AlfalfaGEDB | Msa0643380

M. sativa cultivar zhongmu-4 / Msa0643380

KEGG: -
GO: -
PFAM: PF00561.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643380	XP_003606488.1	97.527	364	9	0	1	364	126	489	0.0	749

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0643380	0.801110	1.050753e-48	-8.615850e-47
Msa0024500	Msa0643380	0.811060	8.455702e-51	-8.615850e-47
Msa0050320	Msa0643380	0.813434	2.564543e-51	-8.615850e-47
Msa0081700	Msa0643380	0.803374	3.593269e-49	-8.615850e-47
Msa0120320	Msa0643380	0.804774	1.838071e-49	-8.615850e-47
Msa0154720	Msa0643380	0.810885	9.228848e-51	-8.615850e-47
Msa0174060	Msa0643380	0.811359	7.284656e-51	-8.615850e-47
Msa0225070	Msa0643380	0.813210	2.872515e-51	-8.615850e-47
Msa0378310	Msa0643380	0.805704	1.174142e-49	-8.615850e-47
Msa0457070	Msa0643380	0.805809	1.115709e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643380	MtrunA17_Chr4g0029971	97.527	364	9	0	1	364	126	489	0.0	749
Msa0643380	MtrunA17_Chr7g0263721	60.000	350	133	3	1	348	158	502	7.20e-162	463
Msa0643380	MtrunA17_Chr4g0026421	32.915	319	166	9	2	309	50	331	7.92e-42	149
Msa0643380	MtrunA17_Chr3g0136021	32.177	317	171	11	2	309	133	414	2.63e-40	147
Msa0643380	MtrunA17_Chr1g0178741	31.290	310	179	7	2	305	101	382	6.98e-39	142
Msa0643380	MtrunA17_Chr2g0321341	30.408	319	190	9	2	318	44	332	3.96e-38	139
Msa0643380	MtrunA17_Chr2g0321351	31.307	329	186	11	2	324	52	346	4.61e-38	140
Msa0643380	MtrunA17_Chr1g0178721	30.000	330	195	8	2	325	76	375	1.88e-37	138
Msa0643380	MtrunA17_Chr1g0171181	44.094	127	68	2	2	125	44	170	4.12e-27	109

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0643380	AT3G09690.1	64.865	333	115	2	2	332	168	500	9.54e-166	474
Msa0643380	AT3G09690.2	64.865	333	115	2	2	332	168	500	9.54e-166	474
Msa0643380	AT5G02970.1	59.654	347	138	2	2	346	165	511	1.67e-158	455
Msa0643380	AT3G44510.2	31.707	328	183	10	2	319	11	307	1.75e-39	143
Msa0643380	AT5G22460.2	30.225	311	178	7	2	305	44	322	2.00e-38	140
Msa0643380	AT5G22460.3	30.225	311	178	7	2	305	44	322	2.00e-38	140
Msa0643380	AT5G22460.1	30.225	311	178	7	2	305	44	322	2.00e-38	140
Msa0643380	AT3G54240.1	27.356	329	208	6	2	327	48	348	2.74e-37	137
Msa0643380	AT1G74280.1	29.283	321	171	6	2	305	64	345	6.29e-37	137
Msa0643380	AT2G36290.2	31.494	308	181	8	2	305	46	327	9.14e-36	134

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Find 110 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATATGTATGTGAAA+TGG	0.431214	4_3:+46289141	None:intergenic
AAAATACTTGCTTCTCAAGT+GGG	0.518790	4_3:+46291764	None:intergenic
AAAGAAATGCTGAGGCTGAG+AGG	0.645314	4_3:+46289065	None:intergenic
AAAGCAGCTTCCTCCACAAA+GGG	0.525187	4_3:+46290179	None:intergenic
AAATACTTGCTTCTCAAGTG+GGG	0.651959	4_3:+46291765	None:intergenic
AAATGTGAAACCCTTTGTGG+AGG	0.646017	4_3:-46290189	Msa0643380:CDS
AACCTGCAAGCCGAGAGGAA+AGG	0.606711	4_3:+46291626	None:intergenic
AAGAAATGCTGAGGCTGAGA+GGG	0.556389	4_3:+46289066	None:intergenic
AAGTGGAAGCAAGCATGCTT+GGG	0.466214	4_3:-46291318	Msa0643380:CDS
AAGTTCAACGTTGGCTTCTA+GGG	0.458277	4_3:+46289025	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATACTTGCTTCTCAAGT+GGG	+	chr4_3:46288800-46288819	None:intergenic	30.0%
AAAAATACTTGCTTCTCAAG+TGG	+	chr4_3:46288801-46288820	None:intergenic	30.0%
GATGTTGTGTATTAACTTTC+AGG	-	chr4_3:46289783-46289802	Msa0643380:intron	30.0%
TTGTCATCAAAGGATCATAT+GGG	+	chr4_3:46289836-46289855	None:intergenic	30.0%
TTTGTCATCAAAGGATCATA+TGG	+	chr4_3:46289837-46289856	None:intergenic	30.0%
TTAGTCAACATGTCCATATA+TGG	-	chr4_3:46290053-46290072	Msa0643380:CDS	30.0%
ATGTACATAGATAGAAATGC+AGG	+	chr4_3:46290089-46290108	None:intergenic	30.0%
TGGATGCTGATAAGAATTTA+TGG	-	chr4_3:46290138-46290157	Msa0643380:CDS	30.0%
TGCAGAAACGAAAATACAAA+AGG	-	chr4_3:46290446-46290465	Msa0643380:intron	30.0%
ACAAAAGGAATGTGCTTAAT+TGG	-	chr4_3:46290461-46290480	Msa0643380:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	46288688	46291895	46288688	ID=Msa0643380;Name=Msa0643380
chr4_3	mRNA	46288688	46291895	46288688	ID=Msa0643380-mRNA-1;Parent=Msa0643380;Name=Msa0643380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=158\|1\|1\|1\|1\|1\|5\|289\|364
chr4_3	exon	46288688	46289255	46288688	ID=Msa0643380-mRNA-1:exon:10776;Parent=Msa0643380-mRNA-1
chr4_3	exon	46290034	46290285	46290034	ID=Msa0643380-mRNA-1:exon:10775;Parent=Msa0643380-mRNA-1
chr4_3	exon	46290561	46290778	46290561	ID=Msa0643380-mRNA-1:exon:10774;Parent=Msa0643380-mRNA-1
chr4_3	exon	46291285	46291521	46291285	ID=Msa0643380-mRNA-1:exon:10773;Parent=Msa0643380-mRNA-1
chr4_3	exon	46291629	46291895	46291629	ID=Msa0643380-mRNA-1:exon:10772;Parent=Msa0643380-mRNA-1
chr4_3	five_prime_UTR	46291738	46291895	46291738	ID=Msa0643380-mRNA-1:five_prime_utr;Parent=Msa0643380-mRNA-1
chr4_3	CDS	46291629	46291737	46291629	ID=Msa0643380-mRNA-1:cds;Parent=Msa0643380-mRNA-1
chr4_3	CDS	46291285	46291521	46291285	ID=Msa0643380-mRNA-1:cds;Parent=Msa0643380-mRNA-1
chr4_3	CDS	46290561	46290778	46290561	ID=Msa0643380-mRNA-1:cds;Parent=Msa0643380-mRNA-1
chr4_3	CDS	46290034	46290285	46290034	ID=Msa0643380-mRNA-1:cds;Parent=Msa0643380-mRNA-1
chr4_3	CDS	46288977	46289255	46288977	ID=Msa0643380-mRNA-1:cds;Parent=Msa0643380-mRNA-1
chr4_3	three_prime_UTR	46288688	46288976	46288688	ID=Msa0643380-mRNA-1:three_prime_utr;Parent=Msa0643380-mRNA-1

Gene Sequence

>Msa0643380

ATGCTTCCGGATGGAAGATACATGGCATACAAGGAGCAAGGTGTTTCGGCTGACAAAGCAAGATTTTCGATTATTGCTCCTCATACTTTCCTTTCCTCTCGGCTTGCAGGAATTCCTGGTGTTAAAGATTCACTGATGGAAGAATTTGGCGTTCACTTGATTACTTATGATCTGCCTGGTTTTGGTGAGAGCGATCCTCATCCCAAACGAAATCTGGAATCGTCAGCAATAGATATGTCGTTTTTAGCTGATGCTCTTGGTGTTGACAAGTTTTGGATCATTGGTTACTCAAGTGGAAGCAAGCATGCTTGGGCTGCGCTTAGATACATACCTGATAGACTTGCAGGTGCTGCCATGTTTGCCCCCATGGTGAACCCATATGATCCTTTGATGACAAACGAAGAGAGACGAAAAACCTGGAACAAATGGACGCGAAAAAGAAAATTGATGTACTTCTTAGCTCGAAGCTTTCCTAGACTTCTTGGTTTCTTCTATCAGCGAAGTTTCTTATCAGGGAAGCATGGTCAGATTGACAGGTGGCTGTCTTTATCTCTTGGAAAGAGGGACAAAGCACTGATGGAAGATCCAATCTATGAGGATTTCTGGCAAAGGGATGTGGAAGAATCAATTAGGCAAGGAAATGTGAAACCCTTTGTGGAGGAAGCTGCTTTGCAGGTCTCGAATTGGGGTTTCAGCCTTTTAGACCTCAAGTTGCAGAAACGAAAATACAAAAGGAATGTGCTTAATTGGCTTAAGGCAATTTTCACTGAAACTCAGGAGTATACGGGATTTCTCGGTCCAATACACATATGGCAAGGAATGGATGACAAAGTGGTTCCCCCGTCAATGACTGATTTTGTGCATCGCGTACTACCTGGAGCTGCAGTACACAAACTCCCATATGAAGGCCATTTCACATACATATACTTTTGTGATGAATGCCACAGACAGATATTTACTACACTTTTTGGAACCCCGCAAGGCCCTCTCAGCCTCAGCATTTCTTTAGAAGTAGATCCAATTACCCTAGAAGCCAACGTTGAACTTGAACAGCAAGAAGTAGTAGCAAGTAGTGGTGATTATGCCACTGATTAG

Protein sequence

>Msa0643380

MLPDGRYMAYKEQGVSADKARFSIIAPHTFLSSRLAGIPGVKDSLMEEFGVHLITYDLPGFGESDPHPKRNLESSAIDMSFLADALGVDKFWIIGYSSGSKHAWAALRYIPDRLAGAAMFAPMVNPYDPLMTNEERRKTWNKWTRKRKLMYFLARSFPRLLGFFYQRSFLSGKHGQIDRWLSLSLGKRDKALMEDPIYEDFWQRDVEESIRQGNVKPFVEEAALQVSNWGFSLLDLKLQKRKYKRNVLNWLKAIFTETQEYTGFLGPIHIWQGMDDKVVPPSMTDFVHRVLPGAAVHKLPYEGHFTYIYFCDECHRQIFTTLFGTPQGPLSLSISLEVDPITLEANVELEQQEVVASSGDYATD*