AlfalfaGEDB | Msa0422100

M. sativa cultivar zhongmu-4 / Msa0422100

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0422100	PNY05988.1	86.364	44	6	0	9	52	643	686	1.95e-15	81.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0422100	A0A2K3NSH5	86.364	44	6	0	9	52	643	686	9.31e-16	81.6

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0422100	0.812368	4.390539e-51	-8.615850e-47
Msa0078900	Msa0422100	0.810402	1.173716e-50	-8.615850e-47
Msa0157290	Msa0422100	0.812657	3.797723e-51	-8.615850e-47
Msa0165220	Msa0422100	0.800497	1.401918e-48	-8.615850e-47
Msa0288100	Msa0422100	0.813591	2.369084e-51	-8.615850e-47
Msa0321310	Msa0422100	0.801670	8.068070e-49	-8.615850e-47
Msa0330340	Msa0422100	0.803119	4.058812e-49	-8.615850e-47
Msa0395790	Msa0422100	0.818086	2.354693e-52	-8.615850e-47
Msa0422100	Msa0424320	0.833130	6.364294e-56	-8.615850e-47
Msa0422100	Msa0443260	0.816629	5.011170e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0422100	MtrunA17_Chr4g0069091	88.636	44	5	0	9	52	932	975	4.12e-19	80.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 48 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCCATGGACAAATGAG+AGG	0.628002	3_2:-71233705	Msa0422100:exon
AAACTGCTTCACTTCACTAA+TGG	0.340507	3_2:-71233516	Msa0422100:CDS
AAAGAATTACAAGATGGGAA+AGG	0.585242	3_2:-71233476	Msa0422100:CDS
AAAGCCATGGACAAATGAGA+GGG	0.669427	3_2:-71233704	Msa0422100:exon
AAATAAAATTAGATTGGATC+TGG	0.410449	3_2:-71233783	Msa0422100:five_prime_UTR
AAATCTAAATAAAATTAGAT+TGG	0.429845	3_2:-71233789	Msa0422100:five_prime_UTR
ACAAATCCTCATCTATCTGA+TGG	0.493937	3_2:-71234022	Msa0422100:five_prime_UTR
ACATGAGGAGACAACGTTGG+TGG	0.586011	3_2:+71234049	None:intergenic
ACTGAGCAACTCCTGAAACA+AGG	0.580659	3_2:-71233563	Msa0422100:CDS
AGAACATGAGGAGACAACGT+TGG	0.772358	3_2:+71234046	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAACCCTCTATTTACAATT+TGG	+	chr3_2:71233010-71233029	None:intergenic	30.0%
TTCTCCACTAAAATCTCAAA+AGG	+	chr3_2:71233101-71233120	None:intergenic	30.0%
CTGGAAAATTCTACAGAAAA+CGG	-	chr3_2:71233215-71233234	Msa0422100:intron	30.0%
TGGAAAATTCTACAGAAAAC+GGG	-	chr3_2:71233216-71233235	Msa0422100:intron	30.0%
AAACCAAAAACGGAAAATTC+TGG	-	chr3_2:71233250-71233269	Msa0422100:intron	30.0%
CAGTTGAAAGAATTACAAGA+TGG	-	chr3_2:71233497-71233516	Msa0422100:CDS	30.0%
AAAGAATTACAAGATGGGAA+AGG	-	chr3_2:71233503-71233522	Msa0422100:CDS	30.0%
TTTAACAGTTCTTAATCCTG+TGG	-	chr3_2:71233715-71233734	Msa0422100:five_prime_UTR	30.0%
TTAACAGTTCTTAATCCTGT+GGG	-	chr3_2:71233716-71233735	Msa0422100:five_prime_UTR	30.0%
TAAACGGAAGAAGAATTTCT+TGG	-	chr3_2:71233777-71233796	Msa0422100:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	71232914	71234087	71232914	ID=Msa0422100;Name=Msa0422100
chr3_2	mRNA	71232914	71234087	71232914	ID=Msa0422100-mRNA-1;Parent=Msa0422100;Name=Msa0422100-mRNA-1;_AED=0.28;_eAED=0.35;_QI=375\|0.5\|0.66\|1\|1\|1\|3\|0\|98
chr3_2	exon	71232914	71232982	71232914	ID=Msa0422100-mRNA-1:exon:17375;Parent=Msa0422100-mRNA-1
chr3_2	exon	71233406	71233605	71233406	ID=Msa0422100-mRNA-1:exon:17374;Parent=Msa0422100-mRNA-1
chr3_2	exon	71233688	71234087	71233688	ID=Msa0422100-mRNA-1:exon:17373;Parent=Msa0422100-mRNA-1
chr3_2	five_prime_UTR	71233713	71234087	71233713	ID=Msa0422100-mRNA-1:five_prime_utr;Parent=Msa0422100-mRNA-1
chr3_2	CDS	71233688	71233712	71233688	ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1
chr3_2	CDS	71233406	71233605	71233406	ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1
chr3_2	CDS	71232914	71232982	71232914	ID=Msa0422100-mRNA-1:cds;Parent=Msa0422100-mRNA-1

Gene Sequence	>Msa0422100 ATGAGAGGGAGAATGAGCCATGGAGTGATGGCACTAGGTCGATTGACTGAGCAACTCCTGAAACAAGGGAAGTCGTGTGGTGATTCAATTCAAAACTGCTTCACTTCACTAATGGAGGTATCCATTCAGTTGAAAGAATTACAAGATGGGAAAGGAGATGAAGTAGAGGATGAAGATGGCGTGGATGAGGATAGTGACGATGATGACTCCGAAGATTATGATGAGAAAATTAATGATGTTGAGAAAATTAATATGTTTGGATTTTCAGTTCCAGCTTTACTTGCTGTCTCCAAG
Protein sequence	>Msa0422100 MRGRMSHGVMALGRLTEQLLKQGKSCGDSIQNCFTSLMEVSIQLKELQDGKGDEVEDEDGVDEDSDDDDSEDYDEKINDVEKINMFGFSVPALLAVSK