AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1346280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1346280 MtrunA17_Chr2g0307941 96.774 217 7 0 1 217 737 953 2.28e-141 418
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1346280 AT3G42660.1 42.035 226 107 6 1 216 742 953 7.86e-38 139

Find 35 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCTGAAACTTCCAAATT+TGG 0.195450 tig0008586:-90648 Msa1346280:CDS
TGATGACGATGCTTTCAATT+TGG 0.265584 tig0008586:-90894 Msa1346280:CDS
ATACTTCCGTTAACATTTGC+AGG 0.278775 tig0008586:+90299 None:intergenic
AATAGATGCTTTCGATGTTT+CGG 0.338697 tig0008586:+90406 None:intergenic
TGACAAATCGGAGCCTAGTT+TGG 0.385690 tig0008586:-90171 Msa1346280:CDS
AATAAACCTGCAAATGTTAA+CGG 0.395713 tig0008586:-90305 Msa1346280:CDS
CTTGTTGGTGTCACCAAACT+AGG 0.423697 tig0008586:+90158 None:intergenic
AGAAATGGATAATGCTGGTT+TGG 0.423905 tig0008586:-90930 Msa1346280:CDS
GGTAGAGTATCTGATGTAAC+AGG 0.434311 tig0008586:+90443 None:intergenic
AACCTCAAGGAAAACAGTTC+TGG 0.464256 tig0008586:-90467 Msa1346280:CDS
GAAAGGTAAGGAGGCAATGC+AGG 0.491246 tig0008586:-90246 Msa1346280:CDS
ACACAAGAGGCAGCTAAAGA+TGG 0.492651 tig0008586:-90332 Msa1346280:CDS
AACGATGGTGCAGATAAGTT+TGG 0.500738 tig0008586:+90371 None:intergenic
AATGGATTGGACGGTCTTGT+TGG 0.505573 tig0008586:+90143 None:intergenic
CAGGCTCCTCCTCATCCACT+TGG 0.506515 tig0008586:-90227 Msa1346280:CDS
CACAAGATAGATGCATACTA+AGG 0.512296 tig0008586:-90865 Msa1346280:CDS
GGCTCCGATTTGTCAACTCC+AGG 0.517692 tig0008586:+90179 None:intergenic
ATGGAAGAAATGGATAATGC+TGG 0.520031 tig0008586:-90935 Msa1346280:CDS
GTTAACGGAAGTATGAAGTC+AGG 0.525847 tig0008586:-90290 Msa1346280:CDS
TAAGCCTGGAGTTGACAAAT+CGG 0.527134 tig0008586:-90183 Msa1346280:CDS
ACTGAGGAACCAAGTGGATG+AGG 0.533039 tig0008586:+90218 None:intergenic
GTAAAGTCGACAAATAAGCC+TGG 0.535418 tig0008586:-90197 Msa1346280:CDS
AAAATTCAATGACATATTGG+AGG 0.564152 tig0008586:-90621 Msa1346280:intron
GATGAAACAAGTGACAAGAA+AGG 0.573820 tig0008586:-90263 Msa1346280:CDS
GTTTCGGCCTTGCTACTGCT+AGG 0.582630 tig0008586:+90422 None:intergenic
AACAAGTGACAAGAAAGGTA+AGG 0.591817 tig0008586:-90258 Msa1346280:CDS
TACTCTACCTAGCAGTAGCA+AGG 0.601773 tig0008586:-90429 Msa1346280:CDS
GAGGAACCAAGTGGATGAGG+AGG 0.613107 tig0008586:+90221 None:intergenic
TCACTGGAGAAATCAATGAA+AGG 0.622381 tig0008586:-90689 Msa1346280:CDS
CTTATTTGTCGACTTTACTG+AGG 0.629325 tig0008586:+90202 None:intergenic
GACTACGGAAACCAACCTCA+AGG 0.639976 tig0008586:-90480 Msa1346280:CDS
AACGGAAGTATGAAGTCAGG+AGG 0.653379 tig0008586:-90287 Msa1346280:CDS
AAGTGACAAGAAAGGTAAGG+AGG 0.659061 tig0008586:-90255 Msa1346280:CDS
GACTTTACTGAGGAACCAAG+TGG 0.673337 tig0008586:+90212 None:intergenic
AAAATATATTCATTTCATCG+AGG 0.698984 tig0008586:+90112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATTAACAATCTTATA+CGG - tig0008586:90481-90500 Msa1346280:CDS 10.0%
!! TATATATTAACAATCTTATA+CGG - tig0008586:90481-90500 Msa1346280:CDS 10.0%
!! AATAGTATTCATATTTCAAA+TGG - tig0008586:90308-90327 Msa1346280:CDS 15.0%
!! TAAGATTGTTAATATATAGA+AGG + tig0008586:90479-90498 None:intergenic 15.0%
!! AATAGTATTCATATTTCAAA+TGG - tig0008586:90308-90327 Msa1346280:CDS 15.0%
!! TAAGATTGTTAATATATAGA+AGG + tig0008586:90479-90498 None:intergenic 15.0%
!!! ACTATTCTCTTTTATAATTC+AGG + tig0008586:90294-90313 None:intergenic 20.0%
!! ATATTTCAAATGGATATTTC+AGG - tig0008586:90318-90337 Msa1346280:CDS 20.0%
!!! CAATATGTCATTGAATTTTT+CGG + tig0008586:90448-90467 None:intergenic 20.0%
!!! TTATTTTTCTTTATGAACGA+TGG + tig0008586:90717-90736 None:intergenic 20.0%
!! AAAGAAAAATAAAACACAAG+AGG - tig0008586:90725-90744 Msa1346280:CDS 20.0%
!!! ACTATTCTCTTTTATAATTC+AGG + tig0008586:90294-90313 None:intergenic 20.0%
!! ATATTTCAAATGGATATTTC+AGG - tig0008586:90318-90337 Msa1346280:CDS 20.0%
!!! CAATATGTCATTGAATTTTT+CGG + tig0008586:90448-90467 None:intergenic 20.0%
!!! TTATTTTTCTTTATGAACGA+TGG + tig0008586:90717-90736 None:intergenic 20.0%
!! AAAGAAAAATAAAACACAAG+AGG - tig0008586:90725-90744 Msa1346280:CDS 20.0%
!!! TATAATTCAGGTTGCAATAA+AGG + tig0008586:90282-90301 None:intergenic 25.0%
! CGAAAAATTCAATGACATAT+TGG - tig0008586:90446-90465 Msa1346280:CDS 25.0%
! AAAATTCAATGACATATTGG+AGG - tig0008586:90449-90468 Msa1346280:CDS 25.0%
! AATAAACCTGCAAATGTTAA+CGG - tig0008586:90765-90784 Msa1346280:intron 25.0%
!!! TATAATTCAGGTTGCAATAA+AGG + tig0008586:90282-90301 None:intergenic 25.0%
! CGAAAAATTCAATGACATAT+TGG - tig0008586:90446-90465 Msa1346280:CDS 25.0%
! AAAATTCAATGACATATTGG+AGG - tig0008586:90449-90468 Msa1346280:CDS 25.0%
! AATAAACCTGCAAATGTTAA+CGG - tig0008586:90765-90784 Msa1346280:intron 25.0%
TCAGAAAAAGATGGAAGAAA+TGG - tig0008586:90125-90144 Msa1346280:CDS 30.0%
!! TTGAATTTTTCGGCCAAATT+TGG + tig0008586:90438-90457 None:intergenic 30.0%
TTCAAACACAAGGAATTACA+AGG + tig0008586:90540-90559 None:intergenic 30.0%
! TAATTCCTTGTGTTTGAAAC+AGG - tig0008586:90542-90561 Msa1346280:intron 30.0%
CAGTGAAGTTAAAAAGACTA+CGG - tig0008586:90575-90594 Msa1346280:intron 30.0%
AATAGATGCTTTCGATGTTT+CGG + tig0008586:90667-90686 None:intergenic 30.0%
TCAGAAAAAGATGGAAGAAA+TGG - tig0008586:90125-90144 Msa1346280:CDS 30.0%
!! TTGAATTTTTCGGCCAAATT+TGG + tig0008586:90438-90457 None:intergenic 30.0%
TTCAAACACAAGGAATTACA+AGG + tig0008586:90540-90559 None:intergenic 30.0%
! TAATTCCTTGTGTTTGAAAC+AGG - tig0008586:90542-90561 Msa1346280:intron 30.0%
CAGTGAAGTTAAAAAGACTA+CGG - tig0008586:90575-90594 Msa1346280:intron 30.0%
AATAGATGCTTTCGATGTTT+CGG + tig0008586:90667-90686 None:intergenic 30.0%
ATGGAAGAAATGGATAATGC+TGG - tig0008586:90135-90154 Msa1346280:CDS 35.0%
! AGAAATGGATAATGCTGGTT+TGG - tig0008586:90140-90159 Msa1346280:CDS 35.0%
! TGATGACGATGCTTTCAATT+TGG - tig0008586:90176-90195 Msa1346280:CDS 35.0%
CACAAGATAGATGCATACTA+AGG - tig0008586:90205-90224 Msa1346280:CDS 35.0%
TCACTGGAGAAATCAATGAA+AGG - tig0008586:90381-90400 Msa1346280:CDS 35.0%
!! AGCTCTGAAACTTCCAAATT+TGG - tig0008586:90422-90441 Msa1346280:CDS 35.0%
CAATCTTATACGGTACAGAA+AGG - tig0008586:90491-90510 Msa1346280:CDS 35.0%
ATACTTCCGTTAACATTTGC+AGG + tig0008586:90774-90793 None:intergenic 35.0%
GATGAAACAAGTGACAAGAA+AGG - tig0008586:90807-90826 Msa1346280:intron 35.0%
AACAAGTGACAAGAAAGGTA+AGG - tig0008586:90812-90831 Msa1346280:intron 35.0%
!! CTTATTTGTCGACTTTACTG+AGG + tig0008586:90871-90890 None:intergenic 35.0%
!! GACTTCAAAAATGGATTGGA+CGG + tig0008586:90939-90958 None:intergenic 35.0%
TTCATCGAGGACTTCAAAAA+TGG + tig0008586:90948-90967 None:intergenic 35.0%
ATGGAAGAAATGGATAATGC+TGG - tig0008586:90135-90154 Msa1346280:CDS 35.0%
! AGAAATGGATAATGCTGGTT+TGG - tig0008586:90140-90159 Msa1346280:CDS 35.0%
! TGATGACGATGCTTTCAATT+TGG - tig0008586:90176-90195 Msa1346280:CDS 35.0%
CACAAGATAGATGCATACTA+AGG - tig0008586:90205-90224 Msa1346280:CDS 35.0%
TCACTGGAGAAATCAATGAA+AGG - tig0008586:90381-90400 Msa1346280:CDS 35.0%
!! AGCTCTGAAACTTCCAAATT+TGG - tig0008586:90422-90441 Msa1346280:CDS 35.0%
CAATCTTATACGGTACAGAA+AGG - tig0008586:90491-90510 Msa1346280:CDS 35.0%
ATACTTCCGTTAACATTTGC+AGG + tig0008586:90774-90793 None:intergenic 35.0%
GATGAAACAAGTGACAAGAA+AGG - tig0008586:90807-90826 Msa1346280:intron 35.0%
AACAAGTGACAAGAAAGGTA+AGG - tig0008586:90812-90831 Msa1346280:intron 35.0%
!! CTTATTTGTCGACTTTACTG+AGG + tig0008586:90871-90890 None:intergenic 35.0%
!! GACTTCAAAAATGGATTGGA+CGG + tig0008586:90939-90958 None:intergenic 35.0%
TTCATCGAGGACTTCAAAAA+TGG + tig0008586:90948-90967 None:intergenic 35.0%
AGGTTGCAATAAAGGAAGAG+AGG + tig0008586:90274-90293 None:intergenic 40.0%
! ACTTGTCAAGCTTTTGTCAC+TGG - tig0008586:90365-90384 Msa1346280:CDS 40.0%
CCTTTCCTGTTTCAAACACA+AGG + tig0008586:90550-90569 None:intergenic 40.0%
! CCTTGTGTTTGAAACAGGAA+AGG - tig0008586:90547-90566 Msa1346280:intron 40.0%
! AGAACTGTTTTCCTTGAGGT+TGG + tig0008586:90604-90623 None:intergenic 40.0%
AACCTCAAGGAAAACAGTTC+TGG - tig0008586:90603-90622 Msa1346280:intron 40.0%
! GGTAGAGTATCTGATGTAAC+AGG + tig0008586:90630-90649 None:intergenic 40.0%
AACGATGGTGCAGATAAGTT+TGG + tig0008586:90702-90721 None:intergenic 40.0%
! GTTAACGGAAGTATGAAGTC+AGG - tig0008586:90780-90799 Msa1346280:intron 40.0%
AAGTGACAAGAAAGGTAAGG+AGG - tig0008586:90815-90834 Msa1346280:intron 40.0%
GTAAAGTCGACAAATAAGCC+TGG - tig0008586:90873-90892 Msa1346280:CDS 40.0%
TAAGCCTGGAGTTGACAAAT+CGG - tig0008586:90887-90906 Msa1346280:CDS 40.0%
CGAGGACTTCAAAAATGGAT+TGG + tig0008586:90943-90962 None:intergenic 40.0%
TGAAGTCCTCGATGAAATGA+AGG - tig0008586:90952-90971 Msa1346280:exon 40.0%
AGGTTGCAATAAAGGAAGAG+AGG + tig0008586:90274-90293 None:intergenic 40.0%
! ACTTGTCAAGCTTTTGTCAC+TGG - tig0008586:90365-90384 Msa1346280:CDS 40.0%
CCTTTCCTGTTTCAAACACA+AGG + tig0008586:90550-90569 None:intergenic 40.0%
! CCTTGTGTTTGAAACAGGAA+AGG - tig0008586:90547-90566 Msa1346280:intron 40.0%
! AGAACTGTTTTCCTTGAGGT+TGG + tig0008586:90604-90623 None:intergenic 40.0%
AACCTCAAGGAAAACAGTTC+TGG - tig0008586:90603-90622 Msa1346280:intron 40.0%
! GGTAGAGTATCTGATGTAAC+AGG + tig0008586:90630-90649 None:intergenic 40.0%
AACGATGGTGCAGATAAGTT+TGG + tig0008586:90702-90721 None:intergenic 40.0%
! GTTAACGGAAGTATGAAGTC+AGG - tig0008586:90780-90799 Msa1346280:intron 40.0%
AAGTGACAAGAAAGGTAAGG+AGG - tig0008586:90815-90834 Msa1346280:intron 40.0%
GTAAAGTCGACAAATAAGCC+TGG - tig0008586:90873-90892 Msa1346280:CDS 40.0%
TAAGCCTGGAGTTGACAAAT+CGG - tig0008586:90887-90906 Msa1346280:CDS 40.0%
CGAGGACTTCAAAAATGGAT+TGG + tig0008586:90943-90962 None:intergenic 40.0%
! GTCCAGAACTGTTTTCCTTG+AGG + tig0008586:90608-90627 None:intergenic 45.0%
TACTCTACCTAGCAGTAGCA+AGG - tig0008586:90641-90660 Msa1346280:CDS 45.0%
ACACAAGAGGCAGCTAAAGA+TGG - tig0008586:90738-90757 Msa1346280:intron 45.0%
! AACGGAAGTATGAAGTCAGG+AGG - tig0008586:90783-90802 Msa1346280:intron 45.0%
GACTTTACTGAGGAACCAAG+TGG + tig0008586:90861-90880 None:intergenic 45.0%
TGACAAATCGGAGCCTAGTT+TGG - tig0008586:90899-90918 Msa1346280:CDS 45.0%
!! CTTGTTGGTGTCACCAAACT+AGG + tig0008586:90915-90934 None:intergenic 45.0%
!! AATGGATTGGACGGTCTTGT+TGG + tig0008586:90930-90949 None:intergenic 45.0%
! GTCCAGAACTGTTTTCCTTG+AGG + tig0008586:90608-90627 None:intergenic 45.0%
TACTCTACCTAGCAGTAGCA+AGG - tig0008586:90641-90660 Msa1346280:CDS 45.0%
ACACAAGAGGCAGCTAAAGA+TGG - tig0008586:90738-90757 Msa1346280:intron 45.0%
! AACGGAAGTATGAAGTCAGG+AGG - tig0008586:90783-90802 Msa1346280:intron 45.0%
GACTTTACTGAGGAACCAAG+TGG + tig0008586:90861-90880 None:intergenic 45.0%
TGACAAATCGGAGCCTAGTT+TGG - tig0008586:90899-90918 Msa1346280:CDS 45.0%
!! CTTGTTGGTGTCACCAAACT+AGG + tig0008586:90915-90934 None:intergenic 45.0%
!! AATGGATTGGACGGTCTTGT+TGG + tig0008586:90930-90949 None:intergenic 45.0%
GACTACGGAAACCAACCTCA+AGG - tig0008586:90590-90609 Msa1346280:intron 50.0%
GAAAGGTAAGGAGGCAATGC+AGG - tig0008586:90824-90843 Msa1346280:intron 50.0%
ACTGAGGAACCAAGTGGATG+AGG + tig0008586:90855-90874 None:intergenic 50.0%
GACTACGGAAACCAACCTCA+AGG - tig0008586:90590-90609 Msa1346280:intron 50.0%
GAAAGGTAAGGAGGCAATGC+AGG - tig0008586:90824-90843 Msa1346280:intron 50.0%
ACTGAGGAACCAAGTGGATG+AGG + tig0008586:90855-90874 None:intergenic 50.0%
GTTTCGGCCTTGCTACTGCT+AGG + tig0008586:90651-90670 None:intergenic 55.0%
GAGGAACCAAGTGGATGAGG+AGG + tig0008586:90852-90871 None:intergenic 55.0%
! GGCTCCGATTTGTCAACTCC+AGG + tig0008586:90894-90913 None:intergenic 55.0%
GTTTCGGCCTTGCTACTGCT+AGG + tig0008586:90651-90670 None:intergenic 55.0%
GAGGAACCAAGTGGATGAGG+AGG + tig0008586:90852-90871 None:intergenic 55.0%
! GGCTCCGATTTGTCAACTCC+AGG + tig0008586:90894-90913 None:intergenic 55.0%
CAGGCTCCTCCTCATCCACT+TGG - tig0008586:90843-90862 Msa1346280:CDS 60.0%
CAGGCTCCTCCTCATCCACT+TGG - tig0008586:90843-90862 Msa1346280:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0008586 gene 90121 90971 90121 ID=Msa1346280;Name=Msa1346280
tig0008586 mRNA 90121 90971 90121 ID=Msa1346280-mRNA-1;Parent=Msa1346280;Name=Msa1346280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=14|1|0.66|1|1|1|3|0|217
tig0008586 exon 90121 90528 90121 ID=Msa1346280-mRNA-1:exon:5432;Parent=Msa1346280-mRNA-1
tig0008586 exon 90622 90752 90622 ID=Msa1346280-mRNA-1:exon:5431;Parent=Msa1346280-mRNA-1
tig0008586 exon 90843 90971 90843 ID=Msa1346280-mRNA-1:exon:5430;Parent=Msa1346280-mRNA-1
tig0008586 five_prime_UTR 90958 90971 90958 ID=Msa1346280-mRNA-1:five_prime_utr;Parent=Msa1346280-mRNA-1
tig0008586 CDS 90843 90957 90843 ID=Msa1346280-mRNA-1:cds;Parent=Msa1346280-mRNA-1
tig0008586 CDS 90622 90752 90622 ID=Msa1346280-mRNA-1:cds;Parent=Msa1346280-mRNA-1
tig0008586 CDS 90121 90528 90121 ID=Msa1346280-mRNA-1:cds;Parent=Msa1346280-mRNA-1
Gene Sequence

>Msa1346280

ATGGAAGAAATGGATAATGCTGGTTTGGATGCTAGTTTACTTGATGACGATGCTTTCAATTTGGAAGCAGCACAAGATAGATGCATACTAAGGCTAATAGCATCGTGCTGCAATAGTGATAAACTTGTGAGAGCTACTGAACTTGTCAAGCTTTTGTCACTGGAGAAATCAATGAAAGGTGCAATAAAGCTTGTTACAGCTCTGAAACTTCCAAATTTGGCCGAAAAATTCAATGACATATTGGAGGAAAGGCTATACAGTGAAGTTAAAAAGACTACGGAAACCAACCTCAAGGAAAACAGTTCTGGACCTGTTACATCAGATACTCTACCTAGCAGTAGCAAGGCCGAAACATCGAAAGCATCTATTATTTCATCATCACCAAACTTATCTGCACCATCGTTCATAAAGAAAAATAAAACACAAGAGGCAGCTAAAGATGGAATGAATAAACCTGCAAATGTTAACGGAAGTATGAAGTCAGGAGGAGATGAAACAAGTGACAAGAAAGGTAAGGAGGCAATGCAGGCTCCTCCTCATCCACTTGGTTCCTCAGTAAAGTCGACAAATAAGCCTGGAGTTGACAAATCGGAGCCTAGTTTGGTGACACCAACAAGACCGTCCAATCCATTTTTGAAGTCCTCGATGAAATGA

Protein sequence

>Msa1346280

MEEMDNAGLDASLLDDDAFNLEAAQDRCILRLIASCCNSDKLVRATELVKLLSLEKSMKGAIKLVTALKLPNLAEKFNDILEERLYSEVKKTTETNLKENSSGPVTSDTLPSSSKAETSKASIISSSPNLSAPSFIKKNKTQEAAKDGMNKPANVNGSMKSGGDETSDKKGKEAMQAPPHPLGSSVKSTNKPGVDKSEPSLVTPTRPSNPFLKSSMK*