergenic | 35.0% | |
| CCAATCTACTACATTGTACT+TGG | - | chr5_1:20596279-20596298 | Msa0723000:five_prime_UTR | 35.0% | |
| GGTGTGTGCTTATATAAAGG+AGG | - | chr5_1:20593655-20593674 | Msa0723000:three_prime_UTR | 40.0% | |
| GGAAGAACATCTTACTCCAT+TGG | - | chr5_1:20593833-20593852 | Msa0723000:three_prime_UTR | 40.0% | |
| AGAAGATGAGTAACAGCCAA+TGG | + | chr5_1:20593852-20593871 | None:intergenic | 40.0% | |
| GCATCCATGAAAATTCTCAG+TGG | - | chr5_1:20593895-20593914 | Msa0723000:three_prime_UTR | 40.0% | |
| !!! | AGTGGGTTTATGCTGTTTTC+TGG | - | chr5_1:20593913-20593932 | Msa0723000:three_prime_UTR | 40.0% |
| CTATGATCTTCTTACTTGGG+TGG | + | chr5_1:20593963-20593982 | None:intergenic | 40.0% | |
| TAATATAGGTGGGAAGGACA+AGG | - | chr5_1:20594167-20594186 | Msa0723000:CDS | 40.0% | |
| AATATAGGTGGGAAGGACAA+GGG | - | chr5_1:20594168-20594187 | Msa0723000:CDS | 40.0% | |
| CAAGAGGAAACAGAAGGAAT+TGG | - | chr5_1:20594204-20594223 | Msa0723000:CDS | 40.0% | |
| !! | CATGCACAGGATATTGGTTT+GGG | - | chr5_1:20594352-20594371 | Msa0723000:CDS | 40.0% |
| !! | AGGATATTGGTTTGGGAAGA+TGG | - | chr5_1:20594359-20594378 | Msa0723000:CDS | 40.0% |
| ! | CCATAAACTGATGATGAGCT+AGG | + | chr5_1:20594437-20594456 | None:intergenic | 40.0% |
| CCTAGCTCATCATCAGTTTA+TGG | - | chr5_1:20594434-20594453 | Msa0723000:CDS | 40.0% | |
| !! | AAAGTTCAGGTTGAAGACCT+TGG | + | chr5_1:20594484-20594503 | None:intergenic | 40.0% |
| ! | GATTTCTTTTACAGCACCCT+AGG | - | chr5_1:20594888-20594907 | Msa0723000:intron | 40.0% |
| ACCTTCTCTAACAGCAATGA+GGG | + | chr5_1:20595075-20595094 | None:intergenic | 40.0% | |
| TCAATTAGGAGCTGTTCACA+AGG | - | chr5_1:20595100-20595119 | Msa0723000:CDS | 40.0% | |
| GATTGAAGACTTGAGCTATG+TGG | - | chr5_1:20595505-20595524 | Msa0723000:CDS | 40.0% | |
| ! | AGCTACATTGAAAGCATACC+AGG | - | chr5_1:20595548-20595567 | Msa0723000:CDS | 40.0% |
| ! | AAGGGTATGCTGATGATGAT+GGG | + | chr5_1:20595589-20595608 | None:intergenic | 40.0% |
| ! | TAAGGGTATGCTGATGATGA+TGG | + | chr5_1:20595590-20595609 | None:intergenic | 40.0% |
| TCATCATCAGCATACCCTTA+TGG | - | chr5_1:20595590-20595609 | Msa0723000:CDS | 40.0% | |
| ! | AAGTTGTGCCATTGTTCTGA+TGG | + | chr5_1:20595620-20595639 | None:intergenic | 40.0% |
| GAACAATGGCACAACTTTCA+AGG | - | chr5_1:20595623-20595642 | Msa0723000:CDS | 40.0% | |
| AATTGATCACCTTGATGCTG+TGG | + | chr5_1:20595659-20595678 | None:intergenic | 40.0% | |
| GCTTCAAGACTACTCATTGA+TGG | + | chr5_1:20595725-20595744 | None:intergenic | 40.0% | |
| AACACAACAACAACGAGCAT+TGG | - | chr5_1:20595820-20595839 | Msa0723000:CDS | 40.0% | |
| !! | CAACGAGCATTGGAATTTAC+AGG | - | chr5_1:20595830-20595849 | Msa0723000:CDS | 40.0% |
| AAAAGTCGCGAAAGAAGAGT+TGG | - | chr5_1:20595862-20595881 | Msa0723000:intron | 40.0% | |
| AGACCTGAACAACTTGATGT+TGG | - | chr5_1:20595908-20595927 | Msa0723000:intron | 40.0% | |
| ! | TCACCAACATCAAGTTGTTC+AGG | + | chr5_1:20595914-20595933 | None:intergenic | 40.0% |
| AGAGTAGTAGTTCCATACCA+GGG | - | chr5_1:20595933-20595952 | Msa0723000:intron | 40.0% | |
| !! | ATGTTGATGATACCCTGGTA+TGG | + | chr5_1:20595948-20595967 | None:intergenic | 40.0% |
| !!! | GGGTTTATGCTGTTTTCTGG+AGG | - | chr5_1:20593916-20593935 | Msa0723000:three_prime_UTR | 45.0% |
| GATCTTCTTACTTGGGTGGA+GGG | + | chr5_1:20593959-20593978 | None:intergenic | 45.0% | |
| TGATCTTCTTACTTGGGTGG+AGG | + | chr5_1:20593960-20593979 | None:intergenic | 45.0% | |
| ATATAGGTGGGAAGGACAAG+GGG | - | chr5_1:20594169-20594188 | Msa0723000:CDS | 45.0% | |
| GGGGCATATGATAGATCAAG+AGG | - | chr5_1:20594188-20594207 | Msa0723000:CDS | 45.0% | |
| ! | GCATGCACAGGATATTGGTT+TGG | - | chr5_1:20594351-20594370 | Msa0723000:CDS | 45.0% |
| GCAGCACCTGAAATAAACAC+TGG | - | chr5_1:20594404-20594423 | Msa0723000:CDS | 45.0% | |
| !! | TCAGGTTGAAGACCTTGGTA+AGG | + | chr5_1:20594479-20594498 | None:intergenic | 45.0% |
| CTGCAGATCATAGTCACAAG+TGG | - | chr5_1:20594679-20594698 | Msa0723000:intron | 45.0% | |
| ! | TTTTACAGCACCCTAGGACT+TGG | - | chr5_1:20594894-20594913 | Msa0723000:intron | 45.0% |
| TTTACAGCACCCTAGGACTT+GGG | - | chr5_1:20594895-20594914 | Msa0723000:intron | 45.0% | |
| CACCTTCTCTAACAGCAATG+AGG | + | chr5_1:20595076-20595095 | None:intergenic | 45.0% | |
| AACCGCAACCATGTTGCAAT+CGG | - | chr5_1:20595340-20595359 | Msa0723000:CDS | 45.0% | |
| ATGTCTCTCCGATTGCAACA+TGG | + | chr5_1:20595351-20595370 | None:intergenic | 45.0% | |
| TCATGGATCAGTCGCATTCT+CGG | - | chr5_1:20595385-20595404 | Msa0723000:intron | 45.0% | |
| !! | TATGCTGATGATGATGGGTG+TGG | + | chr5_1:20595584-20595603 | None:intergenic | 45.0% |
| TCATCAGCATACCCTTATGG+TGG | - | chr5_1:20595593-20595612 | Msa0723000:CDS | 45.0% | |
| ! | GTTCTGATGGACCACCATAA+GGG | + | chr5_1:20595607-20595626 | None:intergenic | 45.0% |
| ! | TGTTCTGATGGACCACCATA+AGG | + | chr5_1:20595608-20595627 | None:intergenic | 45.0% |
| ATGGTGGTCCATCAGAACAA+TGG | - | chr5_1:20595609-20595628 | Msa0723000:CDS | 45.0% | |
| ! | TGTTGTGGTGGAACAACTGA+AGG | + | chr5_1:20595764-20595783 | None:intergenic | 45.0% |
| GAGAGTAGTAGTTCCATACC+AGG | - | chr5_1:20595932-20595951 | Msa0723000:intron | 45.0% | |
| TTGCATCCTTAAACAGCAGC+AGG | + | chr5_1:20596325-20596344 | None:intergenic | 45.0% | |
| !!! | TAAGAATTTTATTTTATATA+TGG | - | chr5_1:20593812-20593831 | Msa0723000:three_prime_UTR | 5.0% |
| TTGGGTGGAGGGTAGTTTCT+AGG | + | chr5_1:20593948-20593967 | None:intergenic | 50.0% | |
| ACTGAGCTTCCCAAGTCCTA+GGG | + | chr5_1:20594907-20594926 | None:intergenic | 50.0% | |
| AACTGAGCTTCCCAAGTCCT+AGG | + | chr5_1:20594908-20594927 | None:intergenic | 50.0% | |
| TGGGAAGCTCAGTTCTTGTC+TGG | - | chr5_1:20594914-20594933 | Msa0723000:intron | 50.0% | |
| ! | GCCCTCATTGCTGTTAGAGA+AGG | - | chr5_1:20595071-20595090 | Msa0723000:intron | 50.0% |
| CTCCGATTGCAACATGGTTG+CGG | + | chr5_1:20595345-20595364 | None:intergenic | 50.0% | |
| AGCATTGCACCACAGCATCA+AGG | - | chr5_1:20595647-20595666 | Msa0723000:CDS | 50.0% | |
| ! | CTAGCTCCTGCTGCTGTTTA+AGG | - | chr5_1:20596316-20596335 | Msa0723000:five_prime_UTR | 50.0% |
| ACAGCAGCAGGAGCTAGCAT+TGG | + | chr5_1:20596313-20596332 | None:intergenic | 55.0% | |
| GGGTGTGGAAGTAGCACTCC+TGG | + | chr5_1:20595569-20595588 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 20593616 | 20596425 | 20593616 | ID=Msa0723000;Name=Msa0723000 |
| chr5_1 | mRNA | 20593616 | 20596425 | 20593616 | ID=Msa0723000-mRNA-1;Parent=Msa0723000;Name=Msa0723000-mRNA-1;_AED=0.16;_eAED=0.16;_QI=215|1|1|1|1|1|7|404|364 |
| chr5_1 | exon | 20593616 | 20594538 | 20593616 | ID=Msa0723000-mRNA-1:exon:7873;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20594920 | 20594976 | 20594920 | ID=Msa0723000-mRNA-1:exon:7872;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20595098 | 20595139 | 20595098 | ID=Msa0723000-mRNA-1:exon:7871;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20595274 | 20595379 | 20595274 | ID=Msa0723000-mRNA-1:exon:7870;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20595498 | 20595680 | 20595498 | ID=Msa0723000-mRNA-1:exon:7869;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20595816 | 20595866 | 20595816 | ID=Msa0723000-mRNA-1:exon:7868;Parent=Msa0723000-mRNA-1 |
| chr5_1 | exon | 20596074 | 20596425 | 20596074 | ID=Msa0723000-mRNA-1:exon:7867;Parent=Msa0723000-mRNA-1 |
| chr5_1 | five_prime_UTR | 20596211 | 20596425 | 20596211 | ID=Msa0723000-mRNA-1:five_prime_utr;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20596074 | 20596210 | 20596074 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20595816 | 20595866 | 20595816 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20595498 | 20595680 | 20595498 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20595274 | 20595379 | 20595274 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20595098 | 20595139 | 20595098 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20594920 | 20594976 | 20594920 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | CDS | 20594020 | 20594538 | 20594020 | ID=Msa0723000-mRNA-1:cds;Parent=Msa0723000-mRNA-1 |
| chr5_1 | three_prime_UTR | 20593616 | 20594019 | 20593616 | ID=Msa0723000-mRNA-1:three_prime_utr;Parent=Msa0723000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
