AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0422100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0422100 MtrunA17_Chr4g0069091 88.636 44 5 0 9 52 932 975 4.12e-19 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACGACTTCCCTTGTTTC+AGG 0.153942 3_2:+71233552 None:intergenic
AGAACCCTCTATTTACAATT+TGG 0.213420 3_2:+71233972 None:intergenic
TTTAACCAAATTGTAAATAG+AGG 0.287628 3_2:-71233977 Msa0422100:five_prime_UTR
GTTGAGAAAATTAATATGTT+TGG 0.325329 3_2:-71232948 Msa0422100:CDS
AAACTGCTTCACTTCACTAA+TGG 0.340507 3_2:-71233516 Msa0422100:CDS
TTGTAATTCTTTCAACTGAA+TGG 0.353975 3_2:+71233487 None:intergenic
ATTTACAATTTGGTTAAACT+TGG 0.377532 3_2:+71233982 None:intergenic
CTGGAAAATTCTACAGAAAA+CGG 0.382177 3_2:-71233764 Msa0422100:five_prime_UTR
TTCTCCCTCTCATTTGTCCA+TGG 0.403548 3_2:+71233700 None:intergenic
AAATAAAATTAGATTGGATC+TGG 0.410449 3_2:-71233783 Msa0422100:five_prime_UTR
ATCTCTGTCAACAACGGAAA+TGG 0.410536 3_2:-71233847 Msa0422100:five_prime_UTR
AAATCTAAATAAAATTAGAT+TGG 0.429845 3_2:-71233789 Msa0422100:five_prime_UTR
GAGACAACGTTGGTGGGACT+TGG 0.440066 3_2:+71234056 None:intergenic
TGGAAAATTCTACAGAAAAC+GGG 0.448752 3_2:-71233763 Msa0422100:five_prime_UTR
GGATGAAGATGGCGTGGATG+AGG 0.450681 3_2:-71233441 Msa0422100:CDS
ATTAACCTCATCATAATCTT+CGG 0.465947 3_2:+71233400 None:intergenic
AGCTTTACTTGCTGTCTCCA+AGG 0.485487 3_2:-71232913 None:intergenic
ACAAATCCTCATCTATCTGA+TGG 0.493937 3_2:-71234022 Msa0422100:five_prime_UTR
TGGGAAAGGAGATGAAGTAG+AGG 0.496213 3_2:-71233462 Msa0422100:CDS
TTCTCCACTAAAATCTCAAA+AGG 0.501636 3_2:+71233881 None:intergenic
TTAACCAAATTGTAAATAGA+GGG 0.513442 3_2:-71233976 Msa0422100:five_prime_UTR
TTTACAATTTGGTTAAACTT+GGG 0.520147 3_2:+71233983 None:intergenic
TTGGAGACAGCAAGTAAAGC+TGG 0.531827 3_2:+71232915 None:intergenic
CAGTTGAAAGAATTACAAGA+TGG 0.531894 3_2:-71233482 Msa0422100:CDS
TGAGGATTTGTGAGAACATG+AGG 0.537057 3_2:+71234034 None:intergenic
GATGAAGTAGAGGATGAAGA+TGG 0.543029 3_2:-71233452 Msa0422100:CDS
CTGAGCAACTCCTGAAACAA+GGG 0.557999 3_2:-71233562 Msa0422100:CDS
AGTTGAAAGAATTACAAGAT+GGG 0.566152 3_2:-71233481 Msa0422100:CDS
CTTCTCCACACAAACCTCCA+TGG 0.568447 3_2:+71233673 None:intergenic
AGACAACGTTGGTGGGACTT+GGG 0.570867 3_2:+71234057 None:intergenic
GCAACAGAGGAAAGGAATTG+AGG 0.575282 3_2:-71233823 Msa0422100:five_prime_UTR
ACTGAGCAACTCCTGAAACA+AGG 0.580659 3_2:-71233563 Msa0422100:CDS
CATGAGGAGACAACGTTGGT+GGG 0.583958 3_2:+71234050 None:intergenic
AAAGAATTACAAGATGGGAA+AGG 0.585242 3_2:-71233476 Msa0422100:CDS
ACATGAGGAGACAACGTTGG+TGG 0.586011 3_2:+71234049 None:intergenic
CTGCTTCACTTCACTAATGG+AGG 0.589139 3_2:-71233513 Msa0422100:CDS
GAAATGGTGCAACAGAGGAA+AGG 0.595921 3_2:-71233831 Msa0422100:five_prime_UTR
ATGAGAGGGAGAATGAGCCA+TGG 0.616733 3_2:-71233690 Msa0422100:CDS
CAACGGAAATGGTGCAACAG+AGG 0.619098 3_2:-71233836 Msa0422100:five_prime_UTR
AAAAGCCATGGACAAATGAG+AGG 0.628002 3_2:-71233705 Msa0422100:exon
TTTGTTATCTCTGTCAACAA+CGG 0.631335 3_2:-71233853 Msa0422100:five_prime_UTR
AGATTTCCATCAGATAGATG+AGG 0.634347 3_2:+71234016 None:intergenic
AGTAGAGGATGAAGATGGCG+TGG 0.645857 3_2:-71233447 Msa0422100:CDS
AGAGGGAGAATGAGCCATGG+AGG 0.663372 3_2:-71233687 Msa0422100:intron
CTGAAACAAGGGAAGTCGTG+TGG 0.664589 3_2:-71233551 Msa0422100:CDS
AAAGCCATGGACAAATGAGA+GGG 0.669427 3_2:-71233704 Msa0422100:exon
TGACTCCGAAGATTATGATG+AGG 0.676696 3_2:-71233405 Msa0422100:intron
AGAACATGAGGAGACAACGT+TGG 0.772358 3_2:+71234046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTAAATAAAATTAGAT+TGG - chr3_2:71233190-71233209 Msa0422100:intron 10.0%
!! AAATCTAAATAAAATTAGAT+TGG - chr3_2:71233190-71233209 Msa0422100:intron 10.0%
!! AAATAACAAAAATATTGAGA+AGG + chr3_2:71233897-71233916 None:intergenic 15.0%
!!! TTCTCAATATTTTTGTTATT+TGG - chr3_2:71233896-71233915 Msa0422100:five_prime_UTR 15.0%
!! AAATAACAAAAATATTGAGA+AGG + chr3_2:71233897-71233916 None:intergenic 15.0%
!!! TTCTCAATATTTTTGTTATT+TGG - chr3_2:71233896-71233915 Msa0422100:five_prime_UTR 15.0%
!! TTTACAATTTGGTTAAACTT+GGG + chr3_2:71232999-71233018 None:intergenic 20.0%
!! ATTTACAATTTGGTTAAACT+TGG + chr3_2:71233000-71233019 None:intergenic 20.0%
!! TTTAACCAAATTGTAAATAG+AGG - chr3_2:71233002-71233021 Msa0422100:intron 20.0%
!! TTAACCAAATTGTAAATAGA+GGG - chr3_2:71233003-71233022 Msa0422100:intron 20.0%
!! AAATAAAATTAGATTGGATC+TGG - chr3_2:71233196-71233215 Msa0422100:intron 20.0%
!!! CAATTTTATTAGTTGTATCT+TGG - chr3_2:71233928-71233947 Msa0422100:five_prime_UTR 20.0%
!! GTTGAGAAAATTAATATGTT+TGG - chr3_2:71234031-71234050 Msa0422100:five_prime_UTR 20.0%
!! TTTACAATTTGGTTAAACTT+GGG + chr3_2:71232999-71233018 None:intergenic 20.0%
!! ATTTACAATTTGGTTAAACT+TGG + chr3_2:71233000-71233019 None:intergenic 20.0%
!! TTTAACCAAATTGTAAATAG+AGG - chr3_2:71233002-71233021 Msa0422100:intron 20.0%
!! TTAACCAAATTGTAAATAGA+GGG - chr3_2:71233003-71233022 Msa0422100:intron 20.0%
!! AAATAAAATTAGATTGGATC+TGG - chr3_2:71233196-71233215 Msa0422100:intron 20.0%
!!! CAATTTTATTAGTTGTATCT+TGG - chr3_2:71233928-71233947 Msa0422100:five_prime_UTR 20.0%
!! GTTGAGAAAATTAATATGTT+TGG - chr3_2:71234031-71234050 Msa0422100:five_prime_UTR 20.0%
! TCTTAACTACAAACCAAAAA+CGG - chr3_2:71233240-71233259 Msa0422100:intron 25.0%
! TTGTAATTCTTTCAACTGAA+TGG + chr3_2:71233495-71233514 None:intergenic 25.0%
! AGTTGAAAGAATTACAAGAT+GGG - chr3_2:71233498-71233517 Msa0422100:CDS 25.0%
! ATTAACCTCATCATAATCTT+CGG + chr3_2:71233582-71233601 None:intergenic 25.0%
! GGTTGAAGAATATGAATAAA+CGG - chr3_2:71233761-71233780 Msa0422100:five_prime_UTR 25.0%
! TTATTAGTTGTATCTTGGTT+CGG - chr3_2:71233933-71233952 Msa0422100:five_prime_UTR 25.0%
! TCTTAACTACAAACCAAAAA+CGG - chr3_2:71233240-71233259 Msa0422100:intron 25.0%
! TTGTAATTCTTTCAACTGAA+TGG + chr3_2:71233495-71233514 None:intergenic 25.0%
! AGTTGAAAGAATTACAAGAT+GGG - chr3_2:71233498-71233517 Msa0422100:CDS 25.0%
! ATTAACCTCATCATAATCTT+CGG + chr3_2:71233582-71233601 None:intergenic 25.0%
! GGTTGAAGAATATGAATAAA+CGG - chr3_2:71233761-71233780 Msa0422100:five_prime_UTR 25.0%
! TTATTAGTTGTATCTTGGTT+CGG - chr3_2:71233933-71233952 Msa0422100:five_prime_UTR 25.0%
AGAACCCTCTATTTACAATT+TGG + chr3_2:71233010-71233029 None:intergenic 30.0%
!!! TTTGCCTTTTGAGATTTTAG+TGG - chr3_2:71233094-71233113 Msa0422100:intron 30.0%
TTCTCCACTAAAATCTCAAA+AGG + chr3_2:71233101-71233120 None:intergenic 30.0%
! TTTGTTATCTCTGTCAACAA+CGG - chr3_2:71233126-71233145 Msa0422100:intron 30.0%
CTGGAAAATTCTACAGAAAA+CGG - chr3_2:71233215-71233234 Msa0422100:intron 30.0%
TGGAAAATTCTACAGAAAAC+GGG - chr3_2:71233216-71233235 Msa0422100:intron 30.0%
AAACCAAAAACGGAAAATTC+TGG - chr3_2:71233250-71233269 Msa0422100:intron 30.0%
! TTTCCAGAATTTTCCGTTTT+TGG + chr3_2:71233256-71233275 None:intergenic 30.0%
!! TAACTTTTCCGTTTTAGTGA+TGG - chr3_2:71233379-71233398 Msa0422100:intron 30.0%
CAGTTGAAAGAATTACAAGA+TGG - chr3_2:71233497-71233516 Msa0422100:CDS 30.0%
AAAGAATTACAAGATGGGAA+AGG - chr3_2:71233503-71233522 Msa0422100:CDS 30.0%
! TATATCTCCTTTTGTCTACA+AGG - chr3_2:71233649-71233668 Msa0422100:intron 30.0%
TTTAACAGTTCTTAATCCTG+TGG - chr3_2:71233715-71233734 Msa0422100:five_prime_UTR 30.0%
TTAACAGTTCTTAATCCTGT+GGG - chr3_2:71233716-71233735 Msa0422100:five_prime_UTR 30.0%
TAAACGGAAGAAGAATTTCT+TGG - chr3_2:71233777-71233796 Msa0422100:five_prime_UTR 30.0%
CAATAACTGGATATGGAATT+AGG - chr3_2:71233867-71233886 Msa0422100:five_prime_UTR 30.0%
AGAACCCTCTATTTACAATT+TGG + chr3_2:71233010-71233029 None:intergenic 30.0%
!!! TTTGCCTTTTGAGATTTTAG+TGG - chr3_2:71233094-71233113 Msa0422100:intron 30.0%
TTCTCCACTAAAATCTCAAA+AGG + chr3_2:71233101-71233120 None:intergenic 30.0%
! TTTGTTATCTCTGTCAACAA+CGG - chr3_2:71233126-71233145 Msa0422100:intron 30.0%
CTGGAAAATTCTACAGAAAA+CGG - chr3_2:71233215-71233234 Msa0422100:intron 30.0%
TGGAAAATTCTACAGAAAAC+GGG - chr3_2:71233216-71233235 Msa0422100:intron 30.0%
AAACCAAAAACGGAAAATTC+TGG - chr3_2:71233250-71233269 Msa0422100:intron 30.0%
! TTTCCAGAATTTTCCGTTTT+TGG + chr3_2:71233256-71233275 None:intergenic 30.0%
!! TAACTTTTCCGTTTTAGTGA+TGG - chr3_2:71233379-71233398 Msa0422100:intron 30.0%
CAGTTGAAAGAATTACAAGA+TGG - chr3_2:71233497-71233516 Msa0422100:CDS 30.0%
AAAGAATTACAAGATGGGAA+AGG - chr3_2:71233503-71233522 Msa0422100:CDS 30.0%
! TATATCTCCTTTTGTCTACA+AGG - chr3_2:71233649-71233668 Msa0422100:intron 30.0%
TTTAACAGTTCTTAATCCTG+TGG - chr3_2:71233715-71233734 Msa0422100:five_prime_UTR 30.0%
TTAACAGTTCTTAATCCTGT+GGG - chr3_2:71233716-71233735 Msa0422100:five_prime_UTR 30.0%
TAAACGGAAGAAGAATTTCT+TGG - chr3_2:71233777-71233796 Msa0422100:five_prime_UTR 30.0%
CAATAACTGGATATGGAATT+AGG - chr3_2:71233867-71233886 Msa0422100:five_prime_UTR 30.0%
ACAAATCCTCATCTATCTGA+TGG - chr3_2:71232957-71232976 Msa0422100:CDS 35.0%
AGATTTCCATCAGATAGATG+AGG + chr3_2:71232966-71232985 None:intergenic 35.0%
! GAAAATTCTGGAAAAAGCCA+TGG - chr3_2:71233262-71233281 Msa0422100:intron 35.0%
AAACTGCTTCACTTCACTAA+TGG - chr3_2:71233463-71233482 Msa0422100:CDS 35.0%
CTGTTTAAATTAGGACTCTG+AGG - chr3_2:71233740-71233759 Msa0422100:five_prime_UTR 35.0%
! GATGGTTCAGTTTTCACAAA+AGG - chr3_2:71233827-71233846 Msa0422100:five_prime_UTR 35.0%
! GTTCAGTTTTCACAAAAGGA+GGG - chr3_2:71233831-71233850 Msa0422100:five_prime_UTR 35.0%
! TTCAGTTTTCACAAAAGGAG+GGG - chr3_2:71233832-71233851 Msa0422100:five_prime_UTR 35.0%
CGAAGATCAATAACTGGATA+TGG - chr3_2:71233860-71233879 Msa0422100:five_prime_UTR 35.0%
ACAAATCCTCATCTATCTGA+TGG - chr3_2:71232957-71232976 Msa0422100:CDS 35.0%
AGATTTCCATCAGATAGATG+AGG + chr3_2:71232966-71232985 None:intergenic 35.0%
! GAAAATTCTGGAAAAAGCCA+TGG - chr3_2:71233262-71233281 Msa0422100:intron 35.0%
AAACTGCTTCACTTCACTAA+TGG - chr3_2:71233463-71233482 Msa0422100:CDS 35.0%
CTGTTTAAATTAGGACTCTG+AGG - chr3_2:71233740-71233759 Msa0422100:five_prime_UTR 35.0%
! GATGGTTCAGTTTTCACAAA+AGG - chr3_2:71233827-71233846 Msa0422100:five_prime_UTR 35.0%
! GTTCAGTTTTCACAAAAGGA+GGG - chr3_2:71233831-71233850 Msa0422100:five_prime_UTR 35.0%
! TTCAGTTTTCACAAAAGGAG+GGG - chr3_2:71233832-71233851 Msa0422100:five_prime_UTR 35.0%
CGAAGATCAATAACTGGATA+TGG - chr3_2:71233860-71233879 Msa0422100:five_prime_UTR 35.0%
TGAGGATTTGTGAGAACATG+AGG + chr3_2:71232948-71232967 None:intergenic 40.0%
ATCTCTGTCAACAACGGAAA+TGG - chr3_2:71233132-71233151 Msa0422100:intron 40.0%
AAAAGCCATGGACAAATGAG+AGG - chr3_2:71233274-71233293 Msa0422100:intron 40.0%
AAAGCCATGGACAAATGAGA+GGG - chr3_2:71233275-71233294 Msa0422100:intron 40.0%
ACCTAGTGCCATCACTAAAA+CGG + chr3_2:71233390-71233409 None:intergenic 40.0%
GATGAAGTAGAGGATGAAGA+TGG - chr3_2:71233527-71233546 Msa0422100:CDS 40.0%
TGACTCCGAAGATTATGATG+AGG - chr3_2:71233574-71233593 Msa0422100:CDS 40.0%
CACAGAACCTTGTAGACAAA+AGG + chr3_2:71233659-71233678 None:intergenic 40.0%
CCTAATTTAAACAGACCCAC+AGG + chr3_2:71233734-71233753 None:intergenic 40.0%
CCTGTGGGTCTGTTTAAATT+AGG - chr3_2:71233731-71233750 Msa0422100:five_prime_UTR 40.0%
! GGTTCAGTTTTCACAAAAGG+AGG - chr3_2:71233830-71233849 Msa0422100:five_prime_UTR 40.0%
! TCAGTTTTCACAAAAGGAGG+GGG - chr3_2:71233833-71233852 Msa0422100:five_prime_UTR 40.0%
GGATGTCGAAGATCAATAAC+TGG - chr3_2:71233854-71233873 Msa0422100:five_prime_UTR 40.0%
TGAGGATTTGTGAGAACATG+AGG + chr3_2:71232948-71232967 None:intergenic 40.0%
ATCTCTGTCAACAACGGAAA+TGG - chr3_2:71233132-71233151 Msa0422100:intron 40.0%
AAAAGCCATGGACAAATGAG+AGG - chr3_2:71233274-71233293 Msa0422100:intron 40.0%
AAAGCCATGGACAAATGAGA+GGG - chr3_2:71233275-71233294 Msa0422100:intron 40.0%
ACCTAGTGCCATCACTAAAA+CGG + chr3_2:71233390-71233409 None:intergenic 40.0%
GATGAAGTAGAGGATGAAGA+TGG - chr3_2:71233527-71233546 Msa0422100:CDS 40.0%
TGACTCCGAAGATTATGATG+AGG - chr3_2:71233574-71233593 Msa0422100:CDS 40.0%
CACAGAACCTTGTAGACAAA+AGG + chr3_2:71233659-71233678 None:intergenic 40.0%
CCTAATTTAAACAGACCCAC+AGG + chr3_2:71233734-71233753 None:intergenic 40.0%
CCTGTGGGTCTGTTTAAATT+AGG - chr3_2:71233731-71233750 Msa0422100:five_prime_UTR 40.0%
! GGTTCAGTTTTCACAAAAGG+AGG - chr3_2:71233830-71233849 Msa0422100:five_prime_UTR 40.0%
! TCAGTTTTCACAAAAGGAGG+GGG - chr3_2:71233833-71233852 Msa0422100:five_prime_UTR 40.0%
GGATGTCGAAGATCAATAAC+TGG - chr3_2:71233854-71233873 Msa0422100:five_prime_UTR 40.0%
AGAACATGAGGAGACAACGT+TGG + chr3_2:71232936-71232955 None:intergenic 45.0%
GAAATGGTGCAACAGAGGAA+AGG - chr3_2:71233148-71233167 Msa0422100:intron 45.0%
GCAACAGAGGAAAGGAATTG+AGG - chr3_2:71233156-71233175 Msa0422100:intron 45.0%
TTCTCCCTCTCATTTGTCCA+TGG + chr3_2:71233282-71233301 None:intergenic 45.0%
!! TCCGTTTTAGTGATGGCACT+AGG - chr3_2:71233386-71233405 Msa0422100:intron 45.0%
ACTGAGCAACTCCTGAAACA+AGG - chr3_2:71233416-71233435 Msa0422100:CDS 45.0%
CTGAGCAACTCCTGAAACAA+GGG - chr3_2:71233417-71233436 Msa0422100:CDS 45.0%
CTGCTTCACTTCACTAATGG+AGG - chr3_2:71233466-71233485 Msa0422100:CDS 45.0%
TGGGAAAGGAGATGAAGTAG+AGG - chr3_2:71233517-71233536 Msa0422100:CDS 45.0%
TTGGAGACAGCAAGTAAAGC+TGG + chr3_2:71234067-71234086 None:intergenic 45.0%
!! AGCTTTACTTGCTGTCTCCA+AGG - chr3_2:71234066-71234085 Msa0422100:five_prime_UTR 45.0%
AGAACATGAGGAGACAACGT+TGG + chr3_2:71232936-71232955 None:intergenic 45.0%
GAAATGGTGCAACAGAGGAA+AGG - chr3_2:71233148-71233167 Msa0422100:intron 45.0%
GCAACAGAGGAAAGGAATTG+AGG - chr3_2:71233156-71233175 Msa0422100:intron 45.0%
TTCTCCCTCTCATTTGTCCA+TGG + chr3_2:71233282-71233301 None:intergenic 45.0%
!! TCCGTTTTAGTGATGGCACT+AGG - chr3_2:71233386-71233405 Msa0422100:intron 45.0%
ACTGAGCAACTCCTGAAACA+AGG - chr3_2:71233416-71233435 Msa0422100:CDS 45.0%
CTGAGCAACTCCTGAAACAA+GGG - chr3_2:71233417-71233436 Msa0422100:CDS 45.0%
CTGCTTCACTTCACTAATGG+AGG - chr3_2:71233466-71233485 Msa0422100:CDS 45.0%
TGGGAAAGGAGATGAAGTAG+AGG - chr3_2:71233517-71233536 Msa0422100:CDS 45.0%
TTGGAGACAGCAAGTAAAGC+TGG + chr3_2:71234067-71234086 None:intergenic 45.0%
! AGACAACGTTGGTGGGACTT+GGG + chr3_2:71232925-71232944 None:intergenic 50.0%
CATGAGGAGACAACGTTGGT+GGG + chr3_2:71232932-71232951 None:intergenic 50.0%
ACATGAGGAGACAACGTTGG+TGG + chr3_2:71232933-71232952 None:intergenic 50.0%
CAACGGAAATGGTGCAACAG+AGG - chr3_2:71233143-71233162 Msa0422100:intron 50.0%
ATGAGAGGGAGAATGAGCCA+TGG - chr3_2:71233289-71233308 Msa0422100:intron 50.0%
ATGAGCCATGGAGGTTTGTG+TGG - chr3_2:71233301-71233320 Msa0422100:intron 50.0%
CTTCTCCACACAAACCTCCA+TGG + chr3_2:71233309-71233328 None:intergenic 50.0%
! CACACGACTTCCCTTGTTTC+AGG + chr3_2:71233430-71233449 None:intergenic 50.0%
CTGAAACAAGGGAAGTCGTG+TGG - chr3_2:71233428-71233447 Msa0422100:CDS 50.0%
AGTAGAGGATGAAGATGGCG+TGG - chr3_2:71233532-71233551 Msa0422100:CDS 50.0%
CTTGGTATGCTAAAGCTGCC+CGG - chr3_2:71233795-71233814 Msa0422100:five_prime_UTR 50.0%
TTGGTATGCTAAAGCTGCCC+GGG - chr3_2:71233796-71233815 Msa0422100:five_prime_UTR 50.0%
TGAACCATCTTCCAATGCCC+GGG + chr3_2:71233816-71233835 None:intergenic 50.0%
CTGAACCATCTTCCAATGCC+CGG + chr3_2:71233817-71233836 None:intergenic 50.0%
! AGACAACGTTGGTGGGACTT+GGG + chr3_2:71232925-71232944 None:intergenic 50.0%
CATGAGGAGACAACGTTGGT+GGG + chr3_2:71232932-71232951 None:intergenic 50.0%
ACATGAGGAGACAACGTTGG+TGG + chr3_2:71232933-71232952 None:intergenic 50.0%
CAACGGAAATGGTGCAACAG+AGG - chr3_2:71233143-71233162 Msa0422100:intron 50.0%
ATGAGAGGGAGAATGAGCCA+TGG - chr3_2:71233289-71233308 Msa0422100:intron 50.0%
ATGAGCCATGGAGGTTTGTG+TGG - chr3_2:71233301-71233320 Msa0422100:intron 50.0%
CTTCTCCACACAAACCTCCA+TGG + chr3_2:71233309-71233328 None:intergenic 50.0%
! CACACGACTTCCCTTGTTTC+AGG + chr3_2:71233430-71233449 None:intergenic 50.0%
CTGAAACAAGGGAAGTCGTG+TGG - chr3_2:71233428-71233447 Msa0422100:CDS 50.0%
AGTAGAGGATGAAGATGGCG+TGG - chr3_2:71233532-71233551 Msa0422100:CDS 50.0%
CTTGGTATGCTAAAGCTGCC+CGG - chr3_2:71233795-71233814 Msa0422100:five_prime_UTR 50.0%
TTGGTATGCTAAAGCTGCCC+GGG - chr3_2:71233796-71233815 Msa0422100:five_prime_UTR 50.0%
TGAACCATCTTCCAATGCCC+GGG + chr3_2:71233816-71233835 None:intergenic 50.0%
CTGAACCATCTTCCAATGCC+CGG + chr3_2:71233817-71233836 None:intergenic 50.0%
! GAGACAACGTTGGTGGGACT+TGG + chr3_2:71232926-71232945 None:intergenic 55.0%
AGAGGGAGAATGAGCCATGG+AGG - chr3_2:71233292-71233311 Msa0422100:intron 55.0%
GGATGAAGATGGCGTGGATG+AGG - chr3_2:71233538-71233557 Msa0422100:CDS 55.0%
! GAGACAACGTTGGTGGGACT+TGG + chr3_2:71232926-71232945 None:intergenic 55.0%
AGAGGGAGAATGAGCCATGG+AGG - chr3_2:71233292-71233311 Msa0422100:intron 55.0%
GGATGAAGATGGCGTGGATG+AGG - chr3_2:71233538-71233557 Msa0422100:CDS 55.0%
TGCTAAAGCTGCCCGGGCAT+TGG - chr3_2:71233802-71233821 Msa0422100:five_prime_UTR 60.0%
TGCTAAAGCTGCCCGGGCAT+TGG - chr3_2:71233802-71233821 Msa0422100:five_prime_UTR 60.0%
!! GCTGCCCGGGCATTGGAAGA+TGG - chr3_2:71233809-71233828 Msa0422100:five_prime_UTR 65.0%
!! GCTGCCCGGGCATTGGAAGA+TGG - chr3_2:71233809-71233828 Msa0422100:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 71232914 71234087 71232914 ID=Msa0422100;Name=Msa0422100
chr3_2 mRNA 71232914 71234087 71232914 ID=Msa0422100-mRNA-1;Parent=Msa0422100;Name=Msa0422100-mRNA-1;_AED=0.28;_eAED=0.35;_QI=375|0.5|0.66|1|1|1|3|0|98
chr3_2 exon 71232914 71232982 71232914 ID=Msa0422100-mRNA-1:exon:17375;Parent=Msa0422100-mRNA-1
chr3_2 exon 71233406 71233605 71233406 ID=Msa0422100-mRNA-1:exon:17374;Parent=Msa0422100-mRNA-1
chr3_2 exon 71233688 71234087 71233688 ID=Msa0422100-mRNA-1:exon:17373;Parent=Msa0422100-mRNA-1
chr3_2 five_prime_UTR 71233713 71234087 71233713 ID=Msa0422100-mRNA-1:five_prime_utr;Parent=Msa0422100-mRNA-1
chr3_2 CDS 71233688 71233712 71233688 ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1
chr3_2 CDS 71233406 71233605 71233406 ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1
chr3_2 CDS 71232914 71232982 71232914 ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1
Gene Sequence

>Msa0422100

ATGAGAGGGAGAATGAGCCATGGAGTGATGGCACTAGGTCGATTGACTGAGCAACTCCTGAAACAAGGGAAGTCGTGTGGTGATTCAATTCAAAACTGCTTCACTTCACTAATGGAGGTATCCATTCAGTTGAAAGAATTACAAGATGGGAAAGGAGATGAAGTAGAGGATGAAGATGGCGTGGATGAGGATAGTGACGATGATGACTCCGAAGATTATGATGAGAAAATTAATGATGTTGAGAAAATTAATATGTTTGGATTTTCAGTTCCAGCTTTACTTGCTGTCTCCAAG
Protein sequence

>Msa0422100

MRGRMSHGVMALGRLTEQLLKQGKSCGDSIQNCFTSLMEVSIQLKELQDGKGDEVEDEDGVDEDSDDDDSEDYDEKINDVEKINMFGFSVPALLAVSK